MD10G1152600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006566
Physical Location & Seq
Reverse (-)
24018883 .. 24022357
3475 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1152600.v1.1.491

Sequence Viewer

Length: 774 bp
ATGCTCTCCCTCGCAAAACGACTCAAGAGCTCGCCGTCATGCTTCCAGACTGCGGCCGCCGCCACCCAACTCCGGTGGGCTCGGACCGAGTCCGGCCCATCTCGGCGCCGCCACAAGTCTCCCGCCCTGGCCATGCGGAAGCAAGAGGAGAAGTCCGAGTGGTGGATCGTTGACGGCGAAATCCACGAGATCGGCGATCACGTGCCTCCACGTGAGCGGTTTGTCATTCCCAGGGACAATATTCCCAACAAGCGCCGCAAGCAGCTCCGTGACCAGTTCATGCGCCGCACTCGCCTCGTCCTCAAGGAAACGGAACACGAGCCTTGGTGCAAAAGATACATGGAGCTTTACCAGGAGCTGAGGGAAAATTGGGAGAGGCTGTATTGGGACGAGGGTTATTCCAAAAAAATTGCTCAGGATCATGCGAATTATGAATCCGCTGAGGACGATGATCACGATTTCTCTCCGTACAGGAACAAGCGGTTTCATCCCGAGCCATTTAAGGACAATGCTTTTGCAAGTAGGCAAGGTGATACCTGGGAAAAGGTCAACCAGATTCGGGATAAATTTGAGTATGACAGAGAAAGGAGAATGAGAGAGAAAGCATTTGCACCGATGTCTGGAGAAGCTCCTAACTTCCATGATATGAATTCCCCGAGACAGCCTAACTTCCATGATATGAACTCCCGGAGACAGCCAAATGTCCATGATATGAACTCCCGGAGACAACCATTTGATGCCCAGAAATACTTCTCAGATTCTGAAACAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

258

Amino Acids

31.13

Weight (kDa)

9.11

Isoelectric Point (pI)

71.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 105
AccBSI CCGCTC 1 cut(s) 217
AclWI GGATC 2 cut(s) 173, 426
AcoI YGGCCR 2 cut(s) 54, 129
AcsI RAATTY 2 cut(s) 566, 649
AcvI CACGTG 2 cut(s) 202, 212
AcyI GRCGYC 1 cut(s) 106
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 5 cut(s) 52, 72, 162, 559, 620
AjnI CCWGG 4 cut(s) 126, 230, 351, 536
AluBI AGCT 5 cut(s) 30, 265, 346, 358, 629
AluI AGCT 5 cut(s) 30, 265, 346, 358, 629
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 4 cut(s) 123, 652, 685, 718
AlwI GGATC 2 cut(s) 173, 426
AlwNI CAGNNNCTG 2 cut(s) 358, 761
Ama87I CYCGRG 2 cut(s) 491, 655
AoxI GGCC 3 cut(s) 54, 94, 129
ApeKI GCWGC 1 cut(s) 262
ApoI RAATTY 2 cut(s) 566, 649
ArsI GACNNNNNNTTYG 2 cut(s) 497, 529
Asp700I GAANNNNTTC 1 cut(s) 749
AspLEI GCGC 3 cut(s) 108, 255, 285
AspS9I GGNCC 2 cut(s) 84, 95
AsuC2I CCSGG 2 cut(s) 688, 721
AsuHPI GGTGA 1 cut(s) 542
AvaI CYCGRG 2 cut(s) 491, 655
AvaII GGWCC 1 cut(s) 84
BalI TGGCCA 1 cut(s) 131
BanI GGYRCC 1 cut(s) 105
BanII GRGCYC 2 cut(s) 32, 82
BauI CACGAG 2 cut(s) 185, 317
BbrPI CACGTG 2 cut(s) 202, 212
Bbv12I GWGCWC 1 cut(s) 32
BbvCI CCTCAGC 2 cut(s) 359, 441
BbvI GCAGC 1 cut(s) 274
BccI CCATC 1 cut(s) 106
BceAI ACGGC 2 cut(s) 19, 190
BcgI CGANNNNNNTGC 2 cut(s) 277, 311
BciT130I CCWGG 4 cut(s) 128, 232, 353, 538
BclI TGATCA 1 cut(s) 451
BcnI CCSGG 2 cut(s) 688, 721
BcoDI GTCTC 4 cut(s) 123, 652, 685, 718
BfoI RGCGCY 2 cut(s) 109, 256
BisI GCNGC 7 cut(s) 54, 57, 60, 109, 256, 263, 286
BlsI GCNGC 7 cut(s) 55, 58, 61, 110, 257, 264, 287
Bme1390I CCNGG 6 cut(s) 128, 232, 353, 538, 688, 721
Bme18I GGWCC 1 cut(s) 84
BmeT110I CYCGRG 2 cut(s) 491, 655
BmgT120I GGNCC 2 cut(s) 84, 95
BmiI GGNNCC 1 cut(s) 107
BmrFI CCNGG 6 cut(s) 128, 232, 353, 538, 688, 721
BmsI GCATC 1 cut(s) 727
BpmI CTGGAG 1 cut(s) 642
Bpu10I CCTNAGC 3 cut(s) 359, 414, 441
BpuEI CTTGAG 2 cut(s) 8, 287
BpuMI CCSGG 2 cut(s) 688, 721
BsaAI YACGTR 2 cut(s) 202, 212
BsaHI GRCGYC 1 cut(s) 106
BsaJI CCNNGG 5 cut(s) 126, 230, 231, 323, 537
BsaWI WCCGGW 1 cut(s) 72
BsaXI ACNNNNNCTCC 2 cut(s) 365, 395
Bsc4I CCNNNNNNNGG 5 cut(s) 52, 72, 162, 559, 620
Bse1I ACTGG 1 cut(s) 274
BseBI CCWGG 4 cut(s) 128, 232, 353, 538
BseDI CCNNGG 5 cut(s) 126, 230, 231, 323, 537
BseGI GGATG 1 cut(s) 487
BseLI CCNNNNNNNGG 5 cut(s) 52, 72, 162, 559, 620
BseMII CTCAG 4 cut(s) 350, 428, 432, 768
BseNI ACTGG 1 cut(s) 274
BseRI GAGGAG 1 cut(s) 161
BseX3I CGGCCG 1 cut(s) 54
BseXI GCAGC 1 cut(s) 274
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 3 cut(s) 56, 96, 131
BshNI GGYRCC 1 cut(s) 105
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 2 cut(s) 491, 655
BsiSI CCGG 4 cut(s) 73, 93, 688, 721
BslFI GGGAC 2 cut(s) 248, 401
BslI CCNNNNNNNGG 5 cut(s) 52, 72, 162, 559, 620
BsmAI GTCTC 4 cut(s) 123, 652, 685, 718
BsmFI GGGAC 2 cut(s) 248, 401
BsnI GGCC 3 cut(s) 56, 96, 131
BsoBI CYCGRG 2 cut(s) 491, 655
Bsp1286I GDGCHC 2 cut(s) 32, 82
Bsp143I GATC 5 cut(s) 165, 189, 196, 418, 451
BspANI GGCC 3 cut(s) 56, 96, 131
BspCNI CTCAG 4 cut(s) 351, 427, 433, 767
BspLI GGNNCC 1 cut(s) 107
BspPI GGATC 2 cut(s) 173, 426
BspT107I GGYRCC 1 cut(s) 105
BsrBI CCGCTC 1 cut(s) 217
BsrI ACTGG 1 cut(s) 274
BssECI CCNNGG 5 cut(s) 126, 230, 231, 323, 537
BssMI GATC 5 cut(s) 165, 189, 196, 418, 451
BssNI GRCGYC 1 cut(s) 106
BssSI CACGAG 2 cut(s) 185, 317
BssT1I CCWWGG 1 cut(s) 323
Bst2BI CACGAG 2 cut(s) 185, 317
Bst2UI CCWGG 4 cut(s) 128, 232, 353, 538
BstACI GRCGYC 1 cut(s) 106
BstBAI YACGTR 2 cut(s) 202, 212
BstC8I GCNNGC 2 cut(s) 32, 260
BstDEI CTNAG 4 cut(s) 359, 414, 441, 754
BstF5I GGATG 1 cut(s) 487
BstH2I RGCGCY 2 cut(s) 109, 256
BstHHI GCGC 3 cut(s) 108, 255, 285
BstKTI GATC 5 cut(s) 168, 192, 199, 421, 454
BstMAI GTCTC 4 cut(s) 123, 652, 685, 718
BstMBI GATC 5 cut(s) 165, 189, 196, 418, 451
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 3 cut(s) 59, 259, 291
BstNI CCWGG 4 cut(s) 128, 232, 353, 538
BstSCI CCNGG 6 cut(s) 126, 230, 351, 536, 686, 719
BstV1I GCAGC 1 cut(s) 274
BstZI CGGCCG 1 cut(s) 54
BsuRI GGCC 3 cut(s) 56, 96, 131
BtsCI GGATG 1 cut(s) 487
Cac8I GCNNGC 2 cut(s) 32, 260
CaiI CAGNNNCTG 2 cut(s) 358, 761
CciNI GCGGCCGC 1 cut(s) 54
CfoI GCGC 3 cut(s) 108, 255, 285
Cfr13I GGNCC 2 cut(s) 84, 95
CpoI CGGWCCG 1 cut(s) 84
Csp6I GTAC 1 cut(s) 469
CspCI CAANNNNNGTGG 2 cut(s) 56, 91
CspI CGGWCCG 1 cut(s) 84
CviAII CATG 8 cut(s) 39, 133, 280, 340, 422, 641, 674, 707
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 4 cut(s) 359, 414, 441, 754
DinI GGCGCC 1 cut(s) 107
DpnI GATC 5 cut(s) 167, 191, 198, 420, 453
DpnII GATC 5 cut(s) 165, 189, 196, 418, 451
EaeI YGGCCR 2 cut(s) 54, 129
EagI CGGCCG 1 cut(s) 54
Ecl136II GAGCTC 1 cut(s) 30
EclXI CGGCCG 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 323
Eco24I GRGCYC 2 cut(s) 32, 82
Eco47I GGWCC 1 cut(s) 84
Eco52I CGGCCG 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 30
Eco72I CACGTG 2 cut(s) 202, 212
Eco88I CYCGRG 2 cut(s) 491, 655
EcoICRI GAGCTC 1 cut(s) 30
EcoRI GAATTC 1 cut(s) 649
EcoRII CCWGG 4 cut(s) 126, 230, 351, 536
EcoT14I CCWWGG 1 cut(s) 323
EcoT38I GRGCYC 2 cut(s) 32, 82
EgeI GGCGCC 1 cut(s) 107
EheI GGCGCC 1 cut(s) 107
ErhI CCWWGG 1 cut(s) 323
FaeI CATG 8 cut(s) 42, 136, 283, 343, 425, 644, 677, 710
FaqI GGGAC 2 cut(s) 248, 401
FatI CATG 8 cut(s) 38, 132, 279, 339, 421, 640, 673, 706
FauI CCCGC 1 cut(s) 130
FbaI TGATCA 1 cut(s) 451
Fnu4HI GCNGC 7 cut(s) 54, 57, 60, 109, 256, 263, 286
FokI GGATG 1 cut(s) 474
FriOI GRGCYC 2 cut(s) 32, 82
Fsp4HI GCNGC 7 cut(s) 54, 57, 60, 109, 256, 263, 286
GlaI GCGC 3 cut(s) 107, 254, 284
GluI GCNGC 7 cut(s) 54, 57, 60, 109, 256, 263, 286
GsuI CTGGAG 1 cut(s) 642
HaeII RGCGCY 2 cut(s) 109, 256
HaeIII GGCC 3 cut(s) 56, 96, 131
HapII CCGG 4 cut(s) 73, 93, 688, 721
HhaI GCGC 3 cut(s) 108, 255, 285
Hin1I GRCGYC 1 cut(s) 106
Hin1II CATG 8 cut(s) 42, 136, 283, 343, 425, 644, 677, 710
Hin6I GCGC 3 cut(s) 106, 253, 283
HinP1I GCGC 3 cut(s) 106, 253, 283
HincII GTYRAC 2 cut(s) 172, 550
HindII GTYRAC 2 cut(s) 172, 550
HinfI GANTC 5 cut(s) 21, 89, 434, 556, 758
HpaII CCGG 4 cut(s) 73, 93, 688, 721
HphI GGTGA 1 cut(s) 542
Hpy166II GTNNAC 2 cut(s) 172, 550
Hpy188I TCNGA 4 cut(s) 84, 157, 757, 763
Hpy188III TCNNGA 7 cut(s) 25, 46, 416, 455, 491, 560, 621
Hpy8I GTNNAC 2 cut(s) 172, 550
HpyCH4IV ACGT 2 cut(s) 201, 211
HpyCH4V TGCA 3 cut(s) 330, 518, 611
HpyF10VI GCNNNNNNNGC 3 cut(s) 59, 259, 291
HpyF3I CTNAG 4 cut(s) 359, 414, 441, 754
HpySE526I ACGT 2 cut(s) 201, 211
Hsp92I GRCGYC 1 cut(s) 106
Hsp92II CATG 8 cut(s) 42, 136, 283, 343, 425, 644, 677, 710
HspAI GCGC 3 cut(s) 106, 253, 283
KasI GGCGCC 1 cut(s) 105
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 5 cut(s) 165, 189, 196, 418, 451
LmnI GCTCC 4 cut(s) 270, 343, 355, 634
Lsp1109I GCAGC 1 cut(s) 274
LweI GCATC 1 cut(s) 727
MaeII ACGT 2 cut(s) 201, 211
MaeIII GTNAC 1 cut(s) 269
MalI GATC 5 cut(s) 167, 191, 198, 420, 453
MbiI CCGCTC 1 cut(s) 217
MboI GATC 5 cut(s) 165, 189, 196, 418, 451
MhlI GDGCHC 2 cut(s) 32, 82
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 5 cut(s) 367, 408, 427, 566, 649
MluNI TGGCCA 1 cut(s) 131
Mly113I GGCGCC 1 cut(s) 106
MlyI GAGTC 2 cut(s) 15, 98
MnlI CCTC 9 cut(s) 20, 139, 216, 305, 311, 354, 369, 385, 436
Mox20I TGGCCA 1 cut(s) 131
MroXI GAANNNNTTC 1 cut(s) 749
MscI TGGCCA 1 cut(s) 131
MseI TTAA 1 cut(s) 501
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 4 cut(s) 73, 93, 688, 721
MspR9I CCNGG 6 cut(s) 128, 232, 353, 538, 688, 721
MvaI CCWGG 4 cut(s) 128, 232, 353, 538
MwoI GCNNNNNNNGC 3 cut(s) 59, 259, 291
NarI GGCGCC 1 cut(s) 106
NciI CCSGG 2 cut(s) 688, 721
NdeII GATC 5 cut(s) 165, 189, 196, 418, 451
NlaIII CATG 8 cut(s) 42, 136, 283, 343, 425, 644, 677, 710
NlaIV GGNNCC 1 cut(s) 107
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 1 cut(s) 269
NotI GCGGCCGC 1 cut(s) 54
PasI CCCWGGG 1 cut(s) 231
PcsI WCGNNNNNNNCGW 4 cut(s) 174, 192, 198, 453
PdmI GAANNNNTTC 1 cut(s) 749
PfeI GAWTC 3 cut(s) 434, 556, 758
PflFI GACNNNGTC 1 cut(s) 88
PfoI TCCNGGA 2 cut(s) 686, 719
PkrI GCNGC 7 cut(s) 55, 58, 61, 110, 257, 264, 287
PleI GAGTC 2 cut(s) 15, 97
PluTI GGCGCC 1 cut(s) 109
PmaCI CACGTG 2 cut(s) 202, 212
PmlI CACGTG 2 cut(s) 202, 212
PpsI GAGTC 2 cut(s) 15, 97
Ppu21I YACGTR 2 cut(s) 202, 212
Psp124BI GAGCTC 1 cut(s) 32
Psp6I CCWGG 4 cut(s) 126, 230, 351, 536
PspCI CACGTG 2 cut(s) 202, 212
PspGI CCWGG 4 cut(s) 126, 230, 351, 536
PspN4I GGNNCC 1 cut(s) 107
PspPI GGNCC 2 cut(s) 84, 95
PstNI CAGNNNCTG 2 cut(s) 358, 761
PsyI GACNNNGTC 1 cut(s) 88
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
Rsr2I CGGWCCG 1 cut(s) 84
RsrII CGGWCCG 1 cut(s) 84
SacI GAGCTC 1 cut(s) 32
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 7 cut(s) 54, 57, 60, 109, 256, 263, 286
Sau3AI GATC 5 cut(s) 165, 189, 196, 418, 451
Sau96I GGNCC 2 cut(s) 84, 95
SchI GAGTC 2 cut(s) 15, 98
ScrFI CCNGG 6 cut(s) 128, 232, 353, 538, 688, 721
SduI GDGCHC 2 cut(s) 32, 82
SfaNI GCATC 1 cut(s) 727
SfoI GGCGCC 1 cut(s) 107
SinI GGWCC 1 cut(s) 84
SmlI CTYRAG 2 cut(s) 23, 302
SmoI CTYRAG 2 cut(s) 23, 302
Sse9I AATT 5 cut(s) 367, 408, 427, 566, 649
SspDI GGCGCC 1 cut(s) 105
SspI AATATT 1 cut(s) 241
SstI GAGCTC 1 cut(s) 32
StyD4I CCNGG 6 cut(s) 126, 230, 351, 536, 686, 719
StyI CCWWGG 1 cut(s) 323
TaiI ACGT 2 cut(s) 204, 214
TaqII GACCGA 1 cut(s) 101
TasI AATT 5 cut(s) 367, 408, 427, 566, 649
TauI GCSGC 6 cut(s) 56, 59, 62, 111, 258, 288
TfiI GAWTC 3 cut(s) 434, 556, 758
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 1 cut(s) 262
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 6 cut(s) 268, 447, 476, 662, 695, 728
TspGWI ACGGA 3 cut(s) 257, 326, 456
Tth111I GACNNNGTC 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 84
XapI RAATTY 2 cut(s) 566, 649
XmnI GAANNNNTTC 1 cut(s) 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.