MD09G1253600.v1.1

DUF761-associated sequence motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011441
Physical Location & Seq
Forward (+)
32470231 .. 32473997
3767 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1253600.v1.1.491

Sequence Viewer

Length: 930 bp
ATGATTCTCCAAGAGCTAATATCTGCCTTGTCTGCAGATCATTCTAATTCTTCTCATGACGGACAGATGGCCAATACAATCCAGAGGTCCACTGCTTGTGGAAAGACAGATAGATCAGTTGGAATCTTTCATGATGGTCATCATTTGAGCCCTGGGTCGGTTCTTGAAGCTTCATTTTCTTCGAGTAGTCTGGATGATAGCTCAGGGCATAGGTCCTTCTACCCTCACTCTATGGATTACTCTGCTGATCAGTTGCAACTTGGACATGATGCTGATATTGTAGACTCTGCCACTTCAGCGGACAAAAATAAGACTGGCGGTGAGATGATAACTGCTCTTTTCGGCAATGTATCAAGAATGTTGCATAGCATCGATGCTGGTGGGGAGAGATTGAGTGGAGGCAAGCTCACCAATGCAAATGAAGTTATCTTGAATGCCGAACTGTTGTTTGGAGATGTGACTCTTCACAATAAGGGGAACATAATGGAAGGTCTTTTCGTCAGTCCCTTGCTTCTTGATCTGGAAACTATTGCTGGCACCATGATGACGAAATTCCATGTGTATAGTAGTTTCAGGGGCGTCAAAGAACTTACTGAATTCCTATTTGGCTTTGTCGTTGAGTACTTGGAATCAAAATATGACCGATACTGCAACTCCGGATTCAGATTTTGGAAAAAACTCCCCGCAGGCATGAACCACAAGTTGATGATTCAAGAGGTTCGGGAGGAGATTCAGAAGTGGACGGATTTGGCTGGAATGATTCCTGATGAGTTGATAGAATGGGATATTAGTCATTCCCTGGGGAAGTGGACGGATTTTAATATCGAAGCGTTTGAGGTCGGTTCTGAAATTGACGGAGACATTCTTCAAAGTTTGGTTAATGAAGTTGTGATCGACCTCTGCGAGAGCAGGCTGCTGGATTCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 VARLMGL

Protein Analysis

310

Amino Acids

34.19

Weight (kDa)

4.78

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 198 - 295 9.4e-17 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 536
AccI GTMKAC 1 cut(s) 282
AccIII TCCGGA 1 cut(s) 656
AciI CCGC 3 cut(s) 299, 318, 684
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 2 cut(s) 551, 596
AcuI CTGAAG 1 cut(s) 279
AcyI GRCGYC 1 cut(s) 579
AfaI GTAC 1 cut(s) 623
AfiI CCNNNNNNNGG 1 cut(s) 157
AgsI TTSAA 4 cut(s) 167, 433, 713, 869
AjnI CCWGG 2 cut(s) 151, 798
AjuI GAANNNNNNNTTGG 4 cut(s) 432, 464, 588, 620
AluBI AGCT 4 cut(s) 16, 170, 201, 406
AluI AGCT 4 cut(s) 16, 170, 201, 406
Alw26I GTCTC 1 cut(s) 852
Aor13HI TCCGGA 1 cut(s) 656
AoxI GGCC 1 cut(s) 69
ApeKI GCWGC 1 cut(s) 913
ApoI RAATTY 2 cut(s) 551, 596
AspS9I GGNCC 2 cut(s) 87, 213
AsuHPI GGTGA 2 cut(s) 332, 400
AvaII GGWCC 2 cut(s) 87, 213
BalI TGGCCA 1 cut(s) 71
BanI GGYRCC 1 cut(s) 536
BanII GRGCYC 1 cut(s) 152
BbvI GCAGC 1 cut(s) 900
BccI CCATC 2 cut(s) 61, 128
BciT130I CCWGG 2 cut(s) 153, 800
BclI TGATCA 1 cut(s) 247
BcoDI GTCTC 1 cut(s) 852
BfmI CTRYAG 1 cut(s) 33
BisI GCNGC 1 cut(s) 914
BlsI GCNGC 1 cut(s) 915
BmcAI AGTACT 1 cut(s) 623
Bme1390I CCNGG 2 cut(s) 153, 800
Bme18I GGWCC 2 cut(s) 87, 213
BmgT120I GGNCC 2 cut(s) 87, 213
BmiI GGNNCC 1 cut(s) 538
BmrFI CCNGG 2 cut(s) 153, 800
BmsI GCATC 3 cut(s) 259, 364, 378
BplI GAGNNNNNCTC 2 cut(s) 390, 422
Bpu10I CCTNAGC 1 cut(s) 202
Bsa29I ATCGAT 1 cut(s) 372
BsaBI GATNNNNATC 1 cut(s) 138
BsaHI GRCGYC 1 cut(s) 579
BsaJI CCNNGG 4 cut(s) 151, 152, 798, 799
BsaWI WCCGGW 1 cut(s) 656
BsaXI ACNNNNNCTCC 2 cut(s) 638, 668
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 319
Bse3DI GCAATG 1 cut(s) 352
Bse8I GATNNNNATC 1 cut(s) 138
BseAI TCCGGA 1 cut(s) 656
BseBI CCWGG 2 cut(s) 153, 800
BseCI ATCGAT 1 cut(s) 372
BseDI CCNNGG 4 cut(s) 151, 152, 798, 799
BseGI GGATG 1 cut(s) 199
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 1 cut(s) 157
BseMI GCAATG 1 cut(s) 352
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 1 cut(s) 319
BseRI GAGGAG 1 cut(s) 740
BseXI GCAGC 1 cut(s) 900
BshFI GGCC 1 cut(s) 71
BshNI GGYRCC 1 cut(s) 536
BshVI ATCGAT 1 cut(s) 372
BsiSI CCGG 1 cut(s) 657
BslFI GGGAC 1 cut(s) 489
BslI CCNNNNNNNGG 1 cut(s) 157
BsmAI GTCTC 1 cut(s) 852
BsmFI GGGAC 1 cut(s) 489
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 152
Bsp13I TCCGGA 1 cut(s) 656
Bsp143I GATC 5 cut(s) 37, 113, 247, 517, 891
BspACI CCGC 3 cut(s) 299, 318, 684
BspANI GGCC 1 cut(s) 71
BspCNI CTCAG 1 cut(s) 215
BspDI ATCGAT 1 cut(s) 372
BspEI TCCGGA 1 cut(s) 656
BspHI TCATGA 2 cut(s) 55, 130
BspLI GGNNCC 1 cut(s) 538
BspMAI CTGCAG 1 cut(s) 37
BspT107I GGYRCC 1 cut(s) 536
BsrDI GCAATG 1 cut(s) 352
BsrI ACTGG 1 cut(s) 319
BssECI CCNNGG 4 cut(s) 151, 152, 798, 799
BssMI GATC 5 cut(s) 37, 113, 247, 517, 891
BssNI GRCGYC 1 cut(s) 579
Bst2UI CCWGG 2 cut(s) 153, 800
Bst4CI ACNGT 1 cut(s) 444
Bst6I CTCTTC 1 cut(s) 468
BstACI GRCGYC 1 cut(s) 579
BstC8I GCNNGC 4 cut(s) 404, 535, 688, 911
BstDEI CTNAG 1 cut(s) 202
BstF5I GGATG 1 cut(s) 199
BstKTI GATC 5 cut(s) 40, 116, 250, 520, 894
BstMAI GTCTC 1 cut(s) 852
BstMBI GATC 5 cut(s) 37, 113, 247, 517, 891
BstMWI GCNNNNNNNGC 2 cut(s) 32, 296
BstNI CCWGG 2 cut(s) 153, 800
BstSCI CCNGG 2 cut(s) 151, 798
BstSFI CTRYAG 1 cut(s) 33
BstV1I GCAGC 1 cut(s) 900
Bsu15I ATCGAT 1 cut(s) 372
BsuRI GGCC 1 cut(s) 71
BsuTUI ATCGAT 1 cut(s) 372
BtsCI GGATG 1 cut(s) 199
BtsI GCAGTG 1 cut(s) 90
BtsIMutI CAGTG 1 cut(s) 90
Cac8I GCNNGC 4 cut(s) 404, 535, 688, 911
CciI TCATGA 2 cut(s) 55, 130
Cfr13I GGNCC 2 cut(s) 87, 213
ClaI ATCGAT 1 cut(s) 372
CseI GACGC 1 cut(s) 568
Csp6I GTAC 1 cut(s) 622
CviAII CATG 6 cut(s) 56, 131, 266, 541, 557, 691
CviJI RGCY 9 cut(s) 16, 71, 150, 170, 201, 406, 609, 752, 913
CviKI_1 RGCY 9 cut(s) 16, 71, 150, 170, 201, 406, 609, 752, 913
CviQI GTAC 1 cut(s) 622
DdeI CTNAG 1 cut(s) 202
DpnI GATC 5 cut(s) 39, 115, 249, 519, 893
DpnII GATC 5 cut(s) 37, 113, 247, 517, 891
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
Eco24I GRGCYC 1 cut(s) 152
Eco47I GGWCC 2 cut(s) 87, 213
Eco57I CTGAAG 1 cut(s) 279
EcoO109I RGGNCCY 1 cut(s) 213
EcoRI GAATTC 1 cut(s) 596
EcoRII CCWGG 2 cut(s) 151, 798
EcoT38I GRGCYC 1 cut(s) 152
FaeI CATG 6 cut(s) 59, 134, 269, 544, 560, 694
FaqI GGGAC 1 cut(s) 489
FatI CATG 6 cut(s) 55, 130, 265, 540, 556, 690
FauI CCCGC 1 cut(s) 691
FbaI TGATCA 1 cut(s) 247
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 1 cut(s) 914
FokI GGATG 1 cut(s) 206
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 1 cut(s) 914
GluI GCNGC 1 cut(s) 914
HaeIII GGCC 1 cut(s) 71
HapII CCGG 1 cut(s) 657
HgaI GACGC 1 cut(s) 568
Hin1I GRCGYC 1 cut(s) 579
Hin1II CATG 6 cut(s) 59, 134, 269, 544, 560, 694
HindIII AAGCTT 1 cut(s) 168
HpaII CCGG 1 cut(s) 657
HphI GGTGA 2 cut(s) 332, 400
Hpy166II GTNNAC 4 cut(s) 90, 283, 741, 810
Hpy188I TCNGA 3 cut(s) 665, 735, 847
Hpy8I GTNNAC 4 cut(s) 90, 283, 741, 810
HpyAV CCTTC 2 cut(s) 226, 482
HpyCH4III ACNGT 1 cut(s) 444
HpyCH4V TGCA 5 cut(s) 35, 256, 364, 416, 651
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 296
HpyF3I CTNAG 1 cut(s) 202
Hsp92I GRCGYC 1 cut(s) 579
Hsp92II CATG 6 cut(s) 59, 134, 269, 544, 560, 694
Kpn2I TCCGGA 1 cut(s) 656
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 5 cut(s) 37, 113, 247, 517, 891
Lsp1109I GCAGC 1 cut(s) 900
LweI GCATC 3 cut(s) 259, 364, 378
MaeIII GTNAC 1 cut(s) 457
MalI GATC 5 cut(s) 39, 115, 249, 519, 893
MboI GATC 5 cut(s) 37, 113, 247, 517, 891
MboII GAAGA 4 cut(s) 42, 171, 455, 857
MhlI GDGCHC 1 cut(s) 152
MlsI TGGCCA 1 cut(s) 71
MluCI AATT 4 cut(s) 46, 551, 596, 849
MluNI TGGCCA 1 cut(s) 71
MlyI GAGTC 2 cut(s) 278, 454
MmeI TCCRAC 1 cut(s) 100
MnlI CCTC 7 cut(s) 78, 234, 392, 709, 718, 829, 908
Mox20I TGGCCA 1 cut(s) 71
MroI TCCGGA 1 cut(s) 656
MscI TGGCCA 1 cut(s) 71
MseI TTAA 2 cut(s) 819, 879
MslI CAYNNNNRTG 1 cut(s) 542
Msp20I TGGCCA 1 cut(s) 71
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 1 cut(s) 657
MspR9I CCNGG 2 cut(s) 153, 800
Mva1269I GAATGC 1 cut(s) 439
MvaI CCWGG 2 cut(s) 153, 800
MwoI GCNNNNNNNGC 2 cut(s) 32, 296
NdeII GATC 5 cut(s) 37, 113, 247, 517, 891
NlaIII CATG 6 cut(s) 59, 134, 269, 544, 560, 694
NlaIV GGNNCC 1 cut(s) 538
NmuCI GTSAC 1 cut(s) 457
PagI TCATGA 2 cut(s) 55, 130
PasI CCCWGGG 2 cut(s) 152, 799
PcsI WCGNNNNNNNCGW 1 cut(s) 900
PctI GAATGC 1 cut(s) 439
PfeI GAWTC 8 cut(s) 4, 123, 629, 660, 709, 730, 760, 920
PkrI GCNGC 1 cut(s) 915
PleI GAGTC 2 cut(s) 278, 454
PpsI GAGTC 2 cut(s) 278, 454
PpuMI RGGWCCY 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 213
Psp6I CCWGG 2 cut(s) 151, 798
PspGI CCWGG 2 cut(s) 151, 798
PspN4I GGNNCC 1 cut(s) 538
PspPI GGNCC 2 cut(s) 87, 213
PspPPI RGGWCCY 1 cut(s) 213
PstI CTGCAG 1 cut(s) 37
RsaI GTAC 1 cut(s) 623
RsaNI GTAC 1 cut(s) 622
RseI CAYNNNNRTG 1 cut(s) 542
SaqAI TTAA 2 cut(s) 819, 879
SatI GCNGC 1 cut(s) 914
Sau3AI GATC 5 cut(s) 37, 113, 247, 517, 891
Sau96I GGNCC 2 cut(s) 87, 213
ScaI AGTACT 1 cut(s) 623
SchI GAGTC 2 cut(s) 278, 454
ScrFI CCNGG 2 cut(s) 153, 800
SduI GDGCHC 1 cut(s) 152
SfaNI GCATC 3 cut(s) 259, 364, 378
SfcI CTRYAG 1 cut(s) 33
SinI GGWCC 2 cut(s) 87, 213
SmiMI CAYNNNNRTG 1 cut(s) 542
Sse9I AATT 4 cut(s) 46, 551, 596, 849
SsiI CCGC 3 cut(s) 299, 318, 684
StyD4I CCNGG 2 cut(s) 151, 798
TaaI ACNGT 1 cut(s) 444
TaqI TCGA 4 cut(s) 182, 372, 825, 894
TaqII GACCGA 1 cut(s) 657
TasI AATT 4 cut(s) 46, 551, 596, 849
TatI WGTACW 1 cut(s) 621
TfiI GAWTC 8 cut(s) 4, 123, 629, 660, 709, 730, 760, 920
Tru1I TTAA 2 cut(s) 819, 879
Tru9I TTAA 2 cut(s) 819, 879
TscAI CASTG 1 cut(s) 97
TseFI GTSAC 1 cut(s) 457
TseI GCWGC 1 cut(s) 913
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 5 cut(s) 119, 162, 435, 707, 897
TspGWI ACGGA 4 cut(s) 75, 758, 827, 870
TspRI CASTG 1 cut(s) 97
VpaK11BI GGWCC 2 cut(s) 87, 213
XapI RAATTY 2 cut(s) 551, 596
XmiI GTMKAC 1 cut(s) 282
ZrmI AGTACT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.