Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0004782

Physical Location & Seq

Genomic Coordinates Reverse (-)

59186992 .. 59195238

Gene Structure 8247 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000014893

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2610 bp

ATGAGTTTGAATTCGTCAAAATCGTCTGGTTCAAAATCCAAGCGAAGCTTCAATTCCAACAATTCCACTAACAACAACACCAAGAAGACGAAGATTAGCACCAACCAGAAAACCCTAGGCGGCGTCTCTTGGGGCGCCAATTCTATCTCCTCCTCTCGCTCCTCCTTCCAAAGATCCCCCTTTCCCGATTTCGGCAGTTATATGGTAGAGAAGAACAGGAAACTGCAGAATCAGTTTGATAGTGAAGCTTCGAGTTGTTTGGGGTCCGAGAGGAGTATTTTTCGTGGGGTTTCGATTTTCGTAGACGGTTTCACAGTGCCTTCTAGTCAGGAACTGAGAGCGTACATGGTGAATTATGGTGGGAGATATGAGAATTACTTTTCAAGGCGTTGTGTTACACATATCATCTGCAGTAATCTTCCTGACAGTAAAATAAAAAATCTGAGGTCTTTCAGTGGAGGGCTCCCAGTTGTGAAGCCTAACTGGATTGTAGATTCAGTTGCTGCTAACAAACTCTTGAGTTGGGTTCCTTACCAACTTGAACAGGTTTCTTGTAACCAACCAAGGTTGTCAGCCTTCTTTGCTCCGAAGATAATCCCCAACTGTGATGATGCTTTGAGAGATACATCTGATCAAGTGAAACCTGAAAGTGAGGATACATCTTCGGTAGGAACCAGATTAGAAGATGATAACAAGTCAGCACGCAGATCCACTGAACACGAACGGGAAAGTAGCGGAGAATCTGATGATATGATATATGAAAACACCGATGGACAATTTGGTGAGGAGTTGTATACAGGTGAAAAATATTCTGAAGTTAAACAAGAAGAAACGCCTACTTCAGATGCAGAAGATAATGTCAGCATCAAGGATGAAGTTAAATCTAGTACCCACCAGCACTCTGCTTCAGTTAGCAGTAACTGCTTGCCAAGCTCAGAAAATTTTGGGTCTAATCGAAGTCATTCAACTCTTGGGGATCCCAATTTTGTGGAAAATTATTTTAAGAGCTCAAGGCTGCACTTTATAGGAACCTGGAGAAATCGGTATCGCAAGCGTTTTCCTAGTTCGTCTAAAGGGTTGGACAATACAGATTCTAACCGTTCTGCTCCTGATAGTTTTCCGAAGACTCCCATTATACATATTGACATGGACTGTTTTTTTGTGTCCGTGGTCATCAGGCAACGCCCTGAACTTAAGGATAGGCCTGTAGCTGTATGTCATTCTGATAATCCGAAGGGCACTGCTGAAATTTCATCTGCTAACTACCCTGCTCGAGATTATGGAGTGAGGGCTGGGATGTTTGTCAGAGATGCCAAGGCACTTTGTCCACACCTTGTCATTCTTCCTTATGATTTTGAAGCGTATGAGGAGGTAGCTGATCAGTTCTATGACATTCTGCATAAGCACTGCAGAAAAGTACAAGCAGTAAGCTGTGATGAAGCATTTTTAGATGTCACATACTTGGAAGGGGTAGATATGGATATGTTAGCCTCAACTGTAAGACAAGAGATTTTTGAGACTACTGGATGCACTGCAAGTGCCGGCATAGCTAGAAATATGCTGATGGCTCGCCTTGCAACAAGAACTGCTAAACCAGATGGTCAATGCAACATTCCTCCCGAAAGGGTGGATGATTATTTATATGAACTTCCCATCAAGACGCTTCCAGGAATAGGACACGTTCTAGAGGAGAAGTTGAAGAAGCGAAATGTTTTGACGTGTGGACAGTTGCGTATGATTCCTAAGGACACTCTTCAAAAGGACTTCGGAATAAAAACTGGGGAGATGTTGTGGAATCATAGTAGAGGCATAGATAATCGGCTAGTTGGGGTGATACAGGAAAGTAAGTCTATTGGTGCTGAAGTGAATTGGGGTGTGAGATTCAAGGATTTGAAAGATAGTCATCACTTCCTTTCAAATCTTTGCAAGGAGGTTTCTTTACGCTTGCAAGGATGCACAGTGCAGGGCCGTACTTTTACCCTTAAGATTAAGAAAAGAAGAAAAGATGCACAGGAGCCTGTGAAGTATATGGGATGTGGAGACTGTGAAAACTTTAGCCACTCTGTCACGGTTCCAGTTGCTACTGATGATGTGGAAGTGCTTCAGAGAATAACAAAGCAGCTCTTTGGGTTTTTCTCTCTAGATGTCAAGGAGATTCGAGGTATTGGTTTGCAAGTTTCCAAGCTTGAGAGTGTAGATGCTTCAAAGCAAGGACTTGGAAAAAACTCTTTGAAATCGTGGCTCCAATCTGCCAAAGCAAGTACAGAAGAACAATCCAATACTTATTCTGTAGATGATGGGGAGAGGGCGCATGCAGATTGTGAGGGTCGGGGAACTCATCGAACTTCAGGCCAGCTATGCGGAAATTCACTTGGTATTCAAACTCCAGTGGACAATCATCGGTGTAGTGGTGAAACTTCTGCGAACCAGGTTTCAGCACCACCACCTTTATGTCATCTTGATTTAGGAGTTATTGAAAGTCTTCCTCCAGAACTTTTTACAGAATTAAACGGGATATATGGTGGCAAGTTGGTTGATTTTGTTGCTAAAAATAAACGAGAATTCAGTGCAACAACATCTCATGAACGAGTAGATGGTATTTCTTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000002 GO:0000731 GO:0002200 GO:0002206 GO:0002376 GO:0002377 GO:0002440 GO:0002520 GO:0002565 GO:0003674 GO:0003824 GO:0003887 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006281 GO:0006301 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007005 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010224 GO:0016043 GO:0016444 GO:0016445 GO:0016740 GO:0016772 GO:0016779 GO:0017125 GO:0018130 GO:0019438 GO:0019985 GO:0031974 GO:0031981 GO:0032042 GO:0032501 GO:0032502 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0035822 GO:0035861 GO:0042276 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043504 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048731 GO:0048856 GO:0050896 GO:0051716 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0090304 GO:0090734 GO:0140097 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03460 map03460

Pfam Domains

Protein Families

BRCT_2 IMS IMS_C IMS_HHH LIG3_BRCT

Protein Analysis

870

Amino Acids

96.74

Weight (kDa)

6.74

Isoelectric Point (pI)

44.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BRCT	PF00533	91 - 166	1e-09	BRCA1 C Terminus (BRCT) domain
BRCT_2	PF16589	93 - 178	6.2e-07	BRCT domain
LIG3_BRCT	PF16759	93 - 169	2.4e-06	DNA ligase 3 BRCT domain
IMS	PF00817	381 - 528	1.3e-45	impB/mucB/samB family
IMS_HHH	PF11798	540 - 571	9.6e-07	IMS family HHH motif
IMS_HHH_1	PF21999	554 - 605	1.2e-19	DNA polymerase-iota, thumb domain
IMS_C	PF11799	615 - 736	7.1e-19	impB/mucB/samB family C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004782)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44750 AT5G44750
fragaria_vesca	FvH4_1g23540 FvH4_2g04972
malus_domestica	MD05G1091100.v1.1 MD10G1098400.v1.1
prunus_persica	Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1
pyrus_communis	pycom10g08430
rosa_chinensis	RchiOBHm_Chr6g0254741
rosa_laevigata	RLG00000014893
rosa_multiflora	Rmu_sc0007716.1_g000003
rosa_roxburghii	Rroxscaffold_7G00210560
rosa_rugosa	Rorug05G0554600
rosa_samantha	Rh6AG071600 Rh6BG063800 Rh6CG063200 Rh6DG060300
rosa_wichuraiana	Rw6G006270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	134
AccI	GTMKAC	2 cut(s)	303, 794
AciI	CCGC	3 cut(s)	120, 735, 2361
AclWI	GGATC	4 cut(s)	168, 702, 971, 984
AcsI	RAATTY	5 cut(s)	10, 940, 1248, 2365, 2561
AcuI	CTGAAG	6 cut(s)	825, 834, 891, 1881, 2087, 2331
AcyI	GRCGYC	2 cut(s)	123, 135
AfaI	GTAC	5 cut(s)	344, 889, 1419, 1972, 2263
AfiI	CCNNNNNNNGG	2 cut(s)	191, 1673
AflII	CTTAAG	2 cut(s)	1193, 1982
AflIII	ACRYGT	2 cut(s)	1678, 1718
AjiI	CACGTC	1 cut(s)	1719
AjnI	CCWGG	3 cut(s)	1031, 1666, 2427
AjuI	GAANNNNNNNTTGG	2 cut(s)	32, 64
Alw21I	GWGCWC	1 cut(s)	1010
Alw26I	GTCTC	3 cut(s)	130, 1511, 2034
AlwI	GGATC	4 cut(s)	168, 702, 971, 984
AlwNI	CAGNNNCTG	3 cut(s)	334, 503, 921
Ama87I	CYCGRG	1 cut(s)	1272
AoxI	GGCC	3 cut(s)	1202, 1966, 2350
ApeKI	GCWGC	3 cut(s)	503, 1015, 2119
ApoI	RAATTY	5 cut(s)	10, 940, 1248, 2365, 2561
ArsI	GACNNNNNNTTYG	2 cut(s)	1142, 1174
Asp700I	GAANNNNTTC	3 cut(s)	961, 2100, 2481
AspA2I	CCTAGG	1 cut(s)	115
AspLEI	GCGC	2 cut(s)	137, 2311
AspS9I	GGNCC	2 cut(s)	264, 1966
AsuHPI	GGTGA	5 cut(s)	361, 794, 812, 1843, 2423
AvaI	CYCGRG	1 cut(s)	1272
AvaII	GGWCC	1 cut(s)	264
AvrII	CCTAGG	1 cut(s)	115
AxyI	CCTNAGG	1 cut(s)	1743
BaeGI	GKGCMC	1 cut(s)	1241
BamHI	GGATCC	1 cut(s)	976
BanI	GGYRCC	1 cut(s)	134
BanII	GRGCYC	2 cut(s)	465, 1010
BbsI	GAAGAC	3 cut(s)	92, 1130, 2474
Bbv12I	GWGCWC	1 cut(s)	1010
BbvI	GCAGC	3 cut(s)	490, 1002, 2131
BccI	CCATC	6 cut(s)	764, 1558, 1592, 1661, 2291, 2588
BceAI	ACGGC	1 cut(s)	1953
BciT130I	CCWGG	3 cut(s)	1033, 1668, 2429
BciVI	GTATCC	1 cut(s)	649
BclI	TGATCA	2 cut(s)	631, 1378
BcoDI	GTCTC	3 cut(s)	130, 1511, 2034
BfaI	CTAG	9 cut(s)	116, 324, 885, 1062, 1551, 1685, 1823, 2141, 2608
BfmI	CTRYAG	5 cut(s)	224, 409, 1206, 1408, 2289
BfoI	RGCGCY	1 cut(s)	138
BfrI	CTTAAG	2 cut(s)	1193, 1982
BfuI	GTATCC	1 cut(s)	649
BisI	GCNGC	4 cut(s)	121, 504, 1016, 2120
BlnI	CCTAGG	1 cut(s)	115
BlsI	GCNGC	4 cut(s)	122, 505, 1017, 2121
Bme1390I	CCNGG	3 cut(s)	1033, 1668, 2429
Bme18I	GGWCC	1 cut(s)	264
BmeT110I	CYCGRG	1 cut(s)	1272
BmgBI	CACGTC	1 cut(s)	1719
BmgT120I	GGNCC	2 cut(s)	264, 1966
BmrFI	CCNGG	3 cut(s)	1033, 1668, 2429
BmrI	ACTGGG	2 cut(s)	461, 1788
BmsI	GCATC	8 cut(s)	601, 835, 873, 1300, 1517, 1943, 1996, 2188
BmuI	ACTGGG	2 cut(s)	461, 1788
BpiI	GAAGAC	3 cut(s)	92, 1130, 2474
BpmI	CTGGAG	3 cut(s)	1054, 2370, 2472
BpuEI	CTTGAG	3 cut(s)	538, 994, 2207
BsaBI	GATNNNNATC	1 cut(s)	1902
BsaHI	GRCGYC	2 cut(s)	123, 135
BsaJI	CCNNGG	4 cut(s)	115, 563, 1167, 1314
BsaXI	ACNNNNNCTCC	2 cut(s)	1775, 1805
Bsc4I	CCNNNNNNNGG	2 cut(s)	191, 1673
Bse118I	RCCGGY	1 cut(s)	1541
Bse1I	ACTGG	6 cut(s)	467, 488, 1528, 1783, 2075, 2387
Bse21I	CCTNAGG	1 cut(s)	1743
Bse8I	GATNNNNATC	1 cut(s)	1902
BseBI	CCWGG	3 cut(s)	1033, 1668, 2429
BseDI	CCNNGG	4 cut(s)	115, 563, 1167, 1314
BseGI	GGATG	6 cut(s)	877, 1302, 1532, 1636, 1958, 2039
BseJI	GATNNNNATC	1 cut(s)	1902
BseLI	CCNNNNNNNGG	2 cut(s)	191, 1673
BseMII	CTCAG	3 cut(s)	326, 434, 948
BseNI	ACTGG	6 cut(s)	467, 488, 1528, 1783, 2075, 2387
BseRI	GAGGAG	7 cut(s)	139, 142, 151, 286, 800, 1382, 1703
BseSI	GKGCMC	1 cut(s)	1241
BseXI	GCAGC	3 cut(s)	490, 1002, 2131
BseYI	CCCAGC	1 cut(s)	1292
BsgI	GTGCAG	2 cut(s)	1001, 1982
BshFI	GGCC	3 cut(s)	1204, 1968, 2352
BshNI	GGYRCC	1 cut(s)	134
BsiHKAI	GWGCWC	1 cut(s)	1010
BsiHKCI	CYCGRG	1 cut(s)	1272
BsiSI	CCGG	1 cut(s)	1542
BslI	CCNNNNNNNGG	2 cut(s)	191, 1673
BsmAI	GTCTC	3 cut(s)	130, 1511, 2034
BsmBI	CGTCTC	1 cut(s)	130
BsnI	GGCC	3 cut(s)	1204, 1968, 2352
BsoBI	CYCGRG	1 cut(s)	1272
Bsp1286I	GDGCHC	3 cut(s)	465, 1010, 1241
Bsp143I	GATC	5 cut(s)	173, 631, 707, 976, 1378
BspACI	CCGC	3 cut(s)	120, 735, 2361
BspANI	GGCC	3 cut(s)	1204, 1968, 2352
BspCNI	CTCAG	3 cut(s)	327, 435, 947
BspHI	TCATGA	1 cut(s)	2581
BspMAI	CTGCAG	3 cut(s)	228, 413, 1412
BspPI	GGATC	4 cut(s)	168, 702, 971, 984
BspT107I	GGYRCC	1 cut(s)	134
BspTI	CTTAAG	2 cut(s)	1193, 1982
BsrFI	RCCGGY	1 cut(s)	1541
BsrI	ACTGG	6 cut(s)	467, 488, 1528, 1783, 2075, 2387
BssAI	RCCGGY	1 cut(s)	1541
BssECI	CCNNGG	4 cut(s)	115, 563, 1167, 1314
BssMI	GATC	5 cut(s)	173, 631, 707, 976, 1378
BssNAI	GTATAC	1 cut(s)	795
BssNI	GRCGYC	2 cut(s)	123, 135
BssT1I	CCWWGG	3 cut(s)	115, 563, 1314
Bst1107I	GTATAC	1 cut(s)	795
Bst2UI	CCWGG	3 cut(s)	1033, 1668, 2429
Bst6I	CTCTTC	1 cut(s)	1758
BstACI	GRCGYC	2 cut(s)	123, 135
BstAFI	CTTAAG	2 cut(s)	1193, 1982
BstAPI	GCANNNNNTGC	1 cut(s)	921
BstC8I	GCNNGC	8 cut(s)	703, 926, 1052, 1543, 1570, 1946, 2313, 2354
BstDEI	CTNAG	4 cut(s)	335, 443, 934, 1743
BstDSI	CCRYGG	1 cut(s)	1167
BstF5I	GGATG	6 cut(s)	877, 1302, 1532, 1636, 1958, 2039
BstH2I	RGCGCY	1 cut(s)	138
BstHHI	GCGC	2 cut(s)	137, 2311
BstKTI	GATC	5 cut(s)	176, 634, 710, 979, 1381
BstMAI	GTCTC	3 cut(s)	130, 1511, 2034
BstMBI	GATC	5 cut(s)	173, 631, 707, 976, 1378
BstMWI	GCNNNNNNNGC	6 cut(s)	581, 921, 930, 1547, 1574, 2358
BstNI	CCWGG	3 cut(s)	1033, 1668, 2429
BstNSI	RCATGY	1 cut(s)	2315
BstSCI	CCNGG	3 cut(s)	1031, 1666, 2427
BstSFI	CTRYAG	5 cut(s)	224, 409, 1206, 1408, 2289
BstSLI	GKGCMC	1 cut(s)	1241
BstV1I	GCAGC	3 cut(s)	490, 1002, 2131
BstV2I	GAAGAC	3 cut(s)	92, 1130, 2474
BstX2I	RGATCY	3 cut(s)	173, 707, 976
BstXI	CCANNNNNNTGG	1 cut(s)	988
BstYI	RGATCY	3 cut(s)	173, 707, 976
BstZ17I	GTATAC	1 cut(s)	795
Bsu36I	CCTNAGG	1 cut(s)	1743
BsuI	GTATCC	1 cut(s)	649
BsuRI	GGCC	3 cut(s)	1204, 1968, 2352
BtgI	CCRYGG	1 cut(s)	1167
BtrI	CACGTC	1 cut(s)	1719
BtsCI	GGATG	6 cut(s)	877, 1302, 1532, 1636, 1958, 2039
BtsI	GCAGTG	3 cut(s)	1239, 1405, 1530
BtsIMutI	CAGTG	9 cut(s)	321, 460, 711, 1239, 1405, 1530, 1965, 2394, 2572
Cac8I	GCNNGC	8 cut(s)	703, 926, 1052, 1543, 1570, 1946, 2313, 2354
CaiI	CAGNNNCTG	3 cut(s)	334, 503, 921
CciI	TCATGA	1 cut(s)	2581
CfoI	GCGC	2 cut(s)	137, 2311
Cfr10I	RCCGGY	1 cut(s)	1541
Cfr13I	GGNCC	2 cut(s)	264, 1966
CseI	GACGC	2 cut(s)	112, 1669
CsiI	ACCWGGT	1 cut(s)	2427
Csp6I	GTAC	5 cut(s)	343, 888, 1418, 1971, 2262
CviAII	CATG	4 cut(s)	346, 1147, 2312, 2582
CviQI	GTAC	5 cut(s)	343, 888, 1418, 1971, 2262
DdeI	CTNAG	4 cut(s)	335, 443, 934, 1743
DinI	GGCGCC	1 cut(s)	136
DpnI	GATC	5 cut(s)	175, 633, 709, 978, 1380
DpnII	GATC	5 cut(s)	173, 631, 707, 976, 1378
Eam1104I	CTCTTC	1 cut(s)	1758
EarI	CTCTTC	1 cut(s)	1758
Ecl136II	GAGCTC	1 cut(s)	1008
Eco130I	CCWWGG	3 cut(s)	115, 563, 1314
Eco147I	AGGCCT	1 cut(s)	1204
Eco24I	GRGCYC	2 cut(s)	465, 1010
Eco47I	GGWCC	1 cut(s)	264
Eco53kI	GAGCTC	1 cut(s)	1008
Eco57I	CTGAAG	6 cut(s)	825, 834, 891, 1881, 2087, 2331
Eco81I	CCTNAGG	1 cut(s)	1743
Eco88I	CYCGRG	1 cut(s)	1272
EcoICRI	GAGCTC	1 cut(s)	1008
EcoRI	GAATTC	2 cut(s)	10, 2561
EcoRII	CCWGG	3 cut(s)	1031, 1666, 2427
EcoT14I	CCWWGG	3 cut(s)	115, 563, 1314
EcoT38I	GRGCYC	2 cut(s)	465, 1010
EgeI	GGCGCC	1 cut(s)	136
EheI	GGCGCC	1 cut(s)	136
ErhI	CCWWGG	3 cut(s)	115, 563, 1314
Esp3I	CGTCTC	1 cut(s)	130
FaeI	CATG	4 cut(s)	349, 1150, 2315, 2585
FalI	AAGNNNNNCTT	4 cut(s)	32, 64, 2108, 2140
FatI	CATG	4 cut(s)	345, 1146, 2311, 2581
FbaI	TGATCA	2 cut(s)	631, 1378
FblI	GTMKAC	2 cut(s)	303, 794
Fnu4HI	GCNGC	4 cut(s)	121, 504, 1016, 2120
FokI	GGATG	6 cut(s)	884, 1309, 1539, 1643, 1965, 2046
FriOI	GRGCYC	2 cut(s)	465, 1010
Fsp4HI	GCNGC	4 cut(s)	121, 504, 1016, 2120
FspBI	CTAG	9 cut(s)	116, 324, 885, 1062, 1551, 1685, 1823, 2141, 2608
GlaI	GCGC	2 cut(s)	136, 2310
GluI	GCNGC	4 cut(s)	121, 504, 1016, 2120
GsaI	CCCAGC	1 cut(s)	1296
GsuI	CTGGAG	3 cut(s)	1054, 2370, 2472
HaeII	RGCGCY	1 cut(s)	138
HaeIII	GGCC	3 cut(s)	1204, 1968, 2352
HapII	CCGG	1 cut(s)	1542
HgaI	GACGC	2 cut(s)	112, 1669
HhaI	GCGC	2 cut(s)	137, 2311
Hin1I	GRCGYC	2 cut(s)	123, 135
Hin1II	CATG	4 cut(s)	349, 1150, 2315, 2585
Hin6I	GCGC	2 cut(s)	135, 2309
HinP1I	GCGC	2 cut(s)	135, 2309
HindIII	AAGCTT	3 cut(s)	46, 246, 2183
HinfI	GANTC	9 cut(s)	229, 494, 740, 1091, 1126, 1738, 1795, 1881, 2155
HpaII	CCGG	1 cut(s)	1542
HphI	GGTGA	5 cut(s)	361, 794, 812, 1843, 2423
Hpy166II	GTNNAC	5 cut(s)	304, 795, 1328, 1724, 2392
Hpy8I	GTNNAC	5 cut(s)	304, 795, 1328, 1724, 2392
HpyAV	CCTTC	5 cut(s)	175, 330, 586, 1228, 1460
HpyCH4IV	ACGT	2 cut(s)	1680, 1718
HpyF10VI	GCNNNNNNNGC	6 cut(s)	581, 921, 930, 1547, 1574, 2358
HpyF3I	CTNAG	4 cut(s)	335, 443, 934, 1743
HpySE526I	ACGT	2 cut(s)	1680, 1718
Hsp92I	GRCGYC	2 cut(s)	123, 135
Hsp92II	CATG	4 cut(s)	349, 1150, 2315, 2585
HspAI	GCGC	2 cut(s)	135, 2309
KasI	GGCGCC	1 cut(s)	134
KroI	GCCGGC	1 cut(s)	1541
KroNI	GCCGGC	1 cut(s)	1543
Ksp22I	TGATCA	2 cut(s)	631, 1378
Kzo9I	GATC	5 cut(s)	173, 631, 707, 976, 1378
LmnI	GCTCC	6 cut(s)	164, 468, 589, 1111, 2014, 2247
Lsp1109I	GCAGC	3 cut(s)	490, 1002, 2131
LweI	GCATC	8 cut(s)	601, 835, 873, 1300, 1517, 1943, 1996, 2188
MabI	ACCWGGT	1 cut(s)	2427
MaeI	CTAG	9 cut(s)	116, 324, 885, 1062, 1551, 1685, 1823, 2141, 2608
MaeII	ACGT	2 cut(s)	1680, 1718
MaeIII	GTNAC	5 cut(s)	394, 554, 917, 1453, 2065
MalI	GATC	5 cut(s)	175, 633, 709, 978, 1380
MboI	GATC	5 cut(s)	173, 631, 707, 976, 1378
MflI	RGATCY	3 cut(s)	173, 707, 976
MhlI	GDGCHC	3 cut(s)	465, 1010, 1241
Mly113I	GGCGCC	1 cut(s)	135
MlyI	GAGTC	1 cut(s)	1120
MmeI	TCCRAC	2 cut(s)	81, 1059
MroNI	GCCGGC	1 cut(s)	1541
MroXI	GAANNNNTTC	3 cut(s)	961, 2100, 2481
MseI	TTAA	7 cut(s)	819, 879, 1002, 1194, 1983, 1989, 2507
MslI	CAYNNNNRTG	1 cut(s)	2449
MspCI	CTTAAG	2 cut(s)	1193, 1982
MspI	CCGG	1 cut(s)	1542
MspR9I	CCNGG	3 cut(s)	1033, 1668, 2429
MvaI	CCWGG	3 cut(s)	1033, 1668, 2429
MwoI	GCNNNNNNNGC	6 cut(s)	581, 921, 930, 1547, 1574, 2358
NaeI	GCCGGC	1 cut(s)	1543
NarI	GGCGCC	1 cut(s)	135
NdeII	GATC	5 cut(s)	173, 631, 707, 976, 1378
NgoMIV	GCCGGC	1 cut(s)	1541
NlaIII	CATG	4 cut(s)	349, 1150, 2315, 2585
NmuCI	GTSAC	2 cut(s)	1453, 2065
NspI	RCATGY	1 cut(s)	2315
PaeI	GCATGC	1 cut(s)	2315
PaeR7I	CTCGAG	1 cut(s)	1272
PagI	TCATGA	1 cut(s)	2581
PceI	AGGCCT	1 cut(s)	1204
PcsI	WCGNNNNNNNCGW	1 cut(s)	20
PdiI	GCCGGC	1 cut(s)	1543
PdmI	GAANNNNTTC	3 cut(s)	961, 2100, 2481
PfeI	GAWTC	8 cut(s)	229, 494, 740, 1091, 1738, 1795, 1881, 2155
PfoI	TCCNGGA	1 cut(s)	1666
PkrI	GCNGC	4 cut(s)	122, 505, 1017, 2121
PleI	GAGTC	1 cut(s)	1120
PluTI	GGCGCC	1 cut(s)	138
PpsI	GAGTC	1 cut(s)	1120
Psp124BI	GAGCTC	1 cut(s)	1010
Psp6I	CCWGG	3 cut(s)	1031, 1666, 2427
PspFI	CCCAGC	1 cut(s)	1292
PspGI	CCWGG	3 cut(s)	1031, 1666, 2427
PspPI	GGNCC	2 cut(s)	264, 1966
PstI	CTGCAG	3 cut(s)	228, 413, 1412
PstNI	CAGNNNCTG	3 cut(s)	334, 503, 921
PsuI	RGATCY	3 cut(s)	173, 707, 976
RsaI	GTAC	5 cut(s)	344, 889, 1419, 1972, 2263
RsaNI	GTAC	5 cut(s)	343, 888, 1418, 1971, 2262
RseI	CAYNNNNRTG	1 cut(s)	2449
SacI	GAGCTC	1 cut(s)	1010
SaqAI	TTAA	7 cut(s)	819, 879, 1002, 1194, 1983, 1989, 2507
SatI	GCNGC	4 cut(s)	121, 504, 1016, 2120
Sau3AI	GATC	5 cut(s)	173, 631, 707, 976, 1378
Sau96I	GGNCC	2 cut(s)	264, 1966
SchI	GAGTC	1 cut(s)	1120
ScrFI	CCNGG	3 cut(s)	1033, 1668, 2429
SduI	GDGCHC	3 cut(s)	465, 1010, 1241
SexAI	ACCWGGT	1 cut(s)	2427
SfaNI	GCATC	8 cut(s)	601, 835, 873, 1300, 1517, 1943, 1996, 2188
SfcI	CTRYAG	5 cut(s)	224, 409, 1206, 1408, 2289
SfoI	GGCGCC	1 cut(s)	136
Sfr274I	CTCGAG	1 cut(s)	1272
SinI	GGWCC	1 cut(s)	264
SlaI	CTCGAG	1 cut(s)	1272
SmiMI	CAYNNNNRTG	1 cut(s)	2449
SmlI	CTYRAG	6 cut(s)	517, 1009, 1193, 1272, 1982, 2186
SmoI	CTYRAG	6 cut(s)	517, 1009, 1193, 1272, 1982, 2186
SphI	GCATGC	1 cut(s)	2315
SseBI	AGGCCT	1 cut(s)	1204
SsiI	CCGC	3 cut(s)	120, 735, 2361
SspDI	GGCGCC	1 cut(s)	134
SspI	AATATT	1 cut(s)	809
SspMI	CTAG	9 cut(s)	116, 324, 885, 1062, 1551, 1685, 1823, 2141, 2608
SstI	GAGCTC	1 cut(s)	1010
StuI	AGGCCT	1 cut(s)	1204
StyD4I	CCNGG	3 cut(s)	1031, 1666, 2427
StyI	CCWWGG	3 cut(s)	115, 563, 1314
TaiI	ACGT	2 cut(s)	1683, 1721
TaqI	TCGA	6 cut(s)	251, 293, 955, 1273, 2158, 2341
TatI	WGTACW	2 cut(s)	1417, 2261
TauI	GCSGC	1 cut(s)	123
TfiI	GAWTC	8 cut(s)	229, 494, 740, 1091, 1738, 1795, 1881, 2155
Tru1I	TTAA	7 cut(s)	819, 879, 1002, 1194, 1983, 1989, 2507
Tru9I	TTAA	7 cut(s)	819, 879, 1002, 1194, 1983, 1989, 2507
TscAI	CASTG	9 cut(s)	321, 460, 718, 1246, 1412, 1537, 1965, 2394, 2572
TseFI	GTSAC	2 cut(s)	1453, 2065
TseI	GCWGC	3 cut(s)	503, 1015, 2119
Tsp45I	GTSAC	2 cut(s)	1453, 2065
TspDTI	ATGAA	6 cut(s)	774, 888, 1242, 1452, 1659, 2598
TspGWI	ACGGA	1 cut(s)	1156
TspRI	CASTG	9 cut(s)	321, 460, 718, 1246, 1412, 1537, 1965, 2394, 2572
Vha464I	CTTAAG	2 cut(s)	1193, 1982
VpaK11BI	GGWCC	1 cut(s)	264
XapI	RAATTY	5 cut(s)	10, 940, 1248, 2365, 2561
XbaI	TCTAGA	2 cut(s)	1684, 2140
XceI	RCATGY	1 cut(s)	2315
XhoI	CTCGAG	1 cut(s)	1272
XmaJI	CCTAGG	1 cut(s)	115
XmiI	GTMKAC	2 cut(s)	303, 794
XmnI	GAANNNNTTC	3 cut(s)	961, 2100, 2481
XspI	CTAG	9 cut(s)	116, 324, 885, 1062, 1551, 1685, 1823, 2141, 2608

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.