MD10G1102300.v1.1

Prolyl 4-hydroxylase alpha subunit homologues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010783
Physical Location & Seq
Forward (+)
16695725 .. 16698915
3191 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1102300.v1.1.491

Sequence Viewer

Length: 780 bp
ATGAAAGTGAAAGCAAAGAAGTCCAGAGAAAAGTTGGGCTTACCCACCGTCTTCCTCGTCCGCTCCCTCTTCTTCTTCGCCGGCTTATTCATCTCCACCCTCCTCTCCCATCCCTCCGTCCCTCTCTTCCTCTCCGGCTCTCGACCTGTCTCCGGAACGCTGGAATCGGAAGACGACGAAGATCACGGTCCAATACTGCAGGGTGACACCGGAGAGTGGAGACAGCTTTATCCAGTCGATTCCATTCCAGGTTTTGAGTTGGAGACCGCGCGCTCTGTATTTTCCGAGATTTGCAACTGCGGAGCAGTGGCGAAGATGAAGCTCCGGCCGTCCACATTGGCATTGCGAAAGGGAGAGACTGCCGAGAGCACAAAGGGGGCTCGAACAAGCGCATCAGAAGACGATAGTGGAGTCCTGGATATTATTGAGGAAAATATAGCCAGGGAAACAATGTTACCAAGAACCCATGGCGAGGCATTCAACGTTTTGCGGTATGAGATTGGCCAAAACTACGATTCTCATTATGATGCATTCAATCCAACGGAATATGGCCAACAGAAAAGCCAAAGATTTGCTTCCTTCTTGTTGTATCTGTCTAATGTTGAAGAAGGTGGAGAAACCAATGGTGCAGAGATGGGTATGAGCTATGATTACAGGAAATGCATCGGTTTAAAAATTATGCCGAAGCAAGGGGATGGACTTTTATTTTATTCCATGTTTCCAAATGGGACAATAGATCCGACATTTCTTCACGGAAGCTGCCCTGTAAGCAAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 map00330 map01100
Pfam Domains
Protein Families
2OG-FeII_Oxy_3

Protein Analysis

260

Amino Acids

28.59

Weight (kDa)

5.35

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_3 PF13640 161 - 258 6.5e-11 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010783)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33910
fragaria_vesca FvH4_2g03890 FvH4_2g03890
malus_domestica MD05G1096100.v1.1 MD10G1102300.v1.1
prunus_persica Prupe.8G140500_v2.0.a1
pyrus_communis pycom05g09490 pycom10g08800
rosa_chinensis RchiOBHm_Chr6g0252911
rosa_laevigata RLG00000015049 RLG00000032764
rosa_multiflora Rmu_co8070424.1_g000001 Rmu_sc0000829.1_g000001
rosa_roxburghii Rroxscaffold_7G00212300
rosa_rugosa Rorug05G0540000
rosa_samantha Rh6AG055700 Rh6BG050000 Rh6CG048400 Rh6DG044000
rosa_wichuraiana Rw6G005040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 63
AccII CGCG 2 cut(s) 269, 271
AccIII TCCGGA 1 cut(s) 152
AciI CCGC 4 cut(s) 61, 267, 300, 490
AclI AACGTT 1 cut(s) 483
AclWI GGATC 1 cut(s) 731
AcoI YGGCCR 3 cut(s) 326, 502, 550
AfiI CCNNNNNNNGG 4 cut(s) 152, 216, 472, 689
AgsI TTSAA 3 cut(s) 481, 535, 605
AjnI CCWGG 3 cut(s) 247, 414, 440
AluBI AGCT 4 cut(s) 226, 322, 645, 759
AluI AGCT 4 cut(s) 226, 322, 645, 759
Alw21I GWGCWC 1 cut(s) 371
Alw26I GTCTC 4 cut(s) 154, 214, 257, 350
AlwI GGATC 1 cut(s) 731
Aor13HI TCCGGA 1 cut(s) 152
AoxI GGCC 3 cut(s) 326, 502, 550
ApeKI GCWGC 1 cut(s) 759
AspLEI GCGC 3 cut(s) 271, 273, 392
AspS9I GGNCC 1 cut(s) 188
AsuHPI GGTGA 1 cut(s) 215
AvaII GGWCC 1 cut(s) 188
BalI TGGCCA 2 cut(s) 504, 552
BanII GRGCYC 1 cut(s) 382
BbsI GAAGAC 3 cut(s) 43, 177, 405
Bbv12I GWGCWC 1 cut(s) 371
BbvI GCAGC 1 cut(s) 746
BccI CCATC 3 cut(s) 117, 628, 689
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 3 cut(s) 249, 416, 442
BcoDI GTCTC 4 cut(s) 154, 214, 257, 350
BfmI CTRYAG 1 cut(s) 197
BisI GCNGC 1 cut(s) 760
BlsI GCNGC 1 cut(s) 761
Bme1390I CCNGG 3 cut(s) 249, 416, 442
Bme18I GGWCC 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 188
BmrFI CCNGG 3 cut(s) 249, 416, 442
BmsI GCATC 3 cut(s) 401, 517, 672
BpiI GAAGAC 3 cut(s) 43, 177, 405
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 2 cut(s) 441, 466
BsaWI WCCGGW 2 cut(s) 152, 209
Bsc4I CCNNNNNNNGG 4 cut(s) 152, 216, 472, 689
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 1 cut(s) 233
Bse3DI GCAATG 1 cut(s) 341
BseAI TCCGGA 1 cut(s) 152
BseBI CCWGG 3 cut(s) 249, 416, 442
BseDI CCNNGG 2 cut(s) 441, 466
BseGI GGATG 2 cut(s) 109, 700
BseLI CCNNNNNNNGG 4 cut(s) 152, 216, 472, 689
BseMI GCAATG 1 cut(s) 341
BseNI ACTGG 1 cut(s) 233
BsePI GCGCGC 1 cut(s) 269
BseRI GAGGAG 1 cut(s) 92
BseX3I CGGCCG 1 cut(s) 326
BseXI GCAGC 1 cut(s) 746
BsgI GTGCAG 1 cut(s) 648
Bsh1236I CGCG 2 cut(s) 269, 271
Bsh1285I CGRYCG 1 cut(s) 329
BshFI GGCC 3 cut(s) 328, 504, 552
BsiEI CGRYCG 1 cut(s) 329
BsiHKAI GWGCWC 1 cut(s) 371
BsiSI CCGG 5 cut(s) 81, 135, 153, 210, 325
BslFI GGGAC 2 cut(s) 104, 742
BslI CCNNNNNNNGG 4 cut(s) 152, 216, 472, 689
BsmAI GTCTC 4 cut(s) 154, 214, 257, 350
BsmFI GGGAC 2 cut(s) 104, 742
BsmI GAATGC 2 cut(s) 476, 530
BsnI GGCC 3 cut(s) 328, 504, 552
Bso31I GGTCTC 1 cut(s) 257
Bsp1286I GDGCHC 2 cut(s) 371, 382
Bsp13I TCCGGA 1 cut(s) 152
Bsp143I GATC 2 cut(s) 181, 736
Bsp19I CCATGG 1 cut(s) 466
BspACI CCGC 4 cut(s) 61, 267, 300, 490
BspANI GGCC 3 cut(s) 328, 504, 552
BspEI TCCGGA 1 cut(s) 152
BspFNI CGCG 2 cut(s) 269, 271
BspMAI CTGCAG 1 cut(s) 201
BspPI GGATC 1 cut(s) 731
BspTNI GGTCTC 1 cut(s) 257
BsrBI CCGCTC 1 cut(s) 63
BsrDI GCAATG 1 cut(s) 341
BsrFI RCCGGY 1 cut(s) 80
BsrI ACTGG 1 cut(s) 233
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 2 cut(s) 441, 466
BssHII GCGCGC 1 cut(s) 269
BssMI GATC 2 cut(s) 181, 736
BssT1I CCWWGG 1 cut(s) 466
Bst2UI CCWGG 3 cut(s) 249, 416, 442
Bst4CI ACNGT 2 cut(s) 49, 188
Bst6I CTCTTC 2 cut(s) 74, 131
BstC8I GCNNGC 2 cut(s) 82, 271
BstDSI CCRYGG 1 cut(s) 466
BstF5I GGATG 2 cut(s) 109, 700
BstFNI CGCG 2 cut(s) 269, 271
BstHHI GCGC 3 cut(s) 271, 273, 392
BstKTI GATC 2 cut(s) 184, 739
BstMAI GTCTC 4 cut(s) 154, 214, 257, 350
BstMBI GATC 2 cut(s) 181, 736
BstMCI CGRYCG 1 cut(s) 329
BstMWI GCNNNNNNNGC 1 cut(s) 768
BstNI CCWGG 3 cut(s) 249, 416, 442
BstSCI CCNGG 3 cut(s) 247, 414, 440
BstSFI CTRYAG 1 cut(s) 197
BstUI CGCG 2 cut(s) 269, 271
BstV1I GCAGC 1 cut(s) 746
BstV2I GAAGAC 3 cut(s) 43, 177, 405
BstX2I RGATCY 1 cut(s) 736
BstYI RGATCY 1 cut(s) 736
BstZI CGGCCG 1 cut(s) 326
BsuRI GGCC 3 cut(s) 328, 504, 552
BtgI CCRYGG 1 cut(s) 466
BtsCI GGATG 2 cut(s) 109, 700
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 1 cut(s) 312
Cac8I GCNNGC 2 cut(s) 82, 271
CfoI GCGC 3 cut(s) 271, 273, 392
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 1 cut(s) 188
CviAII CATG 2 cut(s) 467, 715
DpnI GATC 2 cut(s) 183, 738
DpnII GATC 2 cut(s) 181, 736
DraI TTTAAA 1 cut(s) 672
EaeI YGGCCR 3 cut(s) 326, 502, 550
EagI CGGCCG 1 cut(s) 326
Eam1104I CTCTTC 2 cut(s) 74, 131
EarI CTCTTC 2 cut(s) 74, 131
EclXI CGGCCG 1 cut(s) 326
Eco130I CCWWGG 1 cut(s) 466
Eco24I GRGCYC 1 cut(s) 382
Eco31I GGTCTC 1 cut(s) 257
Eco47I GGWCC 1 cut(s) 188
Eco52I CGGCCG 1 cut(s) 326
EcoRII CCWGG 3 cut(s) 247, 414, 440
EcoT14I CCWWGG 1 cut(s) 466
EcoT22I ATGCAT 2 cut(s) 532, 665
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 466
FaeI CATG 2 cut(s) 470, 718
FaiI YATR 9 cut(s) 437, 468, 495, 525, 549, 641, 648, 680, 716
FalI AAGNNNNNCTT 4 cut(s) 23, 55, 559, 591
FaqI GGGAC 2 cut(s) 104, 742
FatI CATG 2 cut(s) 466, 714
Fnu4HI GCNGC 1 cut(s) 760
FokI GGATG 2 cut(s) 96, 707
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 1 cut(s) 760
GlaI GCGC 3 cut(s) 270, 272, 391
GluI GCNGC 1 cut(s) 760
HaeIII GGCC 3 cut(s) 328, 504, 552
HapII CCGG 5 cut(s) 81, 135, 153, 210, 325
HhaI GCGC 3 cut(s) 271, 273, 392
Hin1II CATG 2 cut(s) 470, 718
Hin6I GCGC 3 cut(s) 269, 271, 390
HinP1I GCGC 3 cut(s) 269, 271, 390
HinfI GANTC 4 cut(s) 164, 239, 411, 515
HpaII CCGG 5 cut(s) 81, 135, 153, 210, 325
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 1 cut(s) 333
Hpy188I TCNGA 4 cut(s) 169, 286, 397, 741
Hpy188III TCNNGA 3 cut(s) 24, 141, 153
Hpy8I GTNNAC 1 cut(s) 333
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 2 cut(s) 589, 602
HpyCH4III ACNGT 2 cut(s) 49, 188
HpyCH4IV ACGT 1 cut(s) 483
HpyCH4V TGCA 5 cut(s) 199, 294, 530, 629, 663
HpyF10VI GCNNNNNNNGC 1 cut(s) 768
HpySE526I ACGT 1 cut(s) 483
Hsp92II CATG 2 cut(s) 470, 718
HspAI GCGC 3 cut(s) 269, 271, 390
Kpn2I TCCGGA 1 cut(s) 152
KroI GCCGGC 1 cut(s) 80
KroNI GCCGGC 1 cut(s) 82
Kzo9I GATC 2 cut(s) 181, 736
LmnI GCTCC 3 cut(s) 68, 302, 327
Lsp1109I GCAGC 1 cut(s) 746
LweI GCATC 3 cut(s) 401, 517, 672
MaeII ACGT 1 cut(s) 483
MaeIII GTNAC 2 cut(s) 203, 453
MalI GATC 2 cut(s) 183, 738
MbiI CCGCTC 1 cut(s) 63
MboI GATC 2 cut(s) 181, 736
MflI RGATCY 1 cut(s) 736
MhlI GDGCHC 2 cut(s) 371, 382
MlsI TGGCCA 2 cut(s) 504, 552
MluCI AATT 1 cut(s) 675
MluNI TGGCCA 2 cut(s) 504, 552
MlyI GAGTC 1 cut(s) 420
MmeI TCCRAC 3 cut(s) 240, 563, 764
MnlI CCTC 9 cut(s) 65, 77, 110, 113, 124, 132, 140, 421, 466
Mox20I TGGCCA 2 cut(s) 504, 552
Mph1103I ATGCAT 2 cut(s) 532, 665
MroI TCCGGA 1 cut(s) 152
MroNI GCCGGC 1 cut(s) 80
MscI TGGCCA 2 cut(s) 504, 552
MseI TTAA 1 cut(s) 671
MslI CAYNNNNRTG 1 cut(s) 525
Msp20I TGGCCA 2 cut(s) 504, 552
MspI CCGG 5 cut(s) 81, 135, 153, 210, 325
MspR9I CCNGG 3 cut(s) 249, 416, 442
Mva1269I GAATGC 2 cut(s) 476, 530
MvaI CCWGG 3 cut(s) 249, 416, 442
MvnI CGCG 2 cut(s) 269, 271
MwoI GCNNNNNNNGC 1 cut(s) 768
NaeI GCCGGC 1 cut(s) 82
NcoI CCATGG 1 cut(s) 466
NdeII GATC 2 cut(s) 181, 736
NgoMIV GCCGGC 1 cut(s) 80
NlaIII CATG 2 cut(s) 470, 718
NmeAIII GCCGAG 1 cut(s) 388
NmuCI GTSAC 1 cut(s) 203
NsiI ATGCAT 2 cut(s) 532, 665
PauI GCGCGC 1 cut(s) 269
PctI GAATGC 2 cut(s) 476, 530
PdiI GCCGGC 1 cut(s) 82
PfeI GAWTC 3 cut(s) 164, 239, 515
PfoI TCCNGGA 1 cut(s) 414
PkrI GCNGC 1 cut(s) 761
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
Psp1406I AACGTT 1 cut(s) 483
Psp6I CCWGG 3 cut(s) 247, 414, 440
PspGI CCWGG 3 cut(s) 247, 414, 440
PspPI GGNCC 1 cut(s) 188
PstI CTGCAG 1 cut(s) 201
PsuI RGATCY 1 cut(s) 736
PteI GCGCGC 1 cut(s) 269
RseI CAYNNNNRTG 1 cut(s) 525
SaqAI TTAA 1 cut(s) 671
SatI GCNGC 1 cut(s) 760
Sau3AI GATC 2 cut(s) 181, 736
Sau96I GGNCC 1 cut(s) 188
SchI GAGTC 1 cut(s) 420
ScrFI CCNGG 3 cut(s) 249, 416, 442
SduI GDGCHC 2 cut(s) 371, 382
SetI ASST 8 cut(s) 148, 228, 253, 324, 486, 613, 647, 761
SfaNI GCATC 3 cut(s) 401, 517, 672
SfcI CTRYAG 1 cut(s) 197
SinI GGWCC 1 cut(s) 188
SmiMI CAYNNNNRTG 1 cut(s) 525
Sse9I AATT 1 cut(s) 675
SsiI CCGC 4 cut(s) 61, 267, 300, 490
StyD4I CCNGG 3 cut(s) 247, 414, 440
StyI CCWWGG 1 cut(s) 466
TaaI ACNGT 2 cut(s) 49, 188
TaiI ACGT 1 cut(s) 486
TaqI TCGA 3 cut(s) 142, 237, 382
TasI AATT 1 cut(s) 675
TfiI GAWTC 3 cut(s) 164, 239, 515
Tru1I TTAA 1 cut(s) 671
Tru9I TTAA 1 cut(s) 671
TscAI CASTG 1 cut(s) 312
TseFI GTSAC 1 cut(s) 203
TseI GCWGC 1 cut(s) 759
Tsp45I GTSAC 1 cut(s) 203
TspDTI ATGAA 3 cut(s) 17, 79, 332
TspGWI ACGGA 3 cut(s) 106, 557, 768
TspRI CASTG 1 cut(s) 312
VpaK11BI GGWCC 1 cut(s) 188
XcmI CCANNNNNNNNNTGG 1 cut(s) 31
Zsp2I ATGCAT 2 cut(s) 532, 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.