Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002700

Physical Location & Seq

Genomic Coordinates Reverse (-)

36650650 .. 36654935

Gene Structure 4286 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1275100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2565 bp

ATGATGTCCAAATCGTATACCAATCTTTTAGATCTTGCTTCCGGAAACTTTCCGGTAATGGGTCGCGAAAGACGGCGGCTTCCGCGTGTAATGACCGTCGCTGGTGTTATATCGGAGCTCGATGATGATCAGGCGGGTAGTGTGTCGTCGGATGTTCCGTCGTCGATCATTCAGGACCGGATAATTATTGTTGCGAATCAGCTCCCTGTGAAAGCTAAGCGGAGACCGGATAACAAAGGATGGAGCTTTAGTTGGGATGAGGACTCATTGGTATTGCAGTTGAAGGACGGTCTACCGGAAGATATGGAGGTTTTGTATGTAGGTTCTTTGAGCGTCGAAGTGGATTCCAATGAACAAGACGATGTATCAGCACTCCTGTTGGATAGATTCAAGTGCGTTCCGGCCTTTTTGCCGCCCGACATTTTGTCCAAGTTCTATCATGGGTTTTGCAAGCAGCATTTGTGGCCTCTGTTTCATTACATGCTTCCTTTCTCAGCAACTCATGGGGGGCGGTTCGATCGGTCTTTGTGGGAGGCGTATGTGGCCGCAAATAAGATTTTCTCGCAAAGGGTGATCGAGGTGATAAATCCGGAGGATGACTATGTGTGGATTCATGATTACCATCTAATGGTGCTGCCTACCTTCTTGAGGAGGATGTTCAATAGATTGAGAATGGGGTTTTTTCTCCATAGCCCATTCCCTTCGTCCGAAATATATAGGACTCTACCGGTCAGGGAAGAGATTTTAAAGGCACTTCTAAATGCGGACCTTATTGGTTTCCACACATTTGATTACGCTCGGCATTTCTTGTCTTGTTGTAGTCGGATGTTGGGTTTGGAGTATCAGTCGAAGCGGGGTTATATTGGGTTGGATTATTTCGGAAGGACAGTTGGGATAAAGATCATGCCTGTTGGGATTCACATGGGGCAGATTGAGTCAGTGTTGAAGCTTGCGGATAAGGAATGGAGGGTAGGAGAACTTAAGCAACAGTTTGAAGGAAAGACGGTGCTACTTGGTGTTGATGATATGGACATCTTTAAAGGTGTCAACTTGAAGCTCTTGGCAATGGAACAGATGCTGAAGCAGCATCCGAAGTGGCAGGGGAAAGCGGTTCTGGTTCAGATAGCGAACCCTGCTAGGGGAAGAGGGAAAGATCTTGAGGAAACACAGGCTGAAATACAGGCAAGCATCAAAAGAATTAATGAAAATTTTGGTCGGACTGGATATGAACCTATCGTGTTCATTGATAGACCAGTTTCTCTTAGTGAAAGAGTTGCATATTACACAATTGCAGAGTGTGTAGTAGTCACAGCTGTGAGAGATGGGATGAATCTTATTCCATACGAGTACATTGTGTGCAGGCAAGGAAACTCTCTATCAGATTCTAATTCAGAACACAGTGGGCCGAAGAAGAGCATGCTCGTAGTATCAGAGTTTATTGGATGTTCCCCTTCACTAAGTGGTGCAATTCGGGTTAACCCATGGAATATTGAATCAACGGCGGAGGCAATGAACGAGGCAATTTCAATGGTTGAACCCGAGAAGCAGTTGCGGCATGAGAAACATTATAGGTATGTTAGTACACATGATGTGGCATACTGGTCGCGAAGTGTTTTCCAAGATATGGAGAGAACTTGCAAAGAACATTTCAGAAGGCGTTGCTGGGGTATTGGTTTGGGCTTCGGCTTCAGGGTTATAGCACTTGATCCTAACTTCAGAAAGCTGACTATTGATCGCATCGTTTCTGCTTACTTGAGATCTAAAAATAGGGCAATTCTGTTGGATTATGATGGCACTGTGATGCCTCAAACATCTATAAATAAGAGCCCGAGTCAAGAGGTCATCTCCCTCATTAATACGCTTTGTGGTGATGTTAAAAACACTGTTTTTGTGGTCAGTGGAAGAGGAAGGGATAGTTTAAGTAAGTGGTTTTCTCCTTGCAAGAAACTAGGAATTGCTGCCGAACATGGTTATTTTGTGAGGTGGTCTGCTGACAAGGACTGGGAAGTTTGCGGGCAGAGCAATGACTTTGGTTGGATACAGATAGCTGAACCAGTTATGAAGCTCTACACAGAAGCCACCGATGGTTCCAGCATTGAAACCAAGGAGAGTGCCTTGGTTTGGCACCATCGAGATGCTGATCCTGGTTTTGGATCCAGCCAGGCTAAAGAGCTACTGGATCATTTAGAAAGTGTGCTAGCAAATGAACCAGTTGCTGTAAAGAGTGGTCAATACATTGTAGAAGTGAAGCCACAGGGTGTCAGTAAAGGCGTGGTTGCAGAAAAGATCTTCACATCAATGCATCAGACAGGGAAGCAGGCCGATTTTGTACTGTGTGTTGGCGACGATAGATCTGATGAGGATATGTTTGAGATCATTGGTAGTGCTAGGACAAATGGTGTCCTCTCTTCCAATACCAGTGTTTTCGCTTGCACTGTTGGACAGAAGCCCAGTAAAGCCAAGTACTATTTGGATGACCCTGGTGATGTTATTGCAATGCTTTACGCTCTTGCAGATGCTTCAGACTCTCCGCCTTCCTCGGATGGTGAACCAGGCTCTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0033554 GO:0034637 GO:0042578 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044424 GO:0044444 GO:0044464 GO:0046351 GO:0050896 GO:0051716 GO:0070413 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

855

Amino Acids

96.06

Weight (kDa)

6.01

Isoelectric Point (pI)

45.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_20	PF00982	60 - 543	2.7e-182	Glycosyltransferase family 20
Trehalose_PPase	PF02358	593 - 827	5.6e-80	Trehalose-phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002700)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06410 AT1G06410 AT1G06410
fragaria_vesca	FvH4_3g07260 FvH4_6g27760 FvH4_6g27760
malus_domestica	MD00G1008700.v1.1 MD09G1234800.v1.1 MD10G1275100.v1.1
prunus_persica	Prupe.3G113100_v2.0.a1 Prupe.3G113100_v2.0.a1 Prupe.3G113100_v2.0.a1 Prupe.3G113100_v2.0.a1 Prupe.3G113100_v2.0.a1 Prupe.4G067300_v2.0.a1
pyrus_communis	pycom05g27390 pycom09g15300 pycom10g22970
rosa_chinensis	RchiOBHm_Chr2g0131021 RchiOBHm_Chr5g0011801
rosa_laevigata	RLG00000019161 RLG00000031862
rosa_multiflora	Rmu_sc0003629.1_g000054 Rmu_ssc0000357.1_g000005
rosa_roxburghii	Rroxscaffold_1G00064760
rosa_rugosa	Rorug02G0294400 Rorug04G0459400
rosa_samantha	Rh2AG346700 Rh2BG354100 Rh2CG332700 Rh2DG372200 Rh5AG090700 Rh5BG086300 Rh5CG100100 Rh5DG086400
rosa_wichuraiana	Rw0G018970 Rw2G028110 Rw2G028150 Rw5G007920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2124
AccB7I	CCANNNNNTGG	2 cut(s)	628, 1624
AccI	GTMKAC	2 cut(s)	17, 292
AccII	CGCG	3 cut(s)	66, 85, 1606
AccIII	TCCGGA	2 cut(s)	41, 589
AclWI	GGATC	5 cut(s)	1700, 2135, 2148, 2161, 2187
AcoI	YGGCCR	1 cut(s)	543
AcsI	RAATTY	1 cut(s)	1207
AcuI	CTGAAG	4 cut(s)	1100, 1672, 1699, 2505
AdeI	CACNNNGTG	2 cut(s)	1460, 2258
AfaI	GTAC	4 cut(s)	1349, 1582, 2331, 2465
AfiI	CCNNNNNNNGG	8 cut(s)	59, 628, 648, 1138, 1139, 1624, 2121, 2150
AflII	CTTAAG	1 cut(s)	980
AgeI	ACCGGT	1 cut(s)	727
AhdI	GACNNNNNGTC	1 cut(s)	424
AjnI	CCWGG	4 cut(s)	2143, 2160, 2479, 2551
AleI	CACNNNNGTG	1 cut(s)	1313
Alw21I	GWGCWC	1 cut(s)	120
Alw26I	GTCTC	1 cut(s)	217
AlwI	GGATC	5 cut(s)	1700, 2135, 2148, 2161, 2187
AlwNI	CAGNNNCTG	3 cut(s)	1078, 2216, 2558
Ama87I	CYCGRG	2 cut(s)	1538, 1828
Aor13HI	TCCGGA	2 cut(s)	41, 589
AoxI	GGCC	5 cut(s)	402, 464, 543, 1403, 2319
ApeKI	GCWGC	4 cut(s)	454, 634, 1084, 1958
ApoI	RAATTY	1 cut(s)	1207
AseI	ATTAAT	2 cut(s)	1200, 1854
AsiGI	ACCGGT	1 cut(s)	727
AspS9I	GGNCC	3 cut(s)	175, 766, 1403
AsuHPI	GGTGA	5 cut(s)	583, 592, 1880, 2495, 2558
AsuNHI	GCTAGC	1 cut(s)	2197
AvaI	CYCGRG	2 cut(s)	1538, 1828
AvaII	GGWCC	2 cut(s)	175, 766
BamHI	GGATCC	1 cut(s)	2153
BanI	GGYRCC	1 cut(s)	2124
BanII	GRGCYC	2 cut(s)	120, 1829
Bbv12I	GWGCWC	1 cut(s)	120
BbvI	GCAGC	4 cut(s)	466, 621, 1096, 1945
BccI	CCATC	7 cut(s)	234, 630, 1316, 1784, 2078, 2136, 2537
BceAI	ACGGC	2 cut(s)	89, 1515
BcgI	CGANNNNNNTGC	2 cut(s)	1584, 1618
BciT130I	CCWGG	4 cut(s)	2145, 2162, 2481, 2553
BciVI	GTATCC	1 cut(s)	2031
BclI	TGATCA	1 cut(s)	127
BcoDI	GTCTC	1 cut(s)	217
BfaI	CTAG	4 cut(s)	1137, 1949, 2198, 2388
BfrI	CTTAAG	1 cut(s)	980
BfuI	GTATCC	1 cut(s)	2031
BglII	AGATCT	5 cut(s)	31, 1153, 1757, 2286, 2351
BisI	GCNGC	8 cut(s)	77, 413, 455, 546, 635, 1085, 1553, 1959
BlpI	GCTNAGC	1 cut(s)	216
BlsI	GCNGC	8 cut(s)	78, 414, 456, 547, 636, 1086, 1554, 1960
BmcAI	AGTACT	1 cut(s)	2465
Bme1390I	CCNGG	4 cut(s)	2145, 2162, 2481, 2553
Bme18I	GGWCC	2 cut(s)	175, 766
BmeRI	GACNNNNNGTC	1 cut(s)	424
BmeT110I	CYCGRG	2 cut(s)	1538, 1828
BmgT120I	GGNCC	3 cut(s)	175, 766, 1403
BmiI	GGNNCC	3 cut(s)	2089, 2126, 2155
BmrFI	CCNGG	4 cut(s)	2145, 2162, 2481, 2553
BmrI	ACTGGG	2 cut(s)	2011, 2445
BmsI	GCATC	8 cut(s)	1065, 1096, 1197, 1746, 1791, 2125, 2311, 2506
BmtI	GCTAGC	1 cut(s)	2201
BmuI	ACTGGG	2 cut(s)	2011, 2445
BplI	GAGNNNNNCTC	2 cut(s)	1829, 1861
Bpu1102I	GCTNAGC	1 cut(s)	216
BpuEI	CTTGAG	3 cut(s)	667, 1178, 1774
BsaBI	GATNNNNATC	4 cut(s)	126, 621, 899, 2139
BsaI	GGTCTC	1 cut(s)	217
BsaJI	CCNNGG	5 cut(s)	1481, 2103, 2115, 2479, 2538
BsaWI	WCCGGW	7 cut(s)	41, 52, 177, 226, 295, 589, 727
BsaXI	ACNNNNNCTCC	6 cut(s)	235, 265, 299, 329, 830, 860
Bsc4I	CCNNNNNNNGG	8 cut(s)	59, 628, 648, 1138, 1139, 1624, 2121, 2150
Bse118I	RCCGGY	1 cut(s)	727
Bse1I	ACTGG	9 cut(s)	1225, 1253, 1604, 2006, 2054, 2181, 2210, 2418, 2451
Bse3DI	GCAATG	4 cut(s)	1071, 1515, 2029, 2502
Bse8I	GATNNNNATC	4 cut(s)	126, 621, 899, 2139
BseAI	TCCGGA	2 cut(s)	41, 589
BseBI	CCWGG	4 cut(s)	2145, 2162, 2481, 2553
BseDI	CCNNGG	5 cut(s)	1481, 2103, 2115, 2479, 2538
BseJI	GATNNNNATC	4 cut(s)	126, 621, 899, 2139
BseLI	CCNNNNNNNGG	8 cut(s)	59, 628, 648, 1138, 1139, 1624, 2121, 2150
BseMI	GCAATG	4 cut(s)	1071, 1515, 2029, 2502
BseMII	CTCAG	1 cut(s)	507
BseNI	ACTGG	9 cut(s)	1225, 1253, 1604, 2006, 2054, 2181, 2210, 2418, 2451
BseRI	GAGGAG	1 cut(s)	664
BseXI	GCAGC	4 cut(s)	466, 621, 1096, 1945
BseYI	CCCAGC	1 cut(s)	1662
BsgI	GTGCAG	1 cut(s)	1378
Bsh1236I	CGCG	3 cut(s)	66, 85, 1606
Bsh1285I	CGRYCG	1 cut(s)	520
BshFI	GGCC	5 cut(s)	404, 466, 545, 1405, 2321
BshNI	GGYRCC	1 cut(s)	2124
BshTI	ACCGGT	1 cut(s)	727
BsiEI	CGRYCG	1 cut(s)	520
BsiHKAI	GWGCWC	1 cut(s)	120
BsiHKCI	CYCGRG	2 cut(s)	1538, 1828
BsiSI	CCGG	8 cut(s)	42, 53, 178, 227, 296, 401, 590, 728
BslI	CCNNNNNNNGG	8 cut(s)	59, 628, 648, 1138, 1139, 1624, 2121, 2150
BsmAI	GTCTC	1 cut(s)	217
BsnI	GGCC	5 cut(s)	404, 466, 545, 1405, 2321
Bso31I	GGTCTC	1 cut(s)	217
BsoBI	CYCGRG	2 cut(s)	1538, 1828
Bsp1286I	GDGCHC	2 cut(s)	120, 1829
Bsp13I	TCCGGA	2 cut(s)	41, 589
Bsp1720I	GCTNAGC	1 cut(s)	216
Bsp19I	CCATGG	1 cut(s)	1481
Bsp68I	TCGCGA	2 cut(s)	66, 1606
BspANI	GGCC	5 cut(s)	404, 466, 545, 1405, 2321
BspCNI	CTCAG	1 cut(s)	506
BspEI	TCCGGA	2 cut(s)	41, 589
BspFNI	CGCG	3 cut(s)	66, 85, 1606
BspHI	TCATGA	1 cut(s)	613
BspLI	GGNNCC	3 cut(s)	2089, 2126, 2155
BspOI	GCTAGC	1 cut(s)	2201
BspPI	GGATC	5 cut(s)	1700, 2135, 2148, 2161, 2187
BspQI	GCTCTTC	1 cut(s)	1406
BspT107I	GGYRCC	1 cut(s)	2124
BspTI	CTTAAG	1 cut(s)	980
BspTNI	GGTCTC	1 cut(s)	217
BsrDI	GCAATG	4 cut(s)	1071, 1515, 2029, 2502
BsrFI	RCCGGY	1 cut(s)	727
BsrI	ACTGG	9 cut(s)	1225, 1253, 1604, 2006, 2054, 2181, 2210, 2418, 2451
BssAI	RCCGGY	1 cut(s)	727
BssECI	CCNNGG	5 cut(s)	1481, 2103, 2115, 2479, 2538
BssNAI	GTATAC	1 cut(s)	18
BssT1I	CCWWGG	3 cut(s)	1481, 2103, 2115
Bst1107I	GTATAC	1 cut(s)	18
Bst2UI	CCWGG	4 cut(s)	2145, 2162, 2481, 2553
Bst6I	CTCTTC	5 cut(s)	732, 1138, 1406, 1897, 2413
BstAFI	CTTAAG	1 cut(s)	980
BstC8I	GCNNGC	9 cut(s)	452, 951, 1186, 1361, 1418, 2015, 2199, 2319, 2431
BstDEI	CTNAG	4 cut(s)	216, 493, 1262, 1457
BstDSI	CCRYGG	1 cut(s)	1481
BstENI	CCTNNNNNAGG	1 cut(s)	646
BstFNI	CGCG	3 cut(s)	66, 85, 1606
BstMAI	GTCTC	1 cut(s)	217
BstMCI	CGRYCG	1 cut(s)	520
BstMWI	GCNNNNNNNGC	7 cut(s)	82, 463, 542, 1084, 1133, 1552, 2019
BstNI	CCWGG	4 cut(s)	2145, 2162, 2481, 2553
BstNSI	RCATGY	2 cut(s)	484, 1420
BstSCI	CCNGG	4 cut(s)	2143, 2160, 2479, 2551
BstUI	CGCG	3 cut(s)	66, 85, 1606
BstV1I	GCAGC	4 cut(s)	466, 621, 1096, 1945
BstX2I	RGATCY	6 cut(s)	31, 1153, 1757, 2153, 2286, 2351
BstYI	RGATCY	6 cut(s)	31, 1153, 1757, 2153, 2286, 2351
BstZ17I	GTATAC	1 cut(s)	18
BsuI	GTATCC	1 cut(s)	2031
BsuRI	GGCC	5 cut(s)	404, 466, 545, 1405, 2321
BtgI	CCRYGG	1 cut(s)	1481
BtsIMutI	CAGTG	7 cut(s)	945, 1405, 1794, 1881, 1903, 2425, 2433
BtuMI	TCGCGA	2 cut(s)	66, 1606
Cac8I	GCNNGC	9 cut(s)	452, 951, 1186, 1361, 1418, 2015, 2199, 2319, 2431
CaiI	CAGNNNCTG	3 cut(s)	1078, 2216, 2558
CciI	TCATGA	1 cut(s)	613
Cfr10I	RCCGGY	1 cut(s)	727
Cfr13I	GGNCC	3 cut(s)	175, 766, 1403
CseI	GACGC	1 cut(s)	322
Csp6I	GTAC	4 cut(s)	1348, 1581, 2330, 2464
CspAI	ACCGGT	1 cut(s)	727
CviQI	GTAC	4 cut(s)	1348, 1581, 2330, 2464
DdeI	CTNAG	4 cut(s)	216, 493, 1262, 1457
DraI	TTTAAA	2 cut(s)	747, 1039
DraIII	CACNNNGTG	2 cut(s)	1460, 2258
DriI	GACNNNNNGTC	1 cut(s)	424
EaeI	YGGCCR	1 cut(s)	543
Eam1104I	CTCTTC	5 cut(s)	732, 1138, 1406, 1897, 2413
Eam1105I	GACNNNNNGTC	1 cut(s)	424
EarI	CTCTTC	5 cut(s)	732, 1138, 1406, 1897, 2413
EciI	GGCGGA	2 cut(s)	1517, 2520
Ecl136II	GAGCTC	1 cut(s)	118
Eco130I	CCWWGG	3 cut(s)	1481, 2103, 2115
Eco24I	GRGCYC	2 cut(s)	120, 1829
Eco31I	GGTCTC	1 cut(s)	217
Eco47I	GGWCC	2 cut(s)	175, 766
Eco53kI	GAGCTC	1 cut(s)	118
Eco57I	CTGAAG	4 cut(s)	1100, 1672, 1699, 2505
Eco88I	CYCGRG	2 cut(s)	1538, 1828
EcoICRI	GAGCTC	1 cut(s)	118
EcoNI	CCTNNNNNAGG	1 cut(s)	646
EcoRII	CCWGG	4 cut(s)	2143, 2160, 2479, 2551
EcoT14I	CCWWGG	3 cut(s)	1481, 2103, 2115
EcoT22I	ATGCAT	1 cut(s)	2304
EcoT38I	GRGCYC	2 cut(s)	120, 1829
ErhI	CCWWGG	3 cut(s)	1481, 2103, 2115
FauI	CCCGC	3 cut(s)	127, 846, 2006
FbaI	TGATCA	1 cut(s)	127
FblI	GTMKAC	2 cut(s)	17, 292
Fnu4HI	GCNGC	8 cut(s)	77, 413, 455, 546, 635, 1085, 1553, 1959
FriOI	GRGCYC	2 cut(s)	120, 1829
Fsp4HI	GCNGC	8 cut(s)	77, 413, 455, 546, 635, 1085, 1553, 1959
FspBI	CTAG	4 cut(s)	1137, 1949, 2198, 2388
GluI	GCNGC	8 cut(s)	77, 413, 455, 546, 635, 1085, 1553, 1959
GsaI	CCCAGC	1 cut(s)	1666
HaeIII	GGCC	5 cut(s)	404, 466, 545, 1405, 2321
HapII	CCGG	8 cut(s)	42, 53, 178, 227, 296, 401, 590, 728
HgaI	GACGC	1 cut(s)	322
HincII	GTYRAC	2 cut(s)	1048, 1477
HindII	GTYRAC	2 cut(s)	1048, 1477
HindIII	AAGCTT	1 cut(s)	947
HpaI	GTTAAC	1 cut(s)	1477
HpaII	CCGG	8 cut(s)	42, 53, 178, 227, 296, 401, 590, 728
HphI	GGTGA	5 cut(s)	583, 592, 1880, 2495, 2558
Hpy166II	GTNNAC	6 cut(s)	18, 293, 1048, 1477, 1583, 2549
Hpy8I	GTNNAC	6 cut(s)	18, 293, 1048, 1477, 1583, 2549
Hpy99I	CGWCG	6 cut(s)	101, 151, 163, 166, 338, 2348
HpyAV	CCTTC	9 cut(s)	277, 652, 711, 876, 989, 1461, 1647, 1902, 2544
HpyF10VI	GCNNNNNNNGC	7 cut(s)	82, 463, 542, 1084, 1133, 1552, 2019
HpyF3I	CTNAG	4 cut(s)	216, 493, 1262, 1457
Kpn2I	TCCGGA	2 cut(s)	41, 589
Ksp22I	TGATCA	1 cut(s)	127
KspAI	GTTAAC	1 cut(s)	1477
LguI	GCTCTTC	1 cut(s)	1406
LmnI	GCTCC	3 cut(s)	115, 207, 243
Lsp1109I	GCAGC	4 cut(s)	466, 621, 1096, 1945
LweI	GCATC	8 cut(s)	1065, 1096, 1197, 1746, 1791, 2125, 2311, 2506
MaeI	CTAG	4 cut(s)	1137, 1949, 2198, 2388
MaeIII	GTNAC	1 cut(s)	1306
MboII	GAAGA	8 cut(s)	311, 749, 1155, 1420, 1423, 1914, 2281, 2400
MfeI	CAATTG	1 cut(s)	1287
MflI	RGATCY	6 cut(s)	31, 1153, 1757, 2153, 2286, 2351
MhlI	GDGCHC	2 cut(s)	120, 1829
MluCI	AATT	9 cut(s)	183, 1197, 1207, 1287, 1387, 1467, 1521, 1773, 1953
MlyI	GAGTC	5 cut(s)	257, 715, 944, 1840, 2519
MmeI	TCCRAC	8 cut(s)	129, 360, 803, 849, 1196, 1761, 2015, 2419
Mph1103I	ATGCAT	1 cut(s)	2304
MroI	TCCGGA	2 cut(s)	41, 589
MseI	TTAA	8 cut(s)	746, 981, 1038, 1200, 1476, 1854, 1875, 1919
MslI	CAYNNNNRTG	4 cut(s)	1313, 1799, 2133, 2297
MspA1I	CMGCKG	1 cut(s)	1313
MspCI	CTTAAG	1 cut(s)	980
MspI	CCGG	8 cut(s)	42, 53, 178, 227, 296, 401, 590, 728
MspR9I	CCNGG	4 cut(s)	2145, 2162, 2481, 2553
MunI	CAATTG	1 cut(s)	1287
MvaI	CCWGG	4 cut(s)	2145, 2162, 2481, 2553
MvnI	CGCG	3 cut(s)	66, 85, 1606
MwoI	GCNNNNNNNGC	7 cut(s)	82, 463, 542, 1084, 1133, 1552, 2019
NcoI	CCATGG	1 cut(s)	1481
NheI	GCTAGC	1 cut(s)	2197
NlaIV	GGNNCC	3 cut(s)	2089, 2126, 2155
NmeAIII	GCCGAG	1 cut(s)	778
NmuCI	GTSAC	1 cut(s)	1306
NruI	TCGCGA	2 cut(s)	66, 1606
NsiI	ATGCAT	1 cut(s)	2304
NspI	RCATGY	2 cut(s)	484, 1420
OliI	CACNNNNGTG	1 cut(s)	1313
PaeI	GCATGC	1 cut(s)	1420
PagI	TCATGA	1 cut(s)	613
PciSI	GCTCTTC	1 cut(s)	1406
PcsI	WCGNNNNNNNCGW	1 cut(s)	155
PfeI	GAWTC	8 cut(s)	196, 344, 387, 610, 916, 1330, 1382, 1493
PflMI	CCANNNNNTGG	2 cut(s)	628, 1624
PinAI	ACCGGT	1 cut(s)	727
PkrI	GCNGC	8 cut(s)	78, 414, 456, 547, 636, 1086, 1554, 1960
Ple19I	CGATCG	1 cut(s)	520
PleI	GAGTC	5 cut(s)	257, 715, 943, 1839, 2519
PpsI	GAGTC	5 cut(s)	257, 715, 943, 1839, 2519
PshBI	ATTAAT	2 cut(s)	1200, 1854
Psp124BI	GAGCTC	1 cut(s)	120
Psp6I	CCWGG	4 cut(s)	2143, 2160, 2479, 2551
PspFI	CCCAGC	1 cut(s)	1662
PspGI	CCWGG	4 cut(s)	2143, 2160, 2479, 2551
PspN4I	GGNNCC	3 cut(s)	2089, 2126, 2155
PspPI	GGNCC	3 cut(s)	175, 766, 1403
PstNI	CAGNNNCTG	3 cut(s)	1078, 2216, 2558
PsuI	RGATCY	6 cut(s)	31, 1153, 1757, 2153, 2286, 2351
PvuI	CGATCG	1 cut(s)	520
PvuII	CAGCTG	1 cut(s)	1313
RruI	TCGCGA	2 cut(s)	66, 1606
RsaI	GTAC	4 cut(s)	1349, 1582, 2331, 2465
RsaNI	GTAC	4 cut(s)	1348, 1581, 2330, 2464
RseI	CAYNNNNRTG	4 cut(s)	1313, 1799, 2133, 2297
SacI	GAGCTC	1 cut(s)	120
SapI	GCTCTTC	1 cut(s)	1406
SaqAI	TTAA	8 cut(s)	746, 981, 1038, 1200, 1476, 1854, 1875, 1919
SatI	GCNGC	8 cut(s)	77, 413, 455, 546, 635, 1085, 1553, 1959
Sau96I	GGNCC	3 cut(s)	175, 766, 1403
ScaI	AGTACT	1 cut(s)	2465
SchI	GAGTC	5 cut(s)	257, 715, 944, 1840, 2519
ScrFI	CCNGG	4 cut(s)	2145, 2162, 2481, 2553
SduI	GDGCHC	2 cut(s)	120, 1829
SfaNI	GCATC	8 cut(s)	1065, 1096, 1197, 1746, 1791, 2125, 2311, 2506
SinI	GGWCC	2 cut(s)	175, 766
SmiMI	CAYNNNNRTG	4 cut(s)	1313, 1799, 2133, 2297
SmlI	CTYRAG	4 cut(s)	646, 980, 1157, 1753
SmoI	CTYRAG	4 cut(s)	646, 980, 1157, 1753
SphI	GCATGC	1 cut(s)	1420
Sse9I	AATT	9 cut(s)	183, 1197, 1207, 1287, 1387, 1467, 1521, 1773, 1953
SspI	AATATT	1 cut(s)	1489
SspMI	CTAG	4 cut(s)	1137, 1949, 2198, 2388
SstI	GAGCTC	1 cut(s)	120
StyD4I	CCNGG	4 cut(s)	2143, 2160, 2479, 2551
StyI	CCWWGG	3 cut(s)	1481, 2103, 2115
TaqI	TCGA	7 cut(s)	120, 164, 336, 516, 576, 848, 2131
TaqII	GACCGA	1 cut(s)	510
TasI	AATT	9 cut(s)	183, 1197, 1207, 1287, 1387, 1467, 1521, 1773, 1953
TatI	WGTACW	4 cut(s)	1347, 1580, 2329, 2463
TauI	GCSGC	4 cut(s)	79, 415, 548, 1555
TfiI	GAWTC	8 cut(s)	196, 344, 387, 610, 916, 1330, 1382, 1493
Tru1I	TTAA	8 cut(s)	746, 981, 1038, 1200, 1476, 1854, 1875, 1919
Tru9I	TTAA	8 cut(s)	746, 981, 1038, 1200, 1476, 1854, 1875, 1919
TscAI	CASTG	7 cut(s)	945, 1405, 1801, 1888, 1903, 2425, 2440
TseFI	GTSAC	1 cut(s)	1306
TseI	GCWGC	4 cut(s)	454, 634, 1084, 1958
Tsp45I	GTSAC	1 cut(s)	1306
TspGWI	ACGGA	1 cut(s)	147
TspRI	CASTG	7 cut(s)	945, 1405, 1801, 1888, 1903, 2425, 2440
Van91I	CCANNNNNTGG	2 cut(s)	628, 1624
Vha464I	CTTAAG	1 cut(s)	980
VpaK11BI	GGWCC	2 cut(s)	175, 766
VspI	ATTAAT	2 cut(s)	1200, 1854
XagI	CCTNNNNNAGG	1 cut(s)	646
XapI	RAATTY	1 cut(s)	1207
XceI	RCATGY	2 cut(s)	484, 1420
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2467
XmiI	GTMKAC	2 cut(s)	17, 292
XspI	CTAG	4 cut(s)	1137, 1949, 2198, 2388
ZrmI	AGTACT	1 cut(s)	2465
Zsp2I	ATGCAT	1 cut(s)	2304

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.