pycom05g17980

chloroplast organization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010790
Physical Location & Seq
Forward (+)
21060299 .. 21060781
483 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g17980.1

Sequence Viewer

Length: 483 bp
ATGAACTGCTGCTGCACACGTGGCATGCACACAGAGAGCGATCATATGAATACCAAAACGATGCGTTTGCCACCTCGTCGGATTTCGACGACCAATGCGTCAAATAATGCCAACAGCAACAACAAGCGCAAGGAGAAGGAGGGTGACTTCGACGCGCTCCAGCAGACCAAGACGCTCAAGCTGGCCCCACCGCCCCAAGCCAAGCCGTCCGAGCCAGCCCCGCCGCCCGGTCAGCCCGGTCAGCTCCTGGCTGGTTATCTGGCGCACGAGTTCCTCACCAAGGGCACCCTCCTCGGGCAGCCGTGGGACCCCTCCCGAGCCGAGCCGGCTCTACCGGGAGTCAGCAGCAGCAAGATCGAAGCCGAGCCGAAGTTCGAGAGCTTGGAGAGATACGTGGAAGTCGCTGATTTGCTGAAAACGGAGGGGGCCCACTTGGCTGGCGTTGTGAATCCAACTCAGCTCGCTGGGTTTTTACAGTTGTGA

Protein Analysis

161

Amino Acids

17.37

Weight (kDa)

7.74

Isoelectric Point (pI)

52.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010790)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04000 AT5G44060
fragaria_vesca FvH4_2g20950
malus_domestica MD05G1192900.v1.1 MD10G1179300.v1.1
prunus_persica Prupe.8G219100_v2.0.a1
pyrus_communis pycom05g17980 pycom10g15550
rosa_chinensis RchiOBHm_Chr6g0287331
rosa_laevigata RLG00000012498
rosa_multiflora Rmu_co8349819.1_g000001 Rmu_sc0004701.1_g000001
rosa_roxburghii Rroxscaffold_7G00181530
rosa_rugosa Rorug06G0187100
rosa_samantha Rh6AG298500 Rh6BG304100 Rh6CG305600 Rh6CG312600 Rh6DG295900
rosa_wichuraiana Rw6G025800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 97
AccB1I GGYRCC 1 cut(s) 284
AccII CGCG 1 cut(s) 155
AciI CCGC 3 cut(s) 191, 221, 224
AcvI CACGTG 1 cut(s) 20
AfiI CCNNNNNNNGG 3 cut(s) 227, 280, 294
AflIII ACRYGT 1 cut(s) 17
AjnI CCWGG 1 cut(s) 246
AluBI AGCT 4 cut(s) 181, 244, 381, 460
AluI AGCT 4 cut(s) 181, 244, 381, 460
AlwNI CAGNNNCTG 1 cut(s) 247
Ama87I CYCGRG 2 cut(s) 293, 315
AoxI GGCC 2 cut(s) 183, 426
ApaI GGGCCC 1 cut(s) 430
ApeKI GCWGC 5 cut(s) 9, 12, 298, 345, 348
AspLEI GCGC 3 cut(s) 129, 157, 265
AspS9I GGNCC 4 cut(s) 184, 307, 426, 427
AsuC2I CCSGG 3 cut(s) 228, 237, 336
AsuHPI GGTGA 2 cut(s) 155, 268
AvaI CYCGRG 2 cut(s) 293, 315
AvaII GGWCC 1 cut(s) 307
BaeGI GKGCMC 2 cut(s) 287, 430
BanI GGYRCC 1 cut(s) 284
BanII GRGCYC 1 cut(s) 430
BauI CACGAG 1 cut(s) 266
BbrPI CACGTG 1 cut(s) 20
BbvI GCAGC 3 cut(s) 310, 357, 360
BceAI ACGGC 2 cut(s) 190, 286
BcgI CGANNNNNNTGC 6 cut(s) 49, 83, 274, 308, 337, 371
BciT130I CCWGG 1 cut(s) 248
BcnI CCSGG 3 cut(s) 228, 237, 336
BglI GCCNNNNNGGC 2 cut(s) 326, 434
BisI GCNGC 6 cut(s) 10, 13, 224, 299, 346, 349
BlsI GCNGC 6 cut(s) 11, 14, 225, 300, 347, 350
Bme1390I CCNGG 4 cut(s) 228, 237, 248, 336
Bme18I GGWCC 1 cut(s) 307
BmeT110I CYCGRG 2 cut(s) 293, 315
BmgT120I GGNCC 4 cut(s) 184, 307, 426, 427
BmiI GGNNCC 6 cut(s) 186, 286, 308, 309, 427, 428
BmrFI CCNGG 4 cut(s) 228, 237, 248, 336
BmsI GCATC 1 cut(s) 51
BpmI CTGGAG 1 cut(s) 143
BpuEI CTTGAG 1 cut(s) 161
BpuMI CCSGG 3 cut(s) 228, 237, 336
BsaAI YACGTR 2 cut(s) 20, 394
BsaJI CCNNGG 3 cut(s) 279, 292, 302
Bsc4I CCNNNNNNNGG 3 cut(s) 227, 280, 294
Bse118I RCCGGY 1 cut(s) 325
BseBI CCWGG 1 cut(s) 248
BseDI CCNNGG 3 cut(s) 279, 292, 302
BseLI CCNNNNNNNGG 3 cut(s) 227, 280, 294
BseMII CTCAG 1 cut(s) 470
BseRI GAGGAG 1 cut(s) 281
BseSI GKGCMC 2 cut(s) 287, 430
BseXI GCAGC 3 cut(s) 310, 357, 360
BseYI CCCAGC 1 cut(s) 464
Bsh1236I CGCG 1 cut(s) 155
BshFI GGCC 2 cut(s) 185, 428
BshNI GGYRCC 1 cut(s) 284
BsiHKCI CYCGRG 2 cut(s) 293, 315
BsiSI CCGG 4 cut(s) 228, 237, 326, 335
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 3 cut(s) 227, 280, 294
BsmFI GGGAC 1 cut(s) 320
BsnI GGCC 2 cut(s) 185, 428
BsoBI CYCGRG 2 cut(s) 293, 315
Bsp120I GGGCCC 1 cut(s) 426
Bsp1286I GDGCHC 2 cut(s) 287, 430
Bsp143I GATC 2 cut(s) 40, 354
BspACI CCGC 3 cut(s) 191, 221, 224
BspANI GGCC 2 cut(s) 185, 428
BspCNI CTCAG 1 cut(s) 469
BspFNI CGCG 1 cut(s) 155
BspLI GGNNCC 6 cut(s) 186, 286, 308, 309, 427, 428
BspT107I GGYRCC 1 cut(s) 284
BsrFI RCCGGY 1 cut(s) 325
BssAI RCCGGY 1 cut(s) 325
BssECI CCNNGG 3 cut(s) 279, 292, 302
BssMI GATC 2 cut(s) 40, 354
BssSI CACGAG 1 cut(s) 266
BssT1I CCWWGG 1 cut(s) 279
Bst2BI CACGAG 1 cut(s) 266
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 1 cut(s) 477
BstBAI YACGTR 2 cut(s) 20, 394
BstC8I GCNNGC 6 cut(s) 26, 183, 216, 327, 439, 462
BstDEI CTNAG 1 cut(s) 456
BstDSI CCRYGG 1 cut(s) 302
BstENI CCTNNNNNAGG 1 cut(s) 278
BstFNI CGCG 1 cut(s) 155
BstHHI GCGC 3 cut(s) 129, 157, 265
BstKTI GATC 2 cut(s) 43, 357
BstMBI GATC 2 cut(s) 40, 354
BstMWI GCNNNNNNNGC 7 cut(s) 21, 211, 220, 232, 241, 326, 434
BstNI CCWGG 1 cut(s) 248
BstNSI RCATGY 1 cut(s) 28
BstSCI CCNGG 4 cut(s) 226, 235, 246, 334
BstSLI GKGCMC 2 cut(s) 287, 430
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 3 cut(s) 310, 357, 360
BstXI CCANNNNNNTGG 1 cut(s) 437
BsuRI GGCC 2 cut(s) 185, 428
BtgI CCRYGG 1 cut(s) 302
Cac8I GCNNGC 6 cut(s) 26, 183, 216, 327, 439, 462
CaiI CAGNNNCTG 1 cut(s) 247
CfoI GCGC 3 cut(s) 129, 157, 265
Cfr10I RCCGGY 1 cut(s) 325
Cfr13I GGNCC 4 cut(s) 184, 307, 426, 427
CseI GACGC 3 cut(s) 87, 161, 181
CviAII CATG 1 cut(s) 25
DdeI CTNAG 1 cut(s) 456
DpnI GATC 2 cut(s) 42, 356
DpnII GATC 2 cut(s) 40, 354
DrdI GACNNNNNNGTC 1 cut(s) 97
DseDI GACNNNNNNGTC 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 279
Eco24I GRGCYC 1 cut(s) 430
Eco47I GGWCC 1 cut(s) 307
Eco72I CACGTG 1 cut(s) 20
Eco88I CYCGRG 2 cut(s) 293, 315
EcoNI CCTNNNNNAGG 1 cut(s) 278
EcoO109I RGGNCCY 2 cut(s) 307, 426
EcoRII CCWGG 1 cut(s) 246
EcoT14I CCWWGG 1 cut(s) 279
EcoT38I GRGCYC 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 279
FaeI CATG 1 cut(s) 28
FaiI YATR 3 cut(s) 26, 45, 47
FaqI GGGAC 1 cut(s) 320
FatI CATG 1 cut(s) 24
FauI CCCGC 1 cut(s) 228
FauNDI CATATG 1 cut(s) 45
Fnu4HI GCNGC 6 cut(s) 10, 13, 224, 299, 346, 349
FriOI GRGCYC 1 cut(s) 430
Fsp4HI GCNGC 6 cut(s) 10, 13, 224, 299, 346, 349
GlaI GCGC 3 cut(s) 128, 156, 264
GluI GCNGC 6 cut(s) 10, 13, 224, 299, 346, 349
GsaI CCCAGC 1 cut(s) 468
GsuI CTGGAG 1 cut(s) 143
HaeIII GGCC 2 cut(s) 185, 428
HapII CCGG 4 cut(s) 228, 237, 326, 335
HgaI GACGC 3 cut(s) 87, 161, 181
HhaI GCGC 3 cut(s) 129, 157, 265
Hin1II CATG 1 cut(s) 28
Hin6I GCGC 3 cut(s) 127, 155, 263
HinP1I GCGC 3 cut(s) 127, 155, 263
HinfI GANTC 2 cut(s) 339, 448
HpaII CCGG 4 cut(s) 228, 237, 326, 335
HphI GGTGA 2 cut(s) 155, 268
Hpy188I TCNGA 2 cut(s) 81, 211
Hpy188III TCNNGA 2 cut(s) 315, 376
Hpy99I CGWCG 3 cut(s) 81, 91, 155
HpyAV CCTTC 1 cut(s) 130
HpyCH4III ACNGT 1 cut(s) 477
HpyCH4IV ACGT 2 cut(s) 19, 393
HpyCH4V TGCA 2 cut(s) 15, 28
HpyF10VI GCNNNNNNNGC 7 cut(s) 21, 211, 220, 232, 241, 326, 434
HpyF3I CTNAG 1 cut(s) 456
HpySE526I ACGT 2 cut(s) 19, 393
Hsp92II CATG 1 cut(s) 28
HspAI GCGC 3 cut(s) 127, 155, 263
KflI GGGWCCC 1 cut(s) 307
KroI GCCGGC 1 cut(s) 325
KroNI GCCGGC 1 cut(s) 327
Kzo9I GATC 2 cut(s) 40, 354
LmnI GCTCC 2 cut(s) 162, 249
Lsp1109I GCAGC 3 cut(s) 310, 357, 360
LweI GCATC 1 cut(s) 51
MaeII ACGT 2 cut(s) 19, 393
MaeIII GTNAC 1 cut(s) 143
MalI GATC 2 cut(s) 42, 356
MboI GATC 2 cut(s) 40, 354
MhlI GDGCHC 2 cut(s) 287, 430
MlyI GAGTC 1 cut(s) 348
MmeI TCCRAC 2 cut(s) 59, 476
MnlI CCTC 7 cut(s) 84, 133, 284, 299, 302, 322, 415
MroNI GCCGGC 1 cut(s) 325
MspI CCGG 4 cut(s) 228, 237, 326, 335
MspR9I CCNGG 4 cut(s) 228, 237, 248, 336
MvaI CCWGG 1 cut(s) 248
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 7 cut(s) 21, 211, 220, 232, 241, 326, 434
NaeI GCCGGC 1 cut(s) 327
NciI CCSGG 3 cut(s) 228, 237, 336
NdeI CATATG 1 cut(s) 45
NdeII GATC 2 cut(s) 40, 354
NgoMIV GCCGGC 1 cut(s) 325
NlaIII CATG 1 cut(s) 28
NlaIV GGNNCC 6 cut(s) 186, 286, 308, 309, 427, 428
NmeAIII GCCGAG 2 cut(s) 346, 388
NmuCI GTSAC 1 cut(s) 143
NspI RCATGY 1 cut(s) 28
PaeI GCATGC 1 cut(s) 28
PcsI WCGNNNNNNNCGW 1 cut(s) 95
PdiI GCCGGC 1 cut(s) 327
PfeI GAWTC 1 cut(s) 448
PkrI GCNGC 6 cut(s) 11, 14, 225, 300, 347, 350
PleI GAGTC 1 cut(s) 347
PmaCI CACGTG 1 cut(s) 20
PmlI CACGTG 1 cut(s) 20
PpsI GAGTC 1 cut(s) 347
Ppu21I YACGTR 2 cut(s) 20, 394
PpuMI RGGWCCY 1 cut(s) 307
Psp5II RGGWCCY 1 cut(s) 307
Psp6I CCWGG 1 cut(s) 246
PspCI CACGTG 1 cut(s) 20
PspFI CCCAGC 1 cut(s) 464
PspGI CCWGG 1 cut(s) 246
PspN4I GGNNCC 6 cut(s) 186, 286, 308, 309, 427, 428
PspOMI GGGCCC 1 cut(s) 426
PspPI GGNCC 4 cut(s) 184, 307, 426, 427
PspPPI RGGWCCY 1 cut(s) 307
PstNI CAGNNNCTG 1 cut(s) 247
SatI GCNGC 6 cut(s) 10, 13, 224, 299, 346, 349
Sau3AI GATC 2 cut(s) 40, 354
Sau96I GGNCC 4 cut(s) 184, 307, 426, 427
SchI GAGTC 1 cut(s) 348
ScrFI CCNGG 4 cut(s) 228, 237, 248, 336
SduI GDGCHC 2 cut(s) 287, 430
SetI ASST 7 cut(s) 22, 76, 183, 246, 383, 396, 462
SfaNI GCATC 1 cut(s) 51
SinI GGWCC 1 cut(s) 307
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
SphI GCATGC 1 cut(s) 28
SsiI CCGC 3 cut(s) 191, 221, 224
StyD4I CCNGG 4 cut(s) 226, 235, 246, 334
StyI CCWWGG 1 cut(s) 279
TaaI ACNGT 1 cut(s) 477
TaiI ACGT 2 cut(s) 22, 396
TaqI TCGA 4 cut(s) 86, 150, 357, 375
TauI GCSGC 1 cut(s) 226
TfiI GAWTC 1 cut(s) 448
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 5 cut(s) 9, 12, 298, 345, 348
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 2 cut(s) 17, 62
TspGWI ACGGA 1 cut(s) 434
VpaK11BI GGWCC 1 cut(s) 307
XagI CCTNNNNNAGG 1 cut(s) 278
XceI RCATGY 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.