MD17G1278100.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013220
Physical Location & Seq
Forward (+)
33812003 .. 33814316
2314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1278100.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGAATGTGAAGAAGGATGTGGTGAAGAGGGAGGACAAAATTAATAGCAACAATATGAATGGGGGAGGAGGAGGAGGTGGACTTACCACATCACCATTTGCAGGCATGTTTGATTTCTGTGAAGGAGAAAAGAGTTCATCTTTAGGGTTTATGGAACTTTTGGGGGTTGGGGTTGGACAGGACTGTTGTCCCACTTCCCTGTTTGATTATCTGCCTCAGACACCATCGACAGCCCTGCCTTCATTAGCTTCCACAATGGGAGGAAAAGATCAGTTATCTACTGACTACTCCTCTTTGAATCAGCAGCCTACAACACCAAACTCTTCATCGATATCGTCGGAATCAAGCGAGGCAGTGAACGAAGAACACACTGATCATAAAGCTGCTACAGACCAAGAGAAAAGTAATGAAGAAGAAGAACATCATGAGCAACAAAAGACCAAGAAAGAGTTGAAAGCAAAGAAGACGAGTCAGAAAAGGCCGAGAGAACCCAGATTTGCATTCATGACAAAGAGCGAAGTTGATCATCTGGAAGATGGCTACAGATGGAGAAAGTACGGCCAAAAAGCTGTCAAAAATAGCCCCTTTCCCAGGAGTTACTATCGGTGCACAAGTGCATCATGTAATGTGAAGAAGCGAGTGGAGCGATCTTTCGATGATCCGAGCATTGTTGTAACTACTTATGAAGGCCAACACACTCATCGCAGCCCACTCCTGCCTCGCCCAACTCTCCCTGCGGCTTCTGCTTTTCCAGCTAATAACTTTGAGATGCCATTAATCCCCACAAGAACCCTATTTACTCATGACTATCAACAAGTACAACCATTTGCAGATAACAACTCCTCTTCGCCTTCATCGGCACCAATTAATTTTGGTGATTATTATGTGAATGGCTCTTCATTGTTTACAAATGCTACTAGTACCACGGGCTTTCTTCATCCCGAAAGGCGGTTTTGCTCTCCGGCAACCGGTTCTGCTTTGTTAGCTGACCATGGCCTTCTTCAAGACATAGTCCCCTCTCATATGCTGAAGCAAGAGTAG

Protein Analysis

347

Amino Acids

38.17

Weight (kDa)

6.27

Isoelectric Point (pI)

58.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 178 - 234 6.4e-28 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013220)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62340
fragaria_vesca FvH4_1g26980
malus_domestica MD09G1285400.v1.1 MD17G1278100.v1.1
prunus_persica Prupe.3G008600_v2.0.a1 Prupe.3G008600_v2.0.a1 Prupe.3G008600_v2.0.a1
pyrus_communis pycom17g27580
rosa_chinensis RchiOBHm_Chr3g0487201
rosa_laevigata RLG00000023023
rosa_multiflora Rmu_ssc0000366.1_g000027
rosa_roxburghii Rroxscaffold_6G00395030
rosa_rugosa Rorug03G0232300
rosa_samantha Rh3AG280200 Rh3BG316200 Rh3CG314300 Rh3DG311600
rosa_wichuraiana Rw3G024820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 859
AciI CCGC 2 cut(s) 737, 949
AclWI GGATC 1 cut(s) 653
AcoI YGGCCR 1 cut(s) 559
AfaI GTAC 3 cut(s) 557, 819, 922
AfiI CCNNNNNNNGG 4 cut(s) 101, 591, 948, 968
AgeI ACCGGT 1 cut(s) 968
AgsI TTSAA 3 cut(s) 298, 454, 1004
AhlI ACTAGT 1 cut(s) 917
AjnI CCWGG 1 cut(s) 590
AluBI AGCT 5 cut(s) 248, 383, 569, 755, 986
AluI AGCT 5 cut(s) 248, 383, 569, 755, 986
Alw21I GWGCWC 1 cut(s) 611
Alw44I GTGCAC 1 cut(s) 607
AlwI GGATC 1 cut(s) 653
AoxI GGCC 4 cut(s) 479, 559, 688, 994
ApaLI GTGCAC 1 cut(s) 607
ApeKI GCWGC 3 cut(s) 304, 383, 705
AseI ATTAAT 3 cut(s) 42, 776, 867
AsiGI ACCGGT 1 cut(s) 968
AsuHPI GGTGA 3 cut(s) 34, 84, 887
BaeGI GKGCMC 1 cut(s) 611
BanI GGYRCC 1 cut(s) 859
BbsI GAAGAC 1 cut(s) 470
Bbv12I GWGCWC 1 cut(s) 611
BbvI GCAGC 3 cut(s) 316, 370, 717
BccI CCATC 3 cut(s) 232, 530, 540
BceAI ACGGC 1 cut(s) 574
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BciT130I CCWGG 1 cut(s) 592
BclI TGATCA 2 cut(s) 373, 523
BcuI ACTAGT 1 cut(s) 917
BfaI CTAG 1 cut(s) 918
BfmI CTRYAG 2 cut(s) 387, 541
BisI GCNGC 4 cut(s) 305, 384, 706, 738
BlsI GCNGC 4 cut(s) 306, 385, 707, 739
Bme1390I CCNGG 1 cut(s) 592
BmiI GGNNCC 1 cut(s) 861
BmrFI CCNGG 1 cut(s) 592
BmsI GCATC 2 cut(s) 626, 759
BoxI GACNNNNGTC 1 cut(s) 186
BpiI GAAGAC 1 cut(s) 470
Bsa29I ATCGAT 1 cut(s) 329
BsaJI CCNNGG 3 cut(s) 590, 924, 991
BsaWI WCCGGW 1 cut(s) 968
Bsc4I CCNNNNNNNGG 4 cut(s) 101, 591, 948, 968
Bse118I RCCGGY 1 cut(s) 968
BseBI CCWGG 1 cut(s) 592
BseCI ATCGAT 1 cut(s) 329
BseDI CCNNGG 3 cut(s) 590, 924, 991
BseGI GGATG 2 cut(s) 22, 937
BseLI CCNNNNNNNGG 4 cut(s) 101, 591, 948, 968
BseMII CTCAG 1 cut(s) 230
BseRI GAGGAG 5 cut(s) 81, 84, 87, 280, 832
BseSI GKGCMC 1 cut(s) 611
BseXI GCAGC 3 cut(s) 316, 370, 717
BshFI GGCC 4 cut(s) 481, 561, 690, 996
BshNI GGYRCC 1 cut(s) 859
BshTI ACCGGT 1 cut(s) 968
BshVI ATCGAT 1 cut(s) 329
BsiHKAI GWGCWC 1 cut(s) 611
BsiSI CCGG 2 cut(s) 962, 969
BslFI GGGAC 2 cut(s) 174, 998
BslI CCNNNNNNNGG 4 cut(s) 101, 591, 948, 968
BsmFI GGGAC 2 cut(s) 174, 998
BsmI GAATGC 1 cut(s) 500
BsnI GGCC 4 cut(s) 481, 561, 690, 996
Bsp1286I GDGCHC 1 cut(s) 611
Bsp143I GATC 5 cut(s) 268, 373, 523, 647, 658
Bsp19I CCATGG 1 cut(s) 991
BspACI CCGC 2 cut(s) 737, 949
BspANI GGCC 4 cut(s) 481, 561, 690, 996
BspCNI CTCAG 1 cut(s) 229
BspDI ATCGAT 1 cut(s) 329
BspHI TCATGA 3 cut(s) 424, 504, 802
BspLI GGNNCC 1 cut(s) 861
BspPI GGATC 1 cut(s) 653
BspQI GCTCTTC 1 cut(s) 901
BspT107I GGYRCC 1 cut(s) 859
BsrFI RCCGGY 1 cut(s) 968
BssAI RCCGGY 1 cut(s) 968
BssECI CCNNGG 3 cut(s) 590, 924, 991
BssMI GATC 5 cut(s) 268, 373, 523, 647, 658
BssT1I CCWWGG 1 cut(s) 991
Bst2UI CCWGG 1 cut(s) 592
Bst4CI ACNGT 1 cut(s) 185
Bst6I CTCTTC 4 cut(s) 20, 328, 850, 901
BstC8I GCNNGC 1 cut(s) 103
BstDEI CTNAG 1 cut(s) 216
BstDSI CCRYGG 2 cut(s) 924, 991
BstENI CCTNNNNNAGG 1 cut(s) 589
BstF5I GGATG 2 cut(s) 22, 937
BstKTI GATC 5 cut(s) 271, 376, 526, 650, 661
BstMBI GATC 5 cut(s) 268, 373, 523, 647, 658
BstMWI GCNNNNNNNGC 4 cut(s) 643, 743, 752, 983
BstNI CCWGG 1 cut(s) 592
BstNSI RCATGY 1 cut(s) 109
BstPAI GACNNNNGTC 1 cut(s) 186
BstSCI CCNGG 1 cut(s) 590
BstSFI CTRYAG 2 cut(s) 387, 541
BstSLI GKGCMC 1 cut(s) 611
BstV1I GCAGC 3 cut(s) 316, 370, 717
BstV2I GAAGAC 1 cut(s) 470
Bsu15I ATCGAT 1 cut(s) 329
BsuRI GGCC 4 cut(s) 481, 561, 690, 996
BsuTUI ATCGAT 1 cut(s) 329
BtgI CCRYGG 2 cut(s) 924, 991
BtgZI GCGATG 1 cut(s) 686
BtsCI GGATG 2 cut(s) 22, 937
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 2 cut(s) 360, 369
Cac8I GCNNGC 1 cut(s) 103
CciI TCATGA 3 cut(s) 424, 504, 802
Cfr10I RCCGGY 1 cut(s) 968
ClaI ATCGAT 1 cut(s) 329
Csp6I GTAC 3 cut(s) 556, 818, 921
CspAI ACCGGT 1 cut(s) 968
CviAII CATG 6 cut(s) 106, 425, 505, 621, 803, 992
CviQI GTAC 3 cut(s) 556, 818, 921
DdeI CTNAG 1 cut(s) 216
DpnI GATC 5 cut(s) 270, 375, 525, 649, 660
DpnII GATC 5 cut(s) 268, 373, 523, 647, 658
EaeI YGGCCR 1 cut(s) 559
Eam1104I CTCTTC 4 cut(s) 20, 328, 850, 901
EarI CTCTTC 4 cut(s) 20, 328, 850, 901
Eco130I CCWWGG 1 cut(s) 991
Eco32I GATATC 1 cut(s) 333
EcoNI CCTNNNNNAGG 1 cut(s) 589
EcoRII CCWGG 1 cut(s) 590
EcoRV GATATC 1 cut(s) 333
EcoT14I CCWWGG 1 cut(s) 991
ErhI CCWWGG 1 cut(s) 991
FaeI CATG 6 cut(s) 109, 428, 508, 624, 806, 995
FaqI GGGAC 2 cut(s) 174, 998
FatI CATG 6 cut(s) 105, 424, 504, 620, 802, 991
FauNDI CATATG 1 cut(s) 1023
FbaI TGATCA 2 cut(s) 373, 523
Fnu4HI GCNGC 4 cut(s) 305, 384, 706, 738
FokI GGATG 2 cut(s) 29, 924
Fsp4HI GCNGC 4 cut(s) 305, 384, 706, 738
FspBI CTAG 1 cut(s) 918
GluI GCNGC 4 cut(s) 305, 384, 706, 738
HaeIII GGCC 4 cut(s) 481, 561, 690, 996
HapII CCGG 2 cut(s) 962, 969
Hin1II CATG 6 cut(s) 109, 428, 508, 624, 806, 995
HinfI GANTC 3 cut(s) 298, 341, 469
HpaII CCGG 2 cut(s) 962, 969
HphI GGTGA 3 cut(s) 34, 84, 887
Hpy166II GTNNAC 4 cut(s) 80, 358, 609, 906
Hpy188I TCNGA 4 cut(s) 219, 340, 474, 663
Hpy188III TCNNGA 6 cut(s) 425, 505, 530, 803, 941, 1004
Hpy8I GTNNAC 4 cut(s) 80, 358, 609, 906
Hpy99I CGWCG 1 cut(s) 340
HpyAV CCTTC 6 cut(s) 7, 116, 249, 680, 861, 1007
HpyCH4III ACNGT 1 cut(s) 185
HpyCH4V TGCA 5 cut(s) 101, 500, 609, 617, 830
HpyF10VI GCNNNNNNNGC 4 cut(s) 643, 743, 752, 983
HpyF3I CTNAG 1 cut(s) 216
Hsp92II CATG 6 cut(s) 109, 428, 508, 624, 806, 995
Ksp22I TGATCA 2 cut(s) 373, 523
Kzo9I GATC 5 cut(s) 268, 373, 523, 647, 658
LguI GCTCTTC 1 cut(s) 901
LmnI GCTCC 1 cut(s) 643
Lsp1109I GCAGC 3 cut(s) 316, 370, 717
LweI GCATC 2 cut(s) 626, 759
MaeI CTAG 1 cut(s) 918
MaeIII GTNAC 2 cut(s) 596, 673
MalI GATC 5 cut(s) 270, 375, 525, 649, 660
MboI GATC 5 cut(s) 268, 373, 523, 647, 658
MhlI GDGCHC 1 cut(s) 611
MluCI AATT 3 cut(s) 39, 864, 868
MlyI GAGTC 1 cut(s) 478
MmeI TCCRAC 2 cut(s) 154, 318
MseI TTAA 3 cut(s) 42, 776, 867
MspI CCGG 2 cut(s) 962, 969
MspR9I CCNGG 1 cut(s) 592
Mva1269I GAATGC 1 cut(s) 500
MvaI CCWGG 1 cut(s) 592
MwoI GCNNNNNNNGC 4 cut(s) 643, 743, 752, 983
NcoI CCATGG 1 cut(s) 991
NdeI CATATG 1 cut(s) 1023
NdeII GATC 5 cut(s) 268, 373, 523, 647, 658
NlaIII CATG 6 cut(s) 109, 428, 508, 624, 806, 995
NlaIV GGNNCC 1 cut(s) 861
NmeAIII GCCGAG 1 cut(s) 507
NspI RCATGY 1 cut(s) 109
PagI TCATGA 3 cut(s) 424, 504, 802
PciSI GCTCTTC 1 cut(s) 901
PctI GAATGC 1 cut(s) 500
PfeI GAWTC 2 cut(s) 298, 341
PflFI GACNNNGTC 1 cut(s) 1010
PinAI ACCGGT 1 cut(s) 968
PkrI GCNGC 4 cut(s) 306, 385, 707, 739
PleI GAGTC 1 cut(s) 477
PpsI GAGTC 1 cut(s) 477
PshAI GACNNNNGTC 1 cut(s) 186
PshBI ATTAAT 3 cut(s) 42, 776, 867
Psp6I CCWGG 1 cut(s) 590
PspGI CCWGG 1 cut(s) 590
PspN4I GGNNCC 1 cut(s) 861
PsrI GAACNNNNNNTAC 2 cut(s) 781, 813
PsyI GACNNNGTC 1 cut(s) 1010
RsaI GTAC 3 cut(s) 557, 819, 922
RsaNI GTAC 3 cut(s) 556, 818, 921
SapI GCTCTTC 1 cut(s) 901
SaqAI TTAA 3 cut(s) 42, 776, 867
SatI GCNGC 4 cut(s) 305, 384, 706, 738
Sau3AI GATC 5 cut(s) 268, 373, 523, 647, 658
SchI GAGTC 1 cut(s) 478
ScrFI CCNGG 1 cut(s) 592
SduI GDGCHC 1 cut(s) 611
SetI ASST 6 cut(s) 79, 250, 385, 571, 757, 988
SfaNI GCATC 2 cut(s) 626, 759
SfcI CTRYAG 2 cut(s) 387, 541
SpeI ACTAGT 1 cut(s) 917
Sse9I AATT 3 cut(s) 39, 864, 868
SsiI CCGC 2 cut(s) 737, 949
SspMI CTAG 1 cut(s) 918
StyD4I CCNGG 1 cut(s) 590
StyI CCWWGG 1 cut(s) 991
TaaI ACNGT 1 cut(s) 185
TaqI TCGA 3 cut(s) 227, 329, 654
TasI AATT 3 cut(s) 39, 864, 868
TatI WGTACW 1 cut(s) 817
TauI GCSGC 1 cut(s) 740
TfiI GAWTC 2 cut(s) 298, 341
Tru1I TTAA 3 cut(s) 42, 776, 867
Tru9I TTAA 3 cut(s) 42, 776, 867
TscAI CASTG 2 cut(s) 360, 376
TseI GCWGC 3 cut(s) 304, 383, 705
TspRI CASTG 2 cut(s) 360, 376
Tth111I GACNNNGTC 1 cut(s) 1010
VneI GTGCAC 1 cut(s) 607
VspI ATTAAT 3 cut(s) 42, 776, 867
XagI CCTNNNNNAGG 1 cut(s) 589
XceI RCATGY 1 cut(s) 109
XspI CTAG 1 cut(s) 918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.