Rh3BG316200
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0013220
Physical Location & Seq
Reverse (-)
32340945 .. 32342474
1530 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG316200.1

Sequence Viewer

Length: 939 bp
ATGGAGAAGAAGGATCATTTGCTAAAGATGGAGGGCAATACTAGATCCTCATCATTTTCACAAACTACAGCTGATTATAGCTACCCATTTCCAGGCATGTTTGATTTCTCTCAAGGCGATCAGAAGGACTCGTTAGGGTTTGTGGAGCTTTTGGGTATCCAGGACTTTTGTCCTGCTAATTCCCTCTTTGATTTGCCACAGATCACTACACCACAAGTACTGCCGACTACTTCCATTGATATGGCGAAAGAGTACTCTTTCAATCCTTCAACACCAAACTCCTCATCCATTTCATCCGCTTCAAGTGAAGCTGTGAACGAGGAACCACATACTGATAATAAAGCTGCTGCAGACCAACAAGAAGAAGATGATGGTCATGACCAACCAGAGCCCAAGAAAGGGTTGAAAGCGAATCAGAAGAGGAAGAAGACAACTGAGAAGAAACTAAGAGAGCCGAGATTCGCTTTCATGACAAAGAGTGAGGTTGATCATCTGGAAGATGGATACAGATGGAGAAAGTACGGCCAAAAAGCCGTCAAAAACAGCCCTTTCCCCAGGAGCTACTATCGGTGCACCAGTGCCTCATGTAATGTGAAGAAGCGTGTCGAGCGATGCGTCAACGATCCAGGCATCGTTGTGACGACCTATGAAGGCCAACATACTCACCTAAGTCCTCTTATGTTGCCTCCTCCTCCAACTACTGCACCGAATAGCATTAGCATGCCAAGAACCCTATTCTCCCAGTATCAACAGCAACAAGGACTGCGGCCATTTGTGGATAATCAAGGCTGCAATTTGGGTTTCTCGACACCAAATGGTACAAACCGGAGTTTTCTTAACGAAATGTGGTTTTGCAGCCCGGCAGGAGGTTCAGCTTTGATGACAGACCATGGCCTTCTTCAAGACATAGTCCCCTCCAATATGTTGAAGCAAGAGTAG

Protein Analysis

312

Amino Acids

34.94

Weight (kDa)

6.19

Isoelectric Point (pI)

53.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 166 - 222 2.9e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013220)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62340
fragaria_vesca FvH4_1g26980
malus_domestica MD09G1285400.v1.1 MD17G1278100.v1.1
prunus_persica Prupe.3G008600_v2.0.a1 Prupe.3G008600_v2.0.a1 Prupe.3G008600_v2.0.a1
pyrus_communis pycom17g27580
rosa_chinensis RchiOBHm_Chr3g0487201
rosa_laevigata RLG00000023023
rosa_multiflora Rmu_ssc0000366.1_g000027
rosa_roxburghii Rroxscaffold_6G00395030
rosa_rugosa Rorug03G0232300
rosa_samantha Rh3AG280200 Rh3BG316200 Rh3CG314300 Rh3DG311600
rosa_wichuraiana Rw3G024820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 297, 766
AclWI GGATC 3 cut(s) 21, 39, 617
AcoI YGGCCR 2 cut(s) 523, 767
AfaI GTAC 4 cut(s) 219, 254, 521, 820
AfiI CCNNNNNNNGG 4 cut(s) 92, 398, 399, 866
AgsI TTSAA 6 cut(s) 262, 270, 303, 406, 902, 928
AjnI CCWGG 4 cut(s) 91, 159, 554, 625
AluBI AGCT 7 cut(s) 71, 81, 148, 311, 344, 561, 875
AluI AGCT 7 cut(s) 71, 81, 148, 311, 344, 561, 875
Alw21I GWGCWC 1 cut(s) 575
Alw44I GTGCAC 1 cut(s) 571
AlwI GGATC 3 cut(s) 21, 39, 617
AoxI GGCC 4 cut(s) 523, 652, 767, 892
ApaLI GTGCAC 1 cut(s) 571
ApeKI GCWGC 4 cut(s) 344, 347, 789, 855
AsuC2I CCSGG 1 cut(s) 860
AsuHPI GGTGA 1 cut(s) 656
BaeGI GKGCMC 1 cut(s) 575
BanII GRGCYC 1 cut(s) 393
BbsI GAAGAC 1 cut(s) 434
Bbv12I GWGCWC 1 cut(s) 575
BbvI GCAGC 4 cut(s) 331, 334, 776, 867
BccI CCATC 4 cut(s) 22, 365, 494, 504
BceAI ACGGC 2 cut(s) 518, 538
BciT130I CCWGG 4 cut(s) 93, 161, 556, 627
BciVI GTATCC 2 cut(s) 167, 497
BclI TGATCA 1 cut(s) 487
BcnI CCSGG 1 cut(s) 860
BfaI CTAG 1 cut(s) 42
BfmI CTRYAG 2 cut(s) 66, 348
BfuI GTATCC 2 cut(s) 167, 497
BisI GCNGC 5 cut(s) 345, 348, 767, 790, 856
BlsI GCNGC 5 cut(s) 346, 349, 768, 791, 857
BmcAI AGTACT 2 cut(s) 219, 254
Bme1390I CCNGG 5 cut(s) 93, 161, 556, 627, 860
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 5 cut(s) 93, 161, 556, 627, 860
BmrI ACTGGG 1 cut(s) 736
BmsI GCATC 2 cut(s) 602, 639
BmuI ACTGGG 1 cut(s) 736
BoxI GACNNNNGTC 1 cut(s) 168
BpiI GAAGAC 1 cut(s) 434
BpuEI CTTGAG 1 cut(s) 96
BpuMI CCSGG 1 cut(s) 860
BsaBI GATNNNNATC 1 cut(s) 49
BsaJI CCNNGG 2 cut(s) 554, 889
BsaWI WCCGGW 1 cut(s) 825
Bsc4I CCNNNNNNNGG 4 cut(s) 92, 398, 399, 866
Bse1I ACTGG 2 cut(s) 576, 742
Bse8I GATNNNNATC 1 cut(s) 49
BseBI CCWGG 4 cut(s) 93, 161, 556, 627
BseDI CCNNGG 2 cut(s) 554, 889
BseGI GGATG 2 cut(s) 284, 293
BseJI GATNNNNATC 1 cut(s) 49
BseLI CCNNNNNNNGG 4 cut(s) 92, 398, 399, 866
BseMII CTCAG 1 cut(s) 426
BseNI ACTGG 2 cut(s) 576, 742
BseRI GAGGAG 3 cut(s) 271, 678, 681
BseSI GKGCMC 1 cut(s) 575
BseXI GCAGC 4 cut(s) 331, 334, 776, 867
BsgI GTGCAG 1 cut(s) 687
BshFI GGCC 4 cut(s) 525, 654, 769, 894
BsiHKAI GWGCWC 1 cut(s) 575
BsiSI CCGG 2 cut(s) 826, 860
BslFI GGGAC 1 cut(s) 896
BslI CCNNNNNNNGG 4 cut(s) 92, 398, 399, 866
BsmFI GGGAC 1 cut(s) 896
BsnI GGCC 4 cut(s) 525, 654, 769, 894
Bsp1286I GDGCHC 2 cut(s) 393, 575
Bsp143I GATC 6 cut(s) 13, 44, 118, 201, 487, 622
Bsp19I CCATGG 1 cut(s) 889
BspACI CCGC 2 cut(s) 297, 766
BspANI GGCC 4 cut(s) 525, 654, 769, 894
BspCNI CTCAG 1 cut(s) 427
BspHI TCATGA 2 cut(s) 376, 468
BspLI GGNNCC 1 cut(s) 324
BspMAI CTGCAG 1 cut(s) 352
BspPI GGATC 3 cut(s) 21, 39, 617
BsrI ACTGG 2 cut(s) 576, 742
BssECI CCNNGG 2 cut(s) 554, 889
BssMI GATC 6 cut(s) 13, 44, 118, 201, 487, 622
BssT1I CCWWGG 1 cut(s) 889
Bst2UI CCWGG 4 cut(s) 93, 161, 556, 627
Bst6I CTCTTC 1 cut(s) 413
BstC8I GCNNGC 1 cut(s) 722
BstDEI CTNAG 3 cut(s) 435, 446, 668
BstDSI CCRYGG 1 cut(s) 889
BstF5I GGATG 2 cut(s) 284, 293
BstKTI GATC 6 cut(s) 16, 47, 121, 204, 490, 625
BstMBI GATC 6 cut(s) 13, 44, 118, 201, 487, 622
BstMWI GCNNNNNNNGC 1 cut(s) 607
BstNI CCWGG 4 cut(s) 93, 161, 556, 627
BstNSI RCATGY 2 cut(s) 100, 724
BstPAI GACNNNNGTC 1 cut(s) 168
BstSCI CCNGG 5 cut(s) 91, 159, 554, 625, 858
BstSFI CTRYAG 2 cut(s) 66, 348
BstSLI GKGCMC 1 cut(s) 575
BstV1I GCAGC 4 cut(s) 331, 334, 776, 867
BstV2I GAAGAC 1 cut(s) 434
BstX2I RGATCY 1 cut(s) 44
BstXI CCANNNNNNTGG 1 cut(s) 241
BstYI RGATCY 1 cut(s) 44
BsuI GTATCC 2 cut(s) 167, 497
BsuRI GGCC 4 cut(s) 525, 654, 769, 894
BtgI CCRYGG 1 cut(s) 889
BtgZI GCGATG 1 cut(s) 625
BtsCI GGATG 2 cut(s) 284, 293
BtsIMutI CAGTG 1 cut(s) 583
Cac8I GCNNGC 1 cut(s) 722
CciI TCATGA 2 cut(s) 376, 468
CseI GACGC 1 cut(s) 604
Csp6I GTAC 4 cut(s) 218, 253, 520, 819
CviAII CATG 6 cut(s) 97, 377, 469, 585, 721, 890
CviQI GTAC 4 cut(s) 218, 253, 520, 819
DdeI CTNAG 3 cut(s) 435, 446, 668
DpnI GATC 6 cut(s) 15, 46, 120, 203, 489, 624
DpnII GATC 6 cut(s) 13, 44, 118, 201, 487, 622
EaeI YGGCCR 2 cut(s) 523, 767
Eam1104I CTCTTC 1 cut(s) 413
EarI CTCTTC 1 cut(s) 413
Eco130I CCWWGG 1 cut(s) 889
Eco24I GRGCYC 1 cut(s) 393
EcoRII CCWGG 4 cut(s) 91, 159, 554, 625
EcoT14I CCWWGG 1 cut(s) 889
EcoT38I GRGCYC 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 889
FaeI CATG 6 cut(s) 100, 380, 472, 588, 724, 893
FaqI GGGAC 1 cut(s) 896
FatI CATG 6 cut(s) 96, 376, 468, 584, 720, 889
FbaI TGATCA 1 cut(s) 487
Fnu4HI GCNGC 5 cut(s) 345, 348, 767, 790, 856
FokI GGATG 2 cut(s) 271, 280
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 5 cut(s) 345, 348, 767, 790, 856
FspBI CTAG 1 cut(s) 42
GluI GCNGC 5 cut(s) 345, 348, 767, 790, 856
HaeIII GGCC 4 cut(s) 525, 654, 769, 894
HapII CCGG 2 cut(s) 826, 860
HgaI GACGC 1 cut(s) 604
Hin1II CATG 6 cut(s) 100, 380, 472, 588, 724, 893
HincII GTYRAC 1 cut(s) 619
HindII GTYRAC 1 cut(s) 619
HinfI GANTC 3 cut(s) 128, 412, 459
HpaII CCGG 2 cut(s) 826, 860
HphI GGTGA 1 cut(s) 656
Hpy166II GTNNAC 3 cut(s) 316, 573, 619
Hpy188I TCNGA 2 cut(s) 123, 417
Hpy188III TCNNGA 5 cut(s) 377, 469, 494, 805, 902
Hpy8I GTNNAC 3 cut(s) 316, 573, 619
HpyAV CCTTC 5 cut(s) 4, 118, 276, 644, 905
HpyCH4V TGCA 5 cut(s) 350, 573, 704, 792, 855
HpyF10VI GCNNNNNNNGC 1 cut(s) 607
HpyF3I CTNAG 3 cut(s) 435, 446, 668
Hsp92II CATG 6 cut(s) 100, 380, 472, 588, 724, 893
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 6 cut(s) 13, 44, 118, 201, 487, 622
LmnI GCTCC 2 cut(s) 145, 558
Lsp1109I GCAGC 4 cut(s) 331, 334, 776, 867
LweI GCATC 2 cut(s) 602, 639
MaeI CTAG 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 637
MalI GATC 6 cut(s) 15, 46, 120, 203, 489, 624
MboI GATC 6 cut(s) 13, 44, 118, 201, 487, 622
MflI RGATCY 1 cut(s) 44
MhlI GDGCHC 2 cut(s) 393, 575
MluCI AATT 2 cut(s) 178, 793
MlyI GAGTC 1 cut(s) 122
MmeI TCCRAC 1 cut(s) 719
MseI TTAA 1 cut(s) 837
MslI CAYNNNNRTG 3 cut(s) 239, 635, 719
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 2 cut(s) 826, 860
MspR9I CCNGG 5 cut(s) 93, 161, 556, 627, 860
MvaI CCWGG 4 cut(s) 93, 161, 556, 627
MwoI GCNNNNNNNGC 1 cut(s) 607
NciI CCSGG 1 cut(s) 860
NcoI CCATGG 1 cut(s) 889
NdeII GATC 6 cut(s) 13, 44, 118, 201, 487, 622
NlaIII CATG 6 cut(s) 100, 380, 472, 588, 724, 893
NlaIV GGNNCC 1 cut(s) 324
NmeAIII GCCGAG 1 cut(s) 480
NmuCI GTSAC 1 cut(s) 637
NspI RCATGY 2 cut(s) 100, 724
PaeI GCATGC 1 cut(s) 724
PagI TCATGA 2 cut(s) 376, 468
PcsI WCGNNNNNNNCGW 1 cut(s) 612
PfeI GAWTC 2 cut(s) 412, 459
PflFI GACNNNGTC 1 cut(s) 908
PfoI TCCNGGA 1 cut(s) 159
PkrI GCNGC 5 cut(s) 346, 349, 768, 791, 857
PleI GAGTC 1 cut(s) 122
PpsI GAGTC 1 cut(s) 122
PshAI GACNNNNGTC 1 cut(s) 168
Psp6I CCWGG 4 cut(s) 91, 159, 554, 625
PspGI CCWGG 4 cut(s) 91, 159, 554, 625
PspN4I GGNNCC 1 cut(s) 324
PstI CTGCAG 1 cut(s) 352
PsuI RGATCY 1 cut(s) 44
PsyI GACNNNGTC 1 cut(s) 908
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 4 cut(s) 219, 254, 521, 820
RsaNI GTAC 4 cut(s) 218, 253, 520, 819
RseI CAYNNNNRTG 3 cut(s) 239, 635, 719
SaqAI TTAA 1 cut(s) 837
SatI GCNGC 5 cut(s) 345, 348, 767, 790, 856
Sau3AI GATC 6 cut(s) 13, 44, 118, 201, 487, 622
ScaI AGTACT 2 cut(s) 219, 254
SchI GAGTC 1 cut(s) 122
ScrFI CCNGG 5 cut(s) 93, 161, 556, 627, 860
SduI GDGCHC 2 cut(s) 393, 575
SfaNI GCATC 2 cut(s) 602, 639
SfcI CTRYAG 2 cut(s) 66, 348
SmiMI CAYNNNNRTG 3 cut(s) 239, 635, 719
SmlI CTYRAG 1 cut(s) 111
SmoI CTYRAG 1 cut(s) 111
SphI GCATGC 1 cut(s) 724
Sse9I AATT 2 cut(s) 178, 793
SsiI CCGC 2 cut(s) 297, 766
SspMI CTAG 1 cut(s) 42
StyD4I CCNGG 5 cut(s) 91, 159, 554, 625, 858
StyI CCWWGG 1 cut(s) 889
TaqI TCGA 2 cut(s) 606, 806
TasI AATT 2 cut(s) 178, 793
TatI WGTACW 2 cut(s) 217, 252
TauI GCSGC 1 cut(s) 769
TfiI GAWTC 2 cut(s) 412, 459
Tru1I TTAA 1 cut(s) 837
Tru9I TTAA 1 cut(s) 837
TscAI CASTG 1 cut(s) 583
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 4 cut(s) 344, 347, 789, 855
Tsp45I GTSAC 1 cut(s) 637
TspDTI ATGAA 3 cut(s) 282, 457, 663
TspRI CASTG 1 cut(s) 583
Tth111I GACNNNGTC 1 cut(s) 908
VneI GTGCAC 1 cut(s) 571
XceI RCATGY 2 cut(s) 100, 724
XspI CTAG 1 cut(s) 42
ZrmI AGTACT 2 cut(s) 219, 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.