Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0013220

Physical Location & Seq

Genomic Coordinates Forward (+)

16825441 .. 16826743

Gene Structure 1303 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023023

Sequence Viewer

CDS Protein Promoter (2k)

Length: 948 bp

ATGGAGAAGAAGGATCATTTGATAAAGATGGAGGGCAATACTAGATCCGCATCATTTTCAGAAACTACAGCTGATTATAGCTACCCATCTCCAGGCATGTTTGATTTCTCTCAAGGCGATCAGAAGGACTCGTTAGGGTTTATGGAGCTTTTGGGTATCCAGGACTTTTGTCCTGCTAATTCCCTCTTTGATTTGCCACAGATCACTACACCACAAGTACTGCCGACTACTTCCATTGATATGGCGAAAGAGTACTCTTTCAATCCTTCAACACCAAACTCCTCATCGATTTCATCCGCTTCAAGTGAAGCTGTGAACGAGGAACCACCTACTGATAATAAAGCTGCTGCAGACCAACAAGAGGAAGATGATGGTCATGACCAACCAGAGCCCAAGAAAGGGTTGAAAGCGAATCAGAAGAGGAAGAAGACAACTGAGAAGAAACTAAGAGAGCCGAGATTCGCTTTCATGACAAAGAGTGAGGTTGATCATCTGGAAGATGGATACAGATGGAGAAAGTACGGCCAAAAAGCCGTCAAAAACAGCCCTTTCCCCAGGAGCTACTTTCGGTGCACCAGTGCCTCATGTAATGTGAAGAAGCGTGTCGAGCGATGCGTCAGCGATCCAGGCATCGTTGTGACGACCTATGAAGGCCAACATACTCACCTAAGTCCTCTTATGTTGCCTTCTCCTCCTCCTCCTCCAACTACTGCACCGAATAGCATTATCATGCCAAGAACCCTATTCTCCCAGTATCAACAGCAACAAGGACTGTGGCCATTTGCGGATAATCAAGGCTGCAATTTGGGTTTCTCGACACCGAATGGTACAAACCGGAGTTTCCTTAACGAAAGGTGGTTTTGCAGCCCGGCAGGAGGTTCAGCTTTGATGACAGACCATGGCCTTCTTCAAGACATAGTCCCCTCCAATATGTTGAAGCAAGGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0009719 GO:0009725 GO:0009733 GO:0009889 GO:0010033 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042221 GO:0043207 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

316

Amino Acids

35.08

Weight (kDa)

6.11

Isoelectric Point (pI)

57.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	166 - 222	8.7e-27	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013220)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G62340
fragaria_vesca	FvH4_1g26980
malus_domestica	MD09G1285400.v1.1 MD17G1278100.v1.1
prunus_persica	Prupe.3G008600_v2.0.a1 Prupe.3G008600_v2.0.a1 Prupe.3G008600_v2.0.a1
pyrus_communis	pycom17g27580
rosa_chinensis	RchiOBHm_Chr3g0487201
rosa_laevigata	RLG00000023023
rosa_multiflora	Rmu_ssc0000366.1_g000027
rosa_roxburghii	Rroxscaffold_6G00395030
rosa_rugosa	Rorug03G0232300
rosa_samantha	Rh3AG280200 Rh3BG316200 Rh3CG314300 Rh3DG311600
rosa_wichuraiana	Rw3G024820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	3 cut(s)	48, 297, 785
AclWI	GGATC	3 cut(s)	21, 39, 617
AcoI	YGGCCR	2 cut(s)	523, 776
AfaI	GTAC	4 cut(s)	219, 254, 521, 829
AfiI	CCNNNNNNNGG	5 cut(s)	92, 361, 398, 399, 875
AgsI	TTSAA	6 cut(s)	262, 270, 303, 406, 911, 937
AjnI	CCWGG	4 cut(s)	91, 159, 554, 625
AluBI	AGCT	7 cut(s)	71, 81, 148, 311, 344, 561, 884
AluI	AGCT	7 cut(s)	71, 81, 148, 311, 344, 561, 884
Alw21I	GWGCWC	1 cut(s)	575
Alw44I	GTGCAC	1 cut(s)	571
AlwI	GGATC	3 cut(s)	21, 39, 617
AoxI	GGCC	4 cut(s)	523, 652, 776, 901
ApaLI	GTGCAC	1 cut(s)	571
ApeKI	GCWGC	4 cut(s)	344, 347, 798, 864
AsuC2I	CCSGG	1 cut(s)	869
AsuHPI	GGTGA	1 cut(s)	656
BaeGI	GKGCMC	1 cut(s)	575
BalI	TGGCCA	1 cut(s)	778
BanII	GRGCYC	1 cut(s)	393
BbsI	GAAGAC	1 cut(s)	434
Bbv12I	GWGCWC	1 cut(s)	575
BbvI	GCAGC	4 cut(s)	331, 334, 785, 876
BccI	CCATC	5 cut(s)	22, 94, 365, 494, 504
BceAI	ACGGC	2 cut(s)	518, 538
BciT130I	CCWGG	4 cut(s)	93, 161, 556, 627
BciVI	GTATCC	2 cut(s)	167, 497
BclI	TGATCA	1 cut(s)	487
BcnI	CCSGG	1 cut(s)	869
BfaI	CTAG	1 cut(s)	42
BfmI	CTRYAG	2 cut(s)	66, 348
BfuI	GTATCC	2 cut(s)	167, 497
BisI	GCNGC	4 cut(s)	345, 348, 799, 865
BlsI	GCNGC	4 cut(s)	346, 349, 800, 866
BmcAI	AGTACT	2 cut(s)	219, 254
Bme1390I	CCNGG	5 cut(s)	93, 161, 556, 627, 869
BmiI	GGNNCC	1 cut(s)	324
BmrFI	CCNGG	5 cut(s)	93, 161, 556, 627, 869
BmrI	ACTGGG	1 cut(s)	745
BmsI	GCATC	3 cut(s)	59, 602, 639
BmuI	ACTGGG	1 cut(s)	745
BoxI	GACNNNNGTC	1 cut(s)	168
BpiI	GAAGAC	1 cut(s)	434
BpmI	CTGGAG	1 cut(s)	75
BpuEI	CTTGAG	1 cut(s)	96
BpuMI	CCSGG	1 cut(s)	869
Bsa29I	ATCGAT	1 cut(s)	287
BsaBI	GATNNNNATC	1 cut(s)	49
BsaJI	CCNNGG	2 cut(s)	554, 898
BsaWI	WCCGGW	1 cut(s)	834
Bsc4I	CCNNNNNNNGG	5 cut(s)	92, 361, 398, 399, 875
Bse1I	ACTGG	2 cut(s)	576, 751
Bse8I	GATNNNNATC	1 cut(s)	49
BseBI	CCWGG	4 cut(s)	93, 161, 556, 627
BseCI	ATCGAT	1 cut(s)	287
BseDI	CCNNGG	2 cut(s)	554, 898
BseGI	GGATG	1 cut(s)	293
BseJI	GATNNNNATC	1 cut(s)	49
BseLI	CCNNNNNNNGG	5 cut(s)	92, 361, 398, 399, 875
BseMII	CTCAG	1 cut(s)	426
BseNI	ACTGG	2 cut(s)	576, 751
BseRI	GAGGAG	5 cut(s)	271, 681, 684, 687, 690
BseSI	GKGCMC	1 cut(s)	575
BseXI	GCAGC	4 cut(s)	331, 334, 785, 876
BsgI	GTGCAG	1 cut(s)	696
BshFI	GGCC	4 cut(s)	525, 654, 778, 903
BshVI	ATCGAT	1 cut(s)	287
BsiHKAI	GWGCWC	1 cut(s)	575
BsiSI	CCGG	2 cut(s)	835, 869
BslFI	GGGAC	1 cut(s)	905
BslI	CCNNNNNNNGG	5 cut(s)	92, 361, 398, 399, 875
BsmFI	GGGAC	1 cut(s)	905
BsnI	GGCC	4 cut(s)	525, 654, 778, 903
Bsp1286I	GDGCHC	2 cut(s)	393, 575
Bsp143I	GATC	6 cut(s)	13, 44, 118, 201, 487, 622
Bsp19I	CCATGG	1 cut(s)	898
BspACI	CCGC	3 cut(s)	48, 297, 785
BspANI	GGCC	4 cut(s)	525, 654, 778, 903
BspCNI	CTCAG	1 cut(s)	427
BspDI	ATCGAT	1 cut(s)	287
BspHI	TCATGA	2 cut(s)	376, 468
BspLI	GGNNCC	1 cut(s)	324
BspMAI	CTGCAG	1 cut(s)	352
BspPI	GGATC	3 cut(s)	21, 39, 617
BsrI	ACTGG	2 cut(s)	576, 751
BssECI	CCNNGG	2 cut(s)	554, 898
BssMI	GATC	6 cut(s)	13, 44, 118, 201, 487, 622
BssT1I	CCWWGG	1 cut(s)	898
Bst2UI	CCWGG	4 cut(s)	93, 161, 556, 627
Bst4CI	ACNGT	1 cut(s)	774
Bst6I	CTCTTC	1 cut(s)	413
BstDEI	CTNAG	3 cut(s)	435, 446, 668
BstDSI	CCRYGG	1 cut(s)	898
BstF5I	GGATG	1 cut(s)	293
BstKTI	GATC	6 cut(s)	16, 47, 121, 204, 490, 625
BstMBI	GATC	6 cut(s)	13, 44, 118, 201, 487, 622
BstMWI	GCNNNNNNNGC	2 cut(s)	607, 627
BstNI	CCWGG	4 cut(s)	93, 161, 556, 627
BstNSI	RCATGY	1 cut(s)	100
BstPAI	GACNNNNGTC	1 cut(s)	168
BstSCI	CCNGG	5 cut(s)	91, 159, 554, 625, 867
BstSFI	CTRYAG	2 cut(s)	66, 348
BstSLI	GKGCMC	1 cut(s)	575
BstV1I	GCAGC	4 cut(s)	331, 334, 785, 876
BstV2I	GAAGAC	1 cut(s)	434
BstX2I	RGATCY	1 cut(s)	44
BstXI	CCANNNNNNTGG	1 cut(s)	241
BstYI	RGATCY	1 cut(s)	44
Bsu15I	ATCGAT	1 cut(s)	287
BsuI	GTATCC	2 cut(s)	167, 497
BsuRI	GGCC	4 cut(s)	525, 654, 778, 903
BsuTUI	ATCGAT	1 cut(s)	287
BtgI	CCRYGG	1 cut(s)	898
BtgZI	GCGATG	1 cut(s)	625
BtsCI	GGATG	1 cut(s)	293
BtsIMutI	CAGTG	1 cut(s)	583
CciI	TCATGA	2 cut(s)	376, 468
ClaI	ATCGAT	1 cut(s)	287
CseI	GACGC	1 cut(s)	604
Csp6I	GTAC	4 cut(s)	218, 253, 520, 828
CspCI	CAANNNNNGTGG	2 cut(s)	755, 790
CviAII	CATG	6 cut(s)	97, 377, 469, 585, 730, 899
CviQI	GTAC	4 cut(s)	218, 253, 520, 828
DdeI	CTNAG	3 cut(s)	435, 446, 668
DpnI	GATC	6 cut(s)	15, 46, 120, 203, 489, 624
DpnII	GATC	6 cut(s)	13, 44, 118, 201, 487, 622
EaeI	YGGCCR	2 cut(s)	523, 776
Eam1104I	CTCTTC	1 cut(s)	413
EarI	CTCTTC	1 cut(s)	413
Eco130I	CCWWGG	1 cut(s)	898
Eco24I	GRGCYC	1 cut(s)	393
EcoRII	CCWGG	4 cut(s)	91, 159, 554, 625
EcoT14I	CCWWGG	1 cut(s)	898
EcoT38I	GRGCYC	1 cut(s)	393
ErhI	CCWWGG	1 cut(s)	898
FaeI	CATG	6 cut(s)	100, 380, 472, 588, 733, 902
FaqI	GGGAC	1 cut(s)	905
FatI	CATG	6 cut(s)	96, 376, 468, 584, 729, 898
FbaI	TGATCA	1 cut(s)	487
Fnu4HI	GCNGC	4 cut(s)	345, 348, 799, 865
FokI	GGATG	1 cut(s)	280
FriOI	GRGCYC	1 cut(s)	393
Fsp4HI	GCNGC	4 cut(s)	345, 348, 799, 865
FspBI	CTAG	1 cut(s)	42
GluI	GCNGC	4 cut(s)	345, 348, 799, 865
GsuI	CTGGAG	1 cut(s)	75
HaeIII	GGCC	4 cut(s)	525, 654, 778, 903
HapII	CCGG	2 cut(s)	835, 869
HgaI	GACGC	1 cut(s)	604
Hin1II	CATG	6 cut(s)	100, 380, 472, 588, 733, 902
HinfI	GANTC	3 cut(s)	128, 412, 459
HpaII	CCGG	2 cut(s)	835, 869
HphI	GGTGA	1 cut(s)	656
Hpy166II	GTNNAC	2 cut(s)	316, 573
Hpy188I	TCNGA	3 cut(s)	61, 123, 417
Hpy188III	TCNNGA	5 cut(s)	377, 469, 494, 814, 911
Hpy8I	GTNNAC	2 cut(s)	316, 573
HpyAV	CCTTC	6 cut(s)	4, 118, 276, 644, 696, 914
HpyCH4III	ACNGT	1 cut(s)	774
HpyCH4V	TGCA	5 cut(s)	350, 573, 713, 801, 864
HpyF10VI	GCNNNNNNNGC	2 cut(s)	607, 627
HpyF3I	CTNAG	3 cut(s)	435, 446, 668
Hsp92II	CATG	6 cut(s)	100, 380, 472, 588, 733, 902
Ksp22I	TGATCA	1 cut(s)	487
Kzo9I	GATC	6 cut(s)	13, 44, 118, 201, 487, 622
LmnI	GCTCC	2 cut(s)	145, 558
Lsp1109I	GCAGC	4 cut(s)	331, 334, 785, 876
LweI	GCATC	3 cut(s)	59, 602, 639
MaeI	CTAG	1 cut(s)	42
MaeIII	GTNAC	1 cut(s)	637
MalI	GATC	6 cut(s)	15, 46, 120, 203, 489, 624
MboI	GATC	6 cut(s)	13, 44, 118, 201, 487, 622
MboII	GAAGA	9 cut(s)	19, 377, 430, 436, 439, 451, 509, 607, 899
MflI	RGATCY	1 cut(s)	44
MhlI	GDGCHC	2 cut(s)	393, 575
MlsI	TGGCCA	1 cut(s)	778
MluCI	AATT	2 cut(s)	178, 802
MluNI	TGGCCA	1 cut(s)	778
MlyI	GAGTC	1 cut(s)	122
MmeI	TCCRAC	1 cut(s)	728
Mox20I	TGGCCA	1 cut(s)	778
MscI	TGGCCA	1 cut(s)	778
MseI	TTAA	1 cut(s)	846
MslI	CAYNNNNRTG	3 cut(s)	239, 635, 728
Msp20I	TGGCCA	1 cut(s)	778
MspA1I	CMGCKG	1 cut(s)	71
MspI	CCGG	2 cut(s)	835, 869
MspR9I	CCNGG	5 cut(s)	93, 161, 556, 627, 869
MvaI	CCWGG	4 cut(s)	93, 161, 556, 627
MwoI	GCNNNNNNNGC	2 cut(s)	607, 627
NciI	CCSGG	1 cut(s)	869
NcoI	CCATGG	1 cut(s)	898
NdeII	GATC	6 cut(s)	13, 44, 118, 201, 487, 622
NlaIII	CATG	6 cut(s)	100, 380, 472, 588, 733, 902
NlaIV	GGNNCC	1 cut(s)	324
NmeAIII	GCCGAG	1 cut(s)	480
NmuCI	GTSAC	1 cut(s)	637
NspI	RCATGY	1 cut(s)	100
PagI	TCATGA	2 cut(s)	376, 468
PcsI	WCGNNNNNNNCGW	1 cut(s)	612
PfeI	GAWTC	2 cut(s)	412, 459
PflFI	GACNNNGTC	1 cut(s)	917
PfoI	TCCNGGA	1 cut(s)	159
PkrI	GCNGC	4 cut(s)	346, 349, 800, 866
PleI	GAGTC	1 cut(s)	122
PpsI	GAGTC	1 cut(s)	122
PshAI	GACNNNNGTC	1 cut(s)	168
Psp6I	CCWGG	4 cut(s)	91, 159, 554, 625
PspGI	CCWGG	4 cut(s)	91, 159, 554, 625
PspN4I	GGNNCC	1 cut(s)	324
PstI	CTGCAG	1 cut(s)	352
PsuI	RGATCY	1 cut(s)	44
PsyI	GACNNNGTC	1 cut(s)	917
PvuII	CAGCTG	1 cut(s)	71
RsaI	GTAC	4 cut(s)	219, 254, 521, 829
RsaNI	GTAC	4 cut(s)	218, 253, 520, 828
RseI	CAYNNNNRTG	3 cut(s)	239, 635, 728
SaqAI	TTAA	1 cut(s)	846
SatI	GCNGC	4 cut(s)	345, 348, 799, 865
Sau3AI	GATC	6 cut(s)	13, 44, 118, 201, 487, 622
ScaI	AGTACT	2 cut(s)	219, 254
SchI	GAGTC	1 cut(s)	122
ScrFI	CCNGG	5 cut(s)	93, 161, 556, 627, 869
SduI	GDGCHC	2 cut(s)	393, 575
SfaNI	GCATC	3 cut(s)	59, 602, 639
SfcI	CTRYAG	2 cut(s)	66, 348
SmiMI	CAYNNNNRTG	3 cut(s)	239, 635, 728
SmlI	CTYRAG	1 cut(s)	111
SmoI	CTYRAG	1 cut(s)	111
Sse9I	AATT	2 cut(s)	178, 802
SsiI	CCGC	3 cut(s)	48, 297, 785
SspMI	CTAG	1 cut(s)	42
StyD4I	CCNGG	5 cut(s)	91, 159, 554, 625, 867
StyI	CCWWGG	1 cut(s)	898
TaaI	ACNGT	1 cut(s)	774
TaqI	TCGA	3 cut(s)	287, 606, 815
TasI	AATT	2 cut(s)	178, 802
TatI	WGTACW	2 cut(s)	217, 252
TfiI	GAWTC	2 cut(s)	412, 459
Tru1I	TTAA	1 cut(s)	846
Tru9I	TTAA	1 cut(s)	846
TscAI	CASTG	1 cut(s)	583
TseFI	GTSAC	1 cut(s)	637
TseI	GCWGC	4 cut(s)	344, 347, 798, 864
Tsp45I	GTSAC	1 cut(s)	637
TspDTI	ATGAA	3 cut(s)	282, 457, 663
TspRI	CASTG	1 cut(s)	583
Tth111I	GACNNNGTC	1 cut(s)	917
VneI	GTGCAC	1 cut(s)	571
XceI	RCATGY	1 cut(s)	100
XspI	CTAG	1 cut(s)	42
ZrmI	AGTACT	2 cut(s)	219, 254

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.