MD09G1216000.v1.1

leucine-rich repeat extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007583
Physical Location & Seq
Forward (+)
21379562 .. 21380743
1182 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1216000.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGAGTTCGGCGTTGAAGCAGGCCATTCTCTCCGACCCGTTCAACATCACGGGCAATTGGGTCGGATCTAATGTCTGCAAGTACACCAGAGTTTTTGGCACCAAGGCGCTTGACAACAGAACGATCCGGACTGTGGCGGGGATCGACCTCAACCACGGTGACATTGCCGGGTACTTACCGGAGGAGCTTGGGCTACTCACGGATCTCGCATTGTTCCACATCAACTCCAACAGGTTCTGTGGAACTGTTCCTCACAAGTTCAACCGCCTCAAGCTGTTGTTCGAGCTCGATTTGAGCAACAATCGGTTCGCCGGAAAGTTCCCTCGGGTGGTTCTCCAGCTGCCAAAGCTGAAATTCTTGGATCTGAGGTTCAACGAGTTCGAAGGGATGGTGCCGAAGGAGCTGTTCGACAAGGACTTGGACGCCATTTTCATCAACCATAACCGGTTCCGGTTCGATCTGCCCGATAACTTCAGGAACTCACCGGTTTCCGTCATCGTCCTCGCCAACAACAAGTTCCACGGCTGCGATCCGGCTAGTTTGGGGAACATGTCAAACCTCAACGAGATTATCCTGATGAACAATGGATTCCAGTCGTGTTTGCCGCCGGAGATTAGGATGCTGAAGAACTTGACGGTCTCAATGTGGCCCACAAACTTGCTGTCGGGGAAGATTTCGGCGAGTATTTGTTCACTGCCGAGTCTGCAGAATTTCACTTACTCGTATAACTTCTTCACGGGTGAGCCGCCGGCGTGTTTAGGACTGGCGGGCTTTGACGACAAGAGGAATTGCTTGCCGAATAGGCCAGCGCAGCGAACGGCAGCGCAGTGTAAGTCGTTCTTGTCGAAGCCTGTGGATTGTATTTCTTTTAGATGTAAGCCTTTTGTTCCGTCTTTGCCTACTCCTCACTCCACTCAAACCCGACCTGCTGCACATGGTGCGCACACCCGGAGCAAGTTGGGCTGCGCTCTCGGCATCGACTCCTTCCACCGCAGCGCCTGGCCTCCCTCGAACTCATCACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005199 GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8

Protein Analysis

342

Amino Acids

37.97

Weight (kDa)

9.22

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 69 - 126 5e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 942
Acc36I ACCTGC 1 cut(s) 934
AccB1I GGYRCC 2 cut(s) 98, 391
AccIII TCCGGA 1 cut(s) 126
AciI CCGC 6 cut(s) 137, 265, 605, 746, 767, 991
AclWI GGATC 6 cut(s) 73, 118, 149, 210, 369, 524
AcsI RAATTY 2 cut(s) 353, 709
AcuI CTGAAG 2 cut(s) 457, 644
AcyI GRCGYC 1 cut(s) 423
AdeI CACNNNGTG 1 cut(s) 938
AfaI GTAC 2 cut(s) 83, 173
AfiI CCNNNNNNNGG 2 cut(s) 133, 328
AflIII ACRYGT 1 cut(s) 549
AgeI ACCGGT 2 cut(s) 444, 484
AgsI TTSAA 4 cut(s) 16, 43, 262, 373
AjnI CCWGG 1 cut(s) 998
AjuI GAANNNNNNNTTGG 2 cut(s) 500, 532
AloI GAACNNNNNNTCC 2 cut(s) 353, 385
AluBI AGCT 6 cut(s) 187, 274, 286, 340, 349, 403
AluI AGCT 6 cut(s) 187, 274, 286, 340, 349, 403
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 1 cut(s) 643
AlwI GGATC 6 cut(s) 73, 118, 149, 210, 369, 524
AlwNI CAGNNNCTG 2 cut(s) 237, 999
Ama87I CYCGRG 1 cut(s) 324
Aor13HI TCCGGA 1 cut(s) 126
AoxI GGCC 4 cut(s) 21, 647, 803, 1001
ApeKI GCWGC 7 cut(s) 340, 525, 811, 821, 929, 963, 993
ApoI RAATTY 2 cut(s) 353, 709
ArsI GACNNNNNNTTYG 2 cut(s) 647, 679
AsiGI ACCGGT 2 cut(s) 444, 484
AspLEI GCGC 6 cut(s) 109, 811, 826, 943, 968, 998
AspS9I GGNCC 1 cut(s) 648
AsuC2I CCSGG 2 cut(s) 169, 949
AsuHPI GGTGA 3 cut(s) 170, 474, 752
AsuII TTCGAA 1 cut(s) 381
AvaI CYCGRG 1 cut(s) 324
BanI GGYRCC 2 cut(s) 98, 391
BanII GRGCYC 1 cut(s) 288
BarI GAAGNNNNNNTAC 2 cut(s) 716, 748
Bbv12I GWGCWC 1 cut(s) 288
BbvI GCAGC 7 cut(s) 327, 512, 823, 833, 916, 950, 1005
BccI CCATC 1 cut(s) 382
BceAI ACGGC 2 cut(s) 538, 834
BcgI CGANNNNNNTGC 2 cut(s) 43, 77
BciT130I CCWGG 1 cut(s) 1000
BcnI CCSGG 2 cut(s) 169, 949
BcoDI GTCTC 1 cut(s) 643
BfaI CTAG 1 cut(s) 537
BfmI CTRYAG 1 cut(s) 704
BfoI RGCGCY 2 cut(s) 110, 999
BfuAI ACCTGC 1 cut(s) 934
BglI GCCNNNNNGGC 1 cut(s) 802
BisI GCNGC 9 cut(s) 341, 526, 605, 746, 812, 822, 930, 964, 994
BlsI GCNGC 9 cut(s) 342, 527, 606, 747, 813, 823, 931, 965, 995
Bme1390I CCNGG 3 cut(s) 169, 949, 1000
BmeT110I CYCGRG 1 cut(s) 324
BmgT120I GGNCC 1 cut(s) 648
BmiI GGNNCC 3 cut(s) 100, 393, 449
BmrFI CCNGG 3 cut(s) 169, 949, 1000
BmsI GCATC 2 cut(s) 609, 984
BpmI CTGGAG 1 cut(s) 320
Bpu14I TTCGAA 1 cut(s) 381
BpuEI CTTGAG 1 cut(s) 254
BpuMI CCSGG 2 cut(s) 169, 949
BsaHI GRCGYC 1 cut(s) 423
BsaI GGTCTC 1 cut(s) 643
BsaJI CCNNGG 4 cut(s) 102, 154, 323, 520
BsaWI WCCGGW 5 cut(s) 126, 178, 444, 450, 484
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 328
Bse118I RCCGGY 3 cut(s) 444, 484, 748
Bse1I ACTGG 2 cut(s) 592, 768
Bse3DI GCAATG 1 cut(s) 162
BseAI TCCGGA 1 cut(s) 126
BseBI CCWGG 1 cut(s) 1000
BseDI CCNNGG 4 cut(s) 102, 154, 323, 520
BseGI GGATG 2 cut(s) 393, 624
BseLI CCNNNNNNNGG 2 cut(s) 133, 328
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 1 cut(s) 356
BseNI ACTGG 2 cut(s) 592, 768
BseRI GAGGAG 2 cut(s) 197, 894
BseXI GCAGC 7 cut(s) 327, 512, 823, 833, 916, 950, 1005
BsgI GTGCAG 1 cut(s) 915
BshFI GGCC 4 cut(s) 23, 649, 805, 1003
BshNI GGYRCC 2 cut(s) 98, 391
BshTI ACCGGT 2 cut(s) 444, 484
BsiHKAI GWGCWC 1 cut(s) 288
BsiHKCI CYCGRG 1 cut(s) 324
BslI CCNNNNNNNGG 2 cut(s) 133, 328
BsmAI GTCTC 1 cut(s) 643
BsnI GGCC 4 cut(s) 23, 649, 805, 1003
Bso31I GGTCTC 1 cut(s) 643
BsoBI CYCGRG 1 cut(s) 324
Bsp119I TTCGAA 1 cut(s) 381
Bsp1286I GDGCHC 1 cut(s) 288
Bsp13I TCCGGA 1 cut(s) 126
Bsp143I GATC 7 cut(s) 65, 123, 141, 202, 361, 457, 529
BspACI CCGC 6 cut(s) 137, 265, 605, 746, 767, 991
BspANI GGCC 4 cut(s) 23, 649, 805, 1003
BspCNI CTCAG 1 cut(s) 357
BspEI TCCGGA 1 cut(s) 126
BspLI GGNNCC 3 cut(s) 100, 393, 449
BspMAI CTGCAG 1 cut(s) 708
BspMI ACCTGC 1 cut(s) 934
BspPI GGATC 6 cut(s) 73, 118, 149, 210, 369, 524
BspT104I TTCGAA 1 cut(s) 381
BspT107I GGYRCC 2 cut(s) 98, 391
BspTNI GGTCTC 1 cut(s) 643
BsrDI GCAATG 1 cut(s) 162
BsrFI RCCGGY 3 cut(s) 444, 484, 748
BsrI ACTGG 2 cut(s) 592, 768
BssAI RCCGGY 3 cut(s) 444, 484, 748
BssECI CCNNGG 4 cut(s) 102, 154, 323, 520
BssMI GATC 7 cut(s) 65, 123, 141, 202, 361, 457, 529
BssNI GRCGYC 1 cut(s) 423
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 1 cut(s) 1000
Bst4CI ACNGT 4 cut(s) 133, 158, 247, 637
BstACI GRCGYC 1 cut(s) 423
BstAPI GCANNNNNTGC 1 cut(s) 938
BstBI TTCGAA 1 cut(s) 381
BstC8I GCNNGC 5 cut(s) 21, 750, 769, 794, 807
BstDEI CTNAG 1 cut(s) 365
BstDSI CCRYGG 2 cut(s) 154, 520
BstF5I GGATG 2 cut(s) 393, 624
BstH2I RGCGCY 2 cut(s) 110, 999
BstHHI GCGC 6 cut(s) 109, 811, 826, 943, 968, 998
BstKTI GATC 7 cut(s) 68, 126, 144, 205, 364, 460, 532
BstMAI GTCTC 1 cut(s) 643
BstMBI GATC 7 cut(s) 65, 123, 141, 202, 361, 457, 529
BstMWI GCNNNNNNNGC 8 cut(s) 346, 400, 703, 802, 811, 938, 960, 972
BstNI CCWGG 1 cut(s) 1000
BstNSI RCATGY 1 cut(s) 553
BstSCI CCNGG 3 cut(s) 167, 947, 998
BstSFI CTRYAG 1 cut(s) 704
BstV1I GCAGC 7 cut(s) 327, 512, 823, 833, 916, 950, 1005
BstX2I RGATCY 3 cut(s) 65, 202, 361
BstYI RGATCY 3 cut(s) 65, 202, 361
BsuRI GGCC 4 cut(s) 23, 649, 805, 1003
BtgI CCRYGG 2 cut(s) 154, 520
BtsCI GGATG 2 cut(s) 393, 624
BtsI GCAGTG 2 cut(s) 692, 833
BtsIMutI CAGTG 2 cut(s) 692, 833
BveI ACCTGC 1 cut(s) 934
Cac8I GCNNGC 5 cut(s) 21, 750, 769, 794, 807
CaiI CAGNNNCTG 2 cut(s) 237, 999
CfoI GCGC 6 cut(s) 109, 811, 826, 943, 968, 998
Cfr10I RCCGGY 3 cut(s) 444, 484, 748
Cfr13I GGNCC 1 cut(s) 648
CseI GACGC 1 cut(s) 431
Csp6I GTAC 2 cut(s) 82, 172
CspAI ACCGGT 2 cut(s) 444, 484
CviAII CATG 2 cut(s) 550, 935
CviQI GTAC 2 cut(s) 82, 172
DdeI CTNAG 1 cut(s) 365
DpnI GATC 7 cut(s) 67, 125, 143, 204, 363, 459, 531
DpnII GATC 7 cut(s) 65, 123, 141, 202, 361, 457, 529
DraIII CACNNNGTG 1 cut(s) 938
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 102
Eco24I GRGCYC 1 cut(s) 288
Eco31I GGTCTC 1 cut(s) 643
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 2 cut(s) 457, 644
Eco88I CYCGRG 1 cut(s) 324
EcoICRI GAGCTC 1 cut(s) 286
EcoRII CCWGG 1 cut(s) 998
EcoT14I CCWWGG 1 cut(s) 102
EcoT38I GRGCYC 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 2 cut(s) 553, 938
FaiI YATR 5 cut(s) 441, 551, 726, 936, 1024
FalI AAGNNNNNCTT 2 cut(s) 824, 856
FatI CATG 2 cut(s) 549, 934
FauI CCCGC 2 cut(s) 130, 760
Fnu4HI GCNGC 9 cut(s) 341, 526, 605, 746, 812, 822, 930, 964, 994
FokI GGATG 2 cut(s) 400, 631
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 9 cut(s) 341, 526, 605, 746, 812, 822, 930, 964, 994
FspAI RTGCGCAY 1 cut(s) 942
FspBI CTAG 1 cut(s) 537
FspI TGCGCA 1 cut(s) 942
GlaI GCGC 6 cut(s) 108, 810, 825, 942, 967, 997
GluI GCNGC 9 cut(s) 341, 526, 605, 746, 812, 822, 930, 964, 994
GsuI CTGGAG 1 cut(s) 320
HaeII RGCGCY 2 cut(s) 110, 999
HaeIII GGCC 4 cut(s) 23, 649, 805, 1003
HgaI GACGC 1 cut(s) 431
HhaI GCGC 6 cut(s) 109, 811, 826, 943, 968, 998
Hin1I GRCGYC 1 cut(s) 423
Hin1II CATG 2 cut(s) 553, 938
Hin6I GCGC 6 cut(s) 107, 809, 824, 941, 966, 996
HinP1I GCGC 6 cut(s) 107, 809, 824, 941, 966, 996
HinfI GANTC 3 cut(s) 588, 700, 980
HphI GGTGA 3 cut(s) 170, 474, 752
Hpy166II GTNNAC 2 cut(s) 84, 692
Hpy188I TCNGA 3 cut(s) 34, 65, 366
Hpy188III TCNNGA 3 cut(s) 127, 475, 574
Hpy8I GTNNAC 2 cut(s) 84, 692
HpyAV CCTTC 3 cut(s) 377, 391, 994
HpyCH4III ACNGT 4 cut(s) 133, 158, 247, 637
HpyCH4V TGCA 3 cut(s) 78, 706, 932
HpyF10VI GCNNNNNNNGC 8 cut(s) 346, 400, 703, 802, 811, 938, 960, 972
HpyF3I CTNAG 1 cut(s) 365
Hsp92I GRCGYC 1 cut(s) 423
Hsp92II CATG 2 cut(s) 553, 938
HspAI GCGC 6 cut(s) 107, 809, 824, 941, 966, 996
Kpn2I TCCGGA 1 cut(s) 126
KroI GCCGGC 1 cut(s) 748
KroNI GCCGGC 1 cut(s) 750
Kzo9I GATC 7 cut(s) 65, 123, 141, 202, 361, 457, 529
LmnI GCTCC 3 cut(s) 184, 400, 951
Lsp1109I GCAGC 7 cut(s) 327, 512, 823, 833, 916, 950, 1005
LweI GCATC 2 cut(s) 609, 984
MaeI CTAG 1 cut(s) 537
MaeIII GTNAC 1 cut(s) 158
MalI GATC 7 cut(s) 67, 125, 143, 204, 363, 459, 531
MboI GATC 7 cut(s) 65, 123, 141, 202, 361, 457, 529
MboII GAAGA 3 cut(s) 637, 682, 724
MfeI CAATTG 1 cut(s) 55
MflI RGATCY 3 cut(s) 65, 202, 361
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 4 cut(s) 55, 353, 709, 787
MlyI GAGTC 2 cut(s) 709, 974
MmeI TCCRAC 3 cut(s) 43, 57, 252
MreI CGCCGGCG 1 cut(s) 748
MroI TCCGGA 1 cut(s) 126
MroNI GCCGGC 1 cut(s) 748
MslI CAYNNNNRTG 1 cut(s) 1021
MspA1I CMGCKG 1 cut(s) 340
MspR9I CCNGG 3 cut(s) 169, 949, 1000
MunI CAATTG 1 cut(s) 55
MvaI CCWGG 1 cut(s) 1000
MwoI GCNNNNNNNGC 8 cut(s) 346, 400, 703, 802, 811, 938, 960, 972
NaeI GCCGGC 1 cut(s) 750
NciI CCSGG 2 cut(s) 169, 949
NdeII GATC 7 cut(s) 65, 123, 141, 202, 361, 457, 529
NgoMIV GCCGGC 1 cut(s) 748
NlaIII CATG 2 cut(s) 553, 938
NlaIV GGNNCC 3 cut(s) 100, 393, 449
NmeAIII GCCGAG 2 cut(s) 723, 951
NmuCI GTSAC 1 cut(s) 158
NsbI TGCGCA 1 cut(s) 942
NspI RCATGY 1 cut(s) 553
NspV TTCGAA 1 cut(s) 381
PciI ACATGT 1 cut(s) 549
PcsI WCGNNNNNNNCGW 2 cut(s) 462, 842
PdiI GCCGGC 1 cut(s) 750
PfeI GAWTC 1 cut(s) 588
PinAI ACCGGT 2 cut(s) 444, 484
PkrI GCNGC 9 cut(s) 342, 527, 606, 747, 813, 823, 931, 965, 995
PleI GAGTC 2 cut(s) 708, 974
PpsI GAGTC 2 cut(s) 708, 974
PscI ACATGT 1 cut(s) 549
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 998
PspGI CCWGG 1 cut(s) 998
PspN4I GGNNCC 3 cut(s) 100, 393, 449
PspPI GGNCC 1 cut(s) 648
PstI CTGCAG 1 cut(s) 708
PstNI CAGNNNCTG 2 cut(s) 237, 999
PsuI RGATCY 3 cut(s) 65, 202, 361
PvuII CAGCTG 1 cut(s) 340
RsaI GTAC 2 cut(s) 83, 173
RsaNI GTAC 2 cut(s) 82, 172
RseI CAYNNNNRTG 1 cut(s) 1021
SacI GAGCTC 1 cut(s) 288
SatI GCNGC 9 cut(s) 341, 526, 605, 746, 812, 822, 930, 964, 994
Sau3AI GATC 7 cut(s) 65, 123, 141, 202, 361, 457, 529
Sau96I GGNCC 1 cut(s) 648
SchI GAGTC 2 cut(s) 709, 974
ScrFI CCNGG 3 cut(s) 169, 949, 1000
SduI GDGCHC 1 cut(s) 288
SfaNI GCATC 2 cut(s) 609, 984
SfcI CTRYAG 1 cut(s) 704
SfuI TTCGAA 1 cut(s) 381
SgrAI CRCCGGYG 1 cut(s) 748
SmiMI CAYNNNNRTG 1 cut(s) 1021
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 4 cut(s) 55, 353, 709, 787
SsiI CCGC 6 cut(s) 137, 265, 605, 746, 767, 991
SspMI CTAG 1 cut(s) 537
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 3 cut(s) 167, 947, 998
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 4 cut(s) 133, 158, 247, 637
TaqI TCGA 9 cut(s) 144, 282, 288, 381, 408, 456, 845, 978, 1010
TasI AATT 4 cut(s) 55, 353, 709, 787
TatI WGTACW 1 cut(s) 81
TauI GCSGC 2 cut(s) 607, 748
TfiI GAWTC 1 cut(s) 588
TscAI CASTG 2 cut(s) 699, 833
TseFI GTSAC 1 cut(s) 158
TseI GCWGC 7 cut(s) 340, 525, 811, 821, 929, 963, 993
Tsp45I GTSAC 1 cut(s) 158
TspDTI ATGAA 2 cut(s) 421, 593
TspGWI ACGGA 3 cut(s) 215, 481, 879
TspRI CASTG 2 cut(s) 699, 833
XapI RAATTY 2 cut(s) 353, 709
XceI RCATGY 1 cut(s) 553
XspI CTAG 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.