MD08G1138800.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000834
Physical Location & Seq
Reverse (-)
13324498 .. 13327097
2600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1138800.v1.1.491

Sequence Viewer

Length: 996 bp
ATGGATGATCAAGTACTCCCAATTCTTGATTTTTCAGATGGTTTAGTGTTAAAGCAAGGGAGTGAGGAATGGAAAGCTATGGGTAACAAAGTGAGAGAGGTATGTGAGGATTATGGTTGCTTTCTTTTGCTAGTGAAGGATGAGATCCCAATGAGTTTACGTGAAGAAATGGTGGCGGCAATGAAAGCTTTGTTTGATCTGCCTGAAGAGACCAAGAAGAAGCACACAAGTCCGAAGCCTTACCAGAGCTACGAAGGCAAGAGCCCTGTCATTCCCTTGAACGAAAGCTTCGGCATTGACGACGCTTGCTGCCTTGACGCAGCGCAGGCCTTCACCAACCTCATGTGGCCTCAAGGAAACCCTGCACTTTGTGAGGCAATGAAATCCATGTGCTCCAAGATGATGGAATTGAATCTCATCATCCTTAGGATGATCTTAGAGAGCTTTGGTTTAGGAAACCACTATGACTCCCATGTCCAAAATACCACCAGTGCTTTTCGTTTCATGAAGTACAAAGCTCCTCCTCCTCCTCCAACTCCAGGCAACATCAACATTAATGCTGACTCGTCATCAACATCACTGTCGGCGTCGCCATCAGTTGCCCTGGGCCTTGTGCCTCACACTGACAAAAGCACCCTAACCATTCTGTGCCAAAACGATGTGCAAGGCCTAGAGATTCTGACCAAACAAGGCAAATGGGTTGAAGTGATGGTCCCGGAGGGAGCTGTTGTGGTCTTTGTTGGGGATGCAATTAAGGCTTGGAGCAATGGGAGGCTTCATGCAGTGACGCACAGGGTTATGATAAGTGGGGACAAAGAGAGGTACTCGTATGCCCTATTCTCTTTGCCAAAAGAGGAGGTGGTGATTGAAGTGCCTCAAGAATTTATAGACAAAGAGCATCCTTTGCTCTACAAGCCATTCAACTTTGGTGCATACTTTACATACTTCACGTCCAATATTCGTGAGGATGCACTCGAATTATATGCTAGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

332

Amino Acids

37.0

Weight (kDa)

5.12

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 6 - 86 1.8e-13 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 197 - 281 7.4e-22 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 473
AciI CCGC 1 cut(s) 176
AclWI GGATC 1 cut(s) 139
AcsI RAATTY 1 cut(s) 881
AcuI CTGAAG 1 cut(s) 225
AcyI GRCGYC 1 cut(s) 587
AdeI CACNNNGTG 1 cut(s) 371
AfaI GTAC 3 cut(s) 15, 512, 824
AfiI CCNNNNNNNGG 1 cut(s) 539
AgsI TTSAA 5 cut(s) 280, 412, 704, 869, 922
AjiI CACGTC 1 cut(s) 951
AjnI CCWGG 2 cut(s) 538, 603
AluBI AGCT 7 cut(s) 77, 188, 249, 288, 444, 518, 725
AluI AGCT 7 cut(s) 77, 188, 249, 288, 444, 518, 725
Alw21I GWGCWC 1 cut(s) 395
Alw26I GTCTC 1 cut(s) 203
AlwI GGATC 1 cut(s) 139
AoxI GGCC 4 cut(s) 327, 347, 607, 667
ApeKI GCWGC 2 cut(s) 309, 320
ApoI RAATTY 1 cut(s) 881
AseI ATTAAT 1 cut(s) 555
AspLEI GCGC 1 cut(s) 325
AspS9I GGNCC 2 cut(s) 607, 712
AsuC2I CCSGG 1 cut(s) 716
AsuHPI GGTGA 2 cut(s) 325, 874
AvaII GGWCC 1 cut(s) 712
AxyI CCTNAGG 1 cut(s) 425
BanII GRGCYC 1 cut(s) 266
Bbv12I GWGCWC 1 cut(s) 395
BbvI GCAGC 2 cut(s) 296, 332
BccI CCATC 4 cut(s) 32, 397, 601, 703
BcgI CGANNNNNNTGC 1 cut(s) 965
BciT130I CCWGG 2 cut(s) 540, 605
BclI TGATCA 1 cut(s) 7
BcnI CCSGG 1 cut(s) 716
BcoDI GTCTC 1 cut(s) 203
BfaI CTAG 3 cut(s) 131, 671, 987
BisI GCNGC 3 cut(s) 177, 310, 321
BlsI GCNGC 3 cut(s) 178, 311, 322
BmcAI AGTACT 1 cut(s) 15
Bme1390I CCNGG 3 cut(s) 540, 605, 716
Bme18I GGWCC 1 cut(s) 712
BmgBI CACGTC 1 cut(s) 951
BmgT120I GGNCC 2 cut(s) 607, 712
BmiI GGNNCC 1 cut(s) 714
BmrFI CCNGG 3 cut(s) 540, 605, 716
BmsI GCATC 3 cut(s) 736, 907, 958
BplI GAGNNNNNCTC 2 cut(s) 809, 841
BpmI CTGGAG 1 cut(s) 522
BpuEI CTTGAG 2 cut(s) 336, 861
BpuMI CCSGG 1 cut(s) 716
BsaAI YACGTR 1 cut(s) 161
BsaHI GRCGYC 1 cut(s) 587
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 2 cut(s) 603, 604
BsaXI ACNNNNNCTCC 4 cut(s) 452, 482, 714, 744
Bsc4I CCNNNNNNNGG 1 cut(s) 539
Bse1I ACTGG 1 cut(s) 489
Bse21I CCTNAGG 1 cut(s) 425
Bse3DI GCAATG 3 cut(s) 186, 384, 772
BseBI CCWGG 2 cut(s) 540, 605
BseDI CCNNGG 2 cut(s) 603, 604
BseGI GGATG 7 cut(s) 10, 145, 420, 435, 751, 898, 973
BseLI CCNNNNNNNGG 1 cut(s) 539
BseMI GCAATG 3 cut(s) 186, 384, 772
BseNI ACTGG 1 cut(s) 489
BseRI GAGGAG 5 cut(s) 510, 513, 516, 519, 869
BseXI GCAGC 2 cut(s) 296, 332
BsgI GTGCAG 1 cut(s) 348
BshFI GGCC 4 cut(s) 329, 349, 609, 669
BsiHKAI GWGCWC 1 cut(s) 395
BsiSI CCGG 1 cut(s) 716
BslFI GGGAC 2 cut(s) 698, 824
BslI CCNNNNNNNGG 1 cut(s) 539
BsmAI GTCTC 1 cut(s) 203
BsmFI GGGAC 2 cut(s) 698, 824
BsnI GGCC 4 cut(s) 329, 349, 609, 669
Bso31I GGTCTC 1 cut(s) 203
Bsp1286I GDGCHC 2 cut(s) 266, 395
Bsp143I GATC 4 cut(s) 7, 144, 196, 432
BspACI CCGC 1 cut(s) 176
BspANI GGCC 4 cut(s) 329, 349, 609, 669
BspHI TCATGA 1 cut(s) 504
BspLI GGNNCC 1 cut(s) 714
BspPI GGATC 1 cut(s) 139
BspTNI GGTCTC 1 cut(s) 203
BsrDI GCAATG 3 cut(s) 186, 384, 772
BsrI ACTGG 1 cut(s) 489
BssECI CCNNGG 2 cut(s) 603, 604
BssMI GATC 4 cut(s) 7, 144, 196, 432
BssNI GRCGYC 1 cut(s) 587
Bst2UI CCWGG 2 cut(s) 540, 605
Bst4CI ACNGT 1 cut(s) 582
Bst6I CTCTTC 1 cut(s) 201
BstACI GRCGYC 1 cut(s) 587
BstAPI GCANNNNNTGC 1 cut(s) 904
BstBAI YACGTR 1 cut(s) 161
BstC8I GCNNGC 2 cut(s) 307, 327
BstDEI CTNAG 2 cut(s) 425, 436
BstF5I GGATG 7 cut(s) 10, 145, 420, 435, 751, 898, 973
BstHHI GCGC 1 cut(s) 325
BstKTI GATC 4 cut(s) 10, 147, 199, 435
BstMAI GTCTC 1 cut(s) 203
BstMBI GATC 4 cut(s) 7, 144, 196, 432
BstMWI GCNNNNNNNGC 6 cut(s) 185, 255, 326, 755, 904, 913
BstNI CCWGG 2 cut(s) 540, 605
BstSCI CCNGG 3 cut(s) 538, 603, 714
BstV1I GCAGC 2 cut(s) 296, 332
BstX2I RGATCY 1 cut(s) 144
BstXI CCANNNNNNTGG 1 cut(s) 403
BstYI RGATCY 1 cut(s) 144
Bsu36I CCTNAGG 1 cut(s) 425
BsuRI GGCC 4 cut(s) 329, 349, 609, 669
BtrI CACGTC 1 cut(s) 951
BtsCI GGATG 7 cut(s) 10, 145, 420, 435, 751, 898, 973
BtsI GCAGTG 1 cut(s) 789
BtsIMutI CAGTG 4 cut(s) 496, 578, 621, 789
Cac8I GCNNGC 2 cut(s) 307, 327
CciI TCATGA 1 cut(s) 504
CfoI GCGC 1 cut(s) 325
Cfr13I GGNCC 2 cut(s) 607, 712
CseI GACGC 4 cut(s) 311, 326, 576, 796
Csp6I GTAC 3 cut(s) 14, 511, 823
CviAII CATG 5 cut(s) 343, 388, 473, 505, 779
CviQI GTAC 3 cut(s) 14, 511, 823
DdeI CTNAG 2 cut(s) 425, 436
DpnI GATC 4 cut(s) 9, 146, 198, 434
DpnII GATC 4 cut(s) 7, 144, 196, 432
DraIII CACNNNGTG 1 cut(s) 371
DrdI GACNNNNNNGTC 1 cut(s) 473
DseDI GACNNNNNNGTC 1 cut(s) 473
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Eco147I AGGCCT 2 cut(s) 329, 669
Eco24I GRGCYC 1 cut(s) 266
Eco31I GGTCTC 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 712
Eco57I CTGAAG 1 cut(s) 225
Eco81I CCTNAGG 1 cut(s) 425
EcoRII CCWGG 2 cut(s) 538, 603
EcoT38I GRGCYC 1 cut(s) 266
FaeI CATG 5 cut(s) 346, 391, 476, 508, 782
FaqI GGGAC 2 cut(s) 698, 824
FatI CATG 5 cut(s) 342, 387, 472, 504, 778
FbaI TGATCA 1 cut(s) 7
Fnu4HI GCNGC 3 cut(s) 177, 310, 321
FokI GGATG 7 cut(s) 17, 152, 407, 442, 758, 885, 980
FriOI GRGCYC 1 cut(s) 266
Fsp4HI GCNGC 3 cut(s) 177, 310, 321
FspBI CTAG 3 cut(s) 131, 671, 987
GlaI GCGC 1 cut(s) 324
GluI GCNGC 3 cut(s) 177, 310, 321
GsuI CTGGAG 1 cut(s) 522
HaeIII GGCC 4 cut(s) 329, 349, 609, 669
HapII CCGG 1 cut(s) 716
HgaI GACGC 4 cut(s) 311, 326, 576, 796
HhaI GCGC 1 cut(s) 325
Hin1I GRCGYC 1 cut(s) 587
Hin1II CATG 5 cut(s) 346, 391, 476, 508, 782
Hin6I GCGC 1 cut(s) 323
HinP1I GCGC 1 cut(s) 323
HindIII AAGCTT 2 cut(s) 186, 286
HinfI GANTC 4 cut(s) 412, 467, 563, 676
HpaII CCGG 1 cut(s) 716
HphI GGTGA 2 cut(s) 325, 874
Hpy166II GTNNAC 1 cut(s) 158
Hpy188I TCNGA 3 cut(s) 37, 234, 681
Hpy188III TCNNGA 4 cut(s) 26, 505, 878, 962
Hpy8I GTNNAC 1 cut(s) 158
Hpy99I CGWCG 2 cut(s) 305, 592
HpyAV CCTTC 3 cut(s) 130, 248, 340
HpyCH4III ACNGT 1 cut(s) 582
HpyCH4IV ACGT 2 cut(s) 160, 950
HpyCH4V TGCA 6 cut(s) 365, 664, 749, 782, 932, 971
HpyF10VI GCNNNNNNNGC 6 cut(s) 185, 255, 326, 755, 904, 913
HpyF3I CTNAG 2 cut(s) 425, 436
HpySE526I ACGT 2 cut(s) 160, 950
Hsp92I GRCGYC 1 cut(s) 587
Hsp92II CATG 5 cut(s) 346, 391, 476, 508, 782
HspAI GCGC 1 cut(s) 323
Ksp22I TGATCA 1 cut(s) 7
Kzo9I GATC 4 cut(s) 7, 144, 196, 432
LmnI GCTCC 4 cut(s) 398, 523, 722, 762
Lsp1109I GCAGC 2 cut(s) 296, 332
LweI GCATC 3 cut(s) 736, 907, 958
MaeI CTAG 3 cut(s) 131, 671, 987
MaeII ACGT 2 cut(s) 160, 950
MaeIII GTNAC 2 cut(s) 83, 784
MalI GATC 4 cut(s) 9, 146, 198, 434
MboI GATC 4 cut(s) 7, 144, 196, 432
MboII GAAGA 3 cut(s) 176, 218, 229
MflI RGATCY 1 cut(s) 144
MhlI GDGCHC 2 cut(s) 266, 395
MluCI AATT 5 cut(s) 21, 407, 750, 881, 977
MlyI GAGTC 2 cut(s) 461, 557
MmeI TCCRAC 1 cut(s) 557
MseI TTAA 3 cut(s) 50, 555, 753
MspI CCGG 1 cut(s) 716
MspR9I CCNGG 3 cut(s) 540, 605, 716
MvaI CCWGG 2 cut(s) 540, 605
MwoI GCNNNNNNNGC 6 cut(s) 185, 255, 326, 755, 904, 913
NciI CCSGG 1 cut(s) 716
NdeII GATC 4 cut(s) 7, 144, 196, 432
NlaIII CATG 5 cut(s) 346, 391, 476, 508, 782
NlaIV GGNNCC 1 cut(s) 714
NmuCI GTSAC 1 cut(s) 784
PagI TCATGA 1 cut(s) 504
PasI CCCWGGG 1 cut(s) 604
PceI AGGCCT 2 cut(s) 329, 669
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PfeI GAWTC 2 cut(s) 412, 676
PfoI TCCNGGA 1 cut(s) 714
PkrI GCNGC 3 cut(s) 178, 311, 322
PleI GAGTC 2 cut(s) 461, 557
PpsI GAGTC 2 cut(s) 461, 557
Ppu21I YACGTR 1 cut(s) 161
PshBI ATTAAT 1 cut(s) 555
Psp6I CCWGG 2 cut(s) 538, 603
PspGI CCWGG 2 cut(s) 538, 603
PspN4I GGNNCC 1 cut(s) 714
PspPI GGNCC 2 cut(s) 607, 712
PsuI RGATCY 1 cut(s) 144
RsaI GTAC 3 cut(s) 15, 512, 824
RsaNI GTAC 3 cut(s) 14, 511, 823
SaqAI TTAA 3 cut(s) 50, 555, 753
SatI GCNGC 3 cut(s) 177, 310, 321
Sau3AI GATC 4 cut(s) 7, 144, 196, 432
Sau96I GGNCC 2 cut(s) 607, 712
ScaI AGTACT 1 cut(s) 15
SchI GAGTC 2 cut(s) 461, 557
ScrFI CCNGG 3 cut(s) 540, 605, 716
SduI GDGCHC 2 cut(s) 266, 395
SfaNI GCATC 3 cut(s) 736, 907, 958
SinI GGWCC 1 cut(s) 712
SmlI CTYRAG 2 cut(s) 351, 876
SmoI CTYRAG 2 cut(s) 351, 876
Sse9I AATT 5 cut(s) 21, 407, 750, 881, 977
SseBI AGGCCT 2 cut(s) 329, 669
SsiI CCGC 1 cut(s) 176
SspI AATATT 1 cut(s) 958
SspMI CTAG 3 cut(s) 131, 671, 987
StuI AGGCCT 2 cut(s) 329, 669
StyD4I CCNGG 3 cut(s) 538, 603, 714
TaaI ACNGT 1 cut(s) 582
TaiI ACGT 2 cut(s) 163, 953
TaqI TCGA 1 cut(s) 975
TasI AATT 5 cut(s) 21, 407, 750, 881, 977
TatI WGTACW 2 cut(s) 13, 510
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 2 cut(s) 412, 676
Tru1I TTAA 3 cut(s) 50, 555, 753
Tru9I TTAA 3 cut(s) 50, 555, 753
TscAI CASTG 4 cut(s) 496, 585, 628, 789
TseFI GTSAC 1 cut(s) 784
TseI GCWGC 2 cut(s) 309, 320
Tsp45I GTSAC 1 cut(s) 784
TspDTI ATGAA 5 cut(s) 197, 395, 493, 521, 767
TspRI CASTG 4 cut(s) 496, 585, 628, 789
VpaK11BI GGWCC 1 cut(s) 712
VspI ATTAAT 1 cut(s) 555
XapI RAATTY 1 cut(s) 881
XspI CTAG 3 cut(s) 131, 671, 987
ZrmI AGTACT 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.