Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0005052

Physical Location & Seq

Genomic Coordinates Reverse (-)

33614192 .. 33616973

Gene Structure 2782 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0301100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1500 bp

ATGAATGAGCAAGTGGAAAAAATTGACTTGACAGGTAGCCGAATGTCAGCTGTTCTACAAGCATCATCAGACCCCGCAAAATTGGTCTTGGATGCAATGCAAGGGTTTTATCCGTCAAATCTTACTGTGGAAAACAGAGAGTTTGAGTCACATTATCCTCAGGTGAGAGAAGAAGCAGTGAAATTGGCAGCTGATTGGAAAGAGAAGATGGCAGTTACCGCTGATAATAGTTGGGAGGCTTTGGACTTTTTGCGGCTTATCACTACGTATGAATTGACAAGTATTTGTGATTTGAGGGAGCTTCAAAGTCTTCTTGCTATGGTTTCTCAGCCAGAACAAGCTGCTGAATTAAGTAGGGCACTTGGTATCACATATAAGGCACCTGCAACTATATCTTCACCAAATAATGCTGAGGGACCAGAATCTTCGCTGGCTAGAAATGAACCAACTCCTTCTCCTCCGAATAAATATTTACAGAATGAAAGGATTGTTTCTCTTCCTTCCATTGCCAAGACTGGGGGACCCAAATCTTTTGGGGCTAGATTTGCAGCAACTCTTTCTTCACCAAATAATGCTGAGGGACCAGAATCTTCTCTCACTAGAATTGCAGCAACTCTTTCTTCACCGAATAATGCTTTACAGGAAAATCTGTTGGTCACTCTTAAATCATCATCAGACCCTGCAAAACTTTTGTTGGATTCAATGCAAGGGTCTCTTACGTATTACTGGAGAAATGGAGATGTTAGTTCCAAAGAAAAGGTTGTATCTGGTAACATTACTCTATTGAAGATTCTAATGGGACTGTCAACACATGTTGGACCTCATCTGAAAGAAAATGCAACAAATCTAGCATCCCAGTGGAAAGAAAAGTTGAGAGGTGATACTGAAAATTCATTGGAGAGTTTGGGGTATTTGTTTATAGCTATGTATGAATTGCTTGGAACCTTACATGAAGATGAGATTGTAATGCTTCTTAGAAGGGTTTCGCAGCATAAACAGTCTCTAGAGTTATGTCAGACACATGGTTTTGCAGATTATATCCCTGATTTTATACGGAAGCTTATTGAAAAGAAGTCACTGATGGAGGCTGTTAGATCTATTTGTGCCTTCAAATTATTTGACAAGCTCCCCCCAGTGCCACTCTTAAAAGAATATGTGGATGATGTAATGATGTGTTCCAAACTAATTTGTGGGTCACAGATGATACCTGATGAAAAGGATAAGGCTCTGAATGGTAAAATAGCTGATCTAAGAGCTGCCATTCAATGCATCAAAGATTACGACCTCGAGTCTGAATACCCATCCAAGACTGTTGAATTGCAAATAGTTCAGCTTGAAATGCTGAAGGAAAAATGGAGCAGTTTGGCGCCATCTCTAGTACCCAAAGTTGAACAAGAAGAGAGAGAAAGGAAGAAGCCTTGTACTAGCTCTTCTGCCCCAAAGTTACAACCAGGTAAGGTGCCGAAAATAAAGTTCGTCTTCAGTGGTTCGAGACCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0006810 GO:0006811 GO:0006812 GO:0006820 GO:0006865 GO:0008150 GO:0008324 GO:0008509 GO:0008514 GO:0015075 GO:0015171 GO:0015175 GO:0015179 GO:0015194 GO:0015318 GO:0015711 GO:0015804 GO:0015807 GO:0015825 GO:0015849 GO:0022857 GO:0022889 GO:0032329 GO:0034220 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0098655 GO:0098656 GO:1902475 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Serinc

Protein Analysis

499

Amino Acids

55.37

Weight (kDa)

5.89

Isoelectric Point (pI)

46.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Frigida	PF07899	53 - 131	5.7e-13	Frigida-like protein
Frigida	PF07899	207 - 451	4.7e-48	Frigida-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005052)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16180 AT1G16180
fragaria_vesca	FvH4_6g28080 FvH4_6g28080 FvH4_6g28080 FvH4_6g28080 FvH4_6g28080 FvH4_6g28080
malus_domestica	MD09G1229700.v1.1 MD17G1228400.v1.1
prunus_persica	Prupe.3G105800_v2.0.a1 Prupe.3G105800_v2.0.a1 Prupe.3G105800_v2.0.a1
pyrus_communis	pycom09g14820 pycom17g23100
rosa_chinensis	RchiOBHm_Chr2g0131941
rosa_laevigata	RLG00000019219
rosa_multiflora	Rmu_sc0000584.1_g000008 Rmu_sc0003731.1_g000001
rosa_roxburghii	Rroxscaffold_2G00112470
rosa_rugosa	Rorug02G0300800 Rorug02G0300900 Rorug02G0301000 Rorug02G0301100 Rorug02G0301200 Rorug02G0301300
rosa_samantha	Rh2BG360100 Rh2CG338000
rosa_wichuraiana	Rw2G028690 Rw2G028740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	391
Acc36I	ACCTGC	1 cut(s)	391
AccB1I	GGYRCC	3 cut(s)	379, 1366, 1459
AciI	CCGC	3 cut(s)	75, 219, 253
AcsI	RAATTY	1 cut(s)	889
AcuI	CTGAAG	2 cut(s)	1364, 1465
AcyI	GRCGYC	1 cut(s)	1367
AfaI	GTAC	2 cut(s)	1380, 1423
AfiI	CCNNNNNNNGG	1 cut(s)	516
AflIII	ACRYGT	1 cut(s)	811
AgsI	TTSAA	9 cut(s)	305, 702, 787, 1067, 1111, 1265, 1316, 1337, 1391
AhdI	GACNNNNNGTC	1 cut(s)	1288
AjnI	CCWGG	1 cut(s)	1450
Alw26I	GTCTC	3 cut(s)	717, 1005, 1486
AlwNI	CAGNNNCTG	1 cut(s)	680
Ama87I	CYCGRG	1 cut(s)	1286
ApeKI	GCWGC	6 cut(s)	188, 341, 548, 608, 988, 1256
ApoI	RAATTY	1 cut(s)	889
Asp700I	GAANNNNTTC	1 cut(s)	982
AspLEI	GCGC	1 cut(s)	1369
AspS9I	GGNCC	4 cut(s)	416, 521, 581, 818
AsuHPI	GGTGA	5 cut(s)	175, 390, 555, 615, 890
AvaI	CYCGRG	1 cut(s)	1286
AvaII	GGWCC	4 cut(s)	416, 521, 581, 818
AxyI	CCTNAGG	1 cut(s)	159
BaeGI	GKGCMC	1 cut(s)	361
BanI	GGYRCC	3 cut(s)	379, 1366, 1459
BarI	GAAGNNNNNNTAC	2 cut(s)	1414, 1446
BbsI	GAAGAC	2 cut(s)	302, 1471
BbvCI	CCTCAGC	2 cut(s)	411, 576
BbvI	GCAGC	6 cut(s)	200, 328, 560, 620, 1000, 1243
BccI	CCATC	4 cut(s)	202, 1075, 1309, 1378
BciT130I	CCWGG	1 cut(s)	1452
BcoDI	GTCTC	3 cut(s)	717, 1005, 1486
BfaI	CTAG	7 cut(s)	435, 540, 600, 848, 1004, 1376, 1425
BfoI	RGCGCY	1 cut(s)	1370
BfuAI	ACCTGC	1 cut(s)	391
BglII	AGATCT	1 cut(s)	1094
BisI	GCNGC	7 cut(s)	189, 254, 342, 549, 609, 989, 1257
BlsI	GCNGC	7 cut(s)	190, 255, 343, 550, 610, 990, 1258
Bme1390I	CCNGG	1 cut(s)	1452
Bme18I	GGWCC	4 cut(s)	416, 521, 581, 818
BmeRI	GACNNNNNGTC	1 cut(s)	1288
BmeT110I	CYCGRG	1 cut(s)	1286
BmgT120I	GGNCC	4 cut(s)	416, 521, 581, 818
BmiI	GGNNCC	8 cut(s)	381, 417, 522, 523, 582, 943, 1368, 1461
BmrFI	CCNGG	1 cut(s)	1452
BmrI	ACTGGG	3 cut(s)	525, 850, 1127
BmsI	GCATC	4 cut(s)	71, 82, 860, 1278
BmuI	ACTGGG	3 cut(s)	525, 850, 1127
BpiI	GAAGAC	2 cut(s)	302, 1471
BpmI	CTGGAG	1 cut(s)	748
Bpu10I	CCTNAGC	2 cut(s)	411, 576
BsaAI	YACGTR	2 cut(s)	267, 720
BsaHI	GRCGYC	1 cut(s)	1367
BsaI	GGTCTC	2 cut(s)	717, 1486
BsaXI	ACNNNNNCTCC	2 cut(s)	439, 469
Bsc4I	CCNNNNNNNGG	1 cut(s)	516
Bse1I	ACTGG	4 cut(s)	520, 731, 856, 1133
Bse21I	CCTNAGG	1 cut(s)	159
Bse3DI	GCAATG	2 cut(s)	102, 504
BseBI	CCWGG	1 cut(s)	1452
BseGI	GGATG	4 cut(s)	97, 851, 1165, 1301
BseLI	CCNNNNNNNGG	1 cut(s)	516
BseMI	GCAATG	2 cut(s)	102, 504
BseMII	CTCAG	4 cut(s)	173, 341, 402, 567
BseNI	ACTGG	4 cut(s)	520, 731, 856, 1133
BseRI	GAGGAG	1 cut(s)	447
BseSI	GKGCMC	1 cut(s)	361
BseXI	GCAGC	6 cut(s)	200, 328, 560, 620, 1000, 1243
BshNI	GGYRCC	3 cut(s)	379, 1366, 1459
BsiHKCI	CYCGRG	1 cut(s)	1286
BslFI	GGGAC	4 cut(s)	429, 534, 594, 813
BslI	CCNNNNNNNGG	1 cut(s)	516
BsmAI	GTCTC	3 cut(s)	717, 1005, 1486
BsmFI	GGGAC	4 cut(s)	429, 534, 594, 813
Bso31I	GGTCTC	2 cut(s)	717, 1486
BsoBI	CYCGRG	1 cut(s)	1286
Bsp1286I	GDGCHC	1 cut(s)	361
Bsp143I	GATC	2 cut(s)	1094, 1246
BspACI	CCGC	3 cut(s)	75, 219, 253
BspCNI	CTCAG	4 cut(s)	172, 340, 403, 568
BspLI	GGNNCC	8 cut(s)	381, 417, 522, 523, 582, 943, 1368, 1461
BspMI	ACCTGC	1 cut(s)	391
BspQI	GCTCTTC	1 cut(s)	1435
BspT107I	GGYRCC	3 cut(s)	379, 1366, 1459
BspTNI	GGTCTC	2 cut(s)	717, 1486
BsrDI	GCAATG	2 cut(s)	102, 504
BsrI	ACTGG	4 cut(s)	520, 731, 856, 1133
BssMI	GATC	2 cut(s)	1094, 1246
BssNI	GRCGYC	1 cut(s)	1367
Bst2UI	CCWGG	1 cut(s)	1452
Bst4CI	ACNGT	4 cut(s)	127, 804, 999, 1312
Bst6I	CTCTTC	3 cut(s)	501, 1392, 1435
BstACI	GRCGYC	1 cut(s)	1367
BstBAI	YACGTR	2 cut(s)	267, 720
BstC8I	GCNNGC	1 cut(s)	432
BstDEI	CTNAG	6 cut(s)	159, 327, 411, 576, 974, 1250
BstF5I	GGATG	4 cut(s)	97, 851, 1165, 1301
BstH2I	RGCGCY	1 cut(s)	1370
BstHHI	GCGC	1 cut(s)	1369
BstKTI	GATC	2 cut(s)	1097, 1249
BstMAI	GTCTC	3 cut(s)	717, 1005, 1486
BstMBI	GATC	2 cut(s)	1094, 1246
BstMWI	GCNNNNNNNGC	3 cut(s)	218, 545, 1339
BstNI	CCWGG	1 cut(s)	1452
BstNSI	RCATGY	1 cut(s)	815
BstSCI	CCNGG	1 cut(s)	1450
BstSLI	GKGCMC	1 cut(s)	361
BstSNI	TACGTA	2 cut(s)	267, 720
BstV1I	GCAGC	6 cut(s)	200, 328, 560, 620, 1000, 1243
BstV2I	GAAGAC	2 cut(s)	302, 1471
BstX2I	RGATCY	1 cut(s)	1094
BstYI	RGATCY	1 cut(s)	1094
Bsu36I	CCTNAGG	1 cut(s)	159
BtsCI	GGATG	4 cut(s)	97, 851, 1165, 1301
BtsI	GCAGTG	1 cut(s)	183
BtsIMutI	CAGTG	5 cut(s)	183, 863, 1076, 1140, 1489
BveI	ACCTGC	1 cut(s)	391
Cac8I	GCNNGC	1 cut(s)	432
CaiI	CAGNNNCTG	1 cut(s)	680
CfoI	GCGC	1 cut(s)	1369
Cfr13I	GGNCC	4 cut(s)	416, 521, 581, 818
CsiI	ACCWGGT	1 cut(s)	1450
Csp6I	GTAC	2 cut(s)	1379, 1422
CviAII	CATG	3 cut(s)	812, 950, 1022
CviQI	GTAC	2 cut(s)	1379, 1422
DdeI	CTNAG	6 cut(s)	159, 327, 411, 576, 974, 1250
DinI	GGCGCC	1 cut(s)	1368
DpnI	GATC	2 cut(s)	1096, 1248
DpnII	GATC	2 cut(s)	1094, 1246
DriI	GACNNNNNGTC	1 cut(s)	1288
Eam1104I	CTCTTC	3 cut(s)	501, 1392, 1435
Eam1105I	GACNNNNNGTC	1 cut(s)	1288
EarI	CTCTTC	3 cut(s)	501, 1392, 1435
Eco105I	TACGTA	2 cut(s)	267, 720
Eco31I	GGTCTC	2 cut(s)	717, 1486
Eco47I	GGWCC	4 cut(s)	416, 521, 581, 818
Eco57I	CTGAAG	2 cut(s)	1364, 1465
Eco81I	CCTNAGG	1 cut(s)	159
Eco88I	CYCGRG	1 cut(s)	1286
EcoO109I	RGGNCCY	1 cut(s)	521
EcoRII	CCWGG	1 cut(s)	1450
EcoT22I	ATGCAT	1 cut(s)	1271
EgeI	GGCGCC	1 cut(s)	1368
EheI	GGCGCC	1 cut(s)	1368
FaeI	CATG	3 cut(s)	815, 953, 1025
FalI	AAGNNNNNCTT	4 cut(s)	699, 731, 1463, 1495
FaqI	GGGAC	4 cut(s)	429, 534, 594, 813
FatI	CATG	3 cut(s)	811, 949, 1021
FauI	CCCGC	1 cut(s)	82
Fnu4HI	GCNGC	7 cut(s)	189, 254, 342, 549, 609, 989, 1257
FokI	GGATG	4 cut(s)	104, 838, 1172, 1288
Fsp4HI	GCNGC	7 cut(s)	189, 254, 342, 549, 609, 989, 1257
FspBI	CTAG	7 cut(s)	435, 540, 600, 848, 1004, 1376, 1425
GlaI	GCGC	1 cut(s)	1368
GluI	GCNGC	7 cut(s)	189, 254, 342, 549, 609, 989, 1257
GsuI	CTGGAG	1 cut(s)	748
HaeII	RGCGCY	1 cut(s)	1370
HhaI	GCGC	1 cut(s)	1369
Hin1I	GRCGYC	1 cut(s)	1367
Hin1II	CATG	3 cut(s)	815, 953, 1025
Hin6I	GCGC	1 cut(s)	1367
HinP1I	GCGC	1 cut(s)	1367
HincII	GTYRAC	1 cut(s)	807
HindII	GTYRAC	1 cut(s)	807
HindIII	AAGCTT	1 cut(s)	1058
HinfI	GANTC	6 cut(s)	146, 422, 587, 698, 790, 1289
HphI	GGTGA	5 cut(s)	175, 390, 555, 615, 890
Hpy166II	GTNNAC	1 cut(s)	807
Hpy188I	TCNGA	7 cut(s)	70, 462, 676, 828, 1017, 1230, 1294
Hpy188III	TCNNGA	2 cut(s)	1004, 1491
Hpy8I	GTNNAC	1 cut(s)	807
HpyAV	CCTTC	5 cut(s)	462, 510, 972, 1117, 1339
HpyCH4III	ACNGT	4 cut(s)	127, 804, 999, 1312
HpyCH4IV	ACGT	2 cut(s)	266, 719
HpyF10VI	GCNNNNNNNGC	3 cut(s)	218, 545, 1339
HpyF3I	CTNAG	6 cut(s)	159, 327, 411, 576, 974, 1250
HpySE526I	ACGT	2 cut(s)	266, 719
Hsp92I	GRCGYC	1 cut(s)	1367
Hsp92II	CATG	3 cut(s)	815, 953, 1025
HspAI	GCGC	1 cut(s)	1367
KasI	GGCGCC	1 cut(s)	1366
KflI	GGGWCCC	1 cut(s)	521
Kzo9I	GATC	2 cut(s)	1094, 1246
LguI	GCTCTTC	1 cut(s)	1435
LmnI	GCTCC	3 cut(s)	298, 1131, 1356
Lsp1109I	GCAGC	6 cut(s)	200, 328, 560, 620, 1000, 1243
LweI	GCATC	4 cut(s)	71, 82, 860, 1278
MabI	ACCWGGT	1 cut(s)	1450
MaeI	CTAG	7 cut(s)	435, 540, 600, 848, 1004, 1376, 1425
MaeII	ACGT	2 cut(s)	266, 719
MaeIII	GTNAC	7 cut(s)	147, 214, 655, 770, 1074, 1194, 1443
MalI	GATC	2 cut(s)	1096, 1248
MboI	GATC	2 cut(s)	1094, 1246
MflI	RGATCY	1 cut(s)	1094
MhlI	GDGCHC	1 cut(s)	361
Mly113I	GGCGCC	1 cut(s)	1367
MlyI	GAGTC	2 cut(s)	155, 1298
MmeI	TCCRAC	2 cut(s)	675, 796
Mph1103I	ATGCAT	1 cut(s)	1271
MroXI	GAANNNNTTC	1 cut(s)	982
MseI	TTAA	4 cut(s)	350, 663, 1145, 1498
MslI	CAYNNNNRTG	2 cut(s)	856, 954
MspA1I	CMGCKG	3 cut(s)	50, 191, 221
MspR9I	CCNGG	1 cut(s)	1452
MvaI	CCWGG	1 cut(s)	1452
MwoI	GCNNNNNNNGC	3 cut(s)	218, 545, 1339
NarI	GGCGCC	1 cut(s)	1367
NdeII	GATC	2 cut(s)	1094, 1246
NlaIII	CATG	3 cut(s)	815, 953, 1025
NlaIV	GGNNCC	8 cut(s)	381, 417, 522, 523, 582, 943, 1368, 1461
NmuCI	GTSAC	4 cut(s)	147, 655, 1074, 1194
NsiI	ATGCAT	1 cut(s)	1271
NspI	RCATGY	1 cut(s)	815
PaeR7I	CTCGAG	1 cut(s)	1286
PaqCI	CACCTGC	1 cut(s)	391
PciI	ACATGT	1 cut(s)	811
PciSI	GCTCTTC	1 cut(s)	1435
PdmI	GAANNNNTTC	1 cut(s)	982
PfeI	GAWTC	4 cut(s)	422, 587, 698, 790
PkrI	GCNGC	7 cut(s)	190, 255, 343, 550, 610, 990, 1258
PleI	GAGTC	2 cut(s)	154, 1297
PluTI	GGCGCC	1 cut(s)	1370
PpsI	GAGTC	2 cut(s)	154, 1297
Ppu21I	YACGTR	2 cut(s)	267, 720
PpuMI	RGGWCCY	1 cut(s)	521
PscI	ACATGT	1 cut(s)	811
Psp5II	RGGWCCY	1 cut(s)	521
Psp6I	CCWGG	1 cut(s)	1450
PspGI	CCWGG	1 cut(s)	1450
PspN4I	GGNNCC	8 cut(s)	381, 417, 522, 523, 582, 943, 1368, 1461
PspPI	GGNCC	4 cut(s)	416, 521, 581, 818
PspPPI	RGGWCCY	1 cut(s)	521
PspXI	VCTCGAGB	1 cut(s)	1286
PstNI	CAGNNNCTG	1 cut(s)	680
PsuI	RGATCY	1 cut(s)	1094
PvuII	CAGCTG	2 cut(s)	50, 191
RsaI	GTAC	2 cut(s)	1380, 1423
RsaNI	GTAC	2 cut(s)	1379, 1422
RseI	CAYNNNNRTG	2 cut(s)	856, 954
SapI	GCTCTTC	1 cut(s)	1435
SaqAI	TTAA	4 cut(s)	350, 663, 1145, 1498
SatI	GCNGC	7 cut(s)	189, 254, 342, 549, 609, 989, 1257
Sau3AI	GATC	2 cut(s)	1094, 1246
Sau96I	GGNCC	4 cut(s)	416, 521, 581, 818
SchI	GAGTC	2 cut(s)	155, 1298
ScrFI	CCNGG	1 cut(s)	1452
SduI	GDGCHC	1 cut(s)	361
SexAI	ACCWGGT	1 cut(s)	1450
SfaNI	GCATC	4 cut(s)	71, 82, 860, 1278
SfoI	GGCGCC	1 cut(s)	1368
Sfr274I	CTCGAG	1 cut(s)	1286
SinI	GGWCC	4 cut(s)	416, 521, 581, 818
SlaI	CTCGAG	1 cut(s)	1286
SmiMI	CAYNNNNRTG	2 cut(s)	856, 954
SmlI	CTYRAG	1 cut(s)	1286
SmoI	CTYRAG	1 cut(s)	1286
SnaBI	TACGTA	2 cut(s)	267, 720
SsiI	CCGC	3 cut(s)	75, 219, 253
SspDI	GGCGCC	1 cut(s)	1366
SspI	AATATT	1 cut(s)	470
SspMI	CTAG	7 cut(s)	435, 540, 600, 848, 1004, 1376, 1425
StyD4I	CCNGG	1 cut(s)	1450
TaaI	ACNGT	4 cut(s)	127, 804, 999, 1312
TaiI	ACGT	2 cut(s)	269, 722
TaqI	TCGA	2 cut(s)	1287, 1490
TatI	WGTACW	1 cut(s)	1421
TauI	GCSGC	1 cut(s)	256
TfiI	GAWTC	4 cut(s)	422, 587, 698, 790
Tru1I	TTAA	4 cut(s)	350, 663, 1145, 1498
Tru9I	TTAA	4 cut(s)	350, 663, 1145, 1498
TscAI	CASTG	5 cut(s)	183, 863, 1083, 1140, 1489
TseFI	GTSAC	4 cut(s)	147, 655, 1074, 1194
TseI	GCWGC	6 cut(s)	188, 341, 548, 608, 988, 1256
Tsp45I	GTSAC	4 cut(s)	147, 655, 1074, 1194
TspDTI	ATGAA	8 cut(s)	17, 285, 456, 495, 882, 945, 966, 1227
TspGWI	ACGGA	2 cut(s)	102, 1069
TspRI	CASTG	5 cut(s)	183, 863, 1083, 1140, 1489
VpaK11BI	GGWCC	4 cut(s)	416, 521, 581, 818
XapI	RAATTY	1 cut(s)	889
XbaI	TCTAGA	1 cut(s)	1003
XceI	RCATGY	1 cut(s)	815
XhoI	CTCGAG	1 cut(s)	1286
XmnI	GAANNNNTTC	1 cut(s)	982
XspI	CTAG	7 cut(s)	435, 540, 600, 848, 1004, 1376, 1425
Zsp2I	ATGCAT	1 cut(s)	1271

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.