pycom17g23100

Serine incorporator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005052
Physical Location & Seq
Forward (+)
21507945 .. 21510832
2888 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g23100.1

Sequence Viewer

Length: 1254 bp
ATGTGGGCAGCTTCGTGCCTGGCATCATGCTGCGCTGCTTGCGCTTGCGATGCTTGCCGGACAGTGGTGTCAAGTATTAGCCGCCGGTCTGCTAGGATTGCTTACTGTGGGCTCTTTGCGCTTTCTTTGATAGTTTCATGGATTCTCCGTGAGGTTGCGGCTCCTCTCCTCGAAAAGATTCCTTGGATTAATCAGTTTAATGAGACACATAACAGGGAGTGGTTCGAAACAGATGCAGTGCTTCGTGTTAGCTTGGGAAATTTTCTCTTCTTCACTATTTTATCAGTTATGATGGTTGGTGTGAAGAATCAGAAGGATCGATCTGGTTTGACCCGTGATAGTTTGCATCATGGTGGATGGATGATGAAAGTTATATGCTGGTGTCTCTTAGTGATCTTCATGTTTTTTGTACCAAATGAGATTGTTAGCTTCTATGAGACAATTTCAAAGTTTGGCTCGGGATTTTTTCTTCTTGTACAAGTTGTACTTCTGTTGGATTTTGTTCATGGATGGAATGACAAATGGGTTGGATATGATGAGAAGTTCTGGTACGTTGCACTTTTTGTTGTTTCTCTTGTTTGTTATCTGGCAACATTTGTCTTCTCTGGACTTCTTTTCCATTGGTTCACACCATCTGGACAAGACTGTGGGCTCAACACCTTCTTTATTGTCATGACTCTCATGTGTGTCTTCGTGTTTCTTATAGTGGCACTGCATCCTGCTGTAAATGGCAGCATTTTGCCAGCATCAGTTATATCTATGTACTGCACATACCTCTGCTACAGTGCACTTGCCAGTGAACCTAGGGACTATGAGTGCAATGGTCTTCACAAGCACTCCAAAGCTGTTTCCACTGGGACACTTACCTTCGGTTTGCTAACCACTGTTCTTTCTGTGGTGTATTCTGCTGTTCGTGCTGGATCCTCCACAACACTTCTCTCCCCACCAGGTTCTCCCCGCGCAGGCGCTGGAAAGCCTCTGCTTCCATTAGACAAGGTTGATGAACACGAAGAAAAAGAGAAGTGCAAGCCAGTATCATATTCGTATTCGTTTTTCCACATAATCTTCTCTCTTGCTAGCATGTACTCGGCCATGCTTCTGACGGGGTGGTCAACCTCTGTTGGGGAGAGTGGGAAGTTGGTGGACGTTGGGTGGCCATCTGTGTGGGTGAGGATGGTGACTGGTTGGGCAACTGCAGGTCTGTACATCTGGTCTCTTTTGGCTCCTATTCTGTTCCCCGAGAGGGAATTCTGA

Protein Analysis

418

Amino Acids

46.53

Weight (kDa)

7.09

Isoelectric Point (pI)

33.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serinc PF03348 8 - 415 9e-118 Serine incorporator (Serinc)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 67, 1108
Acc36I ACCTGC 1 cut(s) 1187
AccII CGCG 1 cut(s) 960
AciI CCGC 3 cut(s) 82, 158, 958
AclWI GGATC 3 cut(s) 324, 915, 928
AcoI YGGCCR 2 cut(s) 1089, 1154
AcsI RAATTY 2 cut(s) 259, 1247
AfaI GTAC 7 cut(s) 411, 477, 486, 551, 764, 1085, 1205
AfiI CCNNNNNNNGG 4 cut(s) 64, 962, 1122, 1243
AgsI TTSAA 1 cut(s) 447
AjnI CCWGG 2 cut(s) 18, 946
AluBI AGCT 4 cut(s) 11, 252, 429, 845
AluI AGCT 4 cut(s) 11, 252, 429, 845
Alw21I GWGCWC 1 cut(s) 790
Alw26I GTCTC 4 cut(s) 197, 389, 431, 1218
Alw44I GTGCAC 1 cut(s) 786
AlwI GGATC 3 cut(s) 324, 915, 928
AlwNI CAGNNNCTG 1 cut(s) 968
Ama87I CYCGRG 2 cut(s) 457, 1238
AoxI GGCC 2 cut(s) 1089, 1154
ApaLI GTGCAC 1 cut(s) 786
ApeKI GCWGC 4 cut(s) 8, 30, 35, 732
ApoI RAATTY 2 cut(s) 259, 1247
AseI ATTAAT 1 cut(s) 189
Asp700I GAANNNNTTC 1 cut(s) 177
AspA2I CCTAGG 1 cut(s) 803
AspLEI GCGC 5 cut(s) 35, 44, 121, 962, 968
AsuHPI GGTGA 2 cut(s) 1180, 1189
AsuII TTCGAA 1 cut(s) 225
AsuNHI GCTAGC 1 cut(s) 1076
AvaI CYCGRG 2 cut(s) 457, 1238
AvrII CCTAGG 1 cut(s) 803
BaeGI GKGCMC 1 cut(s) 790
BalI TGGCCA 1 cut(s) 1156
BamHI GGATCC 1 cut(s) 920
BanII GRGCYC 2 cut(s) 114, 654
BarI GAAGNNNNNNTAC 2 cut(s) 533, 565
BbsI GAAGAC 3 cut(s) 592, 682, 818
Bbv12I GWGCWC 1 cut(s) 790
BbvI GCAGC 4 cut(s) 17, 20, 22, 744
BccI CCATC 6 cut(s) 286, 351, 504, 640, 1165, 1168
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 2 cut(s) 20, 948
BcoDI GTCTC 4 cut(s) 197, 389, 431, 1218
BfaI CTAG 3 cut(s) 93, 804, 1077
BfmI CTRYAG 2 cut(s) 781, 1194
BfoI RGCGCY 1 cut(s) 969
BfuAI ACCTGC 1 cut(s) 1187
BisI GCNGC 6 cut(s) 9, 31, 36, 82, 159, 733
BlnI CCTAGG 1 cut(s) 803
BlsI GCNGC 6 cut(s) 10, 32, 37, 83, 160, 734
Bme1390I CCNGG 2 cut(s) 20, 948
BmeT110I CYCGRG 2 cut(s) 457, 1238
BmiI GGNNCC 3 cut(s) 162, 922, 1224
BmrFI CCNGG 2 cut(s) 20, 948
BmrI ACTGGG 1 cut(s) 864
BmsI GCATC 6 cut(s) 32, 40, 223, 355, 724, 755
BmtI GCTAGC 1 cut(s) 1080
BmuI ACTGGG 1 cut(s) 864
BpiI GAAGAC 3 cut(s) 592, 682, 818
Bpu14I TTCGAA 1 cut(s) 225
Bsa29I ATCGAT 1 cut(s) 319
BsaI GGTCTC 1 cut(s) 1218
BsaJI CCNNGG 2 cut(s) 182, 803
BsaXI ACNNNNNCTCC 2 cut(s) 821, 851
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 962, 1122, 1243
Bse118I RCCGGY 1 cut(s) 84
Bse1I ACTGG 4 cut(s) 795, 859, 1031, 1186
Bse3DI GCAATG 1 cut(s) 826
BseBI CCWGG 2 cut(s) 20, 948
BseCI ATCGAT 1 cut(s) 319
BseDI CCNNGG 2 cut(s) 182, 803
BseGI GGATG 5 cut(s) 362, 366, 515, 715, 1179
BseLI CCNNNNNNNGG 4 cut(s) 64, 962, 1122, 1243
BseMI GCAATG 1 cut(s) 826
BseNI ACTGG 4 cut(s) 795, 859, 1031, 1186
BseRI GAGGAG 2 cut(s) 153, 158
BseSI GKGCMC 1 cut(s) 790
BseXI GCAGC 4 cut(s) 17, 20, 22, 744
BsgI GTGCAG 1 cut(s) 751
Bsh1236I CGCG 1 cut(s) 960
BshFI GGCC 2 cut(s) 1091, 1156
BshVI ATCGAT 1 cut(s) 319
BsiHKAI GWGCWC 1 cut(s) 790
BsiHKCI CYCGRG 2 cut(s) 457, 1238
BsiSI CCGG 2 cut(s) 58, 85
BslFI GGGAC 2 cut(s) 821, 871
BslI CCNNNNNNNGG 4 cut(s) 64, 962, 1122, 1243
BsmAI GTCTC 4 cut(s) 197, 389, 431, 1218
BsmFI GGGAC 2 cut(s) 821, 871
BsnI GGCC 2 cut(s) 1091, 1156
Bso31I GGTCTC 1 cut(s) 1218
BsoBI CYCGRG 2 cut(s) 457, 1238
Bsp119I TTCGAA 1 cut(s) 225
Bsp1286I GDGCHC 3 cut(s) 114, 654, 790
Bsp1407I TGTACA 2 cut(s) 475, 1203
Bsp143I GATC 4 cut(s) 316, 320, 393, 920
BspACI CCGC 3 cut(s) 82, 158, 958
BspANI GGCC 2 cut(s) 1091, 1156
BspDI ATCGAT 1 cut(s) 319
BspFNI CGCG 1 cut(s) 960
BspHI TCATGA 1 cut(s) 672
BspLI GGNNCC 3 cut(s) 162, 922, 1224
BspMAI CTGCAG 1 cut(s) 1198
BspMI ACCTGC 1 cut(s) 1187
BspOI GCTAGC 1 cut(s) 1080
BspPI GGATC 3 cut(s) 324, 915, 928
BspT104I TTCGAA 1 cut(s) 225
BspTNI GGTCTC 1 cut(s) 1218
BsrDI GCAATG 1 cut(s) 826
BsrFI RCCGGY 1 cut(s) 84
BsrGI TGTACA 2 cut(s) 475, 1203
BsrI ACTGG 4 cut(s) 795, 859, 1031, 1186
BssAI RCCGGY 1 cut(s) 84
BssECI CCNNGG 2 cut(s) 182, 803
BssMI GATC 4 cut(s) 316, 320, 393, 920
BssT1I CCWWGG 2 cut(s) 182, 803
Bst2UI CCWGG 2 cut(s) 20, 948
Bst4CI ACNGT 5 cut(s) 64, 107, 647, 785, 886
Bst6I CTCTTC 1 cut(s) 272
BstAUI TGTACA 2 cut(s) 475, 1203
BstBI TTCGAA 1 cut(s) 225
BstC8I GCNNGC 7 cut(s) 40, 46, 55, 744, 964, 1028, 1078
BstDEI CTNAG 1 cut(s) 388
BstF5I GGATG 5 cut(s) 362, 366, 515, 715, 1179
BstFNI CGCG 1 cut(s) 960
BstH2I RGCGCY 1 cut(s) 969
BstHHI GCGC 5 cut(s) 35, 44, 121, 962, 968
BstKTI GATC 4 cut(s) 319, 323, 396, 923
BstMAI GTCTC 4 cut(s) 197, 389, 431, 1218
BstMBI GATC 4 cut(s) 316, 320, 393, 920
BstMWI GCNNNNNNNGC 7 cut(s) 39, 41, 50, 54, 98, 118, 914
BstNI CCWGG 2 cut(s) 20, 948
BstNSI RCATGY 1 cut(s) 1084
BstSCI CCNGG 2 cut(s) 18, 946
BstSFI CTRYAG 2 cut(s) 781, 1194
BstSLI GKGCMC 1 cut(s) 790
BstUI CGCG 1 cut(s) 960
BstV1I GCAGC 4 cut(s) 17, 20, 22, 744
BstV2I GAAGAC 3 cut(s) 592, 682, 818
BstX2I RGATCY 1 cut(s) 920
BstXI CCANNNNNNTGG 1 cut(s) 1164
BstYI RGATCY 1 cut(s) 920
Bsu15I ATCGAT 1 cut(s) 319
BsuRI GGCC 2 cut(s) 1091, 1156
BsuTUI ATCGAT 1 cut(s) 319
BtgZI GCGATG 1 cut(s) 63
BtsCI GGATG 5 cut(s) 362, 366, 515, 715, 1179
BtsI GCAGTG 2 cut(s) 243, 710
BtsIMutI CAGTG 7 cut(s) 69, 243, 710, 790, 802, 852, 882
BveI ACCTGC 1 cut(s) 1187
Cac8I GCNNGC 7 cut(s) 40, 46, 55, 744, 964, 1028, 1078
CaiI CAGNNNCTG 1 cut(s) 968
CciI TCATGA 1 cut(s) 672
CfoI GCGC 5 cut(s) 35, 44, 121, 962, 968
Cfr10I RCCGGY 1 cut(s) 84
ClaI ATCGAT 1 cut(s) 319
CsiI ACCWGGT 1 cut(s) 946
Csp6I GTAC 7 cut(s) 410, 476, 485, 550, 763, 1084, 1204
CviAII CATG 9 cut(s) 27, 138, 350, 400, 506, 673, 682, 1081, 1093
CviQI GTAC 7 cut(s) 410, 476, 485, 550, 763, 1084, 1204
DdeI CTNAG 1 cut(s) 388
DpnI GATC 4 cut(s) 318, 322, 395, 922
DpnII GATC 4 cut(s) 316, 320, 393, 920
DrdI GACNNNNNNGTC 2 cut(s) 67, 1108
DseDI GACNNNNNNGTC 2 cut(s) 67, 1108
EaeI YGGCCR 2 cut(s) 1089, 1154
Eam1104I CTCTTC 1 cut(s) 272
EarI CTCTTC 1 cut(s) 272
Eco130I CCWWGG 2 cut(s) 182, 803
Eco24I GRGCYC 2 cut(s) 114, 654
Eco31I GGTCTC 1 cut(s) 1218
Eco88I CYCGRG 2 cut(s) 457, 1238
EcoRI GAATTC 1 cut(s) 1247
EcoRII CCWGG 2 cut(s) 18, 946
EcoT14I CCWWGG 2 cut(s) 182, 803
EcoT38I GRGCYC 2 cut(s) 114, 654
ErhI CCWWGG 2 cut(s) 182, 803
FaeI CATG 9 cut(s) 30, 141, 353, 403, 509, 676, 685, 1084, 1096
FalI AAGNNNNNCTT 2 cut(s) 471, 503
FaqI GGGAC 2 cut(s) 821, 871
FatI CATG 9 cut(s) 26, 137, 349, 399, 505, 672, 681, 1080, 1092
FauI CCCGC 1 cut(s) 965
Fnu4HI GCNGC 6 cut(s) 9, 31, 36, 82, 159, 733
FokI GGATG 5 cut(s) 369, 373, 522, 702, 1186
FriOI GRGCYC 2 cut(s) 114, 654
Fsp4HI GCNGC 6 cut(s) 9, 31, 36, 82, 159, 733
FspBI CTAG 3 cut(s) 93, 804, 1077
GlaI GCGC 5 cut(s) 34, 43, 120, 961, 967
GluI GCNGC 6 cut(s) 9, 31, 36, 82, 159, 733
HaeII RGCGCY 1 cut(s) 969
HaeIII GGCC 2 cut(s) 1091, 1156
HapII CCGG 2 cut(s) 58, 85
HhaI GCGC 5 cut(s) 35, 44, 121, 962, 968
Hin1II CATG 9 cut(s) 30, 141, 353, 403, 509, 676, 685, 1084, 1096
Hin6I GCGC 5 cut(s) 33, 42, 119, 960, 966
HinP1I GCGC 5 cut(s) 33, 42, 119, 960, 966
HincII GTYRAC 1 cut(s) 1113
HindII GTYRAC 1 cut(s) 1113
HinfI GANTC 4 cut(s) 142, 178, 307, 676
HpaII CCGG 2 cut(s) 58, 85
HphI GGTGA 2 cut(s) 1180, 1189
Hpy166II GTNNAC 5 cut(s) 627, 788, 800, 1113, 1144
Hpy188I TCNGA 3 cut(s) 312, 1101, 1253
Hpy188III TCNNGA 4 cut(s) 459, 606, 636, 673
Hpy8I GTNNAC 5 cut(s) 627, 788, 800, 1113, 1144
HpyAV CCTTC 3 cut(s) 307, 670, 877
HpyCH4III ACNGT 5 cut(s) 64, 107, 647, 785, 886
HpyCH4IV ACGT 2 cut(s) 552, 1146
HpyCH4V TGCA 9 cut(s) 236, 346, 557, 715, 768, 788, 819, 1026, 1196
HpyF10VI GCNNNNNNNGC 7 cut(s) 39, 41, 50, 54, 98, 118, 914
HpyF3I CTNAG 1 cut(s) 388
HpySE526I ACGT 2 cut(s) 552, 1146
Hsp92II CATG 9 cut(s) 30, 141, 353, 403, 509, 676, 685, 1084, 1096
HspAI GCGC 5 cut(s) 33, 42, 119, 960, 966
Kzo9I GATC 4 cut(s) 316, 320, 393, 920
LmnI GCTCC 2 cut(s) 166, 1228
Lsp1109I GCAGC 4 cut(s) 17, 20, 22, 744
LweI GCATC 6 cut(s) 32, 40, 223, 355, 724, 755
MabI ACCWGGT 1 cut(s) 946
MaeI CTAG 3 cut(s) 93, 804, 1077
MaeII ACGT 2 cut(s) 552, 1146
MaeIII GTNAC 1 cut(s) 1177
MalI GATC 4 cut(s) 318, 322, 395, 922
MboI GATC 4 cut(s) 316, 320, 393, 920
MflI RGATCY 1 cut(s) 920
MhlI GDGCHC 3 cut(s) 114, 654, 790
MlsI TGGCCA 1 cut(s) 1156
MluCI AATT 3 cut(s) 259, 441, 1247
MluNI TGGCCA 1 cut(s) 1156
MlyI GAGTC 1 cut(s) 670
MmeI TCCRAC 2 cut(s) 474, 508
MnlI CCTC 9 cut(s) 145, 174, 179, 785, 934, 987, 1126, 1164, 1236
Mox20I TGGCCA 1 cut(s) 1156
MroXI GAANNNNTTC 1 cut(s) 177
MscI TGGCCA 1 cut(s) 1156
MseI TTAA 2 cut(s) 189, 198
MslI CAYNNNNRTG 2 cut(s) 351, 1162
Msp20I TGGCCA 1 cut(s) 1156
MspI CCGG 2 cut(s) 58, 85
MspR9I CCNGG 2 cut(s) 20, 948
MvaI CCWGG 2 cut(s) 20, 948
MvnI CGCG 1 cut(s) 960
MwoI GCNNNNNNNGC 7 cut(s) 39, 41, 50, 54, 98, 118, 914
NdeII GATC 4 cut(s) 316, 320, 393, 920
NheI GCTAGC 1 cut(s) 1076
NlaIII CATG 9 cut(s) 30, 141, 353, 403, 509, 676, 685, 1084, 1096
NlaIV GGNNCC 3 cut(s) 162, 922, 1224
NmeAIII GCCGAG 1 cut(s) 1067
NmuCI GTSAC 1 cut(s) 1177
NspI RCATGY 1 cut(s) 1084
NspV TTCGAA 1 cut(s) 225
PagI TCATGA 1 cut(s) 672
PdmI GAANNNNTTC 1 cut(s) 177
PfeI GAWTC 3 cut(s) 142, 178, 307
PkrI GCNGC 6 cut(s) 10, 32, 37, 83, 160, 734
PleI GAGTC 1 cut(s) 670
PpsI GAGTC 1 cut(s) 670
PshBI ATTAAT 1 cut(s) 189
Psp6I CCWGG 2 cut(s) 18, 946
PspGI CCWGG 2 cut(s) 18, 946
PspN4I GGNNCC 3 cut(s) 162, 922, 1224
PstI CTGCAG 1 cut(s) 1198
PstNI CAGNNNCTG 1 cut(s) 968
PsuI RGATCY 1 cut(s) 920
RsaI GTAC 7 cut(s) 411, 477, 486, 551, 764, 1085, 1205
RsaNI GTAC 7 cut(s) 410, 476, 485, 550, 763, 1084, 1204
RseI CAYNNNNRTG 2 cut(s) 351, 1162
SaqAI TTAA 2 cut(s) 189, 198
SatI GCNGC 6 cut(s) 9, 31, 36, 82, 159, 733
Sau3AI GATC 4 cut(s) 316, 320, 393, 920
SchI GAGTC 1 cut(s) 670
ScrFI CCNGG 2 cut(s) 20, 948
SduI GDGCHC 3 cut(s) 114, 654, 790
SexAI ACCWGGT 1 cut(s) 946
SfaNI GCATC 6 cut(s) 32, 40, 223, 355, 724, 755
SfcI CTRYAG 2 cut(s) 781, 1194
SfuI TTCGAA 1 cut(s) 225
SmiMI CAYNNNNRTG 2 cut(s) 351, 1162
Sse9I AATT 3 cut(s) 259, 441, 1247
SsiI CCGC 3 cut(s) 82, 158, 958
SspMI CTAG 3 cut(s) 93, 804, 1077
StyD4I CCNGG 2 cut(s) 18, 946
StyI CCWWGG 2 cut(s) 182, 803
TaaI ACNGT 5 cut(s) 64, 107, 647, 785, 886
TaiI ACGT 2 cut(s) 555, 1149
TaqI TCGA 3 cut(s) 171, 225, 319
TasI AATT 3 cut(s) 259, 441, 1247
TatI WGTACW 5 cut(s) 475, 484, 762, 1083, 1203
TauI GCSGC 2 cut(s) 84, 161
TfiI GAWTC 3 cut(s) 142, 178, 307
Tru1I TTAA 2 cut(s) 189, 198
Tru9I TTAA 2 cut(s) 189, 198
TscAI CASTG 7 cut(s) 69, 243, 717, 790, 802, 859, 889
TseFI GTSAC 1 cut(s) 1177
TseI GCWGC 4 cut(s) 8, 30, 35, 732
Tsp45I GTSAC 1 cut(s) 1177
TspDTI ATGAA 5 cut(s) 126, 380, 388, 494, 1017
TspGWI ACGGA 1 cut(s) 137
TspRI CASTG 7 cut(s) 69, 243, 717, 790, 802, 859, 889
VneI GTGCAC 1 cut(s) 786
VspI ATTAAT 1 cut(s) 189
XapI RAATTY 2 cut(s) 259, 1247
XceI RCATGY 1 cut(s) 1084
XmaJI CCTAGG 1 cut(s) 803
XmnI GAANNNNTTC 1 cut(s) 177
XspI CTAG 3 cut(s) 93, 804, 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.