RLG00000032460

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000834
Physical Location & Seq
Reverse (-)
15441187 .. 15442396
1210 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032460

Sequence Viewer

Length: 840 bp
ATGGTTGCTTTCTGCGTGCGGGAAGAGACAATCCCCATCAACTTACGTGAAGAAATGGTGATGGCCATGAAGGGTTTGTTCGATCTCCCTGAAGAGACCAAGCAGAAGCACAAAAGTACGAACTCTTTCCGTGACTACCAAGGCAAATCCCCCAACTTTCCATTGAGCGAAAGCTTTGGAATTGATAGTTCAGACCAGATTGATGCAGCTCAAGCCTTCATCAACCTCATGTGGCCTCAAGGGAACCCTGCGTTTTGTGAGAAAATGAAATCAATGAGCTCAAAGCTGATGGATTTGAACTTCACCATTGTCAAAATGATTTTTGAGAGCTTTGGCATTGAGAAGCACTACAAATTGCACGAGGAGAGCACCACCAGCCTTTTGCGTTTGATGAAGTACTACAAACTCCCCACTAGTACCCAATCTGACGACAAAAACGCAGCATCATCGGCTGTCGTCGGTCTAGAGATTCTCACCAAACAAGGCACTTGGGTTCAAGTTACTATCCCAAAAGGAGCTGTTGTGGTCTTTGTTGGCGACGCATTCAAGGTTTGGAGCAATGGTAGACTTCATGCTTCAAGACAGAGAGTGATCATGAGCGGTAGTAGTACTGTAGACAACAAATCAGAAGACATGTACTCTTGTGCGTTGTTTGGATTGCCGGAGGAGGAGTCGATGATCCAACCGCAAAATGAGCTTGTAGACATGGAGCATCCTCTGTTGTATCGGCCAATATTCAACCTCGCCGGATTCTTTAAGTACATTACCACCAATATTTATGCTGTTTCTGATAATCCGCTTAGAGATTATGCTGGAATTGAACTAGTACTGCATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

280

Amino Acids

31.6

Weight (kDa)

5.59

Isoelectric Point (pI)

48.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 4 - 64 2.9e-06 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 150 - 199 2.8e-07 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 600
AccI GTMKAC 3 cut(s) 565, 615, 702
AciI CCGC 4 cut(s) 19, 600, 686, 797
AclWI GGATC 1 cut(s) 673
AcoI YGGCCR 2 cut(s) 63, 728
AcuI CTGAAG 1 cut(s) 111
AfaI GTAC 7 cut(s) 118, 398, 418, 610, 638, 761, 828
AflIII ACRYGT 1 cut(s) 633
AgsI TTSAA 6 cut(s) 298, 497, 547, 579, 739, 821
AhlI ACTAGT 2 cut(s) 413, 823
AluBI AGCT 7 cut(s) 174, 209, 279, 286, 330, 518, 697
AluI AGCT 7 cut(s) 174, 209, 279, 286, 330, 518, 697
Alw21I GWGCWC 2 cut(s) 281, 371
Alw26I GTCTC 2 cut(s) 20, 89
AlwI GGATC 1 cut(s) 673
AoxI GGCC 3 cut(s) 63, 233, 728
ApeKI GCWGC 2 cut(s) 206, 440
Asp700I GAANNNNTTC 1 cut(s) 125
AsuHPI GGTGA 3 cut(s) 70, 295, 466
BalI TGGCCA 1 cut(s) 65
BanII GRGCYC 1 cut(s) 281
BauI CACGAG 1 cut(s) 359
BbsI GAAGAC 1 cut(s) 636
Bbv12I GWGCWC 2 cut(s) 281, 371
BbvI GCAGC 2 cut(s) 218, 452
BccI CCATC 3 cut(s) 44, 55, 283
BcgI CGANNNNNNTGC 2 cut(s) 429, 463
BclI TGATCA 1 cut(s) 591
BcoDI GTCTC 2 cut(s) 20, 89
BcuI ACTAGT 2 cut(s) 413, 823
BfaI CTAG 3 cut(s) 414, 464, 824
BfmI CTRYAG 1 cut(s) 612
BisI GCNGC 2 cut(s) 207, 441
BlsI GCNGC 2 cut(s) 208, 442
BmcAI AGTACT 3 cut(s) 398, 610, 828
BmiI GGNNCC 1 cut(s) 245
BmsI GCATC 3 cut(s) 193, 452, 721
BpiI GAAGAC 1 cut(s) 636
BpuEI CTTGAG 2 cut(s) 195, 222
BsaAI YACGTR 1 cut(s) 47
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 1 cut(s) 139
BsaXI ACNNNNNCTCC 6 cut(s) 507, 537, 547, 577, 656, 686
Bse3DI GCAATG 1 cut(s) 565
BseDI CCNNGG 1 cut(s) 139
BseGI GGATG 1 cut(s) 712
BseMI GCAATG 1 cut(s) 565
BseRI GAGGAG 3 cut(s) 377, 680, 683
BseXI GCAGC 2 cut(s) 218, 452
BshFI GGCC 3 cut(s) 65, 235, 730
BsiHKAI GWGCWC 2 cut(s) 281, 371
BsiSI CCGG 2 cut(s) 662, 747
BsmAI GTCTC 2 cut(s) 20, 89
BsmI GAATGC 1 cut(s) 542
BsnI GGCC 3 cut(s) 65, 235, 730
Bso31I GGTCTC 1 cut(s) 89
Bsp1286I GDGCHC 2 cut(s) 281, 371
Bsp143I GATC 3 cut(s) 82, 591, 678
BspACI CCGC 4 cut(s) 19, 600, 686, 797
BspANI GGCC 3 cut(s) 65, 235, 730
BspHI TCATGA 1 cut(s) 594
BspLI GGNNCC 1 cut(s) 245
BspPI GGATC 1 cut(s) 673
BspTNI GGTCTC 1 cut(s) 89
BsrBI CCGCTC 1 cut(s) 600
BsrDI GCAATG 1 cut(s) 565
BssECI CCNNGG 1 cut(s) 139
BssMI GATC 3 cut(s) 82, 591, 678
BssSI CACGAG 1 cut(s) 359
BssT1I CCWWGG 1 cut(s) 139
Bst2BI CACGAG 1 cut(s) 359
Bst4CI ACNGT 1 cut(s) 613
Bst6I CTCTTC 2 cut(s) 18, 87
BstBAI YACGTR 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 17
BstDEI CTNAG 1 cut(s) 800
BstF5I GGATG 1 cut(s) 712
BstKTI GATC 3 cut(s) 85, 594, 681
BstMAI GTCTC 2 cut(s) 20, 89
BstMBI GATC 3 cut(s) 82, 591, 678
BstMWI GCNNNNNNNGC 4 cut(s) 212, 375, 449, 694
BstNSI RCATGY 1 cut(s) 637
BstSFI CTRYAG 1 cut(s) 612
BstV1I GCAGC 2 cut(s) 218, 452
BstV2I GAAGAC 1 cut(s) 636
BsuRI GGCC 3 cut(s) 65, 235, 730
BtsCI GGATG 1 cut(s) 712
Cac8I GCNNGC 1 cut(s) 17
CciI TCATGA 1 cut(s) 594
CseI GACGC 1 cut(s) 548
Csp6I GTAC 7 cut(s) 117, 397, 417, 609, 637, 760, 827
CviAII CATG 6 cut(s) 67, 229, 572, 595, 634, 706
CviQI GTAC 7 cut(s) 117, 397, 417, 609, 637, 760, 827
DdeI CTNAG 1 cut(s) 800
DpnI GATC 3 cut(s) 84, 593, 680
DpnII GATC 3 cut(s) 82, 591, 678
EaeI YGGCCR 2 cut(s) 63, 728
Eam1104I CTCTTC 2 cut(s) 18, 87
EarI CTCTTC 2 cut(s) 18, 87
Ecl136II GAGCTC 1 cut(s) 279
Eco130I CCWWGG 1 cut(s) 139
Eco24I GRGCYC 1 cut(s) 281
Eco31I GGTCTC 1 cut(s) 89
Eco53kI GAGCTC 1 cut(s) 279
Eco57I CTGAAG 1 cut(s) 111
EcoICRI GAGCTC 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 139
EcoT38I GRGCYC 1 cut(s) 281
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 6 cut(s) 70, 232, 575, 598, 637, 709
FaiI YATR 9 cut(s) 68, 230, 573, 596, 635, 707, 780, 810, 834
FatI CATG 6 cut(s) 66, 228, 571, 594, 633, 705
FauI CCCGC 1 cut(s) 12
FbaI TGATCA 1 cut(s) 591
FblI GTMKAC 3 cut(s) 565, 615, 702
Fnu4HI GCNGC 2 cut(s) 207, 441
FokI GGATG 1 cut(s) 699
FriOI GRGCYC 1 cut(s) 281
Fsp4HI GCNGC 2 cut(s) 207, 441
FspBI CTAG 3 cut(s) 414, 464, 824
GluI GCNGC 2 cut(s) 207, 441
HaeIII GGCC 3 cut(s) 65, 235, 730
HapII CCGG 2 cut(s) 662, 747
HgaI GACGC 1 cut(s) 548
Hin1II CATG 6 cut(s) 70, 232, 575, 598, 637, 709
HindIII AAGCTT 1 cut(s) 172
HinfI GANTC 3 cut(s) 469, 671, 750
HpaII CCGG 2 cut(s) 662, 747
HphI GGTGA 3 cut(s) 70, 295, 466
Hpy166II GTNNAC 3 cut(s) 566, 616, 703
Hpy188I TCNGA 4 cut(s) 193, 427, 628, 790
Hpy188III TCNNGA 3 cut(s) 464, 579, 595
Hpy8I GTNNAC 3 cut(s) 566, 616, 703
Hpy99I CGWCG 2 cut(s) 461, 542
HpyAV CCTTC 2 cut(s) 64, 226
HpyCH4III ACNGT 1 cut(s) 613
HpyCH4IV ACGT 1 cut(s) 46
HpyCH4V TGCA 3 cut(s) 206, 358, 832
HpyF10VI GCNNNNNNNGC 4 cut(s) 212, 375, 449, 694
HpyF3I CTNAG 1 cut(s) 800
HpySE526I ACGT 1 cut(s) 46
Hsp92II CATG 6 cut(s) 70, 232, 575, 598, 637, 709
Ksp22I TGATCA 1 cut(s) 591
Kzo9I GATC 3 cut(s) 82, 591, 678
LmnI GCTCC 3 cut(s) 515, 555, 709
LpnPI CCDG 7 cut(s) 102, 209, 261, 388, 675, 760, 798
Lsp1109I GCAGC 2 cut(s) 218, 452
LweI GCATC 3 cut(s) 193, 452, 721
MaeI CTAG 3 cut(s) 414, 464, 824
MaeII ACGT 1 cut(s) 46
MaeIII GTNAC 2 cut(s) 131, 499
MalI GATC 3 cut(s) 84, 593, 680
MbiI CCGCTC 1 cut(s) 600
MboI GATC 3 cut(s) 82, 591, 678
MboII GAAGA 4 cut(s) 35, 62, 104, 641
MhlI GDGCHC 2 cut(s) 281, 371
MlsI TGGCCA 1 cut(s) 65
MluCI AATT 4 cut(s) 180, 353, 816, 835
MluNI TGGCCA 1 cut(s) 65
MlyI GAGTC 1 cut(s) 680
MmeI TCCRAC 1 cut(s) 706
MnlI CCTC 7 cut(s) 236, 246, 355, 658, 661, 726, 752
Mox20I TGGCCA 1 cut(s) 65
MroXI GAANNNNTTC 1 cut(s) 125
MscI TGGCCA 1 cut(s) 65
MseI TTAA 1 cut(s) 756
Msp20I TGGCCA 1 cut(s) 65
MspI CCGG 2 cut(s) 662, 747
Mva1269I GAATGC 1 cut(s) 542
MwoI GCNNNNNNNGC 4 cut(s) 212, 375, 449, 694
NdeII GATC 3 cut(s) 82, 591, 678
NlaIII CATG 6 cut(s) 70, 232, 575, 598, 637, 709
NlaIV GGNNCC 1 cut(s) 245
NmuCI GTSAC 1 cut(s) 131
NspI RCATGY 1 cut(s) 637
PagI TCATGA 1 cut(s) 594
PciI ACATGT 1 cut(s) 633
PctI GAATGC 1 cut(s) 542
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 2 cut(s) 469, 750
PkrI GCNGC 2 cut(s) 208, 442
PleI GAGTC 1 cut(s) 679
PpsI GAGTC 1 cut(s) 679
Ppu21I YACGTR 1 cut(s) 47
PscI ACATGT 1 cut(s) 633
Psp124BI GAGCTC 1 cut(s) 281
PspN4I GGNNCC 1 cut(s) 245
RsaI GTAC 7 cut(s) 118, 398, 418, 610, 638, 761, 828
RsaNI GTAC 7 cut(s) 117, 397, 417, 609, 637, 760, 827
SacI GAGCTC 1 cut(s) 281
SaqAI TTAA 1 cut(s) 756
SatI GCNGC 2 cut(s) 207, 441
Sau3AI GATC 3 cut(s) 82, 591, 678
ScaI AGTACT 3 cut(s) 398, 610, 828
SchI GAGTC 1 cut(s) 680
SduI GDGCHC 2 cut(s) 281, 371
SfaNI GCATC 3 cut(s) 193, 452, 721
SfcI CTRYAG 1 cut(s) 612
SmlI CTYRAG 2 cut(s) 210, 237
SmoI CTYRAG 2 cut(s) 210, 237
SpeI ACTAGT 2 cut(s) 413, 823
Sse9I AATT 4 cut(s) 180, 353, 816, 835
SsiI CCGC 4 cut(s) 19, 600, 686, 797
SspI AATATT 2 cut(s) 735, 775
SspMI CTAG 3 cut(s) 414, 464, 824
SstI GAGCTC 1 cut(s) 281
StyI CCWWGG 1 cut(s) 139
TaaI ACNGT 1 cut(s) 613
TaiI ACGT 1 cut(s) 49
TaqI TCGA 2 cut(s) 81, 674
TaqII GACCGA 1 cut(s) 449
TasI AATT 4 cut(s) 180, 353, 816, 835
TatI WGTACW 5 cut(s) 396, 608, 636, 759, 826
TfiI GAWTC 2 cut(s) 469, 750
Tru1I TTAA 1 cut(s) 756
Tru9I TTAA 1 cut(s) 756
TseFI GTSAC 1 cut(s) 131
TseI GCWGC 2 cut(s) 206, 440
Tsp45I GTSAC 1 cut(s) 131
TspDTI ATGAA 5 cut(s) 83, 208, 281, 407, 560
TspGWI ACGGA 1 cut(s) 119
XbaI TCTAGA 1 cut(s) 463
XceI RCATGY 1 cut(s) 637
XmiI GTMKAC 3 cut(s) 565, 615, 702
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 3 cut(s) 414, 464, 824
ZrmI AGTACT 3 cut(s) 398, 610, 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.