Rorug05G0052500

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000834
Physical Location & Seq
Forward (+)
4575101 .. 4577232
2132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0052500.1

Sequence Viewer

Length: 546 bp
ATGGCTTCAAGGCTGGTCTTGGCTGTCACTTTTGTGCTTGATCTTATTGCCTTTGCTCTTGCTGTGGCTGCTGAGCAGAGGAGGTCAAACTCTAAGATTCAGAAAGATGGAAATTCCAGCTACTGTGTGTATGACTCCGACATTGCCACCGGCTTAGGTGTTGGGGCATTTCTGTTCCTGTTGACAAGTCAGGCTCTGTTAATGATTGTAAGTCGATGCTTGTGCTGTGGAAAGGCTTTGAGACCGAGTGGTTCTAGGTCATGGGCAATCATGCTATTCATTACCAGCTGGGTATTTTTCTGTATTGCTGAGGCGTGCTTACTTGCGGGTTCAGTGAGAAATGCCTACCACACCAAGTACAGGACCAAGTTACCTACTGAAAACCTTTCATGTGAGACATTGAGGAAGGGGATCTTCGGGGCTGGCGCTGCCTTTGTGGTCTTCACAGGCATAGTCTCTGAGTTCTACTATGCTAGTTACTCCAAGGCTAATGATGCAGAGGCTCACTACGCCAGAGACACTGGAGTGAGGATGCAGAACCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

181

Amino Acids

19.76

Weight (kDa)

9.02

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 56 - 150 2.6e-30 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 326
AclWI GGATC 1 cut(s) 419
AcsI RAATTY 1 cut(s) 112
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 1 cut(s) 360
AgsI TTSAA 1 cut(s) 9
AleI CACNNNNGTG 2 cut(s) 32, 524
AluBI AGCT 2 cut(s) 120, 288
AluI AGCT 2 cut(s) 120, 288
Alw26I GTCTC 4 cut(s) 235, 389, 460, 510
AlwI GGATC 1 cut(s) 419
AlwNI CAGNNNCTG 2 cut(s) 123, 196
ApeKI GCWGC 2 cut(s) 68, 428
ApoI RAATTY 1 cut(s) 112
AspLEI GCGC 1 cut(s) 428
AspS9I GGNCC 1 cut(s) 363
AvaII GGWCC 1 cut(s) 363
BbsI GAAGAC 1 cut(s) 433
BbvCI CCTCAGC 1 cut(s) 309
BbvI GCAGC 2 cut(s) 55, 415
BccI CCATC 1 cut(s) 101
BcgI CGANNNNNNTGC 2 cut(s) 204, 238
BcoDI GTCTC 4 cut(s) 235, 389, 460, 510
BfaI CTAG 2 cut(s) 255, 474
BfoI RGCGCY 1 cut(s) 429
BisI GCNGC 2 cut(s) 69, 429
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 2 cut(s) 70, 430
Bme18I GGWCC 1 cut(s) 363
BmgT120I GGNCC 1 cut(s) 363
BmsI GCATC 3 cut(s) 206, 484, 522
BpiI GAAGAC 1 cut(s) 433
BpmI CTGGAG 1 cut(s) 543
Bpu10I CCTNAGC 2 cut(s) 154, 309
Bpu1102I GCTNAGC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 235
BsaJI CCNNGG 1 cut(s) 483
Bsc4I CCNNNNNNNGG 1 cut(s) 360
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 526
Bse3DI GCAATG 1 cut(s) 141
BseDI CCNNGG 1 cut(s) 483
BseGI GGATG 1 cut(s) 537
BseLI CCNNNNNNNGG 1 cut(s) 360
BseMI GCAATG 1 cut(s) 141
BseMII CTCAG 3 cut(s) 63, 300, 450
BseNI ACTGG 1 cut(s) 526
BseRI GAGGAG 1 cut(s) 94
BseXI GCAGC 2 cut(s) 55, 415
BseYI CCCAGC 1 cut(s) 288
BsiSI CCGG 1 cut(s) 150
BslI CCNNNNNNNGG 1 cut(s) 360
BsmAI GTCTC 4 cut(s) 235, 389, 460, 510
Bso31I GGTCTC 1 cut(s) 235
Bsp143I GATC 2 cut(s) 40, 411
Bsp1720I GCTNAGC 1 cut(s) 72
BspACI CCGC 1 cut(s) 326
BspCNI CTCAG 3 cut(s) 64, 301, 451
BspPI GGATC 1 cut(s) 419
BspTNI GGTCTC 1 cut(s) 235
BsrDI GCAATG 1 cut(s) 141
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 1 cut(s) 526
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 1 cut(s) 483
BssMI GATC 2 cut(s) 40, 411
BssT1I CCWWGG 1 cut(s) 483
Bst4CI ACNGT 1 cut(s) 125
BstC8I GCNNGC 2 cut(s) 316, 424
BstDEI CTNAG 5 cut(s) 72, 93, 154, 309, 459
BstF5I GGATG 1 cut(s) 537
BstH2I RGCGCY 1 cut(s) 429
BstHHI GCGC 1 cut(s) 428
BstKTI GATC 2 cut(s) 43, 414
BstMAI GTCTC 4 cut(s) 235, 389, 460, 510
BstMBI GATC 2 cut(s) 40, 411
BstMWI GCNNNNNNNGC 4 cut(s) 68, 428, 494, 509
BstV1I GCAGC 2 cut(s) 55, 415
BstV2I GAAGAC 1 cut(s) 433
BstX2I RGATCY 1 cut(s) 411
BstYI RGATCY 1 cut(s) 411
BtsCI GGATG 1 cut(s) 537
BtsIMutI CAGTG 2 cut(s) 339, 519
Cac8I GCNNGC 2 cut(s) 316, 424
CaiI CAGNNNCTG 2 cut(s) 123, 196
CfoI GCGC 1 cut(s) 428
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 363
Csp6I GTAC 1 cut(s) 358
CviAII CATG 3 cut(s) 261, 271, 390
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 5 cut(s) 72, 93, 154, 309, 459
DpnI GATC 2 cut(s) 42, 413
DpnII GATC 2 cut(s) 40, 411
Eco130I CCWWGG 1 cut(s) 483
Eco31I GGTCTC 1 cut(s) 235
Eco47I GGWCC 1 cut(s) 363
EcoT14I CCWWGG 1 cut(s) 483
ErhI CCWWGG 1 cut(s) 483
FaeI CATG 3 cut(s) 264, 274, 393
FaiI YATR 6 cut(s) 132, 262, 272, 391, 452, 471
FalI AAGNNNNNCTT 2 cut(s) 398, 430
FatI CATG 3 cut(s) 260, 270, 389
FauI CCCGC 1 cut(s) 319
Fnu4HI GCNGC 2 cut(s) 69, 429
Fsp4HI GCNGC 2 cut(s) 69, 429
FspBI CTAG 2 cut(s) 255, 474
GlaI GCGC 1 cut(s) 427
GluI GCNGC 2 cut(s) 69, 429
GsaI CCCAGC 1 cut(s) 292
GsuI CTGGAG 1 cut(s) 543
HaeII RGCGCY 1 cut(s) 429
HapII CCGG 1 cut(s) 150
HhaI GCGC 1 cut(s) 428
Hin1II CATG 3 cut(s) 264, 274, 393
Hin6I GCGC 1 cut(s) 426
HinP1I GCGC 1 cut(s) 426
HincII GTYRAC 1 cut(s) 183
HindII GTYRAC 1 cut(s) 183
HinfI GANTC 2 cut(s) 97, 134
HpaII CCGG 1 cut(s) 150
Hpy166II GTNNAC 1 cut(s) 183
Hpy188I TCNGA 3 cut(s) 102, 139, 460
Hpy8I GTNNAC 1 cut(s) 183
HpyAV CCTTC 1 cut(s) 400
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4V TGCA 2 cut(s) 497, 535
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 428, 494, 509
HpyF3I CTNAG 5 cut(s) 72, 93, 154, 309, 459
Hsp92II CATG 3 cut(s) 264, 274, 393
HspAI GCGC 1 cut(s) 426
Kzo9I GATC 2 cut(s) 40, 411
Lsp1109I GCAGC 2 cut(s) 55, 415
LweI GCATC 3 cut(s) 206, 484, 522
MaeI CTAG 2 cut(s) 255, 474
MaeIII GTNAC 3 cut(s) 25, 369, 476
MalI GATC 2 cut(s) 42, 413
MboI GATC 2 cut(s) 40, 411
MboII GAAGA 2 cut(s) 406, 433
MflI RGATCY 1 cut(s) 411
MluCI AATT 1 cut(s) 112
MlyI GAGTC 1 cut(s) 128
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 6 cut(s) 72, 75, 304, 396, 493, 522
MseI TTAA 1 cut(s) 200
MslI CAYNNNNRTG 2 cut(s) 32, 524
MspA1I CMGCKG 1 cut(s) 288
MspI CCGG 1 cut(s) 150
MwoI GCNNNNNNNGC 4 cut(s) 68, 428, 494, 509
NdeII GATC 2 cut(s) 40, 411
NlaIII CATG 3 cut(s) 264, 274, 393
NmuCI GTSAC 1 cut(s) 25
OliI CACNNNNGTG 2 cut(s) 32, 524
PfeI GAWTC 1 cut(s) 97
PkrI GCNGC 2 cut(s) 70, 430
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
PspFI CCCAGC 1 cut(s) 288
PspPI GGNCC 1 cut(s) 363
PstNI CAGNNNCTG 2 cut(s) 123, 196
PsuI RGATCY 1 cut(s) 411
PvuII CAGCTG 1 cut(s) 288
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
RseI CAYNNNNRTG 2 cut(s) 32, 524
SaqAI TTAA 1 cut(s) 200
SatI GCNGC 2 cut(s) 69, 429
Sau3AI GATC 2 cut(s) 40, 411
Sau96I GGNCC 1 cut(s) 363
SchI GAGTC 1 cut(s) 128
SetI ASST 8 cut(s) 86, 122, 160, 260, 290, 376, 387, 543
SfaNI GCATC 3 cut(s) 206, 484, 522
SinI GGWCC 1 cut(s) 363
SmiMI CAYNNNNRTG 2 cut(s) 32, 524
Sse9I AATT 1 cut(s) 112
SsiI CCGC 1 cut(s) 326
SspMI CTAG 2 cut(s) 255, 474
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 1 cut(s) 125
TaqI TCGA 1 cut(s) 214
TaqII GACCGA 1 cut(s) 259
TasI AATT 1 cut(s) 112
TatI WGTACW 1 cut(s) 357
TfiI GAWTC 1 cut(s) 97
Tru1I TTAA 1 cut(s) 200
Tru9I TTAA 1 cut(s) 200
TscAI CASTG 2 cut(s) 339, 526
TseFI GTSAC 1 cut(s) 25
TseI GCWGC 2 cut(s) 68, 428
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 2 cut(s) 268, 378
TspRI CASTG 2 cut(s) 339, 526
VpaK11BI GGWCC 1 cut(s) 363
XapI RAATTY 1 cut(s) 112
XspI CTAG 2 cut(s) 255, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.