Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0004782

Physical Location & Seq

Genomic Coordinates Forward (+)

9936560 .. 9949011

Gene Structure 12452 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G006270.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3435 bp

ATGAGTTTGAATTCGTCAAAATCGTCTGGTTCAAAATCCAAGCGAAGCTTCAATTCCAACAATTCCACTAACAACAACAACAAGAAGACTAAGATTAGCACCAATCAGAAAACCCTAGGCGGCGTCTCTTGGGGGCCAATTCTCTCTCCTCCTCTCGCTCCTCCTTCCAAAGATCCCCCTTTCCCGATTTCGGCAGGTTTTGTTCTTTTACTGATTGGAATTAGGTTATGTTTGTTGTGTTTGTTGGGCAGTTATATGGTAGAGAAGAACAGGAAACTGCAGAATCAGTTTGATAGTGAAGCTTCGAGTTGTTTGGGGTCCGAGAGGAGTATTTTTCGTGGGGTTTCGATTTTCGTAGATGGTTTCACAGTGCCTTCTAGTCAGGAACTGAGAGCGTACATGGTGAATTATGGTGGTAGATATGAGAATTACTTTTCACGGCGTCGTGTTACACATATCATCTGCAGTAATCTTCCTGACAGTAAAATAAAAAATCTGAGGTCTTTCAGTGGAGGGCTCCCAGTTGTGAAGCCTAACTGGATTGTAGATTCAGTTGCTGCTAACAAACTCTTGAGTTGGGTTCCTTACCAACTTGAGCAGGTTTCTTGTAACCAACCAAGGTTGTCAGCCTTCTTTGCTCCGAAGATAATCCCCAACTGTGATGATGCTTTGAGAGATACATCTGATCAAGTGAAACCTGAAAGTGAGGATACATCTTTGGTAGGAACCAGATTAGAAGATGATAACAAGTCAGTATGCAGATCCACTGAACACGAACAGGAAAGTAGCGGAGAATCTGATGATATGATATATGAAAACACCGATGGACAATTTGGTGAGGAGTTGTATACAGGTGAAAAATATTCTGAAGTTAAACAAGAAGAAACAAGCGGAGAATCTGATGATATGATATATGAAAACACCGATGGACAATTTGGTGAGGAGTTGTATACAGGTGAAAAATATTCTGAATACCCACAGCACTCTGCTTCAGTTAGCAGTAACTGCTTGCCAAGCTCAGAAAATTTTGGGTCTAATCGAAGTCATTCAACTCTTGGGGATCCCAATTTTGTGGAAAATTATTTTAAGAGCTCAAGGCTGCACTTTATAGGAACCTGGAGAAATCGGTATCGCAAGCATTTTCCTAGTTCGTCTAAAGGGTTGGACAACACAGATTCTAACCGTTGTGCTCCTGATAGTTCTCTGAAGACTCCCATTATACATATTGACATGGACTGTTTTTTTGTGTCCGTGGTCATCAGGCAACGCCCTGAACTTAAGGATAGGCCTGTAGCTGTATGTCATTCTGATAATCCAAAGGGCACTGCTGAAATTTCATCTGCAAACTACCCTGCTCGAGATTATGGAGTGAGGGCTGGGATGTTTGTCAGAGATGCTAAGGCACTTTGTCCACACCTTGTCATTCTTCCTTATGATTTTGAAGCGTATGAGGAGGTAGCTGATCAGTTCTATGACATTCTGCATAAGCACTGCAGAAAAGTACAAGCAGTAAGCTGTGATGAAGCATTTTTAGATGTCACATACTTGGAAGGGGTAGATATGGATATGTTAGCCTCAACTGTAAGACAAGAGATTTTTGAGACTACTGGATGCACTGCAAGTGCTGGCATAGCTAGAAATATGCTGATGGCTCGCCTTGCAACAAGAACTGCTAAACCAGATGGTCAATGCAACATTCCTCCCGAAAGGGTGGATGATTATTTATATGAACTTCCCATCAAGACGCTTCCAGGAATAGGACACGTTCTAGAGGAGAAGTTGAAGAAGCGAAATGTTTTGACTTGTGGACAGTTGCGTATGATTCCTAAGGACTCTCTTCAAAAGGACTTCGGAATAAAAACTGGGGAGATGTTGTGGAATCATAGTAGAGGCATAGATAATCGGCTAGTTGGGGTGATTCAGGAAAGTAAGTCTATTGGTGCTGAAGTGAATTGGGGTGTGAGATTCAAGGATTTGAAAGATAGTCATCACTTCCTTTCAAATCTTTGCAAGGAGGTTTCTTTACGCTTGCAAGGATGCACAGTGCAGGGTCGTACTTTTACCCTTAAGATTAAGAAAAGAAGAAAAGATGCACAGGAGCCTGTGAAGTATATGGGATGTGGAGACTGTGAAAACTTGAGCCACTCTGTCACGGTTCCAGTTGCTACTGATGATGTGGAAGTGCTTCAGAGAATAACAAAGCAGCTCTTTGGGTTTTTCTCTCTAGATGTCAAGGAGATTCGAGGTATTGGTTTGCAAGTTTCCAAGCTTGAGAGTGTAGATGCTTCAAAGCAAGGACCTGGAAAAAACTCTTTGAAATCGTGGCTCCAATCTGCCAAAGCAAGTACAGAAGAACAATCCAATACTTATTCTGTAGATGATGGGAAGAGGGCGCATGCAGATTGTGAGGGTCGGGGAACCACTTCCGGCCAGCTATGCGGAAATTCACTTGGTATTCCAACTCCAGTGGACAATCATCGGTGTAGTGGTGAAACTTCTGTGAACCAGGTTTCAGCACCACCACCTTTATGTCATCTTGATTTAGGAGTTATTGAAAGTCTTCCTCCAGAACTTTTTACGGAATTAAACGGGATCTATGGTGGCAAGTTGGTTGATTTTGTTGCTAAAAATAAACGAGAATTCAGTGCAACAACATCTCATGAACGAGTAGATGGTGCAAAGAACGGGAGTGAGAGTCATCTCTTCAATGATATGCACCTGCAGGATGAAAATATATTAGAACCCAAGCATACAGTTGTAGAAAAACAAGCAATGCCTTCTTCTGTGGGAGGGTCCAGTGATGTGGCAGCTTCAACATCAGGTCTTGGAAATACTGATATAATGCCTGCATCTTTAAGTCAAGTAGATCCCTTGGTGTTGCAACAATTACCTCAAGAACTGAGAGTTGACTTACTTGAGCAGCTTCCTGCACACAGGAGATATGACTTAGCTTCAAGTGCTGCCTTGGACCCTCTTGCAGAGATACCTGGAGAATCAATAGGTATGAGGGACGGAAATCATTCTGGATCACATGATCTTGCATTTAACAAGCTTTGGATTGGAAATCCTCCACAGTGGGTTGAAGAGTTCAAAGCCAGCAAATGCATGATATTAAATATTCTTGCTGAGATGTATGACAAATCAGGGTCAAGTGGAAACTTATCTGTAATTCTGCGCAGTACTATCATGGAATGTCAACATCCTCTAGATTCAAGTTCTGACTGTTGGATTCAAGCTGTATACAGCTTCTTTGAGCTTCTCAGGCAGTATGTTACATTAAAGATCGATTCAGATATTGAAGAGATCTATGTTTGTTTTCGGCTTCTTAGAAGGTTTACAACGAAGTCAAAGTTTTTCTTACAAGTATATAGTGATGTCTTTCCTTACCTTCAGGCATCTTTCACTGATAGCTATGGAGGAAACTTGCACATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000002 GO:0000731 GO:0002200 GO:0002206 GO:0002376 GO:0002377 GO:0002440 GO:0002520 GO:0002565 GO:0003674 GO:0003824 GO:0003887 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006281 GO:0006301 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007005 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010224 GO:0016043 GO:0016444 GO:0016445 GO:0016740 GO:0016772 GO:0016779 GO:0017125 GO:0018130 GO:0019438 GO:0019985 GO:0031974 GO:0031981 GO:0032042 GO:0032501 GO:0032502 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0035822 GO:0035861 GO:0042276 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043504 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048731 GO:0048856 GO:0050896 GO:0051716 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0090304 GO:0090734 GO:0140097 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03460 map03460

Pfam Domains

Protein Families

BRCT_2 IMS IMS_C IMS_HHH LIG3_BRCT

Protein Analysis

1144

Amino Acids

127.21

Weight (kDa)

5.77

Isoelectric Point (pI)

48.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BRCT	PF00533	109 - 184	1.9e-09	BRCA1 C Terminus (BRCT) domain
BRCT_2	PF16589	111 - 196	6.9e-07	BRCT domain
LIG3_BRCT	PF16759	111 - 187	1.1e-06	DNA ligase 3 BRCT domain
IMS	PF00817	409 - 556	2e-45	impB/mucB/samB family
IMS_HHH	PF11798	568 - 599	1.3e-06	IMS family HHH motif
IMS_HHH_1	PF21999	582 - 633	1.4e-19	DNA polymerase-iota, thumb domain
IMS_C	PF11799	643 - 762	3.7e-18	impB/mucB/samB family C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004782)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44750 AT5G44750
fragaria_vesca	FvH4_1g23540 FvH4_2g04972
malus_domestica	MD05G1091100.v1.1 MD10G1098400.v1.1
prunus_persica	Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1 Prupe.8G136700_v2.0.a1
pyrus_communis	pycom10g08430
rosa_chinensis	RchiOBHm_Chr6g0254741
rosa_laevigata	RLG00000014893
rosa_multiflora	Rmu_sc0007716.1_g000003
rosa_roxburghii	Rroxscaffold_7G00210560
rosa_rugosa	Rorug05G0554600
rosa_samantha	Rh6AG071600 Rh6BG063800 Rh6CG063200 Rh6DG060300
rosa_wichuraiana	Rw6G006270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2727
Acc16I	TGCGCA	1 cut(s)	3176
Acc36I	ACCTGC	3 cut(s)	185, 589, 2727
AccI	GTMKAC	3 cut(s)	848, 950, 3240
AciI	CCGC	4 cut(s)	120, 789, 891, 2439
AclWI	GGATC	7 cut(s)	167, 756, 1055, 1068, 2600, 2861, 3034
AcoI	YGGCCR	1 cut(s)	2428
AcsI	RAATTY	5 cut(s)	10, 1024, 1332, 2443, 2639
AcuI	CTGAAG	6 cut(s)	888, 975, 1226, 1965, 2171, 3374
AcyI	GRCGYC	2 cut(s)	123, 442
AfaI	GTAC	5 cut(s)	398, 1503, 2056, 2347, 3181
AfiI	CCNNNNNNNGG	2 cut(s)	190, 1757
AflII	CTTAAG	2 cut(s)	1277, 2066
AflIII	ACRYGT	1 cut(s)	1762
AjnI	CCWGG	5 cut(s)	1115, 1750, 2299, 2505, 2986
AjuI	GAANNNNNNNTTGG	2 cut(s)	32, 64
Alw21I	GWGCWC	2 cut(s)	1094, 1192
Alw26I	GTCTC	3 cut(s)	130, 1595, 2118
AlwI	GGATC	7 cut(s)	167, 756, 1055, 1068, 2600, 2861, 3034
AlwNI	CAGNNNCTG	3 cut(s)	388, 557, 1005
Ama87I	CYCGRG	1 cut(s)	1356
AoxI	GGCC	3 cut(s)	134, 1286, 2428
ApeKI	GCWGC	6 cut(s)	557, 1099, 2203, 2807, 2920, 2960
ApoI	RAATTY	5 cut(s)	10, 1024, 1332, 2443, 2639
ArsI	GACNNNNNNTTYG	2 cut(s)	1226, 1258
Asp700I	GAANNNNTTC	5 cut(s)	1045, 2184, 2422, 2559, 3019
AspA2I	CCTAGG	1 cut(s)	115
AspLEI	GCGC	2 cut(s)	2395, 3177
AspS9I	GGNCC	5 cut(s)	134, 318, 2297, 2793, 2968
AsuHPI	GGTGA	7 cut(s)	415, 848, 866, 950, 968, 1927, 2501
AvaI	CYCGRG	1 cut(s)	1356
AvaII	GGWCC	4 cut(s)	318, 2297, 2793, 2968
AvrII	CCTAGG	1 cut(s)	115
AxyI	CCTNAGG	1 cut(s)	1827
BaeGI	GKGCMC	1 cut(s)	1325
BamHI	GGATCC	1 cut(s)	1060
BanII	GRGCYC	2 cut(s)	519, 1094
BbsI	GAAGAC	3 cut(s)	92, 1214, 2552
Bbv12I	GWGCWC	2 cut(s)	1094, 1192
BbvI	GCAGC	6 cut(s)	544, 1086, 2215, 2819, 2932, 2947
BccI	CCATC	8 cut(s)	353, 818, 920, 1642, 1676, 1745, 2375, 2666
BceAI	ACGGC	1 cut(s)	455
BciT130I	CCWGG	5 cut(s)	1117, 1752, 2301, 2507, 2988
BciVI	GTATCC	1 cut(s)	703
BclI	TGATCA	2 cut(s)	685, 1462
BcoDI	GTCTC	3 cut(s)	130, 1595, 2118
BfaI	CTAG	8 cut(s)	116, 378, 1146, 1635, 1769, 1907, 2225, 3206
BfmI	CTRYAG	6 cut(s)	278, 463, 1290, 1492, 2373, 2720
BfrI	CTTAAG	2 cut(s)	1277, 2066
BfuAI	ACCTGC	3 cut(s)	185, 589, 2727
BfuI	GTATCC	1 cut(s)	703
BglII	AGATCT	1 cut(s)	3303
BisI	GCNGC	7 cut(s)	121, 558, 1100, 2204, 2808, 2921, 2961
BlnI	CCTAGG	1 cut(s)	115
BlsI	GCNGC	7 cut(s)	122, 559, 1101, 2205, 2809, 2922, 2962
BmcAI	AGTACT	1 cut(s)	3181
Bme1390I	CCNGG	5 cut(s)	1117, 1752, 2301, 2507, 2988
Bme18I	GGWCC	4 cut(s)	318, 2297, 2793, 2968
BmeT110I	CYCGRG	1 cut(s)	1356
BmgT120I	GGNCC	5 cut(s)	134, 318, 2297, 2793, 2968
BmrFI	CCNGG	5 cut(s)	1117, 1752, 2301, 2507, 2988
BmrI	ACTGGG	2 cut(s)	515, 1872
BmsI	GCATC	8 cut(s)	655, 1384, 1601, 2027, 2080, 2272, 2858, 3404
BmuI	ACTGGG	2 cut(s)	515, 1872
BpiI	GAAGAC	3 cut(s)	92, 1214, 2552
BpmI	CTGGAG	4 cut(s)	1138, 2448, 2550, 3009
Bpu10I	CCTNAGC	1 cut(s)	1398
BpuEI	CTTGAG	7 cut(s)	592, 614, 1078, 2158, 2291, 2877, 2936
Bsa29I	ATCGAT	1 cut(s)	3285
BsaBI	GATNNNNATC	1 cut(s)	1986
BsaHI	GRCGYC	2 cut(s)	123, 442
BsaJI	CCNNGG	5 cut(s)	115, 617, 1251, 2871, 2964
BsaXI	ACNNNNNCTCC	4 cut(s)	1859, 1889, 2680, 2710
Bsc4I	CCNNNNNNNGG	2 cut(s)	190, 1757
Bse1I	ACTGG	7 cut(s)	521, 542, 1612, 1867, 2159, 2465, 2796
Bse21I	CCTNAGG	1 cut(s)	1827
Bse3DI	GCAATG	1 cut(s)	2778
Bse8I	GATNNNNATC	1 cut(s)	1986
BseBI	CCWGG	5 cut(s)	1117, 1752, 2301, 2507, 2988
BseCI	ATCGAT	1 cut(s)	3285
BseDI	CCNNGG	5 cut(s)	115, 617, 1251, 2871, 2964
BseGI	GGATG	7 cut(s)	1386, 1616, 1720, 2042, 2123, 2731, 3199
BseJI	GATNNNNATC	1 cut(s)	1986
BseLI	CCNNNNNNNGG	2 cut(s)	190, 1757
BseMI	GCAATG	1 cut(s)	2778
BseMII	CTCAG	6 cut(s)	380, 488, 1032, 2891, 3117, 3274
BseNI	ACTGG	7 cut(s)	521, 542, 1612, 1867, 2159, 2465, 2796
BseRI	GAGGAG	8 cut(s)	138, 141, 150, 340, 854, 956, 1466, 1787
BseSI	GKGCMC	1 cut(s)	1325
BseXI	GCAGC	6 cut(s)	544, 1086, 2215, 2819, 2932, 2947
BseYI	CCCAGC	1 cut(s)	1376
BsgI	GTGCAG	3 cut(s)	1085, 2066, 2913
BshFI	GGCC	3 cut(s)	136, 1288, 2430
BshVI	ATCGAT	1 cut(s)	3285
BsiHKAI	GWGCWC	2 cut(s)	1094, 1192
BsiHKCI	CYCGRG	1 cut(s)	1356
BsiSI	CCGG	1 cut(s)	2427
BslFI	GGGAC	1 cut(s)	3023
BslI	CCNNNNNNNGG	2 cut(s)	190, 1757
BsmAI	GTCTC	3 cut(s)	130, 1595, 2118
BsmBI	CGTCTC	1 cut(s)	130
BsmFI	GGGAC	1 cut(s)	3023
BsnI	GGCC	3 cut(s)	136, 1288, 2430
BsoBI	CYCGRG	1 cut(s)	1356
Bsp1286I	GDGCHC	4 cut(s)	519, 1094, 1192, 1325
BspACI	CCGC	4 cut(s)	120, 789, 891, 2439
BspANI	GGCC	3 cut(s)	136, 1288, 2430
BspCNI	CTCAG	6 cut(s)	381, 489, 1031, 2892, 3118, 3273
BspDI	ATCGAT	1 cut(s)	3285
BspHI	TCATGA	1 cut(s)	2659
BspMAI	CTGCAG	4 cut(s)	282, 467, 1496, 2724
BspMI	ACCTGC	3 cut(s)	185, 589, 2727
BspPI	GGATC	7 cut(s)	167, 756, 1055, 1068, 2600, 2861, 3034
BspTI	CTTAAG	2 cut(s)	1277, 2066
BsrDI	GCAATG	1 cut(s)	2778
BsrI	ACTGG	7 cut(s)	521, 542, 1612, 1867, 2159, 2465, 2796
BssECI	CCNNGG	5 cut(s)	115, 617, 1251, 2871, 2964
BssNAI	GTATAC	3 cut(s)	849, 951, 3241
BssNI	GRCGYC	2 cut(s)	123, 442
BssT1I	CCWWGG	4 cut(s)	115, 617, 2871, 2964
Bst1107I	GTATAC	3 cut(s)	849, 951, 3241
Bst2UI	CCWGG	5 cut(s)	1117, 1752, 2301, 2507, 2988
Bst6I	CTCTTC	5 cut(s)	1842, 2381, 2708, 3078, 3294
BstACI	GRCGYC	2 cut(s)	123, 442
BstAFI	CTTAAG	2 cut(s)	1277, 2066
BstAPI	GCANNNNNTGC	1 cut(s)	1005
BstC8I	GCNNGC	9 cut(s)	1010, 1136, 1627, 1654, 2030, 2397, 2432, 2847, 3097
BstDSI	CCRYGG	1 cut(s)	1251
BstF5I	GGATG	7 cut(s)	1386, 1616, 1720, 2042, 2123, 2731, 3199
BstHHI	GCGC	2 cut(s)	2395, 3177
BstMAI	GTCTC	3 cut(s)	130, 1595, 2118
BstMWI	GCNNNNNNNGC	8 cut(s)	635, 1005, 1014, 1631, 1658, 2436, 2957, 3262
BstNI	CCWGG	5 cut(s)	1117, 1752, 2301, 2507, 2988
BstNSI	RCATGY	1 cut(s)	2399
BstSCI	CCNGG	5 cut(s)	1115, 1750, 2299, 2505, 2986
BstSFI	CTRYAG	6 cut(s)	278, 463, 1290, 1492, 2373, 2720
BstSLI	GKGCMC	1 cut(s)	1325
BstV1I	GCAGC	6 cut(s)	544, 1086, 2215, 2819, 2932, 2947
BstV2I	GAAGAC	3 cut(s)	92, 1214, 2552
BstX2I	RGATCY	6 cut(s)	172, 761, 1060, 2592, 2866, 3303
BstXI	CCANNNNNNTGG	2 cut(s)	1072, 2803
BstYI	RGATCY	6 cut(s)	172, 761, 1060, 2592, 2866, 3303
BstZ17I	GTATAC	3 cut(s)	849, 951, 3241
Bsu15I	ATCGAT	1 cut(s)	3285
Bsu36I	CCTNAGG	1 cut(s)	1827
BsuI	GTATCC	1 cut(s)	703
BsuRI	GGCC	3 cut(s)	136, 1288, 2430
BsuTUI	ATCGAT	1 cut(s)	3285
BtgI	CCRYGG	1 cut(s)	1251
BtsCI	GGATG	7 cut(s)	1386, 1616, 1720, 2042, 2123, 2731, 3199
BtsI	GCAGTG	3 cut(s)	1323, 1489, 1614
BveI	ACCTGC	3 cut(s)	185, 589, 2727
Cac8I	GCNNGC	9 cut(s)	1010, 1136, 1627, 1654, 2030, 2397, 2432, 2847, 3097
CaiI	CAGNNNCTG	3 cut(s)	388, 557, 1005
CciI	TCATGA	1 cut(s)	2659
CfoI	GCGC	2 cut(s)	2395, 3177
Cfr13I	GGNCC	5 cut(s)	134, 318, 2297, 2793, 2968
ClaI	ATCGAT	1 cut(s)	3285
CseI	GACGC	3 cut(s)	112, 431, 1753
CsiI	ACCWGGT	1 cut(s)	2505
Csp6I	GTAC	5 cut(s)	397, 1502, 2055, 2346, 3180
CspCI	CAANNNNNGTGG	2 cut(s)	2448, 2483
CviAII	CATG	7 cut(s)	400, 1231, 2396, 2660, 3032, 3106, 3187
CviQI	GTAC	5 cut(s)	397, 1502, 2055, 2346, 3180
EaeI	YGGCCR	1 cut(s)	2428
Eam1104I	CTCTTC	5 cut(s)	1842, 2381, 2708, 3078, 3294
EarI	CTCTTC	5 cut(s)	1842, 2381, 2708, 3078, 3294
Ecl136II	GAGCTC	1 cut(s)	1092
Eco130I	CCWWGG	4 cut(s)	115, 617, 2871, 2964
Eco147I	AGGCCT	1 cut(s)	1288
Eco24I	GRGCYC	2 cut(s)	519, 1094
Eco47I	GGWCC	4 cut(s)	318, 2297, 2793, 2968
Eco53kI	GAGCTC	1 cut(s)	1092
Eco57I	CTGAAG	6 cut(s)	888, 975, 1226, 1965, 2171, 3374
Eco81I	CCTNAGG	1 cut(s)	1827
Eco88I	CYCGRG	1 cut(s)	1356
EcoICRI	GAGCTC	1 cut(s)	1092
EcoO109I	RGGNCCY	1 cut(s)	2297
EcoRI	GAATTC	2 cut(s)	10, 2639
EcoRII	CCWGG	5 cut(s)	1115, 1750, 2299, 2505, 2986
EcoT14I	CCWWGG	4 cut(s)	115, 617, 2871, 2964
EcoT22I	ATGCAT	1 cut(s)	3107
EcoT38I	GRGCYC	2 cut(s)	519, 1094
ErhI	CCWWGG	4 cut(s)	115, 617, 2871, 2964
Esp3I	CGTCTC	1 cut(s)	130
FaeI	CATG	7 cut(s)	403, 1234, 2399, 2663, 3035, 3109, 3190
FalI	AAGNNNNNCTT	6 cut(s)	32, 64, 2192, 2224, 3341, 3373
FaqI	GGGAC	1 cut(s)	3023
FatI	CATG	7 cut(s)	399, 1230, 2395, 2659, 3031, 3105, 3186
FbaI	TGATCA	2 cut(s)	685, 1462
FblI	GTMKAC	3 cut(s)	848, 950, 3240
Fnu4HI	GCNGC	7 cut(s)	121, 558, 1100, 2204, 2808, 2921, 2961
FokI	GGATG	7 cut(s)	1393, 1623, 1727, 2049, 2130, 2738, 3186
FriOI	GRGCYC	2 cut(s)	519, 1094
Fsp4HI	GCNGC	7 cut(s)	121, 558, 1100, 2204, 2808, 2921, 2961
FspBI	CTAG	8 cut(s)	116, 378, 1146, 1635, 1769, 1907, 2225, 3206
FspI	TGCGCA	1 cut(s)	3176
GlaI	GCGC	2 cut(s)	2394, 3176
GluI	GCNGC	7 cut(s)	121, 558, 1100, 2204, 2808, 2921, 2961
GsaI	CCCAGC	1 cut(s)	1380
GsuI	CTGGAG	4 cut(s)	1138, 2448, 2550, 3009
HaeIII	GGCC	3 cut(s)	136, 1288, 2430
HapII	CCGG	1 cut(s)	2427
HgaI	GACGC	3 cut(s)	112, 431, 1753
HhaI	GCGC	2 cut(s)	2395, 3177
Hin1I	GRCGYC	2 cut(s)	123, 442
Hin1II	CATG	7 cut(s)	403, 1234, 2399, 2663, 3035, 3109, 3190
Hin6I	GCGC	2 cut(s)	2393, 3175
HinP1I	GCGC	2 cut(s)	2393, 3175
HincII	GTYRAC	2 cut(s)	2908, 3197
HindII	GTYRAC	2 cut(s)	2908, 3197
HindIII	AAGCTT	4 cut(s)	46, 300, 2267, 3050
HpaII	CCGG	1 cut(s)	2427
HphI	GGTGA	7 cut(s)	415, 848, 866, 950, 968, 1927, 2501
Hpy99I	CGWCG	1 cut(s)	447
HpyAV	CCTTC	7 cut(s)	174, 384, 640, 1544, 2787, 3324, 3398
HpyCH4IV	ACGT	1 cut(s)	1764
HpyF10VI	GCNNNNNNNGC	8 cut(s)	635, 1005, 1014, 1631, 1658, 2436, 2957, 3262
HpySE526I	ACGT	1 cut(s)	1764
Hsp92I	GRCGYC	2 cut(s)	123, 442
Hsp92II	CATG	7 cut(s)	403, 1234, 2399, 2663, 3035, 3109, 3190
HspAI	GCGC	2 cut(s)	2393, 3175
Ksp22I	TGATCA	2 cut(s)	685, 1462
LmnI	GCTCC	6 cut(s)	163, 522, 643, 1195, 2098, 2331
Lsp1109I	GCAGC	6 cut(s)	544, 1086, 2215, 2819, 2932, 2947
LweI	GCATC	8 cut(s)	655, 1384, 1601, 2027, 2080, 2272, 2858, 3404
MabI	ACCWGGT	1 cut(s)	2505
MaeI	CTAG	8 cut(s)	116, 378, 1146, 1635, 1769, 1907, 2225, 3206
MaeII	ACGT	1 cut(s)	1764
MaeIII	GTNAC	6 cut(s)	448, 608, 1001, 1537, 2149, 3271
MflI	RGATCY	6 cut(s)	172, 761, 1060, 2592, 2866, 3303
MhlI	GDGCHC	4 cut(s)	519, 1094, 1192, 1325
MlyI	GAGTC	3 cut(s)	1204, 1826, 2704
MmeI	TCCRAC	4 cut(s)	81, 1143, 2483, 3206
Mph1103I	ATGCAT	1 cut(s)	3107
MroXI	GAANNNNTTC	5 cut(s)	1045, 2184, 2422, 2559, 3019
MslI	CAYNNNNRTG	1 cut(s)	2527
MspCI	CTTAAG	2 cut(s)	1277, 2066
MspI	CCGG	1 cut(s)	2427
MspR9I	CCNGG	5 cut(s)	1117, 1752, 2301, 2507, 2988
MvaI	CCWGG	5 cut(s)	1117, 1752, 2301, 2507, 2988
MwoI	GCNNNNNNNGC	8 cut(s)	635, 1005, 1014, 1631, 1658, 2436, 2957, 3262
NlaIII	CATG	7 cut(s)	403, 1234, 2399, 2663, 3035, 3109, 3190
NmuCI	GTSAC	2 cut(s)	1537, 2149
NsbI	TGCGCA	1 cut(s)	3176
NsiI	ATGCAT	1 cut(s)	3107
NspI	RCATGY	1 cut(s)	2399
PaeI	GCATGC	1 cut(s)	2399
PaeR7I	CTCGAG	1 cut(s)	1356
PagI	TCATGA	1 cut(s)	2659
PaqCI	CACCTGC	1 cut(s)	2727
PceI	AGGCCT	1 cut(s)	1288
PcsI	WCGNNNNNNNCGW	1 cut(s)	20
PdmI	GAANNNNTTC	5 cut(s)	1045, 2184, 2422, 2559, 3019
PfoI	TCCNGGA	1 cut(s)	1750
PkrI	GCNGC	7 cut(s)	122, 559, 1101, 2205, 2809, 2922, 2962
PleI	GAGTC	3 cut(s)	1204, 1826, 2703
PpsI	GAGTC	3 cut(s)	1204, 1826, 2703
PpuMI	RGGWCCY	1 cut(s)	2297
Psp124BI	GAGCTC	1 cut(s)	1094
Psp5II	RGGWCCY	1 cut(s)	2297
Psp6I	CCWGG	5 cut(s)	1115, 1750, 2299, 2505, 2986
PspFI	CCCAGC	1 cut(s)	1376
PspGI	CCWGG	5 cut(s)	1115, 1750, 2299, 2505, 2986
PspPI	GGNCC	5 cut(s)	134, 318, 2297, 2793, 2968
PspPPI	RGGWCCY	1 cut(s)	2297
PstI	CTGCAG	4 cut(s)	282, 467, 1496, 2724
PstNI	CAGNNNCTG	3 cut(s)	388, 557, 1005
PsuI	RGATCY	6 cut(s)	172, 761, 1060, 2592, 2866, 3303
RsaI	GTAC	5 cut(s)	398, 1503, 2056, 2347, 3181
RsaNI	GTAC	5 cut(s)	397, 1502, 2055, 2346, 3180
RseI	CAYNNNNRTG	1 cut(s)	2527
SacI	GAGCTC	1 cut(s)	1094
SatI	GCNGC	7 cut(s)	121, 558, 1100, 2204, 2808, 2921, 2961
Sau96I	GGNCC	5 cut(s)	134, 318, 2297, 2793, 2968
SbfI	CCTGCAGG	1 cut(s)	2724
ScaI	AGTACT	1 cut(s)	3181
SchI	GAGTC	3 cut(s)	1204, 1826, 2704
ScrFI	CCNGG	5 cut(s)	1117, 1752, 2301, 2507, 2988
SdaI	CCTGCAGG	1 cut(s)	2724
SduI	GDGCHC	4 cut(s)	519, 1094, 1192, 1325
SexAI	ACCWGGT	1 cut(s)	2505
SfaNI	GCATC	8 cut(s)	655, 1384, 1601, 2027, 2080, 2272, 2858, 3404
SfcI	CTRYAG	6 cut(s)	278, 463, 1290, 1492, 2373, 2720
Sfr274I	CTCGAG	1 cut(s)	1356
SinI	GGWCC	4 cut(s)	318, 2297, 2793, 2968
SlaI	CTCGAG	1 cut(s)	1356
SmiMI	CAYNNNNRTG	1 cut(s)	2527
SphI	GCATGC	1 cut(s)	2399
Sse8387I	CCTGCAGG	1 cut(s)	2724
SseBI	AGGCCT	1 cut(s)	1288
SsiI	CCGC	4 cut(s)	120, 789, 891, 2439
SspI	AATATT	3 cut(s)	863, 965, 3118
SspMI	CTAG	8 cut(s)	116, 378, 1146, 1635, 1769, 1907, 2225, 3206
SstI	GAGCTC	1 cut(s)	1094
StuI	AGGCCT	1 cut(s)	1288
StyD4I	CCNGG	5 cut(s)	1115, 1750, 2299, 2505, 2986
StyI	CCWWGG	4 cut(s)	115, 617, 2871, 2964
TaiI	ACGT	1 cut(s)	1767
TaqI	TCGA	6 cut(s)	305, 347, 1039, 1357, 2242, 3285
TatI	WGTACW	3 cut(s)	1501, 2345, 3179
TauI	GCSGC	1 cut(s)	123
TseFI	GTSAC	2 cut(s)	1537, 2149
TseI	GCWGC	6 cut(s)	557, 1099, 2203, 2807, 2920, 2960
Tsp45I	GTSAC	2 cut(s)	1537, 2149
TspDTI	ATGAA	7 cut(s)	828, 930, 1326, 1536, 1743, 2676, 2742
TspGWI	ACGGA	3 cut(s)	1240, 2594, 3027
Vha464I	CTTAAG	2 cut(s)	1277, 2066
VpaK11BI	GGWCC	4 cut(s)	318, 2297, 2793, 2968
XapI	RAATTY	5 cut(s)	10, 1024, 1332, 2443, 2639
XbaI	TCTAGA	3 cut(s)	1768, 2224, 3205
XceI	RCATGY	1 cut(s)	2399
XhoI	CTCGAG	1 cut(s)	1356
XmaJI	CCTAGG	1 cut(s)	115
XmiI	GTMKAC	3 cut(s)	848, 950, 3240
XmnI	GAANNNNTTC	5 cut(s)	1045, 2184, 2422, 2559, 3019
XspI	CTAG	8 cut(s)	116, 378, 1146, 1635, 1769, 1907, 2225, 3206
ZrmI	AGTACT	1 cut(s)	3181
Zsp2I	ATGCAT	1 cut(s)	3107

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.