pycom12g19070

RNA polymerase-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0003596
Physical Location & Seq
Reverse (-)
21033458 .. 21035390
1933 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g19070.1

Sequence Viewer

Length: 1848 bp
ATGTCAGACTTGGAATTGGAAAGTGACCTCCTTGAGGCCGCAGGAAGAACTAGTTCGGCTGGGAAAAAGCGGGACCGGCTTCCACCTTCTAGAAAGCAACGTGGAGGTTCATATTCTGATGATGGAAGTGACTCCAGAGGTGAAGACTCAGATGATGATCGTGGAAGCAGGAAGCCCTCTCAAGGTGAAAATAGTGATGGATCTGATTTTGGCCCTGATCTTTACAAGAATGATGCTGACAGGAAACGGCTTGCAGCAATGAGTGAACTTGAAAGAGAAATGATTTTGACAAATCGAGCAGAGAAGAAAGGCGGTAAGGATTTCATGGAGAAATTGAGATCAAAGAGAGGCAATGCAAAATCTACCCAATCAAGGAAAGAGGACCCTCTCCCCTTTTCTCGCGGTGCACGCTCATCAGCTCGGGCTGCTGACAAGTCAGCGGCTATGGATGATGCATTGAATAAATTGCGAGCAAAACGTATGAAGCAACAAGACTCAAAGGATCACCATAAGTTAAGGGACACATCAAAAGGAGGTGCAGGTAGCCAGGATATTTCACCAACCAAGCATCGCTCCATTCAAACAGAGACTCTGGACGGGTTGCCTAATGAAGAGGAAGCATTGAATGCGGATGATGAATTCCTTGACAGCGATGAGGAAAGTTCAGAGCTACTGACATTTCAGGACATAAAGGATATTACCATTCCAAGGTCAAAACTTGCAAAATGGGTTATGGAACCATTTTTTGAGGAGTTAATCGTGGGCTGCTTTGTTAGGGTTGGATTCGGCATATCATCAGAAGGTCCTGTCTACAGGCTTTGCATGGTTCAGAACGTGGTTGCCACAAATCCTGGCAGGGAGTACAAGTTAGAGGGTAAAGTCACACATAAATACTTGAATTGTGTTTGGGGCAGTGAAAGTTCTGCTTCCAAATGGCCGATGGCTAATGTCTCAGATTCTCCTCCGCAAGAGAAGGAGTTTGAACAGTGGCTTAAGGAAGTCAAGCGTGCTGGCCAAATGCCAAGCAAACAGGAGGTGGTAGAAAAGAAGGATACCCTTCTGAAATCGAATACATTTGTTTACTCAGCAGCCACTGTGAAGCAAATGTTGCAGGACAAAAAAGTGTCAGCAAGGCCAGTTAACGTTGCAGTTGAGAAGGAACGGTTGAGGAGGCAGTTGGATGCTGCAGAGAGTAAAGGCAACGATGAGGAAGTTGAGAGGATCAAGACAAGGCTCCAGGAATTAGAAGCATCCAGGCAAACTCAGGGGACAGATAGCAAGGCTTTTAGATTAGCTGAAATGAACAGGAAGAACAGGTTCGAGAATTTTATAAATGCTTCGGGTTTGAAACCAAGAAATATGAATCTGAAAGCGGGGGAGGCTGGTTATGATCCATTCTCGAGAAGATGGACTCGCTCGAGAAATTACTATGATGCAAGGCCTGGAGGAGGCGATGAAGCTGCTGAGGCAACGGATGGAACTACCGGAACAGGCAGCAACGGGGAAAAGGCAAATGTAACAGGAGAGCTTGGCATGCAAGCAACAGAGGCAGCATTGGAAGCAGCAGCCGGTGCAGGGAAGTTGGTTGATACAAGTGCTCCTGTGGACAAAGGGACAGAATCATACATGCTGCACAATTTCGAGCTGCCAATCTCGTTAGCTCCACTTCAGAAGTACGGTGGACCTCAGGGTGCTGGGATCGCGTATATGGAGAGAAGACAAAGGATCGAAGCAACAGTCGGGCGTCGAGTGCTGGATGACGGGAGGAGGCATGCCTCGGCCTTGACTATTAAGGACTATATGAGACGACAGGAGATGCACCACGCTCACCCTGACCCTGATTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Plus-3

Protein Analysis

616

Amino Acids

68.35

Weight (kDa)

7.15

Isoelectric Point (pI)

51.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plus-3 PF03126 229 - 334 4.6e-30 Plus-3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1331
Acc36I ACCTGC 1 cut(s) 530
AccI GTMKAC 1 cut(s) 810
AccII CGCG 2 cut(s) 402, 1703
AciI CCGC 8 cut(s) 39, 70, 312, 402, 440, 629, 965, 1373
AclI AACGTT 1 cut(s) 1143
AclWI GGATC 6 cut(s) 208, 510, 1229, 1385, 1706, 1733
AcoI YGGCCR 2 cut(s) 935, 1012
AcsI RAATTY 2 cut(s) 638, 1324
AcuI CTGAAG 1 cut(s) 1652
AcyI GRCGYC 1 cut(s) 1744
AfaI GTAC 2 cut(s) 863, 1676
AfiI CCNNNNNNNGG 6 cut(s) 34, 182, 372, 708, 1448, 1575
AflII CTTAAG 1 cut(s) 992
AgsI TTSAA 7 cut(s) 272, 460, 581, 625, 898, 983, 1348
AhlI ACTAGT 1 cut(s) 50
AjnI CCWGG 5 cut(s) 546, 850, 1236, 1253, 1441
AluBI AGCT 7 cut(s) 419, 670, 1295, 1460, 1528, 1645, 1661
AluI AGCT 7 cut(s) 419, 670, 1295, 1460, 1528, 1645, 1661
Alw21I GWGCWC 2 cut(s) 409, 1600
Alw26I GTCTC 3 cut(s) 581, 955, 1798
Alw44I GTGCAC 1 cut(s) 405
AlwI GGATC 6 cut(s) 208, 510, 1229, 1385, 1706, 1733
AlwNI CAGNNNCTG 1 cut(s) 1094
Ama87I CYCGRG 3 cut(s) 420, 1399, 1417
AoxI GGCC 7 cut(s) 36, 211, 935, 1012, 1133, 1439, 1779
ApaLI GTGCAC 1 cut(s) 405
ApoI RAATTY 2 cut(s) 638, 1324
ArsI GACNNNNNNTTYG 2 cut(s) 1722, 1754
Asp700I GAANNNNTTC 2 cut(s) 52, 1316
AspS9I GGNCC 5 cut(s) 73, 212, 382, 803, 1682
AsuHPI GGTGA 5 cut(s) 152, 197, 497, 549, 1820
AvaI CYCGRG 3 cut(s) 420, 1399, 1417
AvaII GGWCC 4 cut(s) 73, 382, 803, 1682
AxyI CCTNAGG 1 cut(s) 1686
BaeGI GKGCMC 1 cut(s) 409
BalI TGGCCA 1 cut(s) 1014
BbsI GAAGAC 2 cut(s) 150, 1723
Bbv12I GWGCWC 2 cut(s) 409, 1600
BbvCI CCTCAGC 1 cut(s) 1464
BccI CCATC 5 cut(s) 116, 191, 934, 1401, 1469
BceAI ACGGC 1 cut(s) 263
BcgI CGANNNNNNTGC 2 cut(s) 1442, 1476
BciT130I CCWGG 5 cut(s) 548, 852, 1238, 1255, 1443
BciVI GTATCC 1 cut(s) 1045
BcoDI GTCTC 3 cut(s) 581, 955, 1798
BcuI ACTAGT 1 cut(s) 50
BfaI CTAG 2 cut(s) 51, 90
BfmI CTRYAG 2 cut(s) 811, 1185
BfrI CTTAAG 1 cut(s) 992
BfuAI ACCTGC 1 cut(s) 530
BfuI GTATCC 1 cut(s) 1045
Bme1390I CCNGG 5 cut(s) 548, 852, 1238, 1255, 1443
Bme18I GGWCC 4 cut(s) 73, 382, 803, 1682
BmeT110I CYCGRG 3 cut(s) 420, 1399, 1417
BmgT120I GGNCC 5 cut(s) 73, 212, 382, 803, 1682
BmiI GGNNCC 4 cut(s) 74, 384, 738, 1235
BmrFI CCNGG 5 cut(s) 548, 852, 1238, 1255, 1443
BmsI GCATC 7 cut(s) 223, 442, 577, 1171, 1259, 1423, 1806
BpiI GAAGAC 2 cut(s) 150, 1723
BpmI CTGGAG 3 cut(s) 118, 1220, 1464
Bpu10I CCTNAGC 1 cut(s) 1464
BpuEI CTTGAG 2 cut(s) 53, 165
BsaBI GATNNNNATC 1 cut(s) 156
BsaHI GRCGYC 1 cut(s) 1744
BsaJI CCNNGG 2 cut(s) 707, 1776
BsaWI WCCGGW 1 cut(s) 1484
BsaXI ACNNNNNCTCC 4 cut(s) 851, 881, 1582, 1612
Bsc4I CCNNNNNNNGG 6 cut(s) 34, 182, 372, 708, 1448, 1575
Bse118I RCCGGY 2 cut(s) 75, 1568
Bse1I ACTGG 1 cut(s) 1136
Bse21I CCTNAGG 1 cut(s) 1686
Bse3DI GCAATG 2 cut(s) 264, 358
Bse8I GATNNNNATC 1 cut(s) 156
BseBI CCWGG 5 cut(s) 548, 852, 1238, 1255, 1443
BseDI CCNNGG 2 cut(s) 707, 1776
BseGI GGATG 6 cut(s) 454, 637, 1186, 1250, 1480, 1762
BseJI GATNNNNATC 1 cut(s) 156
BseLI CCNNNNNNNGG 6 cut(s) 34, 182, 372, 708, 1448, 1575
BseMI GCAATG 2 cut(s) 264, 358
BseMII CTCAG 6 cut(s) 162, 966, 1098, 1277, 1455, 1700
BseNI ACTGG 1 cut(s) 1136
BseRI GAGGAG 5 cut(s) 764, 951, 1183, 1461, 1780
BseSI GKGCMC 1 cut(s) 409
BseYI CCCAGC 2 cut(s) 59, 1694
BsgI GTGCAG 3 cut(s) 558, 1593, 1616
Bsh1236I CGCG 2 cut(s) 402, 1703
BshFI GGCC 7 cut(s) 38, 213, 937, 1014, 1135, 1441, 1781
BsiHKAI GWGCWC 2 cut(s) 409, 1600
BsiHKCI CYCGRG 3 cut(s) 420, 1399, 1417
BsiSI CCGG 3 cut(s) 76, 1485, 1569
BslFI GGGAC 4 cut(s) 86, 533, 1282, 1627
BslI CCNNNNNNNGG 6 cut(s) 34, 182, 372, 708, 1448, 1575
BsmAI GTCTC 3 cut(s) 581, 955, 1798
BsmBI CGTCTC 1 cut(s) 1798
BsmFI GGGAC 4 cut(s) 86, 533, 1282, 1627
BsmI GAATGC 1 cut(s) 631
BsnI GGCC 7 cut(s) 38, 213, 937, 1014, 1135, 1441, 1781
BsoBI CYCGRG 3 cut(s) 420, 1399, 1417
Bsp1286I GDGCHC 2 cut(s) 409, 1600
Bsp143I GATC 9 cut(s) 157, 200, 217, 338, 502, 1221, 1390, 1698, 1725
BspACI CCGC 8 cut(s) 39, 70, 312, 402, 440, 629, 965, 1373
BspANI GGCC 7 cut(s) 38, 213, 937, 1014, 1135, 1441, 1781
BspCNI CTCAG 6 cut(s) 161, 965, 1097, 1276, 1456, 1699
BspFNI CGCG 2 cut(s) 402, 1703
BspLI GGNNCC 4 cut(s) 74, 384, 738, 1235
BspMAI CTGCAG 1 cut(s) 1189
BspMI ACCTGC 1 cut(s) 530
BspPI GGATC 6 cut(s) 208, 510, 1229, 1385, 1706, 1733
BspTI CTTAAG 1 cut(s) 992
BsrDI GCAATG 2 cut(s) 264, 358
BsrFI RCCGGY 2 cut(s) 75, 1568
BsrI ACTGG 1 cut(s) 1136
BssAI RCCGGY 2 cut(s) 75, 1568
BssECI CCNNGG 2 cut(s) 707, 1776
BssMI GATC 9 cut(s) 157, 200, 217, 338, 502, 1221, 1390, 1698, 1725
BssNI GRCGYC 1 cut(s) 1744
BssT1I CCWWGG 1 cut(s) 707
Bst2UI CCWGG 5 cut(s) 548, 852, 1238, 1255, 1443
Bst4CI ACNGT 5 cut(s) 987, 1096, 1164, 1679, 1738
Bst6I CTCTTC 1 cut(s) 606
BstACI GRCGYC 1 cut(s) 1744
BstAFI CTTAAG 1 cut(s) 992
BstAPI GCANNNNNTGC 3 cut(s) 626, 1108, 1571
BstC8I GCNNGC 8 cut(s) 252, 409, 471, 1008, 1012, 1535, 1539, 1773
BstDEI CTNAG 6 cut(s) 148, 952, 1084, 1263, 1464, 1686
BstENI CCTNNNNNAGG 2 cut(s) 32, 1446
BstF5I GGATG 6 cut(s) 454, 637, 1186, 1250, 1480, 1762
BstFNI CGCG 2 cut(s) 402, 1703
BstKTI GATC 9 cut(s) 160, 203, 220, 341, 505, 1224, 1393, 1701, 1728
BstMAI GTCTC 3 cut(s) 581, 955, 1798
BstMBI GATC 9 cut(s) 157, 200, 217, 338, 502, 1221, 1390, 1698, 1725
BstNI CCWGG 5 cut(s) 548, 852, 1238, 1255, 1443
BstNSI RCATGY 3 cut(s) 1537, 1630, 1775
BstSCI CCNGG 5 cut(s) 546, 850, 1236, 1253, 1441
BstSFI CTRYAG 2 cut(s) 811, 1185
BstSLI GKGCMC 1 cut(s) 409
BstUI CGCG 2 cut(s) 402, 1703
BstV2I GAAGAC 2 cut(s) 150, 1723
BstX2I RGATCY 1 cut(s) 200
BstYI RGATCY 1 cut(s) 200
Bsu36I CCTNAGG 1 cut(s) 1686
BsuI GTATCC 1 cut(s) 1045
BsuRI GGCC 7 cut(s) 38, 213, 937, 1014, 1135, 1441, 1781
BtgZI GCGATG 3 cut(s) 554, 666, 1467
BtsCI GGATG 6 cut(s) 454, 637, 1186, 1250, 1480, 1762
BtsI GCAGTG 1 cut(s) 919
BtsIMutI CAGTG 3 cut(s) 919, 992, 1092
BveI ACCTGC 1 cut(s) 530
Cac8I GCNNGC 8 cut(s) 252, 409, 471, 1008, 1012, 1535, 1539, 1773
CaiI CAGNNNCTG 1 cut(s) 1094
Cfr10I RCCGGY 2 cut(s) 75, 1568
Cfr13I GGNCC 5 cut(s) 73, 212, 382, 803, 1682
CseI GACGC 1 cut(s) 1733
Csp6I GTAC 2 cut(s) 862, 1675
CviAII CATG 5 cut(s) 325, 823, 1534, 1627, 1772
CviQI GTAC 2 cut(s) 862, 1675
DdeI CTNAG 6 cut(s) 148, 952, 1084, 1263, 1464, 1686
DpnI GATC 9 cut(s) 159, 202, 219, 340, 504, 1223, 1392, 1700, 1727
DpnII GATC 9 cut(s) 157, 200, 217, 338, 502, 1221, 1390, 1698, 1725
EaeI YGGCCR 2 cut(s) 935, 1012
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
Eco130I CCWWGG 1 cut(s) 707
Eco147I AGGCCT 1 cut(s) 1441
Eco47I GGWCC 4 cut(s) 73, 382, 803, 1682
Eco57I CTGAAG 1 cut(s) 1652
Eco81I CCTNAGG 1 cut(s) 1686
Eco88I CYCGRG 3 cut(s) 420, 1399, 1417
EcoNI CCTNNNNNAGG 2 cut(s) 32, 1446
EcoO109I RGGNCCY 2 cut(s) 382, 803
EcoRI GAATTC 1 cut(s) 638
EcoRII CCWGG 5 cut(s) 546, 850, 1236, 1253, 1441
EcoT14I CCWWGG 1 cut(s) 707
EcoT22I ATGCAT 1 cut(s) 457
ErhI CCWWGG 1 cut(s) 707
Esp3I CGTCTC 1 cut(s) 1798
FaeI CATG 5 cut(s) 328, 826, 1537, 1630, 1775
FalI AAGNNNNNCTT 2 cut(s) 910, 942
FaqI GGGAC 4 cut(s) 86, 533, 1282, 1627
FatI CATG 5 cut(s) 324, 822, 1533, 1626, 1771
FauI CCCGC 2 cut(s) 63, 1366
FblI GTMKAC 1 cut(s) 810
FokI GGATG 6 cut(s) 461, 644, 1193, 1237, 1487, 1769
FspBI CTAG 2 cut(s) 51, 90
GsaI CCCAGC 2 cut(s) 63, 1698
GsuI CTGGAG 3 cut(s) 118, 1220, 1464
HaeIII GGCC 7 cut(s) 38, 213, 937, 1014, 1135, 1441, 1781
HapII CCGG 3 cut(s) 76, 1485, 1569
HgaI GACGC 1 cut(s) 1733
Hin1I GRCGYC 1 cut(s) 1744
Hin1II CATG 5 cut(s) 328, 826, 1537, 1630, 1775
HincII GTYRAC 1 cut(s) 1141
HindII GTYRAC 1 cut(s) 1141
HpaI GTTAAC 1 cut(s) 1141
HpaII CCGG 3 cut(s) 76, 1485, 1569
HphI GGTGA 5 cut(s) 152, 197, 497, 549, 1820
Hpy166II GTNNAC 7 cut(s) 266, 407, 811, 1081, 1141, 1606, 1682
Hpy188III TCNNGA 9 cut(s) 90, 135, 593, 683, 1225, 1321, 1399, 1401, 1419
Hpy8I GTNNAC 7 cut(s) 266, 407, 811, 1081, 1141, 1606, 1682
Hpy99I CGWCG 1 cut(s) 1749
HpyAV CCTTC 6 cut(s) 96, 794, 967, 1042, 1067, 1150
HpyCH4III ACNGT 5 cut(s) 987, 1096, 1164, 1679, 1738
HpyCH4IV ACGT 4 cut(s) 100, 478, 834, 1143
HpyF3I CTNAG 6 cut(s) 148, 952, 1084, 1263, 1464, 1686
HpySE526I ACGT 4 cut(s) 100, 478, 834, 1143
Hsp92I GRCGYC 1 cut(s) 1744
Hsp92II CATG 5 cut(s) 328, 826, 1537, 1630, 1775
KspAI GTTAAC 1 cut(s) 1141
Kzo9I GATC 9 cut(s) 157, 200, 217, 338, 502, 1221, 1390, 1698, 1725
LmnI GCTCC 4 cut(s) 578, 1239, 1603, 1666
LweI GCATC 7 cut(s) 223, 442, 577, 1171, 1259, 1423, 1806
MaeI CTAG 2 cut(s) 51, 90
MaeII ACGT 4 cut(s) 100, 478, 834, 1143
MaeIII GTNAC 4 cut(s) 23, 128, 880, 1516
MalI GATC 9 cut(s) 159, 202, 219, 340, 504, 1223, 1392, 1700, 1727
MboI GATC 9 cut(s) 157, 200, 217, 338, 502, 1221, 1390, 1698, 1725
MboII GAAGA 7 cut(s) 57, 155, 316, 623, 1321, 1416, 1728
MflI RGATCY 1 cut(s) 200
MhlI GDGCHC 2 cut(s) 409, 1600
MlsI TGGCCA 1 cut(s) 1014
MluCI AATT 9 cut(s) 14, 332, 464, 638, 898, 1241, 1324, 1423, 1636
MluNI TGGCCA 1 cut(s) 1014
MlyI GAGTC 5 cut(s) 125, 140, 488, 583, 1405
MmeI TCCRAC 2 cut(s) 760, 1158
Mox20I TGGCCA 1 cut(s) 1014
Mph1103I ATGCAT 1 cut(s) 457
MroXI GAANNNNTTC 2 cut(s) 52, 1316
MscI TGGCCA 1 cut(s) 1014
MseI TTAA 5 cut(s) 515, 755, 993, 1140, 1791
Msp20I TGGCCA 1 cut(s) 1014
MspA1I CMGCKG 1 cut(s) 440
MspCI CTTAAG 1 cut(s) 992
MspI CCGG 3 cut(s) 76, 1485, 1569
MspR9I CCNGG 5 cut(s) 548, 852, 1238, 1255, 1443
Mva1269I GAATGC 1 cut(s) 631
MvaI CCWGG 5 cut(s) 548, 852, 1238, 1255, 1443
MvnI CGCG 2 cut(s) 402, 1703
NdeII GATC 9 cut(s) 157, 200, 217, 338, 502, 1221, 1390, 1698, 1725
NlaIII CATG 5 cut(s) 328, 826, 1537, 1630, 1775
NlaIV GGNNCC 4 cut(s) 74, 384, 738, 1235
NmeAIII GCCGAG 1 cut(s) 1757
NmuCI GTSAC 3 cut(s) 23, 128, 880
NsiI ATGCAT 1 cut(s) 457
NspI RCATGY 3 cut(s) 1537, 1630, 1775
PaeI GCATGC 2 cut(s) 1537, 1775
PaeR7I CTCGAG 2 cut(s) 1399, 1417
PceI AGGCCT 1 cut(s) 1441
PctI GAATGC 1 cut(s) 631
PdmI GAANNNNTTC 2 cut(s) 52, 1316
PfeI GAWTC 5 cut(s) 783, 956, 1363, 1619, 1841
PfoI TCCNGGA 1 cut(s) 1236
PleI GAGTC 5 cut(s) 125, 140, 488, 583, 1405
PpsI GAGTC 5 cut(s) 125, 140, 488, 583, 1405
PpuMI RGGWCCY 2 cut(s) 382, 803
PsiI TTATAA 1 cut(s) 1331
Psp1406I AACGTT 1 cut(s) 1143
Psp5II RGGWCCY 2 cut(s) 382, 803
Psp6I CCWGG 5 cut(s) 546, 850, 1236, 1253, 1441
PspFI CCCAGC 2 cut(s) 59, 1694
PspGI CCWGG 5 cut(s) 546, 850, 1236, 1253, 1441
PspN4I GGNNCC 4 cut(s) 74, 384, 738, 1235
PspPI GGNCC 5 cut(s) 73, 212, 382, 803, 1682
PspPPI RGGWCCY 2 cut(s) 382, 803
PstI CTGCAG 1 cut(s) 1189
PstNI CAGNNNCTG 1 cut(s) 1094
PsuI RGATCY 1 cut(s) 200
RsaI GTAC 2 cut(s) 863, 1676
RsaNI GTAC 2 cut(s) 862, 1675
SaqAI TTAA 5 cut(s) 515, 755, 993, 1140, 1791
Sau3AI GATC 9 cut(s) 157, 200, 217, 338, 502, 1221, 1390, 1698, 1725
Sau96I GGNCC 5 cut(s) 73, 212, 382, 803, 1682
SchI GAGTC 5 cut(s) 125, 140, 488, 583, 1405
ScrFI CCNGG 5 cut(s) 548, 852, 1238, 1255, 1443
SduI GDGCHC 2 cut(s) 409, 1600
SfaNI GCATC 7 cut(s) 223, 442, 577, 1171, 1259, 1423, 1806
SfcI CTRYAG 2 cut(s) 811, 1185
Sfr274I CTCGAG 2 cut(s) 1399, 1417
SinI GGWCC 4 cut(s) 73, 382, 803, 1682
SlaI CTCGAG 2 cut(s) 1399, 1417
SmlI CTYRAG 5 cut(s) 32, 180, 992, 1399, 1417
SmoI CTYRAG 5 cut(s) 32, 180, 992, 1399, 1417
SpeI ACTAGT 1 cut(s) 50
SphI GCATGC 2 cut(s) 1537, 1775
Sse9I AATT 9 cut(s) 14, 332, 464, 638, 898, 1241, 1324, 1423, 1636
SseBI AGGCCT 1 cut(s) 1441
SsiI CCGC 8 cut(s) 39, 70, 312, 402, 440, 629, 965, 1373
SspMI CTAG 2 cut(s) 51, 90
StuI AGGCCT 1 cut(s) 1441
StyD4I CCNGG 5 cut(s) 546, 850, 1236, 1253, 1441
StyI CCWWGG 1 cut(s) 707
TaaI ACNGT 5 cut(s) 987, 1096, 1164, 1679, 1738
TaiI ACGT 4 cut(s) 103, 481, 837, 1146
TaqI TCGA 8 cut(s) 295, 1067, 1320, 1400, 1418, 1641, 1728, 1747
TasI AATT 9 cut(s) 14, 332, 464, 638, 898, 1241, 1324, 1423, 1636
TatI WGTACW 1 cut(s) 861
TauI GCSGC 2 cut(s) 41, 443
TfiI GAWTC 5 cut(s) 783, 956, 1363, 1619, 1841
Tru1I TTAA 5 cut(s) 515, 755, 993, 1140, 1791
Tru9I TTAA 5 cut(s) 515, 755, 993, 1140, 1791
TscAI CASTG 3 cut(s) 919, 992, 1099
TseFI GTSAC 3 cut(s) 23, 128, 880
Tsp45I GTSAC 3 cut(s) 23, 128, 880
TspDTI ATGAA 8 cut(s) 99, 313, 497, 624, 651, 1316, 1376, 1470
TspGWI ACGGA 1 cut(s) 1487
TspRI CASTG 3 cut(s) 919, 992, 1099
Vha464I CTTAAG 1 cut(s) 992
VneI GTGCAC 1 cut(s) 405
VpaK11BI GGWCC 4 cut(s) 73, 382, 803, 1682
XagI CCTNNNNNAGG 2 cut(s) 32, 1446
XapI RAATTY 2 cut(s) 638, 1324
XbaI TCTAGA 1 cut(s) 89
XceI RCATGY 3 cut(s) 1537, 1630, 1775
XcmI CCANNNNNNNNNTGG 1 cut(s) 937
XhoI CTCGAG 2 cut(s) 1399, 1417
XmiI GTMKAC 1 cut(s) 810
XmnI GAANNNNTTC 2 cut(s) 52, 1316
XspI CTAG 2 cut(s) 51, 90
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.