pycom10g23530

Nucleoporin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011206
Physical Location & Seq
Reverse (-)
25644048 .. 25645635
1588 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g23530.3

Sequence Viewer

Length: 747 bp
ATGGACCCTGCGTATTTCCAATCGGAGCTTTCGGTGCCCGAAAAGGAGTTACACGAAGGACCAATTTCTTGTGTGGATGTTATGGATGGTGGGTCGGAGTGTGTAAGTGTTGGGGAAGATGGGAGGGTCAATTTGGTAAGTTTAGGGGACTCTGAGTTGAGCTACCGGCGAGTTTTCGACAGCAGCGGCTTGGTTTCGTTTACCGCGGCGAAATGGGCATCTCCGAGTGAGTTTGCTACTGGTGGATATGGGTTTAGCCTCCAGTGGTGGGATCAGAGGAAGCGCGGCGGAGCGGTTTCTCAATTTAAGGGTGGCTGGTCTCAACCGTCAACTCCAGGGATTGTACATTCAATTGATATCCATCCTTCGAGAAAGCACACTTGTTTGGCTGGAGGTTCTTTAGGGACTGTATTTGCGTGGGATCTTAGGTGGCCACAGCAGCCAATCCTTCTTTCTGGGGTTGGGGCAGGTGAAGCAACCCATTCACCATGCGAAAGTGAGGTTTGGGAGGTTCAGTACGACCACTATGCAAAGTCTTCGAATGTGGGAAATGTTTCTTCACGTATCTTACCTACTATGATATGTTCAGAAGATGGTATCCTTGCTGTTGTTGAACAAGGTGAGGAACCCATCGAGCTCCTGGCAGAACCTTGTGCAATCAACAGCTTTGACATAGACCGGCAATATCCTTCTGATGTTATATGTAGTCTGGAGTGGGAATCTATAGCCATCTTGACAAGGCCATGA

Protein Analysis

249

Amino Acids

26.96

Weight (kDa)

4.55

Isoelectric Point (pI)

57.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011206)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30840
fragaria_vesca FvH4_4g09770 FvH4_4g09770 FvH4_4g09770
malus_domestica MD10G1281400.v1.1
prunus_persica Prupe.1G102000_v2.0.a1 Prupe.1G102000_v2.0.a1 Prupe.1G102000_v2.0.a1
pyrus_communis pycom10g23530
rosa_chinensis RchiOBHm_Chr4g0405491
rosa_laevigata RLG00000008840
rosa_multiflora Rmu_co8147422.1_g000001
rosa_roxburghii Rroxscaffold_5G00349930
rosa_rugosa Rorug04G0060500 Rorug04G0060600
rosa_samantha Rh4AG132200 Rh4BG127000 Rh4CG139700 Rh4DG126300
rosa_wichuraiana Rw4G010700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 458
Acc36I ACCTGC 1 cut(s) 458
AccB1I GGYRCC 1 cut(s) 34
AccBSI CCGCTC 1 cut(s) 293
AccII CGCG 2 cut(s) 206, 285
AciI CCGC 6 cut(s) 186, 204, 206, 285, 288, 293
AclWI GGATC 2 cut(s) 279, 429
AcoI YGGCCR 1 cut(s) 431
AfaI GTAC 2 cut(s) 345, 518
AfiI CCNNNNNNNGG 1 cut(s) 268
AgsI TTSAA 2 cut(s) 351, 614
AjnI CCWGG 2 cut(s) 334, 639
AjuI GAANNNNNNNTTGG 2 cut(s) 436, 468
AluBI AGCT 4 cut(s) 28, 162, 637, 666
AluI AGCT 4 cut(s) 28, 162, 637, 666
Alw21I GWGCWC 1 cut(s) 639
Alw26I GTCTC 1 cut(s) 324
AlwI GGATC 2 cut(s) 279, 429
AoxI GGCC 2 cut(s) 431, 740
ApeKI GCWGC 2 cut(s) 183, 439
Asp700I GAANNNNTTC 1 cut(s) 553
AspLEI GCGC 1 cut(s) 285
AspS9I GGNCC 2 cut(s) 4, 59
AsuHPI GGTGA 3 cut(s) 477, 482, 632
AsuII TTCGAA 1 cut(s) 539
AvaII GGWCC 2 cut(s) 4, 59
BaeGI GKGCMC 1 cut(s) 39
BalI TGGCCA 1 cut(s) 433
BanI GGYRCC 1 cut(s) 34
BanII GRGCYC 1 cut(s) 639
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 1 cut(s) 639
BbvI GCAGC 2 cut(s) 195, 451
BccI CCATC 6 cut(s) 80, 113, 369, 587, 638, 737
BcgI CGANNNNNNTGC 4 cut(s) 509, 519, 543, 553
BciT130I CCWGG 2 cut(s) 336, 641
BciVI GTATCC 1 cut(s) 608
BcoDI GTCTC 1 cut(s) 324
BfmI CTRYAG 1 cut(s) 723
BfuAI ACCTGC 1 cut(s) 458
BfuI GTATCC 1 cut(s) 608
BisI GCNGC 5 cut(s) 184, 187, 207, 286, 440
BlsI GCNGC 5 cut(s) 185, 188, 208, 287, 441
Bme1390I CCNGG 2 cut(s) 336, 641
Bme18I GGWCC 2 cut(s) 4, 59
BmgT120I GGNCC 2 cut(s) 4, 59
BmiI GGNNCC 3 cut(s) 6, 36, 627
BmrFI CCNGG 2 cut(s) 336, 641
BmsI GCATC 1 cut(s) 227
BpiI GAAGAC 1 cut(s) 528
BpmI CTGGAG 4 cut(s) 245, 318, 411, 731
Bpu14I TTCGAA 1 cut(s) 539
BsaAI YACGTR 1 cut(s) 563
BsaBI GATNNNNATC 1 cut(s) 360
BsaI GGTCTC 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 204, 335
BsaXI ACNNNNNCTCC 2 cut(s) 500, 530
Bsc4I CCNNNNNNNGG 1 cut(s) 268
Bse118I RCCGGY 2 cut(s) 165, 678
Bse1I ACTGG 2 cut(s) 244, 262
Bse8I GATNNNNATC 1 cut(s) 360
BseBI CCWGG 2 cut(s) 336, 641
BseDI CCNNGG 2 cut(s) 204, 335
BseGI GGATG 3 cut(s) 82, 91, 361
BseJI GATNNNNATC 1 cut(s) 360
BseLI CCNNNNNNNGG 1 cut(s) 268
BseMII CTCAG 1 cut(s) 144
BseNI ACTGG 2 cut(s) 244, 262
BseSI GKGCMC 1 cut(s) 39
BseXI GCAGC 2 cut(s) 195, 451
Bsh1236I CGCG 2 cut(s) 206, 285
BshFI GGCC 2 cut(s) 433, 742
BshNI GGYRCC 1 cut(s) 34
BsiHKAI GWGCWC 1 cut(s) 639
BsiSI CCGG 2 cut(s) 166, 679
BslFI GGGAC 2 cut(s) 161, 418
BslI CCNNNNNNNGG 1 cut(s) 268
BsmAI GTCTC 1 cut(s) 324
BsmFI GGGAC 2 cut(s) 161, 418
BsnI GGCC 2 cut(s) 433, 742
Bso31I GGTCTC 1 cut(s) 324
Bsp119I TTCGAA 1 cut(s) 539
Bsp1286I GDGCHC 2 cut(s) 39, 639
Bsp1407I TGTACA 1 cut(s) 343
Bsp143I GATC 2 cut(s) 271, 421
BspACI CCGC 6 cut(s) 186, 204, 206, 285, 288, 293
BspANI GGCC 2 cut(s) 433, 742
BspCNI CTCAG 1 cut(s) 145
BspFNI CGCG 2 cut(s) 206, 285
BspLI GGNNCC 3 cut(s) 6, 36, 627
BspMI ACCTGC 1 cut(s) 458
BspPI GGATC 2 cut(s) 279, 429
BspT104I TTCGAA 1 cut(s) 539
BspT107I GGYRCC 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 324
BsrBI CCGCTC 1 cut(s) 293
BsrFI RCCGGY 2 cut(s) 165, 678
BsrGI TGTACA 1 cut(s) 343
BsrI ACTGG 2 cut(s) 244, 262
BssAI RCCGGY 2 cut(s) 165, 678
BssECI CCNNGG 2 cut(s) 204, 335
BssMI GATC 2 cut(s) 271, 421
Bst2UI CCWGG 2 cut(s) 336, 641
Bst4CI ACNGT 2 cut(s) 327, 409
BstAUI TGTACA 1 cut(s) 343
BstBAI YACGTR 1 cut(s) 563
BstBI TTCGAA 1 cut(s) 539
BstDEI CTNAG 2 cut(s) 153, 425
BstDSI CCRYGG 1 cut(s) 204
BstF5I GGATG 3 cut(s) 82, 91, 361
BstFNI CGCG 2 cut(s) 206, 285
BstHHI GCGC 1 cut(s) 285
BstKTI GATC 2 cut(s) 274, 424
BstMAI GTCTC 1 cut(s) 324
BstMBI GATC 2 cut(s) 271, 421
BstMWI GCNNNNNNNGC 4 cut(s) 34, 215, 439, 473
BstNI CCWGG 2 cut(s) 336, 641
BstSCI CCNGG 2 cut(s) 334, 639
BstSFI CTRYAG 1 cut(s) 723
BstSLI GKGCMC 1 cut(s) 39
BstUI CGCG 2 cut(s) 206, 285
BstV1I GCAGC 2 cut(s) 195, 451
BstV2I GAAGAC 1 cut(s) 528
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
BsuI GTATCC 1 cut(s) 608
BsuRI GGCC 2 cut(s) 433, 742
BtgI CCRYGG 1 cut(s) 204
BtsCI GGATG 3 cut(s) 82, 91, 361
BtsIMutI CAGTG 1 cut(s) 269
BveI ACCTGC 1 cut(s) 458
CfoI GCGC 1 cut(s) 285
Cfr10I RCCGGY 2 cut(s) 165, 678
Cfr13I GGNCC 2 cut(s) 4, 59
Cfr42I CCGCGG 1 cut(s) 207
Csp6I GTAC 2 cut(s) 344, 517
CviAII CATG 2 cut(s) 489, 744
CviQI GTAC 2 cut(s) 344, 517
DdeI CTNAG 2 cut(s) 153, 425
DpnI GATC 2 cut(s) 273, 423
DpnII GATC 2 cut(s) 271, 421
EaeI YGGCCR 1 cut(s) 431
EciI GGCGGA 1 cut(s) 303
Ecl136II GAGCTC 1 cut(s) 637
Eco24I GRGCYC 1 cut(s) 639
Eco31I GGTCTC 1 cut(s) 324
Eco32I GATATC 1 cut(s) 358
Eco47I GGWCC 2 cut(s) 4, 59
Eco53kI GAGCTC 1 cut(s) 637
EcoICRI GAGCTC 1 cut(s) 637
EcoRII CCWGG 2 cut(s) 334, 639
EcoRV GATATC 1 cut(s) 358
EcoT38I GRGCYC 1 cut(s) 639
FaeI CATG 2 cut(s) 492, 747
FaqI GGGAC 2 cut(s) 161, 418
FatI CATG 2 cut(s) 488, 743
Fnu4HI GCNGC 5 cut(s) 184, 187, 207, 286, 440
FokI GGATG 3 cut(s) 89, 98, 348
FriOI GRGCYC 1 cut(s) 639
Fsp4HI GCNGC 5 cut(s) 184, 187, 207, 286, 440
GlaI GCGC 1 cut(s) 284
GluI GCNGC 5 cut(s) 184, 187, 207, 286, 440
GsuI CTGGAG 4 cut(s) 245, 318, 411, 731
HaeIII GGCC 2 cut(s) 433, 742
HapII CCGG 2 cut(s) 166, 679
HhaI GCGC 1 cut(s) 285
Hin1II CATG 2 cut(s) 492, 747
Hin6I GCGC 1 cut(s) 283
HinP1I GCGC 1 cut(s) 283
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 2 cut(s) 149, 719
HpaII CCGG 2 cut(s) 166, 679
HphI GGTGA 3 cut(s) 477, 482, 632
Hpy166II GTNNAC 2 cut(s) 201, 330
Hpy188I TCNGA 7 cut(s) 25, 97, 154, 225, 276, 589, 694
Hpy188III TCNNGA 3 cut(s) 369, 710, 733
Hpy8I GTNNAC 2 cut(s) 201, 330
HpyAV CCTTC 4 cut(s) 50, 375, 458, 699
HpyCH4III ACNGT 2 cut(s) 327, 409
HpyCH4IV ACGT 1 cut(s) 562
HpyCH4V TGCA 2 cut(s) 530, 656
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 215, 439, 473
HpyF3I CTNAG 2 cut(s) 153, 425
HpySE526I ACGT 1 cut(s) 562
Hsp92II CATG 2 cut(s) 492, 747
HspAI GCGC 1 cut(s) 283
KspI CCGCGG 1 cut(s) 207
Kzo9I GATC 2 cut(s) 271, 421
LmnI GCTCC 3 cut(s) 25, 290, 642
Lsp1109I GCAGC 2 cut(s) 195, 451
LweI GCATC 1 cut(s) 227
MaeII ACGT 1 cut(s) 562
MaeIII GTNAC 1 cut(s) 48
MalI GATC 2 cut(s) 273, 423
MbiI CCGCTC 1 cut(s) 293
MboI GATC 2 cut(s) 271, 421
MboII GAAGA 4 cut(s) 128, 528, 549, 602
MfeI CAATTG 1 cut(s) 351
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 2 cut(s) 39, 639
MlsI TGGCCA 1 cut(s) 433
MluCI AATT 4 cut(s) 63, 130, 302, 351
MluNI TGGCCA 1 cut(s) 433
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 1 cut(s) 75
MnlI CCTC 7 cut(s) 117, 269, 270, 386, 493, 502, 616
Mox20I TGGCCA 1 cut(s) 433
MroXI GAANNNNTTC 1 cut(s) 553
MscI TGGCCA 1 cut(s) 433
MseI TTAA 1 cut(s) 306
Msp20I TGGCCA 1 cut(s) 433
MspA1I CMGCKG 2 cut(s) 186, 206
MspI CCGG 2 cut(s) 166, 679
MspR9I CCNGG 2 cut(s) 336, 641
MunI CAATTG 1 cut(s) 351
MvaI CCWGG 2 cut(s) 336, 641
MvnI CGCG 2 cut(s) 206, 285
MwoI GCNNNNNNNGC 4 cut(s) 34, 215, 439, 473
NdeII GATC 2 cut(s) 271, 421
NlaIII CATG 2 cut(s) 492, 747
NlaIV GGNNCC 3 cut(s) 6, 36, 627
NspV TTCGAA 1 cut(s) 539
PaqCI CACCTGC 1 cut(s) 458
PdmI GAANNNNTTC 1 cut(s) 553
PfeI GAWTC 1 cut(s) 719
PkrI GCNGC 5 cut(s) 185, 188, 208, 287, 441
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
Ppu21I YACGTR 1 cut(s) 563
Psp124BI GAGCTC 1 cut(s) 639
Psp6I CCWGG 2 cut(s) 334, 639
PspGI CCWGG 2 cut(s) 334, 639
PspN4I GGNNCC 3 cut(s) 6, 36, 627
PspPI GGNCC 2 cut(s) 4, 59
PsuI RGATCY 1 cut(s) 421
RsaI GTAC 2 cut(s) 345, 518
RsaNI GTAC 2 cut(s) 344, 517
SacI GAGCTC 1 cut(s) 639
SacII CCGCGG 1 cut(s) 207
SaqAI TTAA 1 cut(s) 306
SatI GCNGC 5 cut(s) 184, 187, 207, 286, 440
Sau3AI GATC 2 cut(s) 271, 421
Sau96I GGNCC 2 cut(s) 4, 59
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 2 cut(s) 336, 641
SduI GDGCHC 2 cut(s) 39, 639
SfaNI GCATC 1 cut(s) 227
SfcI CTRYAG 1 cut(s) 723
Sfr303I CCGCGG 1 cut(s) 207
SfuI TTCGAA 1 cut(s) 539
SgrBI CCGCGG 1 cut(s) 207
SinI GGWCC 2 cut(s) 4, 59
Sse9I AATT 4 cut(s) 63, 130, 302, 351
SsiI CCGC 6 cut(s) 186, 204, 206, 285, 288, 293
SstI GAGCTC 1 cut(s) 639
StyD4I CCNGG 2 cut(s) 334, 639
TaaI ACNGT 2 cut(s) 327, 409
TaiI ACGT 1 cut(s) 565
TaqI TCGA 4 cut(s) 177, 368, 539, 633
TasI AATT 4 cut(s) 63, 130, 302, 351
TatI WGTACW 1 cut(s) 343
TauI GCSGC 3 cut(s) 189, 209, 288
TfiI GAWTC 1 cut(s) 719
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TscAI CASTG 1 cut(s) 269
TseI GCWGC 2 cut(s) 183, 439
TspRI CASTG 1 cut(s) 269
VpaK11BI GGWCC 2 cut(s) 4, 59
XcmI CCANNNNNNNNNTGG 1 cut(s) 637
XmnI GAANNNNTTC 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.