Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003818

Physical Location & Seq

Genomic Coordinates Forward (+)

33257033 .. 33260765

Gene Structure 3733 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1236000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1569 bp

ATGGAGGGTCAGGCAATTTCTGGTGATGAGGTTTCTGTTAATTTAGTTAGAGAGGAAGACGAAGAAGAGTTCCGGAGCTGTTGTGAAGACGAAGATGAAGTTTGGAAGGAGACCGAAGAACCAGTGAAAGGGGAGACAAAAGATGAGTCAAAATATGATCTCGACGAATTTTCTGTGAAAATGTTCTTCAAAGGCATGTCCATAGCTGGCTATGGGGACCCCGGTTGTGGACTTTCTGGAATCGGAGTTGTCATGGAAAGATCAACCAATGTTCCTGCAATTCAGGTGCAGAAAAGGCTTGATTTTTATGTGGAGGAGCTTGTGGCTGACTATTTAGCCCTGATGGATGGTTTAGTGGAGGCTGTGCAGAATAAAGTCCGTAGGGTGTATGCATTTACAGATTCTGAACTGCTTTATGATCAGGTTTCACATGAGGAGAAACTTGAAGTTCCAATCCTAGTGGCACTGAGGCAAAGGATTTTAGAGCATGCCAGTAATCTTGAAGCTTTTGTTCTGAAACTTGCCCCTGATGTAGATCTTGAGCGGCCATCAAAATTAGCCCAAGTGGCTATTGGAGTTGTCTCTTTTCCGGCTAAGGGTGTTGAATCTCTTGAAAACTGTTCCATCTGTTGCGACGATAAACCATCCTTGATGATGATCACCATGAAATGTTCTCATAAGTTCTGTTCACATTGCATGAGAACATACGTTGATGGGAAGGTACAGTCCTCTCAAGTCCCCATCAGATGTCCTCAACTGAGATGCAAGTATTACATCTCCACTGCTGAGTGCAAATCTTTTCTTCCACTCACTTCTTACGAGTCATTGGAGAAATCCTTTGCAGAAGCAAGTATTCTCCACTCAGATAGAATGTACTGCCCATTTCCAAATTGTTCCGTCCTGCTTGATCCCCTTGAATGCTTGTCAGCTAGGGCAAGTTCATCAAGTCAGTCGGATAACAGCTGCATTGAATGTCCAGTTTGTCAGAGGTTCATCTGTGCGGATTGTGGGGTTCCTTGGCATACTTCTATGAGCTGCGAGGAGTTCCAGAATCTCCCATTGGAAGAGAGAGATGCTGCAGACATTACCCTACATCGCTTGGCACAAAGTAACAGCTGGAGGCGTTGCCAGCAGTGTCGTAGGATGATTGAACTCTCTCAAGGTTGCTACCACATGACGTGCTGGTGTGGGCATGAGTTCTGTTATTCTTGTGGTGCGGAATATCGGGATAGCCAACAGACTTGTCAATGTGCCTTTTGGGACGAAGACAACAACACACAAGACTTGGTCACTCAGTCTATGCAAGAATCCGAGCAATGGGCGTGGGAAACATTCAATTCACTCCCCATGATAATGGATGCATACTCGGACCAAGAGAGATCGCAGCTGGCGCTGATCCAGAGATTCCTAGCTGGGGGGTTCAGTTTAAGCGACCATCCCCCTTATCAATCCCCACCTCGGTGTACAGATTCTTATGCAGACGCCATGAAAGATCTCCATCAGCTTCCTTGGCTCGAGAGGTTTGTTTCCGTTATAAGTGATAACTACTACGAAGAATATATCCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

IBR RVT_3 zf-C3HC4

Protein Analysis

523

Amino Acids

59.51

Weight (kDa)

4.6

Isoelectric Point (pI)

57.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RVT_3	PF13456	77 - 174	2.2e-07	Reverse transcriptase-like
IBR	PF01485	280 - 346	1.2e-07	IBR domain, a half RING-finger domain
IBR	PF01485	367 - 415	2.1e-06	IBR domain, a half RING-finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003818)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19670 AT4G19670 AT4G19670 AT4G19670 AT4G19670 AT4G19670 AT4G19670
fragaria_vesca	FvH4_3g12010 FvH4_3g12010 FvH4_3g12010 FvH4_3g12010
malus_domestica	MD05G1255900.v1.1 MD10G1236000.v1.1 MD10G1236100.v1.1
prunus_persica	Prupe.4G108300_v2.0.a1 Prupe.4G108300_v2.0.a1 Prupe.4G108300_v2.0.a1 Prupe.4G108300_v2.0.a1
pyrus_communis	pycom05g23470 pycom10g19720 pycom10g19730
rosa_chinensis	RchiOBHm_Chr5g0019691
rosa_laevigata	RLG00000032461
rosa_multiflora	Rmu_co8286553.1_g000001 Rmu_sc0012330.1_g000002
rosa_roxburghii	Rroxscaffold_1G00058420
rosa_rugosa	Rorug05G0052500 Rorug05G0052500 Rorug05G0052600
rosa_samantha	Rh5AG143300 Rh5BG141900 Rh5CG153800 Rh5DG142500
rosa_wichuraiana	Rw5G012700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1535
AccBSI	CCGCTC	1 cut(s)	544
AccIII	TCCGGA	1 cut(s)	72
AciI	CCGC	3 cut(s)	544, 1001, 1217
AclWI	GGATC	2 cut(s)	902, 1390
AcoI	YGGCCR	1 cut(s)	545
AcsI	RAATTY	1 cut(s)	167
AcyI	GRCGYC	1 cut(s)	1482
AfaI	GTAC	3 cut(s)	723, 875, 1465
AfiI	CCNNNNNNNGG	6 cut(s)	128, 227, 596, 1317, 1414, 1458
AgsI	TTSAA	9 cut(s)	190, 446, 503, 605, 614, 917, 971, 1151, 1336
AjiI	CACGTC	1 cut(s)	1179
AjuI	GAANNNNNNNTTGG	2 cut(s)	1043, 1075
AleI	CACNNNNGTG	1 cut(s)	1459
Alw26I	GTCTC	3 cut(s)	104, 128, 586
AlwI	GGATC	2 cut(s)	902, 1390
AlwNI	CAGNNNCTG	1 cut(s)	404
Ama87I	CYCGRG	1 cut(s)	1514
Aor13HI	TCCGGA	1 cut(s)	72
AoxI	GGCC	1 cut(s)	545
ApeKI	GCWGC	4 cut(s)	963, 1035, 1076, 1384
ApoI	RAATTY	1 cut(s)	167
ArsI	GACNNNNNNTTYG	2 cut(s)	131, 163
Asp700I	GAANNNNTTC	1 cut(s)	182
AspLEI	GCGC	1 cut(s)	1393
AspS9I	GGNCC	2 cut(s)	217, 1369
AsuC2I	CCSGG	1 cut(s)	222
AsuHPI	GGTGA	2 cut(s)	35, 652
AvaI	CYCGRG	1 cut(s)	1514
AvaII	GGWCC	2 cut(s)	217, 1369
BbsI	GAAGAC	3 cut(s)	63, 93, 1272
BbvI	GCAGC	4 cut(s)	950, 1022, 1063, 1396
BccI	CCATC	9 cut(s)	337, 341, 556, 632, 652, 707, 749, 1443, 1506
BclI	TGATCA	2 cut(s)	418, 657
BcnI	CCSGG	1 cut(s)	222
BcoDI	GTCTC	3 cut(s)	104, 128, 586
BfaI	CTAG	3 cut(s)	458, 930, 1409
BfmI	CTRYAG	1 cut(s)	1077
BfoI	RGCGCY	1 cut(s)	1394
BglI	GCCNNNNNGGC	1 cut(s)	566
BglII	AGATCT	2 cut(s)	535, 1492
BisI	GCNGC	5 cut(s)	545, 964, 1036, 1077, 1385
BlsI	GCNGC	5 cut(s)	546, 965, 1037, 1078, 1386
Bme1390I	CCNGG	1 cut(s)	222
Bme18I	GGWCC	2 cut(s)	217, 1369
BmeT110I	CYCGRG	1 cut(s)	1514
BmgBI	CACGTC	1 cut(s)	1179
BmgT120I	GGNCC	2 cut(s)	217, 1369
BmiI	GGNNCC	3 cut(s)	218, 219, 1014
BmrFI	CCNGG	1 cut(s)	222
BmsI	GCATC	3 cut(s)	752, 1063, 1348
BpiI	GAAGAC	3 cut(s)	63, 93, 1272
BpmI	CTGGAG	1 cut(s)	1138
Bpu10I	CCTNAGC	1 cut(s)	594
BpuEI	CTTGAG	3 cut(s)	560, 717, 1143
BpuMI	CCSGG	1 cut(s)	222
BsaBI	GATNNNNATC	3 cut(s)	534, 656, 1497
BsaHI	GRCGYC	1 cut(s)	1482
BsaI	GGTCTC	1 cut(s)	104
BsaJI	CCNNGG	4 cut(s)	220, 1016, 1457, 1508
BsaWI	WCCGGW	1 cut(s)	72
BsaXI	ACNNNNNCTCC	2 cut(s)	67, 97
Bsc4I	CCNNNNNNNGG	6 cut(s)	128, 227, 596, 1317, 1414, 1458
Bse1I	ACTGG	4 cut(s)	122, 492, 977, 1564
Bse3DI	GCAATG	2 cut(s)	691, 1322
Bse8I	GATNNNNATC	3 cut(s)	534, 656, 1497
BseAI	TCCGGA	1 cut(s)	72
BseDI	CCNNGG	4 cut(s)	220, 1016, 1457, 1508
BseGI	GGATG	5 cut(s)	352, 644, 1149, 1363, 1435
BseJI	GATNNNNATC	3 cut(s)	534, 656, 1497
BseLI	CCNNNNNNNGG	6 cut(s)	128, 227, 596, 1317, 1414, 1458
BseMI	GCAATG	2 cut(s)	691, 1322
BseMII	CTCAG	5 cut(s)	458, 749, 777, 876, 1307
BseNI	ACTGG	4 cut(s)	122, 492, 977, 1564
BseRI	GAGGAG	3 cut(s)	329, 449, 1055
BseXI	GCAGC	4 cut(s)	950, 1022, 1063, 1396
BseYI	CCCAGC	1 cut(s)	1412
BsgI	GTGCAG	2 cut(s)	308, 386
BshFI	GGCC	1 cut(s)	547
BsiHKCI	CYCGRG	1 cut(s)	1514
BsiSI	CCGG	3 cut(s)	73, 222, 590
BslFI	GGGAC	3 cut(s)	230, 722, 1274
BslI	CCNNNNNNNGG	6 cut(s)	128, 227, 596, 1317, 1414, 1458
BsmAI	GTCTC	3 cut(s)	104, 128, 586
BsmFI	GGGAC	3 cut(s)	230, 722, 1274
BsmI	GAATGC	1 cut(s)	923
BsnI	GGCC	1 cut(s)	547
Bso31I	GGTCTC	1 cut(s)	104
BsoBI	CYCGRG	1 cut(s)	1514
Bsp13I	TCCGGA	1 cut(s)	72
Bsp1407I	TGTACA	1 cut(s)	1463
Bsp143I	GATC	9 cut(s)	157, 260, 418, 535, 657, 907, 1379, 1395, 1492
BspACI	CCGC	3 cut(s)	544, 1001, 1217
BspANI	GGCC	1 cut(s)	547
BspCNI	CTCAG	5 cut(s)	459, 750, 778, 875, 1306
BspEI	TCCGGA	1 cut(s)	72
BspLI	GGNNCC	3 cut(s)	218, 219, 1014
BspMAI	CTGCAG	1 cut(s)	1081
BspPI	GGATC	2 cut(s)	902, 1390
BspTNI	GGTCTC	1 cut(s)	104
BsrBI	CCGCTC	1 cut(s)	544
BsrDI	GCAATG	2 cut(s)	691, 1322
BsrGI	TGTACA	1 cut(s)	1463
BsrI	ACTGG	4 cut(s)	122, 492, 977, 1564
BssECI	CCNNGG	4 cut(s)	220, 1016, 1457, 1508
BssMI	GATC	9 cut(s)	157, 260, 418, 535, 657, 907, 1379, 1395, 1492
BssNI	GRCGYC	1 cut(s)	1482
BssT1I	CCWWGG	2 cut(s)	1016, 1508
Bst4CI	ACNGT	2 cut(s)	620, 726
Bst6I	CTCTTC	2 cut(s)	60, 1059
BstACI	GRCGYC	1 cut(s)	1482
BstAUI	TGTACA	1 cut(s)	1463
BstC8I	GCNNGC	4 cut(s)	208, 489, 1130, 1389
BstDEI	CTNAG	6 cut(s)	467, 594, 758, 786, 862, 1293
BstF5I	GGATG	5 cut(s)	352, 644, 1149, 1363, 1435
BstH2I	RGCGCY	1 cut(s)	1394
BstHHI	GCGC	1 cut(s)	1393
BstKTI	GATC	9 cut(s)	160, 263, 421, 538, 660, 910, 1382, 1398, 1495
BstMAI	GTCTC	3 cut(s)	104, 128, 586
BstMBI	GATC	9 cut(s)	157, 260, 418, 535, 657, 907, 1379, 1395, 1492
BstMWI	GCNNNNNNNGC	5 cut(s)	295, 566, 1129, 1390, 1510
BstNSI	RCATGY	2 cut(s)	199, 491
BstSCI	CCNGG	1 cut(s)	220
BstSFI	CTRYAG	1 cut(s)	1077
BstV1I	GCAGC	4 cut(s)	950, 1022, 1063, 1396
BstV2I	GAAGAC	3 cut(s)	63, 93, 1272
BstX2I	RGATCY	2 cut(s)	535, 1492
BstXI	CCANNNNNNTGG	1 cut(s)	1354
BstYI	RGATCY	2 cut(s)	535, 1492
BsuRI	GGCC	1 cut(s)	547
BtgZI	GCGATG	1 cut(s)	1079
BtrI	CACGTC	1 cut(s)	1179
BtsCI	GGATG	5 cut(s)	352, 644, 1149, 1363, 1435
BtsI	GCAGTG	2 cut(s)	780, 1139
BtsIMutI	CAGTG	4 cut(s)	129, 464, 780, 1139
Cac8I	GCNNGC	4 cut(s)	208, 489, 1130, 1389
CaiI	CAGNNNCTG	1 cut(s)	404
CfoI	GCGC	1 cut(s)	1393
Cfr13I	GGNCC	2 cut(s)	217, 1369
CseI	GACGC	1 cut(s)	1490
Csp6I	GTAC	3 cut(s)	722, 874, 1464
CspCI	CAANNNNNGTGG	4 cut(s)	441, 476, 1304, 1339
CviQI	GTAC	3 cut(s)	722, 874, 1464
DdeI	CTNAG	6 cut(s)	467, 594, 758, 786, 862, 1293
DpnI	GATC	9 cut(s)	159, 262, 420, 537, 659, 909, 1381, 1397, 1494
DpnII	GATC	9 cut(s)	157, 260, 418, 535, 657, 907, 1379, 1395, 1492
EaeI	YGGCCR	1 cut(s)	545
Eam1104I	CTCTTC	2 cut(s)	60, 1059
EarI	CTCTTC	2 cut(s)	60, 1059
Eco130I	CCWWGG	2 cut(s)	1016, 1508
Eco31I	GGTCTC	1 cut(s)	104
Eco47I	GGWCC	2 cut(s)	217, 1369
Eco88I	CYCGRG	1 cut(s)	1514
EcoO109I	RGGNCCY	1 cut(s)	217
EcoT14I	CCWWGG	2 cut(s)	1016, 1508
EcoT22I	ATGCAT	2 cut(s)	394, 1363
ErhI	CCWWGG	2 cut(s)	1016, 1508
FaqI	GGGAC	3 cut(s)	230, 722, 1274
FbaI	TGATCA	2 cut(s)	418, 657
Fnu4HI	GCNGC	5 cut(s)	545, 964, 1036, 1077, 1385
FokI	GGATG	5 cut(s)	359, 631, 1156, 1370, 1422
Fsp4HI	GCNGC	5 cut(s)	545, 964, 1036, 1077, 1385
FspBI	CTAG	3 cut(s)	458, 930, 1409
GlaI	GCGC	1 cut(s)	1392
GluI	GCNGC	5 cut(s)	545, 964, 1036, 1077, 1385
GsaI	CCCAGC	1 cut(s)	1416
GsuI	CTGGAG	1 cut(s)	1138
HaeII	RGCGCY	1 cut(s)	1394
HaeIII	GGCC	1 cut(s)	547
HapII	CCGG	3 cut(s)	73, 222, 590
HgaI	GACGC	1 cut(s)	1490
HhaI	GCGC	1 cut(s)	1393
Hin1I	GRCGYC	1 cut(s)	1482
Hin6I	GCGC	1 cut(s)	1391
HinP1I	GCGC	1 cut(s)	1391
HindIII	AAGCTT	1 cut(s)	504
HinfI	GANTC	9 cut(s)	146, 240, 401, 605, 821, 1051, 1307, 1404, 1469
HpaII	CCGG	3 cut(s)	73, 222, 590
HphI	GGTGA	2 cut(s)	35, 652
Hpy166II	GTNNAC	3 cut(s)	230, 689, 1464
Hpy188I	TCNGA	9 cut(s)	245, 406, 516, 746, 865, 955, 987, 1312, 1369
Hpy8I	GTNNAC	3 cut(s)	230, 689, 1464
Hpy99I	CGWCG	2 cut(s)	167, 638
HpyAV	CCTTC	2 cut(s)	100, 712
HpyCH4III	ACNGT	2 cut(s)	620, 726
HpyCH4IV	ACGT	2 cut(s)	708, 1178
HpyF10VI	GCNNNNNNNGC	5 cut(s)	295, 566, 1129, 1390, 1510
HpyF3I	CTNAG	6 cut(s)	467, 594, 758, 786, 862, 1293
HpySE526I	ACGT	2 cut(s)	708, 1178
Hsp92I	GRCGYC	1 cut(s)	1482
HspAI	GCGC	1 cut(s)	1391
KflI	GGGWCCC	1 cut(s)	217
Kpn2I	TCCGGA	1 cut(s)	72
Ksp22I	TGATCA	2 cut(s)	418, 657
Kzo9I	GATC	9 cut(s)	157, 260, 418, 535, 657, 907, 1379, 1395, 1492
LmnI	GCTCC	2 cut(s)	75, 316
Lsp1109I	GCAGC	4 cut(s)	950, 1022, 1063, 1396
LweI	GCATC	3 cut(s)	752, 1063, 1348
MaeI	CTAG	3 cut(s)	458, 930, 1409
MaeII	ACGT	2 cut(s)	708, 1178
MaeIII	GTNAC	2 cut(s)	1109, 1288
MalI	GATC	9 cut(s)	159, 262, 420, 537, 659, 909, 1381, 1397, 1494
MbiI	CCGCTC	1 cut(s)	544
MboI	GATC	9 cut(s)	157, 260, 418, 535, 657, 907, 1379, 1395, 1492
MflI	RGATCY	2 cut(s)	535, 1492
MluCI	AATT	7 cut(s)	15, 40, 167, 279, 554, 889, 1336
MlyI	GAGTC	2 cut(s)	155, 830
MmeI	TCCRAC	1 cut(s)	933
Mph1103I	ATGCAT	2 cut(s)	394, 1363
MroI	TCCGGA	1 cut(s)	72
MroXI	GAANNNNTTC	1 cut(s)	182
MseI	TTAA	2 cut(s)	39, 1427
MslI	CAYNNNNRTG	2 cut(s)	1352, 1459
MspA1I	CMGCKG	3 cut(s)	963, 1116, 1387
MspI	CCGG	3 cut(s)	73, 222, 590
MspR9I	CCNGG	1 cut(s)	222
Mva1269I	GAATGC	1 cut(s)	923
MwoI	GCNNNNNNNGC	5 cut(s)	295, 566, 1129, 1390, 1510
NciI	CCSGG	1 cut(s)	222
NdeII	GATC	9 cut(s)	157, 260, 418, 535, 657, 907, 1379, 1395, 1492
NlaIV	GGNNCC	3 cut(s)	218, 219, 1014
NmuCI	GTSAC	1 cut(s)	1288
NsiI	ATGCAT	2 cut(s)	394, 1363
NspI	RCATGY	2 cut(s)	199, 491
OliI	CACNNNNGTG	1 cut(s)	1459
PaeI	GCATGC	1 cut(s)	491
PaeR7I	CTCGAG	1 cut(s)	1514
PctI	GAATGC	1 cut(s)	923
PdmI	GAANNNNTTC	1 cut(s)	182
PfeI	GAWTC	7 cut(s)	240, 401, 605, 1051, 1307, 1404, 1469
PflFI	GACNNNGTC	1 cut(s)	1286
PkrI	GCNGC	5 cut(s)	546, 965, 1037, 1078, 1386
PleI	GAGTC	2 cut(s)	154, 829
PpsI	GAGTC	2 cut(s)	154, 829
PpuMI	RGGWCCY	1 cut(s)	217
PsiI	TTATAA	1 cut(s)	1535
Psp5II	RGGWCCY	1 cut(s)	217
PspFI	CCCAGC	1 cut(s)	1412
PspN4I	GGNNCC	3 cut(s)	218, 219, 1014
PspPI	GGNCC	2 cut(s)	217, 1369
PspPPI	RGGWCCY	1 cut(s)	217
PstI	CTGCAG	1 cut(s)	1081
PstNI	CAGNNNCTG	1 cut(s)	404
PsuI	RGATCY	2 cut(s)	535, 1492
PsyI	GACNNNGTC	1 cut(s)	1286
PvuII	CAGCTG	3 cut(s)	963, 1116, 1387
RsaI	GTAC	3 cut(s)	723, 875, 1465
RsaNI	GTAC	3 cut(s)	722, 874, 1464
RseI	CAYNNNNRTG	2 cut(s)	1352, 1459
SaqAI	TTAA	2 cut(s)	39, 1427
SatI	GCNGC	5 cut(s)	545, 964, 1036, 1077, 1385
Sau3AI	GATC	9 cut(s)	157, 260, 418, 535, 657, 907, 1379, 1395, 1492
Sau96I	GGNCC	2 cut(s)	217, 1369
SchI	GAGTC	2 cut(s)	155, 830
ScrFI	CCNGG	1 cut(s)	222
SfaNI	GCATC	3 cut(s)	752, 1063, 1348
SfcI	CTRYAG	1 cut(s)	1077
Sfr274I	CTCGAG	1 cut(s)	1514
SinI	GGWCC	2 cut(s)	217, 1369
SlaI	CTCGAG	1 cut(s)	1514
SmiMI	CAYNNNNRTG	2 cut(s)	1352, 1459
SmlI	CTYRAG	4 cut(s)	539, 732, 1158, 1514
SmoI	CTYRAG	4 cut(s)	539, 732, 1158, 1514
SphI	GCATGC	1 cut(s)	491
Sse9I	AATT	7 cut(s)	15, 40, 167, 279, 554, 889, 1336
SsiI	CCGC	3 cut(s)	544, 1001, 1217
SspMI	CTAG	3 cut(s)	458, 930, 1409
StyD4I	CCNGG	1 cut(s)	220
StyI	CCWWGG	2 cut(s)	1016, 1508
TaaI	ACNGT	2 cut(s)	620, 726
TaiI	ACGT	2 cut(s)	711, 1181
TaqI	TCGA	2 cut(s)	162, 1515
TaqII	GACCGA	1 cut(s)	128
TasI	AATT	7 cut(s)	15, 40, 167, 279, 554, 889, 1336
TatI	WGTACW	2 cut(s)	873, 1463
TauI	GCSGC	1 cut(s)	547
TfiI	GAWTC	7 cut(s)	240, 401, 605, 1051, 1307, 1404, 1469
Tru1I	TTAA	2 cut(s)	39, 1427
Tru9I	TTAA	2 cut(s)	39, 1427
TscAI	CASTG	4 cut(s)	129, 471, 787, 1139
TseFI	GTSAC	1 cut(s)	1288
TseI	GCWGC	4 cut(s)	963, 1035, 1076, 1384
Tsp45I	GTSAC	1 cut(s)	1288
TspDTI	ATGAA	5 cut(s)	111, 680, 930, 982, 1502
TspGWI	ACGGA	3 cut(s)	368, 886, 1519
TspRI	CASTG	4 cut(s)	129, 471, 787, 1139
Tth111I	GACNNNGTC	1 cut(s)	1286
VpaK11BI	GGWCC	2 cut(s)	217, 1369
XapI	RAATTY	1 cut(s)	167
XceI	RCATGY	2 cut(s)	199, 491
XcmI	CCANNNNNNNNNTGG	1 cut(s)	569
XhoI	CTCGAG	1 cut(s)	1514
XmnI	GAANNNNTTC	1 cut(s)	182
XspI	CTAG	3 cut(s)	458, 930, 1409
Zsp2I	ATGCAT	2 cut(s)	394, 1363

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.