MD10G1061800.v1.1

RNA polymerase-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003596
Physical Location & Seq
Forward (+)
8328785 .. 8332798
4014 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1061800.v1.1.491

Sequence Viewer

Length: 1974 bp
ATGGCTGAATTAGAAAATTTGCTCTTGGAGGCTGCGGGAAGAACAAGTTCATCGGGGAAAAACCGACACTCACATTCATCATCTAGGAGGAGACGTGGGGGTTCATATTCTGATGGAGGAAGTGACTCCAGAGGTGATGATTCTGATGATGGTCGTGGCTATGCAAGCAGGAAGCCCTCTGGACCTCAAGTTCCTTTGAAGAAGAGGCTGGACCCAACAGAAGTAGACGATGAACAAGGTAGCCAGGAAGAAGGTGGTTATAATGATAGTGGTTCGGATCGTGGTGGGGACAGCAATGAATCTGATGTTGGCAGTGATCTTTACAAGGATGAAGACGATAGGCGGAAGCTTGCAGAGATGTCTGAACTTCAAAGAGAGCTAATTCTGTCTGAAAGAGCACAGAAGAAAGATGACAAGAGTTTAAAGGAGAAGTTCCGACCAAAGTGGGACAAAGGGAAGGCCCCACAGTCCCGGAAAGAGACTCCACCTCTTCCGTCCTCTCGTGTGCGTTCATCAGCCAGATCTGCTGACAGGGCAGCTGCCAAGGATGATGCATTGAATGAGTTACGAGCAAAACGTTTGAAGCAGCAGGACCCTGAGGCTCACCGCAAGTTGAGAGATGCTTCTAGAGGAGGAAGTTCAAGCAGTCGAAGTTTCTCACACCTGAACAGGAAATCCTTCACTGCAGTGGGTCGGAGTAGCTCTAGTCAGAGTGACAGTGATAGTAGGTCTCGTAGTGAAGATGATGGATCATCAGGGGATGATGCAATGATTGACAGTGATGATGAAAGGTCAGAGGGACTAACTTTTGATGATATAAAGGAGATTACCATTCGAAGGTCAAAACTTGCAAAGTGGTTTATGGAGCCGTTCTTTGAAGAGTTAATTGTGGGTTGCTTTGTGAGGGTTGGAATTGGGAGGTCAAAATCTGGGGCTATCTATAGGCTCTGCATGGTCCGGAATGTTGATGCATCAGATCCTGATCGACAGTACAAACTGGAGAACAAAACCACACATAAGTATCTGAATGTTGTTTGGGGCAATGAGAACTCTGCTGCCAGGTGGCAGATGGCTATGATTTCCGACTCAGCGCCGCTGGAGGAGGAGTTTAAACAGTGGATTAGAGAGGTGGAGAGAAGTGGTGGCCGATTGCCAAGCAAAGAGGACGTGTTGGAAAAGAAGGAGGCCATAAAAAAAACCAACTCATTTGTCTACTCAGCCGCAACTGTGAAGCAGATGATACAGGAGAAAAAGTCTGCTTCGTCAAGGCCATTAAACATTGCGCTTGAGAAGGACCGGTTGAGGAGGGAGTTAGAAGTTGCAAAAAGCAAGCAAGATGATGCAGAGGTGGAGAGGATCGAGACAAGATTACAGCAATTAGAAGCACGCCGGCAATCACAGGGGAAAGATAGCAAGGCTATTAGGCTTGCTGAGATGAACAGAAAAAACAGGGTTGAGAATTTCAAGAATGCATCAGAATTAAAACCTAAGAATATAGGTTTGAAAGCGGGGGAGGCCGGTTATGATCCTTTTTCAAGGAGATGGACTAGGTCAAGGAATTACTATGTGTCAAAGCCCGGTGAACAAAAAGAGGAGGCAGAGGCAATAGCAGTGGCTGATGGAGCCTTGGCAGATATAGAAAGTAATGGGACAGCCGTGGTGGGAGCAGTACGGGCTGGCATGGCAGCAACTGCAGCAGCTTTGGAAGCTGCAGCTGGTGCGGGGAAGCTGATTGATACGAGTGCCCCTGTTGATTTTGGAACAGAGTCAAATCTAAAGCATAATTTTGAGCTGCCAATCTCATTGGCTGCTCTTCAAAAGTTTGGCGGGCCACAGGGAGCTCAGAAAGGGTTCCTGGCAAGGAAGCAGCGAATAGAAGCAACAGTTGGGTGCCAAGTCCCGGAGAATGACGGGAGGAGGCATGCACTGACACTTACAGTTAGCGACTACAAGAGAAGAAGAGGGCTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000428 GO:0000988 GO:0000989 GO:0000993 GO:0001076 GO:0001098 GO:0001099 GO:0001701 GO:0001704 GO:0001706 GO:0001711 GO:0001824 GO:0001832 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0007275 GO:0007369 GO:0007492 GO:0008023 GO:0008150 GO:0008152 GO:0008213 GO:0009653 GO:0009790 GO:0009792 GO:0009888 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009909 GO:0009910 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010638 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016591 GO:0016593 GO:0018022 GO:0018023 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0019827 GO:0019899 GO:0030154 GO:0030880 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032259 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0034243 GO:0034968 GO:0035987 GO:0036211 GO:0040007 GO:0043009 GO:0043170 GO:0043175 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045165 GO:0045309 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048581 GO:0048589 GO:0048598 GO:0048646 GO:0048831 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050815 GO:0051093 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051219 GO:0051239 GO:0051241 GO:0051246 GO:0051247 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051568 GO:0051569 GO:0051571 GO:0055029 GO:0060255 GO:0060795 GO:0061695 GO:0065007 GO:0070013 GO:0070063 GO:0071704 GO:0071840 GO:0080090 GO:0080182 GO:0097159 GO:0098727 GO:0099122 GO:0140110 GO:1901363 GO:1901564 GO:1902275 GO:1902494 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905269 GO:1990234 GO:1990269 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Plus-3

Protein Analysis

658

Amino Acids

72.89

Weight (kDa)

9.2

Isoelectric Point (pI)

62.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plus-3 PF03126 272 - 377 2.2e-31 Plus-3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 261
AccB1I GGYRCC 1 cut(s) 1890
AccI GTMKAC 2 cut(s) 225, 1212
AccIII TCCGGA 1 cut(s) 957
AciI CCGC 8 cut(s) 35, 343, 607, 1094, 1221, 1508, 1721, 1827
AclI AACGTT 1 cut(s) 577
AclWI GGATC 5 cut(s) 285, 757, 971, 1364, 1520
AcoI YGGCCR 1 cut(s) 1144
AcsI RAATTY 2 cut(s) 16, 1459
AfaI GTAC 2 cut(s) 992, 1671
AfiI CCNNNNNNNGG 2 cut(s) 837, 1900
AflIII ACRYGT 1 cut(s) 1167
AgeI ACCGGT 1 cut(s) 1296
AjiI CACGTC 2 cut(s) 95, 1168
AjnI CCWGG 3 cut(s) 243, 1058, 1854
AjuI GAANNNNNNNTTGG 4 cut(s) 291, 323, 1869, 1901
AleI CACNNNNGTG 1 cut(s) 686
AloI GAACNNNNNNTCC 4 cut(s) 174, 206, 659, 691
Alw21I GWGCWC 2 cut(s) 400, 1843
Alw26I GTCTC 4 cut(s) 85, 473, 735, 1355
AlwI GGATC 5 cut(s) 285, 757, 971, 1364, 1520
AlwNI CAGNNNCTG 3 cut(s) 980, 1616, 1691
Aor13HI TCCGGA 1 cut(s) 957
AoxI GGCC 6 cut(s) 459, 1144, 1185, 1268, 1515, 1829
ApoI RAATTY 2 cut(s) 16, 1459
AsiGI ACCGGT 1 cut(s) 1296
Asp700I GAANNNNTTC 3 cut(s) 46, 677, 1850
AspLEI GCGC 2 cut(s) 1093, 1285
AspS9I GGNCC 7 cut(s) 182, 211, 460, 592, 955, 1294, 1829
AsuC2I CCSGG 3 cut(s) 472, 1578, 1901
AsuHPI GGTGA 3 cut(s) 146, 596, 1592
AsuII TTCGAA 1 cut(s) 835
AvaII GGWCC 5 cut(s) 182, 211, 592, 955, 1294
AxyI CCTNAGG 1 cut(s) 597
BaeGI GKGCMC 1 cut(s) 1747
BaeI ACNNNNGTAYC 2 cut(s) 1004, 1037
BanI GGYRCC 1 cut(s) 1890
BanII GRGCYC 1 cut(s) 1843
BauI CACGAG 1 cut(s) 501
BbsI GAAGAC 1 cut(s) 339
Bbv12I GWGCWC 2 cut(s) 400, 1843
BccI CCATC 6 cut(s) 107, 143, 740, 1063, 1536, 1613
BceAI ACGGC 2 cut(s) 853, 1640
BciT130I CCWGG 3 cut(s) 245, 1060, 1856
BcnI CCSGG 3 cut(s) 472, 1578, 1901
BcoDI GTCTC 4 cut(s) 85, 473, 735, 1355
BfaI CTAG 4 cut(s) 84, 627, 705, 1548
BfmI CTRYAG 4 cut(s) 684, 940, 1692, 1710
BfoI RGCGCY 1 cut(s) 1094
BglII AGATCT 1 cut(s) 521
Bme1390I CCNGG 6 cut(s) 245, 472, 1060, 1578, 1856, 1901
Bme18I GGWCC 5 cut(s) 182, 211, 592, 955, 1294
BmgBI CACGTC 2 cut(s) 95, 1168
BmgT120I GGNCC 7 cut(s) 182, 211, 460, 592, 955, 1294, 1829
BmiI GGNNCC 7 cut(s) 213, 462, 594, 867, 1624, 1853, 1892
BmrFI CCNGG 6 cut(s) 245, 472, 1060, 1578, 1856, 1901
BmsI GCATC 7 cut(s) 541, 610, 754, 958, 980, 1330, 1481
BpiI GAAGAC 1 cut(s) 339
BplI GAGNNNNNCTC 1 cut(s) 1953
BpmI CTGGAG 3 cut(s) 112, 1019, 1118
Bpu14I TTCGAA 1 cut(s) 835
BpuEI CTTGAG 2 cut(s) 171, 1307
BpuMI CCSGG 3 cut(s) 472, 1578, 1901
BsaBI GATNNNNATC 1 cut(s) 981
BsaI GGTCTC 1 cut(s) 735
BsaJI CCNNGG 3 cut(s) 543, 1626, 1656
BsaWI WCCGGW 2 cut(s) 957, 1296
BsaXI ACNNNNNCTCC 6 cut(s) 79, 109, 1091, 1121, 1238, 1268
Bsc4I CCNNNNNNNGG 2 cut(s) 837, 1900
Bse118I RCCGGY 3 cut(s) 1296, 1389, 1517
Bse1I ACTGG 1 cut(s) 1002
Bse21I CCTNAGG 1 cut(s) 597
Bse3DI GCAATG 4 cut(s) 301, 774, 1048, 1278
Bse8I GATNNNNATC 1 cut(s) 981
BseAI TCCGGA 1 cut(s) 957
BseBI CCWGG 3 cut(s) 245, 1060, 1856
BseDI CCNNGG 3 cut(s) 543, 1626, 1656
BseGI GGATG 3 cut(s) 334, 553, 766
BseJI GATNNNNATC 1 cut(s) 981
BseLI CCNNNNNNNGG 2 cut(s) 837, 1900
BseMI GCAATG 4 cut(s) 301, 774, 1048, 1278
BseMII CTCAG 5 cut(s) 588, 1101, 1230, 1422, 1856
BseNI ACTGG 1 cut(s) 1002
BseRI GAGGAG 7 cut(s) 103, 645, 1115, 1118, 1318, 1607, 1930
BseSI GKGCMC 1 cut(s) 1747
BshFI GGCC 6 cut(s) 461, 1146, 1187, 1270, 1517, 1831
BshNI GGYRCC 1 cut(s) 1890
BshTI ACCGGT 1 cut(s) 1296
BsiHKAI GWGCWC 2 cut(s) 400, 1843
BsiSI CCGG 7 cut(s) 472, 958, 1297, 1390, 1518, 1578, 1901
BslFI GGGAC 6 cut(s) 302, 454, 461, 813, 1663, 1883
BslI CCNNNNNNNGG 2 cut(s) 837, 1900
BsmAI GTCTC 4 cut(s) 85, 473, 735, 1355
BsmBI CGTCTC 1 cut(s) 85
BsmFI GGGAC 6 cut(s) 302, 454, 461, 813, 1663, 1883
BsmI GAATGC 1 cut(s) 1474
BsnI GGCC 6 cut(s) 461, 1146, 1187, 1270, 1517, 1831
Bso31I GGTCTC 1 cut(s) 735
Bsp119I TTCGAA 1 cut(s) 835
Bsp1286I GDGCHC 3 cut(s) 400, 1747, 1843
Bsp13I TCCGGA 1 cut(s) 957
Bsp143I GATC 8 cut(s) 277, 316, 521, 749, 976, 982, 1356, 1525
BspACI CCGC 8 cut(s) 35, 343, 607, 1094, 1221, 1508, 1721, 1827
BspANI GGCC 6 cut(s) 461, 1146, 1187, 1270, 1517, 1831
BspCNI CTCAG 5 cut(s) 589, 1100, 1229, 1423, 1855
BspEI TCCGGA 1 cut(s) 957
BspLI GGNNCC 7 cut(s) 213, 462, 594, 867, 1624, 1853, 1892
BspMAI CTGCAG 3 cut(s) 688, 1696, 1714
BspPI GGATC 5 cut(s) 285, 757, 971, 1364, 1520
BspQI GCTCTTC 1 cut(s) 1818
BspT104I TTCGAA 1 cut(s) 835
BspT107I GGYRCC 1 cut(s) 1890
BspTNI GGTCTC 1 cut(s) 735
BsrDI GCAATG 4 cut(s) 301, 774, 1048, 1278
BsrFI RCCGGY 3 cut(s) 1296, 1389, 1517
BsrI ACTGG 1 cut(s) 1002
BssAI RCCGGY 3 cut(s) 1296, 1389, 1517
BssECI CCNNGG 3 cut(s) 543, 1626, 1656
BssMI GATC 8 cut(s) 277, 316, 521, 749, 976, 982, 1356, 1525
BssSI CACGAG 1 cut(s) 501
BssT1I CCWWGG 2 cut(s) 543, 1626
Bst2BI CACGAG 1 cut(s) 501
Bst2UI CCWGG 3 cut(s) 245, 1060, 1856
Bst4CI ACNGT 8 cut(s) 468, 719, 779, 990, 1116, 1228, 1885, 1939
Bst6I CTCTTC 5 cut(s) 197, 495, 873, 1818, 1954
BstAPI GCANNNNNTGC 2 cut(s) 1691, 1718
BstBI TTCGAA 1 cut(s) 835
BstC8I GCNNGC 9 cut(s) 166, 351, 1331, 1387, 1391, 1428, 1678, 1829, 1923
BstDEI CTNAG 6 cut(s) 597, 1087, 1216, 1431, 1488, 1842
BstDSI CCRYGG 1 cut(s) 1656
BstF5I GGATG 3 cut(s) 334, 553, 766
BstH2I RGCGCY 1 cut(s) 1094
BstHHI GCGC 2 cut(s) 1093, 1285
BstKTI GATC 8 cut(s) 280, 319, 524, 752, 979, 985, 1359, 1528
BstMAI GTCTC 4 cut(s) 85, 473, 735, 1355
BstMBI GATC 8 cut(s) 277, 316, 521, 749, 976, 982, 1356, 1525
BstNI CCWGG 3 cut(s) 245, 1060, 1856
BstNSI RCATGY 1 cut(s) 1925
BstSCI CCNGG 6 cut(s) 243, 470, 1058, 1576, 1854, 1899
BstSFI CTRYAG 4 cut(s) 684, 940, 1692, 1710
BstSLI GKGCMC 1 cut(s) 1747
BstV2I GAAGAC 1 cut(s) 339
BstX2I RGATCY 2 cut(s) 521, 976
BstYI RGATCY 2 cut(s) 521, 976
Bsu36I CCTNAGG 1 cut(s) 597
BsuRI GGCC 6 cut(s) 461, 1146, 1187, 1270, 1517, 1831
BtgI CCRYGG 1 cut(s) 1656
BtrI CACGTC 2 cut(s) 95, 1168
BtsCI GGATG 3 cut(s) 334, 553, 766
BtsI GCAGTG 4 cut(s) 319, 681, 693, 1617
BtsIMutI CAGTG 8 cut(s) 319, 681, 693, 724, 784, 1121, 1617, 1925
Cac8I GCNNGC 9 cut(s) 166, 351, 1331, 1387, 1391, 1428, 1678, 1829, 1923
CaiI CAGNNNCTG 3 cut(s) 980, 1616, 1691
CfoI GCGC 2 cut(s) 1093, 1285
Cfr10I RCCGGY 3 cut(s) 1296, 1389, 1517
Cfr13I GGNCC 7 cut(s) 182, 211, 460, 592, 955, 1294, 1829
Csp6I GTAC 2 cut(s) 991, 1670
CspAI ACCGGT 1 cut(s) 1296
CspCI CAANNNNNGTGG 2 cut(s) 1593, 1628
CviAII CATG 3 cut(s) 952, 1681, 1922
CviQI GTAC 2 cut(s) 991, 1670
DdeI CTNAG 6 cut(s) 597, 1087, 1216, 1431, 1488, 1842
DpnI GATC 8 cut(s) 279, 318, 523, 751, 978, 984, 1358, 1527
DpnII GATC 8 cut(s) 277, 316, 521, 749, 976, 982, 1356, 1525
DraI TTTAAA 2 cut(s) 423, 1111
EaeI YGGCCR 1 cut(s) 1144
Eam1104I CTCTTC 5 cut(s) 197, 495, 873, 1818, 1954
EarI CTCTTC 5 cut(s) 197, 495, 873, 1818, 1954
EciI GGCGGA 1 cut(s) 358
Ecl136II GAGCTC 1 cut(s) 1841
Eco130I CCWWGG 2 cut(s) 543, 1626
Eco24I GRGCYC 1 cut(s) 1843
Eco31I GGTCTC 1 cut(s) 735
Eco47I GGWCC 5 cut(s) 182, 211, 592, 955, 1294
Eco53kI GAGCTC 1 cut(s) 1841
Eco81I CCTNAGG 1 cut(s) 597
EcoICRI GAGCTC 1 cut(s) 1841
EcoO109I RGGNCCY 2 cut(s) 460, 592
EcoRII CCWGG 3 cut(s) 243, 1058, 1854
EcoT14I CCWWGG 2 cut(s) 543, 1626
EcoT22I ATGCAT 3 cut(s) 556, 973, 1474
EcoT38I GRGCYC 1 cut(s) 1843
ErhI CCWWGG 2 cut(s) 543, 1626
Esp3I CGTCTC 1 cut(s) 85
FaeI CATG 3 cut(s) 955, 1684, 1925
FaqI GGGAC 6 cut(s) 302, 454, 461, 813, 1663, 1883
FatI CATG 3 cut(s) 951, 1680, 1921
FauI CCCGC 4 cut(s) 28, 1501, 1714, 1820
FblI GTMKAC 2 cut(s) 225, 1212
FokI GGATG 3 cut(s) 341, 560, 773
FriOI GRGCYC 1 cut(s) 1843
FspBI CTAG 4 cut(s) 84, 627, 705, 1548
GlaI GCGC 2 cut(s) 1092, 1284
GsuI CTGGAG 3 cut(s) 112, 1019, 1118
HaeII RGCGCY 1 cut(s) 1094
HaeIII GGCC 6 cut(s) 461, 1146, 1187, 1270, 1517, 1831
HapII CCGG 7 cut(s) 472, 958, 1297, 1390, 1518, 1578, 1901
HhaI GCGC 2 cut(s) 1093, 1285
Hin1II CATG 3 cut(s) 955, 1684, 1925
Hin6I GCGC 2 cut(s) 1091, 1283
HinP1I GCGC 2 cut(s) 1091, 1283
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 6 cut(s) 125, 140, 299, 481, 1085, 1766
HpaII CCGG 7 cut(s) 472, 958, 1297, 1390, 1518, 1578, 1901
HphI GGTGA 3 cut(s) 146, 596, 1592
Hpy166II GTNNAC 3 cut(s) 226, 1213, 1583
Hpy188III TCNNGA 7 cut(s) 129, 180, 627, 958, 980, 1360, 1465
Hpy8I GTNNAC 3 cut(s) 226, 1213, 1583
HpyAV CCTTC 6 cut(s) 245, 451, 688, 831, 1174, 1285
HpyCH4III ACNGT 8 cut(s) 468, 719, 779, 990, 1116, 1228, 1885, 1939
HpyCH4IV ACGT 3 cut(s) 94, 577, 1167
HpyF3I CTNAG 6 cut(s) 597, 1087, 1216, 1431, 1488, 1842
HpySE526I ACGT 3 cut(s) 94, 577, 1167
Hsp92II CATG 3 cut(s) 955, 1684, 1925
HspAI GCGC 2 cut(s) 1091, 1283
Kpn2I TCCGGA 1 cut(s) 957
KroI GCCGGC 1 cut(s) 1389
KroNI GCCGGC 1 cut(s) 1391
Kzo9I GATC 8 cut(s) 277, 316, 521, 749, 976, 982, 1356, 1525
LguI GCTCTTC 1 cut(s) 1818
LmnI GCTCC 4 cut(s) 865, 1622, 1664, 1838
LweI GCATC 7 cut(s) 541, 610, 754, 958, 980, 1330, 1481
MaeI CTAG 4 cut(s) 84, 627, 705, 1548
MaeII ACGT 3 cut(s) 94, 577, 1167
MaeIII GTNAC 3 cut(s) 122, 564, 713
MalI GATC 8 cut(s) 279, 318, 523, 751, 978, 984, 1358, 1527
MboI GATC 8 cut(s) 277, 316, 521, 749, 976, 982, 1356, 1525
MflI RGATCY 2 cut(s) 521, 976
MhlI GDGCHC 3 cut(s) 400, 1747, 1843
MlyI GAGTC 4 cut(s) 119, 475, 1079, 1775
MmeI TCCRAC 5 cut(s) 460, 674, 889, 1107, 1152
Mph1103I ATGCAT 3 cut(s) 556, 973, 1474
MroI TCCGGA 1 cut(s) 957
MroNI GCCGGC 1 cut(s) 1389
MroXI GAANNNNTTC 3 cut(s) 46, 677, 1850
MseI TTAA 5 cut(s) 422, 884, 1110, 1274, 1481
MslI CAYNNNNRTG 1 cut(s) 686
MspA1I CMGCKG 3 cut(s) 539, 1096, 1715
MspI CCGG 7 cut(s) 472, 958, 1297, 1390, 1518, 1578, 1901
MspR9I CCNGG 6 cut(s) 245, 472, 1060, 1578, 1856, 1901
MssI GTTTAAAC 1 cut(s) 1111
Mva1269I GAATGC 1 cut(s) 1474
MvaI CCWGG 3 cut(s) 245, 1060, 1856
NaeI GCCGGC 1 cut(s) 1391
NciI CCSGG 3 cut(s) 472, 1578, 1901
NdeII GATC 8 cut(s) 277, 316, 521, 749, 976, 982, 1356, 1525
NgoMIV GCCGGC 1 cut(s) 1389
NlaIII CATG 3 cut(s) 955, 1684, 1925
NlaIV GGNNCC 7 cut(s) 213, 462, 594, 867, 1624, 1853, 1892
NmuCI GTSAC 2 cut(s) 122, 713
NsiI ATGCAT 3 cut(s) 556, 973, 1474
NspI RCATGY 1 cut(s) 1925
NspV TTCGAA 1 cut(s) 835
OliI CACNNNNGTG 1 cut(s) 686
PaeI GCATGC 1 cut(s) 1925
PciSI GCTCTTC 1 cut(s) 1818
PcsI WCGNNNNNNNCGW 1 cut(s) 574
PctI GAATGC 1 cut(s) 1474
PdiI GCCGGC 1 cut(s) 1391
PdmI GAANNNNTTC 3 cut(s) 46, 677, 1850
PfeI GAWTC 2 cut(s) 140, 299
PflFI GACNNNGTC 1 cut(s) 1549
PfoI TCCNGGA 2 cut(s) 470, 1899
PinAI ACCGGT 1 cut(s) 1296
PleI GAGTC 4 cut(s) 119, 475, 1079, 1774
PmeI GTTTAAAC 1 cut(s) 1111
PpsI GAGTC 4 cut(s) 119, 475, 1079, 1774
PpuMI RGGWCCY 1 cut(s) 592
PsiI TTATAA 1 cut(s) 261
Psp124BI GAGCTC 1 cut(s) 1843
Psp1406I AACGTT 1 cut(s) 577
Psp5II RGGWCCY 1 cut(s) 592
Psp6I CCWGG 3 cut(s) 243, 1058, 1854
PspGI CCWGG 3 cut(s) 243, 1058, 1854
PspN4I GGNNCC 7 cut(s) 213, 462, 594, 867, 1624, 1853, 1892
PspPI GGNCC 7 cut(s) 182, 211, 460, 592, 955, 1294, 1829
PspPPI RGGWCCY 1 cut(s) 592
PstI CTGCAG 3 cut(s) 688, 1696, 1714
PstNI CAGNNNCTG 3 cut(s) 980, 1616, 1691
PsuI RGATCY 2 cut(s) 521, 976
PsyI GACNNNGTC 1 cut(s) 1549
PvuII CAGCTG 2 cut(s) 539, 1715
RsaI GTAC 2 cut(s) 992, 1671
RsaNI GTAC 2 cut(s) 991, 1670
RseI CAYNNNNRTG 1 cut(s) 686
SacI GAGCTC 1 cut(s) 1843
SapI GCTCTTC 1 cut(s) 1818
SaqAI TTAA 5 cut(s) 422, 884, 1110, 1274, 1481
Sau3AI GATC 8 cut(s) 277, 316, 521, 749, 976, 982, 1356, 1525
Sau96I GGNCC 7 cut(s) 182, 211, 460, 592, 955, 1294, 1829
SchI GAGTC 4 cut(s) 119, 475, 1079, 1775
ScrFI CCNGG 6 cut(s) 245, 472, 1060, 1578, 1856, 1901
SduI GDGCHC 3 cut(s) 400, 1747, 1843
SfaNI GCATC 7 cut(s) 541, 610, 754, 958, 980, 1330, 1481
SfcI CTRYAG 4 cut(s) 684, 940, 1692, 1710
SfuI TTCGAA 1 cut(s) 835
SinI GGWCC 5 cut(s) 182, 211, 592, 955, 1294
SmiMI CAYNNNNRTG 1 cut(s) 686
SmlI CTYRAG 2 cut(s) 186, 1286
SmoI CTYRAG 2 cut(s) 186, 1286
SphI GCATGC 1 cut(s) 1925
SsiI CCGC 8 cut(s) 35, 343, 607, 1094, 1221, 1508, 1721, 1827
SspMI CTAG 4 cut(s) 84, 627, 705, 1548
SstI GAGCTC 1 cut(s) 1843
StyD4I CCNGG 6 cut(s) 243, 470, 1058, 1576, 1854, 1899
StyI CCWWGG 2 cut(s) 543, 1626
TaaI ACNGT 8 cut(s) 468, 719, 779, 990, 1116, 1228, 1885, 1939
TaiI ACGT 3 cut(s) 97, 580, 1170
TaqI TCGA 4 cut(s) 649, 835, 985, 1359
TatI WGTACW 1 cut(s) 990
TauI GCSGC 2 cut(s) 1096, 1223
TfiI GAWTC 2 cut(s) 140, 299
Tru1I TTAA 5 cut(s) 422, 884, 1110, 1274, 1481
Tru9I TTAA 5 cut(s) 422, 884, 1110, 1274, 1481
TscAI CASTG 8 cut(s) 319, 688, 693, 724, 784, 1121, 1617, 1932
TseFI GTSAC 2 cut(s) 122, 713
Tsp45I GTSAC 2 cut(s) 122, 713
TspDTI ATGAA 9 cut(s) 39, 66, 93, 246, 312, 345, 501, 801, 1451
TspGWI ACGGA 1 cut(s) 483
TspRI CASTG 8 cut(s) 319, 688, 693, 724, 784, 1121, 1617, 1932
Tth111I GACNNNGTC 1 cut(s) 1549
VpaK11BI GGWCC 5 cut(s) 182, 211, 592, 955, 1294
XapI RAATTY 2 cut(s) 16, 1459
XbaI TCTAGA 1 cut(s) 626
XceI RCATGY 1 cut(s) 1925
XcmI CCANNNNNNNNNTGG 2 cut(s) 251, 1066
XmiI GTMKAC 2 cut(s) 225, 1212
XmnI GAANNNNTTC 3 cut(s) 46, 677, 1850
XspI CTAG 4 cut(s) 84, 627, 705, 1548
Zsp2I ATGCAT 3 cut(s) 556, 973, 1474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.