MD10G1236100.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003818
Physical Location & Seq
Forward (+)
33262623 .. 33263153
531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1236100.v1.1.491

Sequence Viewer

Length: 531 bp
ATGAATTTAAGACTTTGTTGGTTTTTTATTGTATCATTGTGTAACTATTTGAATAAGAAATCAGTACGTCCTGTTCCATTACAAATTGTTGAATATTTACTGAGCTTTTCGTGTCCCTCATTTTTCAGGTGTGGGCATGAGTTCTGTTATTCTTGTGGTGCTGAATATCGGGATAGCCAACAGACTTGTCAATGTGCCTTTTGGGACGAAGACAACACCACCATAGAAGACTTGGTCACTCAGTCTATGCAAGAATCCGAGCAATGGGCGTGGGAAACATTCAATTCACTCCCCATGATAATGGATGCATACTCGGACCAAGAGAGATCGCAGCTGGCGCTGATCCAGAGATTCCTAGATTGGGGGGGGGGTTTCAGTTTAAACCATCCCCCTTATCAATCCCCACCTCGGTGTACAGATTCTTATGTAGACGCCATGAAAGATCTCCATCCGCTTCCTTGGCTCGAGAGGTTTGTTTTCGTTATAAGTGATAACTACTACAAAATTATATTTGCTATGTGGAATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR RVT_3 zf-C3HC4

Protein Analysis

177

Amino Acids

20.85

Weight (kDa)

4.65

Isoelectric Point (pI)

51.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 485
AccI GTMKAC 1 cut(s) 429
AciI CCGC 1 cut(s) 452
AclWI GGATC 1 cut(s) 337
AcsI RAATTY 2 cut(s) 4, 523
AcyI GRCGYC 1 cut(s) 432
AfaI GTAC 2 cut(s) 66, 415
AfiI CCNNNNNNNGG 3 cut(s) 264, 361, 408
AgsI TTSAA 3 cut(s) 52, 92, 283
AleI CACNNNNGTG 1 cut(s) 409
AluBI AGCT 2 cut(s) 105, 334
AluI AGCT 2 cut(s) 105, 334
AlwI GGATC 1 cut(s) 337
Ama87I CYCGRG 1 cut(s) 464
ApeKI GCWGC 1 cut(s) 331
ApoI RAATTY 2 cut(s) 4, 523
AspLEI GCGC 1 cut(s) 340
AspS9I GGNCC 1 cut(s) 316
AvaI CYCGRG 1 cut(s) 464
AvaII GGWCC 1 cut(s) 316
BbsI GAAGAC 2 cut(s) 216, 234
BbvI GCAGC 1 cut(s) 343
BccI CCATC 2 cut(s) 393, 456
BfaI CTAG 1 cut(s) 356
BfoI RGCGCY 1 cut(s) 341
BglII AGATCT 1 cut(s) 442
BisI GCNGC 1 cut(s) 332
BlsI GCNGC 1 cut(s) 333
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 1 cut(s) 464
BmgT120I GGNCC 1 cut(s) 316
BmsI GCATC 1 cut(s) 295
BpiI GAAGAC 2 cut(s) 216, 234
BsaBI GATNNNNATC 1 cut(s) 447
BsaHI GRCGYC 1 cut(s) 432
BsaJI CCNNGG 2 cut(s) 407, 458
Bsc4I CCNNNNNNNGG 3 cut(s) 264, 361, 408
Bse3DI GCAATG 1 cut(s) 269
Bse8I GATNNNNATC 1 cut(s) 447
BseDI CCNNGG 2 cut(s) 407, 458
BseGI GGATG 3 cut(s) 310, 385, 448
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 3 cut(s) 264, 361, 408
BseMI GCAATG 1 cut(s) 269
BseMII CTCAG 2 cut(s) 92, 254
BseXI GCAGC 1 cut(s) 343
BsiHKCI CYCGRG 1 cut(s) 464
BslFI GGGAC 2 cut(s) 99, 218
BslI CCNNNNNNNGG 3 cut(s) 264, 361, 408
BsmFI GGGAC 2 cut(s) 99, 218
BsoBI CYCGRG 1 cut(s) 464
Bsp1407I TGTACA 1 cut(s) 413
Bsp143I GATC 3 cut(s) 326, 342, 442
BspACI CCGC 1 cut(s) 452
BspCNI CTCAG 2 cut(s) 93, 253
BspPI GGATC 1 cut(s) 337
BsrDI GCAATG 1 cut(s) 269
BsrGI TGTACA 1 cut(s) 413
BssECI CCNNGG 2 cut(s) 407, 458
BssMI GATC 3 cut(s) 326, 342, 442
BssNI GRCGYC 1 cut(s) 432
BssT1I CCWWGG 1 cut(s) 458
BstACI GRCGYC 1 cut(s) 432
BstAUI TGTACA 1 cut(s) 413
BstC8I GCNNGC 1 cut(s) 336
BstDEI CTNAG 2 cut(s) 101, 240
BstF5I GGATG 3 cut(s) 310, 385, 448
BstH2I RGCGCY 1 cut(s) 341
BstHHI GCGC 1 cut(s) 340
BstKTI GATC 3 cut(s) 329, 345, 445
BstMBI GATC 3 cut(s) 326, 342, 442
BstMWI GCNNNNNNNGC 2 cut(s) 337, 460
BstV1I GCAGC 1 cut(s) 343
BstV2I GAAGAC 2 cut(s) 216, 234
BstX2I RGATCY 1 cut(s) 442
BstXI CCANNNNNNTGG 1 cut(s) 301
BstYI RGATCY 1 cut(s) 442
BtsCI GGATG 3 cut(s) 310, 385, 448
Cac8I GCNNGC 1 cut(s) 336
CfoI GCGC 1 cut(s) 340
Cfr13I GGNCC 1 cut(s) 316
CseI GACGC 1 cut(s) 440
Csp6I GTAC 2 cut(s) 65, 414
CspCI CAANNNNNGTGG 2 cut(s) 251, 286
CviAII CATG 3 cut(s) 137, 295, 436
CviJI RGCY 4 cut(s) 105, 177, 334, 463
CviKI_1 RGCY 4 cut(s) 105, 177, 334, 463
CviQI GTAC 2 cut(s) 65, 414
DdeI CTNAG 2 cut(s) 101, 240
DpnI GATC 3 cut(s) 328, 344, 444
DpnII GATC 3 cut(s) 326, 342, 442
DraI TTTAAA 1 cut(s) 381
Eco130I CCWWGG 1 cut(s) 458
Eco47I GGWCC 1 cut(s) 316
Eco88I CYCGRG 1 cut(s) 464
EcoT14I CCWWGG 1 cut(s) 458
EcoT22I ATGCAT 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 458
FaeI CATG 3 cut(s) 140, 298, 439
FaqI GGGAC 2 cut(s) 99, 218
FatI CATG 3 cut(s) 136, 294, 435
FblI GTMKAC 1 cut(s) 429
Fnu4HI GCNGC 1 cut(s) 332
FokI GGATG 3 cut(s) 317, 372, 435
Fsp4HI GCNGC 1 cut(s) 332
FspBI CTAG 1 cut(s) 356
GlaI GCGC 1 cut(s) 339
GluI GCNGC 1 cut(s) 332
HaeII RGCGCY 1 cut(s) 341
HgaI GACGC 1 cut(s) 440
HhaI GCGC 1 cut(s) 340
Hin1I GRCGYC 1 cut(s) 432
Hin1II CATG 3 cut(s) 140, 298, 439
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HinfI GANTC 3 cut(s) 254, 351, 419
Hpy166II GTNNAC 2 cut(s) 414, 430
Hpy188I TCNGA 2 cut(s) 259, 316
Hpy188III TCNNGA 3 cut(s) 170, 346, 466
Hpy8I GTNNAC 2 cut(s) 414, 430
HpyCH4IV ACGT 1 cut(s) 67
HpyCH4V TGCA 2 cut(s) 250, 308
HpyF10VI GCNNNNNNNGC 2 cut(s) 337, 460
HpyF3I CTNAG 2 cut(s) 101, 240
HpySE526I ACGT 1 cut(s) 67
Hsp92I GRCGYC 1 cut(s) 432
Hsp92II CATG 3 cut(s) 140, 298, 439
HspAI GCGC 1 cut(s) 338
Kzo9I GATC 3 cut(s) 326, 342, 442
LpnPI CCDG 4 cut(s) 84, 112, 320, 359
Lsp1109I GCAGC 1 cut(s) 343
LweI GCATC 1 cut(s) 295
MaeI CTAG 1 cut(s) 356
MaeII ACGT 1 cut(s) 67
MaeIII GTNAC 2 cut(s) 41, 235
MalI GATC 3 cut(s) 328, 344, 444
MboI GATC 3 cut(s) 326, 342, 442
MboII GAAGA 2 cut(s) 221, 239
MflI RGATCY 1 cut(s) 442
MluCI AATT 5 cut(s) 4, 84, 283, 504, 523
MnlI CCTC 3 cut(s) 127, 417, 462
Mph1103I ATGCAT 1 cut(s) 310
MseI TTAA 2 cut(s) 8, 380
MslI CAYNNNNRTG 2 cut(s) 299, 409
MspA1I CMGCKG 1 cut(s) 334
MssI GTTTAAAC 1 cut(s) 381
MwoI GCNNNNNNNGC 2 cut(s) 337, 460
NdeII GATC 3 cut(s) 326, 342, 442
NlaIII CATG 3 cut(s) 140, 298, 439
NmuCI GTSAC 1 cut(s) 235
NsiI ATGCAT 1 cut(s) 310
OliI CACNNNNGTG 1 cut(s) 409
PaeR7I CTCGAG 1 cut(s) 464
PfeI GAWTC 3 cut(s) 254, 351, 419
PflFI GACNNNGTC 1 cut(s) 233
PkrI GCNGC 1 cut(s) 333
PmeI GTTTAAAC 1 cut(s) 381
PsiI TTATAA 1 cut(s) 485
PspPI GGNCC 1 cut(s) 316
PsrI GAACNNNNNNTAC 2 cut(s) 57, 89
PsuI RGATCY 1 cut(s) 442
PsyI GACNNNGTC 1 cut(s) 233
PvuII CAGCTG 1 cut(s) 334
RsaI GTAC 2 cut(s) 66, 415
RsaNI GTAC 2 cut(s) 65, 414
RseI CAYNNNNRTG 2 cut(s) 299, 409
SaqAI TTAA 2 cut(s) 8, 380
SatI GCNGC 1 cut(s) 332
Sau3AI GATC 3 cut(s) 326, 342, 442
Sau96I GGNCC 1 cut(s) 316
SetI ASST 6 cut(s) 70, 107, 131, 336, 409, 473
SfaNI GCATC 1 cut(s) 295
Sfr274I CTCGAG 1 cut(s) 464
SinI GGWCC 1 cut(s) 316
SlaI CTCGAG 1 cut(s) 464
SmiMI CAYNNNNRTG 2 cut(s) 299, 409
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
Sse9I AATT 5 cut(s) 4, 84, 283, 504, 523
SsiI CCGC 1 cut(s) 452
SspI AATATT 1 cut(s) 95
SspMI CTAG 1 cut(s) 356
StyI CCWWGG 1 cut(s) 458
TaiI ACGT 1 cut(s) 70
TaqI TCGA 1 cut(s) 465
TasI AATT 5 cut(s) 4, 84, 283, 504, 523
TatI WGTACW 1 cut(s) 413
TfiI GAWTC 3 cut(s) 254, 351, 419
Tru1I TTAA 2 cut(s) 8, 380
Tru9I TTAA 2 cut(s) 8, 380
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 1 cut(s) 331
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 2 cut(s) 17, 452
Tth111I GACNNNGTC 1 cut(s) 233
VpaK11BI GGWCC 1 cut(s) 316
XapI RAATTY 2 cut(s) 4, 523
XcmI CCANNNNNNNNNTGG 1 cut(s) 229
XhoI CTCGAG 1 cut(s) 464
XmiI GTMKAC 1 cut(s) 429
XspI CTAG 1 cut(s) 356
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.