MD10G1179300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010790
Physical Location & Seq
Forward (+)
27298444 .. 27298866
423 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1179300.v1.1.491

Sequence Viewer

Length: 423 bp
ATGAACACCAAAACGATGCGTTTGCCACCTCGTCGGATTTCGACGACCAATGCGTCAAATAATACCAACAACAACAACAAGCGCAAGGAGAAGGAGAGTGGCTTCGACGCGCTCCAGAAGACCAAGACGCTCAAGCCGGCCCCACCGCCCCAAGCCAAGCCGTCCCAGCCGGCCCCACCGCCCGGTCAGCTCCTGGCTGGCTATCTGGCGCACGAGTTCCTCACCAAGGGCACCCTCCTCGGGCACCCGTGGGACCCGTCCCGAGCCGAGCCGGCTCCTTCGGGCGTCAGCAAGATCGAAGCCGAGCCGAAGTTGGAGAACTTGGAGAGATACGTGGAAGTCGCTGATTTGCTGAAGACGGAAGGGGCCCACTTGGCTGGCATTGTGAACCCAACTCAGCTCGCTCGGATTTTACAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

15.27

Weight (kDa)

9.74

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010790)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04000 AT5G44060
fragaria_vesca FvH4_2g20950
malus_domestica MD05G1192900.v1.1 MD10G1179300.v1.1
prunus_persica Prupe.8G219100_v2.0.a1
pyrus_communis pycom05g17980 pycom10g15550
rosa_chinensis RchiOBHm_Chr6g0287331
rosa_laevigata RLG00000012498
rosa_multiflora Rmu_co8349819.1_g000001 Rmu_sc0004701.1_g000001
rosa_roxburghii Rroxscaffold_7G00181530
rosa_rugosa Rorug06G0187100
rosa_samantha Rh6AG298500 Rh6BG304100 Rh6CG305600 Rh6CG312600 Rh6DG295900
rosa_wichuraiana Rw6G025800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 52
AccB1I GGYRCC 2 cut(s) 230, 243
AccII CGCG 1 cut(s) 110
AciI CCGC 2 cut(s) 146, 179
AcuI CTGAAG 1 cut(s) 374
AcyI GRCGYC 1 cut(s) 285
AfiI CCNNNNNNNGG 3 cut(s) 182, 226, 240
AjnI CCWGG 1 cut(s) 192
AluBI AGCT 2 cut(s) 190, 400
AluI AGCT 2 cut(s) 190, 400
AlwNI CAGNNNCTG 1 cut(s) 193
Ama87I CYCGRG 2 cut(s) 239, 261
AoxI GGCC 3 cut(s) 138, 171, 366
ApaI GGGCCC 1 cut(s) 370
AspLEI GCGC 3 cut(s) 84, 112, 211
AspS9I GGNCC 5 cut(s) 139, 172, 253, 366, 367
AsuC2I CCSGG 1 cut(s) 183
AsuHPI GGTGA 1 cut(s) 214
AvaI CYCGRG 2 cut(s) 239, 261
AvaII GGWCC 1 cut(s) 253
BaeGI GKGCMC 3 cut(s) 233, 246, 370
BanI GGYRCC 2 cut(s) 230, 243
BanII GRGCYC 1 cut(s) 370
BauI CACGAG 1 cut(s) 212
BbsI GAAGAC 2 cut(s) 125, 362
BceAI ACGGC 1 cut(s) 145
BcgI CGANNNNNNTGC 4 cut(s) 4, 38, 220, 254
BciT130I CCWGG 1 cut(s) 194
BcnI CCSGG 1 cut(s) 183
BglI GCCNNNNNGGC 2 cut(s) 272, 374
Bme1390I CCNGG 2 cut(s) 183, 194
Bme18I GGWCC 1 cut(s) 253
BmeT110I CYCGRG 2 cut(s) 239, 261
BmgT120I GGNCC 5 cut(s) 139, 172, 253, 366, 367
BmiI GGNNCC 9 cut(s) 141, 174, 232, 245, 254, 255, 276, 367, 368
BmrFI CCNGG 2 cut(s) 183, 194
BmsI GCATC 1 cut(s) 6
BpiI GAAGAC 2 cut(s) 125, 362
BpmI CTGGAG 1 cut(s) 98
BpuEI CTTGAG 1 cut(s) 116
BpuMI CCSGG 1 cut(s) 183
BsaAI YACGTR 1 cut(s) 334
BsaHI GRCGYC 1 cut(s) 285
BsaJI CCNNGG 3 cut(s) 225, 238, 248
Bsc4I CCNNNNNNNGG 3 cut(s) 182, 226, 240
Bse118I RCCGGY 3 cut(s) 136, 169, 271
BseBI CCWGG 1 cut(s) 194
BseDI CCNNGG 3 cut(s) 225, 238, 248
BseLI CCNNNNNNNGG 3 cut(s) 182, 226, 240
BseMII CTCAG 1 cut(s) 410
BseRI GAGGAG 1 cut(s) 227
BseSI GKGCMC 3 cut(s) 233, 246, 370
BseYI CCCAGC 1 cut(s) 165
Bsh1236I CGCG 1 cut(s) 110
BshFI GGCC 3 cut(s) 140, 173, 368
BshNI GGYRCC 2 cut(s) 230, 243
BsiHKCI CYCGRG 2 cut(s) 239, 261
BsiSI CCGG 4 cut(s) 137, 170, 183, 272
BslFI GGGAC 3 cut(s) 148, 244, 266
BslI CCNNNNNNNGG 3 cut(s) 182, 226, 240
BsmFI GGGAC 3 cut(s) 148, 244, 266
BsnI GGCC 3 cut(s) 140, 173, 368
BsoBI CYCGRG 2 cut(s) 239, 261
Bsp120I GGGCCC 1 cut(s) 366
Bsp1286I GDGCHC 3 cut(s) 233, 246, 370
Bsp143I GATC 1 cut(s) 294
BspACI CCGC 2 cut(s) 146, 179
BspANI GGCC 3 cut(s) 140, 173, 368
BspCNI CTCAG 1 cut(s) 409
BspFNI CGCG 1 cut(s) 110
BspLI GGNNCC 9 cut(s) 141, 174, 232, 245, 254, 255, 276, 367, 368
BspT107I GGYRCC 2 cut(s) 230, 243
BsrFI RCCGGY 3 cut(s) 136, 169, 271
BssAI RCCGGY 3 cut(s) 136, 169, 271
BssECI CCNNGG 3 cut(s) 225, 238, 248
BssMI GATC 1 cut(s) 294
BssNI GRCGYC 1 cut(s) 285
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 1 cut(s) 225
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 1 cut(s) 417
BstACI GRCGYC 1 cut(s) 285
BstBAI YACGTR 1 cut(s) 334
BstC8I GCNNGC 6 cut(s) 138, 171, 199, 273, 379, 402
BstDEI CTNAG 1 cut(s) 396
BstDSI CCRYGG 1 cut(s) 248
BstENI CCTNNNNNAGG 1 cut(s) 224
BstFNI CGCG 1 cut(s) 110
BstHHI GCGC 3 cut(s) 84, 112, 211
BstKTI GATC 1 cut(s) 297
BstMBI GATC 1 cut(s) 294
BstMWI GCNNNNNNNGC 4 cut(s) 166, 187, 272, 374
BstNI CCWGG 1 cut(s) 194
BstSCI CCNGG 2 cut(s) 181, 192
BstSLI GKGCMC 3 cut(s) 233, 246, 370
BstUI CGCG 1 cut(s) 110
BstV2I GAAGAC 2 cut(s) 125, 362
BstXI CCANNNNNNTGG 1 cut(s) 377
BsuRI GGCC 3 cut(s) 140, 173, 368
BtgI CCRYGG 1 cut(s) 248
Cac8I GCNNGC 6 cut(s) 138, 171, 199, 273, 379, 402
CaiI CAGNNNCTG 1 cut(s) 193
CfoI GCGC 3 cut(s) 84, 112, 211
Cfr10I RCCGGY 3 cut(s) 136, 169, 271
Cfr13I GGNCC 5 cut(s) 139, 172, 253, 366, 367
CseI GACGC 4 cut(s) 42, 116, 136, 274
DdeI CTNAG 1 cut(s) 396
DpnI GATC 1 cut(s) 296
DpnII GATC 1 cut(s) 294
DrdI GACNNNNNNGTC 1 cut(s) 52
DseDI GACNNNNNNGTC 1 cut(s) 52
Eco130I CCWWGG 1 cut(s) 225
Eco24I GRGCYC 1 cut(s) 370
Eco47I GGWCC 1 cut(s) 253
Eco57I CTGAAG 1 cut(s) 374
Eco88I CYCGRG 2 cut(s) 239, 261
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoO109I RGGNCCY 2 cut(s) 253, 366
EcoRII CCWGG 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 225
EcoT38I GRGCYC 1 cut(s) 370
ErhI CCWWGG 1 cut(s) 225
FaqI GGGAC 3 cut(s) 148, 244, 266
FriOI GRGCYC 1 cut(s) 370
GlaI GCGC 3 cut(s) 83, 111, 210
GsaI CCCAGC 1 cut(s) 169
GsuI CTGGAG 1 cut(s) 98
HaeIII GGCC 3 cut(s) 140, 173, 368
HapII CCGG 4 cut(s) 137, 170, 183, 272
HgaI GACGC 4 cut(s) 42, 116, 136, 274
HhaI GCGC 3 cut(s) 84, 112, 211
Hin1I GRCGYC 1 cut(s) 285
Hin6I GCGC 3 cut(s) 82, 110, 209
HinP1I GCGC 3 cut(s) 82, 110, 209
HpaII CCGG 4 cut(s) 137, 170, 183, 272
HphI GGTGA 1 cut(s) 214
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 2 cut(s) 36, 408
Hpy188III TCNNGA 2 cut(s) 115, 261
Hpy8I GTNNAC 1 cut(s) 388
Hpy99I CGWCG 3 cut(s) 36, 46, 110
HpyAV CCTTC 3 cut(s) 85, 288, 356
HpyCH4III ACNGT 1 cut(s) 417
HpyCH4IV ACGT 1 cut(s) 333
HpyF10VI GCNNNNNNNGC 4 cut(s) 166, 187, 272, 374
HpyF3I CTNAG 1 cut(s) 396
HpySE526I ACGT 1 cut(s) 333
Hsp92I GRCGYC 1 cut(s) 285
HspAI GCGC 3 cut(s) 82, 110, 209
KflI GGGWCCC 1 cut(s) 253
KroI GCCGGC 3 cut(s) 136, 169, 271
KroNI GCCGGC 3 cut(s) 138, 171, 273
Kzo9I GATC 1 cut(s) 294
LmnI GCTCC 3 cut(s) 117, 195, 280
LweI GCATC 1 cut(s) 6
MaeII ACGT 1 cut(s) 333
MalI GATC 1 cut(s) 296
MboI GATC 1 cut(s) 294
MboII GAAGA 2 cut(s) 130, 367
MhlI GDGCHC 3 cut(s) 233, 246, 370
MmeI TCCRAC 2 cut(s) 14, 294
MnlI CCTC 4 cut(s) 39, 230, 245, 248
MroNI GCCGGC 3 cut(s) 136, 169, 271
MspI CCGG 4 cut(s) 137, 170, 183, 272
MspR9I CCNGG 2 cut(s) 183, 194
MvaI CCWGG 1 cut(s) 194
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 4 cut(s) 166, 187, 272, 374
NaeI GCCGGC 3 cut(s) 138, 171, 273
NciI CCSGG 1 cut(s) 183
NdeII GATC 1 cut(s) 294
NgoMIV GCCGGC 3 cut(s) 136, 169, 271
NlaIV GGNNCC 9 cut(s) 141, 174, 232, 245, 254, 255, 276, 367, 368
NmeAIII GCCGAG 2 cut(s) 292, 328
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PdiI GCCGGC 3 cut(s) 138, 171, 273
Ppu21I YACGTR 1 cut(s) 334
PpuMI RGGWCCY 1 cut(s) 253
Psp5II RGGWCCY 1 cut(s) 253
Psp6I CCWGG 1 cut(s) 192
PspFI CCCAGC 1 cut(s) 165
PspGI CCWGG 1 cut(s) 192
PspN4I GGNNCC 9 cut(s) 141, 174, 232, 245, 254, 255, 276, 367, 368
PspOMI GGGCCC 1 cut(s) 366
PspPI GGNCC 5 cut(s) 139, 172, 253, 366, 367
PspPPI RGGWCCY 1 cut(s) 253
PstNI CAGNNNCTG 1 cut(s) 193
Sau3AI GATC 1 cut(s) 294
Sau96I GGNCC 5 cut(s) 139, 172, 253, 366, 367
ScrFI CCNGG 2 cut(s) 183, 194
SduI GDGCHC 3 cut(s) 233, 246, 370
SetI ASST 4 cut(s) 31, 192, 336, 402
SfaNI GCATC 1 cut(s) 6
SinI GGWCC 1 cut(s) 253
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
SsiI CCGC 2 cut(s) 146, 179
StyD4I CCNGG 2 cut(s) 181, 192
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 1 cut(s) 417
TaiI ACGT 1 cut(s) 336
TaqI TCGA 3 cut(s) 41, 105, 297
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 374
VpaK11BI GGWCC 1 cut(s) 253
XagI CCTNNNNNAGG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.