Rmu_ssc0000372.1_g000077

NusB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000372.1
OG0010441
Physical Location & Seq
Reverse (-)
285376 .. 288498
3123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000372.1_g000077.1.cds

Sequence Viewer

Length: 819 bp
atggagggaagctcgcttctcagtctcagctgctcctctgctcttcccaatttaatacccactccttcttactcctctctaagctttactctctcccactcctcatcctcttcttcttctcctttgctatttggtagatcccatctcgtgggtttaatcccaagaccattcactgttcgaacctctctccgcacttccacgttcgctgccgtcgatgaagctctcgaaaagtccaactctgattccagagatatgttgcccaagattgacaagagtggcagattttgcagccccagagccgccagagagctcgctctctccattatttatgcttcctgcttggaaggttccgacccagtacgcttatttgaaaagcgaatgaatgtccgtcgagaacccgggtatgaatttgacaaggcttcattgttggaatataatcccatgagcttcggaggaccccctgtcacagttcaaactgttgaagaagcagatgagctcctgctcaatgacgaaaaggaatctgcaatcgaagcggaagttctagcagctcctccaaagttggtatacagcaaactgattttaagatttgcaaggaaacttttggttgcagttatggataaatgggacagtcatgtgcttgtcattgacagagttgcccctccaaattggaaggctgtagatcttgctaaacgtttctgtgatgggtcagccccccgcacaatcaatggttgtcttagaacattcgtcaatggccttgaagaatctggtttaactccagctttggggaaacagaaagcggtcactatcagtattaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NusB

Protein Analysis

272

Amino Acids

29.69

Weight (kDa)

7.62

Isoelectric Point (pI)

47.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26370 AT4G26370 AT4G26370
fragaria_vesca FvH4_3g45910 FvH4_3g45910 FvH4_3g45910
malus_domestica MD11G1001800.v1.1
prunus_persica Prupe.6G001800_v2.0.a1
pyrus_communis pycom11g00180
rosa_chinensis RchiOBHm_Chr5g0084001
rosa_laevigata RLG00000037071
rosa_multiflora Rmu_sc0001137.1_g000001 Rmu_ssc0000372.1_g000077
rosa_roxburghii Rroxscaffold_1G00000650
rosa_rugosa Rorug05G0488200 Rorug05G0488300
rosa_samantha Rh5AG540900 Rh5BG566200 Rh5CG588200 Rh5DG574500
rosa_wichuraiana Rw5G050300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 148
AccI GTMKAC 1 cut(s) 564
AciI CCGC 5 cut(s) 190, 300, 533, 715, 797
AclI AACGTT 1 cut(s) 691
AclWI GGATC 1 cut(s) 132
AcsI RAATTY 1 cut(s) 407
AfaI GTAC 1 cut(s) 360
AfiI CCNNNNNNNGG 2 cut(s) 148, 782
AgsI TTSAA 4 cut(s) 371, 473, 482, 758
AhdI GACNNNNNGTC 1 cut(s) 461
AluBI AGCT 9 cut(s) 12, 30, 84, 221, 310, 447, 496, 548, 779
AluI AGCT 9 cut(s) 12, 30, 84, 221, 310, 447, 496, 548, 779
Alw21I GWGCWC 2 cut(s) 312, 498
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 1 cut(s) 132
Ama87I CYCGRG 1 cut(s) 398
AoxI GGCC 1 cut(s) 751
ApeKI GCWGC 4 cut(s) 30, 206, 288, 545
ApoI RAATTY 1 cut(s) 407
AspS9I GGNCC 1 cut(s) 455
AsuC2I CCSGG 2 cut(s) 399, 400
AsuII TTCGAA 1 cut(s) 178
AvaI CYCGRG 1 cut(s) 398
AvaII GGWCC 1 cut(s) 455
BanII GRGCYC 2 cut(s) 312, 498
BauI CACGAG 1 cut(s) 146
Bbv12I GWGCWC 2 cut(s) 312, 498
BbvI GCAGC 4 cut(s) 17, 193, 300, 557
BccI CCATC 2 cut(s) 150, 695
BceAI ACGGC 1 cut(s) 194
BcnI CCSGG 2 cut(s) 399, 400
BcoDI GTCTC 1 cut(s) 29
BfaI CTAG 1 cut(s) 542
BfmI CTRYAG 1 cut(s) 675
BglII AGATCT 1 cut(s) 679
BisI GCNGC 5 cut(s) 31, 207, 289, 300, 546
BlsI GCNGC 5 cut(s) 32, 208, 290, 301, 547
Bme1390I CCNGG 2 cut(s) 399, 400
Bme18I GGWCC 1 cut(s) 455
BmeRI GACNNNNNGTC 1 cut(s) 461
BmeT110I CYCGRG 1 cut(s) 398
BmgT120I GGNCC 1 cut(s) 455
BmiI GGNNCC 2 cut(s) 349, 457
BmrFI CCNGG 2 cut(s) 399, 400
BmrI ACTGGG 1 cut(s) 350
BmuI ACTGGG 1 cut(s) 350
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 759
Bpu14I TTCGAA 1 cut(s) 178
BpuMI CCSGG 2 cut(s) 399, 400
BsaJI CCNNGG 1 cut(s) 398
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 782
Bse1I ACTGG 1 cut(s) 356
BseDI CCNNGG 1 cut(s) 398
BseGI GGATG 1 cut(s) 104
BseLI CCNNNNNNNGG 2 cut(s) 148, 782
BseMII CTCAG 2 cut(s) 34, 40
BseNI ACTGG 1 cut(s) 356
BseRI GAGGAG 4 cut(s) 25, 64, 91, 540
BseXI GCAGC 4 cut(s) 17, 193, 300, 557
BshFI GGCC 1 cut(s) 753
BsiHKAI GWGCWC 2 cut(s) 312, 498
BsiHKCI CYCGRG 1 cut(s) 398
BsiSI CCGG 1 cut(s) 399
BslFI GGGAC 1 cut(s) 638
BslI CCNNNNNNNGG 2 cut(s) 148, 782
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 638
BsnI GGCC 1 cut(s) 753
BsoBI CYCGRG 1 cut(s) 398
Bsp119I TTCGAA 1 cut(s) 178
Bsp1286I GDGCHC 2 cut(s) 312, 498
Bsp143I GATC 2 cut(s) 137, 679
BspACI CCGC 5 cut(s) 190, 300, 533, 715, 797
BspANI GGCC 1 cut(s) 753
BspCNI CTCAG 2 cut(s) 33, 39
BspLI GGNNCC 2 cut(s) 349, 457
BspPI GGATC 1 cut(s) 132
BspQI GCTCTTC 1 cut(s) 48
BspT104I TTCGAA 1 cut(s) 178
BsrI ACTGG 1 cut(s) 356
BssECI CCNNGG 1 cut(s) 398
BssMI GATC 2 cut(s) 137, 679
BssNAI GTATAC 1 cut(s) 565
BssSI CACGAG 1 cut(s) 146
Bst1107I GTATAC 1 cut(s) 565
Bst2BI CACGAG 1 cut(s) 146
Bst4CI ACNGT 4 cut(s) 175, 469, 478, 629
Bst6I CTCTTC 2 cut(s) 48, 115
BstAPI GCANNNNNTGC 1 cut(s) 285
BstBI TTCGAA 1 cut(s) 178
BstC8I GCNNGC 2 cut(s) 14, 312
BstDEI CTNAG 4 cut(s) 20, 26, 80, 734
BstF5I GGATG 1 cut(s) 104
BstKTI GATC 2 cut(s) 140, 682
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 2 cut(s) 137, 679
BstMWI GCNNNNNNNGC 2 cut(s) 285, 530
BstSCI CCNGG 2 cut(s) 397, 398
BstSFI CTRYAG 1 cut(s) 675
BstV1I GCAGC 4 cut(s) 17, 193, 300, 557
BstX2I RGATCY 2 cut(s) 137, 679
BstYI RGATCY 2 cut(s) 137, 679
BstZ17I GTATAC 1 cut(s) 565
BsuRI GGCC 1 cut(s) 753
BtsCI GGATG 1 cut(s) 104
BtsIMutI CAGTG 1 cut(s) 171
Cac8I GCNNGC 2 cut(s) 14, 312
Cfr13I GGNCC 1 cut(s) 455
Cfr9I CCCGGG 1 cut(s) 398
Csp6I GTAC 1 cut(s) 359
CviAII CATG 2 cut(s) 442, 632
CviQI GTAC 1 cut(s) 359
DdeI CTNAG 4 cut(s) 20, 26, 80, 734
DpnI GATC 2 cut(s) 139, 681
DpnII GATC 2 cut(s) 137, 679
DriI GACNNNNNGTC 1 cut(s) 461
Eam1104I CTCTTC 2 cut(s) 48, 115
Eam1105I GACNNNNNGTC 1 cut(s) 461
EarI CTCTTC 2 cut(s) 48, 115
Ecl136II GAGCTC 2 cut(s) 310, 496
Eco24I GRGCYC 2 cut(s) 312, 498
Eco47I GGWCC 1 cut(s) 455
Eco53kI GAGCTC 2 cut(s) 310, 496
Eco88I CYCGRG 1 cut(s) 398
EcoICRI GAGCTC 2 cut(s) 310, 496
EcoO109I RGGNCCY 1 cut(s) 455
EcoT38I GRGCYC 2 cut(s) 312, 498
FaeI CATG 2 cut(s) 445, 635
FaiI YATR 8 cut(s) 254, 330, 405, 435, 443, 565, 614, 633
FaqI GGGAC 1 cut(s) 638
FatI CATG 2 cut(s) 441, 631
FauI CCCGC 1 cut(s) 722
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 5 cut(s) 31, 207, 289, 300, 546
FokI GGATG 1 cut(s) 91
FriOI GRGCYC 2 cut(s) 312, 498
Fsp4HI GCNGC 5 cut(s) 31, 207, 289, 300, 546
FspBI CTAG 1 cut(s) 542
GluI GCNGC 5 cut(s) 31, 207, 289, 300, 546
GsuI CTGGAG 1 cut(s) 759
HaeIII GGCC 1 cut(s) 753
HapII CCGG 1 cut(s) 399
Hin1II CATG 2 cut(s) 445, 635
HindIII AAGCTT 1 cut(s) 82
HinfI GANTC 3 cut(s) 242, 518, 761
HpaII CCGG 1 cut(s) 399
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 3 cut(s) 241, 352, 452
Hpy188III TCNNGA 3 cut(s) 224, 246, 392
Hpy8I GTNNAC 1 cut(s) 565
Hpy99I CGWCG 2 cut(s) 215, 393
HpyAV CCTTC 3 cut(s) 75, 338, 664
HpyCH4III ACNGT 4 cut(s) 175, 469, 478, 629
HpyCH4IV ACGT 2 cut(s) 200, 691
HpyCH4V TGCA 4 cut(s) 288, 524, 590, 608
HpyF10VI GCNNNNNNNGC 2 cut(s) 285, 530
HpyF3I CTNAG 4 cut(s) 20, 26, 80, 734
HpySE526I ACGT 2 cut(s) 200, 691
Hsp92II CATG 2 cut(s) 445, 635
Kzo9I GATC 2 cut(s) 137, 679
LguI GCTCTTC 1 cut(s) 48
LmnI GCTCC 3 cut(s) 38, 501, 553
Lsp1109I GCAGC 4 cut(s) 17, 193, 300, 557
MaeI CTAG 1 cut(s) 542
MaeII ACGT 2 cut(s) 200, 691
MaeIII GTNAC 2 cut(s) 463, 799
MalI GATC 2 cut(s) 139, 681
MboI GATC 2 cut(s) 137, 679
MboII GAAGA 6 cut(s) 35, 102, 105, 108, 494, 770
MflI RGATCY 2 cut(s) 137, 679
MhlI GDGCHC 2 cut(s) 312, 498
MluCI AATT 3 cut(s) 49, 407, 664
MmeI TCCRAC 3 cut(s) 258, 375, 408
MnlI CCTC 8 cut(s) 46, 85, 112, 118, 193, 446, 561, 669
MseI TTAA 5 cut(s) 53, 155, 581, 770, 813
MspA1I CMGCKG 1 cut(s) 30
MspI CCGG 1 cut(s) 399
MspR9I CCNGG 2 cut(s) 399, 400
MwoI GCNNNNNNNGC 2 cut(s) 285, 530
NciI CCSGG 2 cut(s) 399, 400
NdeII GATC 2 cut(s) 137, 679
NlaIII CATG 2 cut(s) 445, 635
NlaIV GGNNCC 2 cut(s) 349, 457
NmuCI GTSAC 2 cut(s) 463, 799
NspV TTCGAA 1 cut(s) 178
PciSI GCTCTTC 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PfeI GAWTC 3 cut(s) 242, 518, 761
PflMI CCANNNNNTGG 1 cut(s) 148
PkrI GCNGC 5 cut(s) 32, 208, 290, 301, 547
PpuMI RGGWCCY 1 cut(s) 455
Psp124BI GAGCTC 2 cut(s) 312, 498
Psp1406I AACGTT 1 cut(s) 691
Psp5II RGGWCCY 1 cut(s) 455
PspN4I GGNNCC 2 cut(s) 349, 457
PspPI GGNCC 1 cut(s) 455
PspPPI RGGWCCY 1 cut(s) 455
PsuI RGATCY 2 cut(s) 137, 679
PvuII CAGCTG 1 cut(s) 30
RsaI GTAC 1 cut(s) 360
RsaNI GTAC 1 cut(s) 359
SacI GAGCTC 2 cut(s) 312, 498
SapI GCTCTTC 1 cut(s) 48
SaqAI TTAA 5 cut(s) 53, 155, 581, 770, 813
SatI GCNGC 5 cut(s) 31, 207, 289, 300, 546
Sau3AI GATC 2 cut(s) 137, 679
Sau96I GGNCC 1 cut(s) 455
ScrFI CCNGG 2 cut(s) 399, 400
SduI GDGCHC 2 cut(s) 312, 498
SfcI CTRYAG 1 cut(s) 675
SfuI TTCGAA 1 cut(s) 178
SinI GGWCC 1 cut(s) 455
SmaI CCCGGG 1 cut(s) 400
Sse9I AATT 3 cut(s) 49, 407, 664
SsiI CCGC 5 cut(s) 190, 300, 533, 715, 797
SspMI CTAG 1 cut(s) 542
SstI GAGCTC 2 cut(s) 312, 498
StyD4I CCNGG 2 cut(s) 397, 398
TaaI ACNGT 4 cut(s) 175, 469, 478, 629
TaiI ACGT 2 cut(s) 203, 694
TaqI TCGA 5 cut(s) 178, 213, 225, 391, 528
TasI AATT 3 cut(s) 49, 407, 664
TauI GCSGC 1 cut(s) 302
TfiI GAWTC 3 cut(s) 242, 518, 761
Tru1I TTAA 5 cut(s) 53, 155, 581, 770, 813
Tru9I TTAA 5 cut(s) 53, 155, 581, 770, 813
TscAI CASTG 1 cut(s) 178
TseFI GTSAC 2 cut(s) 463, 799
TseI GCWGC 4 cut(s) 30, 206, 288, 545
Tsp45I GTSAC 2 cut(s) 463, 799
TspDTI ATGAA 4 cut(s) 231, 395, 411, 420
TspGWI ACGGA 1 cut(s) 377
TspMI CCCGGG 1 cut(s) 398
TspRI CASTG 1 cut(s) 178
Van91I CCANNNNNTGG 1 cut(s) 148
VpaK11BI GGWCC 1 cut(s) 455
XapI RAATTY 1 cut(s) 407
XmaI CCCGGG 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 564
XspI CTAG 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.