Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004782

Physical Location & Seq

Genomic Coordinates Reverse (-)

15796075 .. 15806887

Gene Structure 10813 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1098400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3402 bp

ATGAGTTTGAATTCGTCTCGCTCCGCCGGCTCGTCCGGCCAGCGATCCAATTCCAAGCGGAGCTTCAATTCCAACTCTTCGAACAGTAACTCCAAGAAGAAGCAGCAGCAGACGGCGGCCAACCAGAAAACCCTAGGCGCCTCTTGGGGCGCCAATTCTCTCTCCTCCTCTCGCTCCTCCTTCAAAAAAGCTCCCTTCTCCGATTTTGGCAGTTACATGGTAGAGAAGAACAGGAAACTTCATAATCAGTTCGGTACAGAAGCTTCAGCTTCTTCACACAGTGGTTCAAATACCGGAAATGATATTTTTCGCGGGGTTTCGATATTTGTAGATGGCTACACGGTTCCTCCCAATCAGGAACTGCGAGGCTACATGTTGCACTTCGGTGGACAATATGAGAATTATTTTTCCAGACATCGTGTCACACATATCATTTGCAGTAATCTTCCTGACAGCAAAATAAAAAATTTAAGGTCTTTTAGTGGAGGGCTCCCAGTGGTGAAACCCTCCTGGATATTAGATTCAGTTTCTGCTAACAAACTCTTGAGTTGGGTTCCTTACCAACTTGAACAGATTGCTTGCAACCAACCATTGCTGTCAGCCTTCTTTTCCCCAAAAGTAATCCCAGACTCTGGCGATCCATTGAGAGATGCTGCTAATCAAGTAACATATGAAAGTGAGGTTACATCGCTGGTGGGAGCCATAGTGGAAGCTGCTACCGGATCTGATGTGGGCGGATCCACAGAACATAGAGGGAAAATTAGTGGAGAATCTGATAACATTATGTTTGAGAACAACAATGGAGAACCTGGTCAGGTGCCATATGTAAGTAGTGAAAAAGATTGTGAAGTGCAAATTGCAGATATGACTACTTTGGACTCAGAAGAGCATGACAGTGTAAAAGATGAACTTCAGTGTAGTACCCACCAAACATCAGCTTCCGATTGCAGCTACCACTTGCCAAGCTCTTCAAATGTCGGGTCTAGTAGAAGTCATTCAACTCTTGGGGATTCCAATTTCGTGGAAAACTATTTTAAGAGCTCAAGGCTGCACTTCATAGGAACATGGAGAAATCGGTATCGTAAACGTTTTCCAAGTTCCTTTAAAGGGTTTAAGAACACAGAGCCTAATCTTAGTGCTTCGACGAGTTTTCAGAAGGCTGCCATTATACATATTGACATGGACTGTTTTTTCGTGTCAGTGGTCATCAGGAAACGTCCTGAGTTTAAGGATAGGCCTGTAGCTGTATGCCATTCTGATAATCCAAAGGGAACTGCTGAAATTTCATCTGCAAACTACCCTGCTCGAAATTATGGAGTGAAGGCTGGGATGTTTGTTAGAGATGCCAAGGCTATTTGTCCAAACCTTGTCATTATTCCATATGATTTTGAAGCTTATGAGGAGGTAGCTGATCAGTTCTATGACATCTTGCATAAGCACTGCAATAAAGTACAGGCAGTAAGCTGCGATGAAGCATTTTTAGATGTCACAGATGTGGAAGGGGTAGAGCCTGAGGTGTTAGCTTCAATTGTAAGAAAAGAGATCTTTGAGACAACTGGATGCTCTGCAAGTGCTGGCATAGCCATAAATATGCTTATGGCTCGCCTTGCCACTAAAACTGCTAAGCCAGATGGTCAATGCTATATTCCTACTGAAAAGGTGGATGATTATTTACATCAACTTCCTATCAAGGAACTTCCAGGAATAGGACACACTTTAGAGCAGAAGTTGAAGAAACAAAATGTTCAGACGTGTGGACAGTTGCGTATGATTTCAAAGGACTCTCTTCAAAAGAACTTTGGGATGAAAACTGGGGAGATGTTGTGGAATCATAGTAGGGGCATTGATAACCGGCTAGTTGGGGTGATTCAGGATAGCAAGTCTATTGGTGCTGAAGTGAATTGGGGCGTGAGGTTCAAGGATTTGAAAGATAGTCAACACTTCCTCTCAAGTCTCTGCAAAGAGGTGTCTTTACGCTTGCAAGGATGTGGGGTGCTGGGCCGTACTTTTACCCTTAAGATTAAAAAAAGAAGTAAAGATGCAGGGGAGCCAGTAAAGTATATGGGCTGCGGAGAATGTGAAAACTTGAGCCACTCTGTAACGGTTCCAGCTGCTACTGATGATGTGGAAGTTCTTCAAAGAATAGCAAAGAAGCTCTTTGGGTCTTTCTCTATAGATGTTAAGGAGATCCGTGGTATTGGCTTGCAAGTTTCCAAGCTTGAGAGCACTGACACTTCAAAGCAAGGTGCAGGATTTGGCATAAAATCTTTAAAGTCGTGGCTCACATCAGCCTCAAGGAGTACAGAAAAGAAATCTAATTTTCATTCTATATCTAGGGTGAAGGTGAATATAGATTGTGAGGATCGGAGAAGTGGTGGAACTTCAGGCCCAGTTGGCAACGATTCACATGGTATTCAAACTCCAGTGCTCAATAGTCTGACTAGTGGTGAACCTTCTCTGGACCAGGTTTCTGAACCACCACCTTTATGCCATCTTGATTTGGGAGTTATTGAGAGTCTTCCTCCTGAATTATTTACGGAACTAAATGGAATCTATGGTGGGAAGTTAGTTGATTATGTTGCTAAAAATAAACGAGAAAATTCCAGTGCTACTGTACCACATGAACAAGTACACGGTGCAAGGATTGGGGTTGAGAGACCACTTCTTAATGATGTGGTCCCCAAGAACGAAACATCAGTAGAAATCAGGCATACAATAGTAGAAAAAGAAGCAATTCCTTCTGCTGTGGTGGGAGGGAACCATCATGTGGAAGTTTCTACATCAGGTCTTGGAAACACTGATATAATGCCGTCATCTTTAAGTCAAATAGATACCTTAGTGTTGCAACAGTTGCCTCAAGAACTGAGAGTTGACATACTCGGGCAGCTTCCTGCACACAGGAAAAATGACGTATCTTCAAGTGCTTCCTTGGGCCCTCCTGCAGAAAAGCCTCTGGAATTATTAGGTCCGAGTAACAAGGATCATTCGGGACCAAGTGATCCTGCTTTGAACCACAAACTTTGGATTGGAAGTCCTCCTGGGTGGGTTGATGAGTTCAAATACAGCAAGTGCATGGTGTTAAATGTTTTTGCTGAGTTGTATTACAAATCAGGGTCAAGTCAAAATTTATCCACCATTCTGCGAAATACTATTTTGGAATCTCAACATCCTCCGGATCCAAGTTCTGACAGCTGGATTGAAGCTGTATACAGCTTCTCTGAGCTTCTCAGGGAGTATATTAACTTAAAGATTGACTCAGATATTGAAGAGATCTATGTTTGTTTTCGTCTTCTTAGAAGGTTTACAATGAAATCAAAATTGTTCTTACAAGTATACAACAATGTCTTTCCTTACCTTCAGACATCTGTCGCTGAGAGTTATGGAGGAAACTTGCACATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000002 GO:0000731 GO:0002200 GO:0002206 GO:0002376 GO:0002377 GO:0002440 GO:0002520 GO:0002565 GO:0003674 GO:0003824 GO:0003887 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006281 GO:0006301 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007005 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010224 GO:0016043 GO:0016444 GO:0016445 GO:0016740 GO:0016772 GO:0016779 GO:0017125 GO:0018130 GO:0019438 GO:0019985 GO:0031974 GO:0031981 GO:0032042 GO:0032501 GO:0032502 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0035822 GO:0035861 GO:0042276 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043504 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048731 GO:0048856 GO:0050896 GO:0051716 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0090304 GO:0090734 GO:0140097 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03460 map03460

Pfam Domains

Protein Families

BRCT_2 IMS IMS_C IMS_HHH LIG3_BRCT

Protein Analysis

1134

Amino Acids

124.61

Weight (kDa)

6.73

Isoelectric Point (pI)

44.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LIG3_BRCT	PF16759	100 - 177	6.6e-07	DNA ligase 3 BRCT domain
BRCT	PF00533	101 - 175	9.5e-08	BRCA1 C Terminus (BRCT) domain
BRCT_2	PF16589	102 - 187	4.3e-08	BRCT domain
IMS	PF00817	392 - 539	6.2e-45	impB/mucB/samB family
POLH-Rev1_HhH	PF21704	561 - 615	1.9e-06	DNApol eta/Rev1, HhH motif
IMS_HHH_1	PF21999	564 - 616	3.4e-21	DNA polymerase-iota, thumb domain
IMS_C	PF11799	626 - 748	1.9e-20	impB/mucB/samB family C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004782)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44750 AT5G44750
fragaria_vesca	FvH4_1g23540 FvH4_2g04972
malus_domestica	MD05G1091100.v1.1 MD10G1098400.v1.1
prunus_persica	Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1
pyrus_communis	pycom10g08430
rosa_chinensis	RchiOBHm_Chr6g0254741
rosa_laevigata	RLG00000014893
rosa_multiflora	Rmu_sc0007716.1_g000003
rosa_roxburghii	Rroxscaffold_7G00210560
rosa_rugosa	Rorug05G0554600
rosa_samantha	Rh6AG071600 Rh6BG063800 Rh6CG063200 Rh6DG060300
rosa_wichuraiana	Rw6G006270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	137, 149, 817
AccB7I	CCANNNNNTGG	2 cut(s)	632, 2767
AccI	GTMKAC	2 cut(s)	3207, 3333
AccII	CGCG	1 cut(s)	312
AccIII	TCCGGA	1 cut(s)	3172
AciI	CCGC	6 cut(s)	24, 58, 116, 312, 735, 2070
AclI	AACGTT	1 cut(s)	1087
AcoI	YGGCCR	2 cut(s)	37, 117
AcsI	RAATTY	5 cut(s)	10, 466, 1281, 2599, 3124
AcuI	CTGAAG	5 cut(s)	249, 896, 1914, 2367, 3341
AcyI	GRCGYC	2 cut(s)	138, 150
AdeI	CACNNNGTG	1 cut(s)	281
AfaI	GTAC	7 cut(s)	256, 922, 1452, 2005, 2302, 2616, 2631
AfiI	CCNNNNNNNGG	4 cut(s)	632, 1107, 1706, 2767
AflII	CTTAAG	1 cut(s)	2015
AflIII	ACRYGT	2 cut(s)	372, 1751
AhdI	GACNNNNNGTC	1 cut(s)	419
AhlI	ACTAGT	1 cut(s)	2441
AjiI	CACGTC	1 cut(s)	1752
AjnI	CCWGG	5 cut(s)	509, 808, 1699, 2463, 3037
AjuI	GAANNNNNNNTTGG	2 cut(s)	47, 79
AleI	CACNNNNGTG	2 cut(s)	384, 1493
AloI	GAACNNNNNNTCC	2 cut(s)	74, 106
Alw21I	GWGCWC	3 cut(s)	1043, 2228, 2430
Alw26I	GTCTC	4 cut(s)	21, 1544, 1958, 2650
AlwNI	CAGNNNCTG	3 cut(s)	361, 530, 632
Ama87I	CYCGRG	1 cut(s)	2879
Aor13HI	TCCGGA	1 cut(s)	3172
AoxI	GGCC	6 cut(s)	37, 117, 1235, 1999, 2386, 2932
ApaI	GGGCCC	1 cut(s)	2936
ApoI	RAATTY	5 cut(s)	10, 466, 1281, 2599, 3124
ArsI	GACNNNNNNTTYG	4 cut(s)	965, 997, 1175, 1207
Asp700I	GAANNNNTTC	3 cut(s)	994, 2133, 2733
AspA2I	CCTAGG	1 cut(s)	133
AspLEI	GCGC	2 cut(s)	140, 152
AspS9I	GGNCC	8 cut(s)	1999, 2387, 2461, 2677, 2932, 2933, 2966, 2990
AsuHPI	GGTGA	5 cut(s)	511, 1876, 2350, 2356, 2459
AsuII	TTCGAA	1 cut(s)	80
AvaI	CYCGRG	1 cut(s)	2879
AvaII	GGWCC	4 cut(s)	2461, 2677, 2966, 2990
AvrII	CCTAGG	1 cut(s)	133
AxyI	CCTNAGG	1 cut(s)	1512
BaeGI	GKGCMC	1 cut(s)	2936
BaeI	ACNNNNGTAYC	2 cut(s)	2823, 2856
BamHI	GGATCC	2 cut(s)	737, 3175
BanI	GGYRCC	3 cut(s)	137, 149, 817
BanII	GRGCYC	3 cut(s)	492, 1043, 2936
BarI	GAAGNNNNNNTAC	2 cut(s)	247, 279
BbsI	GAAGAC	2 cut(s)	2510, 3281
Bbv12I	GWGCWC	3 cut(s)	1043, 2228, 2430
BccI	CCATC	4 cut(s)	326, 1625, 2499, 2769
BceAI	ACGGC	3 cut(s)	129, 1986, 2794
BciT130I	CCWGG	5 cut(s)	511, 810, 1701, 2465, 3039
BclI	TGATCA	1 cut(s)	1411
BcoDI	GTCTC	4 cut(s)	21, 1544, 1958, 2650
BcuI	ACTAGT	1 cut(s)	2441
BfaI	CTAG	5 cut(s)	134, 984, 1856, 2334, 2442
BfmI	CTRYAG	3 cut(s)	1239, 2172, 2940
BfoI	RGCGCY	2 cut(s)	141, 153
BfrI	CTTAAG	1 cut(s)	2015
BglI	GCCNNNNNGGC	1 cut(s)	2394
BglII	AGATCT	2 cut(s)	1542, 3270
BlnI	CCTAGG	1 cut(s)	133
BlpI	GCTNAGC	1 cut(s)	1623
Bme1390I	CCNGG	5 cut(s)	511, 810, 1701, 2465, 3039
Bme18I	GGWCC	4 cut(s)	2461, 2677, 2966, 2990
BmeRI	GACNNNNNGTC	1 cut(s)	419
BmeT110I	CYCGRG	1 cut(s)	2879
BmgBI	CACGTC	1 cut(s)	1752
BmgT120I	GGNCC	8 cut(s)	1999, 2387, 2461, 2677, 2932, 2933, 2966, 2990
BmrFI	CCNGG	5 cut(s)	511, 810, 1701, 2465, 3039
BmrI	ACTGGG	3 cut(s)	488, 1821, 2384
BmsI	GCATC	4 cut(s)	640, 1333, 1550, 2029
BmuI	ACTGGG	3 cut(s)	488, 1821, 2384
BoxI	GACNNNNGTC	1 cut(s)	3365
BpiI	GAAGAC	2 cut(s)	2510, 3281
BplI	GAGNNNNNCTC	2 cut(s)	2506, 2538
BpmI	CTGGAG	1 cut(s)	2406
Bpu1102I	GCTNAGC	1 cut(s)	1623
Bpu14I	TTCGAA	1 cut(s)	80
BpuEI	CTTGAG	7 cut(s)	565, 1027, 1933, 2107, 2240, 2278, 2841
BsaHI	GRCGYC	2 cut(s)	138, 150
BsaI	GGTCTC	1 cut(s)	2650
BsaJI	CCNNGG	5 cut(s)	133, 1347, 2191, 2928, 3038
BsaWI	WCCGGW	3 cut(s)	293, 719, 3172
Bsc4I	CCNNNNNNNGG	4 cut(s)	632, 1107, 1706, 2767
Bse118I	RCCGGY	2 cut(s)	26, 1851
Bse1I	ACTGG	7 cut(s)	494, 1561, 1816, 2051, 2390, 2423, 2604
Bse21I	CCTNAGG	1 cut(s)	1512
Bse3DI	GCAATG	1 cut(s)	590
BseAI	TCCGGA	1 cut(s)	3172
BseBI	CCWGG	5 cut(s)	511, 810, 1701, 2465, 3039
BseDI	CCNNGG	5 cut(s)	133, 1347, 2191, 2928, 3038
BseGI	GGATG	6 cut(s)	1335, 1565, 1669, 1809, 1991, 3166
BseLI	CCNNNNNNNGG	4 cut(s)	632, 1107, 1706, 2767
BseMI	GCAATG	1 cut(s)	590
BseMII	CTCAG	9 cut(s)	894, 1212, 1503, 2855, 3084, 3210, 3241, 3270, 3363
BseNI	ACTGG	7 cut(s)	494, 1561, 1816, 2051, 2390, 2423, 2604
BseRI	GAGGAG	4 cut(s)	154, 157, 166, 1415
BseSI	GKGCMC	1 cut(s)	2936
BseYI	CCCAGC	2 cut(s)	1325, 1996
BsgI	GTGCAG	3 cut(s)	1034, 2268, 2877
Bsh1236I	CGCG	1 cut(s)	312
BshFI	GGCC	6 cut(s)	39, 119, 1237, 2001, 2388, 2934
BshNI	GGYRCC	3 cut(s)	137, 149, 817
BsiHKAI	GWGCWC	3 cut(s)	1043, 2228, 2430
BsiHKCI	CYCGRG	1 cut(s)	2879
BsiSI	CCGG	6 cut(s)	27, 36, 294, 720, 1852, 3173
BslFI	GGGAC	2 cut(s)	2663, 3003
BslI	CCNNNNNNNGG	4 cut(s)	632, 1107, 1706, 2767
BsmAI	GTCTC	4 cut(s)	21, 1544, 1958, 2650
BsmBI	CGTCTC	1 cut(s)	21
BsmFI	GGGAC	2 cut(s)	2663, 3003
BsnI	GGCC	6 cut(s)	39, 119, 1237, 2001, 2388, 2934
Bso31I	GGTCTC	1 cut(s)	2650
BsoBI	CYCGRG	1 cut(s)	2879
Bsp119I	TTCGAA	1 cut(s)	80
Bsp120I	GGGCCC	1 cut(s)	2932
Bsp1286I	GDGCHC	5 cut(s)	492, 1043, 2228, 2430, 2936
Bsp13I	TCCGGA	1 cut(s)	3172
Bsp1720I	GCTNAGC	1 cut(s)	1623
BspACI	CCGC	6 cut(s)	24, 58, 116, 312, 735, 2070
BspANI	GGCC	6 cut(s)	39, 119, 1237, 2001, 2388, 2934
BspCNI	CTCAG	9 cut(s)	893, 1213, 1504, 2856, 3085, 3211, 3240, 3269, 3364
BspEI	TCCGGA	1 cut(s)	3172
BspFNI	CGCG	1 cut(s)	312
BspMAI	CTGCAG	1 cut(s)	2944
BspQI	GCTCTTC	2 cut(s)	879, 973
BspT104I	TTCGAA	1 cut(s)	80
BspT107I	GGYRCC	3 cut(s)	137, 149, 817
BspTI	CTTAAG	1 cut(s)	2015
BspTNI	GGTCTC	1 cut(s)	2650
BsrDI	GCAATG	1 cut(s)	590
BsrFI	RCCGGY	2 cut(s)	26, 1851
BsrI	ACTGG	7 cut(s)	494, 1561, 1816, 2051, 2390, 2423, 2604
BssAI	RCCGGY	2 cut(s)	26, 1851
BssECI	CCNNGG	5 cut(s)	133, 1347, 2191, 2928, 3038
BssNAI	GTATAC	2 cut(s)	3208, 3334
BssNI	GRCGYC	2 cut(s)	138, 150
BssT1I	CCWWGG	3 cut(s)	133, 1347, 2928
Bst1107I	GTATAC	2 cut(s)	3208, 3334
Bst2UI	CCWGG	5 cut(s)	511, 810, 1701, 2465, 3039
Bst6I	CTCTTC	5 cut(s)	82, 879, 973, 1791, 3261
BstACI	GRCGYC	2 cut(s)	138, 150
BstAFI	CTTAAG	1 cut(s)	2015
BstAPI	GCANNNNNTGC	1 cut(s)	2851
BstBI	TTCGAA	1 cut(s)	80
BstC8I	GCNNGC	7 cut(s)	28, 41, 580, 1576, 1603, 1979, 2204
BstDSI	CCRYGG	1 cut(s)	2191
BstF5I	GGATG	6 cut(s)	1335, 1565, 1669, 1809, 1991, 3166
BstFNI	CGCG	1 cut(s)	312
BstH2I	RGCGCY	2 cut(s)	141, 153
BstHHI	GCGC	2 cut(s)	140, 152
BstMAI	GTCTC	4 cut(s)	21, 1544, 1958, 2650
BstMWI	GCNNNNNNNGC	6 cut(s)	27, 36, 1580, 1607, 2394, 2851
BstNI	CCWGG	5 cut(s)	511, 810, 1701, 2465, 3039
BstNSI	RCATGY	1 cut(s)	376
BstPAI	GACNNNNGTC	1 cut(s)	3365
BstSCI	CCNGG	5 cut(s)	509, 808, 1699, 2463, 3037
BstSFI	CTRYAG	3 cut(s)	1239, 2172, 2940
BstSLI	GKGCMC	1 cut(s)	2936
BstUI	CGCG	1 cut(s)	312
BstV2I	GAAGAC	2 cut(s)	2510, 3281
BstX2I	RGATCY	6 cut(s)	722, 737, 1542, 2187, 3175, 3270
BstXI	CCANNNNNNTGG	1 cut(s)	1021
BstYI	RGATCY	6 cut(s)	722, 737, 1542, 2187, 3175, 3270
BstZ17I	GTATAC	2 cut(s)	3208, 3334
Bsu36I	CCTNAGG	1 cut(s)	1512
BsuRI	GGCC	6 cut(s)	39, 119, 1237, 2001, 2388, 2934
BtgI	CCRYGG	1 cut(s)	2191
BtgZI	GCGATG	2 cut(s)	672, 1482
BtrI	CACGTC	1 cut(s)	1752
BtsCI	GGATG	6 cut(s)	1335, 1565, 1669, 1809, 1991, 3166
BtsI	GCAGTG	1 cut(s)	1438
Cac8I	GCNNGC	7 cut(s)	28, 41, 580, 1576, 1603, 1979, 2204
CaiI	CAGNNNCTG	3 cut(s)	361, 530, 632
CfoI	GCGC	2 cut(s)	140, 152
Cfr10I	RCCGGY	2 cut(s)	26, 1851
Cfr13I	GGNCC	8 cut(s)	1999, 2387, 2461, 2677, 2932, 2933, 2966, 2990
CsiI	ACCWGGT	2 cut(s)	808, 2463
Csp6I	GTAC	7 cut(s)	255, 921, 1451, 2004, 2301, 2615, 2630
CspCI	CAANNNNNGTGG	2 cut(s)	3002, 3037
CviAII	CATG	9 cut(s)	217, 373, 890, 1065, 1180, 2408, 2621, 2765, 3073
CviQI	GTAC	7 cut(s)	255, 921, 1451, 2004, 2301, 2615, 2630
DinI	GGCGCC	2 cut(s)	139, 151
DraI	TTTAAA	2 cut(s)	1105, 2271
DraIII	CACNNNGTG	1 cut(s)	281
DriI	GACNNNNNGTC	1 cut(s)	419
EaeI	YGGCCR	2 cut(s)	37, 117
Eam1104I	CTCTTC	5 cut(s)	82, 879, 973, 1791, 3261
Eam1105I	GACNNNNNGTC	1 cut(s)	419
EarI	CTCTTC	5 cut(s)	82, 879, 973, 1791, 3261
EciI	GGCGGA	2 cut(s)	13, 750
Ecl136II	GAGCTC	1 cut(s)	1041
Eco130I	CCWWGG	3 cut(s)	133, 1347, 2928
Eco147I	AGGCCT	1 cut(s)	1237
Eco24I	GRGCYC	3 cut(s)	492, 1043, 2936
Eco31I	GGTCTC	1 cut(s)	2650
Eco47I	GGWCC	4 cut(s)	2461, 2677, 2966, 2990
Eco53kI	GAGCTC	1 cut(s)	1041
Eco57I	CTGAAG	5 cut(s)	249, 896, 1914, 2367, 3341
Eco81I	CCTNAGG	1 cut(s)	1512
Eco88I	CYCGRG	1 cut(s)	2879
EcoICRI	GAGCTC	1 cut(s)	1041
EcoO109I	RGGNCCY	1 cut(s)	2933
EcoRI	GAATTC	1 cut(s)	10
EcoRII	CCWGG	5 cut(s)	509, 808, 1699, 2463, 3037
EcoT14I	CCWWGG	3 cut(s)	133, 1347, 2928
EcoT38I	GRGCYC	3 cut(s)	492, 1043, 2936
EgeI	GGCGCC	2 cut(s)	139, 151
EheI	GGCGCC	2 cut(s)	139, 151
ErhI	CCWWGG	3 cut(s)	133, 1347, 2928
Esp3I	CGTCTC	1 cut(s)	21
FaeI	CATG	9 cut(s)	220, 376, 893, 1068, 1183, 2411, 2624, 2768, 3076
FalI	AAGNNNNNCTT	6 cut(s)	47, 79, 894, 926, 2141, 2173
FaqI	GGGAC	2 cut(s)	2663, 3003
FatI	CATG	9 cut(s)	216, 372, 889, 1064, 1179, 2407, 2620, 2764, 3072
FauI	CCCGC	1 cut(s)	305
FauNDI	CATATG	3 cut(s)	670, 823, 1381
FbaI	TGATCA	1 cut(s)	1411
FblI	GTMKAC	2 cut(s)	3207, 3333
FokI	GGATG	6 cut(s)	1342, 1572, 1676, 1816, 1998, 3153
FriOI	GRGCYC	3 cut(s)	492, 1043, 2936
FspBI	CTAG	5 cut(s)	134, 984, 1856, 2334, 2442
GlaI	GCGC	2 cut(s)	139, 151
GsaI	CCCAGC	2 cut(s)	1329, 2000
GsuI	CTGGAG	1 cut(s)	2406
HaeII	RGCGCY	2 cut(s)	141, 153
HaeIII	GGCC	6 cut(s)	39, 119, 1237, 2001, 2388, 2934
HapII	CCGG	6 cut(s)	27, 36, 294, 720, 1852, 3173
HhaI	GCGC	2 cut(s)	140, 152
Hin1I	GRCGYC	2 cut(s)	138, 150
Hin1II	CATG	9 cut(s)	220, 376, 893, 1068, 1183, 2411, 2624, 2768, 3076
Hin6I	GCGC	2 cut(s)	138, 150
HinP1I	GCGC	2 cut(s)	138, 150
HincII	GTYRAC	2 cut(s)	1937, 2872
HindII	GTYRAC	2 cut(s)	1937, 2872
HindIII	AAGCTT	3 cut(s)	261, 1392, 2216
HpaII	CCGG	6 cut(s)	27, 36, 294, 720, 1852, 3173
HphI	GGTGA	5 cut(s)	511, 1876, 2350, 2356, 2459
Hpy99I	CGWCG	1 cut(s)	1147
HpyCH4IV	ACGT	4 cut(s)	1087, 1216, 1751, 2910
HpyF10VI	GCNNNNNNNGC	6 cut(s)	27, 36, 1580, 1607, 2394, 2851
HpySE526I	ACGT	4 cut(s)	1087, 1216, 1751, 2910
Hsp92I	GRCGYC	2 cut(s)	138, 150
Hsp92II	CATG	9 cut(s)	220, 376, 893, 1068, 1183, 2411, 2624, 2768, 3076
HspAI	GCGC	2 cut(s)	138, 150
KasI	GGCGCC	2 cut(s)	137, 149
Kpn2I	TCCGGA	1 cut(s)	3172
KroI	GCCGGC	1 cut(s)	26
KroNI	GCCGGC	1 cut(s)	28
Ksp22I	TGATCA	1 cut(s)	1411
LguI	GCTCTTC	2 cut(s)	879, 973
LmnI	GCTCC	7 cut(s)	26, 60, 179, 196, 495, 698, 2047
LweI	GCATC	4 cut(s)	640, 1333, 1550, 2029
MabI	ACCWGGT	2 cut(s)	808, 2463
MaeI	CTAG	5 cut(s)	134, 984, 1856, 2334, 2442
MaeII	ACGT	4 cut(s)	1087, 1216, 1751, 2910
MaeIII	GTNAC	8 cut(s)	86, 212, 421, 664, 682, 1486, 2098, 2972
MfeI	CAATTG	1 cut(s)	1527
MflI	RGATCY	6 cut(s)	722, 737, 1542, 2187, 3175, 3270
MhlI	GDGCHC	5 cut(s)	492, 1043, 2228, 2430, 2936
Mly113I	GGCGCC	2 cut(s)	138, 150
MlyI	GAGTC	5 cut(s)	623, 872, 1775, 2524, 3248
MmeI	TCCRAC	1 cut(s)	96
MroI	TCCGGA	1 cut(s)	3172
MroNI	GCCGGC	1 cut(s)	26
MroXI	GAANNNNTTC	3 cut(s)	994, 2133, 2733
MslI	CAYNNNNRTG	5 cut(s)	384, 894, 1493, 1589, 2485
MspA1I	CMGCKG	2 cut(s)	2111, 3192
MspCI	CTTAAG	1 cut(s)	2015
MspI	CCGG	6 cut(s)	27, 36, 294, 720, 1852, 3173
MspR9I	CCNGG	5 cut(s)	511, 810, 1701, 2465, 3039
MunI	CAATTG	1 cut(s)	1527
MvaI	CCWGG	5 cut(s)	511, 810, 1701, 2465, 3039
MvnI	CGCG	1 cut(s)	312
MwoI	GCNNNNNNNGC	6 cut(s)	27, 36, 1580, 1607, 2394, 2851
NaeI	GCCGGC	1 cut(s)	28
NarI	GGCGCC	2 cut(s)	138, 150
NdeI	CATATG	3 cut(s)	670, 823, 1381
NgoMIV	GCCGGC	1 cut(s)	26
NlaIII	CATG	9 cut(s)	220, 376, 893, 1068, 1183, 2411, 2624, 2768, 3076
NmuCI	GTSAC	2 cut(s)	421, 1486
NspI	RCATGY	1 cut(s)	376
NspV	TTCGAA	1 cut(s)	80
OliI	CACNNNNGTG	2 cut(s)	384, 1493
PceI	AGGCCT	1 cut(s)	1237
PciI	ACATGT	1 cut(s)	372
PciSI	GCTCTTC	2 cut(s)	879, 973
PdiI	GCCGGC	1 cut(s)	28
PdmI	GAANNNNTTC	3 cut(s)	994, 2133, 2733
PfeI	GAWTC	8 cut(s)	521, 770, 1010, 1828, 1867, 2402, 2550, 3158
PflMI	CCANNNNNTGG	2 cut(s)	632, 2767
PfoI	TCCNGGA	2 cut(s)	509, 1699
PleI	GAGTC	5 cut(s)	623, 872, 1775, 2523, 3248
PluTI	GGCGCC	2 cut(s)	141, 153
PpsI	GAGTC	5 cut(s)	623, 872, 1775, 2523, 3248
PscI	ACATGT	1 cut(s)	372
PshAI	GACNNNNGTC	1 cut(s)	3365
Psp124BI	GAGCTC	1 cut(s)	1043
Psp1406I	AACGTT	1 cut(s)	1087
Psp6I	CCWGG	5 cut(s)	509, 808, 1699, 2463, 3037
PspFI	CCCAGC	2 cut(s)	1325, 1996
PspGI	CCWGG	5 cut(s)	509, 808, 1699, 2463, 3037
PspOMI	GGGCCC	1 cut(s)	2932
PspPI	GGNCC	8 cut(s)	1999, 2387, 2461, 2677, 2932, 2933, 2966, 2990
PstI	CTGCAG	1 cut(s)	2944
PstNI	CAGNNNCTG	3 cut(s)	361, 530, 632
PsuI	RGATCY	6 cut(s)	722, 737, 1542, 2187, 3175, 3270
PvuII	CAGCTG	2 cut(s)	2111, 3192
RsaI	GTAC	7 cut(s)	256, 922, 1452, 2005, 2302, 2616, 2631
RsaNI	GTAC	7 cut(s)	255, 921, 1451, 2004, 2301, 2615, 2630
RseI	CAYNNNNRTG	5 cut(s)	384, 894, 1493, 1589, 2485
SacI	GAGCTC	1 cut(s)	1043
SapI	GCTCTTC	2 cut(s)	879, 973
Sau96I	GGNCC	8 cut(s)	1999, 2387, 2461, 2677, 2932, 2933, 2966, 2990
SchI	GAGTC	5 cut(s)	623, 872, 1775, 2524, 3248
ScrFI	CCNGG	5 cut(s)	511, 810, 1701, 2465, 3039
SduI	GDGCHC	5 cut(s)	492, 1043, 2228, 2430, 2936
SexAI	ACCWGGT	2 cut(s)	808, 2463
SfaNI	GCATC	4 cut(s)	640, 1333, 1550, 2029
SfcI	CTRYAG	3 cut(s)	1239, 2172, 2940
SfoI	GGCGCC	2 cut(s)	139, 151
SfuI	TTCGAA	1 cut(s)	80
SinI	GGWCC	4 cut(s)	2461, 2677, 2966, 2990
SmiMI	CAYNNNNRTG	5 cut(s)	384, 894, 1493, 1589, 2485
SmlI	CTYRAG	8 cut(s)	544, 1042, 1948, 2015, 2086, 2219, 2293, 2856
SmoI	CTYRAG	8 cut(s)	544, 1042, 1948, 2015, 2086, 2219, 2293, 2856
SpeI	ACTAGT	1 cut(s)	2441
SseBI	AGGCCT	1 cut(s)	1237
SsiI	CCGC	6 cut(s)	24, 58, 116, 312, 735, 2070
SspDI	GGCGCC	2 cut(s)	137, 149
SspMI	CTAG	5 cut(s)	134, 984, 1856, 2334, 2442
SstI	GAGCTC	1 cut(s)	1043
StuI	AGGCCT	1 cut(s)	1237
StyD4I	CCNGG	5 cut(s)	509, 808, 1699, 2463, 3037
StyI	CCWWGG	3 cut(s)	133, 1347, 2928
TaiI	ACGT	4 cut(s)	1090, 1219, 1754, 2913
TaqI	TCGA	4 cut(s)	80, 320, 1142, 1306
TatI	WGTACW	3 cut(s)	1450, 2300, 2629
TauI	GCSGC	1 cut(s)	119
TfiI	GAWTC	8 cut(s)	521, 770, 1010, 1828, 1867, 2402, 2550, 3158
TseFI	GTSAC	2 cut(s)	421, 1486
Tsp45I	GTSAC	2 cut(s)	421, 1486
TspGWI	ACGGA	2 cut(s)	2180, 2552
Van91I	CCANNNNNTGG	2 cut(s)	632, 2767
Vha464I	CTTAAG	1 cut(s)	2015
VpaK11BI	GGWCC	4 cut(s)	2461, 2677, 2966, 2990
XapI	RAATTY	5 cut(s)	10, 466, 1281, 2599, 3124
XceI	RCATGY	1 cut(s)	376
XmaJI	CCTAGG	1 cut(s)	133
XmiI	GTMKAC	2 cut(s)	3207, 3333
XmnI	GAANNNNTTC	3 cut(s)	994, 2133, 2733
XspI	CTAG	5 cut(s)	134, 984, 1856, 2334, 2442

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.