MD09G1253500.v1.1

DUF761-associated sequence motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011441
Physical Location & Seq
Forward (+)
32468100 .. 32470229
2130 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1253500.v1.1.491

Sequence Viewer

Length: 1551 bp
ATGCCGATTTCCAAGCTACATTTGATTGCTGATGAAAACAGTGGGGGTTTCCTAAATGCGAAGAAAAACGGGAATCCTAGTGTTGATTATGAGCACAAGCATGAACTGCGTGCTCCGAGTTTGGTTGCTAGGCTTATGGGTCTTGAATCCATGCCTGCCACACGCGAGCAGCCCAAGAAAGCTTCGTTTGCTGATGTTAGTGACAGTGGAGAGAAGACAATTGTGAATAACTGTAGTGGTAATGATAGAGAAGAACTGAATTTGGAGACACAAAATGGAAAGTCTGAAGCAAGGCCTCAGAAGCTGCAGAAAATGGGACCGTATGAGAAGAGGGCAGTGACTAGGTTTGGAGCCGAGGCACTTCAAATTAAGAGTGTTTTGTCGTGCTCGAGAAAGCATCACCCAAAGCTTGCTTCTCCAGTGAAGAGTCCTAGGATTTCCTCTGGGAAGAATGCTTCGCGAAACTCTCGGTTGATTGGTGCTGCTACTAGAATTTTAGAACCTGGACTGCAGTCTACGAATAGAGCAAAAGGTGCCCTCACTTACTCACCTTCGTTTCAATATCCTCCGGTGTGTGAGGTTGTGGAAGATGGAGCAACAGTTCAATCACCAGAGATTTCTAAGCATGCACGTTGTAATGCTGGTGCGTCCAATTCTCTGATAGGCCAAACTTCTTGCAAAAGCTGTGGTAGTTCAGTAGATGTTGTGGAATCATCGTCAAAGGTGGAGGCACAACAACCTGCTTTCCCATCCTTTGCTTCAAACATTATCAATGGCTCTTCGATGGTAGCAGAACAAAGTAAGCCGAAGTCATCCATATCCTCCTTTGGGAAAGAAAAGGATGCAATTTCTCAGGGAACTCGGAACCAACCTGTATCAGTTTCTGGTCAAAAGGGAATACGATCCCTTGGTGAACCTGTCACAGAAGGGAAGTCTCTACCACCAGAAGGTCTAGCTTCATGGCAGTTGTTGTCAAATCAACCATGCAAACCTCAGAAGGGAGAAGCATCTTCGATTACCTCAAAGAATAGGTCTCAGATGCAGCATCGAATGTCACTAGGAAGAGAAAGGATTCCACCCAGATCCAAACTAAATAGTCTTGATGGAAGAAGAGACTCATCTGCTGCCAATGCTGTTACAGGGACCAAAGACTTTGTTGCCTTGAACCGAAATCTGAGTGTTCGTTCCCGACCAAGGGTGCTTACCAAAATGAACGATTATAAGTTTGACACAGAGAAGGAAGCCTTTAATGGAAAAGATGATTATCCATCACAGCTAAGGACCACGATACGGAAAAGGAGGATTATCAATGGTAGTGGCCAAGTTGAAAGTAGTGGTTTTGTTGGTTCAACTTCCACAAGACGGGGGAATTATCAGTTTGATGTGCCAACTGGAAAGAGATTAGGAAATGGTGTTCATTTTATGAACCACACTTCTGCTAGGAATAAATTAGCAGGTCAGAGAGAAGGTAACAGAGCAAATGGAAATAAGGGCACTGATGTTATTTCATTCACATTTAATTCCCCTATCAGGAACAGGACTGGACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 VARLMGL

Protein Analysis

517

Amino Acids

55.88

Weight (kDa)

10.04

Isoelectric Point (pI)

47.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VARLMGL PF14383 28 - 54 1.6e-11 DUF761-associated sequence motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1221
Acc36I ACCTGC 2 cut(s) 748, 1445
AccB1I GGYRCC 1 cut(s) 533
AccI GTMKAC 1 cut(s) 515
AccII CGCG 2 cut(s) 165, 460
AclWI GGATC 2 cut(s) 897, 1077
AcoI YGGCCR 1 cut(s) 1318
AcsI RAATTY 2 cut(s) 259, 492
AcuI CTGAAG 1 cut(s) 306
AfiI CCNNNNNNNGG 8 cut(s) 828, 947, 998, 1194, 1195, 1290, 1362, 1530
AgsI TTSAA 8 cut(s) 146, 365, 560, 605, 762, 1165, 1328, 1350
AjnI CCWGG 1 cut(s) 502
AjuI GAANNNNNNNTTGG 2 cut(s) 397, 429
AloI GAACNNNNNNTCC 4 cut(s) 585, 617, 1398, 1430
AluBI AGCT 7 cut(s) 16, 182, 304, 409, 684, 956, 1276
AluI AGCT 7 cut(s) 16, 182, 304, 409, 684, 956, 1276
Alw21I GWGCWC 3 cut(s) 96, 115, 389
Alw26I GTCTC 4 cut(s) 260, 939, 1038, 1107
AlwI GGATC 2 cut(s) 897, 1077
AlwNI CAGNNNCTG 2 cut(s) 304, 884
Ama87I CYCGRG 1 cut(s) 388
AoxI GGCC 3 cut(s) 293, 664, 1318
ApeKI GCWGC 5 cut(s) 169, 304, 482, 1042, 1124
ApoI RAATTY 2 cut(s) 259, 492
ArsI GACNNNNNNTTYG 2 cut(s) 1016, 1048
Asp700I GAANNNNTTC 1 cut(s) 1071
AspA2I CCTAGG 1 cut(s) 431
AspS9I GGNCC 4 cut(s) 317, 1143, 1281, 1544
AsuHPI GGTGA 4 cut(s) 392, 540, 600, 923
AvaI CYCGRG 1 cut(s) 388
AvaII GGWCC 4 cut(s) 317, 1143, 1281, 1544
AvrII CCTAGG 1 cut(s) 431
BaeGI GKGCMC 2 cut(s) 538, 1496
BalI TGGCCA 1 cut(s) 1320
BanI GGYRCC 1 cut(s) 533
BbsI GAAGAC 1 cut(s) 221
Bbv12I GWGCWC 3 cut(s) 96, 115, 389
BbvI GCAGC 5 cut(s) 181, 291, 469, 1054, 1111
BccI CCATC 5 cut(s) 584, 757, 778, 1097, 1276
BciT130I CCWGG 1 cut(s) 504
BcoDI GTCTC 4 cut(s) 260, 939, 1038, 1107
BfaI CTAG 8 cut(s) 78, 129, 342, 432, 489, 953, 1058, 1440
BfmI CTRYAG 3 cut(s) 232, 305, 509
BfuAI ACCTGC 2 cut(s) 748, 1445
BisI GCNGC 5 cut(s) 170, 305, 483, 1043, 1125
BlnI CCTAGG 1 cut(s) 431
BlsI GCNGC 5 cut(s) 171, 306, 484, 1044, 1126
Bme1390I CCNGG 1 cut(s) 504
Bme18I GGWCC 4 cut(s) 317, 1143, 1281, 1544
BmeT110I CYCGRG 1 cut(s) 388
BmgT120I GGNCC 4 cut(s) 317, 1143, 1281, 1544
BmiI GGNNCC 6 cut(s) 318, 352, 535, 866, 1144, 1546
BmrFI CCNGG 1 cut(s) 504
BmsI GCATC 5 cut(s) 406, 832, 1016, 1029, 1054
BoxI GACNNNNGTC 1 cut(s) 511
BpiI GAAGAC 1 cut(s) 221
BpmI CTGGAG 1 cut(s) 402
Bpu10I CCTNAGC 1 cut(s) 1277
BsaBI GATNNNNATC 1 cut(s) 1263
BsaI GGTCTC 1 cut(s) 1038
BsaJI CCNNGG 4 cut(s) 354, 431, 907, 1193
BsaWI WCCGGW 1 cut(s) 568
BsaXI ACNNNNNCTCC 2 cut(s) 585, 615
Bsc4I CCNNNNNNNGG 8 cut(s) 828, 947, 998, 1194, 1195, 1290, 1362, 1530
Bse1I ACTGG 3 cut(s) 419, 1396, 1546
Bse8I GATNNNNATC 1 cut(s) 1263
BseBI CCWGG 1 cut(s) 504
BseDI CCNNGG 4 cut(s) 354, 431, 907, 1193
BseGI GGATG 3 cut(s) 749, 812, 847
BseJI GATNNNNATC 1 cut(s) 1263
BseLI CCNNNNNNNGG 8 cut(s) 828, 947, 998, 1194, 1195, 1290, 1362, 1530
BseMII CTCAG 5 cut(s) 311, 866, 1007, 1049, 1166
BseNI ACTGG 3 cut(s) 419, 1396, 1546
BseSI GKGCMC 2 cut(s) 538, 1496
BseXI GCAGC 5 cut(s) 181, 291, 469, 1054, 1111
Bsh1236I CGCG 2 cut(s) 165, 460
BshFI GGCC 3 cut(s) 295, 666, 1320
BshNI GGYRCC 1 cut(s) 533
BsiHKAI GWGCWC 3 cut(s) 96, 115, 389
BsiHKCI CYCGRG 1 cut(s) 388
BsiSI CCGG 1 cut(s) 569
BslFI GGGAC 2 cut(s) 330, 1156
BslI CCNNNNNNNGG 8 cut(s) 828, 947, 998, 1194, 1195, 1290, 1362, 1530
BsmAI GTCTC 4 cut(s) 260, 939, 1038, 1107
BsmFI GGGAC 2 cut(s) 330, 1156
BsmI GAATGC 1 cut(s) 457
BsnI GGCC 3 cut(s) 295, 666, 1320
Bso31I GGTCTC 1 cut(s) 1038
BsoBI CYCGRG 1 cut(s) 388
Bsp1286I GDGCHC 5 cut(s) 96, 115, 389, 538, 1496
Bsp143I GATC 2 cut(s) 902, 1082
Bsp68I TCGCGA 1 cut(s) 460
BspANI GGCC 3 cut(s) 295, 666, 1320
BspCNI CTCAG 5 cut(s) 310, 865, 1006, 1048, 1167
BspFNI CGCG 2 cut(s) 165, 460
BspLI GGNNCC 6 cut(s) 318, 352, 535, 866, 1144, 1546
BspMAI CTGCAG 2 cut(s) 309, 513
BspMI ACCTGC 2 cut(s) 748, 1445
BspPI GGATC 2 cut(s) 897, 1077
BspQI GCTCTTC 1 cut(s) 784
BspT107I GGYRCC 1 cut(s) 533
BspTNI GGTCTC 1 cut(s) 1038
BsrI ACTGG 3 cut(s) 419, 1396, 1546
BssECI CCNNGG 4 cut(s) 354, 431, 907, 1193
BssMI GATC 2 cut(s) 902, 1082
BssT1I CCWWGG 3 cut(s) 431, 907, 1193
Bst2UI CCWGG 1 cut(s) 504
Bst4CI ACNGT 5 cut(s) 41, 206, 233, 321, 601
Bst6I CTCTTC 5 cut(s) 323, 419, 784, 1057, 1105
BstAPI GCANNNNNTGC 2 cut(s) 106, 533
BstC8I GCNNGC 5 cut(s) 111, 156, 167, 411, 627
BstDEI CTNAG 7 cut(s) 297, 621, 852, 993, 1035, 1175, 1277
BstF5I GGATG 3 cut(s) 749, 812, 847
BstFNI CGCG 2 cut(s) 165, 460
BstKTI GATC 2 cut(s) 905, 1085
BstMAI GTCTC 4 cut(s) 260, 939, 1038, 1107
BstMBI GATC 2 cut(s) 902, 1082
BstMWI GCNNNNNNNGC 5 cut(s) 106, 188, 301, 533, 1130
BstNI CCWGG 1 cut(s) 504
BstNSI RCATGY 1 cut(s) 629
BstPAI GACNNNNGTC 1 cut(s) 511
BstSCI CCNGG 1 cut(s) 502
BstSFI CTRYAG 3 cut(s) 232, 305, 509
BstSLI GKGCMC 2 cut(s) 538, 1496
BstUI CGCG 2 cut(s) 165, 460
BstV1I GCAGC 5 cut(s) 181, 291, 469, 1054, 1111
BstV2I GAAGAC 1 cut(s) 221
BstX2I RGATCY 1 cut(s) 1082
BstYI RGATCY 1 cut(s) 1082
BsuRI GGCC 3 cut(s) 295, 666, 1320
BtsCI GGATG 3 cut(s) 749, 812, 847
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 5 cut(s) 46, 211, 342, 426, 1494
BtuMI TCGCGA 1 cut(s) 460
BveI ACCTGC 2 cut(s) 748, 1445
Cac8I GCNNGC 5 cut(s) 111, 156, 167, 411, 627
CaiI CAGNNNCTG 2 cut(s) 304, 884
Cfr13I GGNCC 4 cut(s) 317, 1143, 1281, 1544
CseI GACGC 1 cut(s) 636
CspCI CAANNNNNGTGG 4 cut(s) 667, 702, 1297, 1332
CviAII CATG 5 cut(s) 101, 151, 626, 960, 984
DdeI CTNAG 7 cut(s) 297, 621, 852, 993, 1035, 1175, 1277
DpnI GATC 2 cut(s) 904, 1084
DpnII GATC 2 cut(s) 902, 1082
EaeI YGGCCR 1 cut(s) 1318
Eam1104I CTCTTC 5 cut(s) 323, 419, 784, 1057, 1105
EarI CTCTTC 5 cut(s) 323, 419, 784, 1057, 1105
Eco130I CCWWGG 3 cut(s) 431, 907, 1193
Eco147I AGGCCT 1 cut(s) 295
Eco31I GGTCTC 1 cut(s) 1038
Eco47I GGWCC 4 cut(s) 317, 1143, 1281, 1544
Eco57I CTGAAG 1 cut(s) 306
Eco88I CYCGRG 1 cut(s) 388
EcoRII CCWGG 1 cut(s) 502
EcoT14I CCWWGG 3 cut(s) 431, 907, 1193
ErhI CCWWGG 3 cut(s) 431, 907, 1193
FaeI CATG 5 cut(s) 104, 154, 629, 963, 987
FalI AAGNNNNNCTT 2 cut(s) 1229, 1261
FaqI GGGAC 2 cut(s) 330, 1156
FatI CATG 5 cut(s) 100, 150, 625, 959, 983
FblI GTMKAC 1 cut(s) 515
Fnu4HI GCNGC 5 cut(s) 170, 305, 483, 1043, 1125
FokI GGATG 3 cut(s) 736, 799, 854
Fsp4HI GCNGC 5 cut(s) 170, 305, 483, 1043, 1125
FspBI CTAG 8 cut(s) 78, 129, 342, 432, 489, 953, 1058, 1440
GluI GCNGC 5 cut(s) 170, 305, 483, 1043, 1125
GsuI CTGGAG 1 cut(s) 402
HaeIII GGCC 3 cut(s) 295, 666, 1320
HapII CCGG 1 cut(s) 569
HgaI GACGC 1 cut(s) 636
Hin1II CATG 5 cut(s) 104, 154, 629, 963, 987
HindIII AAGCTT 2 cut(s) 180, 407
HinfI GANTC 6 cut(s) 73, 146, 427, 710, 1072, 1115
HpaII CCGG 1 cut(s) 569
HphI GGTGA 4 cut(s) 392, 540, 600, 923
Hpy166II GTNNAC 2 cut(s) 516, 914
Hpy188I TCNGA 9 cut(s) 117, 286, 300, 660, 864, 996, 1038, 1176, 1461
Hpy188III TCNNGA 6 cut(s) 143, 390, 459, 1100, 1188, 1531
Hpy8I GTNNAC 2 cut(s) 516, 914
HpyAV CCTTC 6 cut(s) 561, 920, 941, 991, 1231, 1460
HpyCH4III ACNGT 5 cut(s) 41, 206, 233, 321, 601
HpyCH4IV ACGT 1 cut(s) 631
HpyCH4V TGCA 7 cut(s) 307, 511, 629, 678, 845, 987, 1042
HpyF10VI GCNNNNNNNGC 5 cut(s) 106, 188, 301, 533, 1130
HpyF3I CTNAG 7 cut(s) 297, 621, 852, 993, 1035, 1175, 1277
HpySE526I ACGT 1 cut(s) 631
Hsp92II CATG 5 cut(s) 104, 154, 629, 963, 987
Kzo9I GATC 2 cut(s) 902, 1082
LguI GCTCTTC 1 cut(s) 784
LmnI GCTCC 3 cut(s) 118, 350, 593
Lsp1109I GCAGC 5 cut(s) 181, 291, 469, 1054, 1111
LweI GCATC 5 cut(s) 406, 832, 1016, 1029, 1054
MaeI CTAG 8 cut(s) 78, 129, 342, 432, 489, 953, 1058, 1440
MaeII ACGT 1 cut(s) 631
MaeIII GTNAC 6 cut(s) 200, 337, 919, 1053, 1135, 1469
MalI GATC 2 cut(s) 904, 1084
MboI GATC 2 cut(s) 902, 1082
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 1 cut(s) 1082
MhlI GDGCHC 5 cut(s) 96, 115, 389, 538, 1496
MlsI TGGCCA 1 cut(s) 1320
MluCI AATT 9 cut(s) 219, 259, 366, 492, 652, 846, 1369, 1448, 1519
MluNI TGGCCA 1 cut(s) 1320
MlyI GAGTC 2 cut(s) 436, 1109
Mox20I TGGCCA 1 cut(s) 1320
MroXI GAANNNNTTC 1 cut(s) 1071
MscI TGGCCA 1 cut(s) 1320
MseI TTAA 3 cut(s) 369, 1248, 1518
MslI CAYNNNNRTG 1 cut(s) 99
Msp20I TGGCCA 1 cut(s) 1320
MspI CCGG 1 cut(s) 569
MspR9I CCNGG 1 cut(s) 504
MunI CAATTG 1 cut(s) 219
Mva1269I GAATGC 1 cut(s) 457
MvaI CCWGG 1 cut(s) 504
MvnI CGCG 2 cut(s) 165, 460
MwoI GCNNNNNNNGC 5 cut(s) 106, 188, 301, 533, 1130
NdeII GATC 2 cut(s) 902, 1082
NlaIII CATG 5 cut(s) 104, 154, 629, 963, 987
NlaIV GGNNCC 6 cut(s) 318, 352, 535, 866, 1144, 1546
NmeAIII GCCGAG 1 cut(s) 379
NmuCI GTSAC 4 cut(s) 200, 337, 919, 1053
NruI TCGCGA 1 cut(s) 460
NspI RCATGY 1 cut(s) 629
PaeI GCATGC 1 cut(s) 629
PaeR7I CTCGAG 1 cut(s) 388
PceI AGGCCT 1 cut(s) 295
PciSI GCTCTTC 1 cut(s) 784
PctI GAATGC 1 cut(s) 457
PdmI GAANNNNTTC 1 cut(s) 1071
PfeI GAWTC 4 cut(s) 73, 146, 710, 1072
PkrI GCNGC 5 cut(s) 171, 306, 484, 1044, 1126
PleI GAGTC 2 cut(s) 435, 1109
PpsI GAGTC 2 cut(s) 435, 1109
PshAI GACNNNNGTC 1 cut(s) 511
PsiI TTATAA 1 cut(s) 1221
Psp6I CCWGG 1 cut(s) 502
PspGI CCWGG 1 cut(s) 502
PspN4I GGNNCC 6 cut(s) 318, 352, 535, 866, 1144, 1546
PspPI GGNCC 4 cut(s) 317, 1143, 1281, 1544
PstI CTGCAG 2 cut(s) 309, 513
PstNI CAGNNNCTG 2 cut(s) 304, 884
PsuI RGATCY 1 cut(s) 1082
RruI TCGCGA 1 cut(s) 460
RseI CAYNNNNRTG 1 cut(s) 99
SapI GCTCTTC 1 cut(s) 784
SaqAI TTAA 3 cut(s) 369, 1248, 1518
SatI GCNGC 5 cut(s) 170, 305, 483, 1043, 1125
Sau3AI GATC 2 cut(s) 902, 1082
Sau96I GGNCC 4 cut(s) 317, 1143, 1281, 1544
SchI GAGTC 2 cut(s) 436, 1109
ScrFI CCNGG 1 cut(s) 504
SduI GDGCHC 5 cut(s) 96, 115, 389, 538, 1496
SfaNI GCATC 5 cut(s) 406, 832, 1016, 1029, 1054
SfcI CTRYAG 3 cut(s) 232, 305, 509
Sfr274I CTCGAG 1 cut(s) 388
SinI GGWCC 4 cut(s) 317, 1143, 1281, 1544
SlaI CTCGAG 1 cut(s) 388
SmiMI CAYNNNNRTG 1 cut(s) 99
SmlI CTYRAG 1 cut(s) 388
SmoI CTYRAG 1 cut(s) 388
SphI GCATGC 1 cut(s) 629
Sse9I AATT 9 cut(s) 219, 259, 366, 492, 652, 846, 1369, 1448, 1519
SseBI AGGCCT 1 cut(s) 295
SspMI CTAG 8 cut(s) 78, 129, 342, 432, 489, 953, 1058, 1440
StuI AGGCCT 1 cut(s) 295
StyD4I CCNGG 1 cut(s) 502
StyI CCWWGG 3 cut(s) 431, 907, 1193
TaaI ACNGT 5 cut(s) 41, 206, 233, 321, 601
TaiI ACGT 1 cut(s) 634
TaqI TCGA 4 cut(s) 389, 782, 1013, 1048
TasI AATT 9 cut(s) 219, 259, 366, 492, 652, 846, 1369, 1448, 1519
TfiI GAWTC 4 cut(s) 73, 146, 710, 1072
Tru1I TTAA 3 cut(s) 369, 1248, 1518
Tru9I TTAA 3 cut(s) 369, 1248, 1518
TscAI CASTG 5 cut(s) 46, 211, 342, 426, 1501
TseFI GTSAC 4 cut(s) 200, 337, 919, 1053
TseI GCWGC 5 cut(s) 169, 304, 482, 1042, 1124
Tsp45I GTSAC 4 cut(s) 200, 337, 919, 1053
TspDTI ATGAA 7 cut(s) 48, 117, 948, 1226, 1406, 1439, 1497
TspGWI ACGGA 1 cut(s) 1306
TspRI CASTG 5 cut(s) 46, 211, 342, 426, 1501
VpaK11BI GGWCC 4 cut(s) 317, 1143, 1281, 1544
XapI RAATTY 2 cut(s) 259, 492
XceI RCATGY 1 cut(s) 629
XhoI CTCGAG 1 cut(s) 388
XmaJI CCTAGG 1 cut(s) 431
XmiI GTMKAC 1 cut(s) 515
XmnI GAANNNNTTC 1 cut(s) 1071
XspI CTAG 8 cut(s) 78, 129, 342, 432, 489, 953, 1058, 1440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.