MD09G1253700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012028
Physical Location & Seq
Reverse (-)
32475732 .. 32476412
681 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1253700.v1.1.491

Sequence Viewer

Length: 681 bp
ATGGCAGAGACTAACGGCTCTCTGCAACAAACCCACCTACAAAACCAAGCTTTTCAGTCGCTATTCCAAGCCCTCGACCCCATCGCTCTCATTCTCTCACAGAACCCTAACCCAGATCAGCATCCAGTCCCCCTGAGGTTCGCCACCGAGAGCTACTCCATGGAGAGAGGACCCAGATACAGAGCCTACGCGGAACTCAGAGAATCGCGGCTAAGGAGGAAGTACATGAGCCCAGAGGAGTCCGAAGAACCCGAATCGAAACCGACCCCATTGAAGAAACAAGTCAAATTTCAGACCCATCTGACCGATTCGCGAAAAGGGTCTTCCGTTCTCGCTCAATCGGTGCCTGATTTCTCAGCCGTGTTGCGGAAAGAGAACCGCAAGCCGCCGTCGAGGCTTCCGTCGATGCAGGAGATGACTCCGCCGGCGAAGAGTTGGGCGAAAGCGGCGGACGGGGTTCTGTCGAATTCAAGAGGGAGTAAATCGGCGATCGCGGGAGAGAAGAGGCACAACAATGGTCGAGGCATCATGGGGAGGAAGAGCTACGCGAGTATGGAGGAGTTGAAGGGGTTGTCGTCCGCCGCCGCTAATGCTATTAGCGGCGAAAACAGGGGTGGAAGGAATGCCAACGGCAATGGCAGAGCAATGCCAAAGACTGTTTTGGGGTACAGACAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

25.0

Weight (kDa)

10.18

Isoelectric Point (pI)

53.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67035 AT1G67035 AT5G38300
fragaria_vesca FvH4_6g36230
malus_domestica MD09G1253700.v1.1 MD17G1245500.v1.1
prunus_persica Prupe.3G143600_v2.0.a1
pyrus_communis pycom09g16960 pycom17g24800
rosa_chinensis RchiOBHm_Chr2g0148201
rosa_laevigata RLG00000020326
rosa_multiflora Rmu_sc0001516.1_g000105
rosa_roxburghii Rroxscaffold_2G00099000
rosa_rugosa Rorug02G0404400
rosa_samantha Rh2AG463000 Rh2BG476200 Rh2CG449800 Rh2DG484900
rosa_wichuraiana Rw2G037750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 343
AccII CGCG 5 cut(s) 191, 208, 313, 494, 548
AcsI RAATTY 2 cut(s) 287, 466
AfaI GTAC 2 cut(s) 224, 668
AfiI CCNNNNNNNGG 1 cut(s) 366
AgsI TTSAA 3 cut(s) 274, 471, 565
AluBI AGCT 3 cut(s) 50, 153, 543
AluI AGCT 3 cut(s) 50, 153, 543
Alw26I GTCTC 1 cut(s) 2
ApoI RAATTY 2 cut(s) 287, 466
AsiSI GCGATCGC 1 cut(s) 492
AspS9I GGNCC 1 cut(s) 170
AvaII GGWCC 1 cut(s) 170
AxyI CCTNAGG 1 cut(s) 134
BanI GGYRCC 1 cut(s) 343
BanII GRGCYC 1 cut(s) 233
BbsI GAAGAC 1 cut(s) 315
BccI CCATC 2 cut(s) 89, 306
BceAI ACGGC 4 cut(s) 31, 344, 373, 646
BcoDI GTCTC 1 cut(s) 2
BglI GCCNNNNNGGC 1 cut(s) 394
BisI GCNGC 6 cut(s) 209, 386, 447, 582, 585, 601
BlsI GCNGC 6 cut(s) 210, 387, 448, 583, 586, 602
Bme18I GGWCC 1 cut(s) 170
BmgT120I GGNCC 1 cut(s) 170
BmiI GGNNCC 2 cut(s) 172, 345
BmsI GCATC 3 cut(s) 130, 396, 534
BpiI GAAGAC 1 cut(s) 315
BplI GAGNNNNNCTC 2 cut(s) 140, 172
Bpu10I CCTNAGC 1 cut(s) 212
BsaBI GATNNNNATC 1 cut(s) 120
BsaJI CCNNGG 1 cut(s) 159
Bsc4I CCNNNNNNNGG 1 cut(s) 366
Bse118I RCCGGY 1 cut(s) 424
Bse1I ACTGG 1 cut(s) 125
Bse21I CCTNAGG 1 cut(s) 134
Bse3DI GCAATG 2 cut(s) 640, 651
Bse8I GATNNNNATC 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 159
BseGI GGATG 1 cut(s) 121
BseJI GATNNNNATC 1 cut(s) 120
BseLI CCNNNNNNNGG 1 cut(s) 366
BseMI GCAATG 2 cut(s) 640, 651
BseMII CTCAG 3 cut(s) 125, 211, 369
BseNI ACTGG 1 cut(s) 125
BseRI GAGGAG 2 cut(s) 251, 572
Bsh1236I CGCG 5 cut(s) 191, 208, 313, 494, 548
Bsh1285I CGRYCG 1 cut(s) 492
BshNI GGYRCC 1 cut(s) 343
BsiEI CGRYCG 1 cut(s) 492
BsiSI CCGG 1 cut(s) 425
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 1 cut(s) 366
BsmAI GTCTC 1 cut(s) 2
BsmFI GGGAC 1 cut(s) 113
BsmI GAATGC 1 cut(s) 628
Bsp1286I GDGCHC 1 cut(s) 233
Bsp143I GATC 2 cut(s) 115, 489
Bsp19I CCATGG 1 cut(s) 159
Bsp68I TCGCGA 1 cut(s) 313
BspCNI CTCAG 3 cut(s) 126, 210, 368
BspFNI CGCG 5 cut(s) 191, 208, 313, 494, 548
BspLI GGNNCC 2 cut(s) 172, 345
BspQI GCTCTTC 1 cut(s) 533
BspT107I GGYRCC 1 cut(s) 343
BsrDI GCAATG 2 cut(s) 640, 651
BsrFI RCCGGY 1 cut(s) 424
BsrI ACTGG 1 cut(s) 125
BssAI RCCGGY 1 cut(s) 424
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 2 cut(s) 115, 489
BssT1I CCWWGG 1 cut(s) 159
Bst4CI ACNGT 2 cut(s) 658, 675
Bst6I CTCTTC 3 cut(s) 425, 497, 533
BstC8I GCNNGC 2 cut(s) 383, 426
BstDEI CTNAG 4 cut(s) 134, 197, 212, 355
BstDSI CCRYGG 1 cut(s) 159
BstF5I GGATG 1 cut(s) 121
BstFNI CGCG 5 cut(s) 191, 208, 313, 494, 548
BstKTI GATC 2 cut(s) 118, 492
BstMAI GTCTC 1 cut(s) 2
BstMBI GATC 2 cut(s) 115, 489
BstMCI CGRYCG 1 cut(s) 492
BstMWI GCNNNNNNNGC 3 cut(s) 394, 446, 590
BstUI CGCG 5 cut(s) 191, 208, 313, 494, 548
BstV2I GAAGAC 1 cut(s) 315
Bsu36I CCTNAGG 1 cut(s) 134
BtgI CCRYGG 1 cut(s) 159
BtgZI GCGATG 1 cut(s) 67
BtsCI GGATG 1 cut(s) 121
BtuMI TCGCGA 1 cut(s) 313
Cac8I GCNNGC 2 cut(s) 383, 426
Cfr10I RCCGGY 1 cut(s) 424
Cfr13I GGNCC 1 cut(s) 170
Csp6I GTAC 2 cut(s) 223, 667
CviAII CATG 3 cut(s) 160, 226, 529
CviQI GTAC 2 cut(s) 223, 667
DdeI CTNAG 4 cut(s) 134, 197, 212, 355
DpnI GATC 2 cut(s) 117, 491
DpnII GATC 2 cut(s) 115, 489
Eam1104I CTCTTC 3 cut(s) 425, 497, 533
EarI CTCTTC 3 cut(s) 425, 497, 533
EciI GGCGGA 3 cut(s) 411, 464, 568
Eco130I CCWWGG 1 cut(s) 159
Eco24I GRGCYC 1 cut(s) 233
Eco47I GGWCC 1 cut(s) 170
Eco81I CCTNAGG 1 cut(s) 134
EcoO109I RGGNCCY 1 cut(s) 170
EcoRI GAATTC 1 cut(s) 466
EcoT14I CCWWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 233
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 3 cut(s) 163, 229, 532
FaiI YATR 4 cut(s) 161, 227, 530, 554
FaqI GGGAC 1 cut(s) 113
FatI CATG 3 cut(s) 159, 225, 528
FauI CCCGC 1 cut(s) 487
Fnu4HI GCNGC 6 cut(s) 209, 386, 447, 582, 585, 601
FokI GGATG 1 cut(s) 108
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 6 cut(s) 209, 386, 447, 582, 585, 601
GluI GCNGC 6 cut(s) 209, 386, 447, 582, 585, 601
HapII CCGG 1 cut(s) 425
Hin1II CATG 3 cut(s) 163, 229, 532
HindIII AAGCTT 1 cut(s) 48
HinfI GANTC 5 cut(s) 203, 239, 254, 308, 418
HpaII CCGG 1 cut(s) 425
Hpy188I TCNGA 5 cut(s) 200, 244, 294, 303, 680
Hpy188III TCNNGA 2 cut(s) 312, 471
Hpy99I CGWCG 2 cut(s) 394, 406
HpyAV CCTTC 2 cut(s) 559, 612
HpyCH4III ACNGT 2 cut(s) 658, 675
HpyCH4V TGCA 2 cut(s) 25, 409
HpyF10VI GCNNNNNNNGC 3 cut(s) 394, 446, 590
HpyF3I CTNAG 4 cut(s) 134, 197, 212, 355
Hsp92II CATG 3 cut(s) 163, 229, 532
KroI GCCGGC 1 cut(s) 424
KroNI GCCGGC 1 cut(s) 426
Kzo9I GATC 2 cut(s) 115, 489
LguI GCTCTTC 1 cut(s) 533
LpnPI CCDG 9 cut(s) 126, 138, 146, 187, 246, 360, 395, 438, 595
LweI GCATC 3 cut(s) 130, 396, 534
MalI GATC 2 cut(s) 117, 491
MboI GATC 2 cut(s) 115, 489
MboII GAAGA 6 cut(s) 257, 286, 315, 442, 514, 550
MhlI GDGCHC 1 cut(s) 233
MluCI AATT 2 cut(s) 287, 466
MlyI GAGTC 2 cut(s) 248, 412
MreI CGCCGGCG 1 cut(s) 424
MroNI GCCGGC 1 cut(s) 424
MslI CAYNNNNRTG 1 cut(s) 513
MspI CCGG 1 cut(s) 425
Mva1269I GAATGC 1 cut(s) 628
MvnI CGCG 5 cut(s) 191, 208, 313, 494, 548
MwoI GCNNNNNNNGC 3 cut(s) 394, 446, 590
NaeI GCCGGC 1 cut(s) 426
NcoI CCATGG 1 cut(s) 159
NdeII GATC 2 cut(s) 115, 489
NgoMIV GCCGGC 1 cut(s) 424
NlaIII CATG 3 cut(s) 163, 229, 532
NlaIV GGNNCC 2 cut(s) 172, 345
NruI TCGCGA 1 cut(s) 313
PciSI GCTCTTC 1 cut(s) 533
PcsI WCGNNNNNNNCGW 1 cut(s) 398
PctI GAATGC 1 cut(s) 628
PdiI GCCGGC 1 cut(s) 426
PfeI GAWTC 3 cut(s) 203, 254, 308
PkrI GCNGC 6 cut(s) 210, 387, 448, 583, 586, 602
Ple19I CGATCG 1 cut(s) 492
PleI GAGTC 2 cut(s) 247, 412
PpsI GAGTC 2 cut(s) 247, 412
PpuMI RGGWCCY 1 cut(s) 170
Psp5II RGGWCCY 1 cut(s) 170
PspN4I GGNNCC 2 cut(s) 172, 345
PspPI GGNCC 1 cut(s) 170
PspPPI RGGWCCY 1 cut(s) 170
PsrI GAACNNNNNNTAC 1 cut(s) 659
PvuI CGATCG 1 cut(s) 492
RgaI GCGATCGC 1 cut(s) 492
RruI TCGCGA 1 cut(s) 313
RsaI GTAC 2 cut(s) 224, 668
RsaNI GTAC 2 cut(s) 223, 667
RseI CAYNNNNRTG 1 cut(s) 513
SapI GCTCTTC 1 cut(s) 533
SatI GCNGC 6 cut(s) 209, 386, 447, 582, 585, 601
Sau3AI GATC 2 cut(s) 115, 489
Sau96I GGNCC 1 cut(s) 170
SchI GAGTC 2 cut(s) 248, 412
SduI GDGCHC 1 cut(s) 233
SetI ASST 5 cut(s) 39, 52, 140, 155, 545
SfaAI GCGATCGC 1 cut(s) 492
SfaNI GCATC 3 cut(s) 130, 396, 534
SgfI GCGATCGC 1 cut(s) 492
SgrAI CRCCGGYG 1 cut(s) 424
SinI GGWCC 1 cut(s) 170
SmiMI CAYNNNNRTG 1 cut(s) 513
Sse9I AATT 2 cut(s) 287, 466
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 2 cut(s) 658, 675
TaqI TCGA 6 cut(s) 75, 257, 392, 404, 464, 520
TaqII GACCGA 1 cut(s) 320
TasI AATT 2 cut(s) 287, 466
TatI WGTACW 1 cut(s) 222
TauI GCSGC 6 cut(s) 211, 388, 449, 584, 587, 603
TfiI GAWTC 3 cut(s) 203, 254, 308
TspGWI ACGGA 2 cut(s) 316, 390
VpaK11BI GGWCC 1 cut(s) 170
XapI RAATTY 2 cut(s) 287, 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.