RLG00000010287

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000834
Physical Location & Seq
Forward (+)
220634 .. 226493
5860 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010287

Sequence Viewer

Length: 984 bp
ATGGGTGAGGAGGCTGATCATGATGAAGCACTTCTACTCCCAATTCTTGAATTCTCGAAGGAGTGTTTGGTCCCAAAGCAAGAGAGGAGTGAGGAATGGAGAGCTATGAGCATAAAAGTGAGAGAGGCGTGTGAGAGTCATGGCTGCTTTCTACTGTTGGTGCAGGAAGAGATGATCCCGATGCGTTTACGTGAAGAAATGGTGGCGGCCATGAAAGCTTTGTTCGATCTCCCTGAAGAAACCAAGCAGAAGCACACAAGCCTCAGGGCTTACCAGAGCTACCAAGGCAAGTGCCCCATCATTCCCTTGAACGAAAGCTTCGGAATTGACAATACATGCCAGCCTGATGCAGCTGAGTCCTTCACCAACCTCATGTGGCCTCAAGGAAGCTCTTCTTTTTGTGAGATAATGAAATCTATGAGCTCAAAGATGATGGCGTTGAACTTCACCATCCTCGAAATGCTTTTAGAGAGCTTCGGCGCAGGAAAGCATTACACTTCGCATGTGGAGAACACCACCAGCATCATGCGTTTCATGAAGTACAAAACTCCTCCGAGCAACGACTGCGACACATCGTCAGCTGCCGTTGGCCTGGTGCCTCACACAGACAAGAGTGCCCTAACAGTTCTGTGCCAGAACGAAGTGCAGGGTCTGGAGATCCTCACCAAACAGGGCCAATGGATCGAAGTCACAGTCCCCGAACGAGCCGTTGTGGTCTTCGTTGGGGATGCACTCAAGGCTTGGAGCAACGCGAGGCTTCATGCAGTGACTCACAGGGTGATGATGAGTAAAGATATTAAGGAAGACAGATACTCTTTTGCGGTATTTGGGTGGCCGAAGGAGGAGTTCATGATTGAAGTCCCGAATGAGCTTATCGACAATGAGCATCCTCTGCTGTACCGGCCGTTCAACTTTGCCGAGTACTTTACCTACTTCACGGCCAATATTCGTGATGATGCACTGGAACTCTATGCTGGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

328

Amino Acids

37.28

Weight (kDa)

5.0

Isoelectric Point (pI)

46.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 14 - 96 1.3e-10 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 180 - 276 1.4e-19 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 595
AccII CGCG 1 cut(s) 752
AciI CCGC 2 cut(s) 206, 821
AclWI GGATC 3 cut(s) 169, 652, 689
AcoI YGGCCR 4 cut(s) 207, 833, 902, 939
AcsI RAATTY 1 cut(s) 50
AcuI CTGAAG 1 cut(s) 255
AdeI CACNNNGTG 1 cut(s) 778
AfaI GTAC 3 cut(s) 542, 899, 923
AgsI TTSAA 5 cut(s) 50, 310, 442, 857, 910
AhdI GACNNNNNGTC 1 cut(s) 574
AjnI CCWGG 1 cut(s) 591
AjuI GAANNNNNNNTTGG 2 cut(s) 50, 82
Alw21I GWGCWC 1 cut(s) 425
AlwI GGATC 3 cut(s) 169, 652, 689
AlwNI CAGNNNCTG 1 cut(s) 652
AoxI GGCC 7 cut(s) 207, 377, 589, 673, 833, 902, 939
ApeKI GCWGC 3 cut(s) 144, 350, 581
ApoI RAATTY 1 cut(s) 50
ArsI GACNNNNNNTTYG 2 cut(s) 678, 710
Asp700I GAANNNNTTC 2 cut(s) 30, 391
AspLEI GCGC 1 cut(s) 482
AspS9I GGNCC 2 cut(s) 70, 673
AsuHPI GGTGA 5 cut(s) 17, 355, 439, 655, 790
AvaII GGWCC 1 cut(s) 70
AxyI CCTNAGG 1 cut(s) 263
BaeGI GKGCMC 2 cut(s) 296, 619
BanI GGYRCC 1 cut(s) 595
BanII GRGCYC 1 cut(s) 425
BarI GAAGNNNNNNTAC 2 cut(s) 18, 50
BbsI GAAGAC 2 cut(s) 709, 810
Bbv12I GWGCWC 1 cut(s) 425
BbvI GCAGC 3 cut(s) 131, 362, 568
BccI CCATC 3 cut(s) 305, 427, 458
BceAI ACGGC 4 cut(s) 569, 692, 889, 954
BciT130I CCWGG 1 cut(s) 593
BclI TGATCA 1 cut(s) 16
BisI GCNGC 4 cut(s) 145, 207, 351, 582
BlsI GCNGC 4 cut(s) 146, 208, 352, 583
BmcAI AGTACT 1 cut(s) 923
Bme1390I CCNGG 1 cut(s) 593
Bme18I GGWCC 1 cut(s) 70
BmeRI GACNNNNNGTC 1 cut(s) 574
BmgT120I GGNCC 2 cut(s) 70, 673
BmiI GGNNCC 2 cut(s) 72, 597
BmrFI CCNGG 1 cut(s) 593
BmsI GCATC 6 cut(s) 171, 337, 531, 718, 895, 946
BpiI GAAGAC 2 cut(s) 709, 810
BpmI CTGGAG 1 cut(s) 674
BpuEI CTTGAG 2 cut(s) 366, 719
BsaAI YACGTR 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 283
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bse118I RCCGGY 1 cut(s) 900
Bse1I ACTGG 1 cut(s) 966
Bse21I CCTNAGG 1 cut(s) 263
BseBI CCWGG 1 cut(s) 593
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 3 cut(s) 450, 733, 886
BseMII CTCAG 2 cut(s) 277, 345
BseNI ACTGG 1 cut(s) 966
BseRI GAGGAG 4 cut(s) 23, 100, 540, 857
BseSI GKGCMC 2 cut(s) 296, 619
BseX3I CGGCCG 1 cut(s) 902
BseXI GCAGC 3 cut(s) 131, 362, 568
BsgI GTGCAG 2 cut(s) 182, 665
Bsh1236I CGCG 1 cut(s) 752
Bsh1285I CGRYCG 1 cut(s) 905
BshFI GGCC 7 cut(s) 209, 379, 591, 675, 835, 904, 941
BshNI GGYRCC 1 cut(s) 595
BsiEI CGRYCG 1 cut(s) 905
BsiHKAI GWGCWC 1 cut(s) 425
BsiSI CCGG 1 cut(s) 901
BslFI GGGAC 3 cut(s) 56, 680, 845
BsmFI GGGAC 3 cut(s) 56, 680, 845
BsnI GGCC 7 cut(s) 209, 379, 591, 675, 835, 904, 941
Bsp1286I GDGCHC 3 cut(s) 296, 425, 619
Bsp143I GATC 5 cut(s) 16, 174, 226, 657, 681
BspACI CCGC 2 cut(s) 206, 821
BspANI GGCC 7 cut(s) 209, 379, 591, 675, 835, 904, 941
BspCNI CTCAG 2 cut(s) 276, 346
BspFNI CGCG 1 cut(s) 752
BspHI TCATGA 3 cut(s) 19, 534, 849
BspLI GGNNCC 2 cut(s) 72, 597
BspPI GGATC 3 cut(s) 169, 652, 689
BspQI GCTCTTC 1 cut(s) 397
BspT107I GGYRCC 1 cut(s) 595
BsrFI RCCGGY 1 cut(s) 900
BsrI ACTGG 1 cut(s) 966
BssAI RCCGGY 1 cut(s) 900
BssECI CCNNGG 1 cut(s) 283
BssMI GATC 5 cut(s) 16, 174, 226, 657, 681
BssT1I CCWWGG 1 cut(s) 283
Bst2UI CCWGG 1 cut(s) 593
Bst4CI ACNGT 3 cut(s) 156, 625, 694
Bst6I CTCTTC 2 cut(s) 162, 397
BstAPI GCANNNNNTGC 2 cut(s) 564, 892
BstBAI YACGTR 1 cut(s) 191
BstC8I GCNNGC 1 cut(s) 341
BstDEI CTNAG 2 cut(s) 263, 354
BstF5I GGATG 3 cut(s) 450, 733, 886
BstFNI CGCG 1 cut(s) 752
BstHHI GCGC 1 cut(s) 482
BstKTI GATC 5 cut(s) 19, 177, 229, 660, 684
BstMBI GATC 5 cut(s) 16, 174, 226, 657, 681
BstMCI CGRYCG 1 cut(s) 905
BstMWI GCNNNNNNNGC 6 cut(s) 215, 285, 564, 737, 892, 901
BstNI CCWGG 1 cut(s) 593
BstNSI RCATGY 2 cut(s) 339, 506
BstSCI CCNGG 1 cut(s) 591
BstSLI GKGCMC 2 cut(s) 296, 619
BstUI CGCG 1 cut(s) 752
BstV1I GCAGC 3 cut(s) 131, 362, 568
BstV2I GAAGAC 2 cut(s) 709, 810
BstX2I RGATCY 1 cut(s) 657
BstYI RGATCY 1 cut(s) 657
BstZI CGGCCG 1 cut(s) 902
Bsu36I CCTNAGG 1 cut(s) 263
BsuRI GGCC 7 cut(s) 209, 379, 591, 675, 835, 904, 941
BtsCI GGATG 3 cut(s) 450, 733, 886
BtsI GCAGTG 1 cut(s) 771
BtsIMutI CAGTG 2 cut(s) 771, 959
Cac8I GCNNGC 1 cut(s) 341
CaiI CAGNNNCTG 1 cut(s) 652
CciI TCATGA 3 cut(s) 19, 534, 849
CfoI GCGC 1 cut(s) 482
Cfr10I RCCGGY 1 cut(s) 900
Cfr13I GGNCC 2 cut(s) 70, 673
Csp6I GTAC 3 cut(s) 541, 898, 922
CviQI GTAC 3 cut(s) 541, 898, 922
DdeI CTNAG 2 cut(s) 263, 354
DpnI GATC 5 cut(s) 18, 176, 228, 659, 683
DpnII GATC 5 cut(s) 16, 174, 226, 657, 681
DraIII CACNNNGTG 1 cut(s) 778
DriI GACNNNNNGTC 1 cut(s) 574
EaeI YGGCCR 4 cut(s) 207, 833, 902, 939
EagI CGGCCG 1 cut(s) 902
Eam1104I CTCTTC 2 cut(s) 162, 397
Eam1105I GACNNNNNGTC 1 cut(s) 574
EarI CTCTTC 2 cut(s) 162, 397
Ecl136II GAGCTC 1 cut(s) 423
EclXI CGGCCG 1 cut(s) 902
Eco130I CCWWGG 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 425
Eco47I GGWCC 1 cut(s) 70
Eco52I CGGCCG 1 cut(s) 902
Eco53kI GAGCTC 1 cut(s) 423
Eco57I CTGAAG 1 cut(s) 255
Eco81I CCTNAGG 1 cut(s) 263
EcoICRI GAGCTC 1 cut(s) 423
EcoRI GAATTC 1 cut(s) 50
EcoRII CCWGG 1 cut(s) 591
EcoT14I CCWWGG 1 cut(s) 283
EcoT38I GRGCYC 1 cut(s) 425
ErhI CCWWGG 1 cut(s) 283
FalI AAGNNNNNCTT 2 cut(s) 379, 411
FaqI GGGAC 3 cut(s) 56, 680, 845
FbaI TGATCA 1 cut(s) 16
Fnu4HI GCNGC 4 cut(s) 145, 207, 351, 582
FokI GGATG 3 cut(s) 437, 740, 873
FriOI GRGCYC 1 cut(s) 425
Fsp4HI GCNGC 4 cut(s) 145, 207, 351, 582
GlaI GCGC 1 cut(s) 481
GluI GCNGC 4 cut(s) 145, 207, 351, 582
GsuI CTGGAG 1 cut(s) 674
HaeIII GGCC 7 cut(s) 209, 379, 591, 675, 835, 904, 941
HapII CCGG 1 cut(s) 901
HhaI GCGC 1 cut(s) 482
Hin6I GCGC 1 cut(s) 480
HinP1I GCGC 1 cut(s) 480
HindIII AAGCTT 2 cut(s) 216, 316
HinfI GANTC 3 cut(s) 136, 356, 769
HpaII CCGG 1 cut(s) 901
HphI GGTGA 5 cut(s) 17, 355, 439, 655, 790
Hpy166II GTNNAC 1 cut(s) 188
Hpy188I TCNGA 2 cut(s) 323, 555
Hpy188III TCNNGA 9 cut(s) 20, 47, 55, 178, 535, 653, 850, 862, 950
Hpy8I GTNNAC 1 cut(s) 188
HpyAV CCTTC 3 cut(s) 52, 370, 832
HpyCH4III ACNGT 3 cut(s) 156, 625, 694
HpyCH4IV ACGT 1 cut(s) 190
HpyCH4V TGCA 6 cut(s) 163, 350, 646, 731, 764, 959
HpyF10VI GCNNNNNNNGC 6 cut(s) 215, 285, 564, 737, 892, 901
HpyF3I CTNAG 2 cut(s) 263, 354
HpySE526I ACGT 1 cut(s) 190
HspAI GCGC 1 cut(s) 480
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 5 cut(s) 16, 174, 226, 657, 681
LguI GCTCTTC 1 cut(s) 397
LmnI GCTCC 1 cut(s) 744
Lsp1109I GCAGC 3 cut(s) 131, 362, 568
LweI GCATC 6 cut(s) 171, 337, 531, 718, 895, 946
MaeII ACGT 1 cut(s) 190
MaeIII GTNAC 2 cut(s) 688, 766
MalI GATC 5 cut(s) 18, 176, 228, 659, 683
MboI GATC 5 cut(s) 16, 174, 226, 657, 681
MboII GAAGA 6 cut(s) 179, 206, 248, 384, 709, 815
MflI RGATCY 1 cut(s) 657
MhlI GDGCHC 3 cut(s) 296, 425, 619
MluCI AATT 3 cut(s) 42, 50, 324
MlyI GAGTC 3 cut(s) 145, 365, 763
MroXI GAANNNNTTC 2 cut(s) 30, 391
MseI TTAA 1 cut(s) 798
MslI CAYNNNNRTG 1 cut(s) 116
MspA1I CMGCKG 2 cut(s) 353, 581
MspI CCGG 1 cut(s) 901
MspR9I CCNGG 1 cut(s) 593
MvaI CCWGG 1 cut(s) 593
MvnI CGCG 1 cut(s) 752
MwoI GCNNNNNNNGC 6 cut(s) 215, 285, 564, 737, 892, 901
NdeII GATC 5 cut(s) 16, 174, 226, 657, 681
NlaIV GGNNCC 2 cut(s) 72, 597
NmeAIII GCCGAG 1 cut(s) 943
NmuCI GTSAC 2 cut(s) 688, 766
NspI RCATGY 2 cut(s) 339, 506
PagI TCATGA 3 cut(s) 19, 534, 849
PciSI GCTCTTC 1 cut(s) 397
PdmI GAANNNNTTC 2 cut(s) 30, 391
PkrI GCNGC 4 cut(s) 146, 208, 352, 583
PleI GAGTC 3 cut(s) 144, 364, 763
PpsI GAGTC 3 cut(s) 144, 364, 763
Ppu21I YACGTR 1 cut(s) 191
Psp124BI GAGCTC 1 cut(s) 425
Psp6I CCWGG 1 cut(s) 591
PspGI CCWGG 1 cut(s) 591
PspN4I GGNNCC 2 cut(s) 72, 597
PspPI GGNCC 2 cut(s) 70, 673
PsrI GAACNNNNNNTAC 2 cut(s) 890, 922
PstNI CAGNNNCTG 1 cut(s) 652
PsuI RGATCY 1 cut(s) 657
PvuII CAGCTG 2 cut(s) 353, 581
RsaI GTAC 3 cut(s) 542, 899, 923
RsaNI GTAC 3 cut(s) 541, 898, 922
RseI CAYNNNNRTG 1 cut(s) 116
SacI GAGCTC 1 cut(s) 425
SapI GCTCTTC 1 cut(s) 397
SaqAI TTAA 1 cut(s) 798
SatI GCNGC 4 cut(s) 145, 207, 351, 582
Sau3AI GATC 5 cut(s) 16, 174, 226, 657, 681
Sau96I GGNCC 2 cut(s) 70, 673
ScaI AGTACT 1 cut(s) 923
SchI GAGTC 3 cut(s) 145, 365, 763
ScrFI CCNGG 1 cut(s) 593
SduI GDGCHC 3 cut(s) 296, 425, 619
SfaNI GCATC 6 cut(s) 171, 337, 531, 718, 895, 946
SinI GGWCC 1 cut(s) 70
SmiMI CAYNNNNRTG 1 cut(s) 116
SmlI CTYRAG 2 cut(s) 381, 734
SmoI CTYRAG 2 cut(s) 381, 734
Sse9I AATT 3 cut(s) 42, 50, 324
SsiI CCGC 2 cut(s) 206, 821
SspI AATATT 1 cut(s) 946
SstI GAGCTC 1 cut(s) 425
StyD4I CCNGG 1 cut(s) 591
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 3 cut(s) 156, 625, 694
TaiI ACGT 1 cut(s) 193
TaqI TCGA 5 cut(s) 56, 225, 456, 684, 876
TasI AATT 3 cut(s) 42, 50, 324
TatI WGTACW 2 cut(s) 540, 921
TauI GCSGC 1 cut(s) 209
Tru1I TTAA 1 cut(s) 798
Tru9I TTAA 1 cut(s) 798
TscAI CASTG 2 cut(s) 771, 966
TseFI GTSAC 2 cut(s) 688, 766
TseI GCWGC 3 cut(s) 144, 350, 581
Tsp45I GTSAC 2 cut(s) 688, 766
TspDTI ATGAA 7 cut(s) 39, 227, 425, 523, 551, 749, 838
TspRI CASTG 2 cut(s) 771, 966
VpaK11BI GGWCC 1 cut(s) 70
XapI RAATTY 1 cut(s) 50
XceI RCATGY 2 cut(s) 339, 506
XmnI GAANNNNTTC 2 cut(s) 30, 391
ZrmI AGTACT 1 cut(s) 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.