pycom15g10530

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000834
Physical Location & Seq
Reverse (-)
6990448 .. 6992912
2465 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g10530.1

Sequence Viewer

Length: 1119 bp
ATGGAACCAGCTTCCTATAAATTGAGTGATGCCTTTCTCTTAAAAAACTACGAGAGGAGTCGGAGAGTGGGAGACAGAGAGAGAGAGAGAGAGAGAGAGATGGAGATGGATGATCAAGTACTCCCAATTTTTGTTTTTTCAGAGGGTCTAGTGTTTAAGCAAGGGAGCGAGGAGTGGAAAGCTATGAGTAACAAAGTGAGAGAGGCATGTGAGGATTATGGTTGCTTTCTTTTGCTAGTGAGGGCTGAATCGATCCCCATGAAGTTGCGTGAAGAAATGGTGACGGCCATGAAAGCTTTGTTTGATCTGCCTGAAGAGACCAAGCAGAAGCACACGAGTCCCAAGCCTTATCAAAGCTACGAAGGCAAGAGCCCGGTCATTCCCTTGAACGAAAGCTTTGGCATCGATGACGCTTGCCGCCTAGACGCAGCTCAGGCCTTCACCAACCTCATGTGGCGTCAAGGAAACCCTGCATTTTGTGAGGCAATGAAATCCATGTGCTCCAAGATGATGGACTTGAATATCACCATCCTTTGGATGATCTTAGAGAGCTTTGGTTTGGGGAACCACTATGACACCCATGTCCAAAACACCACCAGTGTTTTTCGTTTCATGAAGTACAAAGCTCCTCCTCCTCCTCCTCCAACTCCAAGCAACATCAACATTAATGTTGGCTCATCAACAACATCACCGTTGGCGTCGCCATCAGCTGCCCTGGGCCTTGTGCCTCATACTGACAAAAATACCCTAACCATTCTGTGCCAAAACGATGTGCAAGGCCTAGAGATTCTGACCAAACAAGGCAAATGGGTTGAAGTGATGATCCCGGAGGGAGCTGTTGTGGTCTTTGTTGGGGATGCACTTAAGGCCTGGAGCAATGGGAGACTTCATGCGGTGACGCACAGGGTTATGATGAGTGGGGACAAAGAGAGGTACTCGTATTCCCTATTTTCTGTGCCAAAAGAGGAGGTGGTGATTGAAGTGCCACAGGAATTTATAGACAAGGAGCATCCTTTGCTGTACAAGCCATTCAACTTTGCCGAATACTTTACATACTTCACGTCCAATGTTCGTGAGGATGCACTCGAATTATATGCTGGAGTTGATATTATGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

373

Amino Acids

42.29

Weight (kDa)

5.29

Isoelectric Point (pI)

48.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 49 - 122 2.3e-12 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 233 - 318 8.2e-21 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 581
AccB7I CCANNNNNTGG 1 cut(s) 534
AciI CCGC 2 cut(s) 418, 893
AclWI GGATC 2 cut(s) 247, 817
AcoI YGGCCR 1 cut(s) 285
AcsI RAATTY 1 cut(s) 992
AcuI CTGAAG 1 cut(s) 333
AcyI GRCGYC 2 cut(s) 457, 698
AfaI GTAC 4 cut(s) 120, 620, 935, 1022
AfiI CCNNNNNNNGG 1 cut(s) 534
AflII CTTAAG 1 cut(s) 863
AgsI TTSAA 5 cut(s) 388, 520, 815, 980, 1033
AjiI CACGTC 1 cut(s) 1062
AjnI CCWGG 2 cut(s) 714, 869
Alw21I GWGCWC 1 cut(s) 503
Alw26I GTCTC 3 cut(s) 66, 311, 877
AlwI GGATC 2 cut(s) 247, 817
AoxI GGCC 5 cut(s) 285, 435, 718, 778, 867
ApeKI GCWGC 2 cut(s) 428, 710
ApoI RAATTY 1 cut(s) 992
AseI ATTAAT 1 cut(s) 666
AspS9I GGNCC 1 cut(s) 718
AsuC2I CCSGG 2 cut(s) 374, 827
AsuHPI GGTGA 6 cut(s) 292, 433, 517, 681, 907, 985
BanII GRGCYC 1 cut(s) 374
BauI CACGAG 1 cut(s) 334
Bbv12I GWGCWC 1 cut(s) 503
BbvI GCAGC 2 cut(s) 440, 697
BccI CCATC 5 cut(s) 94, 100, 505, 536, 712
BceAI ACGGC 1 cut(s) 300
BcgI CGANNNNNNTGC 2 cut(s) 1076, 1110
BciT130I CCWGG 2 cut(s) 716, 871
BclI TGATCA 1 cut(s) 112
BcnI CCSGG 2 cut(s) 374, 827
BcoDI GTCTC 3 cut(s) 66, 311, 877
BfaI CTAG 4 cut(s) 149, 236, 422, 782
BfrI CTTAAG 1 cut(s) 863
BisI GCNGC 3 cut(s) 418, 429, 711
BlsI GCNGC 3 cut(s) 419, 430, 712
BmcAI AGTACT 1 cut(s) 120
Bme1390I CCNGG 4 cut(s) 374, 716, 827, 871
BmgBI CACGTC 1 cut(s) 1062
BmgT120I GGNCC 1 cut(s) 718
BmiI GGNNCC 2 cut(s) 6, 566
BmrFI CCNGG 4 cut(s) 374, 716, 827, 871
BmsI GCATC 5 cut(s) 19, 411, 847, 1018, 1069
BplI GAGNNNNNCTC 2 cut(s) 920, 952
BpmI CTGGAG 2 cut(s) 892, 1119
Bpu10I CCTNAGC 1 cut(s) 432
BpuMI CCSGG 2 cut(s) 374, 827
Bsa29I ATCGAT 2 cut(s) 251, 405
BsaHI GRCGYC 2 cut(s) 457, 698
BsaI GGTCTC 1 cut(s) 311
BsaJI CCNNGG 2 cut(s) 714, 715
BsaXI ACNNNNNCTCC 2 cut(s) 825, 855
Bsc4I CCNNNNNNNGG 1 cut(s) 534
Bse1I ACTGG 1 cut(s) 597
Bse3DI GCAATG 2 cut(s) 492, 883
BseBI CCWGG 2 cut(s) 716, 871
BseCI ATCGAT 2 cut(s) 251, 405
BseDI CCNNGG 2 cut(s) 714, 715
BseGI GGATG 6 cut(s) 115, 528, 543, 862, 1009, 1084
BseLI CCNNNNNNNGG 1 cut(s) 534
BseMI GCAATG 2 cut(s) 492, 883
BseMII CTCAG 1 cut(s) 446
BseNI ACTGG 1 cut(s) 597
BseRI GAGGAG 8 cut(s) 70, 185, 618, 621, 624, 627, 630, 980
BseXI GCAGC 2 cut(s) 440, 697
BshFI GGCC 5 cut(s) 287, 437, 720, 780, 869
BshVI ATCGAT 2 cut(s) 251, 405
BsiHKAI GWGCWC 1 cut(s) 503
BsiSI CCGG 2 cut(s) 374, 827
BslFI GGGAC 2 cut(s) 324, 935
BslI CCNNNNNNNGG 1 cut(s) 534
BsmAI GTCTC 3 cut(s) 66, 311, 877
BsmFI GGGAC 2 cut(s) 324, 935
BsnI GGCC 5 cut(s) 287, 437, 720, 780, 869
Bso31I GGTCTC 1 cut(s) 311
Bsp1286I GDGCHC 2 cut(s) 374, 503
Bsp1407I TGTACA 1 cut(s) 1020
Bsp143I GATC 5 cut(s) 112, 252, 304, 540, 822
BspACI CCGC 2 cut(s) 418, 893
BspANI GGCC 5 cut(s) 287, 437, 720, 780, 869
BspCNI CTCAG 1 cut(s) 445
BspDI ATCGAT 2 cut(s) 251, 405
BspHI TCATGA 1 cut(s) 612
BspLI GGNNCC 2 cut(s) 6, 566
BspPI GGATC 2 cut(s) 247, 817
BspTI CTTAAG 1 cut(s) 863
BspTNI GGTCTC 1 cut(s) 311
BsrDI GCAATG 2 cut(s) 492, 883
BsrGI TGTACA 1 cut(s) 1020
BsrI ACTGG 1 cut(s) 597
BssECI CCNNGG 2 cut(s) 714, 715
BssMI GATC 5 cut(s) 112, 252, 304, 540, 822
BssNI GRCGYC 2 cut(s) 457, 698
BssSI CACGAG 1 cut(s) 334
Bst2BI CACGAG 1 cut(s) 334
Bst2UI CCWGG 2 cut(s) 716, 871
Bst4CI ACNGT 1 cut(s) 693
Bst6I CTCTTC 1 cut(s) 309
BstACI GRCGYC 2 cut(s) 457, 698
BstAFI CTTAAG 1 cut(s) 863
BstAPI GCANNNNNTGC 1 cut(s) 1015
BstAUI TGTACA 1 cut(s) 1020
BstC8I GCNNGC 1 cut(s) 415
BstDEI CTNAG 2 cut(s) 432, 544
BstF5I GGATG 6 cut(s) 115, 528, 543, 862, 1009, 1084
BstKTI GATC 5 cut(s) 115, 255, 307, 543, 825
BstMAI GTCTC 3 cut(s) 66, 311, 877
BstMBI GATC 5 cut(s) 112, 252, 304, 540, 822
BstMWI GCNNNNNNNGC 6 cut(s) 293, 363, 434, 866, 1015, 1024
BstNI CCWGG 2 cut(s) 716, 871
BstNSI RCATGY 1 cut(s) 210
BstSCI CCNGG 4 cut(s) 372, 714, 825, 869
BstV1I GCAGC 2 cut(s) 440, 697
BstXI CCANNNNNNTGG 1 cut(s) 511
Bsu15I ATCGAT 2 cut(s) 251, 405
BsuRI GGCC 5 cut(s) 287, 437, 720, 780, 869
BsuTUI ATCGAT 2 cut(s) 251, 405
BtrI CACGTC 1 cut(s) 1062
BtsCI GGATG 6 cut(s) 115, 528, 543, 862, 1009, 1084
BtsIMutI CAGTG 1 cut(s) 604
Cac8I GCNNGC 1 cut(s) 415
CciI TCATGA 1 cut(s) 612
Cfr13I GGNCC 1 cut(s) 718
ClaI ATCGAT 2 cut(s) 251, 405
CseI GACGC 5 cut(s) 419, 434, 446, 687, 907
Csp6I GTAC 4 cut(s) 119, 619, 934, 1021
CviAII CATG 8 cut(s) 207, 259, 289, 451, 496, 581, 613, 890
CviQI GTAC 4 cut(s) 119, 619, 934, 1021
DdeI CTNAG 2 cut(s) 432, 544
DpnI GATC 5 cut(s) 114, 254, 306, 542, 824
DpnII GATC 5 cut(s) 112, 252, 304, 540, 822
DrdI GACNNNNNNGTC 1 cut(s) 581
DseDI GACNNNNNNGTC 1 cut(s) 581
EaeI YGGCCR 1 cut(s) 285
Eam1104I CTCTTC 1 cut(s) 309
EarI CTCTTC 1 cut(s) 309
Eco147I AGGCCT 3 cut(s) 437, 780, 869
Eco24I GRGCYC 1 cut(s) 374
Eco31I GGTCTC 1 cut(s) 311
Eco57I CTGAAG 1 cut(s) 333
EcoRII CCWGG 2 cut(s) 714, 869
EcoT38I GRGCYC 1 cut(s) 374
FaeI CATG 8 cut(s) 210, 262, 292, 454, 499, 584, 616, 893
FaqI GGGAC 2 cut(s) 324, 935
FatI CATG 8 cut(s) 206, 258, 288, 450, 495, 580, 612, 889
FbaI TGATCA 1 cut(s) 112
Fnu4HI GCNGC 3 cut(s) 418, 429, 711
FokI GGATG 6 cut(s) 122, 515, 550, 869, 996, 1091
FriOI GRGCYC 1 cut(s) 374
Fsp4HI GCNGC 3 cut(s) 418, 429, 711
FspBI CTAG 4 cut(s) 149, 236, 422, 782
GluI GCNGC 3 cut(s) 418, 429, 711
GsuI CTGGAG 2 cut(s) 892, 1119
HaeIII GGCC 5 cut(s) 287, 437, 720, 780, 869
HapII CCGG 2 cut(s) 374, 827
HgaI GACGC 5 cut(s) 419, 434, 446, 687, 907
Hin1I GRCGYC 2 cut(s) 457, 698
Hin1II CATG 8 cut(s) 210, 262, 292, 454, 499, 584, 616, 893
HindIII AAGCTT 2 cut(s) 294, 394
HinfI GANTC 4 cut(s) 58, 248, 337, 787
HpaII CCGG 2 cut(s) 374, 827
HphI GGTGA 6 cut(s) 292, 433, 517, 681, 907, 985
Hpy188I TCNGA 3 cut(s) 63, 142, 792
Hpy188III TCNNGA 2 cut(s) 613, 1073
Hpy99I CGWCG 1 cut(s) 703
HpyAV CCTTC 2 cut(s) 356, 448
HpyCH4III ACNGT 1 cut(s) 693
HpyCH4IV ACGT 1 cut(s) 1061
HpyCH4V TGCA 4 cut(s) 473, 775, 860, 1082
HpyF10VI GCNNNNNNNGC 6 cut(s) 293, 363, 434, 866, 1015, 1024
HpyF3I CTNAG 2 cut(s) 432, 544
HpySE526I ACGT 1 cut(s) 1061
Hsp92I GRCGYC 2 cut(s) 457, 698
Hsp92II CATG 8 cut(s) 210, 262, 292, 454, 499, 584, 616, 893
Ksp22I TGATCA 1 cut(s) 112
Kzo9I GATC 5 cut(s) 112, 252, 304, 540, 822
LmnI GCTCC 6 cut(s) 165, 506, 631, 833, 873, 1006
Lsp1109I GCAGC 2 cut(s) 440, 697
LweI GCATC 5 cut(s) 19, 411, 847, 1018, 1069
MaeI CTAG 4 cut(s) 149, 236, 422, 782
MaeII ACGT 1 cut(s) 1061
MaeIII GTNAC 3 cut(s) 188, 280, 895
MalI GATC 5 cut(s) 114, 254, 306, 542, 824
MboI GATC 5 cut(s) 112, 252, 304, 540, 822
MboII GAAGA 2 cut(s) 284, 326
MhlI GDGCHC 2 cut(s) 374, 503
MluCI AATT 4 cut(s) 20, 126, 992, 1088
MlyI GAGTC 2 cut(s) 67, 346
MmeI TCCRAC 2 cut(s) 41, 668
MseI TTAA 4 cut(s) 41, 156, 666, 864
MspA1I CMGCKG 1 cut(s) 710
MspCI CTTAAG 1 cut(s) 863
MspI CCGG 2 cut(s) 374, 827
MspR9I CCNGG 4 cut(s) 374, 716, 827, 871
MvaI CCWGG 2 cut(s) 716, 871
MwoI GCNNNNNNNGC 6 cut(s) 293, 363, 434, 866, 1015, 1024
NciI CCSGG 2 cut(s) 374, 827
NdeII GATC 5 cut(s) 112, 252, 304, 540, 822
NlaIII CATG 8 cut(s) 210, 262, 292, 454, 499, 584, 616, 893
NlaIV GGNNCC 2 cut(s) 6, 566
NmuCI GTSAC 2 cut(s) 280, 895
NspI RCATGY 1 cut(s) 210
PagI TCATGA 1 cut(s) 612
PasI CCCWGGG 1 cut(s) 715
PceI AGGCCT 3 cut(s) 437, 780, 869
PfeI GAWTC 2 cut(s) 248, 787
PflMI CCANNNNNTGG 1 cut(s) 534
PfoI TCCNGGA 1 cut(s) 825
PkrI GCNGC 3 cut(s) 419, 430, 712
PleI GAGTC 2 cut(s) 66, 345
PpsI GAGTC 2 cut(s) 66, 345
PshBI ATTAAT 1 cut(s) 666
Psp6I CCWGG 2 cut(s) 714, 869
PspGI CCWGG 2 cut(s) 714, 869
PspN4I GGNNCC 2 cut(s) 6, 566
PspPI GGNCC 1 cut(s) 718
PvuII CAGCTG 1 cut(s) 710
RsaI GTAC 4 cut(s) 120, 620, 935, 1022
RsaNI GTAC 4 cut(s) 119, 619, 934, 1021
SaqAI TTAA 4 cut(s) 41, 156, 666, 864
SatI GCNGC 3 cut(s) 418, 429, 711
Sau3AI GATC 5 cut(s) 112, 252, 304, 540, 822
Sau96I GGNCC 1 cut(s) 718
ScaI AGTACT 1 cut(s) 120
SchI GAGTC 2 cut(s) 67, 346
ScrFI CCNGG 4 cut(s) 374, 716, 827, 871
SduI GDGCHC 2 cut(s) 374, 503
SfaNI GCATC 5 cut(s) 19, 411, 847, 1018, 1069
SmlI CTYRAG 1 cut(s) 863
SmoI CTYRAG 1 cut(s) 863
Sse9I AATT 4 cut(s) 20, 126, 992, 1088
SseBI AGGCCT 3 cut(s) 437, 780, 869
SsiI CCGC 2 cut(s) 418, 893
SspMI CTAG 4 cut(s) 149, 236, 422, 782
StuI AGGCCT 3 cut(s) 437, 780, 869
StyD4I CCNGG 4 cut(s) 372, 714, 825, 869
TaaI ACNGT 1 cut(s) 693
TaiI ACGT 1 cut(s) 1064
TaqI TCGA 3 cut(s) 251, 405, 1086
TasI AATT 4 cut(s) 20, 126, 992, 1088
TatI WGTACW 3 cut(s) 118, 618, 1020
TauI GCSGC 1 cut(s) 420
TfiI GAWTC 2 cut(s) 248, 787
Tru1I TTAA 4 cut(s) 41, 156, 666, 864
Tru9I TTAA 4 cut(s) 41, 156, 666, 864
TscAI CASTG 1 cut(s) 604
TseFI GTSAC 2 cut(s) 280, 895
TseI GCWGC 2 cut(s) 428, 710
Tsp45I GTSAC 2 cut(s) 280, 895
TspDTI ATGAA 6 cut(s) 275, 305, 503, 601, 629, 878
TspRI CASTG 1 cut(s) 604
Van91I CCANNNNNTGG 1 cut(s) 534
Vha464I CTTAAG 1 cut(s) 863
VspI ATTAAT 1 cut(s) 666
XapI RAATTY 1 cut(s) 992
XceI RCATGY 1 cut(s) 210
XspI CTAG 4 cut(s) 149, 236, 422, 782
ZrmI AGTACT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.