RchiOBHm_Chr5g0019681

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000834
Physical Location & Seq
Reverse (-)
14106014 .. 14110484
4471 bp
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UTR
Exon/CDS
Intron
PRQ29981

Sequence Viewer

Length: 444 bp
ATGATGAAGCTTCCTCTTGAATTTTCTAGTGAGTTTTTAGTCTCAAAGCAAGGGAGTGAGGAATGGAAAGCTATGAGGAACAAAGTGAGAGAGGCATGTCAGAGTTATGGTTGCTTTCTGCTGTTGGTGCGGGAAGAGACAATCCCCATCAACTTACGTGAAGAAATGGTGATGACCATGAAGGGTTTGTTCGATCTCCTTGAACAGACCAAGCAGAAGCACAAAAGTACGAACTCTTTCCGTGCCTACCAAGGCAAATCCCCCAACTTTCCATTGAGCGAAAGCTTTGGAATTGATAGTTCAGACCAGATTGATGCAGCTCAAGCCTTCACCAACCTCATTGAGATAATGAAATTAATGAGCTCAAAGCTGATGGATTTGAACTACTTCACCATTGTCAAAATGATTTTTGAGAGCTTTGGCATTGAGAAGCACTACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

147

Amino Acids

17.09

Weight (kDa)

6.74

Isoelectric Point (pI)

54.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 18 - 96 5.9e-10 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 130
AcsI RAATTY 1 cut(s) 20
AfaI GTAC 1 cut(s) 229
AgsI TTSAA 3 cut(s) 20, 203, 382
AluBI AGCT 7 cut(s) 10, 71, 285, 320, 363, 370, 417
AluI AGCT 7 cut(s) 10, 71, 285, 320, 363, 370, 417
Alw21I GWGCWC 1 cut(s) 365
Alw26I GTCTC 2 cut(s) 46, 131
ApeKI GCWGC 1 cut(s) 317
ApoI RAATTY 1 cut(s) 20
AseI ATTAAT 1 cut(s) 356
Asp700I GAANNNNTTC 2 cut(s) 236, 386
AsuHPI GGTGA 3 cut(s) 181, 322, 382
BanII GRGCYC 1 cut(s) 365
Bbv12I GWGCWC 1 cut(s) 365
BbvI GCAGC 1 cut(s) 329
BccI CCATC 2 cut(s) 155, 367
BcoDI GTCTC 2 cut(s) 46, 131
BfaI CTAG 1 cut(s) 27
BisI GCNGC 1 cut(s) 318
BlsI GCNGC 1 cut(s) 319
BmsI GCATC 1 cut(s) 304
BpuEI CTTGAG 1 cut(s) 306
BsaAI YACGTR 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 250
BseDI CCNNGG 1 cut(s) 250
BseXI GCAGC 1 cut(s) 329
BsiHKAI GWGCWC 1 cut(s) 365
BsmAI GTCTC 2 cut(s) 46, 131
Bsp1286I GDGCHC 1 cut(s) 365
Bsp143I GATC 1 cut(s) 193
BspACI CCGC 1 cut(s) 130
BssECI CCNNGG 1 cut(s) 250
BssMI GATC 1 cut(s) 193
BssT1I CCWWGG 1 cut(s) 250
Bst6I CTCTTC 1 cut(s) 129
BstBAI YACGTR 1 cut(s) 158
BstKTI GATC 1 cut(s) 196
BstMAI GTCTC 2 cut(s) 46, 131
BstMBI GATC 1 cut(s) 193
BstMWI GCNNNNNNNGC 2 cut(s) 127, 323
BstNSI RCATGY 1 cut(s) 99
BstV1I GCAGC 1 cut(s) 329
Csp6I GTAC 1 cut(s) 228
CviAII CATG 2 cut(s) 96, 178
CviJI RGCY 8 cut(s) 10, 71, 285, 320, 326, 363, 370, 417
CviKI_1 RGCY 8 cut(s) 10, 71, 285, 320, 326, 363, 370, 417
CviQI GTAC 1 cut(s) 228
DpnI GATC 1 cut(s) 195
DpnII GATC 1 cut(s) 193
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
Ecl136II GAGCTC 1 cut(s) 363
Eco130I CCWWGG 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 365
Eco53kI GAGCTC 1 cut(s) 363
EcoICRI GAGCTC 1 cut(s) 363
EcoT14I CCWWGG 1 cut(s) 250
EcoT38I GRGCYC 1 cut(s) 365
ErhI CCWWGG 1 cut(s) 250
FaeI CATG 2 cut(s) 99, 181
FaiI YATR 4 cut(s) 74, 97, 108, 179
FatI CATG 2 cut(s) 95, 177
FauI CCCGC 1 cut(s) 123
Fnu4HI GCNGC 1 cut(s) 318
FriOI GRGCYC 1 cut(s) 365
Fsp4HI GCNGC 1 cut(s) 318
FspBI CTAG 1 cut(s) 27
GluI GCNGC 1 cut(s) 318
Hin1II CATG 2 cut(s) 99, 181
HindIII AAGCTT 2 cut(s) 8, 283
HphI GGTGA 3 cut(s) 181, 322, 382
Hpy188I TCNGA 2 cut(s) 102, 304
Hpy188III TCNNGA 1 cut(s) 17
HpyAV CCTTC 2 cut(s) 175, 337
HpyCH4IV ACGT 1 cut(s) 157
HpyCH4V TGCA 1 cut(s) 317
HpyF10VI GCNNNNNNNGC 2 cut(s) 127, 323
HpySE526I ACGT 1 cut(s) 157
Hsp92II CATG 2 cut(s) 99, 181
Kzo9I GATC 1 cut(s) 193
LpnPI CCDG 1 cut(s) 320
Lsp1109I GCAGC 1 cut(s) 329
LweI GCATC 1 cut(s) 304
MaeI CTAG 1 cut(s) 27
MaeII ACGT 1 cut(s) 157
MalI GATC 1 cut(s) 195
MboI GATC 1 cut(s) 193
MboII GAAGA 2 cut(s) 146, 173
MhlI GDGCHC 1 cut(s) 365
MluCI AATT 3 cut(s) 20, 291, 353
MnlI CCTC 5 cut(s) 24, 52, 69, 85, 347
MroXI GAANNNNTTC 2 cut(s) 236, 386
MseI TTAA 1 cut(s) 356
MwoI GCNNNNNNNGC 2 cut(s) 127, 323
NdeII GATC 1 cut(s) 193
NlaIII CATG 2 cut(s) 99, 181
NspI RCATGY 1 cut(s) 99
PdmI GAANNNNTTC 2 cut(s) 236, 386
PkrI GCNGC 1 cut(s) 319
Ppu21I YACGTR 1 cut(s) 158
PshBI ATTAAT 1 cut(s) 356
Psp124BI GAGCTC 1 cut(s) 365
RsaI GTAC 1 cut(s) 229
RsaNI GTAC 1 cut(s) 228
SacI GAGCTC 1 cut(s) 365
SaqAI TTAA 1 cut(s) 356
SatI GCNGC 1 cut(s) 318
Sau3AI GATC 1 cut(s) 193
SduI GDGCHC 1 cut(s) 365
SetI ASST 9 cut(s) 12, 73, 160, 287, 322, 339, 365, 372, 419
SfaNI GCATC 1 cut(s) 304
SmlI CTYRAG 1 cut(s) 321
SmoI CTYRAG 1 cut(s) 321
Sse9I AATT 3 cut(s) 20, 291, 353
SsiI CCGC 1 cut(s) 130
SspMI CTAG 1 cut(s) 27
SstI GAGCTC 1 cut(s) 365
StyI CCWWGG 1 cut(s) 250
TaiI ACGT 1 cut(s) 160
TaqI TCGA 1 cut(s) 192
TasI AATT 3 cut(s) 20, 291, 353
Tru1I TTAA 1 cut(s) 356
Tru9I TTAA 1 cut(s) 356
TseI GCWGC 1 cut(s) 317
TspDTI ATGAA 3 cut(s) 20, 194, 365
TspGWI ACGGA 1 cut(s) 230
VspI ATTAAT 1 cut(s) 356
XapI RAATTY 1 cut(s) 20
XceI RCATGY 1 cut(s) 99
XmnI GAANNNNTTC 2 cut(s) 236, 386
XspI CTAG 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.