Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0007583

Physical Location & Seq

Genomic Coordinates Forward (+)

14870452 .. 14871759

Gene Structure 1308 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1188400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1308 bp

ATGAAGAAGAAGACCACCACCCTCTTCCACCTCTCACTCCTCACTGCCCTTTTCCTTGCCGCGACATCACTCTTCTCTGGTGCTGCTGCTCAGGAGCACCACCACCAGCACCGCCGCAGTAGCCACATTGTCAGCCATGGACCTCTCTCCGACGAGGAAGCCCTCTACATCAAGCACCGCCAGCTCCTCTACTACCGCGATGAGTTCGGTGACCGCGGCGAGCATGTGACGGTCGACCCCTCGCTGGTCTTCGAGAACGACCGCCTCCGGAACGCTTACATAGCTCTGCAGGCGTTGAAGCAGGCCATTCTCTCCGACCCGTTCAACATCACGGGCAATTGGGTCGGATCTAATGTCTGCAAGTACACCGGAGTTTTCTGCACCAAGGCGCTTGACAACAGAACGATCCGGACTGTGGCGGGGATCGACCTCAACCACGGCGACATAGCCGGGTACTTGCCGGAGGAGCTTGGGCTGCTCACGGATCTCGCATTGTTCCACATCAACTCCAACAGGTTCTGTGGAACTGTTCCTCACAAGTTCAACCGCCTCAAGCTGCTGTTCGAGCTCGATTTGAGCAACAATCGGTTCGCCGGAAAGTTCCCTCGGGTGGTTCTCCAGCTGCCGAAGCTGAAATTCTTGGATCTGAGGTTCAACGAGTTCGAAGGGACGGTGCCAAAGGAGCTGTTCGACAAGGACTTGGACGCCATTTTCATCAACCACAACCGGTTCCGGTTCGATCTGCCTGATAACTTCGGGAATTCGCCGGTCTCCGTCATCGTCCTCGCCAACAACAAGTTCCACGGCTGCGTTCCGGCTAGTTTGGGGAACATGTCAAACCTCAACGAGATTATCCTGATGAACAATGGATTCCGGTCGTGTTTGCCGCCGGAGATTGGGATGCTGAAGAACTTGACTGTGTTTGACGTCAGCTTCAACCAGTTTCTGGGGTCGCTACCGGAGACGATTGGAGGGATGGTGAGCTTGGAGCAGCTCAATGTGGCCCACAACTTGCTGTCGGGGAAGATTCCGGCGAGTATTTGTTCGCTGCCGAGTCTGCAGAACTTCACTTACTCGTATAACTTCTTCACGGTGAGCCGCCGGCGTGTCTGGGACTGGCGGGCTTTGACGACAAGAGGAATTGCTTGCCGAATAGGCCAGCGCAGCGAACGGCAGCGCAGTGTAAGTCGTTCTTGTCGAAGCCTGTGGATTGTAGTTCTTTTAGATGTAAGCCTTTTGTTCCGTCTTTGCCTACTCCTCCTCCGCCTTCGCCTCCACTTCCTATGCCTTCACCGCCGCCTATTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005198 GO:0005199 GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8

Protein Analysis

436

Amino Acids

49.5

Weight (kDa)

9.38

Isoelectric Point (pI)

44.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	163 - 220	7.1e-07	Leucine rich repeat
LRR_14	PF23598	269 - 357	4.6e-06	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007583)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24480 AT4G13340
fragaria_vesca	FvH4_1g05180 FvH4_1g05180 FvH4_1g05180 FvH4_1g05180
malus_domestica	MD09G1216000.v1.1 MD15G1188400.v1.1
prunus_persica	Prupe.5G056300_v2.0.a1 Prupe.7G228100_v2.0.a1
pyrus_communis	pycom02g04230 pycom15g16890
rosa_chinensis	RchiOBHm_Chr2g0090891
rosa_laevigata	RLG00000016154
rosa_multiflora	Rmu_co8180576.1_g000001 Rmu_sc0000792.1_g000014 Rmu_sc0014837.1_g000001
rosa_roxburghii	Rroxscaffold_2G00150760
rosa_rugosa	Rorug02G0010800
rosa_samantha	Rh2AG057100 Rh2BG055600 Rh2CG057900 Rh2DG056500
rosa_wichuraiana	Rw2G005230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	930
AccB1I	GGYRCC	1 cut(s)	673
AccB7I	CCANNNNNTGG	1 cut(s)	946
AccI	GTMKAC	1 cut(s)	234
AccII	CGCG	3 cut(s)	62, 198, 216
AccIII	TCCGGA	2 cut(s)	267, 408
AclWI	GGATC	5 cut(s)	355, 400, 431, 492, 651
AcsI	RAATTY	2 cut(s)	635, 760
AcuI	CTGAAG	1 cut(s)	926
AcyI	GRCGYC	2 cut(s)	705, 927
AfaI	GTAC	2 cut(s)	365, 455
AfiI	CCNNNNNNNGG	5 cut(s)	244, 415, 610, 896, 946
AflIII	ACRYGT	1 cut(s)	831
AgeI	ACCGGT	1 cut(s)	726
AgsI	TTSAA	5 cut(s)	298, 325, 544, 655, 937
AjuI	GAANNNNNNNTTGG	2 cut(s)	782, 814
AloI	GAACNNNNNNTCC	2 cut(s)	635, 667
Alw21I	GWGCWC	2 cut(s)	99, 570
Alw26I	GTCTC	2 cut(s)	775, 956
AlwI	GGATC	5 cut(s)	355, 400, 431, 492, 651
AlwNI	CAGNNNCTG	2 cut(s)	519, 946
Ama87I	CYCGRG	1 cut(s)	606
Aor13HI	TCCGGA	2 cut(s)	267, 408
AoxI	GGCC	3 cut(s)	303, 1002, 1156
ApoI	RAATTY	2 cut(s)	635, 760
AsiGI	ACCGGT	1 cut(s)	726
AspLEI	GCGC	3 cut(s)	391, 1164, 1179
AspS9I	GGNCC	2 cut(s)	140, 1003
AsuC2I	CCSGG	1 cut(s)	451
AsuHPI	GGTGA	4 cut(s)	221, 991, 1105, 1283
AsuII	TTCGAA	1 cut(s)	663
AvaI	CYCGRG	1 cut(s)	606
AvaII	GGWCC	1 cut(s)	140
BanI	GGYRCC	1 cut(s)	673
BanII	GRGCYC	1 cut(s)	570
BarI	GAAGNNNNNNTAC	2 cut(s)	1070, 1102
BbsI	GAAGAC	2 cut(s)	17, 241
Bbv12I	GWGCWC	2 cut(s)	99, 570
BccI	CCATC	1 cut(s)	970
BceAI	ACGGC	3 cut(s)	454, 820, 1187
BcgI	CGANNNNNNTGC	2 cut(s)	325, 359
BcnI	CCSGG	1 cut(s)	451
BcoDI	GTCTC	2 cut(s)	775, 956
BfaI	CTAG	1 cut(s)	819
BfmI	CTRYAG	2 cut(s)	287, 1058
BfoI	RGCGCY	1 cut(s)	392
BglI	GCCNNNNNGGC	1 cut(s)	1155
Bme1390I	CCNGG	1 cut(s)	451
Bme18I	GGWCC	1 cut(s)	140
BmeT110I	CYCGRG	1 cut(s)	606
BmgT120I	GGNCC	2 cut(s)	140, 1003
BmiI	GGNNCC	2 cut(s)	675, 731
BmrFI	CCNGG	1 cut(s)	451
BmsI	GCATC	1 cut(s)	891
BpiI	GAAGAC	2 cut(s)	17, 241
BpmI	CTGGAG	1 cut(s)	602
Bpu10I	CCTNAGC	1 cut(s)	90
Bpu14I	TTCGAA	1 cut(s)	663
BpuEI	CTTGAG	1 cut(s)	536
BpuMI	CCSGG	1 cut(s)	451
BsaHI	GRCGYC	2 cut(s)	705, 927
BsaI	GGTCTC	1 cut(s)	775
BsaJI	CCNNGG	6 cut(s)	136, 214, 384, 436, 605, 802
BsaWI	WCCGGW	7 cut(s)	267, 368, 408, 726, 732, 873, 958
BsaXI	ACNNNNNCTCC	6 cut(s)	491, 521, 963, 993, 1245, 1275
Bsc4I	CCNNNNNNNGG	5 cut(s)	244, 415, 610, 896, 946
Bse118I	RCCGGY	3 cut(s)	726, 766, 1101
Bse1I	ACTGG	2 cut(s)	940, 1121
BseAI	TCCGGA	2 cut(s)	267, 408
BseDI	CCNNGG	6 cut(s)	136, 214, 384, 436, 605, 802
BseGI	GGATG	2 cut(s)	906, 981
BseLI	CCNNNNNNNGG	5 cut(s)	244, 415, 610, 896, 946
BseMII	CTCAG	2 cut(s)	104, 638
BseNI	ACTGG	2 cut(s)	940, 1121
BseRI	GAGGAG	5 cut(s)	29, 176, 479, 1247, 1250
BsgI	GTGCAG	1 cut(s)	364
Bsh1236I	CGCG	3 cut(s)	62, 198, 216
Bsh1285I	CGRYCG	3 cut(s)	234, 262, 878
BshFI	GGCC	3 cut(s)	305, 1004, 1158
BshNI	GGYRCC	1 cut(s)	673
BshTI	ACCGGT	1 cut(s)	726
BsiEI	CGRYCG	3 cut(s)	234, 262, 878
BsiHKAI	GWGCWC	2 cut(s)	99, 570
BsiHKCI	CYCGRG	1 cut(s)	606
BslFI	GGGAC	2 cut(s)	682, 1127
BslI	CCNNNNNNNGG	5 cut(s)	244, 415, 610, 896, 946
BsmAI	GTCTC	2 cut(s)	775, 956
BsmBI	CGTCTC	1 cut(s)	956
BsmFI	GGGAC	2 cut(s)	682, 1127
BsnI	GGCC	3 cut(s)	305, 1004, 1158
Bso31I	GGTCTC	1 cut(s)	775
BsoBI	CYCGRG	1 cut(s)	606
Bsp119I	TTCGAA	1 cut(s)	663
Bsp1286I	GDGCHC	2 cut(s)	99, 570
Bsp13I	TCCGGA	2 cut(s)	267, 408
Bsp143I	GATC	6 cut(s)	347, 405, 423, 484, 643, 739
Bsp19I	CCATGG	1 cut(s)	136
BspANI	GGCC	3 cut(s)	305, 1004, 1158
BspCNI	CTCAG	2 cut(s)	103, 639
BspEI	TCCGGA	2 cut(s)	267, 408
BspFNI	CGCG	3 cut(s)	62, 198, 216
BspLI	GGNNCC	2 cut(s)	675, 731
BspMAI	CTGCAG	2 cut(s)	291, 1062
BspPI	GGATC	5 cut(s)	355, 400, 431, 492, 651
BspT104I	TTCGAA	1 cut(s)	663
BspT107I	GGYRCC	1 cut(s)	673
BspTNI	GGTCTC	1 cut(s)	775
BsrFI	RCCGGY	3 cut(s)	726, 766, 1101
BsrI	ACTGG	2 cut(s)	940, 1121
BssAI	RCCGGY	3 cut(s)	726, 766, 1101
BssECI	CCNNGG	6 cut(s)	136, 214, 384, 436, 605, 802
BssMI	GATC	6 cut(s)	347, 405, 423, 484, 643, 739
BssNI	GRCGYC	2 cut(s)	705, 927
BssT1I	CCWWGG	2 cut(s)	136, 384
Bst4CI	ACNGT	6 cut(s)	232, 415, 529, 673, 919, 1093
Bst6I	CTCTTC	2 cut(s)	29, 77
BstACI	GRCGYC	2 cut(s)	705, 927
BstBI	TTCGAA	1 cut(s)	663
BstC8I	GCNNGC	8 cut(s)	182, 221, 291, 303, 1103, 1122, 1147, 1160
BstDEI	CTNAG	2 cut(s)	90, 647
BstDSI	CCRYGG	4 cut(s)	136, 214, 436, 802
BstEII	GGTNACC	1 cut(s)	209
BstF5I	GGATG	2 cut(s)	906, 981
BstFNI	CGCG	3 cut(s)	62, 198, 216
BstH2I	RGCGCY	1 cut(s)	392
BstHHI	GCGC	3 cut(s)	391, 1164, 1179
BstKTI	GATC	6 cut(s)	350, 408, 426, 487, 646, 742
BstMAI	GTCTC	2 cut(s)	775, 956
BstMBI	GATC	6 cut(s)	347, 405, 423, 484, 643, 739
BstMCI	CGRYCG	3 cut(s)	234, 262, 878
BstNSI	RCATGY	2 cut(s)	227, 835
BstPI	GGTNACC	1 cut(s)	209
BstSCI	CCNGG	1 cut(s)	449
BstSFI	CTRYAG	2 cut(s)	287, 1058
BstUI	CGCG	3 cut(s)	62, 198, 216
BstV2I	GAAGAC	2 cut(s)	17, 241
BstX2I	RGATCY	3 cut(s)	347, 484, 643
BstYI	RGATCY	3 cut(s)	347, 484, 643
BsuRI	GGCC	3 cut(s)	305, 1004, 1158
BtgI	CCRYGG	4 cut(s)	136, 214, 436, 802
BtgZI	GCGATG	1 cut(s)	213
BtsCI	GGATG	2 cut(s)	906, 981
BtsI	GCAGTG	2 cut(s)	42, 1186
BtsIMutI	CAGTG	2 cut(s)	42, 1186
Cac8I	GCNNGC	8 cut(s)	182, 221, 291, 303, 1103, 1122, 1147, 1160
CaiI	CAGNNNCTG	2 cut(s)	519, 946
CfoI	GCGC	3 cut(s)	391, 1164, 1179
Cfr10I	RCCGGY	3 cut(s)	726, 766, 1101
Cfr13I	GGNCC	2 cut(s)	140, 1003
Cfr42I	CCGCGG	1 cut(s)	217
CseI	GACGC	1 cut(s)	713
Csp6I	GTAC	2 cut(s)	364, 454
CspAI	ACCGGT	1 cut(s)	726
CviAII	CATG	3 cut(s)	137, 224, 832
CviQI	GTAC	2 cut(s)	364, 454
DdeI	CTNAG	2 cut(s)	90, 647
DpnI	GATC	6 cut(s)	349, 407, 425, 486, 645, 741
DpnII	GATC	6 cut(s)	347, 405, 423, 484, 643, 739
Eam1104I	CTCTTC	2 cut(s)	29, 77
EarI	CTCTTC	2 cut(s)	29, 77
EciI	GGCGGA	1 cut(s)	1253
Ecl136II	GAGCTC	1 cut(s)	568
Eco130I	CCWWGG	2 cut(s)	136, 384
Eco24I	GRGCYC	1 cut(s)	570
Eco31I	GGTCTC	1 cut(s)	775
Eco47I	GGWCC	1 cut(s)	140
Eco53kI	GAGCTC	1 cut(s)	568
Eco57I	CTGAAG	1 cut(s)	926
Eco88I	CYCGRG	1 cut(s)	606
Eco91I	GGTNACC	1 cut(s)	209
EcoICRI	GAGCTC	1 cut(s)	568
EcoO65I	GGTNACC	1 cut(s)	209
EcoRI	GAATTC	1 cut(s)	760
EcoT14I	CCWWGG	2 cut(s)	136, 384
EcoT38I	GRGCYC	1 cut(s)	570
ErhI	CCWWGG	2 cut(s)	136, 384
Esp3I	CGTCTC	1 cut(s)	956
FaeI	CATG	3 cut(s)	140, 227, 835
FaiI	YATR	7 cut(s)	138, 225, 281, 446, 833, 1080, 1285
FalI	AAGNNNNNCTT	2 cut(s)	1177, 1209
FaqI	GGGAC	2 cut(s)	682, 1127
FatI	CATG	3 cut(s)	136, 223, 831
FauI	CCCGC	2 cut(s)	412, 1113
FblI	GTMKAC	1 cut(s)	234
FokI	GGATG	2 cut(s)	913, 988
FriOI	GRGCYC	1 cut(s)	570
FspBI	CTAG	1 cut(s)	819
GlaI	GCGC	3 cut(s)	390, 1163, 1178
GsuI	CTGGAG	1 cut(s)	602
HaeII	RGCGCY	1 cut(s)	392
HaeIII	GGCC	3 cut(s)	305, 1004, 1158
HgaI	GACGC	1 cut(s)	713
HhaI	GCGC	3 cut(s)	391, 1164, 1179
Hin1I	GRCGYC	2 cut(s)	705, 927
Hin1II	CATG	3 cut(s)	140, 227, 835
Hin6I	GCGC	3 cut(s)	389, 1162, 1177
HinP1I	GCGC	3 cut(s)	389, 1162, 1177
HincII	GTYRAC	1 cut(s)	235
HindII	GTYRAC	1 cut(s)	235
HinfI	GANTC	3 cut(s)	870, 1027, 1054
HphI	GGTGA	4 cut(s)	221, 991, 1105, 1283
Hpy166II	GTNNAC	2 cut(s)	235, 366
Hpy188I	TCNGA	4 cut(s)	151, 316, 347, 648
Hpy188III	TCNNGA	6 cut(s)	92, 253, 268, 409, 757, 856
Hpy8I	GTNNAC	2 cut(s)	235, 366
Hpy99I	CGWCG	1 cut(s)	155
HpyAV	CCTTC	3 cut(s)	659, 1277, 1298
HpyCH4III	ACNGT	6 cut(s)	232, 415, 529, 673, 919, 1093
HpyCH4IV	ACGT	1 cut(s)	927
HpyCH4V	TGCA	4 cut(s)	289, 360, 381, 1060
HpyF3I	CTNAG	2 cut(s)	90, 647
HpySE526I	ACGT	1 cut(s)	927
Hsp92I	GRCGYC	2 cut(s)	705, 927
Hsp92II	CATG	3 cut(s)	140, 227, 835
HspAI	GCGC	3 cut(s)	389, 1162, 1177
Kpn2I	TCCGGA	2 cut(s)	267, 408
KroI	GCCGGC	1 cut(s)	1101
KroNI	GCCGGC	1 cut(s)	1103
KspI	CCGCGG	1 cut(s)	217
Kzo9I	GATC	6 cut(s)	347, 405, 423, 484, 643, 739
LmnI	GCTCC	5 cut(s)	94, 189, 466, 682, 988
LweI	GCATC	1 cut(s)	891
MaeI	CTAG	1 cut(s)	819
MaeII	ACGT	1 cut(s)	927
MaeIII	GTNAC	2 cut(s)	209, 226
MalI	GATC	6 cut(s)	349, 407, 425, 486, 645, 741
MboI	GATC	6 cut(s)	347, 405, 423, 484, 643, 739
MboII	GAAGA	9 cut(s)	16, 16, 19, 22, 64, 241, 919, 1036, 1078
MfeI	CAATTG	1 cut(s)	337
MflI	RGATCY	3 cut(s)	347, 484, 643
MhlI	GDGCHC	2 cut(s)	99, 570
MluCI	AATT	4 cut(s)	337, 635, 760, 1140
MlyI	GAGTC	1 cut(s)	1063
MmeI	TCCRAC	4 cut(s)	174, 325, 339, 534
MreI	CGCCGGCG	1 cut(s)	1101
MroI	TCCGGA	2 cut(s)	267, 408
MroNI	GCCGGC	1 cut(s)	1101
MspA1I	CMGCKG	2 cut(s)	216, 622
MspR9I	CCNGG	1 cut(s)	451
MunI	CAATTG	1 cut(s)	337
MvnI	CGCG	3 cut(s)	62, 198, 216
NaeI	GCCGGC	1 cut(s)	1103
NciI	CCSGG	1 cut(s)	451
NcoI	CCATGG	1 cut(s)	136
NdeII	GATC	6 cut(s)	347, 405, 423, 484, 643, 739
NgoMIV	GCCGGC	1 cut(s)	1101
NlaIII	CATG	3 cut(s)	140, 227, 835
NlaIV	GGNNCC	2 cut(s)	675, 731
NmeAIII	GCCGAG	1 cut(s)	1077
NmuCI	GTSAC	2 cut(s)	209, 226
NspI	RCATGY	2 cut(s)	227, 835
NspV	TTCGAA	1 cut(s)	663
PciI	ACATGT	1 cut(s)	831
PcsI	WCGNNNNNNNCGW	1 cut(s)	1195
PdiI	GCCGGC	1 cut(s)	1103
PfeI	GAWTC	2 cut(s)	870, 1027
PflMI	CCANNNNNTGG	1 cut(s)	946
PinAI	ACCGGT	1 cut(s)	726
PleI	GAGTC	1 cut(s)	1062
PpsI	GAGTC	1 cut(s)	1062
PscI	ACATGT	1 cut(s)	831
Psp124BI	GAGCTC	1 cut(s)	570
PspEI	GGTNACC	1 cut(s)	209
PspN4I	GGNNCC	2 cut(s)	675, 731
PspPI	GGNCC	2 cut(s)	140, 1003
PsrI	GAACNNNNNNTAC	2 cut(s)	1055, 1087
PstI	CTGCAG	2 cut(s)	291, 1062
PstNI	CAGNNNCTG	2 cut(s)	519, 946
PsuI	RGATCY	3 cut(s)	347, 484, 643
PvuII	CAGCTG	1 cut(s)	622
RsaI	GTAC	2 cut(s)	365, 455
RsaNI	GTAC	2 cut(s)	364, 454
SacI	GAGCTC	1 cut(s)	570
SacII	CCGCGG	1 cut(s)	217
SalI	GTCGAC	1 cut(s)	233
Sau3AI	GATC	6 cut(s)	347, 405, 423, 484, 643, 739
Sau96I	GGNCC	2 cut(s)	140, 1003
SchI	GAGTC	1 cut(s)	1063
ScrFI	CCNGG	1 cut(s)	451
SduI	GDGCHC	2 cut(s)	99, 570
SfaNI	GCATC	1 cut(s)	891
SfcI	CTRYAG	2 cut(s)	287, 1058
Sfr303I	CCGCGG	1 cut(s)	217
SfuI	TTCGAA	1 cut(s)	663
SgrAI	CRCCGGYG	1 cut(s)	1101
SgrBI	CCGCGG	1 cut(s)	217
SinI	GGWCC	1 cut(s)	140
SmlI	CTYRAG	1 cut(s)	551
SmoI	CTYRAG	1 cut(s)	551
Sse9I	AATT	4 cut(s)	337, 635, 760, 1140
SspMI	CTAG	1 cut(s)	819
SstI	GAGCTC	1 cut(s)	570
StyD4I	CCNGG	1 cut(s)	449
StyI	CCWWGG	2 cut(s)	136, 384
TaaI	ACNGT	6 cut(s)	232, 415, 529, 673, 919, 1093
TaiI	ACGT	1 cut(s)	930
TaqI	TCGA	9 cut(s)	234, 252, 426, 564, 570, 663, 690, 738, 1198
TasI	AATT	4 cut(s)	337, 635, 760, 1140
TatI	WGTACW	1 cut(s)	363
TauI	GCSGC	6 cut(s)	62, 117, 219, 889, 1101, 1299
TfiI	GAWTC	2 cut(s)	870, 1027
TscAI	CASTG	2 cut(s)	49, 1186
TseFI	GTSAC	2 cut(s)	209, 226
Tsp45I	GTSAC	2 cut(s)	209, 226
TspDTI	ATGAA	3 cut(s)	17, 703, 875
TspGWI	ACGGA	3 cut(s)	497, 763, 1232
TspRI	CASTG	2 cut(s)	49, 1186
Van91I	CCANNNNNTGG	1 cut(s)	946
VpaK11BI	GGWCC	1 cut(s)	140
XapI	RAATTY	2 cut(s)	635, 760
XceI	RCATGY	2 cut(s)	227, 835
XmiI	GTMKAC	1 cut(s)	234
XspI	CTAG	1 cut(s)	819
ZraI	GACGTC	1 cut(s)	928

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.