pycom10g13160

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006566
Physical Location & Seq
Reverse (-)
16646945 .. 16649280
2336 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g13160.3

Sequence Viewer

Length: 846 bp
ATGCAAGCGGTTCGAACCGACTCGACTGTAACACTCTACTGCACCAAACTCAGAAACACTGCTTCCCAAATGCTCTCCCTAGCAAAACGACTCAAGAACTCACCGTCATGCTTCCAGACTGCGGCCGCCGCCACCCAACTGCGATCGGCCCGGACCGAGTCTGGCCCATCTCGGCGCCGCCACAAGTCACCCGCCCTGGCCATGCGGAAGCAGGAGGAGAAATCCGAGTGGTGGGTCGTTGACGGCGAAATCCACGAGATCGGCGATCACGTGCCTCCACGTGAGCGGTTCGTCATTCCCAGGGACAACATCCCCAACAAGCGCCGGAAGCAGCTCCGAGACCAGTTCATGCGCCGCACTCGCCTCGTCCTCAAGGAAACGGAACACGAGCCTTGGTGCAAAAGATACATGGAGCTTTACCAGGAGCTGAGGGAAAATTGGGAAAGGCTGTATTGGGACGAGGGTTATTCCAAAAAAATTGCTCAGGATCATGCGAATTATGAATCCGCCGAGGACGATGATCACGATTTCTCTCCGTACAGGAACAAGCGGTTTCATCCTGAGCCATTTAAGGACAATGGTTTTGCAAGTAGGCAAGGTGATACCTGGGAAAAGGTCAACCAGATTCGGGATAAATTTGAGTATGACAGAGAAAGGAGAATGAGAGAGAAAGCATTTGCTCCGATGTCTGGAGAAGCTCCTTCTAACTTCCATGATATGAATTCCCCGAGACAGCCTAACTTCCATGATATGAACTCCCGGAGACAGCCAAATGTCCATGATATGAACTCCCGGAGACAGCCATTTGACGCCCAGAAATACTTCTCGGATTCTGAAACAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

282

Amino Acids

33.7

Weight (kDa)

9.25

Isoelectric Point (pI)

68.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 174
AccBSI CCGCTC 1 cut(s) 286
AclWI GGATC 1 cut(s) 495
AcoI YGGCCR 2 cut(s) 123, 198
AcsI RAATTY 2 cut(s) 635, 721
AcvI CACGTG 2 cut(s) 271, 281
AcyI GRCGYC 2 cut(s) 175, 810
AfaI GTAC 1 cut(s) 539
AfiI CCNNNNNNNGG 4 cut(s) 121, 231, 628, 689
AjnI CCWGG 4 cut(s) 195, 299, 420, 605
AluBI AGCT 4 cut(s) 334, 415, 427, 698
AluI AGCT 4 cut(s) 334, 415, 427, 698
Alw26I GTCTC 4 cut(s) 333, 724, 757, 790
AlwI GGATC 1 cut(s) 495
AlwNI CAGNNNCTG 1 cut(s) 427
Ama87I CYCGRG 1 cut(s) 727
AoxI GGCC 4 cut(s) 123, 147, 163, 198
ApeKI GCWGC 1 cut(s) 331
ApoI RAATTY 2 cut(s) 635, 721
ArsI GACNNNNNNTTYG 2 cut(s) 566, 598
Asp700I GAANNNNTTC 1 cut(s) 821
AspLEI GCGC 3 cut(s) 177, 324, 354
AspS9I GGNCC 3 cut(s) 148, 153, 164
AsuC2I CCSGG 3 cut(s) 151, 760, 793
AsuHPI GGTGA 3 cut(s) 93, 180, 611
AsuII TTCGAA 1 cut(s) 13
AvaI CYCGRG 1 cut(s) 727
AvaII GGWCC 1 cut(s) 153
BalI TGGCCA 1 cut(s) 200
BanI GGYRCC 1 cut(s) 174
BauI CACGAG 2 cut(s) 254, 386
BbrPI CACGTG 2 cut(s) 271, 281
BbvCI CCTCAGC 1 cut(s) 428
BbvI GCAGC 1 cut(s) 343
BccI CCATC 1 cut(s) 175
BceAI ACGGC 1 cut(s) 259
BcgI CGANNNNNNTGC 3 cut(s) 27, 346, 380
BciT130I CCWGG 4 cut(s) 197, 301, 422, 607
BclI TGATCA 1 cut(s) 520
BcnI CCSGG 3 cut(s) 151, 760, 793
BcoDI GTCTC 4 cut(s) 333, 724, 757, 790
BfaI CTAG 1 cut(s) 80
BfoI RGCGCY 2 cut(s) 178, 325
BisI GCNGC 6 cut(s) 123, 126, 129, 178, 332, 355
BlsI GCNGC 6 cut(s) 124, 127, 130, 179, 333, 356
Bme1390I CCNGG 7 cut(s) 151, 197, 301, 422, 607, 760, 793
Bme18I GGWCC 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 727
BmgT120I GGNCC 3 cut(s) 148, 153, 164
BmiI GGNNCC 1 cut(s) 176
BmrFI CCNGG 7 cut(s) 151, 197, 301, 422, 607, 760, 793
BpmI CTGGAG 1 cut(s) 711
Bpu10I CCTNAGC 3 cut(s) 428, 483, 561
Bpu14I TTCGAA 1 cut(s) 13
BpuEI CTTGAG 2 cut(s) 77, 356
BpuMI CCSGG 3 cut(s) 151, 760, 793
BsaAI YACGTR 2 cut(s) 271, 281
BsaHI GRCGYC 2 cut(s) 175, 810
BsaI GGTCTC 1 cut(s) 333
BsaJI CCNNGG 6 cut(s) 195, 299, 300, 392, 510, 606
Bsc4I CCNNNNNNNGG 4 cut(s) 121, 231, 628, 689
Bse1I ACTGG 1 cut(s) 343
BseBI CCWGG 4 cut(s) 197, 301, 422, 607
BseDI CCNNGG 6 cut(s) 195, 299, 300, 392, 510, 606
BseGI GGATG 2 cut(s) 309, 556
BseLI CCNNNNNNNGG 4 cut(s) 121, 231, 628, 689
BseMII CTCAG 4 cut(s) 64, 419, 497, 552
BseNI ACTGG 1 cut(s) 343
BseRI GAGGAG 1 cut(s) 230
BseX3I CGGCCG 1 cut(s) 123
BseXI GCAGC 1 cut(s) 343
BsgI GTGCAG 1 cut(s) 25
Bsh1285I CGRYCG 2 cut(s) 126, 146
BshFI GGCC 4 cut(s) 125, 149, 165, 200
BshNI GGYRCC 1 cut(s) 174
BsiEI CGRYCG 2 cut(s) 126, 146
BsiHKCI CYCGRG 1 cut(s) 727
BsiSI CCGG 4 cut(s) 151, 325, 760, 793
BslFI GGGAC 2 cut(s) 317, 470
BslI CCNNNNNNNGG 4 cut(s) 121, 231, 628, 689
BsmAI GTCTC 4 cut(s) 333, 724, 757, 790
BsmFI GGGAC 2 cut(s) 317, 470
BsnI GGCC 4 cut(s) 125, 149, 165, 200
Bso31I GGTCTC 1 cut(s) 333
BsoBI CYCGRG 1 cut(s) 727
Bsp119I TTCGAA 1 cut(s) 13
Bsp143I GATC 5 cut(s) 143, 258, 265, 487, 520
BspANI GGCC 4 cut(s) 125, 149, 165, 200
BspCNI CTCAG 4 cut(s) 63, 420, 496, 553
BspLI GGNNCC 1 cut(s) 176
BspPI GGATC 1 cut(s) 495
BspT104I TTCGAA 1 cut(s) 13
BspT107I GGYRCC 1 cut(s) 174
BspTNI GGTCTC 1 cut(s) 333
BsrBI CCGCTC 1 cut(s) 286
BsrI ACTGG 1 cut(s) 343
BssECI CCNNGG 6 cut(s) 195, 299, 300, 392, 510, 606
BssMI GATC 5 cut(s) 143, 258, 265, 487, 520
BssNI GRCGYC 2 cut(s) 175, 810
BssSI CACGAG 2 cut(s) 254, 386
BssT1I CCWWGG 1 cut(s) 392
Bst2BI CACGAG 2 cut(s) 254, 386
Bst2UI CCWGG 4 cut(s) 197, 301, 422, 607
Bst4CI ACNGT 2 cut(s) 28, 105
BstACI GRCGYC 2 cut(s) 175, 810
BstBAI YACGTR 2 cut(s) 271, 281
BstBI TTCGAA 1 cut(s) 13
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 4 cut(s) 50, 428, 483, 561
BstF5I GGATG 2 cut(s) 309, 556
BstH2I RGCGCY 2 cut(s) 178, 325
BstHHI GCGC 3 cut(s) 177, 324, 354
BstKTI GATC 5 cut(s) 146, 261, 268, 490, 523
BstMAI GTCTC 4 cut(s) 333, 724, 757, 790
BstMBI GATC 5 cut(s) 143, 258, 265, 487, 520
BstMCI CGRYCG 2 cut(s) 126, 146
BstMWI GCNNNNNNNGC 3 cut(s) 128, 328, 360
BstNI CCWGG 4 cut(s) 197, 301, 422, 607
BstSCI CCNGG 7 cut(s) 149, 195, 299, 420, 605, 758, 791
BstV1I GCAGC 1 cut(s) 343
BstZI CGGCCG 1 cut(s) 123
BsuRI GGCC 4 cut(s) 125, 149, 165, 200
BtsCI GGATG 2 cut(s) 309, 556
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 1 cut(s) 57
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 1 cut(s) 427
CciNI GCGGCCGC 1 cut(s) 123
CfoI GCGC 3 cut(s) 177, 324, 354
Cfr13I GGNCC 3 cut(s) 148, 153, 164
CpoI CGGWCCG 1 cut(s) 153
CseI GACGC 1 cut(s) 818
Csp6I GTAC 1 cut(s) 538
CspI CGGWCCG 1 cut(s) 153
CviAII CATG 8 cut(s) 108, 202, 349, 409, 491, 713, 746, 779
CviQI GTAC 1 cut(s) 538
DdeI CTNAG 4 cut(s) 50, 428, 483, 561
DinI GGCGCC 1 cut(s) 176
DpnI GATC 5 cut(s) 145, 260, 267, 489, 522
DpnII GATC 5 cut(s) 143, 258, 265, 487, 520
EaeI YGGCCR 2 cut(s) 123, 198
EagI CGGCCG 1 cut(s) 123
EciI GGCGGA 1 cut(s) 496
EclXI CGGCCG 1 cut(s) 123
Eco130I CCWWGG 1 cut(s) 392
Eco31I GGTCTC 1 cut(s) 333
Eco47I GGWCC 1 cut(s) 153
Eco52I CGGCCG 1 cut(s) 123
Eco72I CACGTG 2 cut(s) 271, 281
Eco88I CYCGRG 1 cut(s) 727
EcoRI GAATTC 1 cut(s) 721
EcoRII CCWGG 4 cut(s) 195, 299, 420, 605
EcoT14I CCWWGG 1 cut(s) 392
EgeI GGCGCC 1 cut(s) 176
EheI GGCGCC 1 cut(s) 176
ErhI CCWWGG 1 cut(s) 392
FaeI CATG 8 cut(s) 111, 205, 352, 412, 494, 716, 749, 782
FaqI GGGAC 2 cut(s) 317, 470
FatI CATG 8 cut(s) 107, 201, 348, 408, 490, 712, 745, 778
FauI CCCGC 1 cut(s) 199
FbaI TGATCA 1 cut(s) 520
Fnu4HI GCNGC 6 cut(s) 123, 126, 129, 178, 332, 355
FokI GGATG 2 cut(s) 296, 543
Fsp4HI GCNGC 6 cut(s) 123, 126, 129, 178, 332, 355
FspBI CTAG 1 cut(s) 80
GlaI GCGC 3 cut(s) 176, 323, 353
GluI GCNGC 6 cut(s) 123, 126, 129, 178, 332, 355
GsuI CTGGAG 1 cut(s) 711
HaeII RGCGCY 2 cut(s) 178, 325
HaeIII GGCC 4 cut(s) 125, 149, 165, 200
HapII CCGG 4 cut(s) 151, 325, 760, 793
HgaI GACGC 1 cut(s) 818
HhaI GCGC 3 cut(s) 177, 324, 354
Hin1I GRCGYC 2 cut(s) 175, 810
Hin1II CATG 8 cut(s) 111, 205, 352, 412, 494, 716, 749, 782
Hin6I GCGC 3 cut(s) 175, 322, 352
HinP1I GCGC 3 cut(s) 175, 322, 352
HincII GTYRAC 2 cut(s) 241, 619
HindII GTYRAC 2 cut(s) 241, 619
HinfI GANTC 6 cut(s) 20, 90, 158, 503, 625, 830
HpaII CCGG 4 cut(s) 151, 325, 760, 793
HphI GGTGA 3 cut(s) 93, 180, 611
Hpy166II GTNNAC 2 cut(s) 241, 619
Hpy188I TCNGA 6 cut(s) 53, 226, 338, 684, 829, 835
Hpy188III TCNNGA 7 cut(s) 94, 115, 485, 524, 560, 629, 690
Hpy8I GTNNAC 2 cut(s) 241, 619
HpyAV CCTTC 1 cut(s) 711
HpyCH4III ACNGT 2 cut(s) 28, 105
HpyCH4IV ACGT 2 cut(s) 270, 280
HpyCH4V TGCA 4 cut(s) 4, 42, 399, 587
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 328, 360
HpyF3I CTNAG 4 cut(s) 50, 428, 483, 561
HpySE526I ACGT 2 cut(s) 270, 280
Hsp92I GRCGYC 2 cut(s) 175, 810
Hsp92II CATG 8 cut(s) 111, 205, 352, 412, 494, 716, 749, 782
HspAI GCGC 3 cut(s) 175, 322, 352
KasI GGCGCC 1 cut(s) 174
Ksp22I TGATCA 1 cut(s) 520
Kzo9I GATC 5 cut(s) 143, 258, 265, 487, 520
LmnI GCTCC 5 cut(s) 339, 412, 424, 685, 703
Lsp1109I GCAGC 1 cut(s) 343
MaeI CTAG 1 cut(s) 80
MaeII ACGT 2 cut(s) 270, 280
MaeIII GTNAC 2 cut(s) 28, 186
MalI GATC 5 cut(s) 145, 260, 267, 489, 522
MbiI CCGCTC 1 cut(s) 286
MboI GATC 5 cut(s) 143, 258, 265, 487, 520
MlsI TGGCCA 1 cut(s) 200
MluCI AATT 5 cut(s) 436, 477, 496, 635, 721
MluNI TGGCCA 1 cut(s) 200
Mly113I GGCGCC 1 cut(s) 175
MlyI GAGTC 3 cut(s) 14, 84, 167
MnlI CCTC 7 cut(s) 208, 285, 374, 380, 423, 454, 505
Mox20I TGGCCA 1 cut(s) 200
MroXI GAANNNNTTC 1 cut(s) 821
MscI TGGCCA 1 cut(s) 200
MseI TTAA 1 cut(s) 570
MslI CAYNNNNRTG 1 cut(s) 106
Msp20I TGGCCA 1 cut(s) 200
MspI CCGG 4 cut(s) 151, 325, 760, 793
MspR9I CCNGG 7 cut(s) 151, 197, 301, 422, 607, 760, 793
MvaI CCWGG 4 cut(s) 197, 301, 422, 607
MwoI GCNNNNNNNGC 3 cut(s) 128, 328, 360
NarI GGCGCC 1 cut(s) 175
NciI CCSGG 3 cut(s) 151, 760, 793
NdeII GATC 5 cut(s) 143, 258, 265, 487, 520
NlaIII CATG 8 cut(s) 111, 205, 352, 412, 494, 716, 749, 782
NlaIV GGNNCC 1 cut(s) 176
NmeAIII GCCGAG 2 cut(s) 151, 535
NmuCI GTSAC 1 cut(s) 186
NotI GCGGCCGC 1 cut(s) 123
NspV TTCGAA 1 cut(s) 13
PasI CCCWGGG 1 cut(s) 300
PcsI WCGNNNNNNNCGW 4 cut(s) 243, 261, 267, 522
PdmI GAANNNNTTC 1 cut(s) 821
PfeI GAWTC 3 cut(s) 503, 625, 830
PflFI GACNNNGTC 1 cut(s) 157
PfoI TCCNGGA 2 cut(s) 758, 791
PkrI GCNGC 6 cut(s) 124, 127, 130, 179, 333, 356
Ple19I CGATCG 1 cut(s) 146
PleI GAGTC 3 cut(s) 14, 84, 166
PluTI GGCGCC 1 cut(s) 178
PmaCI CACGTG 2 cut(s) 271, 281
PmlI CACGTG 2 cut(s) 271, 281
PpsI GAGTC 3 cut(s) 14, 84, 166
Ppu21I YACGTR 2 cut(s) 271, 281
Psp6I CCWGG 4 cut(s) 195, 299, 420, 605
PspCI CACGTG 2 cut(s) 271, 281
PspGI CCWGG 4 cut(s) 195, 299, 420, 605
PspN4I GGNNCC 1 cut(s) 176
PspPI GGNCC 3 cut(s) 148, 153, 164
PstNI CAGNNNCTG 1 cut(s) 427
PsyI GACNNNGTC 1 cut(s) 157
PvuI CGATCG 1 cut(s) 146
RsaI GTAC 1 cut(s) 539
RsaNI GTAC 1 cut(s) 538
RseI CAYNNNNRTG 1 cut(s) 106
Rsr2I CGGWCCG 1 cut(s) 153
RsrII CGGWCCG 1 cut(s) 153
SaqAI TTAA 1 cut(s) 570
SatI GCNGC 6 cut(s) 123, 126, 129, 178, 332, 355
Sau3AI GATC 5 cut(s) 143, 258, 265, 487, 520
Sau96I GGNCC 3 cut(s) 148, 153, 164
SchI GAGTC 3 cut(s) 14, 84, 167
ScrFI CCNGG 7 cut(s) 151, 197, 301, 422, 607, 760, 793
SetI ASST 9 cut(s) 273, 283, 336, 417, 429, 601, 608, 618, 700
SfoI GGCGCC 1 cut(s) 176
SfuI TTCGAA 1 cut(s) 13
SinI GGWCC 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 106
SmlI CTYRAG 2 cut(s) 92, 371
SmoI CTYRAG 2 cut(s) 92, 371
Sse9I AATT 5 cut(s) 436, 477, 496, 635, 721
SspDI GGCGCC 1 cut(s) 174
SspMI CTAG 1 cut(s) 80
StyD4I CCNGG 7 cut(s) 149, 195, 299, 420, 605, 758, 791
StyI CCWWGG 1 cut(s) 392
TaaI ACNGT 2 cut(s) 28, 105
TaiI ACGT 2 cut(s) 273, 283
TaqI TCGA 2 cut(s) 13, 23
TaqII GACCGA 1 cut(s) 170
TasI AATT 5 cut(s) 436, 477, 496, 635, 721
TauI GCSGC 5 cut(s) 125, 128, 131, 180, 357
TfiI GAWTC 3 cut(s) 503, 625, 830
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 1 cut(s) 186
TseI GCWGC 1 cut(s) 331
Tsp45I GTSAC 1 cut(s) 186
TspDTI ATGAA 6 cut(s) 337, 516, 545, 734, 767, 800
TspGWI ACGGA 2 cut(s) 395, 525
TspRI CASTG 1 cut(s) 64
Tth111I GACNNNGTC 1 cut(s) 157
VpaK11BI GGWCC 1 cut(s) 153
XapI RAATTY 2 cut(s) 635, 721
XmnI GAANNNNTTC 1 cut(s) 821
XspI CTAG 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.