MD09G1220700.v1.1

Biotin carboxyl carrier protein of acetyl-CoA carboxylase 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009300
Physical Location & Seq
Reverse (-)
25235152 .. 25239371
4220 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1220700.v1.1.491

Sequence Viewer

Length: 822 bp
ATGGCTTCCAGTAGCCTAGGGACATTGAATGCTAAAATCACAAACTTGAATTTCGGTAGAGCAAGAGTTGGGGTTGTAAAGTCATATGGCGTAAGAAGTTGGACGGGGAGAAAACCACAATTGTACTCATGTTTATCGATATCAAGACAGCCAGAGAAAGTATTACATGTGCGCAGCATTCCATCACTAGAAACCCTGTCTGCTACAAGTTTAGAAGAGGTTCCTGAAGAGAGTGGAGATTCTGGTCCGACAAACCAGCTCCTTCAAAATTTTGACAAGATACAATCTTTGCTCACAGCAATATGTGATACAACTACAGTTGCAGAGGTCAAAGTAAAAATCGGTGGATTTCGGTTAAATGTGGTGAGGCAGCTGACTGAAAAAATTAGCACACCACCTCCCCCAAGTCCTGTTCCTGTTAGTGCTAGTGAAAATGCTAAAGCACTTGATTCGAATGGCACTGTTCCTACACAATCTGTAGCTATCACTAGACAAGAATCGTCTTCGAGGAGTATCCAGACTTTGCTAAATAGAGCGGCAGATGATGGCTTGGTGTTAATTCGTTCTCCACGAGTGGGGTTGTTTAGGAGATCTCGAACCATAAAGGGGAAGCGTGCTCCACCATCATGTAAAGAGAAGCAAACCGTGAAGGAGGGACAAGTGATTTGCTACATAGAACAGCTTGGTGGGGAGCTCCCGATTGAGTCTGATGTAGCTGGGGAGGTTATCAGGATACTTCGAGAAGATGGCGATCCCGTCGGATATGGTGATGCTCTTGTTGCAGTTCTCCCCTCATTTCCCGGGATTAAGAGACTTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Biotin_lipoyl

Protein Analysis

274

Amino Acids

29.43

Weight (kDa)

9.43

Isoelectric Point (pI)

61.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_lipoyl PF00364 205 - 261 3.4e-08 Biotin-requiring enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 173
AccBSI CCGCTC 1 cut(s) 536
AciI CCGC 1 cut(s) 536
AclWI GGATC 1 cut(s) 746
AcsI RAATTY 2 cut(s) 49, 268
AcuI CTGAAG 1 cut(s) 246
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 2 cut(s) 575, 606
AflIII ACRYGT 1 cut(s) 166
AgsI TTSAA 4 cut(s) 28, 49, 266, 818
AluBI AGCT 6 cut(s) 259, 373, 482, 682, 694, 716
AluI AGCT 6 cut(s) 259, 373, 482, 682, 694, 716
Alw21I GWGCWC 2 cut(s) 619, 696
Alw26I GTCTC 1 cut(s) 805
AlwI GGATC 1 cut(s) 746
Ama87I CYCGRG 1 cut(s) 800
ApeKI GCWGC 2 cut(s) 174, 370
ApoI RAATTY 2 cut(s) 49, 268
ArsI GACNNNNNNTTYG 2 cut(s) 648, 680
Asp700I GAANNNNTTC 1 cut(s) 219
AspA2I CCTAGG 1 cut(s) 16
AspLEI GCGC 1 cut(s) 174
AspS9I GGNCC 1 cut(s) 245
AsuC2I CCSGG 2 cut(s) 801, 802
AsuHPI GGTGA 2 cut(s) 376, 779
AsuII TTCGAA 1 cut(s) 452
AvaI CYCGRG 1 cut(s) 800
AvaII GGWCC 1 cut(s) 245
AvrII CCTAGG 1 cut(s) 16
BanII GRGCYC 1 cut(s) 696
BauI CACGAG 1 cut(s) 570
BbsI GAAGAC 1 cut(s) 495
Bbv12I GWGCWC 2 cut(s) 619, 696
BbvI GCAGC 2 cut(s) 186, 382
BccI CCATC 4 cut(s) 190, 539, 631, 740
BcgI CGANNNNNNTGC 2 cut(s) 432, 466
BciVI GTATCC 2 cut(s) 524, 726
BcnI CCSGG 2 cut(s) 801, 802
BcoDI GTCTC 1 cut(s) 805
BfaI CTAG 4 cut(s) 17, 188, 426, 489
BfmI CTRYAG 2 cut(s) 315, 477
BfuI GTATCC 2 cut(s) 524, 726
BglII AGATCT 1 cut(s) 590
BisI GCNGC 3 cut(s) 175, 371, 537
BlnI CCTAGG 1 cut(s) 16
BlsI GCNGC 3 cut(s) 176, 372, 538
Bme1390I CCNGG 2 cut(s) 801, 802
Bme18I GGWCC 1 cut(s) 245
BmeT110I CYCGRG 1 cut(s) 800
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 1 cut(s) 222
BmrFI CCNGG 2 cut(s) 801, 802
BmsI GCATC 1 cut(s) 760
BpiI GAAGAC 1 cut(s) 495
Bpu14I TTCGAA 1 cut(s) 452
BpuMI CCSGG 2 cut(s) 801, 802
Bsa29I ATCGAT 1 cut(s) 137
BsaBI GATNNNNATC 1 cut(s) 750
BsaJI CCNNGG 2 cut(s) 16, 800
BsaXI ACNNNNNCTCC 2 cut(s) 382, 412
Bsc4I CCNNNNNNNGG 2 cut(s) 575, 606
Bse1I ACTGG 1 cut(s) 9
Bse8I GATNNNNATC 1 cut(s) 750
BseCI ATCGAT 1 cut(s) 137
BseDI CCNNGG 2 cut(s) 16, 800
BseJI GATNNNNATC 1 cut(s) 750
BseLI CCNNNNNNNGG 2 cut(s) 575, 606
BseNI ACTGG 1 cut(s) 9
BseRI GAGGAG 1 cut(s) 523
BseXI GCAGC 2 cut(s) 186, 382
BseYI CCCAGC 1 cut(s) 716
BshVI ATCGAT 1 cut(s) 137
BsiHKAI GWGCWC 2 cut(s) 619, 696
BsiHKCI CYCGRG 1 cut(s) 800
BsiSI CCGG 1 cut(s) 801
BslFI GGGAC 2 cut(s) 34, 669
BslI CCNNNNNNNGG 2 cut(s) 575, 606
BsmAI GTCTC 1 cut(s) 805
BsmFI GGGAC 2 cut(s) 34, 669
BsmI GAATGC 2 cut(s) 34, 177
BsoBI CYCGRG 1 cut(s) 800
Bsp119I TTCGAA 1 cut(s) 452
Bsp1286I GDGCHC 2 cut(s) 619, 696
Bsp143I GATC 2 cut(s) 590, 751
BspACI CCGC 1 cut(s) 536
BspDI ATCGAT 1 cut(s) 137
BspLI GGNNCC 1 cut(s) 222
BspPI GGATC 1 cut(s) 746
BspT104I TTCGAA 1 cut(s) 452
BsrBI CCGCTC 1 cut(s) 536
BsrI ACTGG 1 cut(s) 9
BssECI CCNNGG 2 cut(s) 16, 800
BssMI GATC 2 cut(s) 590, 751
BssSI CACGAG 1 cut(s) 570
BssT1I CCWWGG 1 cut(s) 16
Bst2BI CACGAG 1 cut(s) 570
Bst4CI ACNGT 3 cut(s) 319, 463, 646
Bst6I CTCTTC 2 cut(s) 210, 222
BstBI TTCGAA 1 cut(s) 452
BstC8I GCNNGC 1 cut(s) 615
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 2 cut(s) 593, 754
BstMAI GTCTC 1 cut(s) 805
BstMBI GATC 2 cut(s) 590, 751
BstMWI GCNNNNNNNGC 1 cut(s) 779
BstNSI RCATGY 1 cut(s) 170
BstSCI CCNGG 2 cut(s) 799, 800
BstSFI CTRYAG 2 cut(s) 315, 477
BstV1I GCAGC 2 cut(s) 186, 382
BstV2I GAAGAC 1 cut(s) 495
BstX2I RGATCY 1 cut(s) 590
BstYI RGATCY 1 cut(s) 590
Bsu15I ATCGAT 1 cut(s) 137
BsuI GTATCC 2 cut(s) 524, 726
BsuTUI ATCGAT 1 cut(s) 137
BtsIMutI CAGTG 1 cut(s) 459
Cac8I GCNNGC 1 cut(s) 615
CfoI GCGC 1 cut(s) 174
Cfr13I GGNCC 1 cut(s) 245
Cfr9I CCCGGG 1 cut(s) 800
ClaI ATCGAT 1 cut(s) 137
Csp6I GTAC 1 cut(s) 124
CviAII CATG 3 cut(s) 129, 167, 627
CviQI GTAC 1 cut(s) 124
DpnI GATC 2 cut(s) 592, 753
DpnII GATC 2 cut(s) 590, 751
Eam1104I CTCTTC 2 cut(s) 210, 222
EarI CTCTTC 2 cut(s) 210, 222
Ecl136II GAGCTC 1 cut(s) 694
Eco130I CCWWGG 1 cut(s) 16
Eco24I GRGCYC 1 cut(s) 696
Eco32I GATATC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 245
Eco53kI GAGCTC 1 cut(s) 694
Eco57I CTGAAG 1 cut(s) 246
Eco88I CYCGRG 1 cut(s) 800
EcoICRI GAGCTC 1 cut(s) 694
EcoRV GATATC 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 16
EcoT38I GRGCYC 1 cut(s) 696
ErhI CCWWGG 1 cut(s) 16
FaeI CATG 3 cut(s) 132, 170, 630
FaiI YATR 9 cut(s) 85, 87, 130, 168, 304, 602, 628, 674, 765
FaqI GGGAC 2 cut(s) 34, 669
FatI CATG 3 cut(s) 128, 166, 626
FauNDI CATATG 1 cut(s) 85
Fnu4HI GCNGC 3 cut(s) 175, 371, 537
FriOI GRGCYC 1 cut(s) 696
Fsp4HI GCNGC 3 cut(s) 175, 371, 537
FspBI CTAG 4 cut(s) 17, 188, 426, 489
FspI TGCGCA 1 cut(s) 173
GlaI GCGC 1 cut(s) 173
GluI GCNGC 3 cut(s) 175, 371, 537
GsaI CCCAGC 1 cut(s) 720
HapII CCGG 1 cut(s) 801
HhaI GCGC 1 cut(s) 174
Hin1II CATG 3 cut(s) 132, 170, 630
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HinfI GANTC 4 cut(s) 239, 449, 497, 704
HpaII CCGG 1 cut(s) 801
HphI GGTGA 2 cut(s) 376, 779
Hpy188I TCNGA 3 cut(s) 249, 709, 761
Hpy188III TCNNGA 7 cut(s) 144, 224, 517, 594, 697, 730, 740
Hpy99I CGWCG 1 cut(s) 761
HpyAV CCTTC 2 cut(s) 272, 643
HpyCH4III ACNGT 3 cut(s) 319, 463, 646
HpyCH4V TGCA 2 cut(s) 323, 782
HpyF10VI GCNNNNNNNGC 1 cut(s) 779
Hsp92II CATG 3 cut(s) 132, 170, 630
HspAI GCGC 1 cut(s) 172
Kzo9I GATC 2 cut(s) 590, 751
LmnI GCTCC 4 cut(s) 264, 622, 691, 699
Lsp1109I GCAGC 2 cut(s) 186, 382
LweI GCATC 1 cut(s) 760
MaeI CTAG 4 cut(s) 17, 188, 426, 489
MalI GATC 2 cut(s) 592, 753
MbiI CCGCTC 1 cut(s) 536
MboI GATC 2 cut(s) 590, 751
MboII GAAGA 4 cut(s) 227, 239, 495, 755
MfeI CAATTG 1 cut(s) 119
MflI RGATCY 1 cut(s) 590
MhlI GDGCHC 2 cut(s) 619, 696
MluCI AATT 5 cut(s) 49, 119, 268, 384, 558
MlyI GAGTC 1 cut(s) 713
MmeI TCCRAC 3 cut(s) 80, 272, 739
MnlI CCTC 8 cut(s) 211, 319, 360, 408, 501, 646, 715, 802
MroXI GAANNNNTTC 1 cut(s) 219
MseI TTAA 3 cut(s) 356, 557, 807
MslI CAYNNNNRTG 1 cut(s) 625
MspA1I CMGCKG 1 cut(s) 373
MspI CCGG 1 cut(s) 801
MspR9I CCNGG 2 cut(s) 801, 802
MunI CAATTG 1 cut(s) 119
Mva1269I GAATGC 2 cut(s) 34, 177
MwoI GCNNNNNNNGC 1 cut(s) 779
NciI CCSGG 2 cut(s) 801, 802
NdeI CATATG 1 cut(s) 85
NdeII GATC 2 cut(s) 590, 751
NlaIII CATG 3 cut(s) 132, 170, 630
NlaIV GGNNCC 1 cut(s) 222
NsbI TGCGCA 1 cut(s) 173
NspI RCATGY 1 cut(s) 170
NspV TTCGAA 1 cut(s) 452
PciI ACATGT 1 cut(s) 166
PcsI WCGNNNNNNNCGW 1 cut(s) 568
PctI GAATGC 2 cut(s) 34, 177
PdmI GAANNNNTTC 1 cut(s) 219
PfeI GAWTC 3 cut(s) 239, 449, 497
PkrI GCNGC 3 cut(s) 176, 372, 538
PleI GAGTC 1 cut(s) 712
PpsI GAGTC 1 cut(s) 712
PscI ACATGT 1 cut(s) 166
Psp124BI GAGCTC 1 cut(s) 696
PspFI CCCAGC 1 cut(s) 716
PspN4I GGNNCC 1 cut(s) 222
PspPI GGNCC 1 cut(s) 245
PsuI RGATCY 1 cut(s) 590
PvuII CAGCTG 1 cut(s) 373
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
RseI CAYNNNNRTG 1 cut(s) 625
SacI GAGCTC 1 cut(s) 696
SaqAI TTAA 3 cut(s) 356, 557, 807
SatI GCNGC 3 cut(s) 175, 371, 537
Sau3AI GATC 2 cut(s) 590, 751
Sau96I GGNCC 1 cut(s) 245
SchI GAGTC 1 cut(s) 713
ScrFI CCNGG 2 cut(s) 801, 802
SduI GDGCHC 2 cut(s) 619, 696
SfaNI GCATC 1 cut(s) 760
SfcI CTRYAG 2 cut(s) 315, 477
SfuI TTCGAA 1 cut(s) 452
SinI GGWCC 1 cut(s) 245
SmaI CCCGGG 1 cut(s) 802
SmiMI CAYNNNNRTG 1 cut(s) 625
Sse9I AATT 5 cut(s) 49, 119, 268, 384, 558
SsiI CCGC 1 cut(s) 536
SspMI CTAG 4 cut(s) 17, 188, 426, 489
SstI GAGCTC 1 cut(s) 696
StyD4I CCNGG 2 cut(s) 799, 800
StyI CCWWGG 1 cut(s) 16
TaaI ACNGT 3 cut(s) 319, 463, 646
TaqI TCGA 5 cut(s) 137, 452, 506, 595, 739
TasI AATT 5 cut(s) 49, 119, 268, 384, 558
TatI WGTACW 1 cut(s) 123
TauI GCSGC 1 cut(s) 539
TfiI GAWTC 3 cut(s) 239, 449, 497
Tru1I TTAA 3 cut(s) 356, 557, 807
Tru9I TTAA 3 cut(s) 356, 557, 807
TscAI CASTG 1 cut(s) 466
TseI GCWGC 2 cut(s) 174, 370
TspMI CCCGGG 1 cut(s) 800
TspRI CASTG 1 cut(s) 466
VpaK11BI GGWCC 1 cut(s) 245
XapI RAATTY 2 cut(s) 49, 268
XceI RCATGY 1 cut(s) 170
XmaI CCCGGG 1 cut(s) 800
XmaJI CCTAGG 1 cut(s) 16
XmnI GAANNNNTTC 1 cut(s) 219
XspI CTAG 4 cut(s) 17, 188, 426, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.