Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002700

Physical Location & Seq

Genomic Coordinates Reverse (-)

29506566 .. 29510529

Gene Structure 3964 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1234800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2565 bp

ATGTCGCAGTCATACATAAATCTTTTGGATTTGGCATCAGGGAATTTCCCTACCATGGAGGGGAAAAGAAGACGGTTCCCAAGGGTGAACACAGCTCCTGGAAATTTGTCCGATCTTGACGATGATCAAGCTCGTAGTGTGTCGTCTGATCAACCTTCCTCAATTGCTTCCGATCGGGTGATCATTGTAGCTAACCAACTTCCTGTGAAAGCAAAGAGAAGGGAGGATAATAAAGGTTGGACTTTCAGTTGGAATGAGGATTCTTTGCTTTTGCAGCTTAAGGATGGACTGCCAGAAGACATGGAGGTTCTTTATGTTGGCTCGTTGAAGGTTTCCGTTGATCCTAAAGAGCAGGATGATGTGTCACAGGTCTTACTGGAGAGATTCAAATGTGTTCCGACTTTTCTACCCCCTGACATTCTGTCAAAATTCTATGATGGTTTCTGCAAGAGGCATTTATGGCCACTTTTTCATTACATGCTGCCGTTTTCAGCTGATCAAGGAGGCCGATTTGATCGGTCTTTGTGGGAAGCTTACATATCAGCTAATAAGTTGTTTTTTCAAAGAGTGGTTGAGCTTATAAACCCGGATGAAGATTATATCTGGATTCATGATTACCATTTGATGGTGCTGCCAACTTTCCTGAGAAGGCGTTTTAATCGAGTTAGGATGGGCTTTTTCCTTCACAGTCCATTTCCCTCGTCTGAGATATATAGAACTCTTCCTGTAAGAGAAGAAATACTTAAGGCTTTACTCAATGCAGATATTATTGGGTTCCACACCTTTGATTATGCTCGTCATTTTCTGACATGTTGCAGTCGGATGTTGGGCTTGGTATATCAGTCAAAGAGGGGTTACTTAGGATTGGAATATTATGGAAGGAACATCAGGATCAAGATTATGCCAGTTGGGGTTCATATGGGTTGGATAGAATCGGTGATGAAGGTGGCGGATGACGAGAGTAGAGTGAGGGAACTTACACAACAGTTTGAAGGGAAAACAATGTTGCTTGGTATAGATGATATGGACATTTTTAAAGGAATTAATTTGAAAATTCTAGCAATGGAACAGATGCTGAAGCAACATCCAAATTGGCAGGGAAAGGCAGTGCTGGTCCAGATTCTAAACCCTGTGAGAGGTAAAGGGATAGATTTGGAGGAAATACGAGCAGAAATACAAGAAGGCTGCAGGCGGATCAATGCAGAATTTGGTCAACCAGGCTATGAGCCAATAATCATTATTGACAGGCCAGTTTCCATTAACGAGAAGATTTGCTATTACAACATTGCTGAGTGTGTCGTTGTGACAGCCGTTAGAGATGGGATGAACCTTACTCCATATGAATATGTAGTTTGCAGACAAGGAATATCTGGTTCTAATTCGTGTTCAAATTTCGGTGGTCCAAAGAAGAGCATGTTAGTGGTGTCAGAATTTATTGGATGTTCTCCATCACTCAGTGGGGCAATCCGTGTCAATCCATGGAATGTTGAAACAACTGGGGAGGCACTGAATGTGGCGATTTCAGTGCTTGATTCAGAGAAAGAACTGCGACATGAGAAGCATTATCGGTATGTTAGTACACACGATGTGGCTTATTGGTCGCGCAGTTTCTTGCAAGACATGCAGAGAGCTTGTGCAGACCATTTTAAGAGAAGATGTTGGGGAATTGGTTTTGGCTTTGGATTCAGAGTTGTGGCACTTGATCCTAATTTCAGAAAACTTTCAATTGATGCTATTGTTTCGGCTTACAGAGGGGCACAAAATAGGGCCATTCTGTTGGACTACGATGGTACTGTGATGCCCCAGAACTCAATTGACAAGTCCCCTAGTCAGAAGGTAATCTCAATTATGAACACACTTTGTGTGGACCCGAAAAACACAGTTTTCATTGTCACTGGCCGAGGGAGGGAGTGCTTAAGCAAGTGGTTTTCTCCCTGTCAGAGGATGGGAATTGCGGCTGAGCATGGTTACTTCTTAAGATGGTCACAAAATCAGGAATGGGAAACCTGTCTTCAGGGTTTTGAGTTTGGCTGGATGAAGACTGTTGAACCCGTGATGCAATTGTATACGGAGGCTACTGATGGTTCTTCTATTGAAAGAAAGGAAAGTGCTTTGGTTTGGCAATATCGTGACGCAGACCCTGGTTTCGGGTCCTCCCAGGCAAAGGAGATGTTGGATCATCTAGAGAGTGTCTTGGCAAATGAACCTGTTGCTGTCAAGAACGGTCAGTTCATTGTAGAAGTAAAGCCTCAGGGAGTTAGTAAAGGACATGTAGCAGAGAAGATTTTCTCATCAATGGCGGAAAATGGAAAACACGCAGACTTTGTGCTCTGTATTGGCGATGACAGATCCGATGAGGACATGTTTGAGATATTTGGTAACTCGATGCAGAAGAAAGTTCTCTCTCCAAATCCTTCGGTTTTTGCTTGCACGGTTGGGCAAAAGCCTAGCAAGGCTACATATTATTTGGACGATACAGCGGAAGTGACAAACATGCTTGAATCTCTTTCTGAAGCGTTTGAGTCTTTGCCACCCACTGAAGAAGGATACGAAAGCTCTCCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0033554 GO:0034637 GO:0042578 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044424 GO:0044444 GO:0044464 GO:0046351 GO:0050896 GO:0051716 GO:0070413 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

855

Amino Acids

97.09

Weight (kDa)

5.77

Isoelectric Point (pI)

47.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_20	PF00982	59 - 542	5e-173	Glycosyltransferase family 20
Trehalose_PPase	PF02358	592 - 826	2.7e-79	Trehalose-phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002700)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06410 AT1G06410 AT1G06410
fragaria_vesca	FvH4_3g07260 FvH4_6g27760 FvH4_6g27760
malus_domestica	MD00G1008700.v1.1 MD09G1234800.v1.1 MD10G1275100.v1.1
prunus_persica	Prupe.3G113100_v2.0.a1 Prupe.3G113100_v2.0.a1 Prupe.3G113100_v2.0.a1 Prupe.3G113100_v2.0.a1 Prupe.3G113100_v2.0.a1 Prupe.4G067300_v2.0.a1
pyrus_communis	pycom05g27390 pycom09g15300 pycom10g22970
rosa_chinensis	RchiOBHm_Chr2g0131021 RchiOBHm_Chr5g0011801
rosa_laevigata	RLG00000019161 RLG00000031862
rosa_multiflora	Rmu_sc0003629.1_g000054 Rmu_ssc0000357.1_g000005
rosa_roxburghii	Rroxscaffold_1G00064760
rosa_rugosa	Rorug02G0294400 Rorug04G0459400
rosa_samantha	Rh2AG346700 Rh2BG354100 Rh2CG332700 Rh2DG372200 Rh5AG090700 Rh5BG086300 Rh5CG100100 Rh5DG086400
rosa_wichuraiana	Rw0G018970 Rw2G028110 Rw2G028150 Rw5G007920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	581
AccB7I	CCANNNNNTGG	1 cut(s)	625
AccI	GTMKAC	1 cut(s)	2066
AccII	CGCG	1 cut(s)	1603
AciI	CCGC	5 cut(s)	950, 1192, 1955, 2300, 2480
AclWI	GGATC	6 cut(s)	335, 899, 1202, 1697, 2184, 2343
AcoI	YGGCCR	2 cut(s)	461, 1897
AcsI	RAATTY	7 cut(s)	43, 103, 428, 1053, 1205, 1390, 1430
AcuI	CTGAAG	4 cut(s)	1097, 1997, 2532, 2559
AdeI	CACNNNGTG	3 cut(s)	1457, 1588, 1862
AfaI	GTAC	2 cut(s)	1579, 1792
AfiI	CCNNNNNNNGG	8 cut(s)	55, 60, 625, 1136, 1906, 1941, 2147, 2164
AflII	CTTAAG	4 cut(s)	278, 743, 1915, 1975
AflIII	ACRYGT	3 cut(s)	809, 2269, 2361
AhdI	GACNNNNNGTC	1 cut(s)	421
AjnI	CCWGG	4 cut(s)	97, 1216, 2140, 2157
Alw21I	GWGCWC	1 cut(s)	2331
AlwI	GGATC	6 cut(s)	335, 899, 1202, 1697, 2184, 2343
AlwNI	CAGNNNCTG	3 cut(s)	98, 1075, 2141
AoxI	GGCC	5 cut(s)	461, 505, 1247, 1767, 1897
ApeKI	GCWGC	4 cut(s)	274, 481, 631, 1185
ApoI	RAATTY	7 cut(s)	43, 103, 428, 1053, 1205, 1390, 1430
ArsI	GACNNNNNNTTYG	2 cut(s)	2129, 2161
AseI	ATTAAT	1 cut(s)	1044
Asp700I	GAANNNNTTC	2 cut(s)	1720, 2285
AspLEI	GCGC	1 cut(s)	1605
AspS9I	GGNCC	5 cut(s)	1114, 1400, 1767, 1867, 2151
AsuC2I	CCSGG	1 cut(s)	587
AsuHPI	GGTGA	3 cut(s)	97, 190, 949
AvaII	GGWCC	4 cut(s)	1114, 1400, 1867, 2151
AxyI	CCTNAGG	1 cut(s)	2250
BaeGI	GKGCMC	1 cut(s)	1759
BalI	TGGCCA	1 cut(s)	463
BbsI	GAAGAC	4 cut(s)	76, 303, 2003, 2045
Bbv12I	GWGCWC	1 cut(s)	2331
BbvI	GCAGC	4 cut(s)	286, 468, 618, 1172
BceAI	ACGGC	2 cut(s)	469, 1295
BcgI	CGANNNNNNTGC	2 cut(s)	1507, 1541
BciT130I	CCWGG	4 cut(s)	99, 1218, 2142, 2159
BciVI	GTATCC	1 cut(s)	2540
BclI	TGATCA	4 cut(s)	124, 148, 180, 496
BcnI	CCSGG	1 cut(s)	587
BfaI	CTAG	4 cut(s)	1058, 1827, 2183, 2448
BfmI	CTRYAG	1 cut(s)	1186
BfrI	CTTAAG	4 cut(s)	278, 743, 1915, 1975
BfuI	GTATCC	1 cut(s)	2540
BisI	GCNGC	5 cut(s)	275, 482, 632, 1186, 1956
BlpI	GCTNAGC	1 cut(s)	1959
BlsI	GCNGC	5 cut(s)	276, 483, 633, 1187, 1957
Bme1390I	CCNGG	5 cut(s)	99, 587, 1218, 2142, 2159
Bme18I	GGWCC	4 cut(s)	1114, 1400, 1867, 2151
BmeRI	GACNNNNNGTC	1 cut(s)	421
BmgT120I	GGNCC	5 cut(s)	1114, 1400, 1767, 1867, 2151
BmiI	GGNNCC	4 cut(s)	77, 776, 1869, 2152
BmrFI	CCNGG	5 cut(s)	99, 587, 1218, 2142, 2159
BmrI	ACTGGG	1 cut(s)	1506
BmsI	GCATC	6 cut(s)	44, 1062, 1720, 1788, 2046, 2376
BmuI	ACTGGG	1 cut(s)	1506
BpiI	GAAGAC	4 cut(s)	76, 303, 2003, 2045
BpmI	CTGGAG	1 cut(s)	398
Bpu1102I	GCTNAGC	1 cut(s)	1959
BpuMI	CCSGG	1 cut(s)	587
BsaJI	CCNNGG	6 cut(s)	54, 80, 1478, 1900, 2140, 2157
Bsc4I	CCNNNNNNNGG	8 cut(s)	55, 60, 625, 1136, 1906, 1941, 2147, 2164
Bse1I	ACTGG	5 cut(s)	381, 905, 1250, 1501, 1900
Bse21I	CCTNAGG	1 cut(s)	2250
Bse3DI	GCAATG	2 cut(s)	1068, 1284
BseBI	CCWGG	4 cut(s)	99, 1218, 2142, 2159
BseDI	CCNNGG	6 cut(s)	54, 80, 1478, 1900, 2140, 2157
BseLI	CCNNNNNNNGG	8 cut(s)	55, 60, 625, 1136, 1906, 1941, 2147, 2164
BseMI	GCAATG	2 cut(s)	1068, 1284
BseMII	CTCAG	6 cut(s)	635, 696, 1281, 1468, 1950, 2264
BseNI	ACTGG	5 cut(s)	381, 905, 1250, 1501, 1900
BseSI	GKGCMC	1 cut(s)	1759
BseXI	GCAGC	4 cut(s)	286, 468, 618, 1172
BsgI	GTGCAG	1 cut(s)	1656
Bsh1236I	CGCG	1 cut(s)	1603
Bsh1285I	CGRYCG	1 cut(s)	175
BshFI	GGCC	5 cut(s)	463, 507, 1249, 1769, 1899
BsiEI	CGRYCG	1 cut(s)	175
BsiHKAI	GWGCWC	1 cut(s)	2331
BsiSI	CCGG	1 cut(s)	587
BslFI	GGGAC	1 cut(s)	1807
BslI	CCNNNNNNNGG	8 cut(s)	55, 60, 625, 1136, 1906, 1941, 2147, 2164
BsmFI	GGGAC	1 cut(s)	1807
BsnI	GGCC	5 cut(s)	463, 507, 1249, 1769, 1899
Bsp1286I	GDGCHC	2 cut(s)	1759, 2331
Bsp1720I	GCTNAGC	1 cut(s)	1959
Bsp19I	CCATGG	2 cut(s)	54, 1478
BspACI	CCGC	5 cut(s)	950, 1192, 1955, 2300, 2480
BspANI	GGCC	5 cut(s)	463, 507, 1249, 1769, 1899
BspCNI	CTCAG	6 cut(s)	636, 697, 1282, 1467, 1951, 2263
BspFNI	CGCG	1 cut(s)	1603
BspHI	TCATGA	1 cut(s)	610
BspLI	GGNNCC	4 cut(s)	77, 776, 1869, 2152
BspMAI	CTGCAG	1 cut(s)	1190
BspPI	GGATC	6 cut(s)	335, 899, 1202, 1697, 2184, 2343
BspQI	GCTCTTC	1 cut(s)	1403
BspTI	CTTAAG	4 cut(s)	278, 743, 1915, 1975
BsrDI	GCAATG	2 cut(s)	1068, 1284
BsrI	ACTGG	5 cut(s)	381, 905, 1250, 1501, 1900
BssECI	CCNNGG	6 cut(s)	54, 80, 1478, 1900, 2140, 2157
BssNAI	GTATAC	1 cut(s)	2067
BssT1I	CCWWGG	3 cut(s)	54, 80, 1478
Bst1107I	GTATAC	1 cut(s)	2067
Bst2UI	CCWGG	4 cut(s)	99, 1218, 2142, 2159
Bst4CI	ACNGT	8 cut(s)	75, 689, 987, 1795, 1882, 2044, 2225, 2434
Bst6I	CTCTTC	2 cut(s)	726, 1403
BstAFI	CTTAAG	4 cut(s)	278, 743, 1915, 1975
BstAPI	GCANNNNNTGC	1 cut(s)	1621
BstC8I	GCNNGC	2 cut(s)	1190, 2428
BstDEI	CTNAG	7 cut(s)	644, 705, 859, 1290, 1454, 1959, 2250
BstDSI	CCRYGG	2 cut(s)	54, 1478
BstENI	CCTNNNNNAGG	2 cut(s)	1134, 1939
BstFNI	CGCG	1 cut(s)	1603
BstHHI	GCGC	1 cut(s)	1605
BstMCI	CGRYCG	1 cut(s)	175
BstMWI	GCNNNNNNNGC	3 cut(s)	274, 460, 1621
BstNI	CCWGG	4 cut(s)	99, 1218, 2142, 2159
BstNSI	RCATGY	7 cut(s)	481, 813, 1417, 1624, 2273, 2365, 2497
BstSCI	CCNGG	5 cut(s)	97, 585, 1216, 2140, 2157
BstSFI	CTRYAG	1 cut(s)	1186
BstSLI	GKGCMC	1 cut(s)	1759
BstUI	CGCG	1 cut(s)	1603
BstV1I	GCAGC	4 cut(s)	286, 468, 618, 1172
BstV2I	GAAGAC	4 cut(s)	76, 303, 2003, 2045
BstX2I	RGATCY	1 cut(s)	2348
BstXI	CCANNNNNNTGG	1 cut(s)	1777
BstYI	RGATCY	1 cut(s)	2348
BstZ17I	GTATAC	1 cut(s)	2067
Bsu36I	CCTNAGG	1 cut(s)	2250
BsuI	GTATCC	1 cut(s)	2540
BsuRI	GGCC	5 cut(s)	463, 507, 1249, 1769, 1899
BtgI	CCRYGG	2 cut(s)	54, 1478
BtgZI	GCGATG	1 cut(s)	2355
BtsI	GCAGTG	1 cut(s)	1113
BtsIMutI	CAGTG	6 cut(s)	1113, 1462, 1505, 1530, 1893, 2535
Cac8I	GCNNGC	2 cut(s)	1190, 2428
CaiI	CAGNNNCTG	3 cut(s)	98, 1075, 2141
CciI	TCATGA	1 cut(s)	610
CfoI	GCGC	1 cut(s)	1605
Cfr13I	GGNCC	5 cut(s)	1114, 1400, 1767, 1867, 2151
CseI	GACGC	1 cut(s)	2141
Csp6I	GTAC	2 cut(s)	1578, 1791
CviQI	GTAC	2 cut(s)	1578, 1791
DdeI	CTNAG	7 cut(s)	644, 705, 859, 1290, 1454, 1959, 2250
DraI	TTTAAA	1 cut(s)	1036
DraIII	CACNNNGTG	3 cut(s)	1457, 1588, 1862
DriI	GACNNNNNGTC	1 cut(s)	421
EaeI	YGGCCR	2 cut(s)	461, 1897
Eam1104I	CTCTTC	2 cut(s)	726, 1403
Eam1105I	GACNNNNNGTC	1 cut(s)	421
EarI	CTCTTC	2 cut(s)	726, 1403
EciI	GGCGGA	3 cut(s)	965, 1207, 2315
Eco130I	CCWWGG	3 cut(s)	54, 80, 1478
Eco47I	GGWCC	4 cut(s)	1114, 1400, 1867, 2151
Eco57I	CTGAAG	4 cut(s)	1097, 1997, 2532, 2559
Eco81I	CCTNAGG	1 cut(s)	2250
EcoNI	CCTNNNNNAGG	2 cut(s)	1134, 1939
EcoO109I	RGGNCCY	1 cut(s)	2151
EcoRII	CCWGG	4 cut(s)	97, 1216, 2140, 2157
EcoT14I	CCWWGG	3 cut(s)	54, 80, 1478
ErhI	CCWWGG	3 cut(s)	54, 80, 1478
FalI	AAGNNNNNCTT	2 cut(s)	726, 758
FaqI	GGGAC	1 cut(s)	1807
FauNDI	CATATG	2 cut(s)	918, 1339
FbaI	TGATCA	4 cut(s)	124, 148, 180, 496
FblI	GTMKAC	1 cut(s)	2066
Fnu4HI	GCNGC	5 cut(s)	275, 482, 632, 1186, 1956
Fsp4HI	GCNGC	5 cut(s)	275, 482, 632, 1186, 1956
FspBI	CTAG	4 cut(s)	1058, 1827, 2183, 2448
GlaI	GCGC	1 cut(s)	1604
GluI	GCNGC	5 cut(s)	275, 482, 632, 1186, 1956
GsuI	CTGGAG	1 cut(s)	398
HaeIII	GGCC	5 cut(s)	463, 507, 1249, 1769, 1899
HapII	CCGG	1 cut(s)	587
HgaI	GACGC	1 cut(s)	2141
HhaI	GCGC	1 cut(s)	1605
Hin6I	GCGC	1 cut(s)	1603
HinP1I	GCGC	1 cut(s)	1603
HincII	GTYRAC	1 cut(s)	1214
HindII	GTYRAC	1 cut(s)	1214
HindIII	AAGCTT	1 cut(s)	531
HinfI	GANTC	9 cut(s)	260, 384, 607, 932, 1120, 1532, 1683, 2501, 2522
HpaII	CCGG	1 cut(s)	587
HphI	GGTGA	3 cut(s)	97, 190, 949
Hpy166II	GTNNAC	5 cut(s)	88, 1214, 1580, 1867, 2067
Hpy8I	GTNNAC	5 cut(s)	88, 1214, 1580, 1867, 2067
HpyCH4III	ACNGT	8 cut(s)	75, 689, 987, 1795, 1882, 2044, 2225, 2434
HpyF10VI	GCNNNNNNNGC	3 cut(s)	274, 460, 1621
HpyF3I	CTNAG	7 cut(s)	644, 705, 859, 1290, 1454, 1959, 2250
HspAI	GCGC	1 cut(s)	1603
Ksp22I	TGATCA	4 cut(s)	124, 148, 180, 496
LguI	GCTCTTC	1 cut(s)	1403
LmnI	GCTCC	1 cut(s)	100
Lsp1109I	GCAGC	4 cut(s)	286, 468, 618, 1172
LweI	GCATC	6 cut(s)	44, 1062, 1720, 1788, 2046, 2376
MaeI	CTAG	4 cut(s)	1058, 1827, 2183, 2448
MaeIII	GTNAC	9 cut(s)	363, 854, 1303, 1891, 1967, 1983, 2129, 2378, 2485
MfeI	CAATTG	4 cut(s)	162, 1725, 1812, 2060
MflI	RGATCY	1 cut(s)	2348
MhlI	GDGCHC	2 cut(s)	1759, 2331
MlsI	TGGCCA	1 cut(s)	463
MluNI	TGGCCA	1 cut(s)	463
MlyI	GAGTC	1 cut(s)	2531
MmeI	TCCRAC	7 cut(s)	218, 230, 422, 800, 905, 1758, 2154
Mox20I	TGGCCA	1 cut(s)	463
MroXI	GAANNNNTTC	2 cut(s)	1720, 2285
MscI	TGGCCA	1 cut(s)	463
MseI	TTAA	9 cut(s)	279, 657, 744, 1035, 1044, 1260, 1647, 1916, 1976
MslI	CAYNNNNRTG	1 cut(s)	1418
Msp20I	TGGCCA	1 cut(s)	463
MspA1I	CMGCKG	2 cut(s)	494, 2480
MspCI	CTTAAG	4 cut(s)	278, 743, 1915, 1975
MspI	CCGG	1 cut(s)	587
MspR9I	CCNGG	5 cut(s)	99, 587, 1218, 2142, 2159
MunI	CAATTG	4 cut(s)	162, 1725, 1812, 2060
MvaI	CCWGG	4 cut(s)	99, 1218, 2142, 2159
MvnI	CGCG	1 cut(s)	1603
MwoI	GCNNNNNNNGC	3 cut(s)	274, 460, 1621
NciI	CCSGG	1 cut(s)	587
NcoI	CCATGG	2 cut(s)	54, 1478
NdeI	CATATG	2 cut(s)	918, 1339
NlaIV	GGNNCC	4 cut(s)	77, 776, 1869, 2152
NmeAIII	GCCGAG	1 cut(s)	1925
NmuCI	GTSAC	6 cut(s)	363, 1303, 1891, 1983, 2129, 2485
NspI	RCATGY	7 cut(s)	481, 813, 1417, 1624, 2273, 2365, 2497
PagI	TCATGA	1 cut(s)	610
PciI	ACATGT	3 cut(s)	809, 2269, 2361
PciSI	GCTCTTC	1 cut(s)	1403
PdmI	GAANNNNTTC	2 cut(s)	1720, 2285
PfeI	GAWTC	8 cut(s)	260, 384, 607, 932, 1120, 1532, 1683, 2501
PflMI	CCANNNNNTGG	1 cut(s)	625
PfoI	TCCNGGA	1 cut(s)	97
PkrI	GCNGC	5 cut(s)	276, 483, 633, 1187, 1957
Ple19I	CGATCG	1 cut(s)	175
PleI	GAGTC	1 cut(s)	2530
PpsI	GAGTC	1 cut(s)	2530
PpuMI	RGGWCCY	1 cut(s)	2151
PscI	ACATGT	3 cut(s)	809, 2269, 2361
PshBI	ATTAAT	1 cut(s)	1044
PsiI	TTATAA	1 cut(s)	581
Psp5II	RGGWCCY	1 cut(s)	2151
Psp6I	CCWGG	4 cut(s)	97, 1216, 2140, 2157
PspGI	CCWGG	4 cut(s)	97, 1216, 2140, 2157
PspN4I	GGNNCC	4 cut(s)	77, 776, 1869, 2152
PspPI	GGNCC	5 cut(s)	1114, 1400, 1767, 1867, 2151
PspPPI	RGGWCCY	1 cut(s)	2151
PstI	CTGCAG	1 cut(s)	1190
PstNI	CAGNNNCTG	3 cut(s)	98, 1075, 2141
PsuI	RGATCY	1 cut(s)	2348
PvuI	CGATCG	1 cut(s)	175
PvuII	CAGCTG	1 cut(s)	494
RsaI	GTAC	2 cut(s)	1579, 1792
RsaNI	GTAC	2 cut(s)	1578, 1791
RseI	CAYNNNNRTG	1 cut(s)	1418
SapI	GCTCTTC	1 cut(s)	1403
SaqAI	TTAA	9 cut(s)	279, 657, 744, 1035, 1044, 1260, 1647, 1916, 1976
SatI	GCNGC	5 cut(s)	275, 482, 632, 1186, 1956
Sau96I	GGNCC	5 cut(s)	1114, 1400, 1767, 1867, 2151
SchI	GAGTC	1 cut(s)	2531
ScrFI	CCNGG	5 cut(s)	99, 587, 1218, 2142, 2159
SduI	GDGCHC	2 cut(s)	1759, 2331
SfaNI	GCATC	6 cut(s)	44, 1062, 1720, 1788, 2046, 2376
SfcI	CTRYAG	1 cut(s)	1186
SinI	GGWCC	4 cut(s)	1114, 1400, 1867, 2151
SmiMI	CAYNNNNRTG	1 cut(s)	1418
SmlI	CTYRAG	4 cut(s)	278, 743, 1915, 1975
SmoI	CTYRAG	4 cut(s)	278, 743, 1915, 1975
SsiI	CCGC	5 cut(s)	950, 1192, 1955, 2300, 2480
SspI	AATATT	1 cut(s)	872
SspMI	CTAG	4 cut(s)	1058, 1827, 2183, 2448
StyD4I	CCNGG	5 cut(s)	97, 585, 1216, 2140, 2157
StyI	CCWWGG	3 cut(s)	54, 80, 1478
TaaI	ACNGT	8 cut(s)	75, 689, 987, 1795, 1882, 2044, 2225, 2434
TaqI	TCGA	2 cut(s)	661, 2384
TaqII	GACCGA	1 cut(s)	507
TatI	WGTACW	1 cut(s)	1577
TauI	GCSGC	1 cut(s)	1958
TfiI	GAWTC	8 cut(s)	260, 384, 607, 932, 1120, 1532, 1683, 2501
Tru1I	TTAA	9 cut(s)	279, 657, 744, 1035, 1044, 1260, 1647, 1916, 1976
Tru9I	TTAA	9 cut(s)	279, 657, 744, 1035, 1044, 1260, 1647, 1916, 1976
TscAI	CASTG	6 cut(s)	1113, 1462, 1512, 1530, 1900, 2542
TseFI	GTSAC	6 cut(s)	363, 1303, 1891, 1983, 2129, 2485
TseI	GCWGC	4 cut(s)	274, 481, 631, 1185
Tsp45I	GTSAC	6 cut(s)	363, 1303, 1891, 1983, 2129, 2485
TspGWI	ACGGA	4 cut(s)	325, 1457, 2084, 2550
TspRI	CASTG	6 cut(s)	1113, 1462, 1512, 1530, 1900, 2542
Van91I	CCANNNNNTGG	1 cut(s)	625
Vha464I	CTTAAG	4 cut(s)	278, 743, 1915, 1975
VpaK11BI	GGWCC	4 cut(s)	1114, 1400, 1867, 2151
VspI	ATTAAT	1 cut(s)	1044
XagI	CCTNNNNNAGG	2 cut(s)	1134, 1939
XapI	RAATTY	7 cut(s)	43, 103, 428, 1053, 1205, 1390, 1430
XbaI	TCTAGA	1 cut(s)	2182
XceI	RCATGY	7 cut(s)	481, 813, 1417, 1624, 2273, 2365, 2497
XmiI	GTMKAC	1 cut(s)	2066
XmnI	GAANNNNTTC	2 cut(s)	1720, 2285
XspI	CTAG	4 cut(s)	1058, 1827, 2183, 2448

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.