FvH4_2g29991

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000834
Physical Location & Seq
Forward (+)
23254982 .. 23257700
2719 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29991.t1

Sequence Viewer

Length: 981 bp
ATGGGTGAGGAGGTTGATCATGATCAAGCACTACTAGTACCAGTTCTTGAATTTTCGAAGGAGTGTTTGGTCCCAAAGCAAGAGAGGAGTGAGGAATGGGGAGCTATGAGCACCAAAGTGAGAGAGGCATGTGAGAGTCATGGCTGCTTTCTGCTGTTGGTGCAGGAAGAGGTGATGCCGATGCGCTTACGTGAAGACATGGTGATGGCCATGAAAGCTTTGTTTGATCTCCCTGAAGAGACCAAGCAGAAGCACACAAGCCCCAGGGCTTACCAGAGCTACCAAGGCAAGTGCCCCATCATTCCCTTGAACGAAAGCTTCGGGATCGACAATGCATGCCAGCCTGATGCAGCCGAGTCCTTCACCAACCTCATGTGGCTTCAAGGGAACTCTTCTTTTTGTGAGACAATGAAATCCATGAGCTCAAAGATGATGGAGTTGAACTTCACCGTCCTCGAAATGGTTTTGGAGAGTTTAGGCGTGGGAAAGCACTACACCTCGCACGTAGAGAACACCACCGCCGTGATGCGTTTCATGAAGTACAAAATCCCTCCGATCAGCAACGACGTCAGGGACACATCCGTTGGTTTGGTGCCTCACACAGACAAGAGTAGCTTAACAATTCTATGCCAGAACGAAGTGCAGGGTCTGGAGATCCTCACCAAACAAGGCCAATGGGTTCAAGTCCCGGTCCCCGAAAGAGCCGTCGTGGTCTTCGTTGGGGATGCACTCAAGGCTTGGAGCAACGGGAGGCTTCATGCAGTGACTCACAGGGTGATGATGAGTAAAGACATTAAGGAAGAAAGGTACTCTTTTGCGATGTTTGGGTTGCCGAAGGAGGAGTTCATGATCGAAGTGCCGGATGAGCTTATCGACGATGAGCATCCTCTGCTGTACCGGCCGTTCAACTTTGCCGAGTACTACAACTATTTTACGGCCAATGTTCGTGACGATGCACTGGAAGTCTATGCTGGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

327

Amino Acids

37.2

Weight (kDa)

4.95

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 13 - 96 1.3e-10 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 180 - 275 2.1e-21 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 570
AccB1I GGYRCC 1 cut(s) 592
AciI CCGC 1 cut(s) 519
AclWI GGATC 2 cut(s) 332, 649
AcoI YGGCCR 3 cut(s) 207, 899, 936
AcsI RAATTY 1 cut(s) 50
AcuI CTGAAG 1 cut(s) 255
AcyI GRCGYC 1 cut(s) 567
AdeI CACNNNGTG 1 cut(s) 775
AfaI GTAC 5 cut(s) 39, 542, 809, 896, 920
AfiI CCNNNNNNNGG 1 cut(s) 460
AgsI TTSAA 6 cut(s) 50, 310, 383, 442, 683, 907
AhlI ACTAGT 1 cut(s) 34
AjnI CCWGG 1 cut(s) 263
AjuI GAANNNNNNNTTGG 2 cut(s) 50, 82
AleI CACNNNNGTG 2 cut(s) 116, 521
AluBI AGCT 7 cut(s) 104, 218, 279, 318, 423, 615, 868
AluI AGCT 7 cut(s) 104, 218, 279, 318, 423, 615, 868
Alw21I GWGCWC 2 cut(s) 113, 425
Alw26I GTCTC 2 cut(s) 233, 398
AlwI GGATC 2 cut(s) 332, 649
AlwNI CAGNNNCTG 1 cut(s) 649
AoxI GGCC 4 cut(s) 207, 670, 899, 936
ApeKI GCWGC 2 cut(s) 144, 350
ApoI RAATTY 1 cut(s) 50
ArsI GACNNNNNNTTYG 2 cut(s) 690, 722
AspLEI GCGC 1 cut(s) 186
AspS9I GGNCC 2 cut(s) 70, 691
AsuC2I CCSGG 1 cut(s) 689
AsuHPI GGTGA 7 cut(s) 17, 184, 214, 355, 439, 652, 787
AsuII TTCGAA 1 cut(s) 56
AvaII GGWCC 2 cut(s) 70, 691
BaeGI GKGCMC 1 cut(s) 296
BalI TGGCCA 1 cut(s) 209
BanI GGYRCC 1 cut(s) 592
BanII GRGCYC 1 cut(s) 425
BbsI GAAGAC 2 cut(s) 201, 706
Bbv12I GWGCWC 2 cut(s) 113, 425
BbvI GCAGC 2 cut(s) 131, 362
BccI CCATC 3 cut(s) 199, 305, 427
BceAI ACGGC 4 cut(s) 506, 689, 886, 951
BciT130I CCWGG 1 cut(s) 265
BclI TGATCA 2 cut(s) 16, 22
BcnI CCSGG 1 cut(s) 689
BcoDI GTCTC 2 cut(s) 233, 398
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 1 cut(s) 35
BisI GCNGC 2 cut(s) 145, 351
BlsI GCNGC 2 cut(s) 146, 352
BmcAI AGTACT 1 cut(s) 920
Bme1390I CCNGG 2 cut(s) 265, 689
Bme18I GGWCC 2 cut(s) 70, 691
BmgT120I GGNCC 2 cut(s) 70, 691
BmiI GGNNCC 3 cut(s) 72, 594, 693
BmrFI CCNGG 2 cut(s) 265, 689
BmsI GCATC 7 cut(s) 165, 171, 337, 516, 715, 892, 943
BpiI GAAGAC 2 cut(s) 201, 706
BpmI CTGGAG 1 cut(s) 671
Bpu14I TTCGAA 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 716
BpuMI CCSGG 1 cut(s) 689
BsaAI YACGTR 2 cut(s) 191, 505
BsaBI GATNNNNATC 2 cut(s) 21, 882
BsaHI GRCGYC 1 cut(s) 567
BsaI GGTCTC 1 cut(s) 233
BsaJI CCNNGG 3 cut(s) 263, 264, 283
Bsc4I CCNNNNNNNGG 1 cut(s) 460
Bse118I RCCGGY 1 cut(s) 897
Bse1I ACTGG 2 cut(s) 41, 963
Bse8I GATNNNNATC 2 cut(s) 21, 882
BseBI CCWGG 1 cut(s) 265
BseDI CCNNGG 3 cut(s) 263, 264, 283
BseGI GGATG 4 cut(s) 578, 730, 868, 883
BseJI GATNNNNATC 2 cut(s) 21, 882
BseLI CCNNNNNNNGG 1 cut(s) 460
BseNI ACTGG 2 cut(s) 41, 963
BseRI GAGGAG 3 cut(s) 23, 100, 854
BseSI GKGCMC 1 cut(s) 296
BseX3I CGGCCG 1 cut(s) 899
BseXI GCAGC 2 cut(s) 131, 362
BsgI GTGCAG 2 cut(s) 182, 662
Bsh1285I CGRYCG 1 cut(s) 902
BshFI GGCC 4 cut(s) 209, 672, 901, 938
BshNI GGYRCC 1 cut(s) 592
BsiEI CGRYCG 1 cut(s) 902
BsiHKAI GWGCWC 2 cut(s) 113, 425
BsiSI CCGG 3 cut(s) 689, 860, 898
BslFI GGGAC 4 cut(s) 56, 587, 671, 677
BslI CCNNNNNNNGG 1 cut(s) 460
BsmAI GTCTC 2 cut(s) 233, 398
BsmFI GGGAC 4 cut(s) 56, 587, 671, 677
BsnI GGCC 4 cut(s) 209, 672, 901, 938
Bso31I GGTCTC 1 cut(s) 233
Bsp119I TTCGAA 1 cut(s) 56
Bsp1286I GDGCHC 3 cut(s) 113, 296, 425
Bsp143I GATC 7 cut(s) 16, 22, 226, 324, 555, 654, 849
BspACI CCGC 1 cut(s) 519
BspANI GGCC 4 cut(s) 209, 672, 901, 938
BspHI TCATGA 3 cut(s) 19, 534, 846
BspLI GGNNCC 3 cut(s) 72, 594, 693
BspPI GGATC 2 cut(s) 332, 649
BspT104I TTCGAA 1 cut(s) 56
BspT107I GGYRCC 1 cut(s) 592
BspTNI GGTCTC 1 cut(s) 233
BsrFI RCCGGY 1 cut(s) 897
BsrI ACTGG 2 cut(s) 41, 963
BssAI RCCGGY 1 cut(s) 897
BssECI CCNNGG 3 cut(s) 263, 264, 283
BssMI GATC 7 cut(s) 16, 22, 226, 324, 555, 654, 849
BssNI GRCGYC 1 cut(s) 567
BssT1I CCWWGG 1 cut(s) 283
Bst2UI CCWGG 1 cut(s) 265
Bst4CI ACNGT 1 cut(s) 451
Bst6I CTCTTC 3 cut(s) 162, 231, 397
BstACI GRCGYC 1 cut(s) 567
BstAPI GCANNNNNTGC 1 cut(s) 889
BstBAI YACGTR 2 cut(s) 191, 505
BstBI TTCGAA 1 cut(s) 56
BstC8I GCNNGC 2 cut(s) 337, 341
BstF5I GGATG 4 cut(s) 578, 730, 868, 883
BstHHI GCGC 1 cut(s) 186
BstKTI GATC 7 cut(s) 19, 25, 229, 327, 558, 657, 852
BstMAI GTCTC 2 cut(s) 233, 398
BstMBI GATC 7 cut(s) 16, 22, 226, 324, 555, 654, 849
BstMCI CGRYCG 1 cut(s) 902
BstMWI GCNNNNNNNGC 7 cut(s) 160, 215, 285, 734, 865, 889, 898
BstNI CCWGG 1 cut(s) 265
BstNSI RCATGY 2 cut(s) 132, 339
BstSCI CCNGG 2 cut(s) 263, 687
BstSLI GKGCMC 1 cut(s) 296
BstV1I GCAGC 2 cut(s) 131, 362
BstV2I GAAGAC 2 cut(s) 201, 706
BstX2I RGATCY 1 cut(s) 654
BstYI RGATCY 1 cut(s) 654
BstZI CGGCCG 1 cut(s) 899
BsuRI GGCC 4 cut(s) 209, 672, 901, 938
BtgZI GCGATG 1 cut(s) 833
BtsCI GGATG 4 cut(s) 578, 730, 868, 883
BtsI GCAGTG 1 cut(s) 768
BtsIMutI CAGTG 2 cut(s) 768, 956
Cac8I GCNNGC 2 cut(s) 337, 341
CaiI CAGNNNCTG 1 cut(s) 649
CciI TCATGA 3 cut(s) 19, 534, 846
CfoI GCGC 1 cut(s) 186
Cfr10I RCCGGY 1 cut(s) 897
Cfr13I GGNCC 2 cut(s) 70, 691
Csp6I GTAC 5 cut(s) 38, 541, 808, 895, 919
CviQI GTAC 5 cut(s) 38, 541, 808, 895, 919
DpnI GATC 7 cut(s) 18, 24, 228, 326, 557, 656, 851
DpnII GATC 7 cut(s) 16, 22, 226, 324, 555, 654, 849
DraIII CACNNNGTG 1 cut(s) 775
EaeI YGGCCR 3 cut(s) 207, 899, 936
EagI CGGCCG 1 cut(s) 899
Eam1104I CTCTTC 3 cut(s) 162, 231, 397
EarI CTCTTC 3 cut(s) 162, 231, 397
Ecl136II GAGCTC 1 cut(s) 423
EclXI CGGCCG 1 cut(s) 899
Eco130I CCWWGG 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 425
Eco31I GGTCTC 1 cut(s) 233
Eco47I GGWCC 2 cut(s) 70, 691
Eco52I CGGCCG 1 cut(s) 899
Eco53kI GAGCTC 1 cut(s) 423
Eco57I CTGAAG 1 cut(s) 255
EcoICRI GAGCTC 1 cut(s) 423
EcoRII CCWGG 1 cut(s) 263
EcoT14I CCWWGG 1 cut(s) 283
EcoT22I ATGCAT 1 cut(s) 337
EcoT38I GRGCYC 1 cut(s) 425
ErhI CCWWGG 1 cut(s) 283
FalI AAGNNNNNCTT 4 cut(s) 599, 631, 796, 828
FaqI GGGAC 4 cut(s) 56, 587, 671, 677
FbaI TGATCA 2 cut(s) 16, 22
Fnu4HI GCNGC 2 cut(s) 145, 351
FokI GGATG 4 cut(s) 565, 737, 870, 875
FriOI GRGCYC 1 cut(s) 425
Fsp4HI GCNGC 2 cut(s) 145, 351
FspBI CTAG 1 cut(s) 35
GlaI GCGC 1 cut(s) 185
GluI GCNGC 2 cut(s) 145, 351
GsuI CTGGAG 1 cut(s) 671
HaeIII GGCC 4 cut(s) 209, 672, 901, 938
HapII CCGG 3 cut(s) 689, 860, 898
HhaI GCGC 1 cut(s) 186
Hin1I GRCGYC 1 cut(s) 567
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HindIII AAGCTT 2 cut(s) 216, 316
HinfI GANTC 3 cut(s) 136, 356, 766
HpaII CCGG 3 cut(s) 689, 860, 898
HphI GGTGA 7 cut(s) 17, 184, 214, 355, 439, 652, 787
Hpy188I TCNGA 1 cut(s) 555
Hpy188III TCNNGA 7 cut(s) 20, 47, 322, 535, 650, 847, 947
Hpy99I CGWCG 3 cut(s) 569, 710, 878
HpyAV CCTTC 3 cut(s) 52, 370, 829
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4IV ACGT 3 cut(s) 190, 504, 567
HpyCH4V TGCA 7 cut(s) 163, 335, 350, 643, 728, 761, 956
HpyF10VI GCNNNNNNNGC 7 cut(s) 160, 215, 285, 734, 865, 889, 898
HpySE526I ACGT 3 cut(s) 190, 504, 567
Hsp92I GRCGYC 1 cut(s) 567
HspAI GCGC 1 cut(s) 184
Ksp22I TGATCA 2 cut(s) 16, 22
Kzo9I GATC 7 cut(s) 16, 22, 226, 324, 555, 654, 849
LmnI GCTCC 2 cut(s) 101, 741
Lsp1109I GCAGC 2 cut(s) 131, 362
LweI GCATC 7 cut(s) 165, 171, 337, 516, 715, 892, 943
MaeI CTAG 1 cut(s) 35
MaeII ACGT 3 cut(s) 190, 504, 567
MaeIII GTNAC 2 cut(s) 763, 947
MalI GATC 7 cut(s) 18, 24, 228, 326, 557, 656, 851
MboI GATC 7 cut(s) 16, 22, 226, 324, 555, 654, 849
MboII GAAGA 6 cut(s) 179, 206, 248, 384, 706, 812
MflI RGATCY 1 cut(s) 654
MhlI GDGCHC 3 cut(s) 113, 296, 425
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 2 cut(s) 50, 621
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 3 cut(s) 145, 365, 760
Mox20I TGGCCA 1 cut(s) 209
Mph1103I ATGCAT 1 cut(s) 337
MscI TGGCCA 1 cut(s) 209
MseI TTAA 2 cut(s) 617, 795
MslI CAYNNNNRTG 3 cut(s) 116, 203, 521
Msp20I TGGCCA 1 cut(s) 209
MspI CCGG 3 cut(s) 689, 860, 898
MspR9I CCNGG 2 cut(s) 265, 689
MvaI CCWGG 1 cut(s) 265
MwoI GCNNNNNNNGC 7 cut(s) 160, 215, 285, 734, 865, 889, 898
NciI CCSGG 1 cut(s) 689
NdeII GATC 7 cut(s) 16, 22, 226, 324, 555, 654, 849
NlaIV GGNNCC 3 cut(s) 72, 594, 693
NmeAIII GCCGAG 2 cut(s) 379, 940
NmuCI GTSAC 2 cut(s) 763, 947
NsiI ATGCAT 1 cut(s) 337
NspI RCATGY 2 cut(s) 132, 339
NspV TTCGAA 1 cut(s) 56
OliI CACNNNNGTG 2 cut(s) 116, 521
PaeI GCATGC 1 cut(s) 339
PagI TCATGA 3 cut(s) 19, 534, 846
PasI CCCWGGG 1 cut(s) 264
PcsI WCGNNNNNNNCGW 1 cut(s) 714
PkrI GCNGC 2 cut(s) 146, 352
PleI GAGTC 3 cut(s) 144, 364, 760
PpsI GAGTC 3 cut(s) 144, 364, 760
Ppu21I YACGTR 2 cut(s) 191, 505
Psp124BI GAGCTC 1 cut(s) 425
Psp6I CCWGG 1 cut(s) 263
PspGI CCWGG 1 cut(s) 263
PspN4I GGNNCC 3 cut(s) 72, 594, 693
PspPI GGNCC 2 cut(s) 70, 691
PsrI GAACNNNNNNTAC 2 cut(s) 887, 919
PstNI CAGNNNCTG 1 cut(s) 649
PsuI RGATCY 1 cut(s) 654
RsaI GTAC 5 cut(s) 39, 542, 809, 896, 920
RsaNI GTAC 5 cut(s) 38, 541, 808, 895, 919
RseI CAYNNNNRTG 3 cut(s) 116, 203, 521
SacI GAGCTC 1 cut(s) 425
SaqAI TTAA 2 cut(s) 617, 795
SatI GCNGC 2 cut(s) 145, 351
Sau3AI GATC 7 cut(s) 16, 22, 226, 324, 555, 654, 849
Sau96I GGNCC 2 cut(s) 70, 691
ScaI AGTACT 1 cut(s) 920
SchI GAGTC 3 cut(s) 145, 365, 760
ScrFI CCNGG 2 cut(s) 265, 689
SduI GDGCHC 3 cut(s) 113, 296, 425
SfaNI GCATC 7 cut(s) 165, 171, 337, 516, 715, 892, 943
SfuI TTCGAA 1 cut(s) 56
SinI GGWCC 2 cut(s) 70, 691
SmiMI CAYNNNNRTG 3 cut(s) 116, 203, 521
SmlI CTYRAG 1 cut(s) 731
SmoI CTYRAG 1 cut(s) 731
SpeI ACTAGT 1 cut(s) 34
SphI GCATGC 1 cut(s) 339
Sse9I AATT 2 cut(s) 50, 621
SsiI CCGC 1 cut(s) 519
SspMI CTAG 1 cut(s) 35
SstI GAGCTC 1 cut(s) 425
StyD4I CCNGG 2 cut(s) 263, 687
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 1 cut(s) 451
TaiI ACGT 3 cut(s) 193, 507, 570
TaqI TCGA 5 cut(s) 56, 327, 456, 852, 873
TasI AATT 2 cut(s) 50, 621
TatI WGTACW 2 cut(s) 540, 918
Tru1I TTAA 2 cut(s) 617, 795
Tru9I TTAA 2 cut(s) 617, 795
TscAI CASTG 2 cut(s) 768, 963
TseFI GTSAC 2 cut(s) 763, 947
TseI GCWGC 2 cut(s) 144, 350
Tsp45I GTSAC 2 cut(s) 763, 947
TspDTI ATGAA 6 cut(s) 227, 425, 523, 551, 746, 835
TspGWI ACGGA 1 cut(s) 571
TspRI CASTG 2 cut(s) 768, 963
VpaK11BI GGWCC 2 cut(s) 70, 691
XapI RAATTY 1 cut(s) 50
XceI RCATGY 2 cut(s) 132, 339
XspI CTAG 1 cut(s) 35
ZraI GACGTC 1 cut(s) 568
ZrmI AGTACT 1 cut(s) 920
Zsp2I ATGCAT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.