MD11G1001800.v1.1

NusB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010441
Physical Location & Seq
Forward (+)
183791 .. 186987
3197 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1001800.v1.1.491

Sequence Viewer

Length: 897 bp
ATGGAGGGAAGCTCGCTTCTCAGTCTCGGCTGCTCCTCTTCTTCTCCCCATTTAAAACCTCATTTCTCTTTCTCCTCCTCTTCTTCCTTGCTCTTCGGAAAATCCCACCTTCTGGGTTTAATCCCCAGACCCTTACTCGCCCACAGCACCCTATCACTCCCTTCCCCTCGAACCGCCCTCCGCACTTCGTCCTTCCCTCTCGACCAAGCTCTCGAAAAGCCCAGCAATGGCTCAGCAGATACGTTGCCCAAGATTGATAAGAGTGGCAGATTCTGCAGCCCCAGAGCCGCCAGAGAGCTCGCTCTGTCGATTGTTTATGCTTCTTGTTTAGAGGGTTCTGACCCAATACGTTTATTTGAAAAGCGAATAAATGTCCGTCGAGAACCCGGGTATGAATTTGACAGGGCATCATTACTTGAATATAATCCCATGAGCTTCGGAGGACCCCCTGTCACAGTTGAAACTGTTGAGGAAGCAGATGAGCTCCTGCGCAAAGATGAAAAGGAATCTGCAATTGCAGAAGTTCTTGCAGCTCCTCCAAAATTGGTATACAGCAAACTGATTTTGCGATTTACAAGGAAACTTTTGGTTGCAGTCATGGATAAATGGGACAGTCATGTGCTCGTTATTGACAACGTTGCCCCTCCAAATTGGAAGGATGAGCCAGCAGGCAGAATATTGGAGCTTTGCATCCTCCACTTGGCAATGTCAGAAATAACAGTGCTCGAAACAAGACACCCGATTGTCATTAACGAGGCCGTAGATCTTGCTAAACGGTTCTGTGATGGATCAGCACCCCGTATTATCAATGGTTGTCTTAGAACATTTGTCAAACAAATTGAAGAAGCTGGTGGTCTAACTCGGGCTCTGGGGTATAAACAGAATGTGGTCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NusB

Protein Analysis

299

Amino Acids

32.74

Weight (kDa)

7.65

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NusB PF01029 217 - 278 4.6e-09 NusB family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26370 AT4G26370 AT4G26370
fragaria_vesca FvH4_3g45910 FvH4_3g45910 FvH4_3g45910
malus_domestica MD11G1001800.v1.1
prunus_persica Prupe.6G001800_v2.0.a1
pyrus_communis pycom11g00180
rosa_chinensis RchiOBHm_Chr5g0084001
rosa_laevigata RLG00000037071
rosa_multiflora Rmu_sc0001137.1_g000001 Rmu_ssc0000372.1_g000077
rosa_roxburghii Rroxscaffold_1G00000650
rosa_rugosa Rorug05G0488200 Rorug05G0488300
rosa_samantha Rh5AG540900 Rh5BG566200 Rh5CG588200 Rh5DG574500
rosa_wichuraiana Rw5G050300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 491
AccB7I CCANNNNNTGG 1 cut(s) 112
AccI GTMKAC 1 cut(s) 549
AciI CCGC 3 cut(s) 174, 181, 288
AclI AACGTT 1 cut(s) 636
AclWI GGATC 1 cut(s) 796
AcsI RAATTY 1 cut(s) 395
AfiI CCNNNNNNNGG 3 cut(s) 112, 227, 700
AgsI TTSAA 4 cut(s) 359, 419, 461, 842
AhdI GACNNNNNGTC 1 cut(s) 449
AluBI AGCT 8 cut(s) 12, 209, 298, 435, 484, 533, 685, 848
AluI AGCT 8 cut(s) 12, 209, 298, 435, 484, 533, 685, 848
Alw21I GWGCWC 4 cut(s) 300, 486, 624, 726
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 1 cut(s) 796
AlwNI CAGNNNCTG 1 cut(s) 273
Ama87I CYCGRG 2 cut(s) 386, 861
AoxI GGCC 1 cut(s) 756
ApeKI GCWGC 3 cut(s) 30, 276, 530
ApoI RAATTY 1 cut(s) 395
AspLEI GCGC 1 cut(s) 492
AspS9I GGNCC 1 cut(s) 443
AsuC2I CCSGG 2 cut(s) 387, 388
AvaI CYCGRG 2 cut(s) 386, 861
AvaII GGWCC 1 cut(s) 443
BanII GRGCYC 3 cut(s) 300, 486, 868
Bbv12I GWGCWC 4 cut(s) 300, 486, 624, 726
BbvI GCAGC 3 cut(s) 17, 288, 542
BccI CCATC 1 cut(s) 779
BceAI ACGGC 1 cut(s) 743
BcnI CCSGG 2 cut(s) 387, 388
BcoDI GTCTC 1 cut(s) 29
BfmI CTRYAG 1 cut(s) 274
BglII AGATCT 1 cut(s) 763
BisI GCNGC 4 cut(s) 31, 277, 288, 531
BlpI GCTNAGC 1 cut(s) 232
BlsI GCNGC 4 cut(s) 32, 278, 289, 532
Bme1390I CCNGG 2 cut(s) 387, 388
Bme18I GGWCC 1 cut(s) 443
BmeRI GACNNNNNGTC 1 cut(s) 449
BmeT110I CYCGRG 2 cut(s) 386, 861
BmgT120I GGNCC 1 cut(s) 443
BmiI GGNNCC 1 cut(s) 445
BmrFI CCNGG 2 cut(s) 387, 388
BmsI GCATC 2 cut(s) 416, 699
BplI GAGNNNNNCTC 1 cut(s) 28
Bpu1102I GCTNAGC 1 cut(s) 232
BpuMI CCSGG 2 cut(s) 387, 388
BsaJI CCNNGG 1 cut(s) 386
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 227, 700
Bse3DI GCAATG 2 cut(s) 232, 711
BseDI CCNNGG 1 cut(s) 386
BseGI GGATG 2 cut(s) 664, 690
BseLI CCNNNNNNNGG 3 cut(s) 112, 227, 700
BseMI GCAATG 2 cut(s) 232, 711
BseMII CTCAG 2 cut(s) 34, 246
BseRI GAGGAG 4 cut(s) 25, 64, 67, 525
BseXI GCAGC 3 cut(s) 17, 288, 542
BseYI CCCAGC 1 cut(s) 221
BshFI GGCC 1 cut(s) 758
BsiHKAI GWGCWC 4 cut(s) 300, 486, 624, 726
BsiHKCI CYCGRG 2 cut(s) 386, 861
BsiSI CCGG 1 cut(s) 387
BslFI GGGAC 1 cut(s) 623
BslI CCNNNNNNNGG 3 cut(s) 112, 227, 700
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 623
BsnI GGCC 1 cut(s) 758
BsoBI CYCGRG 2 cut(s) 386, 861
Bsp1286I GDGCHC 5 cut(s) 300, 486, 624, 726, 868
Bsp143I GATC 2 cut(s) 763, 788
Bsp1720I GCTNAGC 1 cut(s) 232
BspACI CCGC 3 cut(s) 174, 181, 288
BspANI GGCC 1 cut(s) 758
BspCNI CTCAG 2 cut(s) 33, 245
BspLI GGNNCC 1 cut(s) 445
BspMAI CTGCAG 1 cut(s) 278
BspPI GGATC 1 cut(s) 796
BspQI GCTCTTC 1 cut(s) 98
BsrDI GCAATG 2 cut(s) 232, 711
BssECI CCNNGG 1 cut(s) 386
BssMI GATC 2 cut(s) 763, 788
BssNAI GTATAC 1 cut(s) 550
Bst1107I GTATAC 1 cut(s) 550
Bst4CI ACNGT 5 cut(s) 457, 466, 614, 721, 777
Bst6I CTCTTC 3 cut(s) 43, 85, 98
BstAPI GCANNNNNTGC 1 cut(s) 273
BstC8I GCNNGC 4 cut(s) 14, 300, 666, 670
BstDEI CTNAG 3 cut(s) 20, 232, 818
BstF5I GGATG 2 cut(s) 664, 690
BstHHI GCGC 1 cut(s) 492
BstKTI GATC 2 cut(s) 766, 791
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 2 cut(s) 763, 788
BstMWI GCNNNNNNNGC 1 cut(s) 273
BstSCI CCNGG 2 cut(s) 385, 386
BstSFI CTRYAG 1 cut(s) 274
BstV1I GCAGC 3 cut(s) 17, 288, 542
BstX2I RGATCY 1 cut(s) 763
BstYI RGATCY 1 cut(s) 763
BstZ17I GTATAC 1 cut(s) 550
BsuRI GGCC 1 cut(s) 758
BtsCI GGATG 2 cut(s) 664, 690
BtsIMutI CAGTG 1 cut(s) 726
Cac8I GCNNGC 4 cut(s) 14, 300, 666, 670
CaiI CAGNNNCTG 1 cut(s) 273
CfoI GCGC 1 cut(s) 492
Cfr13I GGNCC 1 cut(s) 443
Cfr9I CCCGGG 1 cut(s) 386
CviAII CATG 3 cut(s) 430, 598, 617
DdeI CTNAG 3 cut(s) 20, 232, 818
DpnI GATC 2 cut(s) 765, 790
DpnII GATC 2 cut(s) 763, 788
DraI TTTAAA 1 cut(s) 54
DriI GACNNNNNGTC 1 cut(s) 449
Eam1104I CTCTTC 3 cut(s) 43, 85, 98
Eam1105I GACNNNNNGTC 1 cut(s) 449
EarI CTCTTC 3 cut(s) 43, 85, 98
Ecl136II GAGCTC 2 cut(s) 298, 484
Eco24I GRGCYC 3 cut(s) 300, 486, 868
Eco47I GGWCC 1 cut(s) 443
Eco53kI GAGCTC 2 cut(s) 298, 484
Eco88I CYCGRG 2 cut(s) 386, 861
EcoICRI GAGCTC 2 cut(s) 298, 484
EcoO109I RGGNCCY 1 cut(s) 443
EcoT38I GRGCYC 3 cut(s) 300, 486, 868
FaeI CATG 3 cut(s) 433, 601, 620
FaiI YATR 8 cut(s) 318, 393, 423, 431, 550, 599, 618, 876
FaqI GGGAC 1 cut(s) 623
FatI CATG 3 cut(s) 429, 597, 616
FblI GTMKAC 1 cut(s) 549
Fnu4HI GCNGC 4 cut(s) 31, 277, 288, 531
FokI GGATG 2 cut(s) 671, 677
FriOI GRGCYC 3 cut(s) 300, 486, 868
Fsp4HI GCNGC 4 cut(s) 31, 277, 288, 531
FspI TGCGCA 1 cut(s) 491
GlaI GCGC 1 cut(s) 491
GluI GCNGC 4 cut(s) 31, 277, 288, 531
GsaI CCCAGC 1 cut(s) 225
HaeIII GGCC 1 cut(s) 758
HapII CCGG 1 cut(s) 387
HhaI GCGC 1 cut(s) 492
Hin1II CATG 3 cut(s) 433, 601, 620
Hin6I GCGC 1 cut(s) 490
HinP1I GCGC 1 cut(s) 490
HinfI GANTC 2 cut(s) 270, 506
HpaII CCGG 1 cut(s) 387
Hpy166II GTNNAC 1 cut(s) 550
Hpy188I TCNGA 4 cut(s) 98, 340, 440, 712
Hpy188III TCNNGA 3 cut(s) 200, 212, 380
Hpy8I GTNNAC 1 cut(s) 550
Hpy99I CGWCG 1 cut(s) 381
HpyAV CCTTC 4 cut(s) 119, 171, 202, 649
HpyCH4III ACNGT 5 cut(s) 457, 466, 614, 721, 777
HpyCH4IV ACGT 3 cut(s) 242, 349, 636
HpyCH4V TGCA 6 cut(s) 276, 512, 518, 530, 593, 690
HpyF10VI GCNNNNNNNGC 1 cut(s) 273
HpyF3I CTNAG 3 cut(s) 20, 232, 818
HpySE526I ACGT 3 cut(s) 242, 349, 636
Hsp92II CATG 3 cut(s) 433, 601, 620
HspAI GCGC 1 cut(s) 490
Kzo9I GATC 2 cut(s) 763, 788
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 4 cut(s) 38, 489, 538, 682
Lsp1109I GCAGC 3 cut(s) 17, 288, 542
LweI GCATC 2 cut(s) 416, 699
MaeII ACGT 3 cut(s) 242, 349, 636
MaeIII GTNAC 2 cut(s) 451, 889
MalI GATC 2 cut(s) 765, 790
MboI GATC 2 cut(s) 763, 788
MboII GAAGA 6 cut(s) 30, 33, 72, 75, 85, 854
MfeI CAATTG 1 cut(s) 513
MflI RGATCY 1 cut(s) 763
MhlI GDGCHC 5 cut(s) 300, 486, 624, 726, 868
MluCI AATT 5 cut(s) 395, 513, 542, 649, 837
MseI TTAA 3 cut(s) 53, 119, 750
MspI CCGG 1 cut(s) 387
MspR9I CCNGG 2 cut(s) 387, 388
MunI CAATTG 1 cut(s) 513
MwoI GCNNNNNNNGC 1 cut(s) 273
NciI CCSGG 2 cut(s) 387, 388
NdeII GATC 2 cut(s) 763, 788
NlaIII CATG 3 cut(s) 433, 601, 620
NlaIV GGNNCC 1 cut(s) 445
NmeAIII GCCGAG 1 cut(s) 6
NmuCI GTSAC 2 cut(s) 451, 889
NsbI TGCGCA 1 cut(s) 491
PciSI GCTCTTC 1 cut(s) 98
PfeI GAWTC 2 cut(s) 270, 506
PflMI CCANNNNNTGG 1 cut(s) 112
PkrI GCNGC 4 cut(s) 32, 278, 289, 532
PpuMI RGGWCCY 1 cut(s) 443
Psp124BI GAGCTC 2 cut(s) 300, 486
Psp1406I AACGTT 1 cut(s) 636
Psp5II RGGWCCY 1 cut(s) 443
PspFI CCCAGC 1 cut(s) 221
PspN4I GGNNCC 1 cut(s) 445
PspPI GGNCC 1 cut(s) 443
PspPPI RGGWCCY 1 cut(s) 443
PstI CTGCAG 1 cut(s) 278
PstNI CAGNNNCTG 1 cut(s) 273
PsuI RGATCY 1 cut(s) 763
SacI GAGCTC 2 cut(s) 300, 486
SapI GCTCTTC 1 cut(s) 98
SaqAI TTAA 3 cut(s) 53, 119, 750
SatI GCNGC 4 cut(s) 31, 277, 288, 531
Sau3AI GATC 2 cut(s) 763, 788
Sau96I GGNCC 1 cut(s) 443
ScrFI CCNGG 2 cut(s) 387, 388
SduI GDGCHC 5 cut(s) 300, 486, 624, 726, 868
SfaNI GCATC 2 cut(s) 416, 699
SfcI CTRYAG 1 cut(s) 274
SinI GGWCC 1 cut(s) 443
SmaI CCCGGG 1 cut(s) 388
Sse9I AATT 5 cut(s) 395, 513, 542, 649, 837
SsiI CCGC 3 cut(s) 174, 181, 288
SspI AATATT 1 cut(s) 678
SstI GAGCTC 2 cut(s) 300, 486
StyD4I CCNGG 2 cut(s) 385, 386
TaaI ACNGT 5 cut(s) 457, 466, 614, 721, 777
TaiI ACGT 3 cut(s) 245, 352, 639
TaqI TCGA 6 cut(s) 169, 201, 213, 308, 379, 726
TasI AATT 5 cut(s) 395, 513, 542, 649, 837
TauI GCSGC 1 cut(s) 290
TfiI GAWTC 2 cut(s) 270, 506
Tru1I TTAA 3 cut(s) 53, 119, 750
Tru9I TTAA 3 cut(s) 53, 119, 750
TscAI CASTG 1 cut(s) 726
TseFI GTSAC 2 cut(s) 451, 889
TseI GCWGC 3 cut(s) 30, 276, 530
Tsp45I GTSAC 2 cut(s) 451, 889
TspDTI ATGAA 2 cut(s) 408, 513
TspGWI ACGGA 1 cut(s) 365
TspMI CCCGGG 1 cut(s) 386
TspRI CASTG 1 cut(s) 726
Van91I CCANNNNNTGG 1 cut(s) 112
VpaK11BI GGWCC 1 cut(s) 443
XapI RAATTY 1 cut(s) 395
XmaI CCCGGG 1 cut(s) 386
XmiI GTMKAC 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.