Rroxscaffold_6G00395030
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0013220
Physical Location & Seq
Forward (+)
15997837 .. 15999340
1504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00395030.1

Sequence Viewer

Length: 948 bp
ATGGAGAAGAAGGATCATTTGATAAAGATGGAGGGCAATACTAGATCCTCATCATTTTCAGAAACTACAGCTGATTATAGCTACCCATTTCCAGGCATGTTTGATTTCTCTCAAGGCGATCAGAAGGACTCGTTAGGGTTTATGGAGCTTTTGGGTATCCAGGACTTTTGTCCTGCTAATTCCCTCTTTGATTTGCCACAGATCGCTACACCACAAGTACTGCCGACTACTTCCATTGATATGGCGAAAGAGTACTCTTTCAATCCTTCAACACCAAACTCCTCATCGATTTCATCCGCTTCAAGTGAAGCTGTGAACGAGGAACCACATACTGATAATAAAGCTTCTGCAGACCAACAAGAAGAAGATGATGGTCATGACCAACCAAAGCCCAAGAAAGGGTTGAAAGCGAATCAGAAGAGGAAGAAGACAACTGAGAAGAAACTAAGAGAGCCGAGATTCGCGTTCATGACAAAGAGTGAGGTTGATCATCTGGAAGATGGGTACAGATGGAGAAAGTACGGCCAAAAAGCCGTCAAAAACAGCCCTTTCCCAAGGAGCTACTTTCGGTGCACCAGTGCCTCATGTAATGTGAAGAAGCGTGTCGAGCGATGCGTCAACGATCCAGGCATCGTTGTGACGACCTATGAAGGCCAACATACTCACCTAAGTCCTCTTATGCTGCCTCCTCCTCCTCCTCCTCCAACTACTGCACCGAATAGCATTATCATGCCAAGAACCCTATTCTCCCAGTATCAACAGCAACAAGGTCTGCGGCCATTTGCGGATAATCAAGGCTGCAATTTGGGTTTCTCGACACCAAATGGTACAAACCGGAGTTTTCTTAACGAAAGGTGGTTTTGCAGCCCGGCAGGAGGTTCAGCTTTGATGACAGACCATGGCCTTCTTCAAGACATAGTCCCCTCCAATATGTTGAAGCAAGGGTAG

Protein Analysis

315

Amino Acids

35.18

Weight (kDa)

7.04

Isoelectric Point (pI)

55.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 166 - 222 5.4e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013220)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62340
fragaria_vesca FvH4_1g26980
malus_domestica MD09G1285400.v1.1 MD17G1278100.v1.1
prunus_persica Prupe.3G008600_v2.0.a1 Prupe.3G008600_v2.0.a1 Prupe.3G008600_v2.0.a1
pyrus_communis pycom17g27580
rosa_chinensis RchiOBHm_Chr3g0487201
rosa_laevigata RLG00000023023
rosa_multiflora Rmu_ssc0000366.1_g000027
rosa_roxburghii Rroxscaffold_6G00395030
rosa_rugosa Rorug03G0232300
rosa_samantha Rh3AG280200 Rh3BG316200 Rh3CG314300 Rh3DG311600
rosa_wichuraiana Rw3G024820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 464
AciI CCGC 3 cut(s) 297, 775, 785
AclWI GGATC 3 cut(s) 21, 39, 617
AcoI YGGCCR 2 cut(s) 523, 776
AfaI GTAC 5 cut(s) 219, 254, 506, 521, 829
AfiI CCNNNNNNNGG 4 cut(s) 92, 398, 399, 875
AgsI TTSAA 6 cut(s) 262, 270, 303, 406, 911, 937
AjnI CCWGG 3 cut(s) 91, 159, 625
AluBI AGCT 7 cut(s) 71, 81, 148, 311, 344, 561, 884
AluI AGCT 7 cut(s) 71, 81, 148, 311, 344, 561, 884
Alw21I GWGCWC 1 cut(s) 575
Alw44I GTGCAC 1 cut(s) 571
AlwI GGATC 3 cut(s) 21, 39, 617
AoxI GGCC 4 cut(s) 523, 652, 776, 901
ApaLI GTGCAC 1 cut(s) 571
ApeKI GCWGC 3 cut(s) 682, 798, 864
AsuC2I CCSGG 1 cut(s) 869
AsuHPI GGTGA 1 cut(s) 656
BaeGI GKGCMC 1 cut(s) 575
BbsI GAAGAC 1 cut(s) 434
Bbv12I GWGCWC 1 cut(s) 575
BbvI GCAGC 3 cut(s) 669, 785, 876
BccI CCATC 4 cut(s) 22, 365, 494, 504
BceAI ACGGC 2 cut(s) 518, 538
BciT130I CCWGG 3 cut(s) 93, 161, 627
BciVI GTATCC 1 cut(s) 167
BclI TGATCA 1 cut(s) 487
BcnI CCSGG 1 cut(s) 869
BfaI CTAG 1 cut(s) 42
BfmI CTRYAG 2 cut(s) 66, 348
BfuI GTATCC 1 cut(s) 167
BisI GCNGC 4 cut(s) 683, 776, 799, 865
BlsI GCNGC 4 cut(s) 684, 777, 800, 866
BmcAI AGTACT 2 cut(s) 219, 254
Bme1390I CCNGG 4 cut(s) 93, 161, 627, 869
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 4 cut(s) 93, 161, 627, 869
BmrI ACTGGG 1 cut(s) 745
BmsI GCATC 2 cut(s) 602, 639
BmuI ACTGGG 1 cut(s) 745
BoxI GACNNNNGTC 1 cut(s) 168
BpiI GAAGAC 1 cut(s) 434
BpuEI CTTGAG 1 cut(s) 96
BpuMI CCSGG 1 cut(s) 869
Bsa29I ATCGAT 1 cut(s) 287
BsaBI GATNNNNATC 1 cut(s) 49
BsaJI CCNNGG 2 cut(s) 554, 898
BsaWI WCCGGW 1 cut(s) 834
Bsc4I CCNNNNNNNGG 4 cut(s) 92, 398, 399, 875
Bse1I ACTGG 2 cut(s) 576, 751
Bse8I GATNNNNATC 1 cut(s) 49
BseBI CCWGG 3 cut(s) 93, 161, 627
BseCI ATCGAT 1 cut(s) 287
BseDI CCNNGG 2 cut(s) 554, 898
BseGI GGATG 1 cut(s) 293
BseJI GATNNNNATC 1 cut(s) 49
BseLI CCNNNNNNNGG 4 cut(s) 92, 398, 399, 875
BseMII CTCAG 1 cut(s) 426
BseNI ACTGG 2 cut(s) 576, 751
BseRI GAGGAG 6 cut(s) 271, 678, 681, 684, 687, 690
BseSI GKGCMC 1 cut(s) 575
BseXI GCAGC 3 cut(s) 669, 785, 876
BsgI GTGCAG 1 cut(s) 696
Bsh1236I CGCG 1 cut(s) 464
BshFI GGCC 4 cut(s) 525, 654, 778, 903
BshVI ATCGAT 1 cut(s) 287
BsiHKAI GWGCWC 1 cut(s) 575
BsiSI CCGG 2 cut(s) 835, 869
BslFI GGGAC 1 cut(s) 905
BslI CCNNNNNNNGG 4 cut(s) 92, 398, 399, 875
BsmFI GGGAC 1 cut(s) 905
BsnI GGCC 4 cut(s) 525, 654, 778, 903
Bsp1286I GDGCHC 1 cut(s) 575
Bsp143I GATC 6 cut(s) 13, 44, 118, 201, 487, 622
Bsp19I CCATGG 1 cut(s) 898
BspACI CCGC 3 cut(s) 297, 775, 785
BspANI GGCC 4 cut(s) 525, 654, 778, 903
BspCNI CTCAG 1 cut(s) 427
BspDI ATCGAT 1 cut(s) 287
BspFNI CGCG 1 cut(s) 464
BspHI TCATGA 2 cut(s) 376, 468
BspLI GGNNCC 1 cut(s) 324
BspMAI CTGCAG 1 cut(s) 352
BspPI GGATC 3 cut(s) 21, 39, 617
BsrI ACTGG 2 cut(s) 576, 751
BssECI CCNNGG 2 cut(s) 554, 898
BssMI GATC 6 cut(s) 13, 44, 118, 201, 487, 622
BssT1I CCWWGG 2 cut(s) 554, 898
Bst2UI CCWGG 3 cut(s) 93, 161, 627
Bst6I CTCTTC 1 cut(s) 413
BstDEI CTNAG 3 cut(s) 435, 446, 668
BstDSI CCRYGG 1 cut(s) 898
BstF5I GGATG 1 cut(s) 293
BstFNI CGCG 1 cut(s) 464
BstKTI GATC 6 cut(s) 16, 47, 121, 204, 490, 625
BstMBI GATC 6 cut(s) 13, 44, 118, 201, 487, 622
BstMWI GCNNNNNNNGC 1 cut(s) 607
BstNI CCWGG 3 cut(s) 93, 161, 627
BstNSI RCATGY 1 cut(s) 100
BstPAI GACNNNNGTC 1 cut(s) 168
BstSCI CCNGG 4 cut(s) 91, 159, 625, 867
BstSFI CTRYAG 2 cut(s) 66, 348
BstSLI GKGCMC 1 cut(s) 575
BstUI CGCG 1 cut(s) 464
BstV1I GCAGC 3 cut(s) 669, 785, 876
BstV2I GAAGAC 1 cut(s) 434
BstX2I RGATCY 1 cut(s) 44
BstXI CCANNNNNNTGG 1 cut(s) 241
BstYI RGATCY 1 cut(s) 44
Bsu15I ATCGAT 1 cut(s) 287
BsuI GTATCC 1 cut(s) 167
BsuRI GGCC 4 cut(s) 525, 654, 778, 903
BsuTUI ATCGAT 1 cut(s) 287
BtgI CCRYGG 1 cut(s) 898
BtgZI GCGATG 1 cut(s) 625
BtsCI GGATG 1 cut(s) 293
BtsIMutI CAGTG 1 cut(s) 583
CciI TCATGA 2 cut(s) 376, 468
ClaI ATCGAT 1 cut(s) 287
CseI GACGC 1 cut(s) 604
Csp6I GTAC 5 cut(s) 218, 253, 505, 520, 828
CviAII CATG 6 cut(s) 97, 377, 469, 585, 730, 899
CviQI GTAC 5 cut(s) 218, 253, 505, 520, 828
DdeI CTNAG 3 cut(s) 435, 446, 668
DpnI GATC 6 cut(s) 15, 46, 120, 203, 489, 624
DpnII GATC 6 cut(s) 13, 44, 118, 201, 487, 622
EaeI YGGCCR 2 cut(s) 523, 776
Eam1104I CTCTTC 1 cut(s) 413
EarI CTCTTC 1 cut(s) 413
Eco130I CCWWGG 2 cut(s) 554, 898
EcoRII CCWGG 3 cut(s) 91, 159, 625
EcoT14I CCWWGG 2 cut(s) 554, 898
ErhI CCWWGG 2 cut(s) 554, 898
FaeI CATG 6 cut(s) 100, 380, 472, 588, 733, 902
FaqI GGGAC 1 cut(s) 905
FatI CATG 6 cut(s) 96, 376, 468, 584, 729, 898
FbaI TGATCA 1 cut(s) 487
Fnu4HI GCNGC 4 cut(s) 683, 776, 799, 865
FokI GGATG 1 cut(s) 280
Fsp4HI GCNGC 4 cut(s) 683, 776, 799, 865
FspBI CTAG 1 cut(s) 42
GluI GCNGC 4 cut(s) 683, 776, 799, 865
HaeIII GGCC 4 cut(s) 525, 654, 778, 903
HapII CCGG 2 cut(s) 835, 869
HgaI GACGC 1 cut(s) 604
Hin1II CATG 6 cut(s) 100, 380, 472, 588, 733, 902
HincII GTYRAC 1 cut(s) 619
HindII GTYRAC 1 cut(s) 619
HindIII AAGCTT 1 cut(s) 342
HinfI GANTC 3 cut(s) 128, 412, 459
HpaII CCGG 2 cut(s) 835, 869
HphI GGTGA 1 cut(s) 656
Hpy166II GTNNAC 3 cut(s) 316, 573, 619
Hpy188I TCNGA 3 cut(s) 61, 123, 417
Hpy188III TCNNGA 5 cut(s) 377, 469, 494, 814, 911
Hpy8I GTNNAC 3 cut(s) 316, 573, 619
HpyAV CCTTC 5 cut(s) 4, 118, 276, 644, 914
HpyCH4V TGCA 5 cut(s) 350, 573, 713, 801, 864
HpyF10VI GCNNNNNNNGC 1 cut(s) 607
HpyF3I CTNAG 3 cut(s) 435, 446, 668
Hsp92II CATG 6 cut(s) 100, 380, 472, 588, 733, 902
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 6 cut(s) 13, 44, 118, 201, 487, 622
LmnI GCTCC 2 cut(s) 145, 558
Lsp1109I GCAGC 3 cut(s) 669, 785, 876
LweI GCATC 2 cut(s) 602, 639
MaeI CTAG 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 637
MalI GATC 6 cut(s) 15, 46, 120, 203, 489, 624
MboI GATC 6 cut(s) 13, 44, 118, 201, 487, 622
MflI RGATCY 1 cut(s) 44
MhlI GDGCHC 1 cut(s) 575
MluCI AATT 2 cut(s) 178, 802
MlyI GAGTC 1 cut(s) 122
MmeI TCCRAC 1 cut(s) 728
MseI TTAA 1 cut(s) 846
MslI CAYNNNNRTG 3 cut(s) 239, 635, 728
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 2 cut(s) 835, 869
MspR9I CCNGG 4 cut(s) 93, 161, 627, 869
MvaI CCWGG 3 cut(s) 93, 161, 627
MvnI CGCG 1 cut(s) 464
MwoI GCNNNNNNNGC 1 cut(s) 607
NciI CCSGG 1 cut(s) 869
NcoI CCATGG 1 cut(s) 898
NdeII GATC 6 cut(s) 13, 44, 118, 201, 487, 622
NlaIII CATG 6 cut(s) 100, 380, 472, 588, 733, 902
NlaIV GGNNCC 1 cut(s) 324
NmeAIII GCCGAG 1 cut(s) 480
NmuCI GTSAC 1 cut(s) 637
NspI RCATGY 1 cut(s) 100
PagI TCATGA 2 cut(s) 376, 468
PcsI WCGNNNNNNNCGW 1 cut(s) 612
PfeI GAWTC 2 cut(s) 412, 459
PflFI GACNNNGTC 1 cut(s) 917
PfoI TCCNGGA 1 cut(s) 159
PkrI GCNGC 4 cut(s) 684, 777, 800, 866
PleI GAGTC 1 cut(s) 122
PpsI GAGTC 1 cut(s) 122
PshAI GACNNNNGTC 1 cut(s) 168
Psp6I CCWGG 3 cut(s) 91, 159, 625
PspGI CCWGG 3 cut(s) 91, 159, 625
PspN4I GGNNCC 1 cut(s) 324
PstI CTGCAG 1 cut(s) 352
PsuI RGATCY 1 cut(s) 44
PsyI GACNNNGTC 1 cut(s) 917
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 5 cut(s) 219, 254, 506, 521, 829
RsaNI GTAC 5 cut(s) 218, 253, 505, 520, 828
RseI CAYNNNNRTG 3 cut(s) 239, 635, 728
SaqAI TTAA 1 cut(s) 846
SatI GCNGC 4 cut(s) 683, 776, 799, 865
Sau3AI GATC 6 cut(s) 13, 44, 118, 201, 487, 622
ScaI AGTACT 2 cut(s) 219, 254
SchI GAGTC 1 cut(s) 122
ScrFI CCNGG 4 cut(s) 93, 161, 627, 869
SduI GDGCHC 1 cut(s) 575
SfaNI GCATC 2 cut(s) 602, 639
SfcI CTRYAG 2 cut(s) 66, 348
SmiMI CAYNNNNRTG 3 cut(s) 239, 635, 728
SmlI CTYRAG 1 cut(s) 111
SmoI CTYRAG 1 cut(s) 111
Sse9I AATT 2 cut(s) 178, 802
SsiI CCGC 3 cut(s) 297, 775, 785
SspMI CTAG 1 cut(s) 42
StyD4I CCNGG 4 cut(s) 91, 159, 625, 867
StyI CCWWGG 2 cut(s) 554, 898
TaqI TCGA 3 cut(s) 287, 606, 815
TasI AATT 2 cut(s) 178, 802
TatI WGTACW 2 cut(s) 217, 252
TauI GCSGC 1 cut(s) 778
TfiI GAWTC 2 cut(s) 412, 459
Tru1I TTAA 1 cut(s) 846
Tru9I TTAA 1 cut(s) 846
TscAI CASTG 1 cut(s) 583
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 3 cut(s) 682, 798, 864
Tsp45I GTSAC 1 cut(s) 637
TspDTI ATGAA 3 cut(s) 282, 457, 663
TspRI CASTG 1 cut(s) 583
Tth111I GACNNNGTC 1 cut(s) 917
VneI GTGCAC 1 cut(s) 571
XceI RCATGY 1 cut(s) 100
XspI CTAG 1 cut(s) 42
ZrmI AGTACT 2 cut(s) 219, 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.