MD09G1285400.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013220
Physical Location & Seq
Forward (+)
36364455 .. 36366937
2483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1285400.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGGATGTGAAGAAGGATGTTGTGAAGAGGGAGGACAACAATATGAATGGAGGAGGAGGAGGATGCGGATTTTCCACATCACCATTTGCAAGCATGTTTGATTTCTGTGAAGGAGAAAAGAGTTCATCTTTAGGGTTTATGGAGCTTTTGGGGGCTGGGGTTGGACAGGACTATTGTCCCACTTCCCTGTTTGATTATTTGCCGCAAACACCATCTACAGTCCTGCCTTCATTAGTTTCCACAATGGGAGGAAAAGACCAGTTCTCTGCTGACTACTCCTCTTTGAATCAGCAGCCTGCAACACCAAACTCTTCGTCGGTTTCGTCGGAATCAAGCGAGGCAGTGAACGAAGAACATACTGATCATAAAGCTGCCGCAGACCAAGAGGAAAATAATGAAGAAGAAGAACATGATGAGCAAAAAAATACCAAGAAAGAGTTGAAAGCCAAGAAGGCGAGTCAGAAGAGGCAGAGAGAGCCCAGATTTGCATTCATGACAAAGAGCGAAGTTGATCATCTGGAAGATGGCTACAGATGGAGAAAGTACGGCCAGAAAGCTGTCAAAAATAGCCCCTTTCCCAGGAGTTACTATCGGTGCACAAGTGCATCATGTAACGTGAAGAAACGAGTGGAGCGATCTTTCGACGATCCGAGCATTGTTGTGACTACTTATGAAGGCCAACACACTCATCCGAGCCCACTCTTGCCTCGCCCAACTCTCACTTCAGCTTCTTCTGTTGCTTCTGCTTCTCCTTTTGCACCTAATAATTTTGCCATGCCATTAATCCCCACAAGAACCCTATTTCCTCATCACTATCAACAAGTAGAGCCGTTTGCAGATAACTCCTCTCCGCCTTCATCAGCACCTTTTAATTTTGGTAATTATCATGTGAACGGCTCTTCGTCGTCTCTAACAAATGCTACTAGTACTACCACCGGCCTTGTTCATCCCGAAAGGCGGTTTTGCTCTCCGGCAACCGGTTCTGCTTTGTTAGCTGACCATGGCCTTCTTCAAGACATAGTCCCCTCTCATATGCTTAAGCAAGAGCAGTAG

Protein Analysis

351

Amino Acids

38.39

Weight (kDa)

5.85

Isoelectric Point (pI)

61.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 174 - 231 2.8e-28 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013220)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62340
fragaria_vesca FvH4_1g26980
malus_domestica MD09G1285400.v1.1 MD17G1278100.v1.1
prunus_persica Prupe.3G008600_v2.0.a1 Prupe.3G008600_v2.0.a1 Prupe.3G008600_v2.0.a1
pyrus_communis pycom17g27580
rosa_chinensis RchiOBHm_Chr3g0487201
rosa_laevigata RLG00000023023
rosa_multiflora Rmu_ssc0000366.1_g000027
rosa_roxburghii Rroxscaffold_6G00395030
rosa_rugosa Rorug03G0232300
rosa_samantha Rh3AG280200 Rh3BG316200 Rh3CG314300 Rh3DG311600
rosa_wichuraiana Rw3G024820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 66, 203, 375, 851, 958
AclWI GGATC 1 cut(s) 641
AcoI YGGCCR 1 cut(s) 547
AcuI CTGAAG 1 cut(s) 708
AfaI GTAC 2 cut(s) 545, 928
AfiI CCNNNNNNNGG 3 cut(s) 579, 957, 977
AflII CTTAAG 1 cut(s) 1037
AgeI ACCGGT 1 cut(s) 977
AgsI TTSAA 3 cut(s) 286, 442, 1013
AhlI ACTAGT 1 cut(s) 923
AjnI CCWGG 1 cut(s) 578
AjuI GAANNNNNNNTTGG 2 cut(s) 706, 738
AloI GAACNNNNNNTCC 2 cut(s) 787, 819
AluBI AGCT 5 cut(s) 145, 371, 557, 728, 995
AluI AGCT 5 cut(s) 145, 371, 557, 728, 995
Alw21I GWGCWC 1 cut(s) 599
Alw26I GTCTC 1 cut(s) 912
Alw44I GTGCAC 1 cut(s) 595
AlwI GGATC 1 cut(s) 641
AoxI GGCC 4 cut(s) 547, 676, 937, 1003
ApaLI GTGCAC 1 cut(s) 595
ApeKI GCWGC 2 cut(s) 292, 371
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
AseI ATTAAT 1 cut(s) 782
AsiGI ACCGGT 1 cut(s) 977
AsuHPI GGTGA 1 cut(s) 72
BaeGI GKGCMC 1 cut(s) 599
BanII GRGCYC 2 cut(s) 480, 698
Bbv12I GWGCWC 1 cut(s) 599
BbvI GCAGC 2 cut(s) 304, 358
BccI CCATC 3 cut(s) 220, 518, 528
BceAI ACGGC 3 cut(s) 562, 814, 910
BciT130I CCWGG 1 cut(s) 580
BclI TGATCA 2 cut(s) 361, 511
BcoDI GTCTC 1 cut(s) 912
BcuI ACTAGT 1 cut(s) 923
BfaI CTAG 1 cut(s) 924
BfmI CTRYAG 2 cut(s) 216, 529
BfrI CTTAAG 1 cut(s) 1037
BglI GCCNNNNNGGC 1 cut(s) 452
BisI GCNGC 4 cut(s) 203, 293, 372, 375
BlsI GCNGC 4 cut(s) 204, 294, 373, 376
BmcAI AGTACT 1 cut(s) 928
Bme1390I CCNGG 1 cut(s) 580
BmrFI CCNGG 1 cut(s) 580
BmsI GCATC 2 cut(s) 53, 614
BoxI GACNNNNGTC 1 cut(s) 174
BsaJI CCNNGG 2 cut(s) 578, 1000
BsaWI WCCGGW 1 cut(s) 977
Bsc4I CCNNNNNNNGG 3 cut(s) 579, 957, 977
Bse118I RCCGGY 2 cut(s) 935, 977
Bse1I ACTGG 1 cut(s) 259
BseBI CCWGG 1 cut(s) 580
BseDI CCNNGG 2 cut(s) 578, 1000
BseGI GGATG 5 cut(s) 10, 22, 68, 688, 946
BseLI CCNNNNNNNGG 3 cut(s) 579, 957, 977
BseNI ACTGG 1 cut(s) 259
BseRI GAGGAG 5 cut(s) 66, 69, 72, 268, 835
BseSI GKGCMC 1 cut(s) 599
BseXI GCAGC 2 cut(s) 304, 358
BseYI CCCAGC 1 cut(s) 155
BshFI GGCC 4 cut(s) 549, 678, 939, 1005
BshTI ACCGGT 1 cut(s) 977
BsiHKAI GWGCWC 1 cut(s) 599
BsiSI CCGG 3 cut(s) 936, 971, 978
BslFI GGGAC 2 cut(s) 162, 1007
BslI CCNNNNNNNGG 3 cut(s) 579, 957, 977
BsmAI GTCTC 1 cut(s) 912
BsmBI CGTCTC 1 cut(s) 912
BsmFI GGGAC 2 cut(s) 162, 1007
BsmI GAATGC 1 cut(s) 488
BsnI GGCC 4 cut(s) 549, 678, 939, 1005
Bsp1286I GDGCHC 3 cut(s) 480, 599, 698
Bsp143I GATC 4 cut(s) 361, 511, 635, 646
Bsp19I CCATGG 1 cut(s) 1000
BspACI CCGC 5 cut(s) 66, 203, 375, 851, 958
BspANI GGCC 4 cut(s) 549, 678, 939, 1005
BspHI TCATGA 1 cut(s) 492
BspPI GGATC 1 cut(s) 641
BspQI GCTCTTC 1 cut(s) 904
BspTI CTTAAG 1 cut(s) 1037
BsrFI RCCGGY 2 cut(s) 935, 977
BsrI ACTGG 1 cut(s) 259
BssAI RCCGGY 2 cut(s) 935, 977
BssECI CCNNGG 2 cut(s) 578, 1000
BssMI GATC 4 cut(s) 361, 511, 635, 646
BssT1I CCWWGG 1 cut(s) 1000
Bst2UI CCWGG 1 cut(s) 580
Bst4CI ACNGT 1 cut(s) 220
Bst6I CTCTTC 4 cut(s) 20, 316, 458, 904
BstAFI CTTAAG 1 cut(s) 1037
BstC8I GCNNGC 2 cut(s) 91, 297
BstDSI CCRYGG 1 cut(s) 1000
BstENI CCTNNNNNAGG 1 cut(s) 577
BstF5I GGATG 5 cut(s) 10, 22, 68, 688, 946
BstKTI GATC 4 cut(s) 364, 514, 638, 649
BstMAI GTCTC 1 cut(s) 912
BstMBI GATC 4 cut(s) 361, 511, 635, 646
BstMWI GCNNNNNNNGC 3 cut(s) 452, 475, 992
BstNI CCWGG 1 cut(s) 580
BstNSI RCATGY 1 cut(s) 97
BstPAI GACNNNNGTC 1 cut(s) 174
BstSCI CCNGG 1 cut(s) 578
BstSFI CTRYAG 2 cut(s) 216, 529
BstSLI GKGCMC 1 cut(s) 599
BstV1I GCAGC 2 cut(s) 304, 358
BsuRI GGCC 4 cut(s) 549, 678, 939, 1005
BtgI CCRYGG 1 cut(s) 1000
BtsCI GGATG 5 cut(s) 10, 22, 68, 688, 946
BtsI GCAGTG 1 cut(s) 348
BtsIMutI CAGTG 1 cut(s) 348
Cac8I GCNNGC 2 cut(s) 91, 297
CciI TCATGA 1 cut(s) 492
Cfr10I RCCGGY 2 cut(s) 935, 977
Csp6I GTAC 2 cut(s) 544, 927
CspAI ACCGGT 1 cut(s) 977
CspCI CAANNNNNGTGG 2 cut(s) 922, 957
CviAII CATG 7 cut(s) 94, 410, 493, 609, 775, 887, 1001
CviQI GTAC 2 cut(s) 544, 927
DpnI GATC 4 cut(s) 363, 513, 637, 648
DpnII GATC 4 cut(s) 361, 511, 635, 646
EaeI YGGCCR 1 cut(s) 547
Eam1104I CTCTTC 4 cut(s) 20, 316, 458, 904
EarI CTCTTC 4 cut(s) 20, 316, 458, 904
EciI GGCGGA 1 cut(s) 840
Eco130I CCWWGG 1 cut(s) 1000
Eco24I GRGCYC 2 cut(s) 480, 698
Eco57I CTGAAG 1 cut(s) 708
EcoNI CCTNNNNNAGG 1 cut(s) 577
EcoRII CCWGG 1 cut(s) 578
EcoT14I CCWWGG 1 cut(s) 1000
EcoT38I GRGCYC 2 cut(s) 480, 698
ErhI CCWWGG 1 cut(s) 1000
Esp3I CGTCTC 1 cut(s) 912
FaeI CATG 7 cut(s) 97, 413, 496, 612, 778, 890, 1004
FaqI GGGAC 2 cut(s) 162, 1007
FatI CATG 7 cut(s) 93, 409, 492, 608, 774, 886, 1000
FauNDI CATATG 1 cut(s) 1032
FbaI TGATCA 2 cut(s) 361, 511
Fnu4HI GCNGC 4 cut(s) 203, 293, 372, 375
FokI GGATG 5 cut(s) 17, 29, 75, 675, 933
FriOI GRGCYC 2 cut(s) 480, 698
Fsp4HI GCNGC 4 cut(s) 203, 293, 372, 375
FspBI CTAG 1 cut(s) 924
GluI GCNGC 4 cut(s) 203, 293, 372, 375
GsaI CCCAGC 1 cut(s) 159
HaeIII GGCC 4 cut(s) 549, 678, 939, 1005
HapII CCGG 3 cut(s) 936, 971, 978
Hin1II CATG 7 cut(s) 97, 413, 496, 612, 778, 890, 1004
HinfI GANTC 3 cut(s) 286, 329, 457
HpaII CCGG 3 cut(s) 936, 971, 978
HphI GGTGA 1 cut(s) 72
Hpy166II GTNNAC 3 cut(s) 346, 597, 892
Hpy188I TCNGA 4 cut(s) 328, 462, 651, 693
Hpy188III TCNNGA 4 cut(s) 493, 518, 950, 1013
Hpy8I GTNNAC 3 cut(s) 346, 597, 892
Hpy99I CGWCG 4 cut(s) 319, 328, 647, 907
HpyAV CCTTC 7 cut(s) 7, 104, 237, 445, 668, 864, 1016
HpyCH4III ACNGT 1 cut(s) 220
HpyCH4IV ACGT 1 cut(s) 615
HpyCH4V TGCA 7 cut(s) 89, 299, 488, 597, 605, 758, 836
HpyF10VI GCNNNNNNNGC 3 cut(s) 452, 475, 992
HpySE526I ACGT 1 cut(s) 615
Hsp92II CATG 7 cut(s) 97, 413, 496, 612, 778, 890, 1004
Ksp22I TGATCA 2 cut(s) 361, 511
Kzo9I GATC 4 cut(s) 361, 511, 635, 646
LguI GCTCTTC 1 cut(s) 904
LmnI GCTCC 2 cut(s) 142, 631
Lsp1109I GCAGC 2 cut(s) 304, 358
LweI GCATC 2 cut(s) 53, 614
MaeI CTAG 1 cut(s) 924
MaeII ACGT 1 cut(s) 615
MaeIII GTNAC 3 cut(s) 584, 611, 661
MalI GATC 4 cut(s) 363, 513, 637, 648
MboI GATC 4 cut(s) 361, 511, 635, 646
MhlI GDGCHC 3 cut(s) 480, 599, 698
MluCI AATT 3 cut(s) 766, 871, 880
MlyI GAGTC 1 cut(s) 466
MmeI TCCRAC 2 cut(s) 142, 306
MseI TTAA 3 cut(s) 782, 870, 1038
MslI CAYNNNNRTG 1 cut(s) 659
MspCI CTTAAG 1 cut(s) 1037
MspI CCGG 3 cut(s) 936, 971, 978
MspR9I CCNGG 1 cut(s) 580
Mva1269I GAATGC 1 cut(s) 488
MvaI CCWGG 1 cut(s) 580
MwoI GCNNNNNNNGC 3 cut(s) 452, 475, 992
NcoI CCATGG 1 cut(s) 1000
NdeI CATATG 1 cut(s) 1032
NdeII GATC 4 cut(s) 361, 511, 635, 646
NlaIII CATG 7 cut(s) 97, 413, 496, 612, 778, 890, 1004
NmuCI GTSAC 1 cut(s) 661
NspI RCATGY 1 cut(s) 97
PagI TCATGA 1 cut(s) 492
PciSI GCTCTTC 1 cut(s) 904
PcsI WCGNNNNNNNCGW 2 cut(s) 320, 631
PctI GAATGC 1 cut(s) 488
PfeI GAWTC 2 cut(s) 286, 329
PflFI GACNNNGTC 1 cut(s) 1019
PinAI ACCGGT 1 cut(s) 977
PkrI GCNGC 4 cut(s) 204, 294, 373, 376
PleI GAGTC 1 cut(s) 465
PpsI GAGTC 1 cut(s) 465
PshAI GACNNNNGTC 1 cut(s) 174
PshBI ATTAAT 1 cut(s) 782
Psp6I CCWGG 1 cut(s) 578
PspFI CCCAGC 1 cut(s) 155
PspGI CCWGG 1 cut(s) 578
PsyI GACNNNGTC 1 cut(s) 1019
RsaI GTAC 2 cut(s) 545, 928
RsaNI GTAC 2 cut(s) 544, 927
RseI CAYNNNNRTG 1 cut(s) 659
SapI GCTCTTC 1 cut(s) 904
SaqAI TTAA 3 cut(s) 782, 870, 1038
SatI GCNGC 4 cut(s) 203, 293, 372, 375
Sau3AI GATC 4 cut(s) 361, 511, 635, 646
ScaI AGTACT 1 cut(s) 928
SchI GAGTC 1 cut(s) 466
ScrFI CCNGG 1 cut(s) 580
SduI GDGCHC 3 cut(s) 480, 599, 698
SetI ASST 8 cut(s) 147, 373, 559, 618, 730, 763, 868, 997
SfaNI GCATC 2 cut(s) 53, 614
SfcI CTRYAG 2 cut(s) 216, 529
SmiMI CAYNNNNRTG 1 cut(s) 659
SmlI CTYRAG 1 cut(s) 1037
SmoI CTYRAG 1 cut(s) 1037
SpeI ACTAGT 1 cut(s) 923
Sse9I AATT 3 cut(s) 766, 871, 880
SsiI CCGC 5 cut(s) 66, 203, 375, 851, 958
SspMI CTAG 1 cut(s) 924
StyD4I CCNGG 1 cut(s) 578
StyI CCWWGG 1 cut(s) 1000
TaaI ACNGT 1 cut(s) 220
TaiI ACGT 1 cut(s) 618
TaqI TCGA 1 cut(s) 642
TasI AATT 3 cut(s) 766, 871, 880
TatI WGTACW 1 cut(s) 926
TauI GCSGC 2 cut(s) 205, 377
TfiI GAWTC 2 cut(s) 286, 329
Tru1I TTAA 3 cut(s) 782, 870, 1038
Tru9I TTAA 3 cut(s) 782, 870, 1038
TscAI CASTG 1 cut(s) 348
TseFI GTSAC 1 cut(s) 661
TseI GCWGC 2 cut(s) 292, 371
Tsp45I GTSAC 1 cut(s) 661
TspDTI ATGAA 8 cut(s) 59, 114, 219, 411, 481, 687, 846, 935
TspRI CASTG 1 cut(s) 348
Tth111I GACNNNGTC 1 cut(s) 1019
Vha464I CTTAAG 1 cut(s) 1037
VneI GTGCAC 1 cut(s) 595
VspI ATTAAT 1 cut(s) 782
XagI CCTNNNNNAGG 1 cut(s) 577
XceI RCATGY 1 cut(s) 97
XspI CTAG 1 cut(s) 924
ZrmI AGTACT 1 cut(s) 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.