pycom08g11710

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000834
Physical Location & Seq
Reverse (-)
9933268 .. 9935745
2478 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g11710.2

Sequence Viewer

Length: 936 bp
ATGGAGATGGATGATCAAGTACTCCCAATTCTTGATTTTTCAGATGGTTTAGTGGTAAAGCAAGGGAGTGAGGAATGGAAAGCTATGAGTAACAAAGTGAGAGAGGCATGTGAGGATTATGGTTGCTTTCTTTTGCTAGTGAAGGATGAGATCCCAATGAGTTTACGTGAAGAAATGGTGACGGCAATGAAAGCTTTGTTTGATCTGCCTGAAGAGACCAAGAAGAAGCACACAAGTCCCAAGCCTTACCAGAGCTACGAAGGCAAGAGCCCCGTCATTCCCTTGAATGAAAGCTTCGGCATCGATGACGCTTGCCGCCTCGACGCAGCTCAGACCTTCACCAACCTCATGTGGCCTCAAGAAAACCCTGCATTTTGCTTTGGTTTAGGAAACCACTATGACTCCCATGTCCAAAATACCACCAGTGTTTTTCGTATCATGAAGTACAAAGCTCCTCCTCCTCCTCCTCCTCCAACTCCAGGCAACATCAACATTAATGCTAACTCGTCATCAACATCACTGTCGGCGTCGCCATCAGCTGCCCTGGGCCTTGTACCTCACACTGACAAAAGCACCCTAACCATTCTGTGCCAAAACGGTGTGCAAGGCCTACAGATTCTGACCAAACAAGGCAAATGGGTTGAAGTGATGGTCCCGGAGGGAGCTGTTGTGGTCTTTGTTGGGGATGCAATTAAGGCTTGGAGCAATGGGAGGCTTCATGCAGTGACCCACAGGGTTATGATGAGTGGTGACAAAGAGAGGTACTCGTATGCCCTATTCTCCTTGCCAAAAGAGGAGGTGGTGATTGAAGTGCCTCAGGAATTTATAGACAAAGAGCATCCTTTGCTCTACAAGCCATTCAACTTTGGTGCATACTTTACATACTTCACGTCCAATATTCGTGAGGATGCACTCGAATTATATGCTAGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

312

Amino Acids

34.79

Weight (kDa)

5.25

Isoelectric Point (pI)

51.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 407
AciI CCGC 1 cut(s) 316
AclWI GGATC 1 cut(s) 145
AcsI RAATTY 1 cut(s) 821
AcuI CTGAAG 1 cut(s) 231
AcyI GRCGYC 1 cut(s) 527
AfaI GTAC 4 cut(s) 21, 446, 555, 764
AfiI CCNNNNNNNGG 1 cut(s) 479
AgsI TTSAA 4 cut(s) 286, 644, 809, 862
AjiI CACGTC 1 cut(s) 891
AjnI CCWGG 2 cut(s) 478, 543
AluBI AGCT 8 cut(s) 83, 194, 255, 294, 329, 452, 539, 665
AluI AGCT 8 cut(s) 83, 194, 255, 294, 329, 452, 539, 665
Alw26I GTCTC 1 cut(s) 209
AlwI GGATC 1 cut(s) 145
AlwNI CAGNNNCTG 1 cut(s) 619
AoxI GGCC 3 cut(s) 353, 547, 607
ApeKI GCWGC 2 cut(s) 326, 539
ApoI RAATTY 1 cut(s) 821
AseI ATTAAT 1 cut(s) 495
AspS9I GGNCC 2 cut(s) 547, 652
AsuC2I CCSGG 1 cut(s) 656
AsuHPI GGTGA 4 cut(s) 190, 331, 761, 814
AvaII GGWCC 1 cut(s) 652
AxyI CCTNAGG 1 cut(s) 816
BanII GRGCYC 1 cut(s) 272
BbvI GCAGC 2 cut(s) 338, 526
BccI CCATC 3 cut(s) 38, 541, 643
BceAI ACGGC 1 cut(s) 198
BcgI CGANNNNNNTGC 1 cut(s) 905
BciT130I CCWGG 2 cut(s) 480, 545
BclI TGATCA 1 cut(s) 13
BcnI CCSGG 1 cut(s) 656
BcoDI GTCTC 1 cut(s) 209
BfaI CTAG 2 cut(s) 137, 927
BfmI CTRYAG 1 cut(s) 611
BisI GCNGC 3 cut(s) 316, 327, 540
BlsI GCNGC 3 cut(s) 317, 328, 541
BmcAI AGTACT 1 cut(s) 21
Bme1390I CCNGG 3 cut(s) 480, 545, 656
Bme18I GGWCC 1 cut(s) 652
BmgBI CACGTC 1 cut(s) 891
BmgT120I GGNCC 2 cut(s) 547, 652
BmiI GGNNCC 1 cut(s) 654
BmrFI CCNGG 3 cut(s) 480, 545, 656
BmsI GCATC 4 cut(s) 309, 676, 847, 898
BplI GAGNNNNNCTC 2 cut(s) 749, 781
BpmI CTGGAG 1 cut(s) 462
BpuEI CTTGAG 1 cut(s) 342
BpuMI CCSGG 1 cut(s) 656
Bsa29I ATCGAT 1 cut(s) 303
BsaAI YACGTR 1 cut(s) 167
BsaHI GRCGYC 1 cut(s) 527
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 2 cut(s) 543, 544
BsaXI ACNNNNNCTCC 4 cut(s) 386, 416, 654, 684
Bsc4I CCNNNNNNNGG 1 cut(s) 479
Bse1I ACTGG 1 cut(s) 423
Bse21I CCTNAGG 1 cut(s) 816
Bse3DI GCAATG 2 cut(s) 192, 712
BseBI CCWGG 2 cut(s) 480, 545
BseCI ATCGAT 1 cut(s) 303
BseDI CCNNGG 2 cut(s) 543, 544
BseGI GGATG 5 cut(s) 16, 151, 691, 838, 913
BseLI CCNNNNNNNGG 1 cut(s) 479
BseMI GCAATG 2 cut(s) 192, 712
BseMII CTCAG 2 cut(s) 344, 830
BseNI ACTGG 1 cut(s) 423
BseRI GAGGAG 7 cut(s) 444, 447, 450, 453, 456, 459, 809
BseXI GCAGC 2 cut(s) 338, 526
BshFI GGCC 3 cut(s) 355, 549, 609
BshVI ATCGAT 1 cut(s) 303
BsiSI CCGG 1 cut(s) 656
BslFI GGGAC 2 cut(s) 222, 638
BslI CCNNNNNNNGG 1 cut(s) 479
BsmAI GTCTC 1 cut(s) 209
BsmFI GGGAC 2 cut(s) 222, 638
BsnI GGCC 3 cut(s) 355, 549, 609
Bso31I GGTCTC 1 cut(s) 209
Bsp1286I GDGCHC 1 cut(s) 272
Bsp143I GATC 3 cut(s) 13, 150, 202
BspACI CCGC 1 cut(s) 316
BspANI GGCC 3 cut(s) 355, 549, 609
BspCNI CTCAG 2 cut(s) 343, 829
BspDI ATCGAT 1 cut(s) 303
BspHI TCATGA 1 cut(s) 438
BspLI GGNNCC 1 cut(s) 654
BspPI GGATC 1 cut(s) 145
BspTNI GGTCTC 1 cut(s) 209
BsrDI GCAATG 2 cut(s) 192, 712
BsrI ACTGG 1 cut(s) 423
BssECI CCNNGG 2 cut(s) 543, 544
BssMI GATC 3 cut(s) 13, 150, 202
BssNI GRCGYC 1 cut(s) 527
Bst2UI CCWGG 2 cut(s) 480, 545
Bst4CI ACNGT 2 cut(s) 522, 599
Bst6I CTCTTC 1 cut(s) 207
BstACI GRCGYC 1 cut(s) 527
BstAPI GCANNNNNTGC 1 cut(s) 844
BstBAI YACGTR 1 cut(s) 167
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 2 cut(s) 330, 816
BstF5I GGATG 5 cut(s) 16, 151, 691, 838, 913
BstKTI GATC 3 cut(s) 16, 153, 205
BstMAI GTCTC 1 cut(s) 209
BstMBI GATC 3 cut(s) 13, 150, 202
BstMWI GCNNNNNNNGC 5 cut(s) 191, 261, 695, 844, 853
BstNI CCWGG 2 cut(s) 480, 545
BstNSI RCATGY 1 cut(s) 111
BstSCI CCNGG 3 cut(s) 478, 543, 654
BstSFI CTRYAG 1 cut(s) 611
BstV1I GCAGC 2 cut(s) 338, 526
BstX2I RGATCY 1 cut(s) 150
BstYI RGATCY 1 cut(s) 150
Bsu15I ATCGAT 1 cut(s) 303
Bsu36I CCTNAGG 1 cut(s) 816
BsuRI GGCC 3 cut(s) 355, 549, 609
BsuTUI ATCGAT 1 cut(s) 303
BtrI CACGTC 1 cut(s) 891
BtsCI GGATG 5 cut(s) 16, 151, 691, 838, 913
BtsI GCAGTG 1 cut(s) 729
BtsIMutI CAGTG 4 cut(s) 430, 518, 561, 729
Cac8I GCNNGC 1 cut(s) 313
CaiI CAGNNNCTG 1 cut(s) 619
CciI TCATGA 1 cut(s) 438
Cfr13I GGNCC 2 cut(s) 547, 652
ClaI ATCGAT 1 cut(s) 303
CseI GACGC 3 cut(s) 317, 332, 516
Csp6I GTAC 4 cut(s) 20, 445, 554, 763
CviAII CATG 5 cut(s) 108, 349, 407, 439, 719
CviQI GTAC 4 cut(s) 20, 445, 554, 763
DdeI CTNAG 2 cut(s) 330, 816
DpnI GATC 3 cut(s) 15, 152, 204
DpnII GATC 3 cut(s) 13, 150, 202
DrdI GACNNNNNNGTC 1 cut(s) 407
DseDI GACNNNNNNGTC 1 cut(s) 407
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Eco147I AGGCCT 1 cut(s) 609
Eco24I GRGCYC 1 cut(s) 272
Eco31I GGTCTC 1 cut(s) 209
Eco47I GGWCC 1 cut(s) 652
Eco57I CTGAAG 1 cut(s) 231
Eco81I CCTNAGG 1 cut(s) 816
EcoRII CCWGG 2 cut(s) 478, 543
EcoT38I GRGCYC 1 cut(s) 272
FaeI CATG 5 cut(s) 111, 352, 410, 442, 722
FaqI GGGAC 2 cut(s) 222, 638
FatI CATG 5 cut(s) 107, 348, 406, 438, 718
FbaI TGATCA 1 cut(s) 13
Fnu4HI GCNGC 3 cut(s) 316, 327, 540
FokI GGATG 5 cut(s) 23, 158, 698, 825, 920
FriOI GRGCYC 1 cut(s) 272
Fsp4HI GCNGC 3 cut(s) 316, 327, 540
FspBI CTAG 2 cut(s) 137, 927
GluI GCNGC 3 cut(s) 316, 327, 540
GsuI CTGGAG 1 cut(s) 462
HaeIII GGCC 3 cut(s) 355, 549, 609
HapII CCGG 1 cut(s) 656
HgaI GACGC 3 cut(s) 317, 332, 516
Hin1I GRCGYC 1 cut(s) 527
Hin1II CATG 5 cut(s) 111, 352, 410, 442, 722
HindIII AAGCTT 2 cut(s) 192, 292
HinfI GANTC 2 cut(s) 401, 616
HpaII CCGG 1 cut(s) 656
HphI GGTGA 4 cut(s) 190, 331, 761, 814
Hpy166II GTNNAC 1 cut(s) 164
Hpy188I TCNGA 3 cut(s) 43, 333, 621
Hpy188III TCNNGA 5 cut(s) 32, 359, 439, 818, 902
Hpy8I GTNNAC 1 cut(s) 164
Hpy99I CGWCG 2 cut(s) 326, 532
HpyAV CCTTC 3 cut(s) 136, 254, 346
HpyCH4III ACNGT 2 cut(s) 522, 599
HpyCH4IV ACGT 2 cut(s) 166, 890
HpyCH4V TGCA 6 cut(s) 371, 604, 689, 722, 872, 911
HpyF10VI GCNNNNNNNGC 5 cut(s) 191, 261, 695, 844, 853
HpyF3I CTNAG 2 cut(s) 330, 816
HpySE526I ACGT 2 cut(s) 166, 890
Hsp92I GRCGYC 1 cut(s) 527
Hsp92II CATG 5 cut(s) 111, 352, 410, 442, 722
Ksp22I TGATCA 1 cut(s) 13
Kzo9I GATC 3 cut(s) 13, 150, 202
LmnI GCTCC 3 cut(s) 457, 662, 702
Lsp1109I GCAGC 2 cut(s) 338, 526
LweI GCATC 4 cut(s) 309, 676, 847, 898
MaeI CTAG 2 cut(s) 137, 927
MaeII ACGT 2 cut(s) 166, 890
MaeIII GTNAC 4 cut(s) 89, 178, 724, 749
MalI GATC 3 cut(s) 15, 152, 204
MboI GATC 3 cut(s) 13, 150, 202
MboII GAAGA 3 cut(s) 182, 224, 235
MflI RGATCY 1 cut(s) 150
MhlI GDGCHC 1 cut(s) 272
MluCI AATT 4 cut(s) 27, 690, 821, 917
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 1 cut(s) 497
MseI TTAA 2 cut(s) 495, 693
MspA1I CMGCKG 1 cut(s) 539
MspI CCGG 1 cut(s) 656
MspR9I CCNGG 3 cut(s) 480, 545, 656
MvaI CCWGG 2 cut(s) 480, 545
MwoI GCNNNNNNNGC 5 cut(s) 191, 261, 695, 844, 853
NciI CCSGG 1 cut(s) 656
NdeII GATC 3 cut(s) 13, 150, 202
NlaIII CATG 5 cut(s) 111, 352, 410, 442, 722
NlaIV GGNNCC 1 cut(s) 654
NmuCI GTSAC 3 cut(s) 178, 724, 749
NspI RCATGY 1 cut(s) 111
PagI TCATGA 1 cut(s) 438
PasI CCCWGGG 1 cut(s) 544
PceI AGGCCT 1 cut(s) 609
PfeI GAWTC 1 cut(s) 616
PfoI TCCNGGA 1 cut(s) 654
PkrI GCNGC 3 cut(s) 317, 328, 541
PleI GAGTC 1 cut(s) 395
PpsI GAGTC 1 cut(s) 395
Ppu21I YACGTR 1 cut(s) 167
PshBI ATTAAT 1 cut(s) 495
Psp6I CCWGG 2 cut(s) 478, 543
PspGI CCWGG 2 cut(s) 478, 543
PspN4I GGNNCC 1 cut(s) 654
PspPI GGNCC 2 cut(s) 547, 652
PstNI CAGNNNCTG 1 cut(s) 619
PsuI RGATCY 1 cut(s) 150
PvuII CAGCTG 1 cut(s) 539
RsaI GTAC 4 cut(s) 21, 446, 555, 764
RsaNI GTAC 4 cut(s) 20, 445, 554, 763
SaqAI TTAA 2 cut(s) 495, 693
SatI GCNGC 3 cut(s) 316, 327, 540
Sau3AI GATC 3 cut(s) 13, 150, 202
Sau96I GGNCC 2 cut(s) 547, 652
ScaI AGTACT 1 cut(s) 21
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 3 cut(s) 480, 545, 656
SduI GDGCHC 1 cut(s) 272
SfaNI GCATC 4 cut(s) 309, 676, 847, 898
SfcI CTRYAG 1 cut(s) 611
SinI GGWCC 1 cut(s) 652
SmlI CTYRAG 1 cut(s) 357
SmoI CTYRAG 1 cut(s) 357
Sse9I AATT 4 cut(s) 27, 690, 821, 917
SseBI AGGCCT 1 cut(s) 609
SsiI CCGC 1 cut(s) 316
SspI AATATT 1 cut(s) 898
SspMI CTAG 2 cut(s) 137, 927
StuI AGGCCT 1 cut(s) 609
StyD4I CCNGG 3 cut(s) 478, 543, 654
TaaI ACNGT 2 cut(s) 522, 599
TaiI ACGT 2 cut(s) 169, 893
TaqI TCGA 3 cut(s) 303, 321, 915
TasI AATT 4 cut(s) 27, 690, 821, 917
TatI WGTACW 2 cut(s) 19, 444
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 1 cut(s) 616
Tru1I TTAA 2 cut(s) 495, 693
Tru9I TTAA 2 cut(s) 495, 693
TscAI CASTG 4 cut(s) 430, 525, 568, 729
TseFI GTSAC 3 cut(s) 178, 724, 749
TseI GCWGC 2 cut(s) 326, 539
Tsp45I GTSAC 3 cut(s) 178, 724, 749
TspDTI ATGAA 4 cut(s) 203, 303, 455, 707
TspRI CASTG 4 cut(s) 430, 525, 568, 729
VpaK11BI GGWCC 1 cut(s) 652
VspI ATTAAT 1 cut(s) 495
XapI RAATTY 1 cut(s) 821
XceI RCATGY 1 cut(s) 111
XspI CTAG 2 cut(s) 137, 927
ZrmI AGTACT 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.