Rh3CG314300
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0013220
Physical Location & Seq
Reverse (-)
33399363 .. 33400949
1587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG314300.1

Sequence Viewer

Length: 954 bp
ATGGAGAAGAAGGATCATTTGATAAAGATGGAGGGAAATACTAGATCCTCATCATTTTCAGAAACTACAGCTGATTATAGCTACCCATTTCCAGGCATGTTTGATTTCTCTCAAGGCGATCAGAAGGACTCGTTAGGGTTTATGGAGCTTTTGGGTGTCCAGGACTTTTGTCCTGCTAATTCCCTCTTTGATTTGCCACAGATCACTACACCACAAGTACTGCCGACTACTTCCATTGATATGGCGAAAGAGTACTCTTTCAATCCTTCAACACCAAACTCCTCATCGATTTCATCCGCTTCAAGTGAAGCTGTGAACGAGGAACCACATACTGACAAAAAAGCTGCTGCAGACCTACAAGAAGAAGATGATGGTCATGACCAACCAGAGCCCAAGAAAGGGTTGAAAGCGAATCAGAAGAGGAAGAAGACAACTGAGAAGAAACTAAGAGAGCCGAGATTCGCTTTCATGACAAAGAGTGAGGTTGATCATCTGGAAGATGGATACAGATGGAGAAAGTACGGCCAAAAAGCCGTCAAAAACAGCCCTTTCCCCAGGAGCTACTTTCGGTGCACCAGTGCCTCATGTAATGTGAAGAAGCGTGTCGAGCGATGCGTCAACGATCCAGGCATCGTTGTGACGACCTATGAAGGCCAACATACTCACCTAAGTCCTCTTATGTTGCCTCTTCCTCCTCCTCCTCCTCCTCCAACTACTGCACCGAATAGCATTATCATGCCAAGAACCCTATTCTCCCAGTATCAACAGCAACAAGGACTACGGCCGTTTGCGGATAATCAAGGTTGCAATTTGGGTTTCTCGACACCAAATGGTACAAACCGGAGTTTTCTTAACGAAAGGTGGTTTTGCAGCCCGGCAGGAGGTTCAGCTTTGATGACAGACCATGGCCTTCTTCAAGACATAGTCCCCTCCAATATGTTGAAGCAAGGGTAG

Protein Analysis

317

Amino Acids

35.39

Weight (kDa)

6.66

Isoelectric Point (pI)

56.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 166 - 222 5.5e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013220)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62340
fragaria_vesca FvH4_1g26980
malus_domestica MD09G1285400.v1.1 MD17G1278100.v1.1
prunus_persica Prupe.3G008600_v2.0.a1 Prupe.3G008600_v2.0.a1 Prupe.3G008600_v2.0.a1
pyrus_communis pycom17g27580
rosa_chinensis RchiOBHm_Chr3g0487201
rosa_laevigata RLG00000023023
rosa_multiflora Rmu_ssc0000366.1_g000027
rosa_roxburghii Rroxscaffold_6G00395030
rosa_rugosa Rorug03G0232300
rosa_samantha Rh3AG280200 Rh3BG316200 Rh3CG314300 Rh3DG311600
rosa_wichuraiana Rw3G024820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 297, 791
AclWI GGATC 3 cut(s) 21, 39, 617
AcoI YGGCCR 2 cut(s) 523, 782
AfaI GTAC 4 cut(s) 219, 254, 521, 835
AfiI CCNNNNNNNGG 4 cut(s) 92, 398, 399, 881
AgsI TTSAA 6 cut(s) 262, 270, 303, 406, 917, 943
AjnI CCWGG 4 cut(s) 91, 159, 554, 625
AluBI AGCT 7 cut(s) 71, 81, 148, 311, 344, 561, 890
AluI AGCT 7 cut(s) 71, 81, 148, 311, 344, 561, 890
Alw21I GWGCWC 1 cut(s) 575
Alw44I GTGCAC 1 cut(s) 571
AlwI GGATC 3 cut(s) 21, 39, 617
AoxI GGCC 4 cut(s) 523, 652, 782, 907
ApaLI GTGCAC 1 cut(s) 571
ApeKI GCWGC 3 cut(s) 344, 347, 870
AsuC2I CCSGG 1 cut(s) 875
AsuHPI GGTGA 1 cut(s) 656
BaeGI GKGCMC 1 cut(s) 575
BanII GRGCYC 1 cut(s) 393
BbsI GAAGAC 1 cut(s) 434
Bbv12I GWGCWC 1 cut(s) 575
BbvI GCAGC 3 cut(s) 331, 334, 882
BccI CCATC 4 cut(s) 22, 365, 494, 504
BceAI ACGGC 4 cut(s) 518, 538, 769, 797
BciT130I CCWGG 4 cut(s) 93, 161, 556, 627
BciVI GTATCC 1 cut(s) 497
BclI TGATCA 1 cut(s) 487
BcnI CCSGG 1 cut(s) 875
BfaI CTAG 1 cut(s) 42
BfmI CTRYAG 2 cut(s) 66, 348
BfuI GTATCC 1 cut(s) 497
BisI GCNGC 3 cut(s) 345, 348, 871
BlsI GCNGC 3 cut(s) 346, 349, 872
BmcAI AGTACT 2 cut(s) 219, 254
Bme1390I CCNGG 5 cut(s) 93, 161, 556, 627, 875
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 5 cut(s) 93, 161, 556, 627, 875
BmrI ACTGGG 1 cut(s) 751
BmsI GCATC 2 cut(s) 602, 639
BmuI ACTGGG 1 cut(s) 751
BoxI GACNNNNGTC 1 cut(s) 168
BpiI GAAGAC 1 cut(s) 434
BpuEI CTTGAG 1 cut(s) 96
BpuMI CCSGG 1 cut(s) 875
Bsa29I ATCGAT 1 cut(s) 287
BsaBI GATNNNNATC 1 cut(s) 49
BsaJI CCNNGG 2 cut(s) 554, 904
BsaWI WCCGGW 1 cut(s) 840
Bsc4I CCNNNNNNNGG 4 cut(s) 92, 398, 399, 881
Bse1I ACTGG 2 cut(s) 576, 757
Bse8I GATNNNNATC 1 cut(s) 49
BseBI CCWGG 4 cut(s) 93, 161, 556, 627
BseCI ATCGAT 1 cut(s) 287
BseDI CCNNGG 2 cut(s) 554, 904
BseGI GGATG 1 cut(s) 293
BseJI GATNNNNATC 1 cut(s) 49
BseLI CCNNNNNNNGG 4 cut(s) 92, 398, 399, 881
BseMII CTCAG 1 cut(s) 426
BseNI ACTGG 2 cut(s) 576, 757
BseRI GAGGAG 6 cut(s) 271, 684, 687, 690, 693, 696
BseSI GKGCMC 1 cut(s) 575
BseX3I CGGCCG 1 cut(s) 782
BseXI GCAGC 3 cut(s) 331, 334, 882
BsgI GTGCAG 1 cut(s) 702
Bsh1285I CGRYCG 1 cut(s) 785
BshFI GGCC 4 cut(s) 525, 654, 784, 909
BshVI ATCGAT 1 cut(s) 287
BsiEI CGRYCG 1 cut(s) 785
BsiHKAI GWGCWC 1 cut(s) 575
BsiSI CCGG 2 cut(s) 841, 875
BslFI GGGAC 1 cut(s) 911
BslI CCNNNNNNNGG 4 cut(s) 92, 398, 399, 881
BsmFI GGGAC 1 cut(s) 911
BsnI GGCC 4 cut(s) 525, 654, 784, 909
Bsp1286I GDGCHC 2 cut(s) 393, 575
Bsp143I GATC 6 cut(s) 13, 44, 118, 201, 487, 622
Bsp19I CCATGG 1 cut(s) 904
BspACI CCGC 2 cut(s) 297, 791
BspANI GGCC 4 cut(s) 525, 654, 784, 909
BspCNI CTCAG 1 cut(s) 427
BspDI ATCGAT 1 cut(s) 287
BspHI TCATGA 2 cut(s) 376, 468
BspLI GGNNCC 1 cut(s) 324
BspMAI CTGCAG 1 cut(s) 352
BspPI GGATC 3 cut(s) 21, 39, 617
BsrI ACTGG 2 cut(s) 576, 757
BssECI CCNNGG 2 cut(s) 554, 904
BssMI GATC 6 cut(s) 13, 44, 118, 201, 487, 622
BssT1I CCWWGG 1 cut(s) 904
Bst2UI CCWGG 4 cut(s) 93, 161, 556, 627
Bst6I CTCTTC 2 cut(s) 413, 693
BstDEI CTNAG 3 cut(s) 435, 446, 668
BstDSI CCRYGG 1 cut(s) 904
BstF5I GGATG 1 cut(s) 293
BstKTI GATC 6 cut(s) 16, 47, 121, 204, 490, 625
BstMBI GATC 6 cut(s) 13, 44, 118, 201, 487, 622
BstMCI CGRYCG 1 cut(s) 785
BstMWI GCNNNNNNNGC 1 cut(s) 607
BstNI CCWGG 4 cut(s) 93, 161, 556, 627
BstNSI RCATGY 1 cut(s) 100
BstPAI GACNNNNGTC 1 cut(s) 168
BstSCI CCNGG 5 cut(s) 91, 159, 554, 625, 873
BstSFI CTRYAG 2 cut(s) 66, 348
BstSLI GKGCMC 1 cut(s) 575
BstV1I GCAGC 3 cut(s) 331, 334, 882
BstV2I GAAGAC 1 cut(s) 434
BstX2I RGATCY 1 cut(s) 44
BstXI CCANNNNNNTGG 1 cut(s) 241
BstYI RGATCY 1 cut(s) 44
BstZI CGGCCG 1 cut(s) 782
Bsu15I ATCGAT 1 cut(s) 287
BsuI GTATCC 1 cut(s) 497
BsuRI GGCC 4 cut(s) 525, 654, 784, 909
BsuTUI ATCGAT 1 cut(s) 287
BtgI CCRYGG 1 cut(s) 904
BtgZI GCGATG 1 cut(s) 625
BtsCI GGATG 1 cut(s) 293
BtsIMutI CAGTG 1 cut(s) 583
CciI TCATGA 2 cut(s) 376, 468
ClaI ATCGAT 1 cut(s) 287
CseI GACGC 1 cut(s) 604
Csp6I GTAC 4 cut(s) 218, 253, 520, 834
CviAII CATG 6 cut(s) 97, 377, 469, 585, 736, 905
CviQI GTAC 4 cut(s) 218, 253, 520, 834
DdeI CTNAG 3 cut(s) 435, 446, 668
DpnI GATC 6 cut(s) 15, 46, 120, 203, 489, 624
DpnII GATC 6 cut(s) 13, 44, 118, 201, 487, 622
EaeI YGGCCR 2 cut(s) 523, 782
EagI CGGCCG 1 cut(s) 782
Eam1104I CTCTTC 2 cut(s) 413, 693
EarI CTCTTC 2 cut(s) 413, 693
EclXI CGGCCG 1 cut(s) 782
Eco130I CCWWGG 1 cut(s) 904
Eco24I GRGCYC 1 cut(s) 393
Eco52I CGGCCG 1 cut(s) 782
EcoRII CCWGG 4 cut(s) 91, 159, 554, 625
EcoT14I CCWWGG 1 cut(s) 904
EcoT38I GRGCYC 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 904
FaeI CATG 6 cut(s) 100, 380, 472, 588, 739, 908
FaqI GGGAC 1 cut(s) 911
FatI CATG 6 cut(s) 96, 376, 468, 584, 735, 904
FbaI TGATCA 1 cut(s) 487
Fnu4HI GCNGC 3 cut(s) 345, 348, 871
FokI GGATG 1 cut(s) 280
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 3 cut(s) 345, 348, 871
FspBI CTAG 1 cut(s) 42
GluI GCNGC 3 cut(s) 345, 348, 871
HaeIII GGCC 4 cut(s) 525, 654, 784, 909
HapII CCGG 2 cut(s) 841, 875
HgaI GACGC 1 cut(s) 604
Hin1II CATG 6 cut(s) 100, 380, 472, 588, 739, 908
HincII GTYRAC 1 cut(s) 619
HindII GTYRAC 1 cut(s) 619
HinfI GANTC 3 cut(s) 128, 412, 459
HpaII CCGG 2 cut(s) 841, 875
HphI GGTGA 1 cut(s) 656
Hpy166II GTNNAC 3 cut(s) 316, 573, 619
Hpy188I TCNGA 3 cut(s) 61, 123, 417
Hpy188III TCNNGA 5 cut(s) 377, 469, 494, 820, 917
Hpy8I GTNNAC 3 cut(s) 316, 573, 619
HpyAV CCTTC 5 cut(s) 4, 118, 276, 644, 920
HpyCH4V TGCA 5 cut(s) 350, 573, 719, 807, 870
HpyF10VI GCNNNNNNNGC 1 cut(s) 607
HpyF3I CTNAG 3 cut(s) 435, 446, 668
Hsp92II CATG 6 cut(s) 100, 380, 472, 588, 739, 908
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 6 cut(s) 13, 44, 118, 201, 487, 622
LmnI GCTCC 2 cut(s) 145, 558
Lsp1109I GCAGC 3 cut(s) 331, 334, 882
LweI GCATC 2 cut(s) 602, 639
MaeI CTAG 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 637
MalI GATC 6 cut(s) 15, 46, 120, 203, 489, 624
MboI GATC 6 cut(s) 13, 44, 118, 201, 487, 622
MflI RGATCY 1 cut(s) 44
MhlI GDGCHC 2 cut(s) 393, 575
MluCI AATT 2 cut(s) 178, 808
MlyI GAGTC 1 cut(s) 122
MmeI TCCRAC 1 cut(s) 734
MseI TTAA 1 cut(s) 852
MslI CAYNNNNRTG 3 cut(s) 239, 635, 734
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 2 cut(s) 841, 875
MspR9I CCNGG 5 cut(s) 93, 161, 556, 627, 875
MvaI CCWGG 4 cut(s) 93, 161, 556, 627
MwoI GCNNNNNNNGC 1 cut(s) 607
NciI CCSGG 1 cut(s) 875
NcoI CCATGG 1 cut(s) 904
NdeII GATC 6 cut(s) 13, 44, 118, 201, 487, 622
NlaIII CATG 6 cut(s) 100, 380, 472, 588, 739, 908
NlaIV GGNNCC 1 cut(s) 324
NmeAIII GCCGAG 1 cut(s) 480
NmuCI GTSAC 1 cut(s) 637
NspI RCATGY 1 cut(s) 100
PagI TCATGA 2 cut(s) 376, 468
PcsI WCGNNNNNNNCGW 1 cut(s) 612
PfeI GAWTC 2 cut(s) 412, 459
PflFI GACNNNGTC 1 cut(s) 923
PfoI TCCNGGA 1 cut(s) 159
PkrI GCNGC 3 cut(s) 346, 349, 872
PleI GAGTC 1 cut(s) 122
PpsI GAGTC 1 cut(s) 122
PshAI GACNNNNGTC 1 cut(s) 168
Psp6I CCWGG 4 cut(s) 91, 159, 554, 625
PspGI CCWGG 4 cut(s) 91, 159, 554, 625
PspN4I GGNNCC 1 cut(s) 324
PstI CTGCAG 1 cut(s) 352
PsuI RGATCY 1 cut(s) 44
PsyI GACNNNGTC 1 cut(s) 923
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 4 cut(s) 219, 254, 521, 835
RsaNI GTAC 4 cut(s) 218, 253, 520, 834
RseI CAYNNNNRTG 3 cut(s) 239, 635, 734
SaqAI TTAA 1 cut(s) 852
SatI GCNGC 3 cut(s) 345, 348, 871
Sau3AI GATC 6 cut(s) 13, 44, 118, 201, 487, 622
ScaI AGTACT 2 cut(s) 219, 254
SchI GAGTC 1 cut(s) 122
ScrFI CCNGG 5 cut(s) 93, 161, 556, 627, 875
SduI GDGCHC 2 cut(s) 393, 575
SfaNI GCATC 2 cut(s) 602, 639
SfcI CTRYAG 2 cut(s) 66, 348
SmiMI CAYNNNNRTG 3 cut(s) 239, 635, 734
SmlI CTYRAG 1 cut(s) 111
SmoI CTYRAG 1 cut(s) 111
Sse9I AATT 2 cut(s) 178, 808
SsiI CCGC 2 cut(s) 297, 791
SspMI CTAG 1 cut(s) 42
StyD4I CCNGG 5 cut(s) 91, 159, 554, 625, 873
StyI CCWWGG 1 cut(s) 904
TaqI TCGA 3 cut(s) 287, 606, 821
TasI AATT 2 cut(s) 178, 808
TatI WGTACW 2 cut(s) 217, 252
TfiI GAWTC 2 cut(s) 412, 459
Tru1I TTAA 1 cut(s) 852
Tru9I TTAA 1 cut(s) 852
TscAI CASTG 1 cut(s) 583
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 3 cut(s) 344, 347, 870
Tsp45I GTSAC 1 cut(s) 637
TspDTI ATGAA 3 cut(s) 282, 457, 663
TspRI CASTG 1 cut(s) 583
Tth111I GACNNNGTC 1 cut(s) 923
VneI GTGCAC 1 cut(s) 571
XceI RCATGY 1 cut(s) 100
XspI CTAG 1 cut(s) 42
ZrmI AGTACT 2 cut(s) 219, 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.