MD10G1281400.v1.1

Nucleoporin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011206
Physical Location & Seq
Reverse (-)
37201121 .. 37203464
2344 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1281400.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGCCGATCTCAGAGCCTCCGCAGATCCACCGACTCCCACAATCCAAGTACATAGACTCAGTCCGGTGGCTCCCGCCTCTCTCCGCCTTGGACCGCCTCGCCGTTATTGCAGTTTTCGACTCCGATTCCGACTCTTCCTCCTTAGAGACCCACTCTTTCACTCCGAACCCAGCAACCCTAACCCCGCAATCTAAATGGGTCTCGCCCTCCCGAATTTCCTCCCTCAAAACCTCCCAATCCCACCACAAACCCCTCGTCGCCGCCGCGACATTTTCCGGCTCCCTCCACATCTTGTACGGCGATTCAATGGACCCTGCGTCTCTCCAATCGGAGCTTTCGGTGCCCGAAAAGGAGTTACACGAGGGACCCATTTCTTGTGTGGATGTTATGGATGGTGGGTCGGAGTGTGTGAGTGTTGGGGAAGATGGGAGGGTCAATTTGGTAAGTTTAGGGGACTCTGAGTTGAGCTACCGGCGAGTTTTCGACAGCAGCGGCTTGGTTTCGTTTACCGCGGCGAAATGGGCATCTCCGAGTGAGTTTGCTACTGGTGGATATGGGTTTAGCCTCCAGTGGTGGGATCAGAGGAAGCGCGGCGGAGCGGTTTCTCAATTTAAGGGTGGCTGGTCTCAACCGTCAACTCCAGGGATTGTACATTCAATTGATATCCATCCTTCAAGAAAGCACACTTGTTTGGCTGGAGGTTCTTTAGGGACTGTATTTGCATGGGATCTTAGGTGGCCACAGCAGCCAATCCTTCTTTCTGGGGTTGGGGCAGGTGAAGCAACCCATTCACCATGCGAAAGTGAGGTTTGGGAGGTTCAGTACGACCACTATGCAAAGTCTTCGAATGTGGGAAATGTTTCTTCACGTATCTTACCTACTATGATATGTTCAGAAGACGGTATCCTTGCTGTTGTTGAACAAGGTGAGGAACCCATCGAGCTCCTGGCAGAACCTTGTGCAATCAACAGCTTTGACATAGACCGGCAATATCCTTCTGATGTTATATGTAGCTTGGAGTGGGAATCTATAGTCATCTTGACAAGGCCATGA

Protein Analysis

351

Amino Acids

37.99

Weight (kDa)

4.93

Isoelectric Point (pI)

66.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011206)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30840
fragaria_vesca FvH4_4g09770 FvH4_4g09770 FvH4_4g09770
malus_domestica MD10G1281400.v1.1
prunus_persica Prupe.1G102000_v2.0.a1 Prupe.1G102000_v2.0.a1 Prupe.1G102000_v2.0.a1
pyrus_communis pycom10g23530
rosa_chinensis RchiOBHm_Chr4g0405491
rosa_laevigata RLG00000008840
rosa_multiflora Rmu_co8147422.1_g000001
rosa_roxburghii Rroxscaffold_5G00349930
rosa_rugosa Rorug04G0060500 Rorug04G0060600
rosa_samantha Rh4AG132200 Rh4BG127000 Rh4CG139700 Rh4DG126300
rosa_wichuraiana Rw4G010700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 764
Acc36I ACCTGC 1 cut(s) 764
AccB1I GGYRCC 1 cut(s) 340
AccBSI CCGCTC 1 cut(s) 599
AccII CGCG 3 cut(s) 266, 512, 591
AclWI GGATC 3 cut(s) 19, 585, 735
AcoI YGGCCR 1 cut(s) 737
AcsI RAATTY 1 cut(s) 213
AfaI GTAC 4 cut(s) 50, 296, 651, 824
AfiI CCNNNNNNNGG 1 cut(s) 574
AgsI TTSAA 4 cut(s) 306, 657, 675, 920
AhdI GACNNNNNGTC 1 cut(s) 316
AjnI CCWGG 2 cut(s) 640, 945
AjuI GAANNNNNNNTTGG 2 cut(s) 742, 774
AluBI AGCT 5 cut(s) 334, 468, 943, 972, 1014
AluI AGCT 5 cut(s) 334, 468, 943, 972, 1014
Alw21I GWGCWC 1 cut(s) 945
Alw26I GTCTC 4 cut(s) 140, 205, 324, 630
AlwI GGATC 3 cut(s) 19, 585, 735
AoxI GGCC 2 cut(s) 737, 1046
ApeKI GCWGC 2 cut(s) 489, 745
ApoI RAATTY 1 cut(s) 213
ArsI GACNNNNNNTTYG 2 cut(s) 240, 272
Asp700I GAANNNNTTC 1 cut(s) 859
AspLEI GCGC 1 cut(s) 591
AspS9I GGNCC 3 cut(s) 91, 310, 365
AsuHPI GGTGA 3 cut(s) 783, 788, 938
AsuII TTCGAA 1 cut(s) 845
AvaII GGWCC 3 cut(s) 91, 310, 365
BaeGI GKGCMC 1 cut(s) 345
BalI TGGCCA 1 cut(s) 739
BanI GGYRCC 1 cut(s) 340
BanII GRGCYC 1 cut(s) 945
BauI CACGAG 1 cut(s) 359
BbsI GAAGAC 2 cut(s) 834, 903
Bbv12I GWGCWC 1 cut(s) 945
BbvI GCAGC 2 cut(s) 501, 757
BccI CCATC 4 cut(s) 386, 419, 675, 944
BceAI ACGGC 2 cut(s) 86, 313
BcgI CGANNNNNNTGC 4 cut(s) 815, 825, 849, 859
BciT130I CCWGG 2 cut(s) 642, 947
BciVI GTATCC 1 cut(s) 914
BcoDI GTCTC 4 cut(s) 140, 205, 324, 630
BfmI CTRYAG 1 cut(s) 1029
BfuAI ACCTGC 1 cut(s) 764
BfuI GTATCC 1 cut(s) 914
BisI GCNGC 7 cut(s) 261, 264, 490, 493, 513, 592, 746
BlsI GCNGC 7 cut(s) 262, 265, 491, 494, 514, 593, 747
Bme1390I CCNGG 2 cut(s) 642, 947
Bme18I GGWCC 3 cut(s) 91, 310, 365
BmeRI GACNNNNNGTC 1 cut(s) 316
BmgT120I GGNCC 3 cut(s) 91, 310, 365
BmiI GGNNCC 7 cut(s) 71, 280, 312, 342, 366, 367, 933
BmrFI CCNGG 2 cut(s) 642, 947
BmsI GCATC 1 cut(s) 533
BpiI GAAGAC 2 cut(s) 834, 903
BplI GAGNNNNNCTC 2 cut(s) 137, 169
BpmI CTGGAG 3 cut(s) 551, 624, 717
Bpu14I TTCGAA 1 cut(s) 845
BsaAI YACGTR 1 cut(s) 869
BsaBI GATNNNNATC 1 cut(s) 666
BsaI GGTCTC 3 cut(s) 140, 205, 630
BsaJI CCNNGG 3 cut(s) 87, 510, 641
BsaWI WCCGGW 1 cut(s) 63
BsaXI ACNNNNNCTCC 4 cut(s) 122, 152, 806, 836
Bsc4I CCNNNNNNNGG 1 cut(s) 574
Bse118I RCCGGY 2 cut(s) 471, 984
Bse1I ACTGG 2 cut(s) 550, 568
Bse8I GATNNNNATC 1 cut(s) 666
BseBI CCWGG 2 cut(s) 642, 947
BseDI CCNNGG 3 cut(s) 87, 510, 641
BseGI GGATG 3 cut(s) 388, 397, 667
BseJI GATNNNNATC 1 cut(s) 666
BseLI CCNNNNNNNGG 1 cut(s) 574
BseMII CTCAG 3 cut(s) 24, 72, 450
BseNI ACTGG 2 cut(s) 550, 568
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 2 cut(s) 501, 757
BseYI CCCAGC 1 cut(s) 169
Bsh1236I CGCG 3 cut(s) 266, 512, 591
BshFI GGCC 2 cut(s) 739, 1048
BshNI GGYRCC 1 cut(s) 340
BsiHKAI GWGCWC 1 cut(s) 945
BsiSI CCGG 4 cut(s) 64, 276, 472, 985
BslFI GGGAC 3 cut(s) 378, 467, 724
BslI CCNNNNNNNGG 1 cut(s) 574
BsmAI GTCTC 4 cut(s) 140, 205, 324, 630
BsmBI CGTCTC 1 cut(s) 324
BsmFI GGGAC 3 cut(s) 378, 467, 724
BsnI GGCC 2 cut(s) 739, 1048
Bso31I GGTCTC 3 cut(s) 140, 205, 630
Bsp119I TTCGAA 1 cut(s) 845
Bsp1286I GDGCHC 2 cut(s) 345, 945
Bsp1407I TGTACA 1 cut(s) 649
Bsp143I GATC 4 cut(s) 6, 24, 577, 727
BspANI GGCC 2 cut(s) 739, 1048
BspCNI CTCAG 3 cut(s) 23, 71, 451
BspFNI CGCG 3 cut(s) 266, 512, 591
BspLI GGNNCC 7 cut(s) 71, 280, 312, 342, 366, 367, 933
BspMI ACCTGC 1 cut(s) 764
BspPI GGATC 3 cut(s) 19, 585, 735
BspT104I TTCGAA 1 cut(s) 845
BspT107I GGYRCC 1 cut(s) 340
BspTNI GGTCTC 3 cut(s) 140, 205, 630
BsrBI CCGCTC 1 cut(s) 599
BsrFI RCCGGY 2 cut(s) 471, 984
BsrGI TGTACA 1 cut(s) 649
BsrI ACTGG 2 cut(s) 550, 568
BssAI RCCGGY 2 cut(s) 471, 984
BssECI CCNNGG 3 cut(s) 87, 510, 641
BssMI GATC 4 cut(s) 6, 24, 577, 727
BssSI CACGAG 1 cut(s) 359
BssT1I CCWWGG 1 cut(s) 87
Bst2BI CACGAG 1 cut(s) 359
Bst2UI CCWGG 2 cut(s) 642, 947
Bst4CI ACNGT 3 cut(s) 633, 715, 902
Bst6I CTCTTC 1 cut(s) 139
BstAUI TGTACA 1 cut(s) 649
BstBAI YACGTR 1 cut(s) 869
BstBI TTCGAA 1 cut(s) 845
BstDEI CTNAG 5 cut(s) 10, 58, 142, 459, 731
BstDSI CCRYGG 1 cut(s) 510
BstF5I GGATG 3 cut(s) 388, 397, 667
BstFNI CGCG 3 cut(s) 266, 512, 591
BstHHI GCGC 1 cut(s) 591
BstKTI GATC 4 cut(s) 9, 27, 580, 730
BstMAI GTCTC 4 cut(s) 140, 205, 324, 630
BstMBI GATC 4 cut(s) 6, 24, 577, 727
BstMWI GCNNNNNNNGC 5 cut(s) 107, 340, 521, 745, 779
BstNI CCWGG 2 cut(s) 642, 947
BstSCI CCNGG 2 cut(s) 640, 945
BstSFI CTRYAG 1 cut(s) 1029
BstSLI GKGCMC 1 cut(s) 345
BstUI CGCG 3 cut(s) 266, 512, 591
BstV1I GCAGC 2 cut(s) 501, 757
BstV2I GAAGAC 2 cut(s) 834, 903
BstX2I RGATCY 2 cut(s) 24, 727
BstYI RGATCY 2 cut(s) 24, 727
BsuI GTATCC 1 cut(s) 914
BsuRI GGCC 2 cut(s) 739, 1048
BtgI CCRYGG 1 cut(s) 510
BtsCI GGATG 3 cut(s) 388, 397, 667
BtsIMutI CAGTG 1 cut(s) 575
BveI ACCTGC 1 cut(s) 764
CfoI GCGC 1 cut(s) 591
Cfr10I RCCGGY 2 cut(s) 471, 984
Cfr13I GGNCC 3 cut(s) 91, 310, 365
Cfr42I CCGCGG 1 cut(s) 513
CseI GACGC 1 cut(s) 306
Csp6I GTAC 4 cut(s) 49, 295, 650, 823
CviAII CATG 3 cut(s) 723, 795, 1050
CviQI GTAC 4 cut(s) 49, 295, 650, 823
DdeI CTNAG 5 cut(s) 10, 58, 142, 459, 731
DpnI GATC 4 cut(s) 8, 26, 579, 729
DpnII GATC 4 cut(s) 6, 24, 577, 727
DriI GACNNNNNGTC 1 cut(s) 316
EaeI YGGCCR 1 cut(s) 737
Eam1104I CTCTTC 1 cut(s) 139
Eam1105I GACNNNNNGTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 139
EciI GGCGGA 2 cut(s) 73, 609
Ecl136II GAGCTC 1 cut(s) 943
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 945
Eco31I GGTCTC 3 cut(s) 140, 205, 630
Eco32I GATATC 1 cut(s) 664
Eco47I GGWCC 3 cut(s) 91, 310, 365
Eco53kI GAGCTC 1 cut(s) 943
EcoICRI GAGCTC 1 cut(s) 943
EcoO109I RGGNCCY 1 cut(s) 365
EcoRII CCWGG 2 cut(s) 640, 945
EcoRV GATATC 1 cut(s) 664
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 945
ErhI CCWWGG 1 cut(s) 87
Esp3I CGTCTC 1 cut(s) 324
FaeI CATG 3 cut(s) 726, 798, 1053
FaqI GGGAC 3 cut(s) 378, 467, 724
FatI CATG 3 cut(s) 722, 794, 1049
FauI CCCGC 2 cut(s) 81, 192
Fnu4HI GCNGC 7 cut(s) 261, 264, 490, 493, 513, 592, 746
FokI GGATG 3 cut(s) 395, 404, 654
FriOI GRGCYC 1 cut(s) 945
Fsp4HI GCNGC 7 cut(s) 261, 264, 490, 493, 513, 592, 746
GlaI GCGC 1 cut(s) 590
GluI GCNGC 7 cut(s) 261, 264, 490, 493, 513, 592, 746
GsaI CCCAGC 1 cut(s) 173
GsuI CTGGAG 3 cut(s) 551, 624, 717
HaeIII GGCC 2 cut(s) 739, 1048
HapII CCGG 4 cut(s) 64, 276, 472, 985
HgaI GACGC 1 cut(s) 306
HhaI GCGC 1 cut(s) 591
Hin1II CATG 3 cut(s) 726, 798, 1053
Hin6I GCGC 1 cut(s) 589
HinP1I GCGC 1 cut(s) 589
HincII GTYRAC 1 cut(s) 636
HindII GTYRAC 1 cut(s) 636
HinfI GANTC 8 cut(s) 33, 56, 119, 125, 131, 302, 455, 1025
HpaII CCGG 4 cut(s) 64, 276, 472, 985
HphI GGTGA 3 cut(s) 783, 788, 938
Hpy166II GTNNAC 2 cut(s) 507, 636
Hpy188III TCNNGA 3 cut(s) 210, 675, 1039
Hpy8I GTNNAC 2 cut(s) 507, 636
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 3 cut(s) 681, 764, 1005
HpyCH4III ACNGT 3 cut(s) 633, 715, 902
HpyCH4IV ACGT 1 cut(s) 868
HpyCH4V TGCA 4 cut(s) 110, 722, 836, 962
HpyF10VI GCNNNNNNNGC 5 cut(s) 107, 340, 521, 745, 779
HpyF3I CTNAG 5 cut(s) 10, 58, 142, 459, 731
HpySE526I ACGT 1 cut(s) 868
Hsp92II CATG 3 cut(s) 726, 798, 1053
HspAI GCGC 1 cut(s) 589
KflI GGGWCCC 1 cut(s) 365
KspI CCGCGG 1 cut(s) 513
Kzo9I GATC 4 cut(s) 6, 24, 577, 727
LmnI GCTCC 5 cut(s) 75, 284, 331, 596, 948
Lsp1109I GCAGC 2 cut(s) 501, 757
LweI GCATC 1 cut(s) 533
MaeII ACGT 1 cut(s) 868
MaeIII GTNAC 1 cut(s) 354
MalI GATC 4 cut(s) 8, 26, 579, 729
MbiI CCGCTC 1 cut(s) 599
MboI GATC 4 cut(s) 6, 24, 577, 727
MboII GAAGA 5 cut(s) 126, 434, 834, 855, 908
MfeI CAATTG 1 cut(s) 657
MflI RGATCY 2 cut(s) 24, 727
MhlI GDGCHC 2 cut(s) 345, 945
MlsI TGGCCA 1 cut(s) 739
MluCI AATT 4 cut(s) 213, 436, 608, 657
MluNI TGGCCA 1 cut(s) 739
MlyI GAGTC 5 cut(s) 27, 50, 113, 125, 449
MmeI TCCRAC 2 cut(s) 153, 381
Mox20I TGGCCA 1 cut(s) 739
MroXI GAANNNNTTC 1 cut(s) 859
MscI TGGCCA 1 cut(s) 739
MseI TTAA 1 cut(s) 612
Msp20I TGGCCA 1 cut(s) 739
MspA1I CMGCKG 2 cut(s) 492, 512
MspI CCGG 4 cut(s) 64, 276, 472, 985
MspR9I CCNGG 2 cut(s) 642, 947
MunI CAATTG 1 cut(s) 657
MvaI CCWGG 2 cut(s) 642, 947
MvnI CGCG 3 cut(s) 266, 512, 591
MwoI GCNNNNNNNGC 5 cut(s) 107, 340, 521, 745, 779
NdeII GATC 4 cut(s) 6, 24, 577, 727
NlaIII CATG 3 cut(s) 726, 798, 1053
NlaIV GGNNCC 7 cut(s) 71, 280, 312, 342, 366, 367, 933
NspV TTCGAA 1 cut(s) 845
PaqCI CACCTGC 1 cut(s) 764
PdmI GAANNNNTTC 1 cut(s) 859
PfeI GAWTC 3 cut(s) 125, 302, 1025
PflFI GACNNNGTC 1 cut(s) 59
PkrI GCNGC 7 cut(s) 262, 265, 491, 494, 514, 593, 747
PleI GAGTC 5 cut(s) 27, 50, 113, 125, 449
PpsI GAGTC 5 cut(s) 27, 50, 113, 125, 449
Ppu21I YACGTR 1 cut(s) 869
PpuMI RGGWCCY 1 cut(s) 365
Psp124BI GAGCTC 1 cut(s) 945
Psp5II RGGWCCY 1 cut(s) 365
Psp6I CCWGG 2 cut(s) 640, 945
PspFI CCCAGC 1 cut(s) 169
PspGI CCWGG 2 cut(s) 640, 945
PspN4I GGNNCC 7 cut(s) 71, 280, 312, 342, 366, 367, 933
PspPI GGNCC 3 cut(s) 91, 310, 365
PspPPI RGGWCCY 1 cut(s) 365
PsuI RGATCY 2 cut(s) 24, 727
PsyI GACNNNGTC 1 cut(s) 59
RsaI GTAC 4 cut(s) 50, 296, 651, 824
RsaNI GTAC 4 cut(s) 49, 295, 650, 823
SacI GAGCTC 1 cut(s) 945
SacII CCGCGG 1 cut(s) 513
SaqAI TTAA 1 cut(s) 612
SatI GCNGC 7 cut(s) 261, 264, 490, 493, 513, 592, 746
Sau3AI GATC 4 cut(s) 6, 24, 577, 727
Sau96I GGNCC 3 cut(s) 91, 310, 365
SchI GAGTC 5 cut(s) 27, 50, 113, 125, 449
ScrFI CCNGG 2 cut(s) 642, 947
SduI GDGCHC 2 cut(s) 345, 945
SfaNI GCATC 1 cut(s) 533
SfcI CTRYAG 1 cut(s) 1029
Sfr303I CCGCGG 1 cut(s) 513
SfuI TTCGAA 1 cut(s) 845
SgrBI CCGCGG 1 cut(s) 513
SinI GGWCC 3 cut(s) 91, 310, 365
Sse9I AATT 4 cut(s) 213, 436, 608, 657
SstI GAGCTC 1 cut(s) 945
StyD4I CCNGG 2 cut(s) 640, 945
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 3 cut(s) 633, 715, 902
TaiI ACGT 1 cut(s) 871
TaqI TCGA 4 cut(s) 117, 483, 845, 939
TasI AATT 4 cut(s) 213, 436, 608, 657
TatI WGTACW 2 cut(s) 48, 649
TauI GCSGC 5 cut(s) 263, 266, 495, 515, 594
TfiI GAWTC 3 cut(s) 125, 302, 1025
Tru1I TTAA 1 cut(s) 612
Tru9I TTAA 1 cut(s) 612
TscAI CASTG 1 cut(s) 575
TseI GCWGC 2 cut(s) 489, 745
TspRI CASTG 1 cut(s) 575
Tth111I GACNNNGTC 1 cut(s) 59
VpaK11BI GGWCC 3 cut(s) 91, 310, 365
XapI RAATTY 1 cut(s) 213
XcmI CCANNNNNNNNNTGG 2 cut(s) 376, 943
XmnI GAANNNNTTC 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.