pycom09g16960

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0012028
Physical Location & Seq
Reverse (-)
17390834 .. 17391517
684 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g16960.1

Sequence Viewer

Length: 684 bp
ATGGCAGAGACTAACGGCTCTCTGCAACAAACCCACCTACAAAACCCAGCTTTTCAGTCGCTATTCCAAGCCCTCGACCCCATCGCTCTCATTCTCTCACAGAACCCTAACCCAGATCAGCAGCATCCAGTCCCCCTGAGGTTCGTCACCGAGAGCTATTCCATGGAGAGAGGACCCAGATACAGAGCCTACGCGGAAATCAGAGAATCGCGGCTAAGGAGGAAGTACATGAGCCCAGAGGAGTCCGAAGAACCCGAATCGAAACCGACCCCATTGAAGAAACAAGTCAAATTTCAGACCCATCTGACCGATTCTCGAAAAGGGTCTTCCGTTCTCGCTCAATCGGTGCCTGATTTCTCAGCCGTGTTGCGGAAAGAGAACCGCAAGCCGCCGTCGAGGCTTCCGTCGATGCAGGAGATGACTCCGCCGGCAAAATGTTGGGCGAAAGCGGCGGACGGGGTTCTGTCGAATTCAAGAGGGAGTAAATCGGCGATCGCGGGAGAGAAGAGGTACAACAATGGTGGAGGCGTCATGGGGAGGAAGAGCTACGCGAGTATGGAGGAGTTGAAGGGGCTGTCGTCCGCCGCCGCTAATGCTATTAGCGGCGAAAACAGGGGCGGAAGGAATGCCAACGGCAATGGCAGAGCAATGCCAAAGACTGTTTTGGGGTACAGACAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

228

Amino Acids

25.05

Weight (kDa)

9.99

Isoelectric Point (pI)

55.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67035 AT1G67035 AT5G38300
fragaria_vesca FvH4_6g36230
malus_domestica MD09G1253700.v1.1 MD17G1245500.v1.1
prunus_persica Prupe.3G143600_v2.0.a1
pyrus_communis pycom09g16960 pycom17g24800
rosa_chinensis RchiOBHm_Chr2g0148201
rosa_laevigata RLG00000020326
rosa_multiflora Rmu_sc0001516.1_g000105
rosa_roxburghii Rroxscaffold_2G00099000
rosa_rugosa Rorug02G0404400
rosa_samantha Rh2AG463000 Rh2BG476200 Rh2CG449800 Rh2DG484900
rosa_wichuraiana Rw2G037750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 346
AccII CGCG 4 cut(s) 194, 211, 497, 551
AcsI RAATTY 2 cut(s) 290, 469
AcyI GRCGYC 1 cut(s) 528
AfaI GTAC 3 cut(s) 227, 512, 671
AfiI CCNNNNNNNGG 1 cut(s) 369
AgsI TTSAA 3 cut(s) 277, 474, 568
AluBI AGCT 3 cut(s) 50, 156, 546
AluI AGCT 3 cut(s) 50, 156, 546
Alw26I GTCTC 1 cut(s) 2
ApeKI GCWGC 1 cut(s) 121
ApoI RAATTY 2 cut(s) 290, 469
AsiSI GCGATCGC 1 cut(s) 495
AspS9I GGNCC 1 cut(s) 173
AsuHPI GGTGA 1 cut(s) 139
AvaII GGWCC 1 cut(s) 173
AxyI CCTNAGG 1 cut(s) 137
BanI GGYRCC 1 cut(s) 346
BanII GRGCYC 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 318
BbvI GCAGC 1 cut(s) 133
BccI CCATC 2 cut(s) 89, 309
BceAI ACGGC 4 cut(s) 31, 347, 376, 649
BcoDI GTCTC 1 cut(s) 2
BglI GCCNNNNNGGC 1 cut(s) 397
BisI GCNGC 7 cut(s) 122, 212, 389, 450, 585, 588, 604
BlsI GCNGC 7 cut(s) 123, 213, 390, 451, 586, 589, 605
Bme18I GGWCC 1 cut(s) 173
BmgT120I GGNCC 1 cut(s) 173
BmiI GGNNCC 2 cut(s) 175, 348
BmsI GCATC 2 cut(s) 133, 399
BpiI GAAGAC 1 cut(s) 318
Bpu10I CCTNAGC 1 cut(s) 215
BsaHI GRCGYC 1 cut(s) 528
BsaJI CCNNGG 1 cut(s) 162
Bsc4I CCNNNNNNNGG 1 cut(s) 369
Bse118I RCCGGY 1 cut(s) 427
Bse1I ACTGG 1 cut(s) 128
Bse21I CCTNAGG 1 cut(s) 137
Bse3DI GCAATG 2 cut(s) 643, 654
BseDI CCNNGG 1 cut(s) 162
BseGI GGATG 1 cut(s) 124
BseLI CCNNNNNNNGG 1 cut(s) 369
BseMI GCAATG 2 cut(s) 643, 654
BseMII CTCAG 2 cut(s) 128, 372
BseNI ACTGG 1 cut(s) 128
BseRI GAGGAG 2 cut(s) 254, 575
BseXI GCAGC 1 cut(s) 133
BseYI CCCAGC 1 cut(s) 46
Bsh1236I CGCG 4 cut(s) 194, 211, 497, 551
Bsh1285I CGRYCG 1 cut(s) 495
BshNI GGYRCC 1 cut(s) 346
BsiEI CGRYCG 1 cut(s) 495
BsiSI CCGG 1 cut(s) 428
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 1 cut(s) 369
BsmAI GTCTC 1 cut(s) 2
BsmFI GGGAC 1 cut(s) 116
BsmI GAATGC 1 cut(s) 631
Bsp1286I GDGCHC 1 cut(s) 236
Bsp143I GATC 2 cut(s) 115, 492
Bsp19I CCATGG 1 cut(s) 162
BspCNI CTCAG 2 cut(s) 129, 371
BspFNI CGCG 4 cut(s) 194, 211, 497, 551
BspLI GGNNCC 2 cut(s) 175, 348
BspQI GCTCTTC 1 cut(s) 536
BspT107I GGYRCC 1 cut(s) 346
BsrDI GCAATG 2 cut(s) 643, 654
BsrFI RCCGGY 1 cut(s) 427
BsrI ACTGG 1 cut(s) 128
BssAI RCCGGY 1 cut(s) 427
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 2 cut(s) 115, 492
BssNI GRCGYC 1 cut(s) 528
BssT1I CCWWGG 1 cut(s) 162
Bst4CI ACNGT 2 cut(s) 661, 678
Bst6I CTCTTC 2 cut(s) 500, 536
BstACI GRCGYC 1 cut(s) 528
BstC8I GCNNGC 2 cut(s) 386, 429
BstDEI CTNAG 3 cut(s) 137, 215, 358
BstDSI CCRYGG 1 cut(s) 162
BstF5I GGATG 1 cut(s) 124
BstFNI CGCG 4 cut(s) 194, 211, 497, 551
BstKTI GATC 2 cut(s) 118, 495
BstMAI GTCTC 1 cut(s) 2
BstMBI GATC 2 cut(s) 115, 492
BstMCI CGRYCG 1 cut(s) 495
BstMWI GCNNNNNNNGC 3 cut(s) 397, 449, 593
BstUI CGCG 4 cut(s) 194, 211, 497, 551
BstV1I GCAGC 1 cut(s) 133
BstV2I GAAGAC 1 cut(s) 318
Bsu36I CCTNAGG 1 cut(s) 137
BtgI CCRYGG 1 cut(s) 162
BtgZI GCGATG 1 cut(s) 67
BtsCI GGATG 1 cut(s) 124
Cac8I GCNNGC 2 cut(s) 386, 429
Cfr10I RCCGGY 1 cut(s) 427
Cfr13I GGNCC 1 cut(s) 173
CseI GACGC 1 cut(s) 517
Csp6I GTAC 3 cut(s) 226, 511, 670
CspCI CAANNNNNGTGG 2 cut(s) 502, 537
CviAII CATG 3 cut(s) 163, 229, 532
CviQI GTAC 3 cut(s) 226, 511, 670
DdeI CTNAG 3 cut(s) 137, 215, 358
DpnI GATC 2 cut(s) 117, 494
DpnII GATC 2 cut(s) 115, 492
Eam1104I CTCTTC 2 cut(s) 500, 536
EarI CTCTTC 2 cut(s) 500, 536
EciI GGCGGA 4 cut(s) 414, 467, 571, 633
Eco130I CCWWGG 1 cut(s) 162
Eco24I GRGCYC 1 cut(s) 236
Eco47I GGWCC 1 cut(s) 173
Eco81I CCTNAGG 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 173
EcoRI GAATTC 1 cut(s) 469
EcoT14I CCWWGG 1 cut(s) 162
EcoT38I GRGCYC 1 cut(s) 236
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 3 cut(s) 166, 232, 535
FaiI YATR 4 cut(s) 164, 230, 533, 557
FaqI GGGAC 1 cut(s) 116
FatI CATG 3 cut(s) 162, 228, 531
FauI CCCGC 1 cut(s) 490
Fnu4HI GCNGC 7 cut(s) 122, 212, 389, 450, 585, 588, 604
FokI GGATG 1 cut(s) 111
FriOI GRGCYC 1 cut(s) 236
Fsp4HI GCNGC 7 cut(s) 122, 212, 389, 450, 585, 588, 604
GluI GCNGC 7 cut(s) 122, 212, 389, 450, 585, 588, 604
GsaI CCCAGC 1 cut(s) 50
HapII CCGG 1 cut(s) 428
HgaI GACGC 1 cut(s) 517
Hin1I GRCGYC 1 cut(s) 528
Hin1II CATG 3 cut(s) 166, 232, 535
HinfI GANTC 5 cut(s) 206, 242, 257, 311, 421
HpaII CCGG 1 cut(s) 428
HphI GGTGA 1 cut(s) 139
Hpy188I TCNGA 5 cut(s) 203, 247, 297, 306, 683
Hpy188III TCNNGA 2 cut(s) 315, 474
Hpy99I CGWCG 2 cut(s) 397, 409
HpyAV CCTTC 2 cut(s) 562, 615
HpyCH4III ACNGT 2 cut(s) 661, 678
HpyCH4V TGCA 2 cut(s) 25, 412
HpyF10VI GCNNNNNNNGC 3 cut(s) 397, 449, 593
HpyF3I CTNAG 3 cut(s) 137, 215, 358
Hsp92I GRCGYC 1 cut(s) 528
Hsp92II CATG 3 cut(s) 166, 232, 535
KroI GCCGGC 1 cut(s) 427
KroNI GCCGGC 1 cut(s) 429
Kzo9I GATC 2 cut(s) 115, 492
LguI GCTCTTC 1 cut(s) 536
Lsp1109I GCAGC 1 cut(s) 133
LweI GCATC 2 cut(s) 133, 399
MaeIII GTNAC 1 cut(s) 145
MalI GATC 2 cut(s) 117, 494
MboI GATC 2 cut(s) 115, 492
MboII GAAGA 5 cut(s) 260, 289, 318, 517, 553
MhlI GDGCHC 1 cut(s) 236
MluCI AATT 2 cut(s) 290, 469
MlyI GAGTC 2 cut(s) 251, 415
MroNI GCCGGC 1 cut(s) 427
MspI CCGG 1 cut(s) 428
Mva1269I GAATGC 1 cut(s) 631
MvnI CGCG 4 cut(s) 194, 211, 497, 551
MwoI GCNNNNNNNGC 3 cut(s) 397, 449, 593
NaeI GCCGGC 1 cut(s) 429
NcoI CCATGG 1 cut(s) 162
NdeII GATC 2 cut(s) 115, 492
NgoMIV GCCGGC 1 cut(s) 427
NlaIII CATG 3 cut(s) 166, 232, 535
NlaIV GGNNCC 2 cut(s) 175, 348
NmuCI GTSAC 1 cut(s) 145
PciSI GCTCTTC 1 cut(s) 536
PcsI WCGNNNNNNNCGW 1 cut(s) 401
PctI GAATGC 1 cut(s) 631
PdiI GCCGGC 1 cut(s) 429
PfeI GAWTC 3 cut(s) 206, 257, 311
PkrI GCNGC 7 cut(s) 123, 213, 390, 451, 586, 589, 605
Ple19I CGATCG 1 cut(s) 495
PleI GAGTC 2 cut(s) 250, 415
PpsI GAGTC 2 cut(s) 250, 415
PpuMI RGGWCCY 1 cut(s) 173
Psp5II RGGWCCY 1 cut(s) 173
PspFI CCCAGC 1 cut(s) 46
PspN4I GGNNCC 2 cut(s) 175, 348
PspPI GGNCC 1 cut(s) 173
PspPPI RGGWCCY 1 cut(s) 173
PsrI GAACNNNNNNTAC 1 cut(s) 662
PvuI CGATCG 1 cut(s) 495
RgaI GCGATCGC 1 cut(s) 495
RsaI GTAC 3 cut(s) 227, 512, 671
RsaNI GTAC 3 cut(s) 226, 511, 670
SapI GCTCTTC 1 cut(s) 536
SatI GCNGC 7 cut(s) 122, 212, 389, 450, 585, 588, 604
Sau3AI GATC 2 cut(s) 115, 492
Sau96I GGNCC 1 cut(s) 173
SchI GAGTC 2 cut(s) 251, 415
SduI GDGCHC 1 cut(s) 236
SetI ASST 6 cut(s) 39, 52, 143, 158, 512, 548
SfaAI GCGATCGC 1 cut(s) 495
SfaNI GCATC 2 cut(s) 133, 399
SgfI GCGATCGC 1 cut(s) 495
SinI GGWCC 1 cut(s) 173
Sse9I AATT 2 cut(s) 290, 469
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 2 cut(s) 661, 678
TaqI TCGA 6 cut(s) 75, 260, 316, 395, 407, 467
TaqII GACCGA 1 cut(s) 323
TasI AATT 2 cut(s) 290, 469
TatI WGTACW 1 cut(s) 225
TauI GCSGC 6 cut(s) 214, 391, 452, 587, 590, 606
TfiI GAWTC 3 cut(s) 206, 257, 311
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 1 cut(s) 121
Tsp45I GTSAC 1 cut(s) 145
TspGWI ACGGA 2 cut(s) 319, 393
VpaK11BI GGWCC 1 cut(s) 173
XapI RAATTY 2 cut(s) 290, 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.