MD09G1229700.v1.1

Serine incorporator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005052
Physical Location & Seq
Forward (+)
28373753 .. 28378501
4749 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1229700.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGTGGGCAGCTTCGTGCCTGGCATCATGCTGCGCTGCTTGCGCTTGCGATGCTTGCAGGACGGTGGTGTCAAGTATTAGCCGTCGGTCTGCTAGGATTGCATACTGGATTTCTCAGTTTAATGAGACACATAACAGGGAGTGGTTCGAAACAGATGCAGTGCTTCGTGTTAGCTTGGGGAATTTTCTCTTCTTCACTATTTTATCAGTTATGATGGTTGGTGTGAAGAATCAGAAGGATCCCCGTGATAGTTTGCATCATGGTGGATGGATGATGAAAGTTATATGCTGGTGTCTTTTGGTGATCTTCATGTTTTTCGTACCAAATGAGATTGTTAGCTTCTATGAGACAATTTCCAAGTTTGGCTCAGGATTTTTTCTTCTTGTACAAGTTGTACTTCTGTTGGATTTTGTTCATGGATGGAATGACAAATGGGTTGGATATGATGAGAAGTTCTGGTACATTGCACTTTTTGTTGTTTCTCTTGTTTGTTATCTGGCAACATTCGTCTTCTCTGGACTTCTTTTCCATTGGTTCACACCATCTGGACAAGACTGCGGGCTCAACACCTTCTTTATTGTCATGACTCTCATGTGTGTCTTCGGGTTTCTTATAGTGGCACTGCATCCTTCGGTAAGTGGCAGCATTTTGCCCGCGTCAGTTATATCTATGTACTGCACGTACCTCTGCTACAGTGCACTTGCCAGTGAACCTAGGGACTATGAGTGCAATGGTCTTCACAAGCACTCCAAAGCTGTTTCCACCGGGACCCTTACCTTTGGGTTGCTAACCACTGTTCTTTCTGTGGTTTATTCTGCTGTTCGTGCTGGATCTTCCACAACTCTTCTCTCCCCACCAAGTTCTCCTCGCTCAGGTGCTGGAAAGCCTCTGCTTCCATTAGACAAGGTTGATGAACACGAAGAAAAAGAGAAGTCCAAGCCAGTGTCATATTCATATTCGTTTTTCCACATCATCTTCTCTCTTGCTAGTATGTACTCGGCCATGCTTCTGACAGGGTGGTCAGCCTCAGTTGGGGAGAGCGGGAAGTTGGTGGATGTTGGGTGGCCATCTGTGTGGGTGAGGATGGTGACTAGTTGGGCAACTGCAGGTCTGTACATCTGGTCTCTTTTGGCTCCTATTCTGTTCCCCGAGAGGGAATTCTGA

Protein Analysis

388

Amino Acids

43.24

Weight (kDa)

6.78

Isoelectric Point (pI)

36.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serinc PF03348 43 - 385 4.9e-109 Serine incorporator (Serinc)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 67, 1018
Acc36I ACCTGC 1 cut(s) 1097
AccBSI CCGCTC 1 cut(s) 1041
AccII CGCG 1 cut(s) 656
AciI CCGC 3 cut(s) 558, 654, 1041
AclWI GGATC 3 cut(s) 233, 246, 838
AcoI YGGCCR 2 cut(s) 999, 1064
AcsI RAATTY 2 cut(s) 181, 1157
AfaI GTAC 8 cut(s) 321, 387, 396, 461, 674, 683, 995, 1115
AfiI CCNNNNNNNGG 3 cut(s) 872, 1032, 1153
AhlI ACTAGT 1 cut(s) 1091
AjnI CCWGG 1 cut(s) 18
AluBI AGCT 4 cut(s) 11, 174, 339, 755
AluI AGCT 4 cut(s) 11, 174, 339, 755
Alw21I GWGCWC 1 cut(s) 700
Alw26I GTCTC 3 cut(s) 119, 341, 1128
Alw44I GTGCAC 1 cut(s) 696
AlwI GGATC 3 cut(s) 233, 246, 838
AlwNI CAGNNNCTG 1 cut(s) 878
Ama87I CYCGRG 1 cut(s) 1148
AoxI GGCC 2 cut(s) 999, 1064
ApaLI GTGCAC 1 cut(s) 696
ApeKI GCWGC 4 cut(s) 8, 30, 35, 642
ApoI RAATTY 2 cut(s) 181, 1157
AspA2I CCTAGG 1 cut(s) 713
AspLEI GCGC 2 cut(s) 35, 44
AspS9I GGNCC 1 cut(s) 768
AsuC2I CCSGG 1 cut(s) 766
AsuHPI GGTGA 3 cut(s) 313, 1090, 1099
AsuII TTCGAA 1 cut(s) 147
AvaI CYCGRG 1 cut(s) 1148
AvaII GGWCC 1 cut(s) 768
AvrII CCTAGG 1 cut(s) 713
BaeGI GKGCMC 1 cut(s) 700
BalI TGGCCA 1 cut(s) 1066
BamHI GGATCC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 564
BarI GAAGNNNNNNTAC 2 cut(s) 443, 475
BbsI GAAGAC 3 cut(s) 502, 592, 728
Bbv12I GWGCWC 1 cut(s) 700
BbvI GCAGC 4 cut(s) 17, 20, 22, 654
BccI CCATC 6 cut(s) 208, 261, 414, 550, 1075, 1078
BceAI ACGGC 1 cut(s) 66
BcgI CGANNNNNNTGC 2 cut(s) 137, 171
BciT130I CCWGG 1 cut(s) 20
BcnI CCSGG 1 cut(s) 766
BcoDI GTCTC 3 cut(s) 119, 341, 1128
BcuI ACTAGT 1 cut(s) 1091
BfaI CTAG 4 cut(s) 93, 714, 987, 1092
BfmI CTRYAG 2 cut(s) 691, 1104
BfuAI ACCTGC 1 cut(s) 1097
BisI GCNGC 4 cut(s) 9, 31, 36, 643
BlnI CCTAGG 1 cut(s) 713
BlsI GCNGC 4 cut(s) 10, 32, 37, 644
Bme1390I CCNGG 2 cut(s) 20, 766
Bme18I GGWCC 1 cut(s) 768
BmeT110I CYCGRG 1 cut(s) 1148
BmgT120I GGNCC 1 cut(s) 768
BmiI GGNNCC 4 cut(s) 240, 769, 770, 1134
BmrFI CCNGG 2 cut(s) 20, 766
BmsI GCATC 5 cut(s) 32, 40, 145, 265, 634
BpiI GAAGAC 3 cut(s) 502, 592, 728
Bpu10I CCTNAGC 2 cut(s) 367, 871
Bpu14I TTCGAA 1 cut(s) 147
BpuMI CCSGG 1 cut(s) 766
BsaAI YACGTR 1 cut(s) 681
BsaI GGTCTC 1 cut(s) 1128
BsaJI CCNNGG 1 cut(s) 713
BsaXI ACNNNNNCTCC 2 cut(s) 731, 761
Bsc4I CCNNNNNNNGG 3 cut(s) 872, 1032, 1153
Bse1I ACTGG 3 cut(s) 110, 705, 941
Bse3DI GCAATG 2 cut(s) 462, 736
BseBI CCWGG 1 cut(s) 20
BseDI CCNNGG 1 cut(s) 713
BseGI GGATG 6 cut(s) 272, 276, 425, 625, 1060, 1089
BseLI CCNNNNNNNGG 3 cut(s) 872, 1032, 1153
BseMI GCAATG 2 cut(s) 462, 736
BseMII CTCAG 4 cut(s) 128, 381, 885, 1041
BseNI ACTGG 3 cut(s) 110, 705, 941
BseRI GAGGAG 1 cut(s) 855
BseSI GKGCMC 1 cut(s) 700
BseXI GCAGC 4 cut(s) 17, 20, 22, 654
BsgI GTGCAG 1 cut(s) 661
Bsh1236I CGCG 1 cut(s) 656
BshFI GGCC 2 cut(s) 1001, 1066
BsiHKAI GWGCWC 1 cut(s) 700
BsiHKCI CYCGRG 1 cut(s) 1148
BsiSI CCGG 1 cut(s) 765
BslFI GGGAC 2 cut(s) 731, 781
BslI CCNNNNNNNGG 3 cut(s) 872, 1032, 1153
BsmAI GTCTC 3 cut(s) 119, 341, 1128
BsmFI GGGAC 2 cut(s) 731, 781
BsnI GGCC 2 cut(s) 1001, 1066
Bso31I GGTCTC 1 cut(s) 1128
BsoBI CYCGRG 1 cut(s) 1148
Bsp119I TTCGAA 1 cut(s) 147
Bsp1286I GDGCHC 2 cut(s) 564, 700
Bsp1407I TGTACA 2 cut(s) 385, 1113
Bsp143I GATC 3 cut(s) 238, 303, 830
BspACI CCGC 3 cut(s) 558, 654, 1041
BspANI GGCC 2 cut(s) 1001, 1066
BspCNI CTCAG 4 cut(s) 127, 380, 884, 1040
BspFNI CGCG 1 cut(s) 656
BspHI TCATGA 1 cut(s) 582
BspLI GGNNCC 4 cut(s) 240, 769, 770, 1134
BspMAI CTGCAG 1 cut(s) 1108
BspMI ACCTGC 1 cut(s) 1097
BspPI GGATC 3 cut(s) 233, 246, 838
BspT104I TTCGAA 1 cut(s) 147
BspTNI GGTCTC 1 cut(s) 1128
BsrBI CCGCTC 1 cut(s) 1041
BsrDI GCAATG 2 cut(s) 462, 736
BsrGI TGTACA 2 cut(s) 385, 1113
BsrI ACTGG 3 cut(s) 110, 705, 941
BssECI CCNNGG 1 cut(s) 713
BssMI GATC 3 cut(s) 238, 303, 830
BssT1I CCWWGG 1 cut(s) 713
Bst2UI CCWGG 1 cut(s) 20
Bst4CI ACNGT 3 cut(s) 64, 695, 796
Bst6I CTCTTC 2 cut(s) 194, 849
BstAUI TGTACA 2 cut(s) 385, 1113
BstBAI YACGTR 1 cut(s) 681
BstBI TTCGAA 1 cut(s) 147
BstC8I GCNNGC 5 cut(s) 40, 46, 55, 560, 654
BstDEI CTNAG 4 cut(s) 114, 367, 871, 1027
BstENI CCTNNNNNAGG 1 cut(s) 870
BstF5I GGATG 6 cut(s) 272, 276, 425, 625, 1060, 1089
BstFNI CGCG 1 cut(s) 656
BstHHI GCGC 2 cut(s) 35, 44
BstKTI GATC 3 cut(s) 241, 306, 833
BstMAI GTCTC 3 cut(s) 119, 341, 1128
BstMBI GATC 3 cut(s) 238, 303, 830
BstMWI GCNNNNNNNGC 6 cut(s) 39, 41, 50, 54, 98, 824
BstNI CCWGG 1 cut(s) 20
BstSCI CCNGG 2 cut(s) 18, 764
BstSFI CTRYAG 2 cut(s) 691, 1104
BstSLI GKGCMC 1 cut(s) 700
BstUI CGCG 1 cut(s) 656
BstV1I GCAGC 4 cut(s) 17, 20, 22, 654
BstV2I GAAGAC 3 cut(s) 502, 592, 728
BstX2I RGATCY 2 cut(s) 238, 830
BstXI CCANNNNNNTGG 1 cut(s) 1074
BstYI RGATCY 2 cut(s) 238, 830
BsuRI GGCC 2 cut(s) 1001, 1066
BtgZI GCGATG 1 cut(s) 63
BtsCI GGATG 6 cut(s) 272, 276, 425, 625, 1060, 1089
BtsI GCAGTG 2 cut(s) 165, 620
BtsIMutI CAGTG 6 cut(s) 165, 620, 700, 712, 792, 948
BveI ACCTGC 1 cut(s) 1097
Cac8I GCNNGC 5 cut(s) 40, 46, 55, 560, 654
CaiI CAGNNNCTG 1 cut(s) 878
CciI TCATGA 1 cut(s) 582
CfoI GCGC 2 cut(s) 35, 44
Cfr13I GGNCC 1 cut(s) 768
CseI GACGC 1 cut(s) 645
Csp6I GTAC 8 cut(s) 320, 386, 395, 460, 673, 682, 994, 1114
CviAII CATG 7 cut(s) 27, 260, 310, 416, 583, 592, 1003
CviQI GTAC 8 cut(s) 320, 386, 395, 460, 673, 682, 994, 1114
DdeI CTNAG 4 cut(s) 114, 367, 871, 1027
DpnI GATC 3 cut(s) 240, 305, 832
DpnII GATC 3 cut(s) 238, 303, 830
DrdI GACNNNNNNGTC 2 cut(s) 67, 1018
DseDI GACNNNNNNGTC 2 cut(s) 67, 1018
EaeI YGGCCR 2 cut(s) 999, 1064
Eam1104I CTCTTC 2 cut(s) 194, 849
EarI CTCTTC 2 cut(s) 194, 849
Eco130I CCWWGG 1 cut(s) 713
Eco24I GRGCYC 1 cut(s) 564
Eco31I GGTCTC 1 cut(s) 1128
Eco47I GGWCC 1 cut(s) 768
Eco88I CYCGRG 1 cut(s) 1148
EcoNI CCTNNNNNAGG 1 cut(s) 870
EcoO109I RGGNCCY 1 cut(s) 768
EcoRI GAATTC 1 cut(s) 1157
EcoRII CCWGG 1 cut(s) 18
EcoT14I CCWWGG 1 cut(s) 713
EcoT38I GRGCYC 1 cut(s) 564
ErhI CCWWGG 1 cut(s) 713
FaeI CATG 7 cut(s) 30, 263, 313, 419, 586, 595, 1006
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FaqI GGGAC 2 cut(s) 731, 781
FatI CATG 7 cut(s) 26, 259, 309, 415, 582, 591, 1002
FauI CCCGC 3 cut(s) 551, 661, 1034
Fnu4HI GCNGC 4 cut(s) 9, 31, 36, 643
FokI GGATG 6 cut(s) 279, 283, 432, 612, 1067, 1096
FriOI GRGCYC 1 cut(s) 564
Fsp4HI GCNGC 4 cut(s) 9, 31, 36, 643
FspBI CTAG 4 cut(s) 93, 714, 987, 1092
GlaI GCGC 2 cut(s) 34, 43
GluI GCNGC 4 cut(s) 9, 31, 36, 643
HaeIII GGCC 2 cut(s) 1001, 1066
HapII CCGG 1 cut(s) 765
HgaI GACGC 1 cut(s) 645
HhaI GCGC 2 cut(s) 35, 44
Hin1II CATG 7 cut(s) 30, 263, 313, 419, 586, 595, 1006
Hin6I GCGC 2 cut(s) 33, 42
HinP1I GCGC 2 cut(s) 33, 42
HinfI GANTC 2 cut(s) 229, 586
HpaII CCGG 1 cut(s) 765
HphI GGTGA 3 cut(s) 313, 1090, 1099
Hpy166II GTNNAC 3 cut(s) 537, 698, 710
Hpy188I TCNGA 3 cut(s) 234, 1011, 1163
Hpy188III TCNNGA 4 cut(s) 369, 516, 546, 583
Hpy8I GTNNAC 3 cut(s) 537, 698, 710
Hpy99I CGWCG 1 cut(s) 87
HpyAV CCTTC 3 cut(s) 229, 580, 639
HpyCH4III ACNGT 3 cut(s) 64, 695, 796
HpyCH4IV ACGT 1 cut(s) 680
HpyF10VI GCNNNNNNNGC 6 cut(s) 39, 41, 50, 54, 98, 824
HpyF3I CTNAG 4 cut(s) 114, 367, 871, 1027
HpySE526I ACGT 1 cut(s) 680
Hsp92II CATG 7 cut(s) 30, 263, 313, 419, 586, 595, 1006
HspAI GCGC 2 cut(s) 33, 42
KflI GGGWCCC 1 cut(s) 768
Kzo9I GATC 3 cut(s) 238, 303, 830
LmnI GCTCC 1 cut(s) 1138
Lsp1109I GCAGC 4 cut(s) 17, 20, 22, 654
LweI GCATC 5 cut(s) 32, 40, 145, 265, 634
MaeI CTAG 4 cut(s) 93, 714, 987, 1092
MaeII ACGT 1 cut(s) 680
MaeIII GTNAC 1 cut(s) 1087
MalI GATC 3 cut(s) 240, 305, 832
MbiI CCGCTC 1 cut(s) 1041
MboI GATC 3 cut(s) 238, 303, 830
MflI RGATCY 2 cut(s) 238, 830
MhlI GDGCHC 2 cut(s) 564, 700
MlsI TGGCCA 1 cut(s) 1066
MluCI AATT 3 cut(s) 181, 351, 1157
MluNI TGGCCA 1 cut(s) 1066
MlyI GAGTC 1 cut(s) 580
MmeI TCCRAC 2 cut(s) 384, 418
MnlI CCTC 6 cut(s) 695, 876, 897, 1036, 1074, 1146
Mox20I TGGCCA 1 cut(s) 1066
MscI TGGCCA 1 cut(s) 1066
MseI TTAA 1 cut(s) 120
MslI CAYNNNNRTG 2 cut(s) 261, 1072
Msp20I TGGCCA 1 cut(s) 1066
MspI CCGG 1 cut(s) 765
MspR9I CCNGG 2 cut(s) 20, 766
MvaI CCWGG 1 cut(s) 20
MvnI CGCG 1 cut(s) 656
MwoI GCNNNNNNNGC 6 cut(s) 39, 41, 50, 54, 98, 824
NciI CCSGG 1 cut(s) 766
NdeII GATC 3 cut(s) 238, 303, 830
NlaIII CATG 7 cut(s) 30, 263, 313, 419, 586, 595, 1006
NlaIV GGNNCC 4 cut(s) 240, 769, 770, 1134
NmeAIII GCCGAG 1 cut(s) 977
NmuCI GTSAC 1 cut(s) 1087
NspV TTCGAA 1 cut(s) 147
PagI TCATGA 1 cut(s) 582
PfeI GAWTC 1 cut(s) 229
PkrI GCNGC 4 cut(s) 10, 32, 37, 644
PleI GAGTC 1 cut(s) 580
PpsI GAGTC 1 cut(s) 580
Ppu21I YACGTR 1 cut(s) 681
PpuMI RGGWCCY 1 cut(s) 768
Psp5II RGGWCCY 1 cut(s) 768
Psp6I CCWGG 1 cut(s) 18
PspGI CCWGG 1 cut(s) 18
PspN4I GGNNCC 4 cut(s) 240, 769, 770, 1134
PspPI GGNCC 1 cut(s) 768
PspPPI RGGWCCY 1 cut(s) 768
PstI CTGCAG 1 cut(s) 1108
PstNI CAGNNNCTG 1 cut(s) 878
PsuI RGATCY 2 cut(s) 238, 830
RsaI GTAC 8 cut(s) 321, 387, 396, 461, 674, 683, 995, 1115
RsaNI GTAC 8 cut(s) 320, 386, 395, 460, 673, 682, 994, 1114
RseI CAYNNNNRTG 2 cut(s) 261, 1072
SaqAI TTAA 1 cut(s) 120
SatI GCNGC 4 cut(s) 9, 31, 36, 643
Sau3AI GATC 3 cut(s) 238, 303, 830
Sau96I GGNCC 1 cut(s) 768
SchI GAGTC 1 cut(s) 580
ScrFI CCNGG 2 cut(s) 20, 766
SduI GDGCHC 2 cut(s) 564, 700
SfaNI GCATC 5 cut(s) 32, 40, 145, 265, 634
SfcI CTRYAG 2 cut(s) 691, 1104
SfuI TTCGAA 1 cut(s) 147
SinI GGWCC 1 cut(s) 768
SmiMI CAYNNNNRTG 2 cut(s) 261, 1072
SpeI ACTAGT 1 cut(s) 1091
Sse9I AATT 3 cut(s) 181, 351, 1157
SsiI CCGC 3 cut(s) 558, 654, 1041
SspMI CTAG 4 cut(s) 93, 714, 987, 1092
StyD4I CCNGG 2 cut(s) 18, 764
StyI CCWWGG 1 cut(s) 713
TaaI ACNGT 3 cut(s) 64, 695, 796
TaiI ACGT 1 cut(s) 683
TaqI TCGA 1 cut(s) 147
TaqII GACCGA 1 cut(s) 75
TasI AATT 3 cut(s) 181, 351, 1157
TatI WGTACW 5 cut(s) 385, 394, 672, 993, 1113
TfiI GAWTC 1 cut(s) 229
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
TscAI CASTG 6 cut(s) 165, 627, 700, 712, 799, 948
TseFI GTSAC 1 cut(s) 1087
TseI GCWGC 4 cut(s) 8, 30, 35, 642
Tsp45I GTSAC 1 cut(s) 1087
TspDTI ATGAA 5 cut(s) 290, 298, 404, 927, 942
TspRI CASTG 6 cut(s) 165, 627, 700, 712, 799, 948
VneI GTGCAC 1 cut(s) 696
VpaK11BI GGWCC 1 cut(s) 768
XagI CCTNNNNNAGG 1 cut(s) 870
XapI RAATTY 2 cut(s) 181, 1157
XmaJI CCTAGG 1 cut(s) 713
XspI CTAG 4 cut(s) 93, 714, 987, 1092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.