MD15G1116900.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000834
Physical Location & Seq
Reverse (-)
8282239 .. 8284926
2688 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1116900.v1.1.491

Sequence Viewer

Length: 1017 bp
ATGGAGATGGATGATCAAGTACTCCCAATTCTTGATTTTTCAGAGGGTCTAGTGTTTAAGCAAGGGAGCGAGGAGTGGAAAGCTATGAGTAACAAAGTGAGAGAGGCATGTGAGGATTATGGTTGCTTTCTTTTGCTAGTGAGGGATGAGATCCCCATGAAGTTGCGTGAAGAAATGGTGACGGCCATGAAAGCTTTGTTTGATCTGCCTGAAGAGACCAAGCATAAGCACAAGATTTTCAAGCCTTATCAGAGCTACGAAGGCAGGAGCCCGGTCATTCCCTTGAACGAAAGCTTCGGCATCGATGACGCTTGCCGCCTCGACGCAGCTCAGGCCTTCACCAACCTCATGTGGCCTCAAGGAAACCCTGCATTTTGTGAGGCAATGAAATCCATGTGCTCCAAGATGATGGACTTGAATATCACCATCCTTTGGATGATCTTAGAGAGCTTTGGTTTGGGGAACCACTATGACACTCATGTCCAAAACACCACCAGTGCTTTTCGTTTCATGAAGTACAAAGCTCCTCCTCCTCCTCCTCCAACTCCAAGCAACATCAATATTAATGTTGGCTCATCAACAGCATCACCGTCGGCGTCCCCATCAGCTGCCCTGGGCCTTGTGCCTCATACTGACAAAAATACCCTAACCATTCTGTGCCAAAACGATGTGCAAGGCCTAGAGATTCTGACCAAACAAGGCAAATGGGTTGAAGTGATGGTCCCAGAGGGAGCTGTTGTGGTCTTTGTTGGTGATGCACTTAAGGCCTGGAGCAATGGGAGGCTTCATGCAGTGATGCACAGGGTTATGATGAGTGGGGACAAAGAGAGGTACTCTTATTCCCTATTTTCTGTGCCAAAAGAGGAGGTGGTGATTGAAGTGCCACAGGAATTTATAGACAAGGAGCATCCTTTGTTGTACAAGCCATTCAACTTTGCCGAATACTTTACATACTTCACGTCCAATGTTCGTGAGGATGCACTCGAATTATATGCTGGAGTTGATATTATGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

339

Amino Acids

38.18

Weight (kDa)

5.07

Isoelectric Point (pI)

53.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 8 - 88 3.4e-13 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 197 - 284 9.5e-22 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 479
AccB7I CCANNNNNTGG 1 cut(s) 432
AciI CCGC 1 cut(s) 316
AclWI GGATC 1 cut(s) 145
AcoI YGGCCR 1 cut(s) 183
AcsI RAATTY 1 cut(s) 890
AcuI CTGAAG 1 cut(s) 231
AcyI GRCGYC 1 cut(s) 596
AfaI GTAC 4 cut(s) 21, 518, 833, 920
AfiI CCNNNNNNNGG 1 cut(s) 432
AflII CTTAAG 1 cut(s) 761
AgsI TTSAA 6 cut(s) 241, 286, 418, 713, 878, 931
AjiI CACGTC 1 cut(s) 960
AjnI CCWGG 2 cut(s) 612, 767
AluBI AGCT 9 cut(s) 83, 194, 255, 294, 329, 450, 524, 608, 734
AluI AGCT 9 cut(s) 83, 194, 255, 294, 329, 450, 524, 608, 734
Alw21I GWGCWC 1 cut(s) 401
Alw26I GTCTC 1 cut(s) 209
AlwI GGATC 1 cut(s) 145
AoxI GGCC 6 cut(s) 183, 333, 353, 616, 676, 765
ApeKI GCWGC 2 cut(s) 326, 608
ApoI RAATTY 1 cut(s) 890
AseI ATTAAT 1 cut(s) 564
AspS9I GGNCC 2 cut(s) 616, 721
AsuC2I CCSGG 1 cut(s) 272
AsuHPI GGTGA 6 cut(s) 190, 331, 415, 579, 764, 883
AvaII GGWCC 1 cut(s) 721
BanII GRGCYC 1 cut(s) 272
Bbv12I GWGCWC 1 cut(s) 401
BbvI GCAGC 2 cut(s) 338, 595
BccI CCATC 4 cut(s) 403, 434, 610, 712
BceAI ACGGC 1 cut(s) 198
BcgI CGANNNNNNTGC 4 cut(s) 573, 607, 974, 1008
BciT130I CCWGG 2 cut(s) 614, 769
BclI TGATCA 1 cut(s) 13
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 1 cut(s) 209
BfaI CTAG 3 cut(s) 50, 137, 680
BfrI CTTAAG 1 cut(s) 761
BisI GCNGC 3 cut(s) 316, 327, 609
BlsI GCNGC 3 cut(s) 317, 328, 610
BmcAI AGTACT 1 cut(s) 21
Bme1390I CCNGG 3 cut(s) 272, 614, 769
Bme18I GGWCC 1 cut(s) 721
BmgBI CACGTC 1 cut(s) 960
BmgT120I GGNCC 2 cut(s) 616, 721
BmiI GGNNCC 3 cut(s) 269, 464, 723
BmrFI CCNGG 3 cut(s) 272, 614, 769
BmsI GCATC 6 cut(s) 309, 593, 745, 786, 916, 967
BplI GAGNNNNNCTC 2 cut(s) 818, 850
BpmI CTGGAG 2 cut(s) 790, 1017
Bpu10I CCTNAGC 1 cut(s) 330
BpuEI CTTGAG 1 cut(s) 342
BpuMI CCSGG 1 cut(s) 272
Bsa29I ATCGAT 1 cut(s) 303
BsaHI GRCGYC 1 cut(s) 596
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 2 cut(s) 612, 613
BsaXI ACNNNNNCTCC 2 cut(s) 723, 753
Bsc4I CCNNNNNNNGG 1 cut(s) 432
Bse1I ACTGG 1 cut(s) 495
Bse3DI GCAATG 2 cut(s) 390, 781
BseBI CCWGG 2 cut(s) 614, 769
BseCI ATCGAT 1 cut(s) 303
BseDI CCNNGG 2 cut(s) 612, 613
BseGI GGATG 6 cut(s) 16, 151, 426, 441, 907, 982
BseLI CCNNNNNNNGG 1 cut(s) 432
BseMI GCAATG 2 cut(s) 390, 781
BseMII CTCAG 1 cut(s) 344
BseNI ACTGG 1 cut(s) 495
BseRI GAGGAG 7 cut(s) 86, 516, 519, 522, 525, 528, 878
BseXI GCAGC 2 cut(s) 338, 595
BshFI GGCC 6 cut(s) 185, 335, 355, 618, 678, 767
BshVI ATCGAT 1 cut(s) 303
BsiHKAI GWGCWC 1 cut(s) 401
BsiSI CCGG 1 cut(s) 272
BslFI GGGAC 3 cut(s) 583, 707, 833
BslI CCNNNNNNNGG 1 cut(s) 432
BsmAI GTCTC 1 cut(s) 209
BsmFI GGGAC 3 cut(s) 583, 707, 833
BsnI GGCC 6 cut(s) 185, 335, 355, 618, 678, 767
Bso31I GGTCTC 1 cut(s) 209
Bsp1286I GDGCHC 2 cut(s) 272, 401
Bsp1407I TGTACA 1 cut(s) 918
Bsp143I GATC 4 cut(s) 13, 150, 202, 438
BspACI CCGC 1 cut(s) 316
BspANI GGCC 6 cut(s) 185, 335, 355, 618, 678, 767
BspCNI CTCAG 1 cut(s) 343
BspDI ATCGAT 1 cut(s) 303
BspHI TCATGA 1 cut(s) 510
BspLI GGNNCC 3 cut(s) 269, 464, 723
BspPI GGATC 1 cut(s) 145
BspTI CTTAAG 1 cut(s) 761
BspTNI GGTCTC 1 cut(s) 209
BsrDI GCAATG 2 cut(s) 390, 781
BsrGI TGTACA 1 cut(s) 918
BsrI ACTGG 1 cut(s) 495
BssECI CCNNGG 2 cut(s) 612, 613
BssMI GATC 4 cut(s) 13, 150, 202, 438
BssNI GRCGYC 1 cut(s) 596
Bst2UI CCWGG 2 cut(s) 614, 769
Bst4CI ACNGT 1 cut(s) 591
Bst6I CTCTTC 1 cut(s) 207
BstACI GRCGYC 1 cut(s) 596
BstAFI CTTAAG 1 cut(s) 761
BstAUI TGTACA 1 cut(s) 918
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 2 cut(s) 330, 442
BstF5I GGATG 6 cut(s) 16, 151, 426, 441, 907, 982
BstKTI GATC 4 cut(s) 16, 153, 205, 441
BstMAI GTCTC 1 cut(s) 209
BstMBI GATC 4 cut(s) 13, 150, 202, 438
BstMWI GCNNNNNNNGC 4 cut(s) 191, 261, 332, 764
BstNI CCWGG 2 cut(s) 614, 769
BstNSI RCATGY 1 cut(s) 111
BstSCI CCNGG 3 cut(s) 270, 612, 767
BstV1I GCAGC 2 cut(s) 338, 595
BstX2I RGATCY 1 cut(s) 150
BstXI CCANNNNNNTGG 1 cut(s) 409
BstYI RGATCY 1 cut(s) 150
Bsu15I ATCGAT 1 cut(s) 303
BsuRI GGCC 6 cut(s) 185, 335, 355, 618, 678, 767
BsuTUI ATCGAT 1 cut(s) 303
BtrI CACGTC 1 cut(s) 960
BtsCI GGATG 6 cut(s) 16, 151, 426, 441, 907, 982
BtsI GCAGTG 1 cut(s) 798
BtsIMutI CAGTG 2 cut(s) 502, 798
Cac8I GCNNGC 1 cut(s) 313
CciI TCATGA 1 cut(s) 510
Cfr13I GGNCC 2 cut(s) 616, 721
ClaI ATCGAT 1 cut(s) 303
CseI GACGC 3 cut(s) 317, 332, 585
Csp6I GTAC 4 cut(s) 20, 517, 832, 919
CviAII CATG 8 cut(s) 108, 157, 187, 349, 394, 479, 511, 788
CviQI GTAC 4 cut(s) 20, 517, 832, 919
DdeI CTNAG 2 cut(s) 330, 442
DpnI GATC 4 cut(s) 15, 152, 204, 440
DpnII GATC 4 cut(s) 13, 150, 202, 438
DrdI GACNNNNNNGTC 1 cut(s) 479
DseDI GACNNNNNNGTC 1 cut(s) 479
EaeI YGGCCR 1 cut(s) 183
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Eco147I AGGCCT 3 cut(s) 335, 678, 767
Eco24I GRGCYC 1 cut(s) 272
Eco31I GGTCTC 1 cut(s) 209
Eco47I GGWCC 1 cut(s) 721
Eco57I CTGAAG 1 cut(s) 231
EcoRII CCWGG 2 cut(s) 612, 767
EcoT38I GRGCYC 1 cut(s) 272
FaeI CATG 8 cut(s) 111, 160, 190, 352, 397, 482, 514, 791
FaqI GGGAC 3 cut(s) 583, 707, 833
FatI CATG 8 cut(s) 107, 156, 186, 348, 393, 478, 510, 787
FbaI TGATCA 1 cut(s) 13
Fnu4HI GCNGC 3 cut(s) 316, 327, 609
FokI GGATG 6 cut(s) 23, 158, 413, 448, 894, 989
FriOI GRGCYC 1 cut(s) 272
Fsp4HI GCNGC 3 cut(s) 316, 327, 609
FspBI CTAG 3 cut(s) 50, 137, 680
GluI GCNGC 3 cut(s) 316, 327, 609
GsuI CTGGAG 2 cut(s) 790, 1017
HaeIII GGCC 6 cut(s) 185, 335, 355, 618, 678, 767
HapII CCGG 1 cut(s) 272
HgaI GACGC 3 cut(s) 317, 332, 585
Hin1I GRCGYC 1 cut(s) 596
Hin1II CATG 8 cut(s) 111, 160, 190, 352, 397, 482, 514, 791
HindIII AAGCTT 2 cut(s) 192, 292
HinfI GANTC 1 cut(s) 685
HpaII CCGG 1 cut(s) 272
HphI GGTGA 6 cut(s) 190, 331, 415, 579, 764, 883
Hpy188I TCNGA 3 cut(s) 43, 252, 690
Hpy188III TCNNGA 3 cut(s) 32, 511, 971
Hpy99I CGWCG 2 cut(s) 326, 595
HpyAV CCTTC 2 cut(s) 254, 346
HpyCH4III ACNGT 1 cut(s) 591
HpyCH4IV ACGT 1 cut(s) 959
HpyCH4V TGCA 6 cut(s) 371, 673, 758, 791, 799, 980
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 261, 332, 764
HpyF3I CTNAG 2 cut(s) 330, 442
HpySE526I ACGT 1 cut(s) 959
Hsp92I GRCGYC 1 cut(s) 596
Hsp92II CATG 8 cut(s) 111, 160, 190, 352, 397, 482, 514, 791
Ksp22I TGATCA 1 cut(s) 13
Kzo9I GATC 4 cut(s) 13, 150, 202, 438
LmnI GCTCC 7 cut(s) 66, 267, 404, 529, 731, 771, 904
Lsp1109I GCAGC 2 cut(s) 338, 595
LweI GCATC 6 cut(s) 309, 593, 745, 786, 916, 967
MaeI CTAG 3 cut(s) 50, 137, 680
MaeII ACGT 1 cut(s) 959
MaeIII GTNAC 2 cut(s) 89, 178
MalI GATC 4 cut(s) 15, 152, 204, 440
MboI GATC 4 cut(s) 13, 150, 202, 438
MboII GAAGA 2 cut(s) 182, 224
MflI RGATCY 1 cut(s) 150
MhlI GDGCHC 2 cut(s) 272, 401
MluCI AATT 3 cut(s) 27, 890, 986
MmeI TCCRAC 1 cut(s) 566
MseI TTAA 3 cut(s) 57, 564, 762
MspA1I CMGCKG 1 cut(s) 608
MspCI CTTAAG 1 cut(s) 761
MspI CCGG 1 cut(s) 272
MspR9I CCNGG 3 cut(s) 272, 614, 769
MvaI CCWGG 2 cut(s) 614, 769
MwoI GCNNNNNNNGC 4 cut(s) 191, 261, 332, 764
NciI CCSGG 1 cut(s) 272
NdeII GATC 4 cut(s) 13, 150, 202, 438
NlaIII CATG 8 cut(s) 111, 160, 190, 352, 397, 482, 514, 791
NlaIV GGNNCC 3 cut(s) 269, 464, 723
NmuCI GTSAC 1 cut(s) 178
NspI RCATGY 1 cut(s) 111
PagI TCATGA 1 cut(s) 510
PasI CCCWGGG 1 cut(s) 613
PceI AGGCCT 3 cut(s) 335, 678, 767
PfeI GAWTC 1 cut(s) 685
PflMI CCANNNNNTGG 1 cut(s) 432
PkrI GCNGC 3 cut(s) 317, 328, 610
PshBI ATTAAT 1 cut(s) 564
Psp6I CCWGG 2 cut(s) 612, 767
PspGI CCWGG 2 cut(s) 612, 767
PspN4I GGNNCC 3 cut(s) 269, 464, 723
PspPI GGNCC 2 cut(s) 616, 721
PsuI RGATCY 1 cut(s) 150
PvuII CAGCTG 1 cut(s) 608
RsaI GTAC 4 cut(s) 21, 518, 833, 920
RsaNI GTAC 4 cut(s) 20, 517, 832, 919
SaqAI TTAA 3 cut(s) 57, 564, 762
SatI GCNGC 3 cut(s) 316, 327, 609
Sau3AI GATC 4 cut(s) 13, 150, 202, 438
Sau96I GGNCC 2 cut(s) 616, 721
ScaI AGTACT 1 cut(s) 21
ScrFI CCNGG 3 cut(s) 272, 614, 769
SduI GDGCHC 2 cut(s) 272, 401
SfaNI GCATC 6 cut(s) 309, 593, 745, 786, 916, 967
SinI GGWCC 1 cut(s) 721
SmlI CTYRAG 2 cut(s) 357, 761
SmoI CTYRAG 2 cut(s) 357, 761
Sse9I AATT 3 cut(s) 27, 890, 986
SseBI AGGCCT 3 cut(s) 335, 678, 767
SsiI CCGC 1 cut(s) 316
SspI AATATT 1 cut(s) 562
SspMI CTAG 3 cut(s) 50, 137, 680
StuI AGGCCT 3 cut(s) 335, 678, 767
StyD4I CCNGG 3 cut(s) 270, 612, 767
TaaI ACNGT 1 cut(s) 591
TaiI ACGT 1 cut(s) 962
TaqI TCGA 3 cut(s) 303, 321, 984
TasI AATT 3 cut(s) 27, 890, 986
TatI WGTACW 3 cut(s) 19, 516, 918
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 1 cut(s) 685
Tru1I TTAA 3 cut(s) 57, 564, 762
Tru9I TTAA 3 cut(s) 57, 564, 762
TscAI CASTG 2 cut(s) 502, 798
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 2 cut(s) 326, 608
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 6 cut(s) 173, 203, 401, 499, 527, 776
TspRI CASTG 2 cut(s) 502, 798
Van91I CCANNNNNTGG 1 cut(s) 432
Vha464I CTTAAG 1 cut(s) 761
VpaK11BI GGWCC 1 cut(s) 721
VspI ATTAAT 1 cut(s) 564
XapI RAATTY 1 cut(s) 890
XceI RCATGY 1 cut(s) 111
XspI CTAG 3 cut(s) 50, 137, 680
ZrmI AGTACT 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.