Prupe.8G140500_v2.0.a1

Prolyl 4-hydroxylase alpha subunit homologues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0010783
Physical Location & Seq
Forward (+)
15877765 .. 15882184
4420 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G140500.1

Sequence Viewer

Length: 873 bp
ATGAAAGTGAAAGCAAAAAGCCCCAAAGCGAAATTTGGGCTACCCGCAGTCTTCCTCCTCTGTTCCCTCTTCTTCTTCGTTGGCTTGTTCACCTTCACCCTCCTCTCTCATGTCTCCTTCCCTCAATTTCTCTCTGGTCCACGCTCGGTCTCCAGAACGCTTCAATCGGAAGATGGCGAAGATCACGGTCCAATGCCGCAAGGCGAGACCGGAGACAGCTTTATTCAATCGATTCCATTCCAGGTTTTGAGTTGGAAACCGCGGGCCCTTTATTTTCCGAGATTTGCAACTGCGGAGCAATGCGAAAGCGTGATTGAAATGGCGAAGACTAAGCTCAGGCCGTCGGCATTGGCTTTGCGAAAGGGAGAGACTACTGAGAGCACAAAGGGGACTAGAACAAGTTCAGGCACATTTATTAGTGCATCCGAAGATGAAACCGGAATCCTGGAGATTATTGAGGAAAAGATTGCCAGGGCTACAATGTTACCAAGGACCCATGGAGAGGCATTCAATGTTTTGCGGTATGAGATTGGCCAGAAGTATGATTCTCATTATGATGCATTCAACCCATCAGAATATGGTCAACAGAAAAGCCAAAGATTTGCTTCCTTCTTGTTGTATCTTTCTGATGTAGAAGAAGGTGGAGAAACCATGTTCCCTTTTGAGAATGGCTTACATATGGGTATGAGCTATGATTACAAGAAATGCATTGGTTTAAAGGTTATGCCGAGGCAAGGGGATGGACTTTTATTTTATTCAGTGCTTCCAAATGGCACAATTGATCAGACATCTCTTCATGGAAGCTGCCCGGTGATCAAGGGGGAAAAATGGGTTGCAACAAAGTGGATTAGAAATCAAGAAGATCTGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 map00330 map01100
Pfam Domains
Protein Families
2OG-FeII_Oxy_3

Protein Analysis

291

Amino Acids

32.51

Weight (kDa)

6.34

Isoelectric Point (pI)

49.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010783)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33910
fragaria_vesca FvH4_2g03890 FvH4_2g03890
malus_domestica MD05G1096100.v1.1 MD10G1102300.v1.1
prunus_persica Prupe.8G140500_v2.0.a1
pyrus_communis pycom05g09490 pycom10g08800
rosa_chinensis RchiOBHm_Chr6g0252911
rosa_laevigata RLG00000015049 RLG00000032764
rosa_multiflora Rmu_co8070424.1_g000001 Rmu_sc0000829.1_g000001
rosa_roxburghii Rroxscaffold_7G00212300
rosa_rugosa Rorug05G0540000
rosa_samantha Rh6AG055700 Rh6BG050000 Rh6CG048400 Rh6DG044000
rosa_wichuraiana Rw6G005040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 262
AciI CCGC 6 cut(s) 45, 197, 260, 262, 293, 520
AcoI YGGCCR 1 cut(s) 532
AcsI RAATTY 1 cut(s) 32
AfiI CCNNNNNNNGG 2 cut(s) 502, 734
AgsI TTSAA 5 cut(s) 164, 227, 317, 511, 565
AjnI CCWGG 3 cut(s) 240, 444, 470
AluBI AGCT 4 cut(s) 219, 334, 690, 804
AluI AGCT 4 cut(s) 219, 334, 690, 804
Alw21I GWGCWC 1 cut(s) 383
Alw26I GTCTC 5 cut(s) 118, 154, 200, 207, 362
AoxI GGCC 3 cut(s) 264, 338, 532
ApaI GGGCCC 1 cut(s) 268
ApeKI GCWGC 1 cut(s) 804
ApoI RAATTY 1 cut(s) 32
Asp700I GAANNNNTTC 1 cut(s) 400
AspS9I GGNCC 5 cut(s) 137, 188, 264, 265, 492
AsuC2I CCSGG 1 cut(s) 809
AsuHPI GGTGA 3 cut(s) 82, 88, 823
AvaII GGWCC 3 cut(s) 137, 188, 492
BaeGI GKGCMC 1 cut(s) 268
BalI TGGCCA 1 cut(s) 534
BanII GRGCYC 1 cut(s) 268
BbsI GAAGAC 2 cut(s) 43, 332
Bbv12I GWGCWC 1 cut(s) 383
BbvI GCAGC 1 cut(s) 791
BccI CCATC 3 cut(s) 167, 577, 734
BceAI ACGGC 1 cut(s) 325
BciT130I CCWGG 3 cut(s) 242, 446, 472
BclI TGATCA 2 cut(s) 781, 813
BcnI CCSGG 1 cut(s) 809
BcoDI GTCTC 5 cut(s) 118, 154, 200, 207, 362
BfaI CTAG 1 cut(s) 393
BglII AGATCT 1 cut(s) 862
BisI GCNGC 2 cut(s) 197, 805
BlsI GCNGC 2 cut(s) 198, 806
Bme1390I CCNGG 4 cut(s) 242, 446, 472, 809
Bme18I GGWCC 3 cut(s) 137, 188, 492
BmgT120I GGNCC 5 cut(s) 137, 188, 264, 265, 492
BmiI GGNNCC 2 cut(s) 266, 494
BmrFI CCNGG 4 cut(s) 242, 446, 472, 809
BmsI GCATC 2 cut(s) 431, 547
BpiI GAAGAC 2 cut(s) 43, 332
BpmI CTGGAG 2 cut(s) 136, 467
Bpu10I CCTNAGC 1 cut(s) 335
BpuMI CCSGG 1 cut(s) 809
Bsa29I ATCGAT 1 cut(s) 230
BsaI GGTCTC 2 cut(s) 154, 200
BsaJI CCNNGG 5 cut(s) 260, 471, 488, 496, 728
BsaWI WCCGGW 2 cut(s) 209, 437
Bsc4I CCNNNNNNNGG 2 cut(s) 502, 734
Bse3DI GCAATG 1 cut(s) 305
BseBI CCWGG 3 cut(s) 242, 446, 472
BseCI ATCGAT 1 cut(s) 230
BseDI CCNNGG 5 cut(s) 260, 471, 488, 496, 728
BseGI GGATG 2 cut(s) 422, 745
BseLI CCNNNNNNNGG 2 cut(s) 502, 734
BseMI GCAATG 1 cut(s) 305
BseMII CTCAG 2 cut(s) 349, 366
BseRI GAGGAG 2 cut(s) 47, 92
BseSI GKGCMC 1 cut(s) 268
BseXI GCAGC 1 cut(s) 791
Bsh1236I CGCG 1 cut(s) 262
BshFI GGCC 3 cut(s) 266, 340, 534
BshVI ATCGAT 1 cut(s) 230
BsiHKAI GWGCWC 1 cut(s) 383
BsiSI CCGG 3 cut(s) 210, 438, 809
BslFI GGGAC 1 cut(s) 403
BslI CCNNNNNNNGG 2 cut(s) 502, 734
BsmAI GTCTC 5 cut(s) 118, 154, 200, 207, 362
BsmFI GGGAC 1 cut(s) 403
BsmI GAATGC 2 cut(s) 506, 560
BsnI GGCC 3 cut(s) 266, 340, 534
Bso31I GGTCTC 2 cut(s) 154, 200
Bsp120I GGGCCC 1 cut(s) 264
Bsp1286I GDGCHC 2 cut(s) 268, 383
Bsp143I GATC 4 cut(s) 181, 781, 813, 862
Bsp19I CCATGG 1 cut(s) 496
BspACI CCGC 6 cut(s) 45, 197, 260, 262, 293, 520
BspANI GGCC 3 cut(s) 266, 340, 534
BspCNI CTCAG 2 cut(s) 348, 367
BspDI ATCGAT 1 cut(s) 230
BspFNI CGCG 1 cut(s) 262
BspLI GGNNCC 2 cut(s) 266, 494
BspTNI GGTCTC 2 cut(s) 154, 200
BsrDI GCAATG 1 cut(s) 305
BssECI CCNNGG 5 cut(s) 260, 471, 488, 496, 728
BssMI GATC 4 cut(s) 181, 781, 813, 862
BssT1I CCWWGG 2 cut(s) 488, 496
Bst2UI CCWGG 3 cut(s) 242, 446, 472
Bst4CI ACNGT 1 cut(s) 188
Bst6I CTCTTC 2 cut(s) 74, 798
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 3 cut(s) 330, 335, 375
BstDSI CCRYGG 2 cut(s) 260, 496
BstF5I GGATG 2 cut(s) 422, 745
BstFNI CGCG 1 cut(s) 262
BstKTI GATC 4 cut(s) 184, 784, 816, 865
BstMAI GTCTC 5 cut(s) 118, 154, 200, 207, 362
BstMBI GATC 4 cut(s) 181, 781, 813, 862
BstNI CCWGG 3 cut(s) 242, 446, 472
BstSCI CCNGG 4 cut(s) 240, 444, 470, 807
BstSLI GKGCMC 1 cut(s) 268
BstUI CGCG 1 cut(s) 262
BstV1I GCAGC 1 cut(s) 791
BstV2I GAAGAC 2 cut(s) 43, 332
BstX2I RGATCY 1 cut(s) 862
BstYI RGATCY 1 cut(s) 862
Bsu15I ATCGAT 1 cut(s) 230
BsuRI GGCC 3 cut(s) 266, 340, 534
BsuTUI ATCGAT 1 cut(s) 230
BtgI CCRYGG 2 cut(s) 260, 496
BtsCI GGATG 2 cut(s) 422, 745
BtsIMutI CAGTG 1 cut(s) 765
Cac8I GCNNGC 1 cut(s) 264
Cfr13I GGNCC 5 cut(s) 137, 188, 264, 265, 492
Cfr42I CCGCGG 1 cut(s) 263
ClaI ATCGAT 1 cut(s) 230
CviAII CATG 4 cut(s) 110, 497, 652, 797
DdeI CTNAG 3 cut(s) 330, 335, 375
DpnI GATC 4 cut(s) 183, 783, 815, 864
DpnII GATC 4 cut(s) 181, 781, 813, 862
DraI TTTAAA 1 cut(s) 717
EaeI YGGCCR 1 cut(s) 532
Eam1104I CTCTTC 2 cut(s) 74, 798
EarI CTCTTC 2 cut(s) 74, 798
Eco130I CCWWGG 2 cut(s) 488, 496
Eco24I GRGCYC 1 cut(s) 268
Eco31I GGTCTC 2 cut(s) 154, 200
Eco47I GGWCC 3 cut(s) 137, 188, 492
EcoO109I RGGNCCY 2 cut(s) 265, 492
EcoRII CCWGG 3 cut(s) 240, 444, 470
EcoT14I CCWWGG 2 cut(s) 488, 496
EcoT22I ATGCAT 2 cut(s) 562, 710
EcoT38I GRGCYC 1 cut(s) 268
ErhI CCWWGG 2 cut(s) 488, 496
FaeI CATG 4 cut(s) 113, 500, 655, 800
FalI AAGNNNNNCTT 2 cut(s) 589, 621
FaqI GGGAC 1 cut(s) 403
FatI CATG 4 cut(s) 109, 496, 651, 796
FauI CCCGC 2 cut(s) 52, 255
FauNDI CATATG 1 cut(s) 678
FbaI TGATCA 2 cut(s) 781, 813
Fnu4HI GCNGC 2 cut(s) 197, 805
FokI GGATG 2 cut(s) 409, 752
FriOI GRGCYC 1 cut(s) 268
Fsp4HI GCNGC 2 cut(s) 197, 805
FspBI CTAG 1 cut(s) 393
GluI GCNGC 2 cut(s) 197, 805
GsuI CTGGAG 2 cut(s) 136, 467
HaeIII GGCC 3 cut(s) 266, 340, 534
HapII CCGG 3 cut(s) 210, 438, 809
Hin1II CATG 4 cut(s) 113, 500, 655, 800
HincII GTYRAC 1 cut(s) 584
HindII GTYRAC 1 cut(s) 584
HinfI GANTC 3 cut(s) 232, 441, 545
HpaII CCGG 3 cut(s) 210, 438, 809
HphI GGTGA 3 cut(s) 82, 88, 823
Hpy166II GTNNAC 3 cut(s) 90, 140, 584
Hpy188I TCNGA 6 cut(s) 169, 279, 427, 574, 628, 786
Hpy188III TCNNGA 3 cut(s) 153, 857, 866
Hpy8I GTNNAC 3 cut(s) 90, 140, 584
Hpy99I CGWCG 1 cut(s) 346
HpyAV CCTTC 4 cut(s) 103, 127, 619, 632
HpyCH4III ACNGT 1 cut(s) 188
HpyCH4V TGCA 5 cut(s) 287, 422, 560, 708, 836
HpyF3I CTNAG 3 cut(s) 330, 335, 375
Hsp92II CATG 4 cut(s) 113, 500, 655, 800
Ksp22I TGATCA 2 cut(s) 781, 813
KspI CCGCGG 1 cut(s) 263
Kzo9I GATC 4 cut(s) 181, 781, 813, 862
LmnI GCTCC 1 cut(s) 295
Lsp1109I GCAGC 1 cut(s) 791
LweI GCATC 2 cut(s) 431, 547
MaeI CTAG 1 cut(s) 393
MaeIII GTNAC 1 cut(s) 483
MalI GATC 4 cut(s) 183, 783, 815, 864
MboI GATC 4 cut(s) 181, 781, 813, 862
MfeI CAATTG 1 cut(s) 777
MflI RGATCY 1 cut(s) 862
MhlI GDGCHC 2 cut(s) 268, 383
MlsI TGGCCA 1 cut(s) 534
MluCI AATT 3 cut(s) 32, 125, 777
MluNI TGGCCA 1 cut(s) 534
MmeI TCCRAC 1 cut(s) 233
MnlI CCTC 9 cut(s) 65, 68, 77, 110, 113, 132, 451, 496, 723
Mox20I TGGCCA 1 cut(s) 534
Mph1103I ATGCAT 2 cut(s) 562, 710
MroXI GAANNNNTTC 1 cut(s) 400
MscI TGGCCA 1 cut(s) 534
MseI TTAA 1 cut(s) 716
MslI CAYNNNNRTG 1 cut(s) 555
Msp20I TGGCCA 1 cut(s) 534
MspA1I CMGCKG 1 cut(s) 262
MspI CCGG 3 cut(s) 210, 438, 809
MspR9I CCNGG 4 cut(s) 242, 446, 472, 809
MunI CAATTG 1 cut(s) 777
Mva1269I GAATGC 2 cut(s) 506, 560
MvaI CCWGG 3 cut(s) 242, 446, 472
MvnI CGCG 1 cut(s) 262
NciI CCSGG 1 cut(s) 809
NcoI CCATGG 1 cut(s) 496
NdeI CATATG 1 cut(s) 678
NdeII GATC 4 cut(s) 181, 781, 813, 862
NlaIII CATG 4 cut(s) 113, 500, 655, 800
NlaIV GGNNCC 2 cut(s) 266, 494
NmeAIII GCCGAG 1 cut(s) 753
NsiI ATGCAT 2 cut(s) 562, 710
PctI GAATGC 2 cut(s) 506, 560
PdmI GAANNNNTTC 1 cut(s) 400
PfeI GAWTC 3 cut(s) 232, 441, 545
PfoI TCCNGGA 1 cut(s) 444
PkrI GCNGC 2 cut(s) 198, 806
PpuMI RGGWCCY 1 cut(s) 492
Psp5II RGGWCCY 1 cut(s) 492
Psp6I CCWGG 3 cut(s) 240, 444, 470
PspGI CCWGG 3 cut(s) 240, 444, 470
PspN4I GGNNCC 2 cut(s) 266, 494
PspOMI GGGCCC 1 cut(s) 264
PspPI GGNCC 5 cut(s) 137, 188, 264, 265, 492
PspPPI RGGWCCY 1 cut(s) 492
PsuI RGATCY 1 cut(s) 862
RseI CAYNNNNRTG 1 cut(s) 555
SacII CCGCGG 1 cut(s) 263
SaqAI TTAA 1 cut(s) 716
SatI GCNGC 2 cut(s) 197, 805
Sau3AI GATC 4 cut(s) 181, 781, 813, 862
Sau96I GGNCC 5 cut(s) 137, 188, 264, 265, 492
ScrFI CCNGG 4 cut(s) 242, 446, 472, 809
SduI GDGCHC 2 cut(s) 268, 383
SetI ASST 8 cut(s) 95, 221, 246, 336, 643, 692, 723, 806
SfaNI GCATC 2 cut(s) 431, 547
Sfr303I CCGCGG 1 cut(s) 263
SgrBI CCGCGG 1 cut(s) 263
SinI GGWCC 3 cut(s) 137, 188, 492
SmiMI CAYNNNNRTG 1 cut(s) 555
Sse9I AATT 3 cut(s) 32, 125, 777
SsiI CCGC 6 cut(s) 45, 197, 260, 262, 293, 520
SspMI CTAG 1 cut(s) 393
StyD4I CCNGG 4 cut(s) 240, 444, 470, 807
StyI CCWWGG 2 cut(s) 488, 496
TaaI ACNGT 1 cut(s) 188
TaqI TCGA 1 cut(s) 230
TaqII GACCGA 1 cut(s) 136
TasI AATT 3 cut(s) 32, 125, 777
TauI GCSGC 1 cut(s) 199
TfiI GAWTC 3 cut(s) 232, 441, 545
Tru1I TTAA 1 cut(s) 716
Tru9I TTAA 1 cut(s) 716
TscAI CASTG 1 cut(s) 765
TseI GCWGC 1 cut(s) 804
TspDTI ATGAA 3 cut(s) 17, 447, 785
TspRI CASTG 1 cut(s) 765
VpaK11BI GGWCC 3 cut(s) 137, 188, 492
XapI RAATTY 1 cut(s) 32
XmnI GAANNNNTTC 1 cut(s) 400
XspI CTAG 1 cut(s) 393
Zsp2I ATGCAT 2 cut(s) 562, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.