Rh6DG044000

Prolyl 4-hydroxylase alpha subunit homologues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0010783
Physical Location & Seq
Forward (+)
4730869 .. 4735311
4443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG044000.1

Sequence Viewer

Length: 654 bp
ATGAAACTGAAAGCAAAAGCCCCCAAAGCAAAGTTGGGGCTACCCACCATCGTCCTCCTCTGTTTCCTCTGCTTCATTGCCGGCCTTTTCACCTCCACCCTCCTCTCCCAGGTCTCCGTCCCTCAATTCCTCTCCGGTCCACGGTCGAGTTCTAGAATACTCGAATCGGAGGACGGAGATCACGGTCCGATGCTGCCCGGGGAGACCGGAGAGAGCTTCATTGCGTCGATTCCGTTCCAGGCTAGTAATCTGATCAGGGAGGTGTTGAGTTGGAAGCCGCGGGCGCTTTATTTTCCGAGATTTGCGACTGCGGAGCAATGTGAGAGTGTGATCAAGAGGGCCAAGGCTAAGCTGAGGCCTTCGACGTTGGCTTTGAGGAAAGGAGAGACTGCTGAGAGCACAAAGGGGACTAGAACAAGTTCAGGGACCTTTATCAGTGCATCAGAAGATGAGACCGGAGTCCTGGATATTATTGAGCAAAAGATTGCCCGGGCTACGATGTTACCAAGGGACCATGGAGAGGCATTCAATATATTGCGGTATGACATTGGCCAGAAGTATGATTCTCATTATGATGCGTTCAACCCAGATGAATATGGCCCACAGCAAAGCCAAAGAGTAATAATCCTGTACTTTTACCCTAAATATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 map00330 map01100
Pfam Domains
Protein Families
2OG-FeII_Oxy_3

Protein Analysis

217

Amino Acids

24.15

Weight (kDa)

8.91

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010783)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33910
fragaria_vesca FvH4_2g03890 FvH4_2g03890
malus_domestica MD05G1096100.v1.1 MD10G1102300.v1.1
prunus_persica Prupe.8G140500_v2.0.a1
pyrus_communis pycom05g09490 pycom10g08800
rosa_chinensis RchiOBHm_Chr6g0252911
rosa_laevigata RLG00000015049 RLG00000032764
rosa_multiflora Rmu_co8070424.1_g000001 Rmu_sc0000829.1_g000001
rosa_roxburghii Rroxscaffold_7G00212300
rosa_rugosa Rorug05G0540000
rosa_samantha Rh6AG055700 Rh6BG050000 Rh6CG048400 Rh6DG044000
rosa_wichuraiana Rw6G005040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 280
AciI CCGC 4 cut(s) 278, 280, 311, 538
AcoI YGGCCR 1 cut(s) 550
AfaI GTAC 1 cut(s) 632
AfiI CCNNNNNNNGG 3 cut(s) 109, 141, 520
AgsI TTSAA 2 cut(s) 529, 583
AjnI CCWGG 3 cut(s) 108, 237, 462
AluBI AGCT 2 cut(s) 216, 352
AluI AGCT 2 cut(s) 216, 352
Alw21I GWGCWC 1 cut(s) 401
Alw26I GTCTC 4 cut(s) 118, 197, 380, 446
Ama87I CYCGRG 2 cut(s) 197, 489
AoxI GGCC 5 cut(s) 82, 339, 356, 550, 598
ApeKI GCWGC 1 cut(s) 193
ArsI GACNNNNNNTTYG 2 cut(s) 355, 387
Asp700I GAANNNNTTC 1 cut(s) 418
AspLEI GCGC 1 cut(s) 286
AspS9I GGNCC 6 cut(s) 137, 185, 339, 426, 511, 599
AsuC2I CCSGG 4 cut(s) 198, 199, 490, 491
AsuHPI GGTGA 1 cut(s) 82
AvaI CYCGRG 2 cut(s) 197, 489
AvaII GGWCC 4 cut(s) 137, 185, 426, 511
BalI TGGCCA 1 cut(s) 552
Bbv12I GWGCWC 1 cut(s) 401
BbvCI CCTCAGC 1 cut(s) 353
BbvI GCAGC 1 cut(s) 180
BccI CCATC 1 cut(s) 56
BciT130I CCWGG 3 cut(s) 110, 239, 464
BclI TGATCA 2 cut(s) 252, 330
BcnI CCSGG 4 cut(s) 198, 199, 490, 491
BcoDI GTCTC 4 cut(s) 118, 197, 380, 446
BfaI CTAG 3 cut(s) 153, 243, 411
BfoI RGCGCY 1 cut(s) 287
BisI GCNGC 2 cut(s) 194, 278
BlpI GCTNAGC 1 cut(s) 348
BlsI GCNGC 2 cut(s) 195, 279
Bme1390I CCNGG 7 cut(s) 110, 198, 199, 239, 464, 490, 491
Bme18I GGWCC 4 cut(s) 137, 185, 426, 511
BmeT110I CYCGRG 2 cut(s) 197, 489
BmgT120I GGNCC 6 cut(s) 137, 185, 339, 426, 511, 599
BmiI GGNNCC 2 cut(s) 427, 512
BmrFI CCNGG 7 cut(s) 110, 198, 199, 239, 464, 490, 491
BmsI GCATC 3 cut(s) 180, 449, 565
BoxI GACNNNNGTC 1 cut(s) 458
Bpu10I CCTNAGC 1 cut(s) 353
Bpu1102I GCTNAGC 1 cut(s) 348
BpuMI CCSGG 4 cut(s) 198, 199, 490, 491
BsaI GGTCTC 3 cut(s) 118, 197, 446
BsaJI CCNNGG 9 cut(s) 108, 140, 197, 198, 278, 342, 489, 506, 514
BsaWI WCCGGW 3 cut(s) 134, 206, 455
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 141, 520
Bse118I RCCGGY 1 cut(s) 80
Bse3DI GCAATG 3 cut(s) 75, 219, 323
BseBI CCWGG 3 cut(s) 110, 239, 464
BseDI CCNNGG 9 cut(s) 108, 140, 197, 198, 278, 342, 489, 506, 514
BseLI CCNNNNNNNGG 3 cut(s) 109, 141, 520
BseMI GCAATG 3 cut(s) 75, 219, 323
BseMII CTCAG 2 cut(s) 344, 384
BseRI GAGGAG 2 cut(s) 47, 92
BseXI GCAGC 1 cut(s) 180
Bsh1236I CGCG 1 cut(s) 280
Bsh1285I CGRYCG 1 cut(s) 146
BshFI GGCC 5 cut(s) 84, 341, 358, 552, 600
BsiEI CGRYCG 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 401
BsiHKCI CYCGRG 2 cut(s) 197, 489
BsiSI CCGG 6 cut(s) 81, 135, 198, 207, 456, 490
BslFI GGGAC 4 cut(s) 104, 421, 439, 524
BslI CCNNNNNNNGG 3 cut(s) 109, 141, 520
BsmAI GTCTC 4 cut(s) 118, 197, 380, 446
BsmFI GGGAC 4 cut(s) 104, 421, 439, 524
BsmI GAATGC 1 cut(s) 524
BsnI GGCC 5 cut(s) 84, 341, 358, 552, 600
Bso31I GGTCTC 3 cut(s) 118, 197, 446
BsoBI CYCGRG 2 cut(s) 197, 489
Bsp1286I GDGCHC 1 cut(s) 401
Bsp143I GATC 3 cut(s) 178, 252, 330
Bsp1720I GCTNAGC 1 cut(s) 348
Bsp19I CCATGG 1 cut(s) 514
BspACI CCGC 4 cut(s) 278, 280, 311, 538
BspANI GGCC 5 cut(s) 84, 341, 358, 552, 600
BspCNI CTCAG 2 cut(s) 345, 385
BspFNI CGCG 1 cut(s) 280
BspLI GGNNCC 2 cut(s) 427, 512
BspTNI GGTCTC 3 cut(s) 118, 197, 446
BsrDI GCAATG 3 cut(s) 75, 219, 323
BsrFI RCCGGY 1 cut(s) 80
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 9 cut(s) 108, 140, 197, 198, 278, 342, 489, 506, 514
BssMI GATC 3 cut(s) 178, 252, 330
BssT1I CCWWGG 3 cut(s) 342, 506, 514
Bst2UI CCWGG 3 cut(s) 110, 239, 464
Bst4CI ACNGT 2 cut(s) 144, 185
BstC8I GCNNGC 2 cut(s) 82, 282
BstDEI CTNAG 3 cut(s) 348, 353, 393
BstDSI CCRYGG 3 cut(s) 140, 278, 514
BstENI CCTNNNNNAGG 1 cut(s) 107
BstFNI CGCG 1 cut(s) 280
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 1 cut(s) 286
BstKTI GATC 3 cut(s) 181, 255, 333
BstMAI GTCTC 4 cut(s) 118, 197, 380, 446
BstMBI GATC 3 cut(s) 178, 252, 330
BstMCI CGRYCG 1 cut(s) 146
BstMWI GCNNNNNNNGC 2 cut(s) 26, 283
BstNI CCWGG 3 cut(s) 110, 239, 464
BstPAI GACNNNNGTC 1 cut(s) 458
BstSCI CCNGG 7 cut(s) 108, 196, 197, 237, 462, 488, 489
BstUI CGCG 1 cut(s) 280
BstV1I GCAGC 1 cut(s) 180
BsuRI GGCC 5 cut(s) 84, 341, 358, 552, 600
BtgI CCRYGG 3 cut(s) 140, 278, 514
BtsIMutI CAGTG 1 cut(s) 442
Cac8I GCNNGC 2 cut(s) 82, 282
CfoI GCGC 1 cut(s) 286
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 6 cut(s) 137, 185, 339, 426, 511, 599
Cfr42I CCGCGG 1 cut(s) 281
Cfr9I CCCGGG 2 cut(s) 197, 489
CpoI CGGWCCG 1 cut(s) 185
CseI GACGC 1 cut(s) 213
Csp6I GTAC 1 cut(s) 631
CspI CGGWCCG 1 cut(s) 185
CviAII CATG 1 cut(s) 515
CviQI GTAC 1 cut(s) 631
DdeI CTNAG 3 cut(s) 348, 353, 393
DpnI GATC 3 cut(s) 180, 254, 332
DpnII GATC 3 cut(s) 178, 252, 330
EaeI YGGCCR 1 cut(s) 550
Eco130I CCWWGG 3 cut(s) 342, 506, 514
Eco147I AGGCCT 1 cut(s) 358
Eco31I GGTCTC 3 cut(s) 118, 197, 446
Eco47I GGWCC 4 cut(s) 137, 185, 426, 511
Eco88I CYCGRG 2 cut(s) 197, 489
EcoNI CCTNNNNNAGG 1 cut(s) 107
EcoO109I RGGNCCY 1 cut(s) 426
EcoRII CCWGG 3 cut(s) 108, 237, 462
EcoT14I CCWWGG 3 cut(s) 342, 506, 514
ErhI CCWWGG 3 cut(s) 342, 506, 514
FaeI CATG 1 cut(s) 518
FaiI YATR 8 cut(s) 516, 533, 543, 561, 573, 597, 648, 650
FaqI GGGAC 4 cut(s) 104, 421, 439, 524
FatI CATG 1 cut(s) 514
FauI CCCGC 1 cut(s) 273
FbaI TGATCA 2 cut(s) 252, 330
Fnu4HI GCNGC 2 cut(s) 194, 278
Fsp4HI GCNGC 2 cut(s) 194, 278
FspBI CTAG 3 cut(s) 153, 243, 411
GlaI GCGC 1 cut(s) 285
GluI GCNGC 2 cut(s) 194, 278
HaeII RGCGCY 1 cut(s) 287
HaeIII GGCC 5 cut(s) 84, 341, 358, 552, 600
HapII CCGG 6 cut(s) 81, 135, 198, 207, 456, 490
HgaI GACGC 1 cut(s) 213
HhaI GCGC 1 cut(s) 286
Hin1II CATG 1 cut(s) 518
Hin6I GCGC 1 cut(s) 284
HinP1I GCGC 1 cut(s) 284
HinfI GANTC 4 cut(s) 164, 229, 459, 563
HpaII CCGG 6 cut(s) 81, 135, 198, 207, 456, 490
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 1 cut(s) 140
Hpy188I TCNGA 5 cut(s) 169, 189, 252, 297, 445
Hpy188III TCNNGA 2 cut(s) 153, 334
Hpy8I GTNNAC 1 cut(s) 140
Hpy99I CGWCG 2 cut(s) 229, 367
HpyAV CCTTC 1 cut(s) 369
HpyCH4III ACNGT 2 cut(s) 144, 185
HpyCH4IV ACGT 1 cut(s) 365
HpyCH4V TGCA 1 cut(s) 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 283
HpyF3I CTNAG 3 cut(s) 348, 353, 393
HpySE526I ACGT 1 cut(s) 365
Hsp92II CATG 1 cut(s) 518
HspAI GCGC 1 cut(s) 284
KroI GCCGGC 1 cut(s) 80
KroNI GCCGGC 1 cut(s) 82
Ksp22I TGATCA 2 cut(s) 252, 330
KspI CCGCGG 1 cut(s) 281
Kzo9I GATC 3 cut(s) 178, 252, 330
LmnI GCTCC 1 cut(s) 313
Lsp1109I GCAGC 1 cut(s) 180
LweI GCATC 3 cut(s) 180, 449, 565
MaeI CTAG 3 cut(s) 153, 243, 411
MaeII ACGT 1 cut(s) 365
MaeIII GTNAC 1 cut(s) 501
MalI GATC 3 cut(s) 180, 254, 332
MboI GATC 3 cut(s) 178, 252, 330
MboII GAAGA 1 cut(s) 458
MhlI GDGCHC 1 cut(s) 401
MlsI TGGCCA 1 cut(s) 552
MluCI AATT 1 cut(s) 125
MluNI TGGCCA 1 cut(s) 552
MlyI GAGTC 1 cut(s) 468
MmeI TCCRAC 1 cut(s) 251
Mox20I TGGCCA 1 cut(s) 552
MroNI GCCGGC 1 cut(s) 80
MroXI GAANNNNTTC 1 cut(s) 418
MscI TGGCCA 1 cut(s) 552
MslI CAYNNNNRTG 1 cut(s) 573
Msp20I TGGCCA 1 cut(s) 552
MspA1I CMGCKG 1 cut(s) 280
MspI CCGG 6 cut(s) 81, 135, 198, 207, 456, 490
MspR9I CCNGG 7 cut(s) 110, 198, 199, 239, 464, 490, 491
Mva1269I GAATGC 1 cut(s) 524
MvaI CCWGG 3 cut(s) 110, 239, 464
MvnI CGCG 1 cut(s) 280
MwoI GCNNNNNNNGC 2 cut(s) 26, 283
NaeI GCCGGC 1 cut(s) 82
NciI CCSGG 4 cut(s) 198, 199, 490, 491
NcoI CCATGG 1 cut(s) 514
NdeII GATC 3 cut(s) 178, 252, 330
NgoMIV GCCGGC 1 cut(s) 80
NlaIII CATG 1 cut(s) 518
NlaIV GGNNCC 2 cut(s) 427, 512
PceI AGGCCT 1 cut(s) 358
PctI GAATGC 1 cut(s) 524
PdiI GCCGGC 1 cut(s) 82
PdmI GAANNNNTTC 1 cut(s) 418
PfeI GAWTC 3 cut(s) 164, 229, 563
PfoI TCCNGGA 1 cut(s) 462
PkrI GCNGC 2 cut(s) 195, 279
PleI GAGTC 1 cut(s) 467
PpsI GAGTC 1 cut(s) 467
PpuMI RGGWCCY 1 cut(s) 426
PshAI GACNNNNGTC 1 cut(s) 458
Psp5II RGGWCCY 1 cut(s) 426
Psp6I CCWGG 3 cut(s) 108, 237, 462
PspGI CCWGG 3 cut(s) 108, 237, 462
PspN4I GGNNCC 2 cut(s) 427, 512
PspPI GGNCC 6 cut(s) 137, 185, 339, 426, 511, 599
PspPPI RGGWCCY 1 cut(s) 426
RsaI GTAC 1 cut(s) 632
RsaNI GTAC 1 cut(s) 631
RseI CAYNNNNRTG 1 cut(s) 573
Rsr2I CGGWCCG 1 cut(s) 185
RsrII CGGWCCG 1 cut(s) 185
SacII CCGCGG 1 cut(s) 281
SatI GCNGC 2 cut(s) 194, 278
Sau3AI GATC 3 cut(s) 178, 252, 330
Sau96I GGNCC 6 cut(s) 137, 185, 339, 426, 511, 599
SchI GAGTC 1 cut(s) 468
ScrFI CCNGG 7 cut(s) 110, 198, 199, 239, 464, 490, 491
SduI GDGCHC 1 cut(s) 401
SetI ASST 7 cut(s) 95, 114, 218, 264, 354, 368, 431
SfaNI GCATC 3 cut(s) 180, 449, 565
Sfr303I CCGCGG 1 cut(s) 281
SgrBI CCGCGG 1 cut(s) 281
SinI GGWCC 4 cut(s) 137, 185, 426, 511
SmaI CCCGGG 2 cut(s) 199, 491
SmiMI CAYNNNNRTG 1 cut(s) 573
SrfI GCCCGGGC 1 cut(s) 491
Sse9I AATT 1 cut(s) 125
SseBI AGGCCT 1 cut(s) 358
SsiI CCGC 4 cut(s) 278, 280, 311, 538
SspMI CTAG 3 cut(s) 153, 243, 411
StuI AGGCCT 1 cut(s) 358
StyD4I CCNGG 7 cut(s) 108, 196, 197, 237, 462, 488, 489
StyI CCWWGG 3 cut(s) 342, 506, 514
TaaI ACNGT 2 cut(s) 144, 185
TaiI ACGT 1 cut(s) 368
TaqI TCGA 4 cut(s) 146, 162, 227, 362
TasI AATT 1 cut(s) 125
TatI WGTACW 1 cut(s) 630
TauI GCSGC 1 cut(s) 280
TfiI GAWTC 3 cut(s) 164, 229, 563
TscAI CASTG 1 cut(s) 442
TseI GCWGC 1 cut(s) 193
TspDTI ATGAA 4 cut(s) 17, 64, 208, 606
TspGWI ACGGA 3 cut(s) 106, 189, 222
TspMI CCCGGG 2 cut(s) 197, 489
TspRI CASTG 1 cut(s) 442
VpaK11BI GGWCC 4 cut(s) 137, 185, 426, 511
XagI CCTNNNNNAGG 1 cut(s) 107
XbaI TCTAGA 1 cut(s) 152
XcmI CCANNNNNNNNNTGG 1 cut(s) 31
XmaI CCCGGG 2 cut(s) 197, 489
XmnI GAANNNNTTC 1 cut(s) 418
XspI CTAG 3 cut(s) 153, 243, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.