Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0011441

Physical Location & Seq

Genomic Coordinates Forward (+)

17383928 .. 17389302

Gene Structure 5375 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom09g16950.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2856 bp

ATGAATGATTCTGCAGGGAAAACGGCGTCGTCTTTGGTGATAGCAGAGAAGAAGACTCATAGGTCGGGTGGTTGCGTTGGCATTTTCTTCCAGCTCCTTGACTGGAACCGCCGGTTCGCCAAGAAGAAGCTCTTCTCCAAGAAATTGCTTCCTCCATCTCGAGCAAACAAGGTTTCCAAGAAGTTCAGAGATGAAAAAATGCCGACTTCCAAGCTACATTTGATTGCTGATGAAAACAGTGGGGGTTTCCCAAATGCGAAGAAAAACGTGAATCGAAGTGTTGATTATGAGCACAAGCATGAACTGTGTGCTCCGAGTTTGGTTGCTAGGCTTATGGGTCTTGAATCTATGCCTGCCACGCCCGAGAAGCCGATGACAGGTTCATTTGCTGATGTTAGTGACAGTGGAGAGAAGACAATTGTGAATAACTGTAGTGGTAATGATAGAGAAGAACTGAATTTGGAGACACAAAATGGAAAGTCTGAAGCAAGGCCTCAGAAGCTGCAGAAAATGGGACCGTATGAGAAGAGGGCAGTGACTAGGTTTGGAGCTGAGGCACTTCAAATTAAGAGTGTTTTGTCGTGCTCGAGGAAGCATCACCCAAAGCTTGCTTCTCCAGTGAAGAGTCCGAGGATTTCCTCTGGGAAGAATGCTTCGCGAACCTCTCGGTTGATTGGTGCTGCTACTAGAATTTTAGAACCTGGACTGCAGTCTACGAATAGAGCAAAAGGTGCCATCACTTACTCGCCTTCGTTTCAATATCCTCCGGTGCGTGAGGTTGTGGAAGATGGAGCAACAATTCAATCACCAGAGATTTCTAAGCATGCATGTTATAATGCTGGTGCGTCGAATTCTCTGATAGGCCAAACTTCTTGCAAAAGCTGTGGTAGTTTAGTAGATGTTGTGGAATCATCGTCAAAGGTGGAGGCACAACAACCTGCTTTCCCATCCTTTGCTTCAAACATTGTCAATGGCTCCTCGATGGTAGTAGAACAAAGTAAGCCAAAGTCATCCATATCCTCCTTTGGGAAAGAAAAGGATGCAATTTCTCGGGGAACTCGGAACCAACCTGTATCAGTTTCTGGTCAAAAGGGAATACGATCCCTTGGTGAACCTGTCACAGAAGGGAAGTCTCTACCACCAGAAGGTCTAGCTTCATGGCAGTTGTCGTCAAGTCAACCATGCAAACCTCAGAAGGGAGAAGCATCTTCGATTGCCTCAAAGAATAGGTCTCAGATGCAGTATCGAATGTCACTAGGAAGAGAAAGGATTCCACCCAGATCCAAACTAAATAGTCTTGATGGAAGAAGAGCCTCATCTGCTGCCAATGCTGTTACGGGGACCAAAGACTTTGTTGCCTTAAACCGAAATCTGAGTGGTCGTTCCCGACCAAGGGTGCCTATCAAAATGAACGATTTTAAGTTTGACACAGAGAAGGAAGCTTTTACTGGAAAAGATGATTACCCATCACAGCTAAGGACCACAATACGGAAAAGGAGGATTATCAATGGTAGTGGCCAAGTTGAAAGTACTGGTTTTGTTAGTTCAACTTCCACAAGACGGGGGAATTATCAGTTTGATGTGCCAACTGGAAAAAGATTAGGAAATGGTGTTCATTTTATGAACCACACTTCTGCTAGGAATAAATTAGCGGGTCAGAGAGAACATAACAGAGCAAATGGAAATAGGGGCACTGATGTTATTTCATTCACATTTAACTCCCCTATCGGGAACAGGACTGGAAACCCTGCAGGAATGCAAGGTACAAACACAGACAATTGCATAAAGGCACCTCCCCAAGAACCATTGCCATTGAGTGCAGATGTTGTTGGTGCCTTTCTGGAACAAAAACTGAGGGAGCTTGCTTGTCAAGAAGATGAAGACATGGCAACAGGTGCTTCATCCAAGAGGTCCACTGCTATGATTCTCCAAGAGCTAATATCTGCCTTGTCTGCAGATCATTCTCTTTCTTCTCATGACGGACAGATGGCCAATACAGATATAGCGTCACCTGCTTGTGGAAAGATAGATAGATCAGTTGGAATCTTTCATGATGGTCATCATTTGAGCCCTGGGTCGGTTCTTGAAGCTTCATTTTCTTCTAGTAGTCTGGATGATAGCTCAGGGCATAGGTCCTTCTATCCTCACTCTATGGATTACTCTGCTGATCAGTTGCAACTTGGACATGATGCTATTGCTGACATTGTAGACTCTGCCACTTCAGTGGACAAAAATAAGACTGGCGGTGAGATGATAACTGCTCTTTTCAGCAATGTATCGAGAATGTTGCATAGCATCGATGCTGGTGGGGAAAGATTGAGTGGAGGCAAGCTTACCAATGCAAATGAAGTTATCTTGAATGCTGAACTGTTGTTTGGAGATGTGACTCTTCACAATAAGGGGAACATAATGGAAGGTCTTTTCATCAGTCCCTTGCTTCTTGATCTGGAAACTATTGCTGGCACCATGATGACGAAATTCCATGTGTATAGTAGTTTCAGGGGCGTCAAAGAACTTACTGAATTCCTATTTGACTTTGTCGTTGAGTACTTGGAATCAAAATATGACCGATACTGCAACTCCGGATTCAGATTTTGGAAAAAACTCCCCGCAGGCATGAACCACAAGTTGATGATTCAAGAGGTTCGGGAGGAGATTCAGAAGTGGACGGATTTGGCTGGAATGATTCCTGATGAGTTCATAGAATGGGATATGAGTCATTCCCTGGGGAAGTGGATGGATTTTAATATCGAAGCGTTTGAGGTCGGTTCTGAAATTGACGGAGACATTCTTCAAAGTTTGGTTAATGAAGTTGTGATCGACCTCTGCGAGTGCAGGCTGCTGGATTCCTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4378 VARLMGL

Protein Analysis

952

Amino Acids

103.88

Weight (kDa)

8.9

Isoelectric Point (pI)

46.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
VARLMGL	PF14383	94 - 120	8.5e-11	DUF761-associated sequence motif
DUF4378	PF14309	840 - 937	2.6e-18	Domain of unknown function (DUF4378)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011441)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67040 AT1G67040
malus_domestica	MD09G1253500.v1.1 MD09G1253600.v1.1 MD17G1245400.v1.1
prunus_persica	Prupe.3G143500_v2.0.a1
pyrus_communis	pycom09g16950 pycom17g24780 pycom17g24790
rosa_chinensis	RchiOBHm_Chr2g0126071
rosa_laevigata	RLG00000018886
rosa_multiflora	Rmu_sc0001506.1_g000003
rosa_roxburghii	Rroxscaffold_2G00117460
rosa_rugosa	Rorug02G0260600 Rorug02G0260700
rosa_samantha	Rh2AG318100 Rh2BG326500 Rh2DG344500 Rh2DG344600
rosa_wichuraiana	Rw2G025760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	834
AarI	CACCTGC	1 cut(s)	2020
AasI	GACNNNNNNGTC	1 cut(s)	61
Acc36I	ACCTGC	2 cut(s)	946, 2020
AccB1I	GGYRCC	5 cut(s)	731, 1396, 1789, 1832, 2462
AccI	GTMKAC	2 cut(s)	713, 2208
AccII	CGCG	1 cut(s)	658
AccIII	TCCGGA	1 cut(s)	2582
AciI	CCGC	4 cut(s)	109, 1652, 2244, 2610
AclWI	GGATC	2 cut(s)	1095, 1275
AcoI	YGGCCR	2 cut(s)	1516, 1989
AcsI	RAATTY	5 cut(s)	457, 690, 850, 2477, 2522
AcuI	CTGAAG	2 cut(s)	504, 2205
AcyI	GRCGYC	2 cut(s)	26, 2505
AfaI	GTAC	3 cut(s)	1531, 1765, 2549
AjnI	CCWGG	3 cut(s)	700, 2071, 2724
AjuI	GAANNNNNNNTTGG	4 cut(s)	595, 627, 2358, 2390
AleI	CACNNNNGTG	1 cut(s)	2222
AloI	GAACNNNNNNTCC	2 cut(s)	1596, 1628
Alw21I	GWGCWC	3 cut(s)	294, 313, 587
Alw26I	GTCTC	4 cut(s)	458, 1137, 1236, 2778
AlwI	GGATC	2 cut(s)	1095, 1275
AlwNI	CAGNNNCTG	2 cut(s)	502, 1082
Ama87I	CYCGRG	4 cut(s)	159, 362, 586, 1050
Aor13HI	TCCGGA	1 cut(s)	2582
AoxI	GGCC	4 cut(s)	491, 862, 1516, 1989
ApeKI	GCWGC	4 cut(s)	502, 680, 1322, 2839
ApoI	RAATTY	5 cut(s)	457, 690, 850, 2477, 2522
ArsI	GACNNNNNNTTYG	2 cut(s)	1214, 1246
Asp700I	GAANNNNTTC	2 cut(s)	131, 1269
AspS9I	GGNCC	5 cut(s)	515, 1341, 1479, 1911, 2133
AsuHPI	GGTGA	6 cut(s)	49, 590, 798, 1121, 2001, 2258
AvaI	CYCGRG	4 cut(s)	159, 362, 586, 1050
AvaII	GGWCC	5 cut(s)	515, 1341, 1479, 1911, 2133
BaeGI	GKGCMC	1 cut(s)	1694
BalI	TGGCCA	2 cut(s)	1518, 1991
BanI	GGYRCC	5 cut(s)	731, 1396, 1789, 1832, 2462
BanII	GRGCYC	1 cut(s)	2072
BbsI	GAAGAC	3 cut(s)	59, 419, 1887
Bbv12I	GWGCWC	3 cut(s)	294, 313, 587
BbvCI	CCTCAGC	1 cut(s)	552
BbvI	GCAGC	4 cut(s)	489, 667, 1309, 2826
BceAI	ACGGC	1 cut(s)	39
BcgI	CGANNNNNNTGC	2 cut(s)	2269, 2303
BciT130I	CCWGG	3 cut(s)	702, 2073, 2726
BclI	TGATCA	1 cut(s)	2167
BcoDI	GTCTC	4 cut(s)	458, 1137, 1236, 2778
BfaI	CTAG	7 cut(s)	327, 540, 687, 1151, 1256, 1638, 2103
BfmI	CTRYAG	6 cut(s)	12, 430, 503, 707, 1749, 1953
BfuAI	ACCTGC	2 cut(s)	946, 2020
BisI	GCNGC	4 cut(s)	503, 681, 1323, 2840
BlsI	GCNGC	4 cut(s)	504, 682, 1324, 2841
BmcAI	AGTACT	2 cut(s)	1531, 2549
Bme1390I	CCNGG	3 cut(s)	702, 2073, 2726
Bme18I	GGWCC	5 cut(s)	515, 1341, 1479, 1911, 2133
BmeT110I	CYCGRG	4 cut(s)	159, 362, 586, 1050
BmgT120I	GGNCC	5 cut(s)	515, 1341, 1479, 1911, 2133
BmrFI	CCNGG	3 cut(s)	702, 2073, 2726
BmsI	GCATC	7 cut(s)	604, 1030, 1214, 1227, 2179, 2290, 2304
BoxI	GACNNNNGTC	1 cut(s)	709
BpiI	GAAGAC	3 cut(s)	59, 419, 1887
BpmI	CTGGAG	1 cut(s)	600
Bpu10I	CCTNAGC	3 cut(s)	552, 1475, 2122
Bsa29I	ATCGAT	1 cut(s)	2298
BsaBI	GATNNNNATC	1 cut(s)	2058
BsaHI	GRCGYC	2 cut(s)	26, 2505
BsaI	GGTCTC	1 cut(s)	1236
BsaJI	CCNNGG	7 cut(s)	629, 1105, 1391, 2071, 2072, 2724, 2725
BsaWI	WCCGGW	2 cut(s)	766, 2582
BsaXI	ACNNNNNCTCC	2 cut(s)	2564, 2594
Bse118I	RCCGGY	1 cut(s)	111
Bse1I	ACTGG	7 cut(s)	107, 617, 1453, 1537, 1594, 1744, 2245
Bse3DI	GCAATG	2 cut(s)	1805, 2278
Bse8I	GATNNNNATC	1 cut(s)	2058
BseAI	TCCGGA	1 cut(s)	2582
BseBI	CCWGG	3 cut(s)	702, 2073, 2726
BseCI	ATCGAT	1 cut(s)	2298
BseDI	CCNNGG	7 cut(s)	629, 1105, 1391, 2071, 2072, 2724, 2725
BseGI	GGATG	6 cut(s)	947, 1010, 1045, 1901, 2119, 2742
BseJI	GATNNNNATC	1 cut(s)	2058
BseMI	GCAATG	2 cut(s)	1805, 2278
BseMII	CTCAG	7 cut(s)	509, 543, 1205, 1247, 1364, 1844, 2136
BseNI	ACTGG	7 cut(s)	107, 617, 1453, 1537, 1594, 1744, 2245
BseRI	GAGGAG	2 cut(s)	967, 2666
BseSI	GKGCMC	1 cut(s)	1694
BseXI	GCAGC	4 cut(s)	489, 667, 1309, 2826
BsgI	GTGCAG	2 cut(s)	1839, 2854
Bsh1236I	CGCG	1 cut(s)	658
BshFI	GGCC	4 cut(s)	493, 864, 1518, 1991
BshNI	GGYRCC	5 cut(s)	731, 1396, 1789, 1832, 2462
BshVI	ATCGAT	1 cut(s)	2298
BsiHKAI	GWGCWC	3 cut(s)	294, 313, 587
BsiHKCI	CYCGRG	4 cut(s)	159, 362, 586, 1050
BsiSI	CCGG	3 cut(s)	112, 767, 2583
BslFI	GGGAC	3 cut(s)	528, 1354, 2415
BsmAI	GTCTC	4 cut(s)	458, 1137, 1236, 2778
BsmFI	GGGAC	3 cut(s)	528, 1354, 2415
BsmI	GAATGC	3 cut(s)	655, 1761, 2365
BsnI	GGCC	4 cut(s)	493, 864, 1518, 1991
Bso31I	GGTCTC	1 cut(s)	1236
BsoBI	CYCGRG	4 cut(s)	159, 362, 586, 1050
Bsp1286I	GDGCHC	5 cut(s)	294, 313, 587, 1694, 2072
Bsp13I	TCCGGA	1 cut(s)	2582
Bsp143I	GATC	7 cut(s)	1100, 1280, 1957, 2033, 2167, 2443, 2817
Bsp68I	TCGCGA	1 cut(s)	658
BspACI	CCGC	4 cut(s)	109, 1652, 2244, 2610
BspANI	GGCC	4 cut(s)	493, 864, 1518, 1991
BspCNI	CTCAG	7 cut(s)	508, 544, 1204, 1246, 1365, 1845, 2135
BspDI	ATCGAT	1 cut(s)	2298
BspEI	TCCGGA	1 cut(s)	2582
BspFNI	CGCG	1 cut(s)	658
BspHI	TCATGA	2 cut(s)	1975, 2050
BspMAI	CTGCAG	5 cut(s)	16, 507, 711, 1753, 1957
BspMI	ACCTGC	2 cut(s)	946, 2020
BspPI	GGATC	2 cut(s)	1095, 1275
BspQI	GCTCTTC	2 cut(s)	137, 1303
BspT107I	GGYRCC	5 cut(s)	731, 1396, 1789, 1832, 2462
BspTNI	GGTCTC	1 cut(s)	1236
BsrDI	GCAATG	2 cut(s)	1805, 2278
BsrFI	RCCGGY	1 cut(s)	111
BsrI	ACTGG	7 cut(s)	107, 617, 1453, 1537, 1594, 1744, 2245
BssAI	RCCGGY	1 cut(s)	111
BssECI	CCNNGG	7 cut(s)	629, 1105, 1391, 2071, 2072, 2724, 2725
BssMI	GATC	7 cut(s)	1100, 1280, 1957, 2033, 2167, 2443, 2817
BssNI	GRCGYC	2 cut(s)	26, 2505
BssT1I	CCWWGG	2 cut(s)	1105, 1391
Bst2UI	CCWGG	3 cut(s)	702, 2073, 2726
Bst4CI	ACNGT	6 cut(s)	239, 306, 404, 431, 519, 2370
Bst6I	CTCTTC	6 cut(s)	137, 521, 617, 1255, 1303, 2394
BstACI	GRCGYC	2 cut(s)	26, 2505
BstAPI	GCANNNNNTGC	2 cut(s)	731, 1895
BstC8I	GCNNGC	8 cut(s)	354, 609, 825, 1863, 2330, 2461, 2614, 2837
BstDEI	CTNAG	9 cut(s)	495, 552, 819, 1191, 1233, 1373, 1475, 1853, 2122
BstF5I	GGATG	6 cut(s)	947, 1010, 1045, 1901, 2119, 2742
BstFNI	CGCG	1 cut(s)	658
BstKTI	GATC	7 cut(s)	1103, 1283, 1960, 2036, 2170, 2446, 2820
BstMAI	GTCTC	4 cut(s)	458, 1137, 1236, 2778
BstMBI	GATC	7 cut(s)	1100, 1280, 1957, 2033, 2167, 2443, 2817
BstMWI	GCNNNNNNNGC	9 cut(s)	358, 367, 499, 731, 1319, 1328, 1895, 1952, 2012
BstNI	CCWGG	3 cut(s)	702, 2073, 2726
BstNSI	RCATGY	2 cut(s)	827, 831
BstPAI	GACNNNNGTC	1 cut(s)	709
BstSCI	CCNGG	3 cut(s)	700, 2071, 2724
BstSFI	CTRYAG	6 cut(s)	12, 430, 503, 707, 1749, 1953
BstSLI	GKGCMC	1 cut(s)	1694
BstUI	CGCG	1 cut(s)	658
BstV1I	GCAGC	4 cut(s)	489, 667, 1309, 2826
BstV2I	GAAGAC	3 cut(s)	59, 419, 1887
BstX2I	RGATCY	1 cut(s)	1280
BstXI	CCANNNNNNTGG	1 cut(s)	2224
BstYI	RGATCY	1 cut(s)	1280
Bsu15I	ATCGAT	1 cut(s)	2298
BsuRI	GGCC	4 cut(s)	493, 864, 1518, 1991
BsuTUI	ATCGAT	1 cut(s)	2298
BtsCI	GGATG	6 cut(s)	947, 1010, 1045, 1901, 2119, 2742
BtsI	GCAGTG	2 cut(s)	540, 1914
BtsIMutI	CAGTG	7 cut(s)	244, 409, 540, 624, 1692, 1914, 2229
BtuMI	TCGCGA	1 cut(s)	658
BveI	ACCTGC	2 cut(s)	946, 2020
Cac8I	GCNNGC	8 cut(s)	354, 609, 825, 1863, 2330, 2461, 2614, 2837
CaiI	CAGNNNCTG	2 cut(s)	502, 1082
CciI	TCATGA	2 cut(s)	1975, 2050
Cfr10I	RCCGGY	1 cut(s)	111
Cfr13I	GGNCC	5 cut(s)	515, 1341, 1479, 1911, 2133
ClaI	ATCGAT	1 cut(s)	2298
CseI	GACGC	4 cut(s)	15, 834, 1995, 2494
Csp6I	GTAC	3 cut(s)	1530, 1764, 2548
CspCI	CAANNNNNGTGG	4 cut(s)	865, 900, 1495, 1530
CviQI	GTAC	3 cut(s)	1530, 1764, 2548
DdeI	CTNAG	9 cut(s)	495, 552, 819, 1191, 1233, 1373, 1475, 1853, 2122
DpnI	GATC	7 cut(s)	1102, 1282, 1959, 2035, 2169, 2445, 2819
DpnII	GATC	7 cut(s)	1100, 1280, 1957, 2033, 2167, 2443, 2817
DrdI	GACNNNNNNGTC	1 cut(s)	61
DseDI	GACNNNNNNGTC	1 cut(s)	61
EaeI	YGGCCR	2 cut(s)	1516, 1989
Eam1104I	CTCTTC	6 cut(s)	137, 521, 617, 1255, 1303, 2394
EarI	CTCTTC	6 cut(s)	137, 521, 617, 1255, 1303, 2394
Eco130I	CCWWGG	2 cut(s)	1105, 1391
Eco147I	AGGCCT	1 cut(s)	493
Eco24I	GRGCYC	1 cut(s)	2072
Eco31I	GGTCTC	1 cut(s)	1236
Eco47I	GGWCC	5 cut(s)	515, 1341, 1479, 1911, 2133
Eco57I	CTGAAG	2 cut(s)	504, 2205
Eco88I	CYCGRG	4 cut(s)	159, 362, 586, 1050
EcoO109I	RGGNCCY	1 cut(s)	2133
EcoRI	GAATTC	2 cut(s)	850, 2522
EcoRII	CCWGG	3 cut(s)	700, 2071, 2724
EcoT14I	CCWWGG	2 cut(s)	1105, 1391
EcoT22I	ATGCAT	1 cut(s)	829
EcoT38I	GRGCYC	1 cut(s)	2072
ErhI	CCWWGG	2 cut(s)	1105, 1391
FalI	AAGNNNNNCTT	2 cut(s)	116, 148
FaqI	GGGAC	3 cut(s)	528, 1354, 2415
FauI	CCCGC	2 cut(s)	1645, 2617
FbaI	TGATCA	1 cut(s)	2167
FblI	GTMKAC	2 cut(s)	713, 2208
Fnu4HI	GCNGC	4 cut(s)	503, 681, 1323, 2840
FokI	GGATG	6 cut(s)	934, 997, 1052, 1888, 2126, 2749
FriOI	GRGCYC	1 cut(s)	2072
Fsp4HI	GCNGC	4 cut(s)	503, 681, 1323, 2840
FspBI	CTAG	7 cut(s)	327, 540, 687, 1151, 1256, 1638, 2103
GluI	GCNGC	4 cut(s)	503, 681, 1323, 2840
GsuI	CTGGAG	1 cut(s)	600
HaeIII	GGCC	4 cut(s)	493, 864, 1518, 1991
HapII	CCGG	3 cut(s)	112, 767, 2583
HgaI	GACGC	4 cut(s)	15, 834, 1995, 2494
Hin1I	GRCGYC	2 cut(s)	26, 2505
HincII	GTYRAC	1 cut(s)	1178
HindII	GTYRAC	1 cut(s)	1178
HindIII	AAGCTT	4 cut(s)	605, 1440, 2088, 2330
HpaII	CCGG	3 cut(s)	112, 767, 2583
HphI	GGTGA	6 cut(s)	49, 590, 798, 1121, 2001, 2258
Hpy166II	GTNNAC	7 cut(s)	714, 1112, 1178, 1914, 2209, 2227, 2667
Hpy8I	GTNNAC	7 cut(s)	714, 1112, 1178, 1914, 2209, 2227, 2667
Hpy99I	CGWCG	2 cut(s)	31, 850
HpyAV	CCTTC	7 cut(s)	759, 1118, 1139, 1189, 1429, 2146, 2408
HpyCH4III	ACNGT	6 cut(s)	239, 306, 404, 431, 519, 2370
HpyCH4IV	ACGT	1 cut(s)	267
HpyF10VI	GCNNNNNNNGC	9 cut(s)	358, 367, 499, 731, 1319, 1328, 1895, 1952, 2012
HpyF3I	CTNAG	9 cut(s)	495, 552, 819, 1191, 1233, 1373, 1475, 1853, 2122
HpySE526I	ACGT	1 cut(s)	267
Hsp92I	GRCGYC	2 cut(s)	26, 2505
Kpn2I	TCCGGA	1 cut(s)	2582
Ksp22I	TGATCA	1 cut(s)	2167
Kzo9I	GATC	7 cut(s)	1100, 1280, 1957, 2033, 2167, 2443, 2817
LguI	GCTCTTC	2 cut(s)	137, 1303
LmnI	GCTCC	6 cut(s)	99, 316, 548, 791, 980, 1858
Lsp1109I	GCAGC	4 cut(s)	489, 667, 1309, 2826
LweI	GCATC	7 cut(s)	604, 1030, 1214, 1227, 2179, 2290, 2304
MaeI	CTAG	7 cut(s)	327, 540, 687, 1151, 1256, 1638, 2103
MaeII	ACGT	1 cut(s)	267
MaeIII	GTNAC	7 cut(s)	398, 535, 1117, 1251, 1333, 2007, 2383
MalI	GATC	7 cut(s)	1102, 1282, 1959, 2035, 2169, 2445, 2819
MboI	GATC	7 cut(s)	1100, 1280, 1957, 2033, 2167, 2443, 2817
MfeI	CAATTG	2 cut(s)	417, 1777
MflI	RGATCY	1 cut(s)	1280
MhlI	GDGCHC	5 cut(s)	294, 313, 587, 1694, 2072
MlsI	TGGCCA	2 cut(s)	1518, 1991
MluNI	TGGCCA	2 cut(s)	1518, 1991
MlyI	GAGTC	5 cut(s)	49, 634, 2204, 2380, 2725
MmeI	TCCRAC	1 cut(s)	2020
Mox20I	TGGCCA	2 cut(s)	1518, 1991
Mph1103I	ATGCAT	1 cut(s)	829
MroI	TCCGGA	1 cut(s)	2582
MroXI	GAANNNNTTC	2 cut(s)	131, 1269
MscI	TGGCCA	2 cut(s)	1518, 1991
MseI	TTAA	6 cut(s)	567, 1361, 1419, 1716, 2745, 2805
MslI	CAYNNNNRTG	4 cut(s)	297, 1919, 2222, 2468
Msp20I	TGGCCA	2 cut(s)	1518, 1991
MspI	CCGG	3 cut(s)	112, 767, 2583
MspR9I	CCNGG	3 cut(s)	702, 2073, 2726
MunI	CAATTG	2 cut(s)	417, 1777
Mva1269I	GAATGC	3 cut(s)	655, 1761, 2365
MvaI	CCWGG	3 cut(s)	702, 2073, 2726
MvnI	CGCG	1 cut(s)	658
MwoI	GCNNNNNNNGC	9 cut(s)	358, 367, 499, 731, 1319, 1328, 1895, 1952, 2012
NdeII	GATC	7 cut(s)	1100, 1280, 1957, 2033, 2167, 2443, 2817
NmuCI	GTSAC	6 cut(s)	398, 535, 1117, 1251, 2007, 2383
NruI	TCGCGA	1 cut(s)	658
NsiI	ATGCAT	1 cut(s)	829
NspI	RCATGY	2 cut(s)	827, 831
OliI	CACNNNNGTG	1 cut(s)	2222
PaeI	GCATGC	1 cut(s)	827
PaeR7I	CTCGAG	2 cut(s)	159, 586
PagI	TCATGA	2 cut(s)	1975, 2050
PaqCI	CACCTGC	1 cut(s)	2020
PasI	CCCWGGG	2 cut(s)	2072, 2725
PceI	AGGCCT	1 cut(s)	493
PciSI	GCTCTTC	2 cut(s)	137, 1303
PcsI	WCGNNNNNNNCGW	1 cut(s)	2826
PctI	GAATGC	3 cut(s)	655, 1761, 2365
PdmI	GAANNNNTTC	2 cut(s)	131, 1269
PflFI	GACNNNGTC	1 cut(s)	2537
PkrI	GCNGC	4 cut(s)	504, 682, 1324, 2841
PleI	GAGTC	5 cut(s)	49, 633, 2204, 2380, 2724
PpsI	GAGTC	5 cut(s)	49, 633, 2204, 2380, 2724
PpuMI	RGGWCCY	1 cut(s)	2133
PshAI	GACNNNNGTC	1 cut(s)	709
PsiI	TTATAA	1 cut(s)	834
Psp5II	RGGWCCY	1 cut(s)	2133
Psp6I	CCWGG	3 cut(s)	700, 2071, 2724
PspGI	CCWGG	3 cut(s)	700, 2071, 2724
PspPI	GGNCC	5 cut(s)	515, 1341, 1479, 1911, 2133
PspPPI	RGGWCCY	1 cut(s)	2133
PspXI	VCTCGAGB	1 cut(s)	586
PstI	CTGCAG	5 cut(s)	16, 507, 711, 1753, 1957
PstNI	CAGNNNCTG	2 cut(s)	502, 1082
PsuI	RGATCY	1 cut(s)	1280
PsyI	GACNNNGTC	1 cut(s)	2537
RruI	TCGCGA	1 cut(s)	658
RsaI	GTAC	3 cut(s)	1531, 1765, 2549
RsaNI	GTAC	3 cut(s)	1530, 1764, 2548
RseI	CAYNNNNRTG	4 cut(s)	297, 1919, 2222, 2468
SapI	GCTCTTC	2 cut(s)	137, 1303
SaqAI	TTAA	6 cut(s)	567, 1361, 1419, 1716, 2745, 2805
SatI	GCNGC	4 cut(s)	503, 681, 1323, 2840
Sau3AI	GATC	7 cut(s)	1100, 1280, 1957, 2033, 2167, 2443, 2817
Sau96I	GGNCC	5 cut(s)	515, 1341, 1479, 1911, 2133
SbfI	CCTGCAGG	1 cut(s)	1753
ScaI	AGTACT	2 cut(s)	1531, 2549
SchI	GAGTC	5 cut(s)	49, 634, 2204, 2380, 2725
ScrFI	CCNGG	3 cut(s)	702, 2073, 2726
SdaI	CCTGCAGG	1 cut(s)	1753
SduI	GDGCHC	5 cut(s)	294, 313, 587, 1694, 2072
SfaNI	GCATC	7 cut(s)	604, 1030, 1214, 1227, 2179, 2290, 2304
SfcI	CTRYAG	6 cut(s)	12, 430, 503, 707, 1749, 1953
Sfr274I	CTCGAG	2 cut(s)	159, 586
SinI	GGWCC	5 cut(s)	515, 1341, 1479, 1911, 2133
SlaI	CTCGAG	2 cut(s)	159, 586
SmiMI	CAYNNNNRTG	4 cut(s)	297, 1919, 2222, 2468
SmlI	CTYRAG	2 cut(s)	159, 586
SmoI	CTYRAG	2 cut(s)	159, 586
SphI	GCATGC	1 cut(s)	827
Sse8387I	CCTGCAGG	1 cut(s)	1753
SseBI	AGGCCT	1 cut(s)	493
SsiI	CCGC	4 cut(s)	109, 1652, 2244, 2610
SspMI	CTAG	7 cut(s)	327, 540, 687, 1151, 1256, 1638, 2103
StuI	AGGCCT	1 cut(s)	493
StyD4I	CCNGG	3 cut(s)	700, 2071, 2724
StyI	CCWWGG	2 cut(s)	1105, 1391
TaaI	ACNGT	6 cut(s)	239, 306, 404, 431, 519, 2370
TaiI	ACGT	1 cut(s)	270
TaqII	GACCGA	1 cut(s)	2583
TatI	WGTACW	2 cut(s)	1529, 2547
Tru1I	TTAA	6 cut(s)	567, 1361, 1419, 1716, 2745, 2805
Tru9I	TTAA	6 cut(s)	567, 1361, 1419, 1716, 2745, 2805
TscAI	CASTG	7 cut(s)	244, 409, 540, 624, 1699, 1921, 2229
TseFI	GTSAC	6 cut(s)	398, 535, 1117, 1251, 2007, 2383
TseI	GCWGC	4 cut(s)	502, 680, 1322, 2839
Tsp45I	GTSAC	6 cut(s)	398, 535, 1117, 1251, 2007, 2383
TspGWI	ACGGA	4 cut(s)	1504, 1995, 2684, 2796
TspRI	CASTG	7 cut(s)	244, 409, 540, 624, 1699, 1921, 2229
Tth111I	GACNNNGTC	1 cut(s)	2537
VpaK11BI	GGWCC	5 cut(s)	515, 1341, 1479, 1911, 2133
XapI	RAATTY	5 cut(s)	457, 690, 850, 2477, 2522
XceI	RCATGY	2 cut(s)	827, 831
XhoI	CTCGAG	2 cut(s)	159, 586
XmiI	GTMKAC	2 cut(s)	713, 2208
XmnI	GAANNNNTTC	2 cut(s)	131, 1269
XspI	CTAG	7 cut(s)	327, 540, 687, 1151, 1256, 1638, 2103
ZrmI	AGTACT	2 cut(s)	1531, 2549
Zsp2I	ATGCAT	1 cut(s)	829

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.