Rroxscaffold_1G00000650

NusB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010441
Physical Location & Seq
Forward (+)
1075687 .. 1082816
7130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00000650.1

Sequence Viewer

Length: 861 bp
ATGGAGGGAAGCTCGCTTCTCAGTCTCAGCTGCTCCTCTGCTCTTCCCAGTTTGATACCCACTCCCTCTTACTCCTCTCTAAGCTTCAACTCCCGCTCCTCGTCTTCTTCTTCTTTGCTGTTCGGAAAATCATATCTCGTGGGTTTAATCCCAAGACCATTCACTGTTCGAACCTCTCTCCGCACTTCCACGTTCGCCGCCGTCGATGAAGCTCTCGAAAAGCCCGACTCTGATTCCAGAGATATGTTGCCCAAGATTGACAAGAGTGGCAGATTTTGCAGCCCCAGAGCCGCCAGAGAGCTCGCTCTCTCCATTATTTATGCTTCCTGCTTAGAAGGTTCCGACCCAGTAAGGTTATTTGAAAAGCGAATGAATGTCCGTCGAGAACCCGGGTATGAATTTGACAAGGCTTCATTGTTGGAATATAATCCCATGAGCTTTGGAGGACCCCCTGTCACAGTTCAAACTGTTGAGGAAGCAGATGAGCTCCTGCTCAATGACGAAAAGGAATCTGCAATCGAAGCGGAAGTTCTTGCAGCTCCTCCAAAGTTGGTATACAGCAAACTGATTTTAAGATTTGCAAGGAAACTTTTGGTTGCAGTTATGGATAAATGGGACAGTCATGTGCTTGTCATTGACAGAGTTGCCCCTCCAAATTGGAAGGATGAGCCAGGAAGCAGAATCTTGGAGCTTTGCATCCTTCACTTGGCAATGTCAGAAATAACAGTGTTAGGAACGCGACACCAGATTGTCATTAATGAGGCTGTAGATCTTGCTAAACGTTTCTGTGATGGGTCAGCCCCCGCACAATCAATGGTTGTCTTAGAACATTCGTCAATGGCCTTGAAGAATCTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NusB

Protein Analysis

286

Amino Acids

31.45

Weight (kDa)

5.7

Isoelectric Point (pI)

49.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26370 AT4G26370 AT4G26370
fragaria_vesca FvH4_3g45910 FvH4_3g45910 FvH4_3g45910
malus_domestica MD11G1001800.v1.1
prunus_persica Prupe.6G001800_v2.0.a1
pyrus_communis pycom11g00180
rosa_chinensis RchiOBHm_Chr5g0084001
rosa_laevigata RLG00000037071
rosa_multiflora Rmu_sc0001137.1_g000001 Rmu_ssc0000372.1_g000077
rosa_roxburghii Rroxscaffold_1G00000650
rosa_rugosa Rorug05G0488200 Rorug05G0488300
rosa_samantha Rh5AG540900 Rh5BG566200 Rh5CG588200 Rh5DG574500
rosa_wichuraiana Rw5G050300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 96
AccI GTMKAC 1 cut(s) 555
AccII CGCG 1 cut(s) 739
AciI CCGC 6 cut(s) 94, 181, 198, 291, 524, 804
AclI AACGTT 1 cut(s) 781
AcsI RAATTY 1 cut(s) 398
AfiI CCNNNNNNNGG 1 cut(s) 706
AgsI TTSAA 4 cut(s) 88, 362, 464, 847
AhdI GACNNNNNGTC 1 cut(s) 452
AjnI CCWGG 1 cut(s) 670
AluBI AGCT 9 cut(s) 12, 30, 84, 212, 301, 438, 487, 539, 691
AluI AGCT 9 cut(s) 12, 30, 84, 212, 301, 438, 487, 539, 691
Alw21I GWGCWC 2 cut(s) 303, 489
Alw26I GTCTC 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 389
AoxI GGCC 1 cut(s) 840
ApeKI GCWGC 3 cut(s) 30, 279, 536
ApoI RAATTY 1 cut(s) 398
AseI ATTAAT 1 cut(s) 756
AspS9I GGNCC 1 cut(s) 446
AsuC2I CCSGG 2 cut(s) 390, 391
AsuII TTCGAA 1 cut(s) 169
AvaI CYCGRG 1 cut(s) 389
AvaII GGWCC 1 cut(s) 446
BanII GRGCYC 2 cut(s) 303, 489
BauI CACGAG 1 cut(s) 137
BbsI GAAGAC 1 cut(s) 96
Bbv12I GWGCWC 2 cut(s) 303, 489
BbvI GCAGC 3 cut(s) 17, 291, 548
BccI CCATC 1 cut(s) 785
BceAI ACGGC 1 cut(s) 185
BciT130I CCWGG 1 cut(s) 672
BcnI CCSGG 2 cut(s) 390, 391
BcoDI GTCTC 1 cut(s) 29
BfmI CTRYAG 1 cut(s) 765
BglII AGATCT 1 cut(s) 769
BisI GCNGC 5 cut(s) 31, 198, 280, 291, 537
BlsI GCNGC 5 cut(s) 32, 199, 281, 292, 538
Bme1390I CCNGG 3 cut(s) 390, 391, 672
Bme18I GGWCC 1 cut(s) 446
BmeRI GACNNNNNGTC 1 cut(s) 452
BmeT110I CYCGRG 1 cut(s) 389
BmgT120I GGNCC 1 cut(s) 446
BmiI GGNNCC 2 cut(s) 340, 448
BmrFI CCNGG 3 cut(s) 390, 391, 672
BmrI ACTGGG 2 cut(s) 42, 341
BmsI GCATC 1 cut(s) 705
BmuI ACTGGG 2 cut(s) 42, 341
BpiI GAAGAC 1 cut(s) 96
BplI GAGNNNNNCTC 1 cut(s) 28
Bpu14I TTCGAA 1 cut(s) 169
BpuMI CCSGG 2 cut(s) 390, 391
BsaJI CCNNGG 1 cut(s) 389
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 1 cut(s) 706
Bse1I ACTGG 2 cut(s) 48, 347
Bse3DI GCAATG 1 cut(s) 717
BseBI CCWGG 1 cut(s) 672
BseDI CCNNGG 1 cut(s) 389
BseGI GGATG 2 cut(s) 670, 696
BseLI CCNNNNNNNGG 1 cut(s) 706
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 2 cut(s) 34, 40
BseNI ACTGG 2 cut(s) 48, 347
BseRI GAGGAG 4 cut(s) 25, 64, 88, 531
BseXI GCAGC 3 cut(s) 17, 291, 548
Bsh1236I CGCG 1 cut(s) 739
BshFI GGCC 1 cut(s) 842
BsiHKAI GWGCWC 2 cut(s) 303, 489
BsiHKCI CYCGRG 1 cut(s) 389
BsiSI CCGG 1 cut(s) 390
BslFI GGGAC 1 cut(s) 629
BslI CCNNNNNNNGG 1 cut(s) 706
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 629
BsnI GGCC 1 cut(s) 842
BsoBI CYCGRG 1 cut(s) 389
Bsp119I TTCGAA 1 cut(s) 169
Bsp1286I GDGCHC 2 cut(s) 303, 489
Bsp143I GATC 1 cut(s) 769
BspACI CCGC 6 cut(s) 94, 181, 198, 291, 524, 804
BspANI GGCC 1 cut(s) 842
BspCNI CTCAG 2 cut(s) 33, 39
BspFNI CGCG 1 cut(s) 739
BspLI GGNNCC 2 cut(s) 340, 448
BspQI GCTCTTC 1 cut(s) 48
BspT104I TTCGAA 1 cut(s) 169
BsrBI CCGCTC 1 cut(s) 96
BsrDI GCAATG 1 cut(s) 717
BsrI ACTGG 2 cut(s) 48, 347
BssECI CCNNGG 1 cut(s) 389
BssMI GATC 1 cut(s) 769
BssNAI GTATAC 1 cut(s) 556
BssSI CACGAG 1 cut(s) 137
Bst1107I GTATAC 1 cut(s) 556
Bst2BI CACGAG 1 cut(s) 137
Bst2UI CCWGG 1 cut(s) 672
Bst4CI ACNGT 5 cut(s) 166, 460, 469, 620, 727
Bst6I CTCTTC 1 cut(s) 48
BstAPI GCANNNNNTGC 1 cut(s) 276
BstBI TTCGAA 1 cut(s) 169
BstC8I GCNNGC 2 cut(s) 14, 303
BstDEI CTNAG 5 cut(s) 20, 26, 80, 331, 823
BstF5I GGATG 2 cut(s) 670, 696
BstFNI CGCG 1 cut(s) 739
BstKTI GATC 1 cut(s) 772
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 1 cut(s) 769
BstMWI GCNNNNNNNGC 2 cut(s) 276, 521
BstNI CCWGG 1 cut(s) 672
BstSCI CCNGG 3 cut(s) 388, 389, 670
BstSFI CTRYAG 1 cut(s) 765
BstUI CGCG 1 cut(s) 739
BstV1I GCAGC 3 cut(s) 17, 291, 548
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 1 cut(s) 769
BstYI RGATCY 1 cut(s) 769
BstZ17I GTATAC 1 cut(s) 556
BsuRI GGCC 1 cut(s) 842
BtsCI GGATG 2 cut(s) 670, 696
BtsIMutI CAGTG 2 cut(s) 162, 732
Cac8I GCNNGC 2 cut(s) 14, 303
Cfr13I GGNCC 1 cut(s) 446
Cfr9I CCCGGG 1 cut(s) 389
CviAII CATG 2 cut(s) 433, 623
DdeI CTNAG 5 cut(s) 20, 26, 80, 331, 823
DpnI GATC 1 cut(s) 771
DpnII GATC 1 cut(s) 769
DriI GACNNNNNGTC 1 cut(s) 452
Eam1104I CTCTTC 1 cut(s) 48
Eam1105I GACNNNNNGTC 1 cut(s) 452
EarI CTCTTC 1 cut(s) 48
Ecl136II GAGCTC 2 cut(s) 301, 487
Eco24I GRGCYC 2 cut(s) 303, 489
Eco47I GGWCC 1 cut(s) 446
Eco53kI GAGCTC 2 cut(s) 301, 487
Eco88I CYCGRG 1 cut(s) 389
EcoICRI GAGCTC 2 cut(s) 301, 487
EcoO109I RGGNCCY 1 cut(s) 446
EcoRII CCWGG 1 cut(s) 670
EcoT38I GRGCYC 2 cut(s) 303, 489
FaeI CATG 2 cut(s) 436, 626
FaiI YATR 9 cut(s) 133, 245, 321, 396, 426, 434, 556, 605, 624
FaqI GGGAC 1 cut(s) 629
FatI CATG 2 cut(s) 432, 622
FauI CCCGC 2 cut(s) 101, 811
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 5 cut(s) 31, 198, 280, 291, 537
FokI GGATG 2 cut(s) 677, 683
FriOI GRGCYC 2 cut(s) 303, 489
Fsp4HI GCNGC 5 cut(s) 31, 198, 280, 291, 537
GluI GCNGC 5 cut(s) 31, 198, 280, 291, 537
HaeIII GGCC 1 cut(s) 842
HapII CCGG 1 cut(s) 390
Hin1II CATG 2 cut(s) 436, 626
HindIII AAGCTT 1 cut(s) 82
HinfI GANTC 5 cut(s) 227, 233, 509, 681, 850
HpaII CCGG 1 cut(s) 390
Hpy166II GTNNAC 1 cut(s) 556
Hpy188I TCNGA 4 cut(s) 125, 232, 343, 718
Hpy188III TCNNGA 3 cut(s) 215, 237, 383
Hpy8I GTNNAC 1 cut(s) 556
Hpy99I CGWCG 2 cut(s) 206, 384
HpyAV CCTTC 3 cut(s) 329, 655, 710
HpyCH4III ACNGT 5 cut(s) 166, 460, 469, 620, 727
HpyCH4IV ACGT 2 cut(s) 191, 781
HpyCH4V TGCA 6 cut(s) 279, 515, 536, 581, 599, 696
HpyF10VI GCNNNNNNNGC 2 cut(s) 276, 521
HpyF3I CTNAG 5 cut(s) 20, 26, 80, 331, 823
HpySE526I ACGT 2 cut(s) 191, 781
Hsp92II CATG 2 cut(s) 436, 626
Kzo9I GATC 1 cut(s) 769
LguI GCTCTTC 1 cut(s) 48
LmnI GCTCC 5 cut(s) 38, 101, 492, 544, 688
Lsp1109I GCAGC 3 cut(s) 17, 291, 548
LweI GCATC 1 cut(s) 705
MaeII ACGT 2 cut(s) 191, 781
MaeIII GTNAC 1 cut(s) 454
MalI GATC 1 cut(s) 771
MbiI CCGCTC 1 cut(s) 96
MboI GATC 1 cut(s) 769
MboII GAAGA 5 cut(s) 35, 96, 99, 102, 859
MflI RGATCY 1 cut(s) 769
MhlI GDGCHC 2 cut(s) 303, 489
MluCI AATT 2 cut(s) 398, 655
MlyI GAGTC 1 cut(s) 221
MmeI TCCRAC 2 cut(s) 366, 399
MseI TTAA 4 cut(s) 146, 572, 756, 859
MspA1I CMGCKG 1 cut(s) 30
MspI CCGG 1 cut(s) 390
MspR9I CCNGG 3 cut(s) 390, 391, 672
MvaI CCWGG 1 cut(s) 672
MvnI CGCG 1 cut(s) 739
MwoI GCNNNNNNNGC 2 cut(s) 276, 521
NciI CCSGG 2 cut(s) 390, 391
NdeII GATC 1 cut(s) 769
NlaIII CATG 2 cut(s) 436, 626
NlaIV GGNNCC 2 cut(s) 340, 448
NmuCI GTSAC 1 cut(s) 454
NspV TTCGAA 1 cut(s) 169
PciSI GCTCTTC 1 cut(s) 48
PcsI WCGNNNNNNNCGW 2 cut(s) 201, 222
PfeI GAWTC 4 cut(s) 233, 509, 681, 850
PkrI GCNGC 5 cut(s) 32, 199, 281, 292, 538
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
PpuMI RGGWCCY 1 cut(s) 446
PshBI ATTAAT 1 cut(s) 756
Psp124BI GAGCTC 2 cut(s) 303, 489
Psp1406I AACGTT 1 cut(s) 781
Psp5II RGGWCCY 1 cut(s) 446
Psp6I CCWGG 1 cut(s) 670
PspGI CCWGG 1 cut(s) 670
PspN4I GGNNCC 2 cut(s) 340, 448
PspPI GGNCC 1 cut(s) 446
PspPPI RGGWCCY 1 cut(s) 446
PsuI RGATCY 1 cut(s) 769
PvuII CAGCTG 1 cut(s) 30
SacI GAGCTC 2 cut(s) 303, 489
SapI GCTCTTC 1 cut(s) 48
SaqAI TTAA 4 cut(s) 146, 572, 756, 859
SatI GCNGC 5 cut(s) 31, 198, 280, 291, 537
Sau3AI GATC 1 cut(s) 769
Sau96I GGNCC 1 cut(s) 446
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 3 cut(s) 390, 391, 672
SduI GDGCHC 2 cut(s) 303, 489
SfaNI GCATC 1 cut(s) 705
SfcI CTRYAG 1 cut(s) 765
SfuI TTCGAA 1 cut(s) 169
SinI GGWCC 1 cut(s) 446
SmaI CCCGGG 1 cut(s) 391
Sse9I AATT 2 cut(s) 398, 655
SsiI CCGC 6 cut(s) 94, 181, 198, 291, 524, 804
SstI GAGCTC 2 cut(s) 303, 489
StyD4I CCNGG 3 cut(s) 388, 389, 670
TaaI ACNGT 5 cut(s) 166, 460, 469, 620, 727
TaiI ACGT 2 cut(s) 194, 784
TaqI TCGA 5 cut(s) 169, 204, 216, 382, 519
TasI AATT 2 cut(s) 398, 655
TauI GCSGC 2 cut(s) 200, 293
TfiI GAWTC 4 cut(s) 233, 509, 681, 850
Tru1I TTAA 4 cut(s) 146, 572, 756, 859
Tru9I TTAA 4 cut(s) 146, 572, 756, 859
TscAI CASTG 2 cut(s) 169, 732
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 3 cut(s) 30, 279, 536
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 4 cut(s) 222, 386, 402, 411
TspGWI ACGGA 1 cut(s) 368
TspMI CCCGGG 1 cut(s) 389
TspRI CASTG 2 cut(s) 169, 732
VpaK11BI GGWCC 1 cut(s) 446
VspI ATTAAT 1 cut(s) 756
XapI RAATTY 1 cut(s) 398
XmaI CCCGGG 1 cut(s) 389
XmiI GTMKAC 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.