MD17G1204600.v1.1

Biotin carboxyl carrier protein of acetyl-CoA carboxylase 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009300
Physical Location & Seq
Forward (+)
24773246 .. 24777901
4656 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1204600.v1.1.491

Sequence Viewer

Length: 732 bp
ATGGCTTCCAGTAGCCTAGGGACATTGAATGCTAAAATTACGAACTTGAACTTCGGTAGAGCACGGGTTGGGGTTTTACAGTCATATGGTGTAAGAACTTGGACAGGGAGAAAACCACAGTTGTACTCATGTTTATCGATATCAAGACAGTCATCAGAAAAAGTATTACATGAGCACAGCATTCCATCGTTACAAATCCTGTCTGCTAAAAGTTTAGAAGAGGTTTCTGAAGAGAGTGGAGATTCTGGTCCGACAAACCAGCTCGTTCAAAATTTTGATGAGGTACAATCTTTGCTCACAGCAATATGTGATACAACTACAGTTGCAGAGGTCAAATTAAAAATTGGTGGATTTCGGTTAAATGTGGTGAGGAAGTTGACTGAAAAAATTTGCACACCACCTCCCCCAAGTCCTGCTGCTGTTAGTGCGAGTGAAAATGCTAAAGCACTTGATTCGAATGGTGTTGTTCCTACACAATCTGTAGCTATCACTAGACAAGAGAGATCTCGAACCATAAAGGGGAAGCGTGCTCCGCCATCATGTAAAGAGCAAATAGTGAAGGAGGGACAAGTGATTTGCTACATAGAACAGCTTGGCGGGGAGCTCCCAATTGAGTCTGATGTAGCTGGGGAGGTCATCAGGATACTACAAGAAGATGGTGATCCCGTTGGATATGGTGATGCTCTCGTTGCGGTTCTCCCCTCATTTCCTGGGATTAAGAAACTTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Biotin_lipoyl

Protein Analysis

244

Amino Acids

26.07

Weight (kDa)

7.6

Isoelectric Point (pI)

56.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_lipoyl PF00364 186 - 231 8.8e-07 Biotin-requiring enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 533, 597, 692
AclWI GGATC 1 cut(s) 656
AcsI RAATTY 2 cut(s) 271, 387
AcuI CTGAAG 1 cut(s) 249
AfaI GTAC 2 cut(s) 125, 285
AfiI CCNNNNNNNGG 1 cut(s) 519
AgsI TTSAA 4 cut(s) 28, 49, 269, 728
AjnI CCWGG 1 cut(s) 709
AluBI AGCT 5 cut(s) 262, 485, 592, 604, 626
AluI AGCT 5 cut(s) 262, 485, 592, 604, 626
Alw21I GWGCWC 4 cut(s) 64, 177, 532, 606
AlwI GGATC 1 cut(s) 656
ApeKI GCWGC 1 cut(s) 416
ApoI RAATTY 2 cut(s) 271, 387
ArsI GACNNNNNNTTYG 2 cut(s) 558, 590
AspA2I CCTAGG 1 cut(s) 16
AspS9I GGNCC 1 cut(s) 248
AsuHPI GGTGA 3 cut(s) 379, 671, 689
AsuII TTCGAA 1 cut(s) 455
AvaII GGWCC 1 cut(s) 248
AvrII CCTAGG 1 cut(s) 16
BanII GRGCYC 1 cut(s) 606
Bbv12I GWGCWC 4 cut(s) 64, 177, 532, 606
BbvI GCAGC 1 cut(s) 403
BccI CCATC 3 cut(s) 193, 544, 650
BcgI CGANNNNNNTGC 2 cut(s) 435, 469
BciT130I CCWGG 1 cut(s) 711
BciVI GTATCC 1 cut(s) 636
BfaI CTAG 2 cut(s) 17, 492
BfmI CTRYAG 2 cut(s) 318, 480
BfuI GTATCC 1 cut(s) 636
BglII AGATCT 1 cut(s) 503
BisI GCNGC 1 cut(s) 417
BlnI CCTAGG 1 cut(s) 16
BlsI GCNGC 1 cut(s) 418
Bme1390I CCNGG 1 cut(s) 711
Bme18I GGWCC 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 248
BmrFI CCNGG 1 cut(s) 711
BmsI GCATC 1 cut(s) 670
Bpu14I TTCGAA 1 cut(s) 455
Bsa29I ATCGAT 1 cut(s) 137
BsaBI GATNNNNATC 1 cut(s) 660
BsaJI CCNNGG 2 cut(s) 16, 710
BsaXI ACNNNNNCTCC 2 cut(s) 385, 415
Bsc4I CCNNNNNNNGG 1 cut(s) 519
Bse1I ACTGG 1 cut(s) 9
Bse8I GATNNNNATC 1 cut(s) 660
BseBI CCWGG 1 cut(s) 711
BseCI ATCGAT 1 cut(s) 137
BseDI CCNNGG 2 cut(s) 16, 710
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 1 cut(s) 519
BseNI ACTGG 1 cut(s) 9
BseXI GCAGC 1 cut(s) 403
BseYI CCCAGC 1 cut(s) 626
BshVI ATCGAT 1 cut(s) 137
BsiHKAI GWGCWC 4 cut(s) 64, 177, 532, 606
BslFI GGGAC 2 cut(s) 34, 579
BslI CCNNNNNNNGG 1 cut(s) 519
BsmFI GGGAC 2 cut(s) 34, 579
BsmI GAATGC 2 cut(s) 34, 180
Bsp119I TTCGAA 1 cut(s) 455
Bsp1286I GDGCHC 4 cut(s) 64, 177, 532, 606
Bsp143I GATC 2 cut(s) 503, 661
BspACI CCGC 3 cut(s) 533, 597, 692
BspDI ATCGAT 1 cut(s) 137
BspPI GGATC 1 cut(s) 656
BspT104I TTCGAA 1 cut(s) 455
BsrI ACTGG 1 cut(s) 9
BssECI CCNNGG 2 cut(s) 16, 710
BssMI GATC 2 cut(s) 503, 661
BssT1I CCWWGG 1 cut(s) 16
Bst2UI CCWGG 1 cut(s) 711
Bst4CI ACNGT 4 cut(s) 81, 120, 150, 322
Bst6I CTCTTC 2 cut(s) 213, 225
BstBI TTCGAA 1 cut(s) 455
BstC8I GCNNGC 1 cut(s) 528
BstKTI GATC 2 cut(s) 506, 664
BstMBI GATC 2 cut(s) 503, 661
BstMWI GCNNNNNNNGC 3 cut(s) 425, 532, 689
BstNI CCWGG 1 cut(s) 711
BstSCI CCNGG 1 cut(s) 709
BstSFI CTRYAG 2 cut(s) 318, 480
BstV1I GCAGC 1 cut(s) 403
BstX2I RGATCY 1 cut(s) 503
BstYI RGATCY 1 cut(s) 503
Bsu15I ATCGAT 1 cut(s) 137
BsuI GTATCC 1 cut(s) 636
BsuTUI ATCGAT 1 cut(s) 137
Cac8I GCNNGC 1 cut(s) 528
Cfr13I GGNCC 1 cut(s) 248
ClaI ATCGAT 1 cut(s) 137
Csp6I GTAC 2 cut(s) 124, 284
CviAII CATG 3 cut(s) 129, 170, 540
CviJI RGCY 7 cut(s) 5, 15, 262, 485, 592, 604, 626
CviKI_1 RGCY 7 cut(s) 5, 15, 262, 485, 592, 604, 626
CviQI GTAC 2 cut(s) 124, 284
DpnI GATC 2 cut(s) 505, 663
DpnII GATC 2 cut(s) 503, 661
Eam1104I CTCTTC 2 cut(s) 213, 225
EarI CTCTTC 2 cut(s) 213, 225
EciI GGCGGA 1 cut(s) 522
Ecl136II GAGCTC 1 cut(s) 604
Eco130I CCWWGG 1 cut(s) 16
Eco24I GRGCYC 1 cut(s) 606
Eco32I GATATC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 248
Eco53kI GAGCTC 1 cut(s) 604
Eco57I CTGAAG 1 cut(s) 249
EcoICRI GAGCTC 1 cut(s) 604
EcoRII CCWGG 1 cut(s) 709
EcoRV GATATC 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 16
EcoT38I GRGCYC 1 cut(s) 606
ErhI CCWWGG 1 cut(s) 16
FaeI CATG 3 cut(s) 132, 173, 543
FaiI YATR 9 cut(s) 85, 87, 130, 171, 307, 515, 541, 584, 675
FaqI GGGAC 2 cut(s) 34, 579
FatI CATG 3 cut(s) 128, 169, 539
FauI CCCGC 1 cut(s) 590
FauNDI CATATG 1 cut(s) 85
Fnu4HI GCNGC 1 cut(s) 417
FriOI GRGCYC 1 cut(s) 606
Fsp4HI GCNGC 1 cut(s) 417
FspBI CTAG 2 cut(s) 17, 492
GluI GCNGC 1 cut(s) 417
GsaI CCCAGC 1 cut(s) 630
Hin1II CATG 3 cut(s) 132, 173, 543
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HinfI GANTC 3 cut(s) 242, 452, 614
HphI GGTGA 3 cut(s) 379, 671, 689
Hpy166II GTNNAC 1 cut(s) 378
Hpy188I TCNGA 4 cut(s) 157, 229, 252, 619
Hpy188III TCNNGA 3 cut(s) 144, 507, 640
Hpy8I GTNNAC 1 cut(s) 378
HpyAV CCTTC 1 cut(s) 553
HpyCH4III ACNGT 4 cut(s) 81, 120, 150, 322
HpyCH4V TGCA 2 cut(s) 326, 393
HpyF10VI GCNNNNNNNGC 3 cut(s) 425, 532, 689
Hsp92II CATG 3 cut(s) 132, 173, 543
Kzo9I GATC 2 cut(s) 503, 661
LmnI GCTCC 3 cut(s) 535, 601, 609
Lsp1109I GCAGC 1 cut(s) 403
LweI GCATC 1 cut(s) 670
MaeI CTAG 2 cut(s) 17, 492
MaeIII GTNAC 1 cut(s) 189
MalI GATC 2 cut(s) 505, 663
MboI GATC 2 cut(s) 503, 661
MboII GAAGA 3 cut(s) 230, 242, 665
MfeI CAATTG 1 cut(s) 609
MflI RGATCY 1 cut(s) 503
MhlI GDGCHC 4 cut(s) 64, 177, 532, 606
MluCI AATT 6 cut(s) 36, 271, 335, 342, 387, 609
MlyI GAGTC 1 cut(s) 623
MmeI TCCRAC 2 cut(s) 275, 649
MnlI CCTC 8 cut(s) 214, 274, 322, 363, 411, 556, 625, 712
MseI TTAA 3 cut(s) 338, 359, 717
MspR9I CCNGG 1 cut(s) 711
MunI CAATTG 1 cut(s) 609
Mva1269I GAATGC 2 cut(s) 34, 180
MvaI CCWGG 1 cut(s) 711
MwoI GCNNNNNNNGC 3 cut(s) 425, 532, 689
NdeI CATATG 1 cut(s) 85
NdeII GATC 2 cut(s) 503, 661
NlaIII CATG 3 cut(s) 132, 173, 543
NspV TTCGAA 1 cut(s) 455
PctI GAATGC 2 cut(s) 34, 180
PfeI GAWTC 2 cut(s) 242, 452
PkrI GCNGC 1 cut(s) 418
PleI GAGTC 1 cut(s) 622
PpsI GAGTC 1 cut(s) 622
Psp124BI GAGCTC 1 cut(s) 606
Psp6I CCWGG 1 cut(s) 709
PspFI CCCAGC 1 cut(s) 626
PspGI CCWGG 1 cut(s) 709
PspPI GGNCC 1 cut(s) 248
PsuI RGATCY 1 cut(s) 503
RsaI GTAC 2 cut(s) 125, 285
RsaNI GTAC 2 cut(s) 124, 284
SacI GAGCTC 1 cut(s) 606
SaqAI TTAA 3 cut(s) 338, 359, 717
SatI GCNGC 1 cut(s) 417
Sau3AI GATC 2 cut(s) 503, 661
Sau96I GGNCC 1 cut(s) 248
SchI GAGTC 1 cut(s) 623
ScrFI CCNGG 1 cut(s) 711
SduI GDGCHC 4 cut(s) 64, 177, 532, 606
SfaNI GCATC 1 cut(s) 670
SfcI CTRYAG 2 cut(s) 318, 480
SfuI TTCGAA 1 cut(s) 455
SinI GGWCC 1 cut(s) 248
Sse9I AATT 6 cut(s) 36, 271, 335, 342, 387, 609
SsiI CCGC 3 cut(s) 533, 597, 692
SspMI CTAG 2 cut(s) 17, 492
SstI GAGCTC 1 cut(s) 606
StyD4I CCNGG 1 cut(s) 709
StyI CCWWGG 1 cut(s) 16
TaaI ACNGT 4 cut(s) 81, 120, 150, 322
TaqI TCGA 3 cut(s) 137, 455, 508
TasI AATT 6 cut(s) 36, 271, 335, 342, 387, 609
TatI WGTACW 1 cut(s) 123
TfiI GAWTC 2 cut(s) 242, 452
Tru1I TTAA 3 cut(s) 338, 359, 717
Tru9I TTAA 3 cut(s) 338, 359, 717
TseI GCWGC 1 cut(s) 416
VpaK11BI GGWCC 1 cut(s) 248
XapI RAATTY 2 cut(s) 271, 387
XmaJI CCTAGG 1 cut(s) 16
XspI CTAG 2 cut(s) 17, 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.