Rroxscaffold_7G00212300

Prolyl 4-hydroxylase alpha subunit homologues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010783
Physical Location & Seq
Reverse (-)
62697529 .. 62701310
3782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00212300.1

Sequence Viewer

Length: 867 bp
ATGAAACTGAAAGCAAAAGCCCCCAAAGCAAAGTTGGGGCTACCCACCATCGTCCTCCTCTGTTTCCTCTGCTTCATTGCCGGCCTTTTCACCTCCACCCTTCTCTCCCAGGTCTCCGTCCCTCAATTCCTCTCCGGTCCACGGTCGAGTTCTAGAATACTCGAATCGGAGGATGAAGATCACGGCCCAATGCTTCCCGGGGAGACCGGAGAGAGCTTCATTGCGTCGATTCCGTTCCAGGTGTTGAGTTGGAAGCCGCGGGCGCTTTATTTTCCGAGATTTGCGACTGCGGAGCAATGTGAGAGTGTAATCAAGAGGGCCAAGGCTAAGCTCAGGCCTTCGACGTTGGCTTTGAGGAAAGGAGAGACTGTTGAGAGCACAAAGGGGACTAGAACAAGTTCAGGGACCTTTATCAGTGCATCAGAAGATGAATCCGGAGTCCTGGATATTATTGAGCAAAAGATTGCCCGGGCTACGATGTTACCAAGGGACCATGGAGAGGCATTCAATATATTGCGGTATGACATTGGCCAGAAGTATGATTCTCATTATGATGCGTTCAACCCAGATGAATATGGCCCACAGCAAAGCCAAAGATTTGCTTCATTTTTGCTGTATCTATCTAATGTAGAAGAAGGTGGAGAAACAATGTTTCCTTTTGAGAACGGTTCAAATATGGATATTAGATATGATTACAAGAAATGCATTGGTCTAAAAGTTAAGCCAAGGCAAGGGGATGGGCTTTTATTTTATTCGGTGCTTCCAAATGGCACAATTGATCAGACATCTCTTCATGGAAGCTGCCCAGTGATCAGAGGGGAGAAATGGGTGGCAACAAAGTGGATTAGAAATCAAGAAGAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 map00330 map01100
Pfam Domains
Protein Families
2OG-FeII_Oxy_3

Protein Analysis

288

Amino Acids

32.16

Weight (kDa)

6.92

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_3 PF13640 170 - 283 1.9e-15 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010783)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33910
fragaria_vesca FvH4_2g03890 FvH4_2g03890
malus_domestica MD05G1096100.v1.1 MD10G1102300.v1.1
prunus_persica Prupe.8G140500_v2.0.a1
pyrus_communis pycom05g09490 pycom10g08800
rosa_chinensis RchiOBHm_Chr6g0252911
rosa_laevigata RLG00000015049 RLG00000032764
rosa_multiflora Rmu_co8070424.1_g000001 Rmu_sc0000829.1_g000001
rosa_roxburghii Rroxscaffold_7G00212300
rosa_rugosa Rorug05G0540000
rosa_samantha Rh6AG055700 Rh6BG050000 Rh6CG048400 Rh6DG044000
rosa_wichuraiana Rw6G005040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 259
AccIII TCCGGA 1 cut(s) 434
AciI CCGC 4 cut(s) 257, 259, 290, 517
AcoI YGGCCR 1 cut(s) 529
AfiI CCNNNNNNNGG 3 cut(s) 141, 499, 731
AgsI TTSAA 3 cut(s) 508, 562, 672
AjnI CCWGG 3 cut(s) 108, 237, 441
AluBI AGCT 3 cut(s) 216, 331, 801
AluI AGCT 3 cut(s) 216, 331, 801
Alw21I GWGCWC 1 cut(s) 380
Alw26I GTCTC 3 cut(s) 118, 197, 359
Ama87I CYCGRG 2 cut(s) 197, 468
Aor13HI TCCGGA 1 cut(s) 434
AoxI GGCC 6 cut(s) 82, 184, 318, 335, 529, 577
ApeKI GCWGC 1 cut(s) 801
ArsI GACNNNNNNTTYG 2 cut(s) 334, 366
Asp700I GAANNNNTTC 1 cut(s) 397
AspLEI GCGC 1 cut(s) 265
AspS9I GGNCC 6 cut(s) 137, 185, 318, 405, 490, 578
AsuC2I CCSGG 4 cut(s) 198, 199, 469, 470
AsuHPI GGTGA 1 cut(s) 82
AvaI CYCGRG 2 cut(s) 197, 468
AvaII GGWCC 3 cut(s) 137, 405, 490
BalI TGGCCA 1 cut(s) 531
Bbv12I GWGCWC 1 cut(s) 380
BbvI GCAGC 1 cut(s) 788
BccI CCATC 2 cut(s) 56, 731
BceAI ACGGC 1 cut(s) 199
BciT130I CCWGG 3 cut(s) 110, 239, 443
BclI TGATCA 2 cut(s) 778, 810
BcnI CCSGG 4 cut(s) 198, 199, 469, 470
BcoDI GTCTC 3 cut(s) 118, 197, 359
BfaI CTAG 2 cut(s) 153, 390
BfoI RGCGCY 1 cut(s) 266
BisI GCNGC 2 cut(s) 257, 802
BlpI GCTNAGC 1 cut(s) 327
BlsI GCNGC 2 cut(s) 258, 803
Bme1390I CCNGG 7 cut(s) 110, 198, 199, 239, 443, 469, 470
Bme18I GGWCC 3 cut(s) 137, 405, 490
BmeT110I CYCGRG 2 cut(s) 197, 468
BmgT120I GGNCC 6 cut(s) 137, 185, 318, 405, 490, 578
BmiI GGNNCC 2 cut(s) 406, 491
BmrFI CCNGG 7 cut(s) 110, 198, 199, 239, 443, 469, 470
BmrI ACTGGG 1 cut(s) 800
BmsI GCATC 2 cut(s) 428, 544
BmuI ACTGGG 1 cut(s) 800
Bpu10I CCTNAGC 1 cut(s) 332
Bpu1102I GCTNAGC 1 cut(s) 327
BpuMI CCSGG 4 cut(s) 198, 199, 469, 470
BsaBI GATNNNNATC 1 cut(s) 177
BsaI GGTCTC 2 cut(s) 118, 197
BsaWI WCCGGW 3 cut(s) 134, 206, 434
BsaXI ACNNNNNCTCC 2 cut(s) 354, 384
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 499, 731
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 1 cut(s) 806
Bse3DI GCAATG 3 cut(s) 75, 219, 302
Bse8I GATNNNNATC 1 cut(s) 177
BseAI TCCGGA 1 cut(s) 434
BseBI CCWGG 3 cut(s) 110, 239, 443
BseGI GGATG 2 cut(s) 178, 742
BseJI GATNNNNATC 1 cut(s) 177
BseLI CCNNNNNNNGG 3 cut(s) 141, 499, 731
BseMI GCAATG 3 cut(s) 75, 219, 302
BseMII CTCAG 1 cut(s) 346
BseNI ACTGG 1 cut(s) 806
BseRI GAGGAG 1 cut(s) 47
BseXI GCAGC 1 cut(s) 788
Bsh1236I CGCG 1 cut(s) 259
Bsh1285I CGRYCG 1 cut(s) 146
BshFI GGCC 6 cut(s) 84, 186, 320, 337, 531, 579
BsiEI CGRYCG 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 380
BsiHKCI CYCGRG 2 cut(s) 197, 468
BsiSI CCGG 6 cut(s) 81, 135, 198, 207, 435, 469
BslFI GGGAC 4 cut(s) 104, 400, 418, 503
BslI CCNNNNNNNGG 3 cut(s) 141, 499, 731
BsmAI GTCTC 3 cut(s) 118, 197, 359
BsmFI GGGAC 4 cut(s) 104, 400, 418, 503
BsmI GAATGC 1 cut(s) 503
BsnI GGCC 6 cut(s) 84, 186, 320, 337, 531, 579
Bso31I GGTCTC 2 cut(s) 118, 197
BsoBI CYCGRG 2 cut(s) 197, 468
Bsp1286I GDGCHC 1 cut(s) 380
Bsp13I TCCGGA 1 cut(s) 434
Bsp143I GATC 3 cut(s) 178, 778, 810
Bsp1720I GCTNAGC 1 cut(s) 327
Bsp19I CCATGG 1 cut(s) 493
BspACI CCGC 4 cut(s) 257, 259, 290, 517
BspANI GGCC 6 cut(s) 84, 186, 320, 337, 531, 579
BspCNI CTCAG 1 cut(s) 345
BspEI TCCGGA 1 cut(s) 434
BspFNI CGCG 1 cut(s) 259
BspLI GGNNCC 2 cut(s) 406, 491
BspTNI GGTCTC 2 cut(s) 118, 197
BsrDI GCAATG 3 cut(s) 75, 219, 302
BsrFI RCCGGY 1 cut(s) 80
BsrI ACTGG 1 cut(s) 806
BssAI RCCGGY 1 cut(s) 80
BssMI GATC 3 cut(s) 178, 778, 810
BssT1I CCWWGG 4 cut(s) 321, 485, 493, 725
Bst2UI CCWGG 3 cut(s) 110, 239, 443
Bst4CI ACNGT 3 cut(s) 144, 370, 668
Bst6I CTCTTC 2 cut(s) 795, 852
BstC8I GCNNGC 2 cut(s) 82, 261
BstDEI CTNAG 2 cut(s) 327, 332
BstDSI CCRYGG 3 cut(s) 140, 257, 493
BstF5I GGATG 2 cut(s) 178, 742
BstFNI CGCG 1 cut(s) 259
BstH2I RGCGCY 1 cut(s) 266
BstHHI GCGC 1 cut(s) 265
BstKTI GATC 3 cut(s) 181, 781, 813
BstMAI GTCTC 3 cut(s) 118, 197, 359
BstMBI GATC 3 cut(s) 178, 778, 810
BstMCI CGRYCG 1 cut(s) 146
BstMWI GCNNNNNNNGC 2 cut(s) 26, 262
BstNI CCWGG 3 cut(s) 110, 239, 443
BstSCI CCNGG 7 cut(s) 108, 196, 197, 237, 441, 467, 468
BstUI CGCG 1 cut(s) 259
BstV1I GCAGC 1 cut(s) 788
BsuRI GGCC 6 cut(s) 84, 186, 320, 337, 531, 579
BtgI CCRYGG 3 cut(s) 140, 257, 493
BtsCI GGATG 2 cut(s) 178, 742
BtsIMutI CAGTG 2 cut(s) 421, 813
Cac8I GCNNGC 2 cut(s) 82, 261
CfoI GCGC 1 cut(s) 265
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 6 cut(s) 137, 185, 318, 405, 490, 578
Cfr42I CCGCGG 1 cut(s) 260
Cfr9I CCCGGG 2 cut(s) 197, 468
CseI GACGC 1 cut(s) 213
CviAII CATG 2 cut(s) 494, 794
DdeI CTNAG 2 cut(s) 327, 332
DpnI GATC 3 cut(s) 180, 780, 812
DpnII GATC 3 cut(s) 178, 778, 810
EaeI YGGCCR 1 cut(s) 529
Eam1104I CTCTTC 2 cut(s) 795, 852
EarI CTCTTC 2 cut(s) 795, 852
Eco130I CCWWGG 4 cut(s) 321, 485, 493, 725
Eco147I AGGCCT 1 cut(s) 337
Eco31I GGTCTC 2 cut(s) 118, 197
Eco47I GGWCC 3 cut(s) 137, 405, 490
Eco88I CYCGRG 2 cut(s) 197, 468
EcoO109I RGGNCCY 1 cut(s) 405
EcoRII CCWGG 3 cut(s) 108, 237, 441
EcoT14I CCWWGG 4 cut(s) 321, 485, 493, 725
EcoT22I ATGCAT 1 cut(s) 707
ErhI CCWWGG 4 cut(s) 321, 485, 493, 725
FaeI CATG 2 cut(s) 497, 797
FaiI YATR 9 cut(s) 495, 512, 522, 540, 552, 576, 677, 690, 795
FalI AAGNNNNNCTT 2 cut(s) 586, 618
FaqI GGGAC 4 cut(s) 104, 400, 418, 503
FatI CATG 2 cut(s) 493, 793
FauI CCCGC 1 cut(s) 252
FbaI TGATCA 2 cut(s) 778, 810
Fnu4HI GCNGC 2 cut(s) 257, 802
FokI GGATG 2 cut(s) 185, 749
Fsp4HI GCNGC 2 cut(s) 257, 802
FspBI CTAG 2 cut(s) 153, 390
GlaI GCGC 1 cut(s) 264
GluI GCNGC 2 cut(s) 257, 802
HaeII RGCGCY 1 cut(s) 266
HaeIII GGCC 6 cut(s) 84, 186, 320, 337, 531, 579
HapII CCGG 6 cut(s) 81, 135, 198, 207, 435, 469
HgaI GACGC 1 cut(s) 213
HhaI GCGC 1 cut(s) 265
Hin1II CATG 2 cut(s) 497, 797
Hin6I GCGC 1 cut(s) 263
HinP1I GCGC 1 cut(s) 263
HinfI GANTC 5 cut(s) 164, 229, 431, 438, 542
HpaII CCGG 6 cut(s) 81, 135, 198, 207, 435, 469
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 1 cut(s) 140
Hpy188I TCNGA 5 cut(s) 169, 276, 424, 783, 815
Hpy188III TCNNGA 4 cut(s) 153, 313, 435, 854
Hpy8I GTNNAC 1 cut(s) 140
Hpy99I CGWCG 2 cut(s) 229, 346
HpyAV CCTTC 3 cut(s) 110, 348, 629
HpyCH4III ACNGT 3 cut(s) 144, 370, 668
HpyCH4IV ACGT 1 cut(s) 344
HpyCH4V TGCA 2 cut(s) 419, 705
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 262
HpyF3I CTNAG 2 cut(s) 327, 332
HpySE526I ACGT 1 cut(s) 344
Hsp92II CATG 2 cut(s) 497, 797
HspAI GCGC 1 cut(s) 263
Kpn2I TCCGGA 1 cut(s) 434
KroI GCCGGC 1 cut(s) 80
KroNI GCCGGC 1 cut(s) 82
Ksp22I TGATCA 2 cut(s) 778, 810
KspI CCGCGG 1 cut(s) 260
Kzo9I GATC 3 cut(s) 178, 778, 810
LmnI GCTCC 1 cut(s) 292
Lsp1109I GCAGC 1 cut(s) 788
LweI GCATC 2 cut(s) 428, 544
MaeI CTAG 2 cut(s) 153, 390
MaeII ACGT 1 cut(s) 344
MaeIII GTNAC 1 cut(s) 480
MalI GATC 3 cut(s) 180, 780, 812
MboI GATC 3 cut(s) 178, 778, 810
MboII GAAGA 4 cut(s) 188, 437, 644, 782
MfeI CAATTG 1 cut(s) 774
MhlI GDGCHC 1 cut(s) 380
MlsI TGGCCA 1 cut(s) 531
MluCI AATT 2 cut(s) 125, 774
MluNI TGGCCA 1 cut(s) 531
MlyI GAGTC 1 cut(s) 447
MmeI TCCRAC 1 cut(s) 230
Mox20I TGGCCA 1 cut(s) 531
Mph1103I ATGCAT 1 cut(s) 707
MroI TCCGGA 1 cut(s) 434
MroNI GCCGGC 1 cut(s) 80
MroXI GAANNNNTTC 1 cut(s) 397
MscI TGGCCA 1 cut(s) 531
MseI TTAA 1 cut(s) 720
MslI CAYNNNNRTG 1 cut(s) 552
Msp20I TGGCCA 1 cut(s) 531
MspA1I CMGCKG 1 cut(s) 259
MspI CCGG 6 cut(s) 81, 135, 198, 207, 435, 469
MspR9I CCNGG 7 cut(s) 110, 198, 199, 239, 443, 469, 470
MunI CAATTG 1 cut(s) 774
Mva1269I GAATGC 1 cut(s) 503
MvaI CCWGG 3 cut(s) 110, 239, 443
MvnI CGCG 1 cut(s) 259
MwoI GCNNNNNNNGC 2 cut(s) 26, 262
NaeI GCCGGC 1 cut(s) 82
NciI CCSGG 4 cut(s) 198, 199, 469, 470
NcoI CCATGG 1 cut(s) 493
NdeII GATC 3 cut(s) 178, 778, 810
NgoMIV GCCGGC 1 cut(s) 80
NlaIII CATG 2 cut(s) 497, 797
NlaIV GGNNCC 2 cut(s) 406, 491
NsiI ATGCAT 1 cut(s) 707
PceI AGGCCT 1 cut(s) 337
PctI GAATGC 1 cut(s) 503
PdiI GCCGGC 1 cut(s) 82
PdmI GAANNNNTTC 1 cut(s) 397
PfeI GAWTC 4 cut(s) 164, 229, 431, 542
PfoI TCCNGGA 1 cut(s) 441
PkrI GCNGC 2 cut(s) 258, 803
PleI GAGTC 1 cut(s) 446
PpsI GAGTC 1 cut(s) 446
PpuMI RGGWCCY 1 cut(s) 405
Psp5II RGGWCCY 1 cut(s) 405
Psp6I CCWGG 3 cut(s) 108, 237, 441
PspGI CCWGG 3 cut(s) 108, 237, 441
PspN4I GGNNCC 2 cut(s) 406, 491
PspPI GGNCC 6 cut(s) 137, 185, 318, 405, 490, 578
PspPPI RGGWCCY 1 cut(s) 405
RseI CAYNNNNRTG 1 cut(s) 552
SacII CCGCGG 1 cut(s) 260
SaqAI TTAA 1 cut(s) 720
SatI GCNGC 2 cut(s) 257, 802
Sau3AI GATC 3 cut(s) 178, 778, 810
Sau96I GGNCC 6 cut(s) 137, 185, 318, 405, 490, 578
SchI GAGTC 1 cut(s) 447
ScrFI CCNGG 7 cut(s) 110, 198, 199, 239, 443, 469, 470
SduI GDGCHC 1 cut(s) 380
SetI ASST 9 cut(s) 95, 114, 218, 243, 333, 347, 410, 640, 803
SfaNI GCATC 2 cut(s) 428, 544
Sfr303I CCGCGG 1 cut(s) 260
SgrBI CCGCGG 1 cut(s) 260
SinI GGWCC 3 cut(s) 137, 405, 490
SmaI CCCGGG 2 cut(s) 199, 470
SmiMI CAYNNNNRTG 1 cut(s) 552
SrfI GCCCGGGC 1 cut(s) 470
Sse9I AATT 2 cut(s) 125, 774
SseBI AGGCCT 1 cut(s) 337
SsiI CCGC 4 cut(s) 257, 259, 290, 517
SspMI CTAG 2 cut(s) 153, 390
StuI AGGCCT 1 cut(s) 337
StyD4I CCNGG 7 cut(s) 108, 196, 197, 237, 441, 467, 468
StyI CCWWGG 4 cut(s) 321, 485, 493, 725
TaaI ACNGT 3 cut(s) 144, 370, 668
TaiI ACGT 1 cut(s) 347
TaqI TCGA 4 cut(s) 146, 162, 227, 341
TasI AATT 2 cut(s) 125, 774
TauI GCSGC 1 cut(s) 259
TfiI GAWTC 4 cut(s) 164, 229, 431, 542
Tru1I TTAA 1 cut(s) 720
Tru9I TTAA 1 cut(s) 720
TscAI CASTG 2 cut(s) 421, 813
TseI GCWGC 1 cut(s) 801
TspDTI ATGAA 8 cut(s) 17, 64, 189, 208, 444, 585, 594, 782
TspGWI ACGGA 2 cut(s) 106, 222
TspMI CCCGGG 2 cut(s) 197, 468
TspRI CASTG 2 cut(s) 421, 813
VpaK11BI GGWCC 3 cut(s) 137, 405, 490
XbaI TCTAGA 1 cut(s) 152
XcmI CCANNNNNNNNNTGG 1 cut(s) 31
XmaI CCCGGG 2 cut(s) 197, 468
XmnI GAANNNNTTC 1 cut(s) 397
XspI CTAG 2 cut(s) 153, 390
Zsp2I ATGCAT 1 cut(s) 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.