Rw6G044980

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000834
Physical Location & Seq
Reverse (-)
68099480 .. 68105246
5767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G044980.1

Sequence Viewer

Length: 642 bp
ATGCACAAGACCGGACATCTCATTTGCTATAAACTTGTTAATGCATGCCAGCCTGATGCTGCTGAGTCCTTCACCAACCTCATGTGGCCTCAAGGGAGCTCTTCTTTTTGCTTCGGCGTGGGAAAGCACTACACTTCGCACGTGGAGAACACCACCAGCATCATGCGTTTCATGAAGTACAAAATTCCTCCGAACAGCCTACCATGTAGTTCCGCCCCTGGCGACACATCGTCAGCTGCCATTGGTCTTGTGCCTCACACAGACAAGAGTGCCCTAACAGTTCTGTGCCCGAACGAAGTGCAGGGTCTCGAGATCCTCACCAAACAGGGAAAATGGATCGAAGTCACAGTCCCCGAACGAGCCGTTGTGGTCTTCGTTGGGGATGCACTCAAGGCTTGGAGCAACGTGAGGCTTCATGCAGTGACTCACAGGCATGGGCCTAGGGATAAAGATATTAAGGAAGACAGATACTCTTTTGCGGTGTTTGGGTGGCCGAAGGAGGAGTTCATGATTGAAGTCCCGAATAAGCTTATCGACAATGAGCATCCCCTGCTGTACCGGCCGTTCAACTTTGCCGAGTACTTTACCTACTTCACGGCCAATATTCGTGATGATGCACTGGAACTCTATGCTGGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

213

Amino Acids

23.98

Weight (kDa)

6.49

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 76 - 162 3.4e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 213, 479
AclWI GGATC 2 cut(s) 307, 344
AcoI YGGCCR 3 cut(s) 491, 560, 597
AcsI RAATTY 1 cut(s) 183
AcvI CACGTG 1 cut(s) 142
AfaI GTAC 3 cut(s) 179, 557, 581
AgsI TTSAA 2 cut(s) 515, 568
AhdI GACNNNNNGTC 1 cut(s) 229
AjnI CCWGG 1 cut(s) 217
AluBI AGCT 3 cut(s) 99, 236, 529
AluI AGCT 3 cut(s) 99, 236, 529
Alw21I GWGCWC 1 cut(s) 101
Alw26I GTCTC 1 cut(s) 311
AlwI GGATC 2 cut(s) 307, 344
Ama87I CYCGRG 1 cut(s) 308
AoxI GGCC 5 cut(s) 86, 437, 491, 560, 597
ApeKI GCWGC 2 cut(s) 59, 236
ApoI RAATTY 1 cut(s) 183
ArsI GACNNNNNNTTYG 3 cut(s) 38, 333, 365
AspA2I CCTAGG 1 cut(s) 440
AspS9I GGNCC 1 cut(s) 437
AsuHPI GGTGA 2 cut(s) 64, 310
AvaI CYCGRG 1 cut(s) 308
AvrII CCTAGG 1 cut(s) 440
BaeGI GKGCMC 2 cut(s) 274, 290
BanII GRGCYC 1 cut(s) 101
BbrPI CACGTG 1 cut(s) 142
BbsI GAAGAC 2 cut(s) 364, 468
Bbv12I GWGCWC 1 cut(s) 101
BbvI GCAGC 2 cut(s) 46, 223
BceAI ACGGC 3 cut(s) 347, 547, 612
BcgI CGANNNNNNTGC 2 cut(s) 280, 314
BciT130I CCWGG 1 cut(s) 219
BcoDI GTCTC 1 cut(s) 311
BfaI CTAG 1 cut(s) 441
BisI GCNGC 2 cut(s) 60, 237
BlnI CCTAGG 1 cut(s) 440
BlsI GCNGC 2 cut(s) 61, 238
BmcAI AGTACT 1 cut(s) 581
Bme1390I CCNGG 1 cut(s) 219
BmeRI GACNNNNNGTC 1 cut(s) 229
BmeT110I CYCGRG 1 cut(s) 308
BmgT120I GGNCC 1 cut(s) 437
BmrFI CCNGG 1 cut(s) 219
BmsI GCATC 5 cut(s) 46, 168, 373, 553, 604
BpiI GAAGAC 2 cut(s) 364, 468
BpuEI CTTGAG 2 cut(s) 75, 374
BsaAI YACGTR 1 cut(s) 142
BsaI GGTCTC 1 cut(s) 311
BsaJI CCNNGG 2 cut(s) 217, 440
BsaWI WCCGGW 1 cut(s) 11
Bse118I RCCGGY 1 cut(s) 558
Bse1I ACTGG 1 cut(s) 624
BseBI CCWGG 1 cut(s) 219
BseDI CCNNGG 2 cut(s) 217, 440
BseGI GGATG 2 cut(s) 388, 544
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 1 cut(s) 624
BseRI GAGGAG 1 cut(s) 515
BseSI GKGCMC 2 cut(s) 274, 290
BseX3I CGGCCG 1 cut(s) 560
BseXI GCAGC 2 cut(s) 46, 223
BsgI GTGCAG 1 cut(s) 320
Bsh1285I CGRYCG 1 cut(s) 563
BshFI GGCC 5 cut(s) 88, 439, 493, 562, 599
BsiEI CGRYCG 1 cut(s) 563
BsiHKAI GWGCWC 1 cut(s) 101
BsiHKCI CYCGRG 1 cut(s) 308
BsiSI CCGG 2 cut(s) 12, 559
BslFI GGGAC 2 cut(s) 335, 503
BsmAI GTCTC 1 cut(s) 311
BsmFI GGGAC 2 cut(s) 335, 503
BsnI GGCC 5 cut(s) 88, 439, 493, 562, 599
Bso31I GGTCTC 1 cut(s) 311
BsoBI CYCGRG 1 cut(s) 308
Bsp1286I GDGCHC 3 cut(s) 101, 274, 290
Bsp143I GATC 2 cut(s) 312, 336
BspACI CCGC 2 cut(s) 213, 479
BspANI GGCC 5 cut(s) 88, 439, 493, 562, 599
BspCNI CTCAG 1 cut(s) 55
BspHI TCATGA 2 cut(s) 171, 507
BspPI GGATC 2 cut(s) 307, 344
BspQI GCTCTTC 1 cut(s) 106
BspTNI GGTCTC 1 cut(s) 311
BsrFI RCCGGY 1 cut(s) 558
BsrI ACTGG 1 cut(s) 624
BssAI RCCGGY 1 cut(s) 558
BssECI CCNNGG 2 cut(s) 217, 440
BssMI GATC 2 cut(s) 312, 336
BssT1I CCWWGG 1 cut(s) 440
Bst2UI CCWGG 1 cut(s) 219
Bst4CI ACNGT 2 cut(s) 280, 349
Bst6I CTCTTC 1 cut(s) 106
BstAPI GCANNNNNTGC 1 cut(s) 550
BstBAI YACGTR 1 cut(s) 142
BstC8I GCNNGC 2 cut(s) 46, 50
BstDEI CTNAG 1 cut(s) 63
BstF5I GGATG 2 cut(s) 388, 544
BstKTI GATC 2 cut(s) 315, 339
BstMAI GTCTC 1 cut(s) 311
BstMBI GATC 2 cut(s) 312, 336
BstMCI CGRYCG 1 cut(s) 563
BstMWI GCNNNNNNNGC 3 cut(s) 392, 550, 559
BstNI CCWGG 1 cut(s) 219
BstNSI RCATGY 1 cut(s) 48
BstSCI CCNGG 1 cut(s) 217
BstSLI GKGCMC 2 cut(s) 274, 290
BstV1I GCAGC 2 cut(s) 46, 223
BstV2I GAAGAC 2 cut(s) 364, 468
BstX2I RGATCY 1 cut(s) 312
BstYI RGATCY 1 cut(s) 312
BstZI CGGCCG 1 cut(s) 560
BsuRI GGCC 5 cut(s) 88, 439, 493, 562, 599
BtsCI GGATG 2 cut(s) 388, 544
BtsI GCAGTG 1 cut(s) 426
BtsIMutI CAGTG 2 cut(s) 426, 617
Cac8I GCNNGC 2 cut(s) 46, 50
CciI TCATGA 2 cut(s) 171, 507
Cfr10I RCCGGY 1 cut(s) 558
Cfr13I GGNCC 1 cut(s) 437
Csp6I GTAC 3 cut(s) 178, 556, 580
CviAII CATG 8 cut(s) 45, 82, 163, 172, 204, 416, 434, 508
CviQI GTAC 3 cut(s) 178, 556, 580
DdeI CTNAG 1 cut(s) 63
DpnI GATC 2 cut(s) 314, 338
DpnII GATC 2 cut(s) 312, 336
DriI GACNNNNNGTC 1 cut(s) 229
EaeI YGGCCR 3 cut(s) 491, 560, 597
EagI CGGCCG 1 cut(s) 560
Eam1104I CTCTTC 1 cut(s) 106
Eam1105I GACNNNNNGTC 1 cut(s) 229
EarI CTCTTC 1 cut(s) 106
EciI GGCGGA 1 cut(s) 202
Ecl136II GAGCTC 1 cut(s) 99
EclXI CGGCCG 1 cut(s) 560
Eco130I CCWWGG 1 cut(s) 440
Eco24I GRGCYC 1 cut(s) 101
Eco31I GGTCTC 1 cut(s) 311
Eco52I CGGCCG 1 cut(s) 560
Eco53kI GAGCTC 1 cut(s) 99
Eco72I CACGTG 1 cut(s) 142
Eco88I CYCGRG 1 cut(s) 308
EcoICRI GAGCTC 1 cut(s) 99
EcoRII CCWGG 1 cut(s) 217
EcoT14I CCWWGG 1 cut(s) 440
EcoT22I ATGCAT 1 cut(s) 46
EcoT38I GRGCYC 1 cut(s) 101
ErhI CCWWGG 1 cut(s) 440
FaeI CATG 8 cut(s) 48, 85, 166, 175, 207, 419, 437, 511
FaqI GGGAC 2 cut(s) 335, 503
FatI CATG 8 cut(s) 44, 81, 162, 171, 203, 415, 433, 507
Fnu4HI GCNGC 2 cut(s) 60, 237
FokI GGATG 2 cut(s) 395, 531
FriOI GRGCYC 1 cut(s) 101
Fsp4HI GCNGC 2 cut(s) 60, 237
FspBI CTAG 1 cut(s) 441
GluI GCNGC 2 cut(s) 60, 237
HaeIII GGCC 5 cut(s) 88, 439, 493, 562, 599
HapII CCGG 2 cut(s) 12, 559
Hin1II CATG 8 cut(s) 48, 85, 166, 175, 207, 419, 437, 511
HindIII AAGCTT 1 cut(s) 527
HinfI GANTC 2 cut(s) 65, 424
HpaII CCGG 2 cut(s) 12, 559
HphI GGTGA 2 cut(s) 64, 310
Hpy188I TCNGA 1 cut(s) 192
Hpy188III TCNNGA 6 cut(s) 172, 308, 310, 508, 520, 608
HpyAV CCTTC 2 cut(s) 79, 490
HpyCH4III ACNGT 2 cut(s) 280, 349
HpyCH4IV ACGT 2 cut(s) 141, 405
HpyCH4V TGCA 6 cut(s) 4, 44, 301, 386, 419, 617
HpyF10VI GCNNNNNNNGC 3 cut(s) 392, 550, 559
HpyF3I CTNAG 1 cut(s) 63
HpySE526I ACGT 2 cut(s) 141, 405
Hsp92II CATG 8 cut(s) 48, 85, 166, 175, 207, 419, 437, 511
Kzo9I GATC 2 cut(s) 312, 336
LguI GCTCTTC 1 cut(s) 106
LmnI GCTCC 2 cut(s) 96, 399
Lsp1109I GCAGC 2 cut(s) 46, 223
LweI GCATC 5 cut(s) 46, 168, 373, 553, 604
MaeI CTAG 1 cut(s) 441
MaeII ACGT 2 cut(s) 141, 405
MaeIII GTNAC 2 cut(s) 343, 421
MalI GATC 2 cut(s) 314, 338
MboI GATC 2 cut(s) 312, 336
MboII GAAGA 3 cut(s) 93, 364, 473
MflI RGATCY 1 cut(s) 312
MhlI GDGCHC 3 cut(s) 101, 274, 290
MluCI AATT 1 cut(s) 183
MlyI GAGTC 2 cut(s) 74, 418
MnlI CCTC 7 cut(s) 89, 99, 198, 264, 326, 402, 493
Mph1103I ATGCAT 1 cut(s) 46
MseI TTAA 2 cut(s) 39, 456
MslI CAYNNNNRTG 1 cut(s) 432
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 2 cut(s) 12, 559
MspR9I CCNGG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 219
MwoI GCNNNNNNNGC 3 cut(s) 392, 550, 559
NdeII GATC 2 cut(s) 312, 336
NlaIII CATG 8 cut(s) 48, 85, 166, 175, 207, 419, 437, 511
NmeAIII GCCGAG 1 cut(s) 601
NmuCI GTSAC 2 cut(s) 343, 421
NsiI ATGCAT 1 cut(s) 46
NspI RCATGY 1 cut(s) 48
PaeI GCATGC 1 cut(s) 48
PaeR7I CTCGAG 1 cut(s) 308
PagI TCATGA 2 cut(s) 171, 507
PciSI GCTCTTC 1 cut(s) 106
PkrI GCNGC 2 cut(s) 61, 238
PleI GAGTC 2 cut(s) 73, 418
PmaCI CACGTG 1 cut(s) 142
PmlI CACGTG 1 cut(s) 142
PpsI GAGTC 2 cut(s) 73, 418
Ppu21I YACGTR 1 cut(s) 142
Psp124BI GAGCTC 1 cut(s) 101
Psp6I CCWGG 1 cut(s) 217
PspCI CACGTG 1 cut(s) 142
PspGI CCWGG 1 cut(s) 217
PspPI GGNCC 1 cut(s) 437
PsrI GAACNNNNNNTAC 2 cut(s) 548, 580
PsuI RGATCY 1 cut(s) 312
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 3 cut(s) 179, 557, 581
RsaNI GTAC 3 cut(s) 178, 556, 580
RseI CAYNNNNRTG 1 cut(s) 432
SacI GAGCTC 1 cut(s) 101
SapI GCTCTTC 1 cut(s) 106
SaqAI TTAA 2 cut(s) 39, 456
SatI GCNGC 2 cut(s) 60, 237
Sau3AI GATC 2 cut(s) 312, 336
Sau96I GGNCC 1 cut(s) 437
ScaI AGTACT 1 cut(s) 581
SchI GAGTC 2 cut(s) 74, 418
ScrFI CCNGG 1 cut(s) 219
SduI GDGCHC 3 cut(s) 101, 274, 290
SetI ASST 7 cut(s) 81, 101, 144, 238, 408, 531, 590
SfaNI GCATC 5 cut(s) 46, 168, 373, 553, 604
Sfr274I CTCGAG 1 cut(s) 308
SlaI CTCGAG 1 cut(s) 308
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 3 cut(s) 90, 308, 389
SmoI CTYRAG 3 cut(s) 90, 308, 389
SphI GCATGC 1 cut(s) 48
Sse9I AATT 1 cut(s) 183
SsiI CCGC 2 cut(s) 213, 479
SspI AATATT 1 cut(s) 604
SspMI CTAG 1 cut(s) 441
SstI GAGCTC 1 cut(s) 101
StyD4I CCNGG 1 cut(s) 217
StyI CCWWGG 1 cut(s) 440
TaaI ACNGT 2 cut(s) 280, 349
TaiI ACGT 2 cut(s) 144, 408
TaqI TCGA 3 cut(s) 309, 339, 534
TasI AATT 1 cut(s) 183
TatI WGTACW 2 cut(s) 177, 579
Tru1I TTAA 2 cut(s) 39, 456
Tru9I TTAA 2 cut(s) 39, 456
TscAI CASTG 2 cut(s) 426, 624
TseFI GTSAC 2 cut(s) 343, 421
TseI GCWGC 2 cut(s) 59, 236
Tsp45I GTSAC 2 cut(s) 343, 421
TspDTI ATGAA 4 cut(s) 160, 188, 404, 496
TspRI CASTG 2 cut(s) 426, 624
XapI RAATTY 1 cut(s) 183
XceI RCATGY 1 cut(s) 48
XhoI CTCGAG 1 cut(s) 308
XmaJI CCTAGG 1 cut(s) 440
XspI CTAG 1 cut(s) 441
ZrmI AGTACT 1 cut(s) 581
Zsp2I ATGCAT 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.